CN103743916B - A fully automated chemiluminescence immunoassay analyzer - Google Patents

A fully automated chemiluminescence immunoassay analyzer Download PDF

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CN103743916B
CN103743916B CN 201310718774 CN201310718774A CN103743916B CN 103743916 B CN103743916 B CN 103743916B CN 201310718774 CN201310718774 CN 201310718774 CN 201310718774 A CN201310718774 A CN 201310718774A CN 103743916 B CN103743916 B CN 103743916B
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CN 201310718774
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CN103743916A (en )
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不公告发明人
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江苏泽成生物技术有限公司
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Abstract

本发明采用一种全自动化学发光免疫分析仪,包括TIP管模块、反应管模块、样品管模块、试剂管模块、TIP管取放装置、三维机械臂模块,其中三维机械臂模块可以带动TIP管取放装置在工作平面上任意运动,反应管模块中的反应管用TIP管来加样品和加试剂,所述反应管由TIP管取放装置来取放和移动。 The present invention employs a fully automated chemiluminescence immunoassay analyzer module comprising TIP tube, the reaction tube module, the sample tube module, the reagent tube module, pick and place apparatus TIP tube, a three-dimensional manipulator module, wherein the module can be driven by a three-dimensional manipulator tube TIP means any movement to take place on the working plane, the reaction tube module to add to the reaction tube sample tube TIP and processing agents TIP tube by the tube pick and place device and pick and place movement of the reaction. 本发明的有益效果是:该方案使用TIP管取代针来加样和加试剂,避免了交叉污染,同时使用TIP头取放装置来替代机械手取放和移动反应管,这种方式的出错率要大大低于机械手方案。 Advantageous effect of the invention is: The program uses TIP substituted needle tube loading and processing agents, to avoid cross-contamination, while using pick and place head TIP alternative means and moving the robot and a reaction tube, the error rate in this manner for well below the robot program.

Description

一种全自动化学发光免疫分析仪 A fully automated chemiluminescence immunoassay analyzer

技术领域 FIELD

[0001] 本发明属于发光分析领域,具体涉及一种全自动化学发光分析仪的方案,以及一种应用于该方案的新型反应管。 [0001] The present invention belongs to the field of emission spectrometry, particularly relates to novel chemiluminescent reaction of a fully automated analyzer program, and a program applied to the tube.

背景技术 Background technique

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[0002]目前市场上绝大多数的全自动化学发光分析仪上都包括机械手或者链式反应管传送机构,还有样品针和试剂针这几个必不可少的组成模块。 [0002] The vast majority of the market on automated chemiluminescence analyzers reaction tube transport mechanism comprises a chain or robot, as well as the sample needle and needle these reagents now essential constituent modules. 这种技术方案是根据化学发光的原理及反应检测步骤来决定的,首先要将待测样品血清由样品针加入到反应管里,然后由试剂针抽取反应试剂加入到反应管中进行反应,之后需要机械手或者链式传送带把反应管移动到反应模块中进行升温反应,待完成反应后需要继续转移到清洗模块进行磁珠的清洗和吸附过程,最后进行检测。 This solution is based on the principle of chemiluminescence reaction detection step and determined, first of all to test sample from the sample needle serum was added to the reaction tube, the reaction reagent and the reagent extraction needle was added to the reaction tube to react, after need or chain conveyor robot is moved to the reaction tube for heating the reaction the reaction module, it is necessary to continue the reaction to be complete after the transfer module to the cleaning process for cleaning and adsorption of beads, and finally detected.

[0003] 在整个反应流程中反应管中的样品和试剂都需要用到针来抽取和加样,反应管的移动是需要靠机械手或者链式传动机构来完成。 [0003] throughout the reaction process in the reaction tube samples and reagents need to use a needle-like extraction and processing, is the need for movement of the reaction tube to complete the hand or by mechanical chain drive mechanism. 这两种方式都存在着缺陷,前者针加样存在着一个清洗问题,需要一套完整的清洗装置,包括栗,管路,清洗池,废液回收等,即使这样也存在着交叉污染的风险。 Both methods are flawed, the former there is a loading pin cleaning problems, it requires a complete cleaning apparatus comprising Li, pipes, cleaning pools, waste recycling, and even then there is the risk of cross-contamination . 而机械手或者链式传动的缺陷是结构复杂,故障率高。 The robot defect or chain drive is a complex structure, high failure rate.

[0004] 本发明设计了一种全自动化学发光免疫分析仪方案可以规避以上两种缺陷。 [0004] The present invention contemplates an automated chemiluminescence immunoassay analyzer embodiment can avoid the above two drawbacks.

发明内容 SUMMARY

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[0005] 本发明为了克服现有技术的不足,提供了一种全自动化学发光免疫分析仪,以及一种应用于该方案的特殊设计的反应管。 [0005] The present invention is to overcome the deficiencies of the prior art, to provide a fully automated reactor tube chemiluminescence immunoassay analyzer, and applied to a special design of the program.

[0006] 本发明采用一种全自动化学发光免疫分析仪,包括TIP管模块、反应管模块、样品管模块、试剂盒模块、TIP管取放装置、三维机械臂模块,其中三维机械臂模块可以带动TIP管取放装置在工作平面上任意运动,反应管模块中的反应管用TIP管来加样品和加试剂,所述反应管由TIP管取放装置来取放和移动。 [0006] The present invention employs a fully automated chemiluminescence immunoassay analyzer module comprising TIP tube, the reaction tube module, the sample tube module, the reagent cartridge module, pick and place apparatus TIP tube, a three-dimensional manipulator module, wherein the three-dimensional manipulator modules TIP tube drive means to take place in arbitrary motion on a work surface, the reaction tube module to add to the reaction tube sample tube TIP and processing agents TIP tube by the tube pick and place device and pick and place movement of the reaction.

[0007] 进一步地,所述反应管内部的尺寸与标准TIP头内部尺寸一致。 [0007] Further, the interior of the reaction tube dimensions consistent with the standard TIP first internal dimension.

[0008] 进一步地,反应管的上管口的内径和下管口的内径分别与标准TIP管一致。 The inner diameter [0008] Further, the reaction tube inner diameter of the nozzle and the lower nozzle of the tube coincide with the standard TIP.

[0009] 进一步地,所述反应管的外部结构和尺寸满足机械手抓取的要求。 [0009] Further, the outer structure and the size of the reaction tube to satisfy the requirements of gripping manipulator.

[0010] 本发明的有益效果是:该方案使用TIP头(管)取代针来加样和加试剂,避免了交叉污染,同时使用TIP头取放装置来替代机械手取放和移动反应管,这种方式的出错率要大大低于机械手方案。 [0010] Advantageous effects of the present invention are: The program uses TIP head (tube) substituted needle loading and processing agents, to avoid cross contamination, use TIP head pick and place means to replace the robot pick and place movement of the reaction tube, which ways error rate to be much lower than the robot program.

附图说明 BRIEF DESCRIPTION

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[0011] 图1是本发明实施例的立体示意图; [0011] FIG. 1 is a perspective view of an embodiment of the present invention;

[0012]图2是本发明实施例的TIP管模块立体示意图; [0012] FIG. 2 is a tube module TIP embodiment of the present invention, a perspective schematic view;

[0013]图3是本发明实施例的样品管模块立体示意图; [0013] FIG. 3 is a sample tube module according to the present embodiment of the invention, a perspective schematic view;

[0014]图4是本发明实施例的试剂盒模块立体示意图; [0014] FIG. 4 is a perspective view of the module according to embodiment of the kit of the present invention;

[0015]图5是本发明实施例的反应管模块立体示意图; [0015] FIG. 5 is a tube reactor module according to the present invention, a perspective view of the embodiment;

[0016]图6是本发明实施例的三维机械臂模块立体示意图; [0016] FIG 6 is a three-dimensional manipulator module according to the present embodiment of the invention, a perspective schematic view;

[0017]图7是本发明实施例的试剂瓶架模块立体示意图; [0017] FIG. 7 is a perspective schematic view of a reagent bottle holder module according to the present embodiment of the invention;

[0018] 图8是本发明实施例的反应管架立体示意图; [0018] FIG. 8 is a perspective view of the embodiment of the reaction tube rack embodiment of the present invention;

[0019] 图9是本发明实施例的反应管剖面立体示意图; [0019] FIG. 9 is a cross-sectional view of the reaction tube embodiment of the present invention, a perspective schematic view of embodiment;

[0020] 图10是本发明实施例的反应管与标准tip管的剖面对比图。 [0020] FIG. 10 is a cross-sectional view of the reaction tube with the standard comparison chart tip tube of this embodiment of the present invention.

具体实施方式 detailed description

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[0021] 以下内容结合说明书附图对本发明的具体实施方式作详细说明。 [0021] The following description in conjunction with the accompanying drawings of specific embodiments of the present invention will be described in detail.

[0022] 参见图1,本发明的全自动化学发光免疫分析仪由TIP管模块1,反应管模块2,样品管模块3,试剂盒模块4,废料盒5,Y轴机械臂6,Z轴机械臂7,TIP管取放装置8,清洗针装置9,X轴机械臂10,检测孔11,外置清洗液及废液桶12,以及电控部分和PC控制端组成。 [0022] Referring to Figure 1, automated chemiluminescence immunoassay analyzer of the present invention consists of TIP tube module 1, module 2 the reaction tube, sample tube, module 3, module 4 kit, waste box 5, Y-axis robot arm 6, Z-axis robot arm 7, TIP tube pick and place apparatus 8, the needle cleaning means 9, 10, 11, and external washing liquid waste container 12, and the electronic control portion and the control terminal PC is detected X-axis manipulator hole components.

[0023]图2是TIP管模块,该模块由支撑脚13,支撑架16,标准TIP管架17,和200微升TIP管14,1毫升TIP管15组成。 [0023] FIG. 2 is a TIP tube module, which is supported by legs 13, support frame 16, the rack 15 consisting of TIP 17 standard, and 200 microliters of 14,1 ml tube TIP TIP tube.

[0024]图3是样品管模块,该模块由样品管架21,样品管20,样品管架盒19,光电传感器18组成。 [0024] FIG. 3 is a sample tube module, the module frame 21 from the sample tube, the sample tubes 20, sample tube racks cartridge 19, the photosensor 18 consisting of. 每次当样品管架21插入样品管架盒19的时候,光电传感器18会探测到插入信号,传给PC控制端,使得仪器随时掌握样品上架信息。 When each sample tube into the sample tube rack holder 21 when the cassette 19, the photosensor 18 can detect the insertion signal, transmitted to the control terminal PC, such that the instrument is ready to grasp the information added to the sample.

[0025] 图4,图7为试剂盒模块,它由试剂盒架22,试剂盒托盘23,混匀齿条24,混匀电机25,传动轮26组成。 [0025] FIG. 4, FIG. 7 is a module kit, the kit holder 22 which, kit tray 23, a rack 24 mix, mixing motor 25, the drive wheel 24 is formed. 试剂盒架22内含有试剂瓶44,缓冲液瓶43和磁珠瓶。 22 holder kit comprising a reagent bottle 44, buffer bottle 43 and the bottle bead. 最靠近内部的圆形试剂瓶的是磁珠瓶,它在使用时需要不停的旋转来保证瓶内的磁珠不沉淀,磁珠瓶底部带有齿轮45,与混匀齿条24配合,靠混匀电机25通过传动轮26带动混匀齿条24做直线往复运动保持磁珠瓶不停地旋转,达到磁珠混勾的目的。 Round reagent bottle is closest to the inside of the bottle bead, when it is used to guarantee the need to stop rotation of the bottle does not precipitate the beads, magnetic beads bottom flask with a gear 45, 24 and mix with the rack, by mixing motor 25 via a gear wheel 26 driven by kneading rack 24 linearly reciprocates a bottle holding spinning beads, magnetic beads mixed to achieve the purpose of the hook.

[0026] 图5,图8是反应管模块。 [0026] FIG 5, FIG 8 is a tube reactor module. 它由反应盘垫脚28,反应盘支架29,反应管架30,反应盘体31,和反应管27组成。 It pad pin 28 from the reaction disc, the reaction disc holder 29, frame 30 of the reaction tube, the reaction disk 31, and the composition of the reaction tube 27. 反应管27按规律排列在反应管架30上,然后整体放在反应盘体31上,反应盘体31带加热功能,确保试剂反应所需的温度环境。 The reaction tubes 27 are arranged according to the law on the frame 30 of the reaction tube, and the whole body on the reaction disk 31, reaction plate 31 with a heating function of the body, to ensure the required reagents temperature environment. 而反应管27采用特殊设计,可以用TIP管取放装置8直接插入抓取,从而避免了使用额外的机械手装置来运送反应管,反应管口内部46的尺寸与标准TIP头内部尺寸一致,反应管包括上管和下管,标准TIP头包括上管、下管和吸头部分,其中反应管的上管口的内径和下管口的内径与标准TIP管的一致,这样就可以像取放TIP头一样来取放反应管了,而反应管外部47的尺寸仍然满足一般机械手(本领域常规机械手)抓取尺寸要求,可以一管多用。 The reaction tube 27 with special design, can be used TIP tube pick and place device 8 directly into the crawler, thereby avoiding the use of an additional robotic device to convey the reaction tube, the interior of the reaction tube orifice size as a standard 46 coincides TIP first internal dimension, the reaction tube comprising a uniform inner diameter of the standard TIP tube spout upper and lower tubes, the standard TIP head comprises a tube, the tube and the tip portion, wherein the reaction tube inner diameter of the nozzle lower so that it can be like to take place TIP head to take place as the reaction tube, the outer dimensions of the reaction tube 47 while still satisfying the general robots (robot conventional in the art) to fetch size requirements may be more than one use.

[0027] 如图10所示,本发明设计的反应管口内部尺寸与标准Tip管(头)内部尺寸一致,这样就能兼容标准Tip管枪头,能够取放标准Tip管的装置就能取放本发明设计的反应管。 [0027] As shown, the present invention contemplates a reaction nozzle internal dimension consistent with the standard Tip tube 10 (first) internal dimensions, so that the tube can be compatible with standard Tip pipette tip, standard Tip tube apparatus can take place can take place reactor tube design of the present invention. 同时反应管的外形与普通反应管基本一致,这样也可以配合普通的机械手使用,这样一种反应管可以兼容两种取放装置。 While the outer shape of the reaction tube consistent with the ordinary reaction tubes, which can be used with conventional robots, a reaction tube can be compatible with both pick and place device.

[0028] 参见图6,为三维机械臂模块,包括X轴机械臂、Y轴机械臂和Z轴机械臂。 [0028] Referring to Figure 6, a three-dimensional manipulator module comprises an X-axis robot arm, Y-axis and Z-axis robot arm manipulator. 其中X轴机械臂由X轴圆轴32,X轴底板33,X轴电机34,X轴滑块35,X轴传动带37组成,Y轴机械臂由Y轴电机36,Y轴圆轴40,Y轴传动带39组成,Z轴臂由Z轴电机38,Z轴导轨41,Z轴传动带42组成。 Wherein the X-axis by a robot arm 32, base plate 33 is the X-axis, the X-axis X-axis motor 34 is a round shaft, an X-axis slide 35, the belt 37 composed of the X-axis, Y-axis robotic arm by a Y-axis motor 36, Y axis 40 round shaft, composition Y-axis drive belt 39, Z-axis by a Z-axis motor 38 is an arm, Z-axis guide rail 41 is, Z-axis drive belt 42 composition.

[0029] 工作原理:由X轴y轴z轴组成的三维运动臂可以带动TIP管取放装置8在工作平面上任意运动。 [0029] The working principle: the X-axis by a z-axis y-axis movement of the arm consisting of a three-dimensional motion can drive any TIP in the working plane of the device 8 to take place. 例如要让tip管取放装置8从反应管模块2中提取反应管到检测孔11处,则X轴和1轴先将TIP管取放装置8移动到反应管模块2上其中一个反应管所在位置坐标上方,然后Z轴带动做上下运动。 For example, to make the tube tip 8 pick and place means detecting hole 11 of the reaction tube 2 module extracted from the reaction tube, the X-axis and a tube axis TIP first pick and place the mobile device 8 to the reaction tube module wherein a reaction tube 2 where position coordinates of the above, then the Z-axis drive up and down movement. 由tip管取放装置8插入反应管中并提起,再由X轴y轴运动到检测孔11坐标处,Z轴带动tip管取放装置8向下运动,放入检测孔11内,然后tip管取放装置8会有一个顶出机构将反应管从上面卸下,完成整个取放动作。 Take place by the tip tube 8 is inserted into the reaction tube apparatus and lifted, then the y-axis motion detecting hole 11 to the X-axis coordinate, Z-axis drive tube tip downward movement means 8 take place, into the detecting holes 11, then tip the tube 8 have a pick and place apparatus ejecting mechanism removed from above the reaction tube, to complete the operation to take place.

[0030] 以上所述仅为本发明的较佳实施例而已,并不用于限制本发明,凡在本发明的精神和原则之内所做的任何修改,等同替换和改进等,均应包含在本发明的保护范围内。 [0030] The foregoing is only preferred embodiments of the present invention, but not intended to limit the present invention, where any modifications within the spirit and principle of the present invention, equivalent substitutions and improvements should be included in the within the scope of the present invention.

Claims (8)

  1. 1.一种全自动化学发光免疫分析仪,包括TIP管模块、反应管模块、样品管模块、试剂盒模块、TIP管取放装置、三维机械臂模块,其中三维机械臂模块可以带动TIP管取放装置在工作平面上任意运动,其特征在于,反应管模块中的反应管用TIP管来加样品和加试剂,所述反应管由TIP管取放装置来取放和移动; 所述反应管口内部的尺寸与标准TIP头内部尺寸一致,反应管包括上管和下管; 所述反应管的外部结构和尺寸满足机械手抓取的要求,所述反应管的管口外部设有环形槽。 An automated chemiluminescence immunoassay analyzer module comprising TIP tube, the reaction tube module, the sample tube module, the reagent cartridge module, pick and place apparatus TIP tube, a three-dimensional manipulator module, wherein the module can be driven by a three-dimensional manipulator tube taken TIP any movement in the working plane discharge apparatus, characterized in that the reaction tube module to add to the reaction tube sample tube TIP and processing agents TIP tube by the tube pick and place device and pick and place movement of the reaction; the reaction spout inside the same size as a standard size TIP head inside the reaction tube comprises an upper and lower tubes; outer nozzle and the outer dimensions of the reaction tube structure satisfies the requirements of gripping manipulator, the reactor tube is provided with an annular groove.
  2. 2.根据权利要求1所述的分析仪,其特征在于,反应管的上管口的内径和下管口的内径分别与标准TIP管一致。 The analyzer according to claim 1, wherein the inner diameter of the reaction tube and the inner diameter of the spout at the spout tube coincide with the standard TIP.
  3. 3.根据权利要求1所述的分析仪,其特征在于,所述三维机械臂模块包括X轴机械臂、Y轴机械臂和z轴机械臂,其中X轴机械臂包括X轴圆轴、X轴底板、X轴电机、X轴滑块和X轴传动带,Y轴机械臂包括Y轴电机、Y轴圆轴和Y轴传动带,z轴机械臂包括Z轴电机、Z轴导轨和Z轴传动带。 The analyzer according to claim 1, wherein said three-dimensional manipulator module comprises an X-axis robot arm, Y-axis and z-axis robot arm robot arm, wherein the X-axis X-axis manipulator comprises a round shaft, X axis plate, X-axis motor, X-axis slider and an X-axis drive belt, Y-axis manipulator includes a Y-axis motor, Y-axis circular-axis and Y-axis drive belt, z-axis manipulator comprises a Z-axis motor, Z axis guide rail, and the Z-axis drive belt .
  4. 4.根据权利要求1所述的分析仪,其特征在于,所述分析仪还包括废料盒、清洗针装置、外置清洗液及废液桶、电控部分和PC控制端。 4. The analyzer according to claim 1, wherein said analyzer further comprises a waste box, cleaning needle device, cleaning fluid and waste container external, electrically controlled section and a control terminal PC.
  5. 5.根据权利要求1所述的分析仪,其特征在于,所述TIP管模块包括支撑脚、支撑架、标准TIP管架、200微升TIP管和1毫升TIP管。 The analyzer according to claim 1, wherein said module comprises a TIP tube support feet, the support frame, the standard TIP pipe rack, 200 microliters tube and 1 ml of TIP TIP tube.
  6. 6.根据权利要求1所述的分析仪,其特征在于,所述样品管模块,包括样品管架、样品管、样品管架盒和光电传感器。 The analyzer according to claim 1, wherein said sample tube module, comprising a sample tube holder, sample tube and the sample tube holder cartridge photosensor.
  7. 7.根据权利要求1所述的分析仪,其特征在于,试剂盒模块包括试剂盒架、试剂盒托盘、混匀齿条、混匀电机和传动轮,其中试剂盒架内含有试剂瓶、缓冲液瓶和磁珠瓶。 The analyzer according to claim 1, wherein the kit comprises a module holder kit, the kit tray, rack mixing, kneading motor and drive wheel, wherein the kit comprises a reagent bottle holder, buffer liquid bottle and beads bottle.
  8. 8.根据权利要求1所述的分析仪,其特征在于,所述反应管模块包括反应盘垫脚、反应盘支架、反应管架、反应盘体和反应管。 8. The analyzer according to claim 1, wherein said reaction tube includes a reaction disk module feet, the reaction disc holder, rack reaction tube, the reaction tube and the reaction tray.
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CN105353158B (en) * 2014-05-21 2017-02-22 凯和生物科技(上海)有限公司 An improved automatic immunoassay analyzer Article
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