CN103969258B - Test strips and methods - Google Patents

Test strips and methods Download PDF

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Publication number
CN103969258B
CN103969258B CN 201410217634 CN201410217634A CN103969258B CN 103969258 B CN103969258 B CN 103969258B CN 201410217634 CN201410217634 CN 201410217634 CN 201410217634 A CN201410217634 A CN 201410217634A CN 103969258 B CN103969258 B CN 103969258B
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cadmium
solution
color
ix
nbt
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CN 201410217634
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CN103969258A (en )
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温广明
刘斌
双少敏
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山西大学
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Abstract

本发明提供了一种裸眼快速检测Cd2+的试纸和方法,是利用量子点在可见光照射下产生光电子使染料变色的原理发明一种新的检测试纸和方法,所制得的试纸成本极低,其能定性或半定量检测食品或水中的镉,测定速度快,灵敏度高。 The present invention provides a test strip for Cd2 + and rapid detection method for the naked eye, is the use of quantum dots generated photoelectrons under visible irradiation discoloration of the dye of the present invention a new test strip and method, the prepared paper is extremely low cost, which cadmium can be qualitative or semi-quantitative detection of food or water, measuring speed, and high sensitivity. 检测方法简单,对操作人员技术要求低,非常适合现场检测。 Detection method is simple, low technical requirements for the operator, suitable for field testing.

Description

一种裸眼快速检测Cd2+的试纸和方法 The naked eye rapid detection test strips and methods of Cd2 +

一、技术领域 First, the technical field

[0001]本发明涉及检测重金属离子的试纸和方法,具体是一种裸眼快速检测Cd2+的试纸和方法。 [0001] The present invention relates to a method and test strip for detecting heavy metal ions, in particular an auto test strip for rapid detection of Cd2 + and methods.

二、背景技术 Second, the technical background

[0002]镉是我国重点控制的重金属污染物,属于第一类污染物。 [0002] Cadmium is a focus control of heavy metal pollutants, contaminants belonging to the first category. 除了可造成急、慢性中毒,还有一定的致畸、致癌和致突变作用,其最主要的危害还是在体内蓄积造成的慢性中毒。 In addition to causing acute and chronic poisoning, there is a certain teratogenic, carcinogenic and mutagenic effect, its main hazard or chronic poisoning caused by the accumulation in the body. 机体摄入镉的最主要途径是食品,而食品中的镉主要来自环境污染。 The main way the body's intake of cadmium is food, and cadmium in food, mainly from environmental pollution. 因此,监测环境中镉的含量,是控制人体镉摄入量,预防和减少镉对人体危害的重要措施。 Therefore, the content of cadmium in environment monitoring, control human intake of cadmium, cadmium important measures to prevent and reduce harm to human body.

[0003]镉的检测方法很多,如:分光光度法、原子吸收法、极谱催化波法、单扫描极谱法、溶出伏安法、电位溶出法、电感耦合等离子体发射光谱法、中子活化分析法、质谱法、色谱法、电化学生物传感器法以及各种方法的联用技术等,它们大多需要昂贵的仪器,并且操作复杂,需要专门的技术人员来完成。 [0003] The method of detecting cadmium many, such as: spectrophotometry, atomic absorption spectrometry, catalytic wave method, a single sweep polarography, stripping voltammetry, potential stripping analysis, inductively coupled plasma emission spectrometry, neutron activation analysis, mass spectrometry, chromatography, and electrochemical biosensors method Coupled with various methods like, which often require expensive equipment, complex operation and requires special skill to complete.

三、发明内容 III. SUMMARY OF THE INVENTION

[0004]本发明的目的在于提供一种裸眼快速检测Cd2+的试纸和方法,这种试纸和方应能定性或半定量检测食品或水中的镉,不仅可节约大量的人力物力和财力,且试纸携带方便,方法简单,非常适合现场检测。 [0004] The object of the present invention is to Cd2 + in test strips and a method for providing an auto rapid detection, test strips, and shall be able to qualitative or semi-quantitative cadmium detection of food or water, not only can save a lot of manpower and financial resources, and paper easy to carry, simple, very suitable for field testing.

[0005]本发明提供的一种裸眼快速检测Cd2+的试纸,通过如下方法制得: [0005] The present invention provides an auto rapid detection of Cd2 + paper, prepared by the following method:

[0006] (I)以0.1M他$的乙醇水溶液和0.1M的Zn (Ac)2的乙醇水溶液按体积比1:1合成ZnS量子点水溶液,所述的乙醇与水的体积比为1: 3;配置浓度为lmg/mL的硝基四氮唑蓝(NBT)水溶液; [0006] (I) in his $ 0.1M aqueous solution of ethanol and 0.1M of Zn (Ac) 2 aqueous ethanol by volume ratio of 1: 1 Synthesis of ZnS quantum dot solution, the volume ratio of ethanol to water is 1: 3; configure concentration of lmg / mL nitro blue tetrazolium (NBT) solution;

[0007] (2)将ZnS量子点水溶液涂布于玻璃纤维纸上,将NBT水溶液划刻在条状硝酸纤维膜上,置干; [0007] (2) an aqueous solution of the ZnS QDs coated glass fiber paper, the fibers NBT solution scribing nitrocellulose membrane strip, home dry;

[0008] (3)将划刻好的硝酸纤维膜、已涂布ZnS量子点的玻璃纤维纸依次粘贴于硬牛皮卡纸上,在玻璃纤维纸下方即卡纸末端粘贴聚酯纤维作为样品垫,之后将粘贴好的卡纸沿垂直于NBT划痕线的纵向方向裁剪成条状试纸,NBT划痕所在位置为指示区。 [0008] (3) The good scribe nitrocellulose membranes, glass fiber paper has been coated ZnS QDs are sequentially attached to hard paper pickup cow, i.e. the end of the jam paste polyester fibers under the sample pad as glass fiber paper , after pasting the good jam direction perpendicular to the longitudinal direction of the scribe line NBT test paper was cut into strips, NBT region indicating the location of the scratch.

[0009] 所述的玻璃纤维也可以是聚酯纤维、醋酸纤维、硝酸纤维。 [0009] The glass fibers may be polyester fibers, cellulose acetate, nitrocellulose.

[0010]本发明提供的一种裸眼快速检测Cd2+的方法,步骤包括: [0010] The present invention provides an auto Cd2 + in the rapid detection method, comprising the step of:

[0011] (I)将上述制成的试纸条插入待测液中,虹吸30秒到10分钟,在阳光或其它可见光照射下读取指示区的颜色; [0011] (I) made of the above test strip inserted into the test solution, the siphon 30 seconds to 10 minutes and read the color area indicated in the sun or other visible light irradiation;

[0012] (2)根据指示区的颜色判断待测样镉离子的含量:若指示区颜色为深蓝色,表明待测溶液中不含镉离子或含量极微;若指示区颜色为浅蓝,表明镉离子含量在IX 10—6M〜I X10—4M之间;若颜色为黄色或无色,表明镉离子含量在IX 10—4M至IX 10—2M之间。 [0012] (2) determining the color content of the sample under test indication region cadmium ion: If the color is dark blue indicator region indicating that the test solution does not contain cadmium or minimal content; light blue color if the indicator zones, showed that the content of cadmium between IX 10-6M~I X10-4M; if the color is yellow or colorless, indicating that the content of cadmium between IX 10-4M to IX 10-2M.

[0013]本发明利用了镉离子对量子点具有特异性破坏作用从而抑制了其光电变色过程而进行测定。 [0013] The present invention utilizes a cadmium specific damaging effect on the quantum dots is suppressed and the photoelectric color measurement process. 所制得的试纸成本极低,测定速度快,灵敏度高。 The prepared test strips low cost, fast measurement speed and high sensitivity. 同时操作十分简便,对操作人员技术要求低。 While the operation is very simple, low technical requirements of the operator. 适合于环境监测、现场测定等场合中镉离子的检测。 Suitable for the detection of cadmium ions in the case of environmental monitoring, on-site measurement and the like.

四、附图说明 IV BRIEF DESCRIPTION

[0014]图1本发明一种裸眼快速检测Cd2+的试纸结构示意图 [0014] FIG 1 an inventive fast naked eye and Cd2 + schematic view of the detection strip

五、具体实施方式 V. DETAILED DESCRIPTION

[0015] 实施例1. [0015] Example 1.

[0016] (I)以Na2S为硫源合成ZnS量子点。 [0016] (I) a sulfur source in the synthesis of Na2S ZnS quantum dots. 具体操作方法为,分別配制0.1M的Zn(Ac)2和 Specific methods of operation, were prepared in 0.1M Zn (Ac) 2, and

0.1M的Na2S乙醇水溶液各50mL〔其中水与乙醇体积比為3:1〕,用HAc调节Zn(Ac)2.水与乙醇溶液的PH=5 ο室溫搅拌下将Zn (Ac) 2水与乙醇溶液逐滴加入Na2S水与醇溶液中(控制滴速为每分种2mL〜3mL),滴毕继续搅拌反应30分种。 0.1M Na2S solution in ethanol [wherein each of 50mL of water and ethanol volume ratio of 3: 1], adjusting Zn (Ac) with aqueous ethanol HAc 2 PH = 5 ο solution at room temperature under stirring Zn (Ac) 2 water. ethanol was added dropwise a solution of water and alcohol Na2S (as controlled drip rate per minute 2mL~3mL), dropwise. the reaction was stirred 30 minutes. 离心分离,沉淀分別用水和无水乙醇洗涤3次,40°C真空干燥2小时可得ZnS量子点,在4°C条件下储存备用,可储存3个月。 Centrifugation, the precipitate was washed with water and ethanol 3 times, 40 ° C and dried in vacuo 2 hours to obtain ZnS quantum dot, stored at 4 ° C for the standby condition, can be stored for three months.

[0017] (2)将NBT粉末配成水溶液。 [0017] (2) an aqueous solution of the formulated powder NBT.

[0018] (3)将ZnS量子点水溶液涂布于玻璃纤维垫上,将NBT水溶液划刻在硝酸纤维膜上, [0018] (3) an aqueous solution of the ZnS QDs coated glass fiber mat, the NBT solution scribing nitrocellulose membrane,

置干; Home dry;

[0019] (4)将划刻好的硝酸纤维膜、已涂布ZnS量子点的玻璃纤维纸依次粘贴于硬牛皮卡纸上,在玻璃纤维纸下方即卡纸末端粘贴聚酯纤维作为样品垫。 [0019] (4) The good scribe nitrocellulose membranes, glass fiber paper has been coated ZnS QDs are sequentially attached to hard paper pickup cow, i.e. the end of the jam paste polyester fibers under the sample pad as glass fiber paper . 之后将粘贴好的卡纸沿垂直于NBT划痕线的纵向方向裁剪成条状试纸。 After pasting the good cut into strips perpendicular to jam along the longitudinal direction of the strip to the scribe line NBT. NBT划痕所在位置为指示区(即图1中的指示线)。 NBT scribe region indicating the location (i.e., the line indicated in FIG. 1).

[0020]将所制得的试纸的样品垫区浸入浓度为I X 10—4M的醋酸镉溶液中,虹吸3分钟后放置于日光下照射。 [0020] The test strips prepared sample pad region immersed in the solution of cadmium acetate solution I X 10-4M, the siphon is placed under sunlight for 3 minutes. 光照下指示区颜色为无色,判断镉离子浓度在IX 10—4M至IX 10—2M之间。 Indication region under illumination color is colorless, is determined between the cadmium ion concentration IX 10-4M to IX 10-2M.

[0021]实施例2.按实施例1制成试纸,将试纸样品区插入浓度为I X 10—5M的配制镉离子溶液中,虹吸3分钟。 [0021] Example 2. Example 1 is made of paper, the test strip is inserted into the sample region I X concentration of cadmium ions in the solution formulation of 10-5M, siphon 3 minutes. 在灯光下照射,指示线变为浅蓝色,判断镉离子含量在IX 10—6M〜IX 10一4M之间。 In the light irradiation, indicating line changes to light blue, cadmium content is determined IX 10-6M~IX 10 between a 4M.

[0022] 实施例3 [0022] Example 3

[0023] (1)配制含有2112+、1%2+、0&2+^13+、5042—、^)3—、? [0023] (1) formulation contained 2112 + 1% + 2, 2 + ^ & 0 13 +, 5042--, ^) 3- ,? 043—离子浓度各为0.0謂的溶液(不含锦离子)一份。 043- ion concentration in each solution, 0.0 that (Jin free ions) a.

[0024] (2)按实施例1制成试纸,将试纸样品区插入配制好的溶液中,虹吸3分钟。 [0024] (2) in Example 1 is made of paper, the strip is inserted into the prepared solution sample area, the siphon 3 minutes. 在灯光下照射,指示线变为深蓝色,判断溶液中不含镉离子。 In the light irradiation, turned dark blue indicating line, free of cadmium ions in solution is determined.

[0025]实施例4.按实施例1制成试纸,将试纸样品区插入自来水样品中,虹吸3分钟。 [0025] Example 4. Example 1 is made of paper, the test strip is inserted into the sample region in a sample of water, siphon 3 minutes. 在日光下照射,指示线变为深蓝色,判断此自来水样品中不含镉离子。 Under sunlight, turned dark blue indicating line, is determined in this water does not contain cadmium ions in the sample.

[0026]实施例5.取某工厂附近河水样品,初步判断此河水已被排放的镉污染。 [0026] Example 5. The water samples taken near a factory, this preliminary determination of cadmium pollution of river water that has been discharged. 按实施例1中步骤1-4制成试纸,将试纸样品区插入被镉污染的河水样品中,虹吸3分钟。 In one embodiment according to the procedure of Example 1-4 is made of paper, the strip is inserted into the sample zone cadmium in water samples, siphon 3 minutes. 在日光下照射,指示区变为浅蓝色,判断此河水样品中镉离子含量在IX 10—6M〜IX 10—4M之间,已被污染。 Under sunlight, changes to light blue indicator zone, the water in this sample is determined cadmium content is between IX 10-6M~IX 10-4M, it has been contaminated.

[0027]实施例6.按实施例1制成试纸,将试纸样品区插入不知是否被污染的河水样品中,虹吸5分钟。 [0027] Example 6. Example 1 is made of paper, the test strip is inserted into the sample region is unknown whether the sample is contaminated river water, a siphon for 5 minutes. 在日光下照射,指示线变为深蓝色,判断此河水样品中不含镉离子,未被污染。 Under sunlight, turned dark blue indicating line, is determined in this water sample does not contain cadmium, uncontaminated.

[0028]实验证明本发明镉离子检测试纸及方法测定速度快,选择性强,灵敏可靠。 [0028] The present invention proved cadmium test paper and measuring method is faster speed, selectivity, sensitive and reliable. 与传统方法相比,降低了检测成本,提高了检测效率,非常适用于实际样品的快速测定。 Compared with traditional methods, to reduce testing costs, improve the detection efficiency, very suitable for fast real samples.

Claims (3)

  1. 1.一种裸眼快速检测Cd2+的试纸,其特征在于,通过如下步骤的方法制得: (1)以0.1M他^的乙醇水溶液和0.1M的Zn(Ac)2的乙醇水溶液按体积比1:1合成ZnS量子点溶液,所述的乙醇与水的体积比为1:3 ;配置浓度为lmg/mL的NBT水溶液; (2)将ZnS量子点溶液涂布于玻璃纤维纸上,将NBT水溶液划刻在条状硝酸纤维膜上,置干; (3)将划刻好的硝酸纤维膜、已涂布ZnS量子点的玻璃纤维纸依次粘贴于硬牛皮卡纸上,在玻璃纤维纸下方即卡纸末端粘贴聚酯纤维作为样品垫,之后将粘贴好的卡纸沿垂直于NBT划痕线的纵向方向裁剪成条状试纸,NBT划痕所在位置为指示线。 A naked eye test strip for rapid detection of Cd2 +, characterized by the steps of the method: (1) aqueous ethanol at a volume ratio of a 0.1M aqueous solution of ethanol and 0.1M of his Zn ^ of (Ac) 2 is : synthesis of ZnS quantum dot solution 1, the volume ratio of ethanol to water is 1: 3; configure NBT solution concentration lmg / mL; and (2) the coating solution ZnS QDs glass fiber paper, the NBT scribing the fiber aqueous nitrocellulose membrane strips, the dry set; (3) good scribe nitrocellulose membranes, glass fiber paper has been coated ZnS QDs are sequentially attached to hard paper pickup cattle, glass fiber paper below i.e. the end of the jam paste polyester fibers as sample pad, after pasting the good cut into strips perpendicular to jam along the longitudinal direction of the strip to the scribe lines of NBT, NBT scribe line to indicate location.
  2. 2.如权利要求1所述的一种裸眼快速检测Cd2+的试纸,其特征在于,所述的玻璃纤维用聚酯纤维、醋酸纤维或硝酸纤维替代。 2. An naked eye according to claim 1, Cd2 + rapid detection test strip, wherein said glass fibers with polyester fibers, cellulose acetate or nitrocellulose alternative.
  3. 3.一种裸眼快速检测Cd2+的方法,其特征在于,步骤包括: (1)将上述权利要求1或2所述的试纸条插入待测液中,虹吸30秒到10分钟,在阳光或其它可见光照射下读取指示区的颜色; (2)根据指示区的颜色判断待测样镉离子的含量:若指示区颜色为深蓝色,表明待测溶液中不含镉离子或含量极微;若指示区颜色为浅蓝,表明镉离子含量在IX 10—6M〜IX KT4M之间;若颜色为黄色或无色,表明镉离子含量在IX 10—4M至IX 10—2M之间。 3. A method of Cd2 + in rapid detection naked eye, characterized in that, the step comprising: (1) The test strip of claim 12 or claim inserted into the test solution, the siphon 30 seconds to 10 minutes, or sunlight reading the color of the indication region other visible light irradiation; (2) the content of the test samples is determined according to the color cadmium indication region: If the color is dark blue indicator region indicating that the test solution does not contain cadmium or minimal content; If the color is light blue indicator zones indicates the cadmium ion content between IX 10-6M~IX KT4M; if the color is yellow or colorless, indicating that the content of cadmium between IX 10-4M to IX 10-2M.
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CN101962537A (en) * 2010-09-16 2011-02-02 武汉工程大学 Method for preparing (Zn,Cd)S quantum dot
CN102936501A (en) * 2012-11-01 2013-02-20 华东理工大学 Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof
CN103675266A (en) * 2013-11-14 2014-03-26 成都领御生物技术有限公司 Test strip card based on quantum dot labeling

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US7534488B2 (en) * 2003-09-10 2009-05-19 The Regents Of The University Of California Graded core/shell semiconductor nanorods and nanorod barcodes

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Publication number Priority date Publication date Assignee Title
CN101368907A (en) * 2008-07-14 2009-02-18 马义才 Quantum dot marking test strip quantitative detection system based on CMOS image sensor
CN101962537A (en) * 2010-09-16 2011-02-02 武汉工程大学 Method for preparing (Zn,Cd)S quantum dot
CN102936501A (en) * 2012-11-01 2013-02-20 华东理工大学 Quantum dot-organic dye compound closed/open type cadmium ion ratio fluorescent probe and preparation method thereof
CN103675266A (en) * 2013-11-14 2014-03-26 成都领御生物技术有限公司 Test strip card based on quantum dot labeling

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