CN103773814B - A kind of sugar grass prepares the method for alcohol fuel - Google Patents
A kind of sugar grass prepares the method for alcohol fuel Download PDFInfo
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Abstract
The invention discloses a kind of method that sugar grass prepares alcohol fuel.The method includes: the fresh bar of sugar grass is squeezed by (1), it is achieved juice, slag separate；(2) the sugar grass syrup obtained concentrates, and prepares into molasses；(3) sweet sorghum slag obtained is pulled out after utilizing dilute acid soln immersion and is dried；(4) sweet sorghum slag that step (3) obtains carries out dilute acid pretreatment or dilute acid steam explosion pretreatment；(5) pretreated sweet sorghum slag add in alkali and after, add cellulase degradation, the solid content of enzymatic hydrolysis system is 5wt%-35wt%, obtains sweet sorghum slag enzymolysis solution after enzymolysis；(6) molasses are joined producing fuel ethyl alcohol by ferment in sweet sorghum slag enzymolysis solution in the ratio of 0-30wt%；(7) ethanol that fermentation is produced is easily separated, it is thus achieved that process of alcohol products.The method can realize the long-term preservation of sweet sorghum slag, it is achieved juice, the effective of slag utilize, it is ensured that the whole year production of alcohol fuel, has advantage efficient, energy-saving and environmental protection.
Description
Technical field
The present invention relates to a kind of method that sugar grass prepares alcohol fuel, be specifically related to a kind of method utilizing the sugar of cellulose hydrolysis in sweet sorghum stalk and syrup to prepare alcohol fuel.
Background technology
Alcohol fuel is a kind of Novel clean-fuel, is the important directions of regenerative resource exploitation.Alcohol fuel can be produced by biofermentation mode with non-grain crops such as the cereal crops such as Semen Maydis, Semen Tritici aestivi and Caulis Sacchari sinensis, Maninot esculenta crantz., Radix Ipomoeae, Sorghum vulgare Pers., Radix Betaes, it is also possible to utilizes Plant fiber through pretreatment, mineral acid or cellulase hydrolysis, produce again through biofermentation mode.Being blended by a certain proportion of alcohol fuel and namely reconcile into ethanol petrol at regular gasoline, in China and U.S.'s vehicle-use alcohol gasoline, ethanol content is 10v%, ethanol content average out to 22v% in Brazil's vehicle-use alcohol gasoline.Ethanol petrol can be effectively improved performance and the quality of oil product, reduces the discharge of the major pollutants such as carbon monoxide, Hydrocarbon.
China announces Car-Use Ethanol Gasoline Promotion in April calendar year 2001, have approved Jilin Fuel Ethanol Co., Ltd, Henan Tian Guan alcohol fuel company, 4 alcohol fuel pilot business of Fengyuan Biological Chemistry Co., Ltd., Anhui Prov. and Heilongjiang China Resources Alcohol Co., Ltd (existing COFCO Biochemical Energy (Zhaodong) Co., Ltd.), promoted in 9 provinces and cities in the whole nation.In the end of the year 2005, China becomes the big fuel ethanol production state in third place in the world.2010, China's alcohol fuel yield reached 1,800,000 tons, but many with cereal crops such as Semen Maydis, Semen Tritici aestivi and rice for raw material (except Beihai Fisheries Base Guangxi Province 200,000 tons/a Cassava-based ethanol).Being restricted by factors such as grain raw material supplies, country's Biological Energy Industry policy turns to the development giving priority to non-grain fuel ethanol industrial, 2007, and National Development and Reform Committee's approval has been given an written reply Guangxi China Oil and Food Import and Export Corporation and produced 200000 tons of Maninot esculenta crantz. alcohol fuel demonstration projects per year.According to " Eleventh Five-Year Plan outline ", to the year two thousand twenty, China's alcohol fuel year utilization is up to 10,000,000 tons, and raw materials for production will based on non-grain crop.
China has a large population and a few land, cultivated land resource is in short supply, grain supply and demand is in tight balance, the grain security of country will be threatened for raw material production alcohol fuel with Semen Maydis, Semen Tritici aestivi, affect grain regular supply, and cause the chain reactions such as agricultural product price rise, so China strictly control with grain be raw material alcohol fuel newly-built and expand can project.In JIUYUE, 2007, National Development and Reform Committee issues " instruction about promoting that corn deep processing industry develops in a healthy way ", point out that the Eleventh Five-Year Plan period China no longer checks and approves newly-built corn deep processing project in principle, require that various places stop corn deep processing project of putting on record immediately, and to building, projects awaiting construction make an overall screening, to putting on record, the project not yet built is halted comprehensively, and the non-cereal crops of encouragement development are development of raw materials alcohol fuel energetically.Although visible alcohol fuel has good use and promotional value in China, but its development trend should, based on China's national situation, be accomplished not strive grain with people, does not strive ground with grain, walks with non-grain crop, such as the production line being raw material with sugar grass.
Sugar grass is a mutation of Sorghum vulgare Pers., originate from Africa, this crop is waterlogging, drought-enduring, Salt And Alkali Tolerance, adaptability is extremely strong, it is referred to as " camel in crop ", be in recent years one of people's energy crop comparing concern (Yan Feng. China's Sweet Sorghum Industry development prospect is probed into. China sugar material, 2010,02:75-76).Sugar grass is carbon 4 plant, has high photosynthetic rate, and biological yield is high, it not only per hectare results 1.5～6.0t Sorghum vulgare Pers. seed, it is often more important that per hectare can produce the 45.0～67.5t stem stalk rich in sugar (sugary 17wt%～21wt%).It is that squeezing season is too short that current sugar grass prepares the subject matter of alcohol fuel existence so that its processing period is only 2～3 months, and effective production time is short, causes investment excessive.Therefore the effectively prolongation problems demand stored with processing period of raw material solves, and to process substantial amounts of residue after processing ethanol, and residue treatment puts into and fermentation technology higher with difficulty and equipment imperfection, causes work efficiency low, and cost is high, and up-front investment is many.
CN200710304647.3 provides a kind of method of preparing ethyl alcohol from sweet sorghum, sugar grass through storing is carried out crushing, squeezing by the method, the directly sugar grass syrup fermentative production of ethanol to squeezing gained, wherein the storage of sugar grass adopts fresh bar to lie low stacked stacking the method spraying microbicide solution on fresh bar.CN201010241725.1 provides a kind of method that fermenting sweet sorghum stem stalk concentrated juice produces alcohol fuel, the syrup of concentration need not additionally be added preservative and both can be stored in basin, but needs to be diluted to concentrated molasses required syrup concentration before using.CN201010217263.X provides a kind of method utilizing Fuel Ethanol Production from Sweet Sorghum Stalk, thaws, carry out pre fermentation and prepare into alcohol fuel with liquid fermentation after then carrying out squeeze juice extracting, concentration after sweet sorghum stalk carries out stored frozen.CN200810072988.7 provides a kind of method improving sweet sorghum comprehensive utilization value, the stem stalk juice of sugar grass, slag are separated, and crushing juice rate controls in 45wt%-65% storage of fermenting, slag is carried out ensiling or skin slag is dried moisture≤20% by the separation of skin marrow, the sugar that to be retained in slag certain is used as the nutritional labeling of feedstuff and microbial, it is ensured that the value of slag.CN200810057713.6 provides a kind of method utilizing sweet sorghum stalk to produce ethanol, this method is by after the stalk juice of squeezing, sweet sorghum slag pretreatment serosity and the mixing of enzymolysis sugar liquid, mixed liquor is carried out steaming and decocting concentration, obtain the mixed sugar liquid of sugar concentration 16wt%-24wt%, the sugared concentration required to ensure fermentation.
Although with sugar grass for raw material production alcohol fuel achieved with greater advance, the storage of sugar grass can adopt fresh sweet sorghum stalk spraying antibacterial or/and preservative, fresh stem stalk chilled storage, concentration sweet sorghum juice or instant fermentation store the methods such as karusen, to alleviate sugar loss in the fresh bar of sugar grass, it is achieved whole year production.But, adding of germicide is or/and subsequent fermentation is had inhibitory action by preservative, and to environmentally undesirable；Outdoor refrigerated storage sugar loss is big, is subject to Changes in weather impact, it is easy to go mouldy, and the floor space storing fresh sweet sorghum stalk is big；Instant fermentation stores karusen poor operability；It is high that concentration improves sugar liquid osmotic pressure energy consumption, also needs dilution to cause energy dissipation during subsequent fermentation, and the process of sweet sorghum slag and be used without good solution, namely cause the wasting of resources, bring again environmental problem.Therefore, want that the large-scale industrial production realizing sugar grass comprehensive utilization and whole year production alcohol fuel also has certain distance in engineering amplifying technique, product economy.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of method that sugar grass prepares alcohol fuel.The method can realize the long-term preservation of sweet sorghum slag, it is achieved juice, the effective of slag utilize, it is ensured that the whole year production of alcohol fuel, has advantage efficient, energy-saving and environmental protection.
Sugar grass of the present invention is prepared the method for alcohol fuel and comprises the following steps:
(1) the fresh bar of sugar grass is squeezed, it is achieved juice, slag separate；
(2) the sugar grass syrup obtained concentrates, and prepares into molasses；
(3) sweet sorghum slag obtained is pulled out after utilizing dilute acid soln immersion and is dried；
(4) sweet sorghum slag that step (3) obtains carries out dilute acid pretreatment or dilute acid steam explosion pretreatment；
(5) pretreated sweet sorghum slag add in alkali and after, add cellulase degradation, the solid content of enzymatic hydrolysis system is 5wt%-35wt%, obtains sweet sorghum slag enzymolysis solution after enzymolysis；
(6) molasses are joined producing fuel ethyl alcohol by ferment in sweet sorghum slag enzymolysis solution in the ratio of 0-30wt%；
(7) ethanol that fermentation is produced is easily separated, it is thus achieved that process of alcohol products.
In the present invention, sugar grass can defoliation before squeezing the juice, it is possible to defoliation does not directly squeeze, squeeze pressure is 30-60MPa, and crushing juice rate is 60wt%-85wt%, and in sweet sorghum juice, sugar content is 10wt%-20wt%, in sweet sorghum slag, content of cellulose is 30wt%-40wt%, and hemicellulose level is 20wt%-30wt%.
In the present invention, sugar grass syrup need to concentrate the molasses that saccharogenesis concentration is 40wt%-60wt%, and to ensure that sugar juice osmotic pressure can suppress growth of microorganism, the method for concentration can be that all can to the equipment of sugar juice concentration, including evaporation, multiple-effect evaporation or membrane filtration etc., it is preferable that multiple-effect evaporation.The molasses of concentration preparation need not additionally add preservative and just can preserve for a long time, and sugar therein will not lose, it is to avoid affect thalline subsequent fermentation because of preservative and produce ethanol.The molasses of concentration may be used for alcohol fermentation bacterium seed culture or activation, or can also be directly used as the raw material production alcohol fuel of alcohol fermentation, but needs to be diluted to sugar concentration 15wt%-25wt%, and this concentration range does not affect alcohol fermentation growth.
In the present invention, the sweet sorghum slag that water content is 10wt%-50wt% is placed in the dilute acid soln of 2wt%-20wt% and soaks 0.5-24h, the hydrion in diluted acid is made fully to penetrate in the moisture of sweet sorghum slag, then the sweet sorghum slag of pickling is pulled out from diluted acid, drip-dry moisture, natural air drying is 10wt%-30wt% to water content, stacks and preserves.The diluted acid used can be all acid compounds, preferably employing sulphuric acid, the purpose of pickling is to prevent fresh sweet sorghum slag from rotting, it is achieved long-term preservation, ready access upon use, wherein free sugar will not lose, it is to avoid affects thalline subsequent fermentation because of preservative and produces ethanol；Acid soaking step can as the pre-treatment step of next step dilute acid steam explosion pretreatment simultaneously.Sweet sorghum slag can substitute with all other lignocellulosic materials, it is possible to is that all contain cellulosic biological material, including wood, corn straw and Caulis et Folium Oryzae etc..
In the present invention, the condition of dilute acid pretreatment is acid concentration 0.1wt%-5.0wt%, solid content 5wt%-35wt%, temperature 120-200 DEG C, processes time 0.5-5.0h.Steam explosion pretreatment condition is temperature 140-200 DEG C, digestion time 3-10min, pressure 0.5-0.9MPa.Pretreatment is by the hemicellulose in the method hydrolysis sweet sorghum slag of physics and chemistry and partial lignin, makes the cellulose wrapped up by hemicellulose and lignin in sweet sorghum slag come out, and becomes relative loose, it is simple to the absorption of cellulase and enzymolysis.The equipment that steam explosion pretreatment adopts can be continuous steam demolition set or batch steam blasting device of all existing molding, it would however also be possible to employ other similar devices developed or developing in the future.Moisture 5wt%-40wt%, xylose 5wt%-20wt%, glucose 1wt%-10wt%, fructose 3wt%-5wt%, cellulose 25wt%-45wt%, hemicellulose 0.5wt%-21wt% in the sweet sorghum slag butt obtained after pretreatment.
In the present invention, pretreated sweet sorghum slag adds alkali compounds, such as NaOH, Ca (OH)2Or ammonia etc., neutralizing pH is 4.5-5.5.Sweet sorghum slag after neutralization prepares into enzymatic hydrolysis system with cellulase and water, and sweet sorghum slag can in initial disposable addition, it is possible to batch feeding adds, and sweet sorghum slag batch feeding adds the viscosity that can reduce initial system, is conducive to reaction system to be mixed evenly.The addition of cellulase is 5-50IU/g cellulose, and the solid content of enzymatic hydrolysis system is 5wt%-35wt%, and enzymatic hydrolysis condition is: pH is 4.5-5.5, and temperature is 45-55 DEG C, and stir speed (S.S.) is 5-300rpm, and enzymolysis time is 24-120h.Enzyme digestion reaction can carry out in vertical mixing tank or bedroom stirred reactor, it is possible to use all exclusively for lignocellulose raw material enzyme digestion reaction design similar batch or continuous enzymolysis reaction unit react.
Ethanol mass concentration and substrate useful load that sweat is final are directly relevant, and therefore, in order to obtain high ethanol mass concentration, solid content should be high as much as possible.But, the solid content in substrate is too high, then mash viscosity can be caused higher, it is necessary to stirring energy consumption excessive.The fermentation of sweet sorghum slag enzymolysis solution is utilized to generate in the process of ethanol, enzymolysis process solid content is more high, and it is also more high that final enzymolysis produces concentration of glucose, but the mass transfer that solid content increase can cause enzymolysis is uneven, cause enzymolysis glucose yield to reduce, affect cellulosic utilization in raw material.When in enzymatic hydrolysis system of the present invention, solid content is more than 35wt%, have a strong impact on the mass transfer of enzyme digestion reaction, if directly utilizing the fermentation of sweet sorghum slag enzymolysis solution to generate ethanol, owing to the solid content of enzymatic hydrolysis system limits, sugar concentration (glucose and xylose) maximum only 14wt% in enzymolysis solution, theoretical ethanol production only 7v%, subsequent ethanol separating energy consumption can be higher.The solid content of preferred enzyme enzymatic hydrolysis system of the present invention is 5wt%-20wt%, although the glucose that enzymolysis produces can reduce, but enzyme digestion reaction system mass transfer is good, and glucose yield can increase.
In the present invention, sweet sorghum slag enzymolysis solution is the solid-liquid mixed liquor after enzymolysis, or is the enzymolysis sugar juice obtained after enzymolysis mixed liquor solid-liquid separation, it is preferable that is directly added into molasses without solid-liquid separation after enzymolysis, carries out band slag fermentation.Have the following advantages: (1) sweet sorghum slag after enzymolysis has portion of cellulose and hemicellulose to remain in insolubility solid possibly, stay and subsequent fermentation system can continue hydrolysis during the fermentation, it is achieved simultaneous saccharification and fermentation；(2) soluble solid content discarded in solid-liquid separation process can take away the solubility fermentable sugars that Partial digestion goes out, cause part sugar loss, without solid-liquid separation, carry out the fermentation of band slag and can reduce this partial loss.
After enzymolysis, the concentration of glucose in enzymolysis solution is 2wt%-15wt%, and xylose concentration is 1wt%-6wt%.The sugared concentration of fermentation liquid is preferably 15wt%-25wt%, it is ensured that the osmotic pressure that alcohol fermentation bacterium can grow, and determines the additional proportion of molasses according to the sugared concentration of fermentation liquid and enzymolysis solution, and the additional proportion of molasses is preferably 10wt%-30wt%.
In the present invention, alcohol fermentation includes alcohol fermentation bacterium seed culture or activation, switching and the fermentation of alcohol fermentation bacterium generate ethanol, wherein alcohol fermentation strain includes all to glucose, sucrose, fructose and wood-sugar fermentation generate the bacterial strain of ethanol, preferably saccharomyces cerevisiae or adopt commercially available active yeast, and/or zymomonas mobilis, detailed process is as follows: (1) alcohol fermentation bacterium seed culture or activation: alcohol fermentation bacterium seed culture includes the molasses to be diluted to sugar content 1wt%-5wt% as culture matrix, from strain inclined plane, scrape 1-2 ring bacterium mud be inoculated into Shake flask medium, then amplify step by step, amplification is every grade 10-100 times, until being inoculated in fermentation medium；Alcohol fermentation seed activation includes molasses being diluted to sugar content 1wt%-5wt% as culture matrix, weighs the active microorganism dry powder of q.s, it is preferable that dry powder consumption is the 0.05wt%-0.50wt% of fermentation medium；(2) switching of alcohol fermentation bacterium: be inoculated into fermentation medium from seed culture fluid or seed activation liquid by fermented bacterium, inoculum concentration is 1wt%-10wt%；(3) fermentation generates ethanol: is inoculated into by alcohol fermentation strain in fermentation medium and cultivates, and metabolism produces ethanol.Fermentation medium also includes the nitrogenous source needed for growth of microorganism and inorganic salt, and 121 DEG C of sterilizing 0.5-1h are standby.Nitrogenous source can be the available nitrogen-containing compounds of all microorganisms, such as carbamide, ammonium sulfate, yeast extract etc., it is preferable that carbamide.Inorganic salt can be the inorganic salts compound needed for all growth of microorganism, such as phosphate, sulfate, magnesium ion, calcium ion and iron ion etc..
In the present invention, in fermentation liquid, the separation of ethanol includes all conventional ethanol separation methods, such as rectification method.
Sugar grass of the present invention is prepared the method for alcohol fuel and has the advantage that (1) carries out different disposal after being separated by fresh squeezing sorgo juice slag respectively, after utilizing dilute acid soln to soak sweet sorghum slag, pulls out and dries, it is achieved the long-term preservation of sweet sorghum slag.Diluted acid can be used as preservative and preserves the part sucrose, glucose and the fructose composition that remain in sweet sorghum slag in juice-extracting process, it is prevented that raw material goes bad, it is achieved the whole year production of alcohol fuel；It is also used as the catalyst of sweet sorghum slag dilute acid steam explosion pretreatment, improves the effect of follow-up pretreatment；(2) the fresh juice of sugar grass is in order to preserve wherein sugar the whole year, and it is a kind of effective method that molasses are prepared in concentration, but needs dilution before using, and causes energy dissipation.Utilize in sweet sorghum slag sugar as alcohol fermentation nutritional labeling, it is achieved that effective utilization of sweet sorghum slag, improve raw material utilization efficiency so that the value of sugar grass maximizes, but due to the restriction of solid content, the problem that there is sugared concentration deficiency；The present invention adds appropriate molasses mixed fermentation in sweet sorghum slag enzymolysis solution, both can ensure that the sugared concentration of fermentation system, it is not required that molasses are diluted, cause energy dissipation, overcome the solid content restriction of enzymolysis and sweat simultaneously, reduce subsequent ethanol separating energy consumption.The present invention effectively utilizes cellulose and hemicellulose sugar fermentation in the syrup in the fresh bar of sugar grass, sweet sorghum slag to generate alcohol fuel, improves final ethanol production.
Detailed description of the invention
The sugar grass further illustrating the present invention by the examples below prepares the method for alcohol fuel.In the present invention, wt% is mass fraction, and v% is volume fraction.
The fresh bar of embodiment 1 sugar grass is squeezed the juice
Taking the fresh bar of sugar grass of 500kg fresh harvesting defoliation and fringe, squeeze with commercially available small-sized presser, squeeze pressure is 40MPa, it is thus achieved that sweet sorghum juice 365kg, sweet sorghum slag 135kg.In sweet sorghum juice, sucrose concentration is 11.5wt% after measured, and concentration of glucose is 3.05wt%, and fructose concentration is 2.55wt%；In sweet sorghum slag, water content is 50wt%, and content of cellulose is 35.6wt%, and hemicellulose level is 24.3wt%.
Embodiment 2 prepares sugar grass molasses
Squeeze the juice in Example 1 the sugar grass syrup 300kg obtained, and utilizes Rotary Evaporators to concentrate, and finally gives 100kg sugar grass molasses, and total sugar concentration is 51.3wt%, and sucrose concentration is 34.5wt%, and concentration of glucose is 9.15wt%, and fructose concentration is 7.65wt%.
Embodiment 3 sweet sorghum slag pickling preservation
Example 1 squeezes the sweet sorghum slag 100kg of acquisition, is divided into 10 parts, every part of 10kg.Take a 15L glass container again, add the dilute sulfuric acid of 10L5wt%, the sweet sorghum slag taking 1 part of 10kg is put in the glass container filling 10L dilute sulfuric acid, sweet sorghum slag is taken out after soaking 1h, extract water content front with immersion identical, place into the 2nd part of 10kg sweet sorghum slag, supplement 250g concentrated sulphuric acid simultaneously, soak 1h, extract front with immersion identical；It is then placed in the 3rd part of 10kg sweet sorghum slag, carries out the 2nd part of same operation, until all soaking complete.Such operation can reach 2 purposes: (1) supplements enough acid according to the moisture in sweet sorghum slag before soaking sweet sorghum slag every time makes acid concentration in the front acid solution of immersion every time identical；(2) along with soaking number of times increase, can penetrate into several times in acid solution before sugar remaining in sweet sorghum slag, but increase along with soaking number of times, when the sugared concentration of the sugared concentration soaked in pickle sorgo juice remaining with sweet sorghum slag is equal, no longer permeate in acid solution, fermentable sugars in sweet sorghum slag can be kept not lose；(3) anti-corrosion function of acid solution can make the sugar of remaining in sweet sorghum slag not lose when long-term preservation.Sweet sorghum slag natural air drying after pickling is 15wt% to water content, stacks storage.
Embodiment 4 sweet sorghum slag dilute acid steam explosion pretreatment
Take the air-dry sweet sorghum slag of pickling and carry out steam explosion pretreatment, boiling temperature 190 DEG C, digestion time 5min, burst pressure 0.8MPa.Moisture 33wt% in sweet sorghum slag butt, xylose 10wt%, glucose 5wt%, fructose 4wt%, content of cellulose 34.5wt%, half fiber content 2.6wt% after steam explosion pretreatment.
Embodiment 5 sweet sorghum slag enzymolysis and fermentation
5L fermentation tank is utilized to carry out sweet sorghum slag pretreatment and sweet sorghum slag and molasses fermented production ethanol test.Sweet sorghum slag add in NaOH and after, add according to batch feeding mode and carry out enzymolysis, initial feed addition is 1kg, then adds 1.25kg and 1.25kg sweet sorghum slag at twice respectively at 16h and 24h, and the solid content of enzymatic hydrolysis system is 28.9wt%.The addition of cellulase is 32IU/g cellulose (raw 1300 cellulase in pool), and enzymatic hydrolysis condition is: pH is 5.5, and temperature is 50 DEG C, and stir speed (S.S.) is 300rpm, and enzymolysis time is 24h.After enzymolysis, the concentration of glucose in enzymolysis solution is 12.4wt%, and xylose concentration is 4.1wt%.
Molasses are joined producing fuel ethyl alcohol by ferment in sweet sorghum slag enzymolysis solution in the ratio of 8.0wt%；The ethanol that fermentation is produced is easily separated, it is thus achieved that process of alcohol products.Concrete operations are: cool to 40 DEG C, disposable addition sugar grass molasses and 6% resistant to elevated temperatures yeast starter liquid (FE-B, CN200910204295.3), continue fermentation in 40 DEG C.During fermentation 115h, the concentration of alcohol in fermentation liquid can reach 10.3v%, and the glucose yield of sweet sorghum slag cellulase solution is 65wt%, and glucose fermentation is the 90wt% that yield is theoretical value of ethanol.
Embodiment 6 sweet sorghum slag enzymolysis and fermentation
Sweet sorghum slag add in NaOH and after, batch feeding adds and carries out enzymolysis, and initial feed addition is 1kg, then adds 0.75kg and 0.25kg sweet sorghum slag at twice respectively at 16h and 24h, and the solid content of enzymatic hydrolysis system is 16.5wt%.The addition of cellulase is 32IU/g cellulose (raw 1300 cellulase in pool) enzymatic hydrolysis condition: pH is 5.5, and temperature is 50 DEG C, and stir speed (S.S.) is 200rpm, and enzymolysis time is 24h.After enzymolysis, the concentration of glucose in enzymolysis solution is 8.4wt%, and xylose concentration is 2.0wt%.
Molasses are joined producing fuel ethyl alcohol by ferment in sweet sorghum slag enzymolysis solution in the ratio of 15.8wt%；The ethanol that fermentation is produced is easily separated, it is thus achieved that process of alcohol products.Concrete operations are: cool to 40 DEG C, disposable addition sugar grass molasses and and 6% resistant to elevated temperatures yeast starter liquid (FE-B, CN200910204295.3), in 40 DEG C continue fermentation.During diastatic fermentation 115h, the concentration of alcohol in fermentation liquid can reach 10.3v%, and the glucose yield of sweet sorghum slag cellulase solution is 80wt%, and glucose fermentation is the 90wt% that yield is theoretical value of ethanol.
Claims (14)
1. the method that sugar grass prepares alcohol fuel, comprises the following steps:
(1) the fresh bar of sugar grass is squeezed, it is achieved juice, slag separate；
(2) the sugar grass syrup obtained concentrates, and prepares into molasses；
(3) sweet sorghum slag obtained is pulled out after utilizing dilute acid soln immersion and is dried；
(4) sweet sorghum slag that step (3) obtains carries out dilute acid pretreatment or dilute acid steam explosion pretreatment；
(5) pretreated sweet sorghum slag add in alkali and after, add cellulase degradation, the solid content of enzymatic hydrolysis system is 5wt%-35wt%, obtains sweet sorghum slag enzymolysis solution after enzymolysis；
(6) molasses are joined producing fuel ethyl alcohol by ferment in sweet sorghum slag enzymolysis solution in the ratio of 0-30wt%；
(7) ethanol that fermentation is produced is easily separated, it is thus achieved that process of alcohol products.
2. in accordance with the method for claim 1, it is characterized in that: sugar grass can defoliation before squeezing the juice, also can not directly squeeze by defoliation, squeeze pressure is 30-60MPa, crushing juice rate is 60wt%-85wt%, in sweet sorghum juice, sugar content is 10wt%-20wt%, and in sweet sorghum slag, content of cellulose is 30wt%-40wt%, and hemicellulose level is 20wt%-30wt%.
3. in accordance with the method for claim 1, it is characterised in that: sugar grass syrup need to concentrate the molasses that saccharogenesis concentration is 40wt%-60wt%, and the method for concentration includes multiple-effect evaporation or membrane filtration.
4. in accordance with the method for claim 1, it is characterized in that: the sweet sorghum slag that water content is 10wt%-50wt% is placed in the dilute acid soln of 2wt%-20wt% and soaks 0.5-24h, then the sweet sorghum slag of pickling being pulled out from diluted acid, drip-dry moisture, natural air drying is 10wt%-30wt% to water content.
5. in accordance with the method for claim 1, it is characterised in that: the condition of dilute acid pretreatment is acid concentration 0.1wt%-5.0wt%, solid content 5wt%-35wt%, temperature 120-200 DEG C, processes time 0.5-5.0h.
6. in accordance with the method for claim 1, it is characterised in that: dilute acid steam explosion pretreatment condition is temperature 140-200 DEG C, digestion time 3-10min, pressure 0.5-0.9MPa.
7. in accordance with the method for claim 1, it is characterised in that: moisture 5wt%-40wt%, xylose 5wt%-20wt% in the sweet sorghum slag butt obtained after pretreatment, glucose 1wt%-10wt%, fructose 3wt%-5wt%, cellulose 25wt%-45wt%, hemicellulose 0.5wt%-21wt%.
8. in accordance with the method for claim 1, it is characterised in that: cellulase addition is 5-50IU/g cellulose, and enzymatic hydrolysis condition is: pH is 4.5-5.5, and temperature is 45-55 DEG C, and stir speed (S.S.) is 5-300r/min, and enzymolysis time is 24-120h.
9. the method described in claim 1 or 8, it is characterised in that: the solid content of enzymatic hydrolysis system is 5wt%-20wt%.
10. in accordance with the method for claim 1, it is characterised in that: it is directly added into molasses without solid-liquid separation after enzymolysis, carries out band slag fermentation.
11. in accordance with the method for claim 1, it is characterised in that: after enzymolysis, the concentration of glucose in enzymolysis solution is 2wt%-15wt%, and xylose concentration is 1wt%-6wt%.
12. in accordance with the method for claim 1, it is characterised in that: the sugared concentration of fermentation liquid is 15wt%-25wt%, and the additional proportion of molasses is 10wt%-30wt%.
13. in accordance with the method for claim 1, it is characterised in that: alcohol fermentation includes alcohol fermentation bacterium seed culture or activation, and switching and the fermentation of alcohol fermentation bacterium generate ethanol.
14. the method described in claim 1 or 13, it is characterised in that: alcohol fermentation bacterium seed culture or activation include the molasses to be diluted to sugar content 1wt%-5wt% as culture matrix.
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| CN109306362A (en) * | 2017-07-27 | 2019-02-05 | 重庆恒远晋通科技有限公司 | A method of based on water alcohol oxidizing process preparing ethyl alcohol from sweet sorghum |
| CN108374024B (en) * | 2018-02-12 | 2021-10-26 | 北海明世科技开发有限公司 | Method for co-producing ethanol, fructose and various byproducts by using sweet sorghum stalks and corns |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101220379A (en) * | 2008-02-05 | 2008-07-16 | 中国农业大学 | Method for producing ethyl alcohol by using sorgo stalk |
| WO2009148945A1 (en) * | 2008-05-29 | 2009-12-10 | Danisco Us Inc., Genencor Division | Process for alcohol and co-product production from grain sorghum |
| CN102154380A (en) * | 2010-12-20 | 2011-08-17 | 安徽丰原发酵技术工程研究有限公司 | Method for preparing ethanol from sweet sorghum stalks |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101220379A (en) * | 2008-02-05 | 2008-07-16 | 中国农业大学 | Method for producing ethyl alcohol by using sorgo stalk |
| WO2009148945A1 (en) * | 2008-05-29 | 2009-12-10 | Danisco Us Inc., Genencor Division | Process for alcohol and co-product production from grain sorghum |
| CN102154380A (en) * | 2010-12-20 | 2011-08-17 | 安徽丰原发酵技术工程研究有限公司 | Method for preparing ethanol from sweet sorghum stalks |
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