CN103767888A - Application of enteromorpha polysaccharide in preparation of cosmetic additive - Google Patents
Application of enteromorpha polysaccharide in preparation of cosmetic additive Download PDFInfo
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- CN103767888A CN103767888A CN201410056366.0A CN201410056366A CN103767888A CN 103767888 A CN103767888 A CN 103767888A CN 201410056366 A CN201410056366 A CN 201410056366A CN 103767888 A CN103767888 A CN 103767888A
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Abstract
The invention provides application of enteromorpha polysaccharide in preparing a cosmetic additive. The enteromorpha polysaccharide can be used as an absorbent, a humectant and a human skin fibroblast protective agent in cosmetics. Experiments and researches discover that enteromorpha polysaccharide has excellent absorption and moistening activities, has a good protective effect on skin cells, and in particular can remarkably improve the cell survival rate under a drying condition, so that the enteromorpha polysaccharide can be applied to cosmetics as humectant and absorbent.
Description
Technical field
The invention belongs to sea grass polysaccharide technical field, relate in particular to sea grass polysaccharide in the application for the preparation of in cosmetics additive.
Background technology
Skin is one natural cover for defense of human body, and moisture absorption, moisturizing are to maintain the normal key factor of this road barrier function.Healthy skin is smooth, soft and resilient, and the moisture in keratodermatitis is to maintain skin softness and elastic most important factor.While only having moisture sufficient, cell in skin histology just can carry out normal substance metabolism, maintain normal organizational structure and physiological function, externally can resist the invasion and attack of all undesirable elements, as stimulation of sun exposure, chemical substance etc., internally can keep nutrient substance and moisture abundance in cell, thereby guarantee the stable of organismic internal environment, guarantee the moist glossy of skin.On the market the cosmetics of selling mostly by supplementing important oiliness composition, hydrophilic moisturizing composition, and bring other active component into reach the object of moisturizing as carrier, make skin moisturizing, health.In cosmetics, comparatively conventional material which can retain moisture mainly contains polyalcohols (propylene glycol etc.), polysaccharide (hyaluronic acid etc.) at present, and ceramide type etc.
Entermorpha is a kind of large-scale economic chlorella, is rich in the physiologically active ingredients such as multiple polysaccharide, protein, vitamin and mineral.Modern study shows, sea grass polysaccharide has good blood fat reducing, antiviral, improves the biological activitys such as immunity.At present, there is no sea grass polysaccharide as additive application in the report of cosmetic industry.
Summary of the invention
The object of the present invention is to provide sea grass polysaccharide in the application for the preparation of in cosmetics additive, the present invention the experiment proved that sea grass polysaccharide has hygroscopicity and moisture retention, and originate safety, Nantural non-toxic pair, therefore can be applied to cosmetics additive.
For achieving the above object, the technical solution adopted in the present invention is:
The invention provides sea grass polysaccharide in the application for the preparation of in cosmetics additive.
Described sea grass polysaccharide is as the hygroscopic agent in cosmetics, wetting agent and human skin fibroblast protective agent.
The preparation method of wherein said sea grass polysaccharide is:
1) pretreatment of Entermorpha: be below 10% by the moisture removal in fresh Entermorpha to water content, pulverize, Entermorpha powder sieves to obtain;
2) extraction of sea grass polysaccharide: get described Entermorpha powder, add and account for Entermorpha powder quality 10-15 water doubly, add again the compound enzyme that accounts for Entermorpha powder quality 5%-8%, regulatory enzyme enzymatic hydrolysis system PH is between 4.0-6.0, keeping enzymatic hydrolysis system temperature is between 40-55 ℃, enzymolysis 4-6 hour, filters to obtain sea grass polysaccharide extracting solution;
3) extracting solution is concentrated: concentrate and make the polyoses content of described sea grass polysaccharide extracting solution be increased to 6-10%, obtain sea grass polysaccharide concentrated solution;
4) separation and purification: get described sea grass polysaccharide concentrated solution, utilize DEAE gel column to carry out preliminary purification to Entermorpha crude polysaccharides, utilize sodium chloride solution gradient elution, merge and collect corresponding eluting peak, eluent passes through molecular sieve gel post, deionized water eluting, purification again, merge and collect eluting peak, lyophilization is sea grass polysaccharide described in gained.
Further improvement to technique scheme: described step 2) described in compound enzyme be selected from one or more in cellulase, polysaccharide degrading enzyme, protease, the enzyme work of compound enzyme is 3 × 10
4-5 × 10
4iU.
Further improvement to technique scheme: in described step 3), the concentrated ultrafilter membrane molecular weight adopting is 300-1000D.
Further improvement to technique scheme: in described step 4), DEAE gel column is DEAE-Sepharose FF gel column or DEAE-Cellulose anion-exchange chromatography post.
Further improvement to technique scheme: in described step 4), concentration of sodium chloride solution is 0-0.2mol/L.
Further improvement to technique scheme: in described step 4), molecular sieve gel post is Sephadex G-10 gel column, Sephadex G-15 gel column or Sephadex G-25 gel column.
Further improvement to technique scheme: described sea grass polysaccharide is when as human skin fibroblast protective agent, and the mass concentration of its addition is 0.2-0.8%.
Compared with prior art, advantage of the present invention and beneficial effect are:
(1) raw material sources are abundant, safe.Entermorpha is as the large-scale economic chlorella of one, and natural propagation is very competent, and output is huge, and China marine site particularly Area of The East China Sea resource is extremely abundant.And the sea grass polysaccharide therefrom extracting has good biocompatibility, has no side effect, safe to use.
(2) sea grass polysaccharide has certain viscosity and good film property, under drying condition, can form the special protecting film of one deck at cell surface, thereby reduces the evaporation of skin surface moisture, and protection Skin Cell is unlikely impaired.Experimental result demonstration, sea grass polysaccharide has good hygroscopicity and moisture retention.In addition, sea grass polysaccharide can effectively prevent that under drying regime human skin fibroblast is damaged, and significantly improves cell survival rate.
The specific embodiment
Below in conjunction with the specific embodiment, technical scheme of the present invention is described in further detail.
Hygroscopicity effect experiment of embodiment 1, sea grass polysaccharide
The preparation method of sea grass polysaccharide of the present invention comprises the following steps:
1, Entermorpha pretreatment
Because breaking out of Entermorpha has very strong seasonality, effectively preserve Entermorpha the season of therefore breaking out in a large number at Entermorpha, for the carrying out of follow-up test, seems particularly important.Concrete processing method is: adopt the method described in patent " ZL200910313001.0 Entermorpha method for innocent treatment and equipment thereof " to process, specific as follows: the fresh Entermorpha extrusion dehydration baling equipment of just salvaging disembarkation is carried out to extrusion dehydration, slough the free water of 20-30%, be down to 60-70% containing water content, volume-diminished is original 1/4～1/5; By Entermorpha fragmentation, discharge the free water in Entermorpha tubular structure with cutter, then carry out secondary extrusion dehydration, make water content be down to 50%, now utilize hot blast to carry out pneumatic conveying drying dehydration, water content is down to below 10%, utilize the cyclone separator roguing of removing sand; Finally enter pulverizer and pulverize, so far realize the harmless treatment of Entermorpha, obtain Entermorpha powder after 60-80 mesh sieve.
2, the extraction of sea grass polysaccharide
The present embodiment is by the method for enzymolysis, extract sea grass polysaccharide, concrete step is: get the 100g of Entermorpha powder described in step 1, add the water of 1000ml-1500ml volume, add 5g-8g compound enzyme, comprise one or more in cellulase, polysaccharide degrading enzyme, protease, regulation system PH is between 4.0-6.0, keeping system temperature is between 40-55 ℃, and enzyme work is 3 × 10
4-5 × 10
4iU, enzymolysis 4-6 hour, plate-and-frame filtration, abandons filtering residue, obtains sea grass polysaccharide extracting solution, and the sea grass polysaccharide content of measuring this extracting solution is 2.5-4%, and the extraction ratio of sea grass polysaccharide is more than 80%.
Or the extraction of sea grass polysaccharide also can adopt following methods: get described Entermorpha powder, add and account for Entermorpha quality 15-20 water doubly, at 90-100 ℃ of hot water extraction 2-4 hour, filter.
3, extracting solution is concentrated
Concentrated is that extracting solution is processed, and the concentration of sea grass polysaccharide in solution is improved.For improving the sea grass polysaccharide content of extracting solution, the present invention has adopted concentration step to improve the concentration of enteromorpha extract, concrete grammar is: adopt membrane filtration, water is separated from enteromorpha extract, make the content of sea grass polysaccharide in sea grass polysaccharide extracting solution reach 6-10%.The ultrafilter membrane molecular weight adopting is 300-1000D, can effectively concentrate.
4, the separation and purification of sea grass polysaccharide
Get appropriate above-mentioned sea grass polysaccharide concentrated solution, upper DEAE gel column (can be DEAE-Sepharose FF gel column, DEAE-Cellulose anion-exchange chromatography post), Entermorpha crude polysaccharides is carried out to preliminary purification, and 0-0.2mol/L sodium chloride solution gradient elution, merges and collects corresponding eluting peak.Eluent is again by molecular sieve gel post (can be Sephadex G-10 gel column, Sephadex G-15 gel column, Sephadex G-25 gel column), and deionized water eluting, is further purified, and merges and collects eluting peak, and lyophilization is gained finished product.
Precision takes the above-mentioned appropriate sea grass polysaccharide making, and take glycerol, hyaluronic acid as contrast, puts into relative humidity and is respectively 80%, 40% constant temperature and humidity incubator, and set temperature is 25 ℃, starts experiment after temperature value in casing is stable.Accurate weighing after placement different time, according to the calculating hydroscopicity of poor quality before and after placing.
Hydroscopicity formula is as follows: hydroscopicity (%)=100 × (W
1-W
0)/W
0, wherein, W
1the weight of sea grass polysaccharide after expression moisture absorption, W
0the weight that represents the front sea grass polysaccharide of moisture absorption, unit is g.
As can be seen from Table 1, three kinds of materials are that hygroscopicity size order in 80% environment is: hyaluronic acid > glycerol > sea grass polysaccharide at relative humidity, and in initial 12h, the hydroscopicity of each sample all increases fast along with time lengthening, and after 12h, three's hydroscopicity speedup slows down gradually.And in the environment that is 43% at relative humidity, the hygroscopicity size order of three kinds of materials is: sea grass polysaccharide > glycerol > hyaluronic acid.Along with the reduction of ambient humidity, the hydroscopicity of three kinds of materials all shows downward trend, and glycerol and hyaluronic hydroscopicity decline more obviously compared with sea grass polysaccharide, and experimental result shows, under low relative humidity environment, the moisture sorption effect of sea grass polysaccharide is more remarkable.
The hydroscopicity (25 ℃) of three kinds of materials of table 1 under two kinds of humidity
Moisture retention effect experiment of embodiment 2, sea grass polysaccharide
Accurately take and utilize the appropriate sea grass polysaccharide sample that method makes described in embodiment 1, after adding appropriate distilled water fully to mix, be placed in the exsiccator that dry silica gel is housed, contrast adopts glycerol and hyaluronic acid, and accurate weighing after different time calculates moisturizing rate.
Moisturizing rate (%)=100 × W
1/ W
0
Wherein, W
1for placing rear wet weight, W
0for placing front wet weight, unit is g.
As can be seen from Table 2, along with time lengthening, the moisturizing rate continuous decrease of each material, and the speed that glycerol declines is much larger than other two kinds of materials.Performance of keeping humidity is arranged as from high to low: hyaluronic acid > sea grass polysaccharide > glycerol.Sea grass polysaccharide can form gelation space network in aqueous solution, can effectively stop the release of hydrone, shows good performance of keeping humidity.
Performance of keeping humidity (%) parameter of three kinds of materials of table 2
Cytoprotective effect experiment of embodiment 3, sea grass polysaccharide
The present embodiment has been observed the cytoprotection situation of sea grass polysaccharide to human skin fibroblast.Adopt conventional mtt assay to measure.Before experiment, need cell inoculum density to screen.Secondly, observe the protective effect of sea grass polysaccharide to human skin fibroblast: get proper density cell and be laid in 96 orifice plates, establish parallel control group, medicine group (basic, normal, high dosage).Cultivate after 48h, change culture medium and continue to cultivate.Treat that cell enters logarithmic growth after date, add respectively variable concentrations sea grass polysaccharide, continue to cultivate 12h.Then remove all culture medium, under dry environment, continue to cultivate after 4h, measure the activity of cell Mitochondria with mtt assay, calculate cell survival rate (%).
As can be seen from Table 3, while cultivation under drying condition, the human skin fibroblast survival rate that gives sea grass polysaccharide processing is improved significantly, and the sea grass polysaccharide concentration of increase rate and interpolation is proportionate.
The impact of table 3 sea grass polysaccharide addition on cell survival rate (%)
Above embodiment is only in order to technical scheme of the present invention to be described, but not is limited; Although the present invention is had been described in detail with reference to previous embodiment, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (9)
1. sea grass polysaccharide is in the application for the preparation of in cosmetics additive.
2. sea grass polysaccharide according to claim 1, in the application for the preparation of in cosmetics additive, is characterized in that: described sea grass polysaccharide is as the hygroscopic agent in cosmetics, wetting agent and human skin fibroblast protective agent.
3. sea grass polysaccharide according to claim 1 and 2, in the application for the preparation of in cosmetics additive, is characterized in that: the preparation method of described sea grass polysaccharide is:
1) pretreatment of Entermorpha: be below 10% by the moisture removal in fresh Entermorpha to water content, pulverize, Entermorpha powder sieves to obtain;
2) extraction of sea grass polysaccharide: get described Entermorpha powder, add and account for Entermorpha powder quality 10-15 water doubly, add again the compound enzyme that accounts for Entermorpha powder quality 5%-8%, regulatory enzyme enzymatic hydrolysis system PH is between 4.0-6.0, keeping enzymatic hydrolysis system temperature is between 40-55 ℃, enzymolysis 4-6 hour, filters to obtain sea grass polysaccharide extracting solution;
3) extracting solution is concentrated: concentrate and make the polyoses content of described sea grass polysaccharide extracting solution be increased to 6-10%, obtain sea grass polysaccharide concentrated solution;
4) separation and purification: get described sea grass polysaccharide concentrated solution, utilize DEAE gel column to carry out preliminary purification to Entermorpha crude polysaccharides, utilize sodium chloride solution gradient elution, merge and collect corresponding eluting peak, eluent passes through molecular sieve gel post, deionized water eluting, purification again, merge and collect eluting peak, lyophilization is sea grass polysaccharide described in gained.
4. sea grass polysaccharide according to claim 3 is in the application for the preparation of in cosmetics additive, it is characterized in that: described step 2) described in compound enzyme be selected from one or more in cellulase, polysaccharide degrading enzyme, protease, the enzyme work of compound enzyme is 3 × 10
4-5 × 10
4iU.
5. sea grass polysaccharide according to claim 3, in the application for the preparation of in cosmetics additive, is characterized in that: in described step 3), the concentrated ultrafilter membrane molecular weight adopting is 300-1000D.
6. sea grass polysaccharide according to claim 3, in the application for the preparation of in cosmetics additive, is characterized in that: in described step 4), DEAE gel column is DEAE-Sepharose FF gel column or DEAE-Cellulose anion-exchange chromatography post.
7. sea grass polysaccharide according to claim 3, in the application for the preparation of in cosmetics additive, is characterized in that: in described step 4), concentration of sodium chloride solution is 0-0.2mol/L.
8. sea grass polysaccharide according to claim 3, in the application for the preparation of in cosmetics additive, is characterized in that: in described step 4), molecular sieve gel post is Sephadex G-10 gel column, Sephadex G-15 gel column or Sephadex G-25 gel column.
9. sea grass polysaccharide according to claim 2, in the application for the preparation of in cosmetics additive, is characterized in that: described sea grass polysaccharide is when as human skin fibroblast protective agent, and the mass concentration of its addition is 0.2-0.8%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109044942A (en) * | 2018-08-27 | 2018-12-21 | 项佳红 | A kind of multiple plant skin care item and preparation method thereof for moisturizing |
| CN109090346A (en) * | 2017-06-21 | 2018-12-28 | 威海温喜生物科技有限公司 | Molten slurry of a kind of green alga and preparation method thereof |
| CN114376233A (en) * | 2022-03-23 | 2022-04-22 | 青岛海大生物集团股份有限公司 | Enteromorpha polysaccharide health food with immunity enhancing function and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898536A (en) * | 2012-10-19 | 2013-01-30 | 中国科学院海洋研究所 | Method for degrading enteromorpha polysaccharide |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN103396499A (en) * | 2013-08-14 | 2013-11-20 | 上海海洋大学 | Enteromorpha prolifera polysaccharide, and extraction method and application thereof |
-
2014
- 2014-02-19 CN CN201410056366.0A patent/CN103767888B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102898536A (en) * | 2012-10-19 | 2013-01-30 | 中国科学院海洋研究所 | Method for degrading enteromorpha polysaccharide |
| CN103044570A (en) * | 2013-01-25 | 2013-04-17 | 福建海兴保健食品有限公司 | Extracting process for efficiently extracting polysaccharide of enteromorpha |
| CN103396499A (en) * | 2013-08-14 | 2013-11-20 | 上海海洋大学 | Enteromorpha prolifera polysaccharide, and extraction method and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 史大华: "浒苔多糖对H_2O_2诱导的PC12细胞损伤的保护作用", 《中国生化药物杂志》 * |
| 吕海燕等: "浒苔多糖的酶法提取、纯化及初步结构鉴定", 《食品研究与开发》 * |
| 崔玉兰: "浒苔多糖的提取分离及结构表征", 《福建农林大学》 * |
| 徐大伦等: "纤维素酶法提取浒苔多糖的工艺条件", 《海洋渔业》 * |
| 肖宝石: "酶法提取浒苔多糖工艺优化的研究", 《食品与机械》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109090346A (en) * | 2017-06-21 | 2018-12-28 | 威海温喜生物科技有限公司 | Molten slurry of a kind of green alga and preparation method thereof |
| CN109044942A (en) * | 2018-08-27 | 2018-12-21 | 项佳红 | A kind of multiple plant skin care item and preparation method thereof for moisturizing |
| CN114376233A (en) * | 2022-03-23 | 2022-04-22 | 青岛海大生物集团股份有限公司 | Enteromorpha polysaccharide health food with immunity enhancing function and preparation method thereof |
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| CN103767888B (en) | 2016-07-06 |
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Address after: 266003 Shandong province Qingdao City fish Road No. 5 Applicant after: Qingdao Seawin Biotech Group Co., Ltd. Address before: 508 room 23, hi tech industry center, Ocean University of China, Hongkong East Road, Laoshan, Qingdao, Shandong 266071, China Applicant before: Qingdao Seawin Biotech Group Co., Ltd. |
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Address after: 266000 No. 127 Huizhiqiao Road, Qingdao High-tech Zone, Shandong Province Patentee after: Qingdao Haida biological Group Co.,Ltd. Address before: 266003 Shandong province Qingdao City fish Road No. 5 Patentee before: QINGDAO SEAWIN BIOTECH GROUP Co.,Ltd. |