CN103756776A - Method for extracting suaeda salsa seed oil by adopting deionized water - Google Patents

Abstract

A deionized water extraction method for Suaeda salsa seed oil; the operation is as follows: 1) Pulverization. Take Suaeda salsa seeds and grind them to 60-150 mesh; 2) Soak. Thoroughly mix the uniformly crushed Suaeda salsa seed powder and deionized water at a weight-to-volume ratio of 1:3 to 1:10, and soak; 3) Adjust the pH value to 4.0-6.0; 4) Pyrolysis and catalysis, adjust the The solid-liquid mixture after the pH is put into a water bath with a temperature of 40-65° C. for pyrolysis and catalysis for 1-5 hours; 5) Centrifugal separation. The solid-liquid mixture after pyrolysis and catalytic reaction is centrifuged at a speed of 4000-10000 rpm/min for 10-30 minutes; the upper oil layer after centrifugation is sucked out to obtain Suaeda salsa seed oil, which is the required Suaeda salsa seed oil. The invention has the advantages of high oil yield, good quality, simple and safe process, strong operability and low cost.

Description

A kind of deionized water extraction method of Suaeda salsa seed oil

technical field

The invention relates to the technical field of vegetable oil production, and relates to a technical method for developing high-quality health-care and edible oils using Suaeda salsa seed oil, in particular to a deionized water extraction method for Suaeda salsa seed oil.

Background technique

Suaeda salsa, also known as Artemisia salsa, seafood dish, Suaeda (Suaeda) is an annual herb true halophyte of Chenopodiaceae (Chenopodiaceae), which is distributed in Northeast, North, Northwest and coastal areas in my country, mainly growing Saline-alkaline deserts and semi-deserts such as coastal and lakesides, shallow flat depressions, or coastal tidal flats and other saline swamp environments. In addition, Suaeda salsa can absorb a large amount of soluble salts from the soil, significantly increase the total porosity of the soil, reduce soil bulk density, increase soil organic matter, alkaline nitrogen, available phosphorus, available potassium, and soil bacteria, actinomycetes, and fungi. It has obvious bioremediation effect on saline soil.

"Compendium of Materia Medica Supplements" records that Suaeda salsa is salty and cool, non-toxic, and has the effects of clearing away heat and eliminating accumulation. Suaeda salsa seeds are rich in protein, amino acid, fat, polysaccharide, vitamins and trace elements. The protein with a protein molecular weight of 55000Da accounts for about 50% of the total protein, and its water solubility is very good, so it is easily digested and absorbed. There are 18 kinds of amino acids, and 8 kinds of essential amino acids are rich in content, basically in line with the complete protein recommended by the United Nations Health Organization. Among them, the contents of valine, phenylalanine and tyrosine are particularly rich, which are 6.45 times and 1.65 times higher than the standard respectively. The vitamin E content is as high as 200mg/100g, and it is rich in various trace elements such as Se. At the same time, the fat content of Suaeda salsa seeds is between 20% and 36%, and the unsaturated fatty acid content is more than 90%, of which linoleic acid is 75.10%, oleic acid is 10.98%, linolenic acid is 4.37% and stearic acid is 1.37%. %, and these nutrients are essential substances to maintain the functions of the human body, and have positive effects on reducing blood sugar and blood pressure, dilating blood vessels, preventing and treating cardiovascular diseases, and enhancing human immunity. Moreover, linoleic acid and linolenic acid It is also essential fatty acid for the human body, which is the premise of important metabolites such as prostaglandin EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid), and plays an important role in maintaining human health and regulating physiological functions . Therefore, Suaeda salsa seed oil has broad development and application prospects as nutritional oil or health care oil, and can promote the development of Suaeda salsa planting industry, and then promote the bioremediation of saline-alkali land.

The traditional methods for producing vegetable seed oil are mainly mechanical pressing and solvent extraction. Mechanical treatment has a limited degree of damage to seed cells, and the oil yield is low. At the same time, the pressing method needs to roast the seed kernels. This heat treatment will cause severe denaturation of the protein and affect its further use value. Therefore, the cake after oil extraction cannot be efficient. Utilization, thereby causing the waste of plant protein resources; Although the solvent extraction method has a high oil yield and is very easy to realize large-scale industrial production, there are organic solvent residues in the oil and cake, and eating is extremely harmful to human health and is easy to pollute. Environment, therefore, there is an urgent need to develop a process for simultaneously extracting vegetable oil and vegetable protein from oilseeds, especially today as people's awareness of safety, greenness, nutrition and health care is gradually increasing, the level of deep processing of products should be improved, and the production of green products that meet human health Products have become the consensus of today's society. The biotechnology of supercritical CO ₂ fluid extraction and biological enzymatic hydrolysis to extract vegetable oil has become an emerging separation technology in today's society. Because it is based on the premise of realizing green production, it has attracted widespread attention. The vegetable oil extracted by supercritical CO ₂ fluid extraction technology and enzymatic hydrolysis technology has high oil yield, good quality, mild treatment conditions, low denaturation of cake protein after degreasing, good availability, and easy separation of oil and cake (slag). However, due to the high-pressure equipment investment and maintenance costs of supercritical _CO2 fluid extraction, small and medium-sized enterprises cannot apply it; at the same time, the enzyme preparations used for enzymatic hydrolysis are expensive, so they cannot be widely used, so Workers engaged in deep processing of agricultural and forestry products must devote themselves to the research of low investment, low cost, quick effect, simple process, strong operability, low energy consumption, low emission, and green technology.

Contents of the invention

The purpose of the present invention is to provide a deionized water extraction method of Suaeda salsa seed oil with high oil yield and excellent oil quality.

To achieve the above object, the technical solution adopted in the present invention is:

Select non-polluting Suaeda salsa seeds, remove the coating, grind into powder, mix with deionized water, soak, adjust the pH of the solid-liquid mixture, and then carry out pyrolysis catalytic reaction. When the Suaeda salsa seed oil is precipitated, carry out centrifugation, Finally, the clear oil is separated. The clear oil is refined to obtain pure nutrition and health Suaeda salsa seed oil.

A deionized water extraction method of Suaeda salsa seed oil can be operated as follows:

1) Grinding: Select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and crushing, so that the solid particles can pass through a 60-150 mesh screen to collect the powder;

2) Soaking: take Suaeda salsa seed powder and deionized water and mix them fully at a ratio of 1:3-1:10 (g/l) by weight to liquid, soak for 1-3 hours, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 4.0-6.0 with hydrochloric acid or citric acid with a concentration of 0.1-1.0%;

4) Pyrolysis and catalysis: place the solid-liquid mixture after adjusting the pH value in a water bath at a temperature of 40-65°C, and perform a pyrolysis and catalysis reaction for 1-5 hours;

5) Centrifugal separation: put the solid-liquid mixture after the pyrolysis catalytic reaction on the centrifuge, 4000-10000rpm/min, centrifuge for 10-30min, directly suck out the upper clear oil layer after centrifugation to obtain clear oil, which is the required Suaeda seed oil.

The clear oil obtained above is subjected to vacuum dehydration or vacuum freeze-drying to obtain Suaeda salsa seed oil.

The present invention has the following advantages:

1. The process has strong operability and low cost. The raw material does not need to be dried, and the clear oil can be obtained through pyrolysis and catalytic reaction through centrifugation, which consumes less energy; the oil and protein are separated at the same time, which shortens the process route; deionized water is used as a "solvent", which has a wide range of sources, reduces costs, and is non-flammable, Explosive danger, and reduce environmental pollution;

2. The process is simple and safe. The operation and reaction conditions of the present invention are mild and the cycle is short.

3. The oil quality is good. The oil of the invention has high purity, low phospholipid content, light color and good quality, and the product maintains the original color, fragrance and taste.

4. The application effect is good. The deionized water oil extraction process is special in the oil industry. It is separated by utilizing the different affinity of non-oily substances in oil to oil and water, and the different specific gravity of oil and water.

The present invention uses deionized water as a "solvent" to extract oil and protein from Suaeda salsa. In the cell, oil is distributed in the colloidal network structure of proteins and other substances in the form of extremely fine droplets. Therefore, auxiliary heating and pyrolysis conditions are used to activate its own enzymatic reaction, which is conducive to the loosening of cell tissue, to promote protein decomposition and emulsification, thereby catalyzing the free dissociation of oil, and then through gravity centrifugation to finally extract high-quality alkali The purpose of pomegranate oil. Oil extraction with deionized water is another new breakthrough following supercritical CO ₂ fluid extraction and aqueous enzyme extraction methods. Oil extraction with deionized water will have a profound impact on the oil industry.

5. Far-reaching significance. The present invention obtains the nutritious and healthy Suaeda salsa seed oil for maintaining product quality through the green production method, and the present invention has stepped into a new level for the green production of the deep processing of edible salt-tolerant plants, so it is useful for guiding the subsequent deep processing of salt-tolerant plants Green production has indeed played a role in attracting jade. The present invention produces and develops Suaeda salsa seed oil for nutrition and health care, which will be favored, and will create higher economic and social benefits for improving people's lives, promoting health, and earning foreign exchange through exports; thereby better benefiting mankind and benefiting in society.

Detailed ways

Example 1

A deionized water extraction method of Suaeda salsa seed oil can be operated as follows:

1) Grinding: Select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and grinding, so that the solid particles can pass through a 60-mesh sieve to collect the powder;

2) Soaking: fully mix Suaeda salsa seed powder and deionized water at a ratio of 1:3 (g/l) by weight to liquid, soak for 2 hours, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 4.0 with hydrochloric acid with a mass concentration of 1.0%;

4) Pyrolysis and catalysis: place the solid-liquid mixture after adjusting the pH value in a water bath at a temperature of 50°C, and perform a pyrolysis and catalysis reaction for 5 hours;

5) Centrifugal separation: put the solid-liquid mixture of pyrolysis catalytic reaction on the centrifuge, 4000rpm/min, and centrifuge for 30min; directly suck out the upper clear oil layer after centrifugal separation to obtain clear oil, which is the required Suaeda salsa seed oil;

6) Vacuum dehydration or vacuum freeze-drying of the obtained clear oil to obtain Suaeda salsa seed oil.

7) Calculation of oil yield: the oil yield is 21.58%.

Example 2

A deionized water extraction method of Suaeda salsa seed oil can be operated according to the following steps:

1) Grinding: select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and grinding, so that the solid particles can pass through a 100-mesh sieve to collect the powder;

2) Soaking: fully mix Suaeda salsa seed powder and deionized water at a ratio of 1:4 (g/l) by weight to liquid, soak for 1 hour, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 5.0 with citric acid with a mass concentration of 0.1%;

4) Pyrolysis and catalysis: place the solid-liquid mixture after adjusting the pH value in a water bath at a temperature of 55°C, and perform a pyrolysis and catalysis reaction for 3 hours;

5) Centrifugal separation: put the solid-liquid mixture after the pyrolysis catalytic reaction on the centrifuge, 5000rpm/min, and centrifuge for 20min; directly suck out the upper clear oil layer after centrifugal separation to obtain the clear oil, which is the required Suaeda salsa seed oil ;

6) Vacuum dehydration or vacuum freeze-drying of the obtained clear oil to obtain Suaeda salsa seed oil.

7) Calculation of oil yield: The oil yield is 22.64%.

Example 3

A deionized water extraction method of Suaeda salsa seed oil can be operated according to the following steps:

1) Grinding: select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and grinding, so that the solid particles can pass through a 120-mesh sieve to collect the powder;

2) Soaking: fully mix Suaeda salsa seed powder and deionized water at a ratio of 1:6 (g/l) by weight to liquid, soak for 2 hours, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 4.5 with hydrochloric acid with a mass concentration of 1.0%;

4) Pyrolysis and catalysis: place the solid-liquid mixture after adjusting the pH value in a water bath at a temperature of 45°C, and perform a pyrolysis and catalysis reaction for 4 hours;

5) Centrifugal separation: put the solid-liquid mixture of pyrolysis catalytic reaction on the centrifuge, 8000rpm/min, and centrifuge for 15min; directly suck out the upper clear oil layer after centrifugal separation to obtain clear oil, which is the required Suaeda salsa seed oil;

6) Vacuum dehydration or vacuum freeze-drying of the obtained clear oil to obtain Suaeda salsa seed oil.

7) Calculation of oil yield: the oil yield is 20.38%.

Example 4

A deionized water extraction method of Suaeda salsa seed oil can be operated according to the following steps:

1) Grinding: Select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and grinding, so that the solid particles can pass through a 140-mesh sieve to collect the powder;

2) Soaking: fully mix Suaeda salsa seed powder and deionized water at a weight-to-volume ratio of 1:10 (g/l), soak for 3 hours, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 6.0 with hydrochloric acid with a mass concentration of 1.0%;

4) Pyrolysis and catalysis: put the solid-liquid mixture after adjusting the pH value in a water bath with a temperature of 65°C, and perform a pyrolysis and catalysis reaction for 2 hours;

5) Centrifugal separation: put the solid-liquid mixture of pyrolysis catalytic reaction on the centrifuge, 10000rpm/min, and centrifuge for 10min; directly suck out the upper clear oil layer after centrifugal separation to obtain clear oil, which is the required Suaeda salsa seed oil;

6) Vacuum dehydration or vacuum freeze-drying of the obtained clear oil to obtain pure and refined Suaeda salsa seed oil.

7) Calculation of oil yield: the oil yield is 22.75%.

Example 5

A deionized water extraction method of Suaeda salsa seed oil can be operated as follows:

1) Grinding: select the Suaeda salsa seeds that have been removed from the coating and pour them into a grinder for grinding and grinding, so that the solid particles can pass through the 80-mesh sieve to collect the powder;

2) Soaking: fully mix Suaeda salsa seed powder and deionized water at a ratio of 1:5 (g/l) by weight to liquid, soak for 3 hours, and keep stirring;

3) Adjust the pH value: adjust the pH value of the soaked solid-liquid mixture to 5.5 with 0.1% citric acid;

4) Pyrolysis and catalysis: put the solid-liquid mixture after adjusting the pH value in a water bath with a temperature of 60°C, and perform a pyrolysis and catalysis reaction for 1 hour;

5) Centrifugal separation: put the solid-liquid mixture after the pyrolysis catalytic reaction on the centrifuge, 6000rpm/min, and centrifuge for 30min; directly suck out the upper clear oil layer after centrifugal separation to obtain the clear oil, which is the required Suaeda salsa seed oil;

6) Vacuum dehydration and vacuum freeze-drying of the obtained clear oil to obtain Suaeda salsa seed oil.

7) Calculation of oil yield: The oil yield is 23.89%. Among them, palmitic acid is 6.68%, palmitoleic acid is 3.45%, oleic acid is 14.06%, linoleic acid is 69.11%, linolenic acid is 4.21%, stearic acid is 2.07%, unsaturated fatty acid is as high as 91.17%, higher than organic solvent extraction and super Unsaturated fatty acid content extracted by critical CO ₂ fluid extraction (References: 1. Yu Haiqin, Zhang Tianzhu, Wei Chunyan, Li Zhijian. Research on the oil content and fatty acid composition of three species of Suaeda plant seeds. Northwest Botanical Journal. 2005, 25(10 ):2077-2082.; 2. Liu Renmin, Zhang Kun, Cui Qingxin. Supercritical CO ₂ fluid extraction and GC/MS analysis of Suaeda salsa seed oil. China Oils and Fats. 2003,28(3):42-45.).

8) Identification of physical and chemical properties: specific gravity 0.9235mg/ml, acid value 1.87mg KOH/g, iodine value 1.66g I/100g, saponification value 176.73mg KOH/g, peroxide value 2.24meq/Kg. Compared with the Soxhlet extraction method reported in the literature, the Suaeda salsa seed oil extracted by this method has lower specific gravity, acid value, saponification value, peroxide value and phospholipids, and higher iodine value (references: 2, Liu Renmin, Zhang Kun, Cui Qingxin. Supercritical CO ₂ fluid extraction and GC/MS analysis of Suaeda salsa seed oil. China Oils and Fats. 2003,28(3):42-45.; 3. Li Hongshan, Fan Yanxia. Suaeda salsa seed oil Characteristic analysis of extractant. CHINA OILS ANDFATS.2010,35(1):74-76.).

Claims (2)

1. a deionized water extraction method for common seepweed seeds oil, is characterized in that: operation as follows,

1) pulverize: select the fluffy seed of alkali of removing dressing to pour grinding in pulverizer into, pulverize, make solid particulate can pass through 60-150 eye mesh screen, collect powder;

2) ratio immersion: the fluffy powder of seeds of alkali be take to feed liquid weightmeasurement ratio as 1:3-1:10(g/l with deionized water) is fully mixed, and soaks 1-3h, and constantly stirs;

3) regulate pH value: the hydrochloric acid that is 0.1-1.0% by mass concentration by the solidliquid mixture of immersion or lemon acid for adjusting pH value are to 4.0-6.0;

4) pyrolysis catalytic: be the water-bath of 40-65 ℃ by regulating solidliquid mixture after pH value to be placed in temperature, pyrolysis catalytic reacts 1-5h;

5) centrifugation: the reacted solidliquid mixture of pyrolysis catalytic is placed on whizzer to 4000-10000rpm/min, centrifugal 10-30min; By the direct sucking-off of upper strata boiled oil layer after centrifugation, obtain edible vegetable oil, be required common seepweed seeds oil.

2. according to the deionized water extraction method of common seepweed seeds oil described in claim 1, it is characterized in that: obtained edible vegetable oil is obtained to common seepweed seeds oil through vacuum hydro-extraction or vacuum freeze-drying method.