CN103833192A - Artificial soil by vermicomposting method of treat sewage sludge using compound microorganism - Google Patents

Artificial soil by vermicomposting method of treat sewage sludge using compound microorganism Download PDF

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Publication number
CN103833192A
CN103833192A CN201310053362.2A CN201310053362A CN103833192A CN 103833192 A CN103833192 A CN 103833192A CN 201310053362 A CN201310053362 A CN 201310053362A CN 103833192 A CN103833192 A CN 103833192A
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CN
China
Prior art keywords
earthworm
sewage sludge
kinds
vermicomposting
processing
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CN201310053362.2A
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Chinese (zh)
Inventor
洪承益
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HIDE Co Ltd
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HIDE Co Ltd
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Priority to KR10-2012-0132125 priority Critical
Priority to KR1020120132125A priority patent/KR20130000366A/en
Application filed by HIDE Co Ltd filed Critical HIDE Co Ltd
Publication of CN103833192A publication Critical patent/CN103833192A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00Treatment of sludge; Devices therefor
    • C02F11/02Biological treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

A manufacturing method of artificial soil is provided to feed worms with sewage sludge treated by using 5 kinds of mixed strain comprising photosynthetic bacteria. 20 to 50 days are required for a decomposition procedure as a preprocessing procedure in a prior method for reating sewage sludge using earthworm. A manufacturing method of artificial soil has advantages that the five kinds of mixed strains including photosynthetic bacteria are mixed with sewage sludge for utilization. Earthworm feeding stability can be improved. Feeding worms with the mixed sewage sludge does not require a separate pre-processing. Besides, stink produced in a processing procedure can be eliminated by mixing the five kinds of the strains including the photosynthetic bacteria in sewage sludge. .

Description

Utilize complex microorganism to carry out dealing with earthworm and the method for manufacture of intraocular soil to sewage sludge
Technical field
The present invention relates to 5 kinds of compound strains that a kind of utilization comprises photosynthetic bacterium sewage sludge is carried out after primary treatment, feed to earthworm, carry out dealing with earthworm and the method for manufacture of intraocular soil.
Background technology
In food chain, earthworm and microorganism are the decomposing organisms being positioned at bottom, are the Omnivores take movement, protozoon, nematode, dirt mite, Collembola, bacterium, yeast and the mushroom etc. of the residue of each kind of plant, phytophagous animal as food.Become the body weight of earthworm to be about 0.4g, eat the food of 0.8 ~ 1.5 times of own body weight every day, and the food absorbing is pulverized in the bowel movement of health link by 120 ~ 130 left and right, digest food in mobile process simultaneously, earthworm can only digest and assimilate the food of the scheduled time of becoming thoroughly decomposed, this be because the zymigen having due to earthworm itself is few its function a little less than, therefore can only with intestines in microorganisms symbiosis time just can carry out digestion process.That is, earthworm does not secrete the gland of strong acid, and for protein and carbohydrate are transformed to energy or nutrition source, strong acid is necessary key element, therefore, and necessary and microorganisms symbiosis.And, in earthworm intestines, though readily digested and kill most yeast and other poor microorganisms of defense function, local bacterium and actinomycetes survival and be replicated thousands of times during in earthworm intestines, and help the food decomposing and digestion is absorbed.The organic sludge of supplying as the food of earthworm is the organic substance that roughly contains microorganism, therefore, by the effect of the microorganism in earthworm intestines, after the time, sharply change, this change procedure is called to the processing or the Vermicomposting (Vermicomposition) that utilize earthworm.But, utilize earthworm to become thoroughly decomposed while processing to organic sludge, should meet the physiological status of earthworm as the physics-chem characteristic of the organic sludge of the food of earthworm, and must not contain the virose material of earthworm tool.But, feed in organic sludge to earthworm, the strictest to the requirement of sewage sludge, in the time feeding to earthworm, first mud carry out anaerobic fermentation, and could supply earthworm to absorb through after 20 ~ 50 days become thoroughly decomposed.This is the result that obtained after deliberation both at home and abroad.
Analysis can be used as the result of the feeding condition of the sewage sludge of feed, find for mud being dewatered in most of sewage sludge, and through have-more than 100 value of the redox potential of concentrated mud, this is due to for mud is dewatered, use inorganic or polymer coagulant and make the value of pH value higher than the suitable factor of growth of the earthworm of recording in various documents, and alkaline numerical value major part is also high and be not suitable as the feed of earthworm, therefore, need to carry out pre-treatment.
Therefore, the existing dealing with earthworm device that utilizes earthworm, the operation of becoming thoroughly decomposed that needs 20 ~ 50 days as pretreatment procedure.As mentioned above, this is because because the digestion organs of earthworm are very single, and lacks the lytic enzyme of the strong acid for once decomposing, and after therefore first decomposing by microorganism etc. in earthworm intestines, is ingested and digests.But conventionally, sewage sludge is difficult to the feed as earthworm because organic components is low, but has the microorganism of many types in water treatment operation due to breeding, be therefore easy to produce by microorganism the operation of becoming thoroughly decomposed.
But, utilize the treatment technology of the organic sludge of earthworm, if can guarantee treatment effect and stability, the advantage of decomposing compared with biology and guarantee other modes such as usability of the best by product of what is called of earthworm ight soil soil, is outstanding treatment technology, but need to increases aerobic composting device or the operation of becoming thoroughly decomposed as pretreatment procedure, therefore exist setup fee high, the problem that effect is undesirable, and, be difficult to significantly improve existing method and develop effective treatment technology.
Summary of the invention
Aspect environment, Korea S's original function of earthworm that begins one's study nineteen ninety, and advance feature research topic and G-7 project cause by the supervisor of Science and Technology Branch of state-run environmental science institute of Korea S, to establish scientific research basis, and set up framework roughly.Based on this, the treatment process that the organic sludge that utilizes earthworm has been admitted in environment in May, 1992 place notices No. 1992-34 by Department of the Environment, and formally assert the environmental purification ability of earthworm, and, revise waste management method in July, 1997, based on existing viewpoint, following viewpoint has further been carried out to legalization, when being about to utilize organic sludge as fertilizer sources, compost or the feed that earthworm processes and using, can carry out manipulation of regeneration.
In April, 2004, it is domestic animal that department of agriculture and forestry notices earthworm, in February, 2007, is included in farmland method using earthworm canopy as agricultural facility, and has significantly improved the institutional restriction for dealing with earthworm, also actively encourages the raising of earthworm.But in the time utilizing dealing with earthworm organic sludge, the pass of earthworm and feed is most important factor.That is, be the organic substance that contains microorganism as the organic sludge of the feed of earthworm, therefore, after certain hour, occurred to rot or fermentation by microorganism, this phenomenon is called to become thoroughly decomposed (Decomposition).
Therefore, in order to utilize earthworm to process organic sludge as above, the physics-chem characteristic of mud itself should meet the physiological status of earthworm, and does not contain the virose material of earthworm tool in the naturally rotten process of mud., only, in the time that the physical and chemical condition of mud meets the raising condition of earthworm, could utilize earthworm to process.Therefore, sewage sludge is fed while processing to earthworm, because sewage sludge carries out anaerobic fermentation step, therefore need could supply earthworm to absorb through the rotten process of 20 ~ 50 days.
Following table 1 represents the suitable factor of the suitable feeding condition of Vermicomposting.
[table 1]
The present invention relates to 5 kinds of compound strains that a kind of utilization comprises photosynthetic bacterium carries out after primary treatment sewage sludge, by directly feeding and carry out composting method and the method for manufacture of intraocular soil to earthworm, the effect and the feature that the greening of earthworm be describeds or colonize in the microorganism of preponderating in the bacterial strain of the microorganism in earthworm intestines kind are as follows.
First, Rodobacter capsulata(photosynthetic bacterium), in the time of general light compositing, bacterium itself does not have photosynthetic pigments, therefore, though can not oneself carry out photosynthesis, but owing to thering is bacteriochlorophyll, therefore as material use carbonic acid gas and hydrogen compound (H 2s etc.) carry out photosynthesis and manufacture sugar.Most higher plant is reducing substances, uses hydrogen sulfide (H 2s) replace water (H 2o) gas, producing in this process neither oxygen (O 2) but sulphur (S).Described photosynthetic bacterium (Rodobacter capsulata), in sewage sludge of the present invention is processed, under aerobic dark condition (Aerobic dark), utilizes N 2, NH 3electron acceptor(EA) breed, and convert the unsettled ammonia-state nitrogen of main component in organism to stable nitric nitrogen, thereby increase the feeding efficiency of earthworm, under anaerobic illumination condition (Anaerobic light), utilize H 2the electron acceptor(EA) of S is bred, and have, the anaerobism that prevents sewage sludge is become thoroughly decomposed and the effect of pH buffering (Buffering).
Bacillus megaterium (Bacillus megaterium) is the microorganism that protein capacity of decomposition is outstanding,
In earthworm intestines, with sociales symbiosis, and decompose the protein high molecular that absorbs.The ability of the polymer coagulants such as the acrylamide that decomposition is used as the polymer coagulant of sewage sludge is also very outstanding, the cohesion structure of the dehydration of destruction obstruction sewage sludge and dry mud, improve the degrading activity of microorganism, and then improve processing efficiency.
Genus bacillus (Geobacillus stearo ther mophilus) is high warm nature microorganism, and its fermentative processing speed is very fast, utilizes this characteristic mainly to use the thermophilic fermentation at swill.In the present invention, microorganism as above is put in sewage sludge and carries out, after fermentative processing, being injected in earthworm intestines and inoculating, thereby carry out fermentative processing in earthworm intestines.
Genus bacillus (Geobacillus stearothermophilus) is heat-loving microbe, to be present in the original air in ancient times and the ancient microorganism in sea, even if also can breed in extreme environment and activity, and under the alkaline condition of polymer coagulant that utilizes sewage sludge, also there is the ability of outstanding decomposing organic matter, thereby can colonize in earthworm intestines as the green micro organism of earthworm.
Rhodococcus (Rhodococcus sp.) is the outstanding bacterial strain of ability that decomposes the phenol compounds such as toluene, described toluene is the hard-decomposed compound that is contained in sewage sludge, described rhodococcus relaxes the hazardous property of the environmental hormone that is contained in sewage sludge, and effectively removes stench.
5 kinds of compound strains that comprise the photosynthetic bacterium that described organic capacity of decomposition is outstanding are cultivated respectively to x10 8, and each microorganism is mixed to same amount and the microbial culture medium manufactured, with 10 liters: the ratio of 1 ton is mixed into sewage sludge, and sewage sludge is not carried out to other pretreatment procedure and just feed to earthworm safely, and use as the feed of earthworm.
The present invention relates to 5 kinds of compound strains that a kind of utilization comprises photosynthetic bacterium carries out after primary treatment by feeding the method for carrying out manufacture of intraocular soil to the composting method of earthworm sewage sludge, more specifically, 5 kinds of compound strains of the photosynthetic bacterium outstanding ability that comprises decomposing organic matter (Rhodobacter sphaeroides, Rhodobacter capsulata, Bacillus megaterium, Geobacillus stearothermophilus, Rhodococcus sp.) are cultivated to x10 8, and the microbial culture medium that each microorganism mixing same amount is manufactured, with 10 liters: the ratio of 1 ton is mixed into sewage sludge, and sewage sludge is not carried out to other pretreatment procedure and just can feed to earthworm safely.Thus, without during to carry out the pre-treatment of 20 ~ 50 days in order carrying out with dealing with earthworm sewage sludge and to become thoroughly decomposed, therefore when significantly reducing whole treatment time and processing costs and space, can stably carry out dealing with earthworm, and, the stench because rotting to produce occurring in dealing with earthworm process also can be reduced to the level that people is difficult to smell effectively.
Accompanying drawing explanation
Fig. 1 is the photo of the artificial soil that completes according to the present invention.
Fig. 2 illustrates the chart changing by the pH of compound strain of the present invention sewage sludge after treatment.
Fig. 3 illustrates the chart changing by the ORP of compound strain of the present invention sewage sludge after treatment.
Fig. 4 illustrates by the check plot of compound strain of the present invention sewage sludge after treatment and the NH of trial plot 3-N and NO 3the chart of the change in concentration of-N.
Embodiment
The present invention relates to 5 kinds of compound strains that a kind of utilization comprises photosynthetic bacterium carries out after primary treatment sewage sludge, carry out composting method and the method for manufacture of intraocular soil by feeding to earthworm, more specifically, by the earthworm greening of the photosynthetic bacterium that comprises the outstanding and greening environment of organic capacity of decomposition or colonize in 5 kinds of compound strain (Rhodobacter sphaeroides in earthworm intestines, Rhodobacter capsulata, Bacillus megaterium, Geobacillus stearothermophilus, Rhodococcus sp.) difference isolation identification, and cultivate x10 8, then each microorganism is mixed to same amount and the microbial culture medium manufactured with 10 liters: the ratio of 1 ton is inoculated into sewage sludge mixes, and just causes and can feed safely the condition to earthworm thus without sewage sludge being carried out to other pretreatment procedure.
[embodiment 1]
Relatively according to embodiments of the invention and other embodiment, the variation of sewage sludge being fed to during to earthworm (in figure, with ■ represent) without any processing, sewage sludge is carried out to () while input after the aerobic composting pretreatment procedure of 50 days, 5 kinds of compound strains that comprise photosynthetic bacterium of the present invention being inoculated into the pH value of 35 days of (▲) while dropping into after sewage sludge refers to Fig. 2.
; 5.8 ~ 7.5 for stably keeping the scope of the pH value of the picked-up condition of earthworm; in the time feeding sewage sludge separately to earthworm; after 14 days, start pH value and be not suitable for the growth conditions of earthworm; as the present invention; when 5 kinds of compound strains that comprise photosynthetic bacterium are inoculated into sewage sludge, the variation of its pH value is similar to the aerobic composting condition during 50 days, maybe can keep more stable condition.
[embodiment 2]
Refer to Fig. 3 according to the variation of the redox potential during 35 days of the present invention.
, for the scope of redox potential of the stable feeding condition that keeps earthworm is more than >0mV, in the time individually sewage sludge being dropped into without any processing, drop into and start sharply to occur becoming thoroughly decomposed after 5 days, when drop into through the aerobic composting condition of 50 days sewage sludge time, drop into and start sharply to occur becoming thoroughly decomposed after 10 days, be not suitable for the growth conditions of earthworm since the 14th day, but when the 5 kinds of compound strains of the present invention that comprise photosynthetic bacterium are inoculated into sewage sludge, at least till putting into 25 days, keep stable feeding condition.
[embodiment 3]
In the present invention, check plot is to become thoroughly decomposed pre-treatment after 50 days, the NH producing sewage sludge being carried out to aerobic in mud 3-N (◆) and NO 3the change in concentration of-N (), trial plot be by comprise photosynthetic bacterium 5 in compound strain be inoculated into after sewage sludge, observe each change in concentration (referring to Fig. 4) of 8 days.
Known, in the sewage sludge of the pretreatment procedure that becomes thoroughly decomposed through aerobic, the NH producing in mud 3-N and NO 3the amount of the change in concentration of-N to ammonia-state nitrogen and the amount of nitric nitrogen do not have large variation, and when 5 kinds of compound strains that comprise photosynthetic bacterium are inoculated into sewage sludge, the NH in mud 3-N and NO 3the change in concentration of-N increases respectively 2 ~ 5 times than the ratio of ammonia-state nitrogen.
Thus, by the activity of the compound strain through Combined Processing, sewage sludge is changed to the form of easily being digested and assimilated by earthworm, thereby improve the uptake ratio of earthworm, and, to can not be separately feeding sewage sludge to earthworm without carrying out aerobic pretreatment procedure, also can obtain equal above treatment effect.

Claims (3)

1. a method for manufacture of intraocular soil, is characterized in that, 5 kinds of compound strains that comprise photosynthetic bacterium is blended in to sewage sludge and processes rear feeding to earthworm, to carry out Vermicomposting processing.
2. the method for manufacture of intraocular soil according to claim 1, it is characterized in that, 5 kinds of compound strains comprise Rhodobacter sphaeroides, Rhodobacter capsulata, Bacillus megaterium, Geobacillus stearothermophilus, Rhodococcus sp., these microorganisms and sewage sludge are carried out to combination treatment, then, sewage sludge is fed and carried out Vermicomposting processing to earthworm without other pretreatment procedure.
3. the method for manufacture of intraocular soil according to claim 1, is characterized in that, 5 kinds of compound strains are cultivated respectively to x10 8, and the microbial culture medium that each microorganism mixing same amount is manufactured is with 10 liters: the ratio of 1 ton is inoculated into sewage sludge, then, sewage sludge is fed and is carried out Vermicomposting processing to earthworm without other pretreatment procedure.
CN201310053362.2A 2012-11-21 2013-02-19 Artificial soil by vermicomposting method of treat sewage sludge using compound microorganism Pending CN103833192A (en)

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KR1020120132125A KR20130000366A (en) 2012-11-21 2012-11-21 Artificial soil by vermicomposting method of treat sewage sludge using compound microorganism

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN107032838A (en) * 2017-05-16 2017-08-11 华南农业大学 A kind of municipal sludge base Nutrition Soil and preparation method thereof

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CN104941993B (en) * 2015-07-22 2017-09-12 句容市雾起山家庭农场 A kind of method for comprehensive repairing in serious pollution soil
US20190000124A1 (en) * 2017-07-03 2019-01-03 Oakbio, Inc. Novel Microbial Biomass Based Feed Products
CN107915377B (en) * 2017-12-12 2020-11-10 陕西祥宇环保科技有限公司 Urban domestic sewage treatment method
CN108690819B (en) * 2018-05-30 2020-03-17 叶荫人 Geobacillus stearothermophilus strain and application method thereof
CN110404958A (en) * 2019-06-19 2019-11-05 上海市环境科学研究院 A method of utilizing Trichoderma and earthworm interaction remediating heavy metal and organic pollutant combined contamination soil

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JP2000063832A (en) * 1998-08-21 2000-02-29 Kimisato Seimei Kogaku Kenkyusho:Kk Soil improver
KR20010009772A (en) * 1999-07-13 2001-02-05 이원서 A process of organic waste articles used vermiculture
JP2001062494A (en) * 1999-08-26 2001-03-13 Takaaki Arai Treatment of waste paper sludge
KR20080013839A (en) * 2007-12-27 2008-02-13 영농조합법인이엔비 Vermicomposting method treat sewage sludge by compound microorganism using without pretreatment process

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000063832A (en) * 1998-08-21 2000-02-29 Kimisato Seimei Kogaku Kenkyusho:Kk Soil improver
KR20010009772A (en) * 1999-07-13 2001-02-05 이원서 A process of organic waste articles used vermiculture
JP2001062494A (en) * 1999-08-26 2001-03-13 Takaaki Arai Treatment of waste paper sludge
KR20080013839A (en) * 2007-12-27 2008-02-13 영농조합법인이엔비 Vermicomposting method treat sewage sludge by compound microorganism using without pretreatment process

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107032838A (en) * 2017-05-16 2017-08-11 华南农业大学 A kind of municipal sludge base Nutrition Soil and preparation method thereof

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