CN103725602B - Thermal cover plate used in nucleic acid amplification detector - Google Patents
Thermal cover plate used in nucleic acid amplification detector Download PDFInfo
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- CN103725602B CN103725602B CN201210391428.4A CN201210391428A CN103725602B CN 103725602 B CN103725602 B CN 103725602B CN 201210391428 A CN201210391428 A CN 201210391428A CN 103725602 B CN103725602 B CN 103725602B
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- cover plate
- nucleic acid
- acid amplification
- upper cover
- press table
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a thermal cover plate used in a nucleic acid amplification detector. The thermal cover plate comprises an upper cover plate and a lower press plate which are horizontally and oppositely arranged, wherein the upper cover plate and the lower press plate are fixedly connected through at least two springs; at least two probes are fixedly mounted on the lower press plate; displacement sensors capable of quantitatively measuring the displacement of the probes relative to the upper cover plate are fixedly mounted on the upper cover plate; the number of the displacement sensors is equal to that of the probes and the mounting positions of the displacement sensors correspond to those of the probes; at least one heating component and at least one temperature sensor which is capable of detecting the temperature of the lower press plate are fixedly mounted on the lower press plate. The problem that the nucleic acid reaction can be failed as the pressure cannot be controlled due to the fact that the conventional nucleic acid amplification detector is lack of a device which can accurately control the pressure is solved, and the pressure and the temperature can be accurately controlled.
Description
Technical field
The present invention relates to nucleic acid amplification and detection field, specifically a kind of for the hot cover plate in the nucleic acid amplification detector of isothermal or temperature cycle.
Background technology
Nucleic acid samples will realize efficient, reproducible controlled chain reaction and need the reaction conditions that a temperature can fast, accurately change, because can repeat, the temperature of reaction of nucleic acid samples accurately in Quality control pond directly determines efficiency, the reliability of controlled chain reaction, be improve the sensitivity of nucleic acid amplification detector in clinical disease diagnosis application and specific key.Existing nucleic acid amplification detector, comprise isothermal or temperature cycle, instrument that qualitative or real time fluorescent quantitative detects, because the sample pool loading nucleic acid samples is placed in sample table, so the Quality control pond pressure be pressed in sample table directly determines the thermal conductivity between them, how accurately, efficiency and reproducibility that this pressure determines the reaction of nucleic acid samples repeatedly can be controlled.
A kind of Nucteic acid amplification gene chip hybridization detecting instrument is disclosed in Chinese patent CN00112248.7, integrate PCR, hybridization, cleaning, testing process, achieve the effect that automatic pointing detects automatic Output rusults, a kind of electric transducer for overdelicate detection of nucleic acids is disclosed in Chinese patent CN201080019103.2, in Chinese patent CN200420102062.5, disclose a kind of real-time fluorescence detecting device for nucleic acid amplification, provide detection sensitivity and accuracy by light source incidence in modifier and the fluorescent signal approach be excited.
But all lack the device that can be carried out accurate Repetitive controller pressure and temperature in existing nucleic acid amplification detector, like this when the mechanism of sample pool, size, material and foozle change time, final nucleic acid reaction error may be caused excessive even failed.
Summary of the invention
The object of this invention is to provide a kind of can accurately control pressure and temperature for the hot cover plate in nucleic acid amplification detector.
The technical scheme realizing the object of the invention is to provide a kind of for the hot cover plate in nucleic acid amplification detector, and described hot cover plate comprises the upper cover plate and press table that level is oppositely arranged, and is fixedly connected with between described upper cover plate and press table by least 2 springs; Described press table is installed with at least 2 probes, described upper cover plate is installed with can the displacement sensor of the relative upper cover plate displacement of quantitative measurment probe, institute's displacement sensors and number of probes quite and installation site and probe corresponding; Described press table is installed with the temperature sensor of the detectable press table temperature of at least 1 heater element and at least 1; By a force application apparatus can overall drive the upper cover plate of described hot cover plate, spring and press table along with the direction of the determined plane being perpendicular of upper cover plate on move up and down, described upper cover plate and press table stressed time do relative movement by spring.
Preferred described temperature sensor adopts RTD or thermistor or semiconductor transducer or thermopair.
Preferred described heater element is metal electric heating unit.
Preferred described upper cover plate is made of metal.
Preferred described upper cover plate is made up of nonmetal.
Preferred described press table is made of metal.
Preferred described press table is made up of nonmetal.
The material making press table described in preferred can stay active for long periods under the condition more than at least 100 degrees Celsius.
Preferred described force application apparatus is made up of motor and motor transmission mechanism.
Preferred described motor transmission mechanism is screw nut driven mechanism or Worm and worm-wheel gearing or belt wheel transmission mechanism or gear drive.
The present invention has positive effect: (1) is by the probe on press table, rate of travel between press table and upper cover plate can be reflected by the draught of spring, the displacement sensor be arranged on upper cover plate can record applied pressure size and namely calculate according to the draught of the coefficient of elasticity x spring of pressure=spring, like this by the pressure exactly between Quality control pond and sample table, and then in real time, compensate the mechanism of sample pool exactly, size, material and foozle, heat conducting consistence between Quality control pond and sample table, ensure final nucleic acid reaction efficiency and repeatability.
(2) heater element on press table and temperature sensor is arranged on, can the temperature at accurate top, Quality control pond, so that the temperature of reaction of inside, accurate perception Quality control pond, avoid reaction solution to condense in the top of sample pool simultaneously.
Accompanying drawing explanation
In order to make content of the present invention more easily be clearly understood, below according to specific embodiment also by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is a kind of structural representation for the hot cover plate in nucleic acid amplification detector;
1 is upper cover plate; 2 is press tables; 3 is springs; 4 is probes; 5 is displacement sensors; 6 is motors; 7 is heater elements; 8 is temperature sensors; 9 is motor transmission mechanisms; 10 is sample pools; 11 is sample table
Embodiment
See Fig. 1, the invention provides a kind of for the hot cover plate in nucleic acid amplification detector, described hot cover plate comprises the upper cover plate 1 and press table 2 that level is oppositely arranged, and is fixedly connected with between described upper cover plate 1 and press table 2 by least 2 springs 3; Described press table 2 is installed with at least 2 probes 4, described upper cover plate 1 is installed with can the displacement sensor 5 of relative upper cover plate 1 displacement of quantitative measurment probe 4, institute's displacement sensors 5 and installation site suitable with probe 4 quantity and probe 4 corresponding; Described press table 2 is installed with the temperature sensor 8 of detectable press table 2 temperature of at least 1 heater element 7 and at least 1; By a force application apparatus and motor 6 and motor transmission mechanism 9 can overallly drive the upper cover plate 1 of described hot cover plate, spring 3 and press table 2 along with the direction of the determined plane being perpendicular of upper cover plate 1 on move up and down, do relative movement by spring 3 when described upper cover plate 1 is stressed with press table 2.
Upper cover plate 1 is driven by motor 6 and motor transmission mechanism 9, spring 3 and press table 2 to be arranged on fixed for load and heated sample pond 10 sample table 11 on sample pool 10 apply pressure, the draught of the rate of travel=spring 3 wherein between press table 2 and upper cover plate 1, execute stressed size to record by the displacement sensor 5 be arranged on upper cover plate 1, namely calculate according to the draught of the coefficient of elasticity x spring of pressure=spring, like this can pressure exactly between Quality control pond 10 and sample table 11 by being arranged on displacement sensor 5 on upper cover plate 1, and then in real time, compensate the mechanism of sample pool 10 exactly, size, material and foozle, heat conducting consistence between Quality control pond 10 and sample table 11, ensure final nucleic acid reaction efficiency and repeatability.
Further described temperature sensor 8 adopts RTD or thermistor or semiconductor transducer or thermopair.
Further described heater element 7 is metal electric heating units.
By being arranged on heater element 7 on press table 2 and temperature sensor 8, can the temperature at accurate top, Quality control pond 10, so that the temperature of reaction of inside, accurate perception Quality control pond 10, avoid reaction solution to condense in the top of sample pool 10 simultaneously.
Further described upper cover plate 1 is by metal or nonmetally makes; Press table 2 is by metal or nonmetally makes, and the material making press table 2 can stay active for long periods under the condition more than at least 100 degrees Celsius.
Further described motor transmission mechanism 9 is screw nut driven mechanism or Worm and worm-wheel gearing or belt wheel transmission mechanism or gear drive.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. one kind for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described hot cover plate comprises the upper cover plate (1) and press table (2) that level is oppositely arranged, and is fixedly connected with between described upper cover plate (1) and press table (2) by least 2 springs (3); Described press table (2) is installed with at least 2 probes (4), described upper cover plate (1) is installed with can the displacement sensor (5) of relative upper cover plate (1) displacement of quantitative measurment probe (4), institute's displacement sensors (5) and installation site suitable with probe (4) quantity and probe (4) corresponding; Described press table (2) is installed with the temperature sensor (8) of the heater element (7) of at least 1 and detectable press table (2) temperature of at least 1; By a force application apparatus can overall drive the upper cover plate (1) of described hot cover plate, spring (3) and press table (2) along with the direction of upper cover plate (1) determined plane being perpendicular on move up and down, described upper cover plate (1) and press table (2) stressed time do relative movement by spring (3).
2. according to claim 1 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described temperature sensor (8) adopts RTD or thermistor or semiconductor transducer or thermopair.
3. according to claim 1 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described heater element (7) is metal electric heating unit.
4. according to any one of claim 1-3 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described upper cover plate (1) is made of metal.
5. according to any one of claim 1-3 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described upper cover plate (1) is made up of nonmetal.
6. according to claim 1 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described press table (2) is made of metal.
7. according to claim 1 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described press table (2) is made up of nonmetal.
8. according to claim 6 or 7 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described in make the material of press table (2) can stay active for long periods under the condition more than at least 100 degrees Celsius.
9. according to claim 1 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described force application apparatus is made up of motor (6) and motor transmission mechanism (9).
10. according to claim 9 for the hot cover plate in nucleic acid amplification detector, it is characterized in that: described motor transmission mechanism (9) is screw nut driven mechanism or Worm and worm-wheel gearing or belt wheel transmission mechanism or gear drive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210391428.4A CN103725602B (en) | 2012-10-16 | 2012-10-16 | Thermal cover plate used in nucleic acid amplification detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210391428.4A CN103725602B (en) | 2012-10-16 | 2012-10-16 | Thermal cover plate used in nucleic acid amplification detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103725602A CN103725602A (en) | 2014-04-16 |
| CN103725602B true CN103725602B (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210391428.4A Active CN103725602B (en) | 2012-10-16 | 2012-10-16 | Thermal cover plate used in nucleic acid amplification detector |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105302192A (en) * | 2014-05-31 | 2016-02-03 | 捷普科技（上海）有限公司 | Temperature control unit and biochip detection apparatus having the temperature control unit |
| CN105132283A (en) * | 2015-08-21 | 2015-12-09 | 杭州安杰思生物科技有限公司 | Hot lid mechanism of fluorescent quantitative PCR (polymerase chain reaction) instrument |
| CN105505761A (en) * | 2015-12-21 | 2016-04-20 | 中国科学院苏州生物医学工程技术研究所 | Digital isothermal nucleic acid detecting device and detecting method thereof |
| CN107866289A (en) * | 2016-09-23 | 2018-04-03 | 广州康昕瑞基因健康科技有限公司 | Metal bath heat lid |
| CN106987551B (en) * | 2017-03-08 | 2020-07-17 | 杭州柏恒科技有限公司 | Gene amplification instrument and control method |
| CN110069084A (en) * | 2018-01-24 | 2019-07-30 | 思纳福(北京)医疗科技有限公司 | Temperature control device |
| CN113546700B (en) * | 2021-05-21 | 2022-10-04 | 宁波胤瑞生物医学仪器有限责任公司 | Chip compressing device |
| CN114958580B (en) * | 2022-05-31 | 2023-01-13 | 山东省高精生物诊断分析产业技术研究院有限公司 | A hot lid structure for full-automatic nucleic acid analytic system |
| CN115651819B (en) * | 2022-09-20 | 2024-05-17 | 杭州准芯生物技术有限公司 | Self-adaptive jacking component for kit and detection device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6153426A (en) * | 1998-12-22 | 2000-11-28 | Mwg Biotech Ag | Thermocycler apparatus |
| EP1961484A1 (en) * | 2007-02-13 | 2008-08-27 | Eppendorf Ag | Cover for sample with homogeneous pressure application |
| CN201532192U (en) * | 2009-11-30 | 2010-07-21 | 哈尔滨科瑞精密仪器有限公司 | Large-range high-precision skeleton surveying displacement sensor |
| CN101952412A (en) * | 2008-02-15 | 2011-01-19 | 生物辐射实验室股份有限公司 | Thermal cycler with self-adjusting lid |
-
2012
- 2012-10-16 CN CN201210391428.4A patent/CN103725602B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6153426A (en) * | 1998-12-22 | 2000-11-28 | Mwg Biotech Ag | Thermocycler apparatus |
| EP1961484A1 (en) * | 2007-02-13 | 2008-08-27 | Eppendorf Ag | Cover for sample with homogeneous pressure application |
| CN101952412A (en) * | 2008-02-15 | 2011-01-19 | 生物辐射实验室股份有限公司 | Thermal cycler with self-adjusting lid |
| CN201532192U (en) * | 2009-11-30 | 2010-07-21 | 哈尔滨科瑞精密仪器有限公司 | Large-range high-precision skeleton surveying displacement sensor |
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| CN103725602A (en) | 2014-04-16 |
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