CN103908692A - Composite biological material patch and preparation method thereof - Google Patents
Composite biological material patch and preparation method thereof Download PDFInfo
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- CN103908692A CN103908692A CN201410124365.5A CN201410124365A CN103908692A CN 103908692 A CN103908692 A CN 103908692A CN 201410124365 A CN201410124365 A CN 201410124365A CN 103908692 A CN103908692 A CN 103908692A
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Abstract
The invention provides a composite biological material patch. The composite biological material patch consists of a base body layer, a wound protecting layer and two release layers, wherein the wound protecting layer is tightly adhered to the center position at one side of the base body layer; one end part of each of the two release layers is covered on the wound protecting layer after being superposed; the wound protecting layer is made from a composite biological material which comprises a silk fibroin porous biological material, chitosan and micro-level inorganic powder particle. By virtue of a combination method of opening, carding, lapping and spunlace and a spraying method, the wound protecting layer is prepared by compounding three substances. The composite biological material patch disclosed by the invention belongs to natural biological material wound patch products, has good biocompatibility, is suitable for various wounds such as burns, scalds and operative incisions, can be used for reducing scars while promoting tissue healing and wound repairing, and has the effect of restraining bacteria on the wound surface from growing; moreover, the composite biological material patch has good flexibility, expansibility and gas permeability, and has stronger bacteriostatic action.
Description
Technical field
The invention belongs to field of medical materials, relate to a kind of wound repair, promote healing and antibacterial composite biological material to apply ointment or plaster, its main component is that fibroin albumen, chitosan, micron order inorganic powder material mix the composite biological material of making by a certain percentage.Be applicable to various wounds and postoperative Wound antibiotic antiinflammatory, hemostasis, promotion organization healing and reparation, reduce cicatrix.
Background technology
The clinical treatment for wound, except operative treatment, also needs to use nanometer that various promotion wounds repair to micron order inorganic powder material, and clinical conventional have raw, the special skin of skin raw etc., then use various medical sanitaries to protect wound product on wound surface.While treatment for the healing of acute and chronic skin wound surface, this product is aseptic microparticle powder body before packaging is opened, and is directly used in wound.When use, please follow following principle: wound surface is carried out to conventional debridement and sterilization disposal.This product is directly sprayed and cover whole wound surface.With aseptic oil gauze or the covering of applying ointment or plaster.Before changing dressings, remove wound surface residue, and repeat above step, until wound healing.As wound surface has infection, need combine use at wound surface place with antibacterials.
In existing medical material, protecting wound product mainly contains: medical cotton ball, cotton swab, binder, adhesive bandage, first-aid kit, absorbent cotton, absorbent carbasus, gauze pad etc., generally have non-sterile and aseptic two kinds.
Wherein, the hospital gauze of sterile manner packaging can directly use, and the gauze of packing in non-sterile mode must can use after the Disinfection Methods such as high temperature and high pressure steam or oxirane.Medical bandage is generally all sold with non-sterilizing medical product.If use medical bandage in the time of wound site, should use with wound isolation.In existing product, aspect aseptic guarantee, majority is to isolate extraneous mode, once there is bacterium to invade, unfavorable wound reparation; Have plenty of artificial interpolation chemistry antibacterial medicines, exist outside certain side effect, cost of manufacture is also higher.
Show according to the study, silkworm silk is made up of the fibroin albumen of internal layer (silk fibroin, SF) and outer coated sericin (secricin), and the former accounts for 70%～80% by content.Research discovery, fibroin albumen is that heavy chain (H chain), light chain (L chain) and p25 molecule were combined into 6: 6: 1.Fibroin albumen has biological degradability, nontoxic, the good premium properties such as human body affinity and biocompatibility, and can be prepared into variform, as cellular, membranaceous and tubulose etc., and along with going deep into Study on Physico-chemical such as its unique amino acid composition and crystalline textures, especially silkworm silk multipurpose exploitation is in recent years to the infiltration of new and high technology, in the exploration of biomedical sector, as utilize fibroin albumen to manufacture engineering material of bone tissue, artificial skin, tissue engineering bracket, slow releasing carrier of medication, biosensor etc., be subject to especially extensive concern, show the application potential of fibroin albumen as bio-medical material.Multinomial achievement in research demonstration, fibroin albumen has stronger natural bacteriostatic effect.
Chitosan in weak acid solvent, be easy to dissolve, it is worthy of note especially dissolve after solution in contain amino (NH2+), these amino are by carrying out anti-bacteria in conjunction with negatron, strong with human body cell affinity, can be absorbed by the body, without any side effects.
Chitosan has the effect that promotes blood coagulation, can be used as hemorrhage.It also can be used for Wound filling material, has sterilizing, promotes wound healing, absorbs Wound exudate, is difficult for the effects such as syneresis.
Using clinically a kind of micron order inorganic powder, as bioactivity glass material, is initiatively repair materials of a kind of typical synthetic wound, is mainly used in treating the skin soft tissue ulcer that a variety of causes causes and promotes acute and chronic wound healing.It has unique surface activity, and in the time contacting with soft tissue wound surface, material moment can ion exchange occur with tissue, improve local partial pressure of oxygen and pH value, can promote emiocytosis collagen protein and the various wound healing factor, improve wound surface nutrient environment and microcirculation, promote granulation tissue growth.Staphylococcus aureus and escherichia coli etc. are had to significant inhibitory action, and can effectively prevent the infection of wound surface.The carbonated hydroxyapatite rack that surface forms, again as the support of cambium, makes cambium be climbed and move smoothly, thereby accelerates the healing of wound surface, shortens wound healing time, and particularly those lack the chronic wound of self-healing ability.Concrete material have that [Na2O-CaO-SiO2-P2O5] system forms micrometer level porous solid particle, there is high specific surface area, be conducive to its degraded in organizational environment, and avoid producing and do not meet the immunne response needing.This material is degraded to rich SiO in organizational environment
2gel layer, and form calcium hydroxy phosphate structure on its surface ,-Si-OH-and different types of protein can form highdensity protein adsorption by hydrogen bond and ion amine key (Si-O-H3N+-) combination, have fabulous biological activity.In organizational environment, make local location Ca+, Si+ concentration raises, and improves pH value, has certain broad-spectrum antibacterial action.The transudate of organizing to skin wound has certain Absorption, can make wound keep the dry of appropriateness.
Be badly in need of a kind of biomaterial with natural bacteriostatic function for protecting the medical material of wound, promote the micron order inorganic powder of wound healing at its surface adhesion, sticked behind wound surface, infiltrated by wound tissue fluid, enter wound tissue, promote wound healing, reduce cicatrix, and can suppress wound bacterial growth, also greatly facilitate clinical use simultaneously
Summary of the invention
The object of the invention is to overcome the deficiency of existing product, provide a kind of composite biological material to apply ointment or plaster, this biological material application has good biocompatibility, as all applicable in burn, scald and operative incision to various wounds, in promoting that organization healing and wound are repaired, reduce cicatrix, and there is the effect that suppresses wound surface bacterial growth, and there is good pliability, dilatancy and permeability.
Composite biological material of the present invention is applied ointment or plaster by base layer, protect wound layer and two peel ply compositions, protecting wound layer fits tightly in the center of base layer one side, after one end of two peel plies partly overlaps, cover and protect on wound layer, work to protecting wound layer the effect of protecting of covering, the two peel plies other end and base layer two port is separately neat and bonding, time easy to use, peel off, base layer with protect the side surface that closes of wound laminating and there is the ripple type on-woven medical air-permeable adhesive plaster that carries glue-line, protect wound layer for composite biological material, compound bio material is by expecting silk fibroin porous biomaterial, chitosan, micron order inorganic powder grains makes, peel ply is release paper, as Ge Laxin paper.
In composite biological material of the present invention, silk fibroin porous material thickness is 3-5mm, and every square metre of content of chitosan is 30-50g, and micron order inorganic powder grains content is every square metre and is not less than 30g.Wherein micron order inorganic powder grains is made up of [Na2O-CaO-SiO2-P2O5] system, and main component is SiO245%, Na2O24.5%, CaO24.5%, P2O56%, uniform grading 15 μ m ± 5 μ m.
Another object of the present invention is to provide described composite biological material and applies ointment or plaster and protect the preparation method of wound layer in product, realizes by following key step:
(1) give birth to natural silk degumming: configuration 1% suds washing liquid, it contains mass concentration is 0.5% NaHCO
3, will give birth to Silk Soaking wherein, silkworm silk is with solution weight than 1:25, and the temperature of immersion is 70 DEG C-90 DEG C, and the time of immersion is 40-60 minute, washed with de-ionized water, 70 DEG C-90 DEG C of water temperatures, constant temperature soaks 40-60 minute, loose wire, room temperature is air-dry, with for subsequent use;
(2) silk fibroin porous biomaterial preparation: step (1) is processed to the material obtaining through thick shredding; Speed is 0.20-0.40Hz feeding transmission means mixed cotton; Speed is the shredding of 0.20-0.40Hz essence; Shift by the vacuum transfer case containing dosing pump, the condition of controlling vacuum transfer case is: main cylinder speed: 20-30Hz, doffer speed: 20-30Hz, mixed and disorderly speed: 14-19Hz; Double-deck combing, carding condition is to be 0.2-0.4Hz to cotton speed, coaster speed is 8-10Hz; Through bilayer or multilamellar, to intersect or lay-up lapping, the oblique curtain speed of lapping is 10.0-15.0Hz, thickness is 3-5mm; Shift and carry, shifting end curtain output speed in conveying is 0.80-1.50Hz; Water thorn, water thorn mesh version version is thick is 0.8mm, the pressure of prewetting is 20-30bar, main thorn pressure is 25-35bar, and a counter-pressure is 35-45bar, and two counter-pressures are 40-50bar, three counter-pressures are 45-55bar, traction condition is: drawing-off 1 is closed as 2-4Hz, and drawing-off 2 is closed as 2-4Hz, and drawing-off 3 is closed as 2-4Hz; Vacuum drying, pressure is 25-50 atmospheric pressure; Rough lumber limit, through reel system tumbler carry, fritter;
(3) chitosan film preparation: the chitosan that is 8-40 ten thousand, deacetylation >90% by molecular weight is dissolved in acetic acid deionized water, and making quality is 0.5-1.5% chitosan solution than concentration; Chitosan solution is sprayed on the porous biomaterial that step (2) obtains, the consumption of chitosan solution for every square metre of content after solidifying be 30-50g;
(4) composite biological material preparation: micron order inorganic powder material is sprayed on the chitosan film that step (3) obtains, and consumption is that every square metre of chitosan film sprays and is not less than 30g, lyophilization;
(5) composite biological material step (4) being obtained specification cutting on request, to create layer, the finished product of applying ointment or plaster of making for subsequent use as protecting.
By nonwoven medical air-permeable adhesive plaster, step (5) obtain protecting wound layer, peel ply is positioned on the material shelf of composite roll from top to bottom successively, wherein protect wound and be placed on nonwoven Breathable adhesive tape center, two peel plies have fraction overlapping, and three carries out compound, make the composite biological material finished product of applying ointment or plaster.
The present invention selects specific method to make silk fibroin porous biomaterial, and chitosan, micron order inorganic powder are sprayed wherein according to a certain percentage, the silk fibroin porous biomaterial that the method that the present invention adopts makes, has stronger bacteriostasis, good biocompatibility.
The present invention has: (1) belongs to the natural biologic material wound product of applying ointment or plaster, and has good safety and biocompatibility, is applicable to various wounds; (2) effectively anti-inflammation sterilization, hemostasis, promote granulation tissue and epithelial cell growth, and wound healing and reparation are fast; (3) permeability is good, sticks softly, provides a favourable growing environment to wound surface.(4) group technology of the shredding-combing-lapping-water thorn adopting, according to preferred technological parameter, compared with the fibroin albumen that the silk fibroin porous biomaterial making and common process make as electrospinning process, has stronger bacteriostasis.On the porous biomaterial making, spray the micron order inorganic powder of biomaterial chitosan and promotion wound healing by the inventive method, the composite that makes three's composite integrated is applied ointment or plaster.
Brief description of the drawings
Fig. 1 is a kind of structural representation that composite biological material of the present invention is applied ointment or plaster.
Fig. 2 is silk fibroin porous biomaterial electronic scanner microscope (SEM) structural characterization prepared by embodiment 2-4.
Fig. 3 is the compound rear material electronics scanning microscope structural characterization of the silk fibroin porous biomaterial/chitosan of embodiment 2-4.
Fig. 4 is embodiment 2-4 silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite electronic scanner microscope structural characterization.
Fig. 5 is sample biocompatibility test electronic scanner microscope displayed map.
Fig. 6 is In Vitro Bacteriostasis experiment.
Detailed description of the invention
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
Referring to Fig. 1, a kind of medical sanitary protects wound and applies ointment or plaster, by base layer 1, protecting wound layer 2 and two peel plies 3 forms, protecting wound layer 2 fits tightly in the center of base layer 1 one sides, one end of two peel plies 3 partly overlaps to cover and protects on wound layer 2, work to protecting wound layer the effect of protecting of covering, two peel plies 3 other end and base layer 1 two ends are separately bonding, time easy to use, peel off, base layer 1 has with a side surface that protects 2 laminating of wound layer the ripple type on-woven medical air-permeable adhesive plaster that carries glue-line, protect the composite biological material of wound layer 2 for adopting the inventive method to prepare, this composite biological material comprises silk fibroin porous biomaterial, chitosan, micron order inorganic powder grains.Peel ply 3 is release paper, as Ge Laxin paper.
Embodiment 2 protects wound layer preparation method
(1) give birth to natural silk degumming: configuration 1% suds washing liquid, it contains mass concentration is 0.5% NaHCO
3, will give birth to Silk Soaking wherein, silkworm silk is with solution weight than 1:25, and the temperature of immersion is 70 DEG C, and the time of immersion is 40 minutes, washed with de-ionized water, 70 DEG C of water temperatures, constant temperature soaks 40 minutes, loose wire, room temperature is air-dry, with for subsequent use;
(2) silk fibroin porous biomaterial preparation: step (1) is processed to the material obtaining through thick shredding; Speed is 0.20Hz feeding transmission means mixed cotton; Speed is the shredding of 0.20Hz essence; Shift by the vacuum transfer case containing dosing pump, the condition of controlling vacuum transfer case is: main cylinder speed is 20Hz, and doffer speed is 20Hz, and mixed and disorderly speed is 14Hz; Double-deck combing, carding condition is to be 0.2Hz to cotton speed, coaster speed is 8Hz; Through bilayer, cross lapping, the oblique curtain speed of lapping is 10.0Hz, thickness is 3mm; Shift and carry, shifting end curtain output speed in conveying is 0.80Hz; Water thorn, water thorn mesh version version is thick is 0.8mm, and the pressure of prewetting is 20bar, and main thorn pressure is 25bar, and a counter-pressure is 35bar, and two counter-pressures are 40bar, and three counter-pressures are 45bar, and traction condition is: drawing-off 1 is closed as 2Hz, and drawing-off 2 is closed as 2Hz, and drawing-off 3 is closed as 2Hz; Vacuum drying, pressure is 25 atmospheric pressure; Rough lumber limit, through reel system tumbler carry, fritter;
The how biological hole of the fibroin albumen material making adopts electron scanning (SEM) microscope figure to carry out structural characterization, as the A in Fig. 2..
(3) chitosan film preparation: the chitosan that is 8-40 ten thousand, deacetylation >90% by molecular weight is dissolved in acetic acid deionized water, and making quality is 0.5% chitosan solution than concentration; Chitosan solution is sprayed on the porous biomaterial that step (2) obtains, the consumption of chitosan solution for every square metre of content after solidifying be 30g;
The compound rear material electronics scanning microscope structural characterization of silk fibroin porous biomaterial/chitosan, as Fig. 3 A;
(4) composite biological material preparation: micron order inorganic powder material is sprayed on the chitosan film that step (3) obtains, and consumption is that every square metre of chitosan film sprays and is not less than 30g, lyophilization;
Silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite electronic scanner microscope structural characterization, as Fig. 4 A;
(5) step (4) is obtained to composite biological material specification cutting on request, as protecting wound layer, the finished product of applying ointment or plaster of making for subsequent use.
Embodiment 3 protects wound layer preparation technology
(1) give birth to natural silk degumming: configuration 1% suds washing liquid, it contains mass concentration is 0.5% NaHCO
3, will give birth to Silk Soaking wherein, silkworm silk is with solution weight than 1:25, and the temperature of immersion is 90 DEG C, and the time of immersion is 60 minutes, washed with de-ionized water, 90 DEG C of water temperatures, constant temperature soaks 60 minutes, loose wire, room temperature is air-dry, with for subsequent use;
(2) silk fibroin porous biomaterial preparation: step (1) is processed to the material obtaining through thick shredding; Speed is 0.40Hz feeding transmission means mixed cotton; Speed is the shredding of 0.40Hz essence; Shift by the vacuum transfer case containing dosing pump, the condition of controlling vacuum transfer case is: main cylinder speed is 30Hz, and doffer speed is 30Hz, and mixed and disorderly speed is 19Hz; Double-deck combing, carding condition is to be 0.4Hz to cotton speed, coaster speed is 10Hz; Through bilayer or multilamellar, to intersect or lay-up lapping, the oblique curtain speed of lapping is 15.0Hz, thickness is 5mm; Shift and carry, shifting end curtain output speed in conveying is 1.50Hz; Water thorn, water thorn mesh version version is thick is 0.8mm, and the pressure of prewetting is 30bar, and main thorn pressure is 35bar, and a counter-pressure is 45bar, and two counter-pressures are 50bar, and three counter-pressures are 55bar, and traction condition is: drawing-off 1 is closed as 4Hz, and drawing-off 2 is closed as 4Hz, and drawing-off 3 is closed as 4Hz; Vacuum drying, pressure is 50 atmospheric pressure; Rough lumber limit, through reel system tumbler carry, fritter;
The silk fibroin porous biomaterial making adopts electron scanning (SEM) microscope figure to carry out structural characterization, as the B in Fig. 2;
(3) chitosan film preparation: the chitosan that is 8-40 ten thousand, deacetylation >90% by molecular weight is dissolved in acetic acid deionized water, and making quality is 0.5-1.5% chitosan solution than concentration; Chitosan solution is sprayed on the porous biomaterial that step (2) obtains, the consumption of chitosan solution for every square metre of content after solidifying be 30-50g;
The compound rear material electronics scanning microscope structural characterization of silk fibroin porous biomaterial/chitosan, as Fig. 3 B;
(4) composite biological material preparation: micron order inorganic powder material is sprayed on the chitosan film that step (3) obtains, and consumption is that every square metre of chitosan film sprays and is not less than 30g, lyophilization;
Silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite electronic scanner microscope structural characterization, as Fig. 4 B;
(5) step (4) is obtained to composite biological material specification cutting on request, as protecting wound layer, the finished product of applying ointment or plaster of making for subsequent use.
Embodiment 4 protects wound layer preparation method
(1) give birth to natural silk degumming: configuration 1% suds washing liquid, it contains mass concentration is 0.5% NaHCO
3, will give birth to Silk Soaking wherein, the temperature of immersion is 80 DEG C, the time of immersion is 50 minutes, washed with de-ionized water, 80 DEG C of water temperatures, constant temperature soaks 50 minutes, loose wire, room temperature is air-dry, with for subsequent use;
(2) silk fibroin porous biomaterial preparation: step (1) is processed to the material obtaining through thick shredding; Speed is 0.30Hz essence feeding transmission means mixed cotton; Speed is the shredding of 0.30Hz essence; Shift by the vacuum transfer case containing dosing pump, the condition of controlling vacuum transfer case is: main cylinder speed is 25Hz, and doffer speed is 25Hz, and mixed and disorderly speed is 17Hz; Double-deck combing, carding condition is to be 0.3Hz to cotton speed, coaster speed is 9.0Hz; Through bilayer, lay-up lapping, the oblique curtain speed of lapping is 13.0Hz, thickness is 4mm; Shift and carry, shifting end curtain output speed in conveying is 1.20Hz; Water thorn, water thorn mesh version version is thick is 0.8mm, and the pressure of prewetting is 25bar, and main thorn pressure is 30bar, and a counter-pressure is 40bar, and two counter-pressures are 45bar, and three counter-pressures are 50bar, and traction condition is: drawing-off 1 is closed as 3Hz, and drawing-off 2 is closed as 3Hz, and drawing-off 3 is closed as 3Hz; Vacuum drying, pressure is 35 atmospheric pressure; Rough lumber limit, through reel system tumbler carry, fritter;
The silk fibroin porous biomaterial making adopts electron scanning (SEM) microscope figure to carry out structural characterization, as the C in Fig. 2;
(3) chitosan film preparation: the chitosan that is 8-40 ten thousand, deacetylation >90% by molecular weight is dissolved in acetic acid deionized water, and making quality is 0.5-1.5% chitosan solution than concentration; Chitosan solution is sprayed on the porous biomaterial that step (2) obtains, the consumption of chitosan solution for every square metre of content after solidifying be 40g;
The compound rear material electronics scanning microscope structural characterization of silk fibroin porous biomaterial/chitosan, as Fig. 3 C;
(4) composite biological material preparation: micron order inorganic powder material is sprayed on the chitosan film that step (3) obtains, and consumption is that every square metre of chitosan film sprays and is not less than 30g, lyophilization;
Silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite electronic scanner microscope structural characterization, as Fig. 4 C;
(5) composite biological material step (4) being obtained is by specification cutting, as protecting wound layer, the finished product of applying ointment or plaster of making for subsequent use.
The preparation of applying ointment or plaster of embodiment 5 composite biological materials
By nonwoven medical air-permeable adhesive plaster, embodiment 2,3,4 steps (5) obtain protecting wound layer, two peel plies are positioned on the material shelf of composite roll from top to bottom successively, wherein protect wound and be placed on nonwoven Breathable adhesive tape center, two peel plies have fraction overlapping, three carries out compound, makes the composite biological material finished product of applying ointment or plaster.
Embodiment 6 biocompatibility experiments
Choose the composite biological material making sample that embodiment 2,3,4 makes, carry out biocompatibility test.
Scanning electron microscope example preparation method: culture dish is chosen cell title: NCTC clone 929(L929 l cell), purchased from Chinese Typical Representative culture collection center, numbering: GDC034.Sample is fixed after 12 hours with 2.5% glutaraldehyde; Wash two hours above (centre is changed new liquid 3 times) with 0.1mol/L phosphate buffer; Fix 1.5 hours through 1% osmic acid again; Wash 2 hours (centre is changed 3 times) with DDW; Then through 50%, 70%, 80%, 90%, 100%(2 time) ethanol dehydration, every grade 15 minutes; Isoamyl acetate displacement 30 minutes; HCP-2 critical point. drying instrument is dry, platinum plating in IB-5 ion sputtering instrument after sticky holder, the S----4800 of Hitachi sem observation is also taken pictures.
Result referring to Fig. 5: SEM show L929 in three samples well-grown and have pseudopodium generate, show that composite biological material of the present invention has good biocompatibility.
Embodiment 7 zooperies
Choose 16 of male SD rats, rat body weight is 300-400 gram, 10% chloral hydrate intraperitoneal anesthesia, 100mgX0.33ml, select the gluteus maximus of vistar rats with bilateral buttocks, wound surface diameter >=1.2CM, excision skin, subcutaneous fascia and part gluteus maximus (to blood oozing from the wound surface shape), place experiment material, medical desensitization adhesive plaster wound closure.Postoperative single cage is fed.Wherein No. 1-8 left side sample is silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite, and right side is silk fibroin porous material/chitosan; 9-16 left side is micron inorganic powder grains, and right side is silk fibroin porous material/micron inorganic powder grains.Wound Contraction rate observed result is referring to table 1.
Table 1
Zoopery is carried out to overall merit, and knot is referring to table 2.
Table 2
Note: "+" expression for good result degree, "+" more multilist shows that effect is better.
Conclusion: silk fibroin porous biomaterial/chitosan/micron inorganic powder grains composite aspect hemostasis, wound healing, minimizing cicatrix than single component or two component better effects if wherein.
Embodiment 8 In Vitro Bacteriostasis experiments
The composite that fibroin albumen+chitosan+micron inorganic powder grains that specific method of the present invention makes is made, the silk fibroin porous material making with conventional process, fibroin albumen+Chitosan Composites that conventional process makes, contrast its fungistatic effect.
The silk fibroin porous material common process here for reported electrospinning process ([academic dissertation 11] Hu Aijun. the experimentation [D] of regeneration Static Spinning fibroin nanofiber effect in nervus facialis is repaired. Suzhou: University Of Suzhou, 2012:23.) fibroin albumen that makes; Fibroin albumen+Chitosan Composites common process is here according to paper: [academic dissertation 11] Cai Zengxiao. fibroin albumen/chitosan composite nano fibre is for skin tissue engineering [D]. and Shanghai: Donghua University, 2011:22-35.
Concrete operations: get culture dish, inoculate respectively escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, Candida albicans with dull and stereotyped coating method, then get respectively the silk fibroin porous material that conventional process makes, fibroin albumen+Chitosan Composites that conventional process makes, and the composite biological material of embodiment 2,3,4 preparations, be cut to the sample of diameter 5mm, be placed on culture dish, cultivate after 24 lab scales, observe inhibition zone.
In body outer suppressioning experiment, microscopic examination result shows, compared with matched group, composite biological material prepared by the present invention is to escherichia coli, staphylococcus aureus, pseudomonas aeruginosa has very significantly better fungistatic effect, to Candida albicans inhibition no significant difference, result is referring to Fig. 6, in Fig. 6, A is the antibacterial result of silk fibroin porous material prepared by conventional method, E is the antibacterial result of fibroin albumen+Chitosan Composites that conventional process makes, B, C, D is the embodiment of the present invention 2, 3, 4 prepare the antibacterial result of sample, wherein 1 is escherichia coli culture dishs, the 2nd, staphylococcus aureus culture dish, the 3rd, pseudomonas aeruginosa culture dish, the 4th, Candida albicans culture dish.
Above embodiment is the specific embodiment of the present invention, is to further detailed description of the present invention, can not assert that embodiments of the present invention only limit to this.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (3)
1. a composite biological material is applied ointment or plaster, by base layer (1), protect wound layer (2) and two peel plies (3) composition, protecting wound layer (2) fits tightly in the center of base layer (1) one side, one end of two peel plies (3) partly overlaps to cover and protects on wound layer (2), two peel plies (3) other end and base layer (1) two end is separately bonding, time easy to use, peel off, base layer (1) has with a side surface that protects wound layer (2) laminating the ripple type on-woven medical air-permeable adhesive plaster that carries glue-line, peel ply (3) is release paper, it is characterized in that, protect wound layer (2) for composite biological material, described composite biological material is by silk fibroin porous biomaterial, chitosan, micron order inorganic powder grains makes.
2. a kind of composite biological material according to claim 1 is applied ointment or plaster, it is characterized in that, in described composite biological material, silk fibroin porous material thickness is 3-5mm, every square metre of content of chitosan is 30-50g, and micron order inorganic powder grains content is every square metre and is not less than 30g, and wherein micron order inorganic powder grains is made up of [Na2O-CaO-SiO2-P2O5] system, main component is SiO2 45%, Na2O 24.5%, CaO 24.5%, P2O5 6%, uniform grading 15 μ m ± 5 μ m.
3. a kind of composite biological material according to claim 1 is applied ointment or plaster, and it is characterized in that, described composite biological material is prepared by following steps:
(1) give birth to natural silk degumming: configuration 1% suds washing liquid, it contains mass concentration is 0.5% NaHCO
3, will give birth to Silk Soaking wherein, silkworm silk is with solution weight than 1:25, and the temperature of immersion is 70 DEG C-90 DEG C, and the time of immersion is 40-60 minute, washed with de-ionized water, 70 DEG C-90 DEG C of water temperatures, constant temperature soaks 40-60 minute, loose wire, room temperature is air-dry, with for subsequent use;
(2) silk fibroin porous biomaterial preparation: step (1) is processed to the material obtaining through thick shredding; Speed is 0.20-0.40Hz feeding transmission means mixed cotton; Speed is the shredding of 0.20-0.40Hz essence; Shift by the vacuum transfer case containing dosing pump, the condition of controlling vacuum transfer case is: main cylinder speed: 20-30Hz, doffer speed: 20-30Hz, mixed and disorderly speed: 14-19 Hz; Double-deck combing, carding condition is to be 0.2-0.4 Hz to cotton speed, coaster speed is 8-10Hz; Through bilayer or multilamellar, to intersect or lay-up lapping, the oblique curtain speed of lapping is 10.0-15.0Hz, thickness is 3-5mm; Shift and carry, shifting end curtain output speed in conveying is 0.80-1.50Hz; Water thorn, water thorn mesh version version is thick is 0.8mm, the pressure of prewetting is 20-30bar, main thorn pressure is 25-35bar, and a counter-pressure is 35-45bar, and two counter-pressures are 40-50 bar, three counter-pressures are 45-55 bar, traction condition is: drawing-off 1 is closed as 2-4 Hz, and drawing-off 2 is closed as 2-4Hz, and drawing-off 3 is closed as 2-4Hz; Vacuum drying, pressure is 25-50 atmospheric pressure; Rough lumber limit, through reel system tumbler carry, fritter;
(3) chitosan film preparation: the chitosan that is 8-40 ten thousand, deacetylation >90% by molecular weight is dissolved in acetic acid deionized water, and making quality is 0.5-1.5% chitosan solution than concentration; Chitosan solution is sprayed on the porous biomaterial that step (2) obtains, the consumption of chitosan solution for every square metre of content after solidifying be 30-50g;
(4) composite biological material preparation: micron order inorganic powder material is sprayed on the chitosan film that step (3) obtains, and consumption is that every square metre of chitosan film sprays and is not less than 30g, lyophilization;
(5) composite biological material step (4) being obtained specification cutting on request, as protecting wound layer, makes the finished product of applying ointment or plaster.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410124365.5A CN103908692B (en) | 2014-03-30 | 2014-03-30 | A kind of composite biological material is applied ointment or plaster and preparation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104383585A (en) * | 2014-11-29 | 2015-03-04 | 山东省立医院 | Three-dimensional nanocomposite adhesive bandage as well as preparation method and use method thereof |
| CN105544284B (en) * | 2015-12-15 | 2017-07-11 | 浙江理工大学 | A kind of medical silk paper and preparation method thereof |
| CN107899061A (en) * | 2017-11-13 | 2018-04-13 | 广东泰宝医疗科技股份有限公司 | A kind of alginates wound repair dressing and preparation method thereof |
| CN109044626A (en) * | 2018-06-28 | 2018-12-21 | 佛山市瑞生海特生物科技有限公司 | It is capable of the biological wound-protecting film of anti-inflammatory anti-itch |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104383585A (en) * | 2014-11-29 | 2015-03-04 | 山东省立医院 | Three-dimensional nanocomposite adhesive bandage as well as preparation method and use method thereof |
| CN105544284B (en) * | 2015-12-15 | 2017-07-11 | 浙江理工大学 | A kind of medical silk paper and preparation method thereof |
| CN107899061A (en) * | 2017-11-13 | 2018-04-13 | 广东泰宝医疗科技股份有限公司 | A kind of alginates wound repair dressing and preparation method thereof |
| CN109044626A (en) * | 2018-06-28 | 2018-12-21 | 佛山市瑞生海特生物科技有限公司 | It is capable of the biological wound-protecting film of anti-inflammatory anti-itch |
| CN109044626B (en) * | 2018-06-28 | 2021-08-31 | 鹤壁市人民医院 | Biological wound protection film capable of diminishing inflammation and relieving itching |
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| CN103908692B (en) | 2016-05-18 |
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