CN103852580A - Staphylococcus aureus identification kit and preparation method thereof - Google Patents
Staphylococcus aureus identification kit and preparation method thereof Download PDFInfo
- Publication number
- CN103852580A CN103852580A CN201410057836.5A CN201410057836A CN103852580A CN 103852580 A CN103852580 A CN 103852580A CN 201410057836 A CN201410057836 A CN 201410057836A CN 103852580 A CN103852580 A CN 103852580A
- Authority
- CN
- China
- Prior art keywords
- solution
- polystyrene microsphere
- preparation
- concentration
- damping fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/305—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F)
- G01N2333/31—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Micrococcaceae (F) from Staphylococcus (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention relates to a staphylococcus aureus identification kit and a preparation method thereof. The staphylococcus aureus identification kit comprises a detection reagent R1 and a negative control reagent R2, wherein the negative control reagent R2 is a mixture of polystyrene microspheres and a microsphere buffer solution. The kit is characterized in that the detection reagent R1 uses the mixture of the immune polystyrene microspheres and the microsphere buffer solution; the immune polystyrene microspheres are formed by coupling hydroxyl functional polystyrene microspheres and at least two specific proteins. According to the preparation method of the hydroxyl functional polystyrene microspheres, alkylate chitosan reacts with the polystyrene microspheres; by using high-density hydroxyl distributed on the surface of the hydroxyl functional polystyrene microspheres, the coupling efficiency of the hydroxyl functional polystyrene microspheres and specific protein is improved, and the content of the specific protein or antibody substances coupled on the surface of the immune polystyrene microspheres is greatly increased. The staphylococcus aureus identification kit has the advantages of high detection sensitivity, high accuracy, low omission ratio, and the like.
Description
Technical field
The invention belongs to biotechnology and microorganism detection field, relate to a kind of staphylococcus aureus Rapid identification kit based on immune polystyrene microsphere agglutinating reaction.The present invention is applicable to the staphylococcus aureus fast detecting in Clinical microorganism or food microorganisms sample.
Technical background
Staphylococcus is the raw common bacterial strain of dwelling on human skin and mucous membrane.Wherein staphylococcus aureus can cause the pyogenic infection on a large scale including epidermis suppurates, food poisoning and toxic shock, and other various diseases.Therefore staphylococcus aureus is one of modal pathogenic bacteria of often running into of laboratory, because infection of staphylococcus aureus occurrence frequency is high and serious, and staphylococcus aureus have drug resistance to numerous conventional antibiosis, therefore in the urgent need to diagnosis fast and accurately, to patient is taked to the measure of effectively treating.In food manufacturing, find that in addition staphylococcus is the indicator bacteria of good microbial total body pollution level, therefore need a kind of staphylococcus aureus rapid identification method of high degree of specificity to infecting serious sample.
Latex agglutination is a kind of comparatively conventional method for quick occurring in recent years.The method is utilized the distinctive plasma-coagulase of staphylococcus aureus surface, the principle that can react with plasma fibrin material generation specific agglutination, plasma fibrin material and latex particle covalent coupling are prepared into the latex particle of sensitization, sensitization microballoon after this is crosslinked can react with the plasma-coagulase of above-mentioned staphylococcus aureus surface, produce microballoon agglutination phenomenon, thereby reach the object of Rapid identification staphylococcus aureus.The method has higher specificity, and detection time is short, and detection efficiency is high.But, the minority staphylococcus aureus strains that presents Coagulase-negative Staphylococci is had to undetected phenomenon, cause false-negative result, accuracy in detection is poor.
Summary of the invention
Technical matters to be solved by this invention is to provide staphylococcus aureus identification kit that a kind of detection speed is fast, detection sensitivity is high, accuracy in detection is good and preparation method thereof.
The technical scheme of staphylococcus aureus identification kit of the present invention comprises detection reagent R1 and negative control reagent R2, negative control reagent R2 is the potpourri of polystyrene microsphere and microballoon damping fluid, it is characterized in that detecting reagent R1 and adopt the potpourri of immune polystyrene microsphere and microballoon damping fluid, immune polystyrene microsphere adopts hydroxyl functional polystyrene microsphere and at least two kinds of differential protein couplings to form.
Preparation method of the present invention is:
1, detect the preparation method of reagent R1: in every 100ml microballoon damping fluid, add 0.5g immunity polystyrene microsphere;
2, the preparation method of negative control reagent R2: in every 100ml microballoon damping fluid, add 0.5g polystyrene microsphere;
Wherein the preparation method of microballoon damping fluid is:
1.. compound concentration is 0.1～1M, phosphate buffer, tri s damping fluid, carbonate buffer solution, glycine buffer that pH value is 6.0～9.0;
2.. get above-mentioned a kind of damping fluid, add one or more compounds in Qu Latong series and tween series, making the compound concentration after preparation is 0.5～2%;
3.. in the damping fluid preparing in 2. to step, add one or more compounds in PEG2000, casein, gelatin, skim milk, making the compound concentration after preparation is 1～5%;
4.. the microballoon damping fluid that 3. step is prepared stirs, and the temperature environment that is placed in 2～8 ℃ is preserved;
It is characterized in that:
The preparation method of immunity polystyrene microsphere is:
1.. hydroxyl functional polystyrene microsphere is scattered in aqueous solution, and making its concentration is 2～5%;
2.. get above-mentioned solution 10ml, add the NaIO4 solution that concentration that 1～2ml newly joins is 0.1～0.5M/L, mix, 4 ℃ of temperature environments leave standstill 20 minutes;
3.. 2. in solution, add 1～2ml in step, the glycol water of 0.16M, mixes, and room temperature lucifuge leaves standstill 30～60 minutes;
4.. in the solution of 3. obtaining in step, add differential protein solution, mix, pack bag filter into, slowly stir with 0.05M/LPH7.0 carbonate buffer solution, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway, after mixing, differential protein concentration is 1～10mg/ml;
5.. in the solution of 4. obtaining in step, adding freshly prepared concentration is NaBH4 solution 2～5ml of 5mg/ml, mix, in 4 ℃ of temperature environments, react after 2 hours, pack bag filter into, slowly stir with 0.1M/LPH7.0 phosphate buffer, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway;
6.. take out the reactant liquor of dialyse, add to 20ml with 0.1M/LPH7.0PBS, be placed in the temperature environment preservation of 2～8 ℃.
Preferably, the preparation method of hydroxyl functional polystyrene microsphere is:
1.. alkylated chitosan: shitosan, KOH, isopropyl alcohol are mixed and be placed in three-neck flask, at the uniform velocity stir, be warming up to 40 ℃ and keep 1 hour, make to be warming up to 60 ℃ after shitosan alkalization, and drip halogenated hydrocarbons, constant temperature stirring reaction 2～12 hours; Wherein every ml isopropyl alcohol adds 0.05～0.1g shitosan and 0.1～0.2g KOH; Every ml reactant liquor is containing 0.10～0.30ml halogenated hydrocarbons;
2.. by through acetone and alternately washing of distilled water for step reactant 1., until no longer include halogen ion in the distilled water washing out, after oven dry, obtain alkylated chitosan;
3.. the alkylated chitosan that 2. step is obtained, being dissolved in concentration is in 1～5% acetum, leaves standstill 16～20 hours, makes it fully dissolve dispersion, after dissolving, the concentration of alkylated chitosan is 1～5%;
4.. the step of learning from else's experience alkylated chitosan solution 3., be added drop-wise in polystyrene microsphere solution, under room temperature, at the uniform velocity stir 1～10 hour, be after homodisperse colloidal solution, centrifuging obtains hydroxyl functional polystyrene microsphere; In hydroxyl functional polystyrene microsphere solution, the concentration of alkylated chitosan is 0.01～0.5%, and the concentration of polystyrene microsphere is 0.1～1%.
One of advantage of the present invention is: a kind of new staphylococcus aureus rapid identification method based on immune polystyrene microsphere agglutinating reaction is provided, the method adopts Multiple detection mechanism, identify plasma-coagulase, SPA, polysaccharide antigen, capsular antigen of staphylococcus aureus surface etc. simultaneously, improve the specificity and the accuracy that detect reagent, reduced loss;
Two of advantage of the present invention is: a kind of hydroxyl functional polystyrene microsphere preparation method is provided, adopt alkylated chitosan to react with polystyrene microsphere, being prepared into a kind of surface distributed has the functional polystyrene microballoon of high density oh group, adopt hydroxyl functional polystyrene microsphere and antibody materials coupling to be prepared into immune polystyrene microsphere, the high density hydroxyl that utilizes hydroxyl functional Surfaces of Polystyrene Microparticles to distribute, improve the coupling efficiency of hydroxyl functional polystyrene microsphere and differential protein, differential protein or antibody materials in immune Surfaces of Polystyrene Microparticles coupling are improved greatly, improve the detection sensitivity of reagent.
Embodiment
Staphylococcus aureus identification kit of the present invention, comprise and detect reagent R1 and negative control reagent R2, negative control reagent R2 is the potpourri of polystyrene microsphere and microballoon damping fluid, detecting reagent R1 is the potpourri of immune polystyrene microsphere and microballoon damping fluid, and immune polystyrene microsphere adopts hydroxyl functional polystyrene microsphere and at least two kinds of differential protein couplings to form.Described differential protein can be human or animal's blood plasma, human or animal's IgG immunoglobulin (Ig), anti-SPA monoclonal antibody or polyclonal antibody, anti-Staphylococcus aureus capsular polysaccharide antigen monoclonal antibody or polyclonal antibody, anti-Staphylococcus aureus monoclonal antibody or polyclonal antibody.
The compound method of staphylococcus aureus identification kit of the present invention:
1, detect the preparation method of reagent R1: in every 100ml microballoon damping fluid, add 0.5g immunity polystyrene microsphere;
2, the preparation method of negative control reagent R2: in every 100ml microballoon damping fluid, add 0.5g polystyrene microsphere;
Wherein the preparation method of microballoon damping fluid is:
1.. compound concentration is 0.1～1M, phosphate buffer, tr i s damping fluid, carbonate buffer solution, glycine buffer that pH value is 6.0～9.0;
2.. get above-mentioned a kind of damping fluid, add one or more compounds in Qu Latong series and tween series, making the compound concentration after preparation is 0.5～2%;
3.. in the damping fluid preparing in 2. to step, add one or more compounds in PEG2000, casein, gelatin, skim milk, making the compound concentration after preparation is 1～5%;
4.. 2. step is stirred with the microballoon damping fluid that 3. step prepares, and the temperature environment that is placed in 2～8 ℃ is preserved;
The preparation method of immunity polystyrene microsphere is:
1.. hydroxyl functional polystyrene microsphere is scattered in aqueous solution, and making its concentration is 2～5%;
2.. get above-mentioned solution 10ml, add the NaIO4 solution that concentration that 1～2ml newly joins is 0.1～0.5M/L, mix, 4 ℃ of temperature environments leave standstill 20 minutes;
3.. 2. in solution, add 1～2ml in step, the glycol water of 0.16M, mixes, and room temperature lucifuge leaves standstill 30～60 minutes;
4.. in the solution of 3. obtaining in step, add differential protein solution, mix, pack bag filter into, slowly stir with 0.05M/LPH7.0 carbonate buffer solution, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway, after mixing, differential protein concentration is 1～10mg/ml;
5.. in the solution of 4. obtaining in step, adding freshly prepared concentration is NaBH4 solution 2～5ml of 5mg/ml, mix, in 4 ℃ of temperature environments, react after 2 hours, pack bag filter into, slowly stir with 0.1M/LPH7.0 phosphate buffer, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway;
6.. take out the reactant liquor of dialyse, add to 20ml with 0.1M/LPH7.0PBS, be placed in the temperature environment preservation of 2～8 ℃.
The preparation method of hydroxyl functional polystyrene microsphere is:
1.. alkylated chitosan: shitosan, KOH, isopropyl alcohol are mixed and be placed in three-neck flask, at the uniform velocity stir, be warming up to 40 ℃ and keep 1 hour, make to be warming up to 60 ℃ after shitosan alkalization, and drip halogenated hydrocarbons, constant temperature stirring reaction 2～12 hours; Wherein every ml isopropyl alcohol adds 0.05～0.1g shitosan and 0.1～0.2g KOH; Every ml reactant liquor is containing 0.10～0.30ml halogenated hydrocarbons;
2.. by through acetone and alternately washing of distilled water for step reactant 1., until no longer include halogen ion in the distilled water washing out, after oven dry, obtain alkylated chitosan;
3.. the alkylated chitosan that 2. step is obtained, being dissolved in concentration is in 1～5% acetum, leaves standstill 16～20 hours, makes it fully dissolve dispersion, after dissolving, the concentration of alkylated chitosan is 1～5%;
4.. the step of learning from else's experience alkylated chitosan solution 3., be added drop-wise in polystyrene microsphere solution, under room temperature, at the uniform velocity stir 1～10 hour, be after homodisperse colloidal solution, centrifuging obtains hydroxyl functional polystyrene microsphere; In hydroxyl functional polystyrene microsphere solution, the concentration of alkylated chitosan is 0.01～0.5%, and the concentration of polystyrene microsphere is 0.1～1%.
Preferred version prepared by hydroxyl functional polystyrene microsphere is:
1.. the alkylation of shitosan: 5g shitosan, 10g KOH are mixed and are placed in three-neck flask with 100ml isopropyl alcohol, at the uniform velocity stir, be warming up to 40 ℃ and keep 1 hour, make to be warming up to 60 ℃ after shitosan alkalization, and drip 20ml halogenated hydrocarbons, constant temperature stirring reaction 8 hours; Halogenated hydrocarbons adopts chlorinated dodecane, and the alkylated chitosan being prepared into is dodecyl shitosan;
2.. by through acetone and alternately washing of distilled water for step reactant 1., until no longer include halogen ion in the distilled water washing out, after oven dry, obtain alkylated chitosan;
3.. by alkylated chitosan, be dissolved in concentration and be in 2% acetum, leave standstill 12 hours, make it fully dissolve dispersion, after dissolving, the concentration of alkylated chitosan is 1%;
4.. the step of learning from else's experience alkylated chitosan solution 3., be added drop-wise in polystyrene microsphere solution, the concentration that makes alkylated chitosan is 0.01%, the concentration of polystyrene microsphere is 0.5%, under room temperature, at the uniform velocity stir 5 hours, be after homodisperse colloidal solution, centrifuging obtains the polystyrene microsphere of hydroxyl functional.
Preferred version prepared by microballoon damping fluid is:
1.. compound concentration is 1M, the phosphate buffer that pH value is 6.0;
2.. in above-mentioned phosphate buffer, add 1% Qu Latong and 0.5% tween 10, stir;
3.. in the damping fluid preparing in 2. to step, add 1% PEG2000,2% casein, stir;
4.. 2. step is stirred with the cocktail buffer that 3. step prepares, and by make microballoon damping fluid be placed in 4 ℃ temperature environment preserve.
Preferred version prepared by immunity polystyrene microsphere is:
1.. hydroxyl functional polystyrene microsphere is scattered in aqueous solution, and making its final concentration is 3%;
2.. get above-mentioned solution 10ml, add the NaIO4 solution that concentration that 2ml newly joins is 0.5M/L, mix, 4 ℃ of temperature environments leave standstill 20 minutes;
3.. 2. add the glycol water of 2ml, 0.16M in solution in step, mix, room temperature lucifuge leaves standstill 30 minutes;
4.. in the solution of 3. obtaining in step, add human plasma, anti-SPA monoclonal antibody, anti-Staphylococcus aureus capsular polysaccharide monoclonal antibody differential protein solution, the concentration that makes various differential proteins is 2mg/ml, after mixing, pack bag filter into, slowly stir with 0.05M/LPH7.0 carbonate buffer solution, in 4 ℃ of temperature environments, dialyse 12 hours, change secondary dislysate midway;
5.. in the solution of 4. obtaining in step, adding freshly prepared concentration is the NaBH4 solution 2ml of 5mg/ml, mix, in 4 ℃ of temperature environments, react after 2 hours, pack bag filter into, slowly stir with 0.1M/LPH7.0 phosphate buffer, in 4 ℃ of temperature environments, dialyse 12 hours, change secondary dislysate midway;
6.. take out the reactant liquor of dialyse, add to 20ml with 0.1M/LPH7.0PBS, put the temperature environment preservation of 4 ℃.
Claims (3)
1. a staphylococcus aureus identification kit, comprise and detect reagent R1 and negative control reagent R2, negative control reagent R2 is the potpourri of polystyrene microsphere and microballoon damping fluid, it is characterized in that detecting reagent R1 and adopt the potpourri of immune polystyrene microsphere and microballoon damping fluid, immune polystyrene microsphere adopts hydroxyl functional polystyrene microsphere and at least two kinds of differential protein couplings to form.
2. the preparation method of staphylococcus aureus identification kit according to claim 1:
1), detect the preparation method of reagent R1: in every 100ml microballoon damping fluid, add 0.5g immunity polystyrene microsphere;
2), the preparation method of negative control reagent R2: in every 100ml microballoon damping fluid, add 0.5g polystyrene microsphere;
Wherein the preparation method of microballoon damping fluid is:
1.. compound concentration is 0.1～1M, phosphate buffer, tri s damping fluid, carbonate buffer solution, glycine buffer that pH value is 6.0～9.0;
2.. get above-mentioned a kind of damping fluid, add one or more compounds in Qu Latong series and tween series, making the compound concentration after preparation is 0.5～2%;
3.. in the damping fluid preparing in 2. to step, add one or more compounds in PEG2000, casein, gelatin, skim milk, making the compound concentration after preparation is 1～5%;
4.. the microballoon damping fluid that 3. step is prepared stirs, and the temperature environment that is placed in 2～8 ℃ is preserved;
It is characterized in that:
The preparation method of immunity polystyrene microsphere is:
1.. hydroxyl functional polystyrene microsphere is scattered in aqueous solution, and making its concentration is 2～5%;
2.. get above-mentioned solution 10ml, add the NaIO4 solution that concentration that 1～2ml newly joins is 0.1～0.5M/L, mix, 4 ℃ of temperature environments leave standstill 20 minutes;
3.. 2. in solution, add 1～2ml in step, the glycol water of 0.16M, mixes, and room temperature lucifuge leaves standstill 30～60 minutes;
4.. in the solution of 3. obtaining in step, add differential protein solution, mix, pack bag filter into, slowly stir with 0.05M/LPH7.0 carbonate buffer solution, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway, after mixing, differential protein concentration is 1～10mg/ml;
5.. in the solution of 4. obtaining in step, adding freshly prepared concentration is NaBH4 solution 2～5ml of 5mg/ml, mix, in 4 ℃ of temperature environments, react after 2 hours, pack bag filter into, slowly stir with 0.1M/LPH7.0 phosphate buffer, in 4 ℃ of temperature environments, dialyse 16～20 hours, change secondary dislysate midway;
6.. take out the reactant liquor of dialyse, add to 20ml with 0.1M/LPH7.0PBS, be placed in the temperature environment preservation of 2～8 ℃.
3. the preparation method of staphylococcus aureus identification kit according to claim 2, is characterized in that the preparation method of hydroxyl functional polystyrene microsphere is:
1.. alkylated chitosan: shitosan, KOH, isopropyl alcohol are mixed and be placed in three-neck flask, at the uniform velocity stir, be warming up to 40 ℃ and keep 1 hour, make to be warming up to 60 ℃ after shitosan alkalization, and drip halogenated hydrocarbons, constant temperature stirring reaction 2～12 hours; Wherein every ml isopropyl alcohol adds 0.05～0.1g shitosan and 0.1～0.2g KOH; Every ml reactant liquor is containing 0.10～0.30ml halogenated hydrocarbons;
2.. by through acetone and alternately washing of distilled water for step reactant 1., until no longer include halogen ion in the distilled water washing out, after oven dry, obtain alkylated chitosan;
3.. the alkylated chitosan that 2. step is obtained, being dissolved in concentration is in 1～5% acetum, leaves standstill 16～20 hours, makes it fully dissolve dispersion, after dissolving, the concentration of alkylated chitosan is 1～5%;
4.. the step of learning from else's experience alkylated chitosan solution 3., be added drop-wise in polystyrene microsphere solution, under room temperature, at the uniform velocity stir 1～10 hour, be after homodisperse colloidal solution, centrifuging obtains hydroxyl functional polystyrene microsphere; In hydroxyl functional polystyrene microsphere solution, the concentration of alkylated chitosan is 0.01～0.5%, and the concentration of polystyrene microsphere is 0.1～1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410057836.5A CN103852580B (en) | 2014-02-18 | 2014-02-18 | Staphylococcus aureus identification kit and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410057836.5A CN103852580B (en) | 2014-02-18 | 2014-02-18 | Staphylococcus aureus identification kit and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103852580A true CN103852580A (en) | 2014-06-11 |
| CN103852580B CN103852580B (en) | 2015-12-09 |
Family
ID=50860486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410057836.5A Active CN103852580B (en) | 2014-02-18 | 2014-02-18 | Staphylococcus aureus identification kit and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103852580B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603348A (en) * | 2004-09-14 | 2005-04-06 | 清华大学 | Process for synthesis of monodisperse micron crosslinked polystyrene copolymerization microsphere |
| US20050153370A1 (en) * | 2003-12-30 | 2005-07-14 | 3M Innovative Properties Company | Method of enhancing signal detection of cell-wall components of cells |
| WO2009039854A2 (en) * | 2007-09-27 | 2009-04-02 | Dako Denmark A/S | Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics |
| CN101775386A (en) * | 2010-01-26 | 2010-07-14 | 武汉工业学院 | Method for immobilizing trypsinase by chitosan microspheres |
| CN102662061A (en) * | 2012-04-17 | 2012-09-12 | 北京九强生物技术股份有限公司 | Kit for determination of human alpha-fetoprotein content by latex-enhanced immunoturbidimetry |
| CN102695718A (en) * | 2009-12-28 | 2012-09-26 | 龟甲万株式会社 | Method for extracting staphylococcus aureus antigen, reagent for extracting staphylococcus aureus antigen, and method for testing staphylococcus aureus |
| CN102901810A (en) * | 2012-10-09 | 2013-01-30 | 北京九强生物技术股份有限公司 | Preparation method of latex particles coated with prostate specific antigen-antibody and PSA enhanced turbidimetric immunophelometry kit |
| CN102944679A (en) * | 2012-11-15 | 2013-02-27 | 北京康美天鸿生物科技有限公司 | Kit for performing retinol binding protein detection by using latex turbidimetry |
| CN103073642A (en) * | 2012-08-29 | 2013-05-01 | 深圳伯美生物医药有限公司 | CRP monoclonal antibody nanometer latex microsphere composition and preparation process thereof |
| CN103344763A (en) * | 2013-06-18 | 2013-10-09 | 华东师范大学 | Latex-reinforced AFP detection kit and its preparation method and use |
-
2014
- 2014-02-18 CN CN201410057836.5A patent/CN103852580B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050153370A1 (en) * | 2003-12-30 | 2005-07-14 | 3M Innovative Properties Company | Method of enhancing signal detection of cell-wall components of cells |
| CN1603348A (en) * | 2004-09-14 | 2005-04-06 | 清华大学 | Process for synthesis of monodisperse micron crosslinked polystyrene copolymerization microsphere |
| WO2009039854A2 (en) * | 2007-09-27 | 2009-04-02 | Dako Denmark A/S | Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics |
| CN102695718A (en) * | 2009-12-28 | 2012-09-26 | 龟甲万株式会社 | Method for extracting staphylococcus aureus antigen, reagent for extracting staphylococcus aureus antigen, and method for testing staphylococcus aureus |
| CN101775386A (en) * | 2010-01-26 | 2010-07-14 | 武汉工业学院 | Method for immobilizing trypsinase by chitosan microspheres |
| CN102662061A (en) * | 2012-04-17 | 2012-09-12 | 北京九强生物技术股份有限公司 | Kit for determination of human alpha-fetoprotein content by latex-enhanced immunoturbidimetry |
| CN103073642A (en) * | 2012-08-29 | 2013-05-01 | 深圳伯美生物医药有限公司 | CRP monoclonal antibody nanometer latex microsphere composition and preparation process thereof |
| CN102901810A (en) * | 2012-10-09 | 2013-01-30 | 北京九强生物技术股份有限公司 | Preparation method of latex particles coated with prostate specific antigen-antibody and PSA enhanced turbidimetric immunophelometry kit |
| CN102944679A (en) * | 2012-11-15 | 2013-02-27 | 北京康美天鸿生物科技有限公司 | Kit for performing retinol binding protein detection by using latex turbidimetry |
| CN103344763A (en) * | 2013-06-18 | 2013-10-09 | 华东师范大学 | Latex-reinforced AFP detection kit and its preparation method and use |
Non-Patent Citations (1)
| Title |
|---|
| 王崇义等: "单克隆抗体标志乳胶凝集试验快速检测金黄色葡萄球菌及其耐药性", 《中华医院感染学杂志》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103852580B (en) | 2015-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101216491B (en) | Mycobacterium tuberculosis detection liquid phase chip and method for making same | |
| CN108593641B (en) | Kit and method for quantitatively detecting substance to be detected in whole blood sample | |
| CN104459125A (en) | Method for rapidly detecting gram negative and positive bacteria | |
| CN104007269A (en) | Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof | |
| Leavelle et al. | Staphylococcal clumping on microtiter plates: a rapid, simple method for measuring fibrinogen split products | |
| CN101581726A (en) | New-generation brucellosis antibody competition enzyme-linked immunosorbent adsorption test detection kit | |
| CN105785054A (en) | Chemical-luminescent microfluidic disk for quantitative detection of procalcitonin and using method thereof | |
| CN103529204A (en) | Latex agglutination kit for detecting streptococcus agalactiae antibodies and application of kit | |
| CN102212514A (en) | Blood coagulant for blood collection container | |
| CN101799470A (en) | Brucellosis indirect enzyme-linked immunosorbent assay antibody detection kit | |
| CN101675340A (en) | Method of detecting infection with urogenital mycoplasmas in humans and a kit for diagnosing same | |
| CN103499685B (en) | People's urinary system cause of disease bacterium infects the preparation method of the immunofluorescence diagnostic reagent of clinical diagnosis | |
| CN102507934B (en) | Time-resolved fluoroimmunoassay (TRFIA) of Klebsiella oxytoca | |
| CN103288963B (en) | Monoclonal antibody for kitasamycin residual detection and preparation method and application thereof | |
| CN103852580B (en) | Staphylococcus aureus identification kit and preparation method thereof | |
| CN105158473A (en) | Myeloperoxidase measuring kit | |
| EP0150567B1 (en) | Process for detecting beta-hemolytic streptococcus antigens | |
| CN205910200U (en) | Former quantitative determination's of calcitonin micro -fluidic disc of chemiluminescence | |
| CN203965445U (en) | A kind of dynamic color method limulus kit for endotoxin detection | |
| CN104330562B (en) | Treponema pallidum specific antibody high throughput testing test kit and preparation method thereof | |
| CN103897046A (en) | Receptor assay method and kit for detecting beta-lactam antibiotic residues | |
| CN1318152A (en) | Salmonella antigen formulation and kit for detecting salmonella antibodies | |
| US5093235A (en) | Immuno-dye reagent and assay for detection of endotoxin | |
| CN101004419A (en) | Colloid selenium test paper for detecting pulpy kidney, and type of pathogenesis bacteria | |
| CN103509068A (en) | Amikacin semi-antigen, and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |