CN103720930B - A kind of Chinese medicine composition treating bovine mastitis - Google Patents

A kind of Chinese medicine composition treating bovine mastitis Download PDF

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CN103720930B
CN103720930B CN201310569091.6A CN201310569091A CN103720930B CN 103720930 B CN103720930 B CN 103720930B CN 201310569091 A CN201310569091 A CN 201310569091A CN 103720930 B CN103720930 B CN 103720930B
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ethanol
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CN103720930A (en
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赵桂霞
赵守财
何海军
侯琳琳
李�和
李博英
季文
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BEI'AN FEILONG ANIMAL PHARMACEUTICAL FACTORY
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Abstract

<b>disclosure</b><b>a kind of Chinese medicine composition treating bovine mastitis, is prepared from by 9 taste crude drug such as Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae, Herba Andrographis, Squama Manis, Moschus, Venenum Bufoniss.Said composition is containing multiple bioactive ingredients such as alkaloid, glycoside, organic acid, with feedstuff homology consubstantiality, easily absorb, have no side effect, there is heat-clearing and toxic substances removing, anti-inflammation, restore menstrual flow and invigorate blood circulation, subside a swelling and stop effect of carbuncle, active lactogenesis, and animal individual growth promoter can be promoted, strengthen animal immune ability.</b>

Description

A kind of Chinese medicine composition treating bovine mastitis
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of Chinese medicine composition treating bovine mastitis and preparation method thereof.
Background technology
In recent years, industry particularly milk cattle cultivating in rural culture develops rapidly, and raises people and scale is increasing/big.But, raise and lack standardized management, directly result in disease in raising dairy cattle occurred frequently.
Mastitis, including acute, chronic, hemorrhagic, suppurative, fungoid mastitis, are one of diseases most commonly seen in raising dairy cattle, prevalence nearly 1/3, clinical manifestation has: the milk in ill breast district goes out flocculus, and grumeleuse is sometimes in water sample, and breast has red, swollen, hot, pain shape in various degree;Body temperature raises, and pulse is accelerated, and spirit is depressed, and poor appetite or useless exhausted, milk yield is decline etc. rapidly.Milk quality and milk yield will be had a strong impact on as treated not in time, endanger the mankind and milk cow health.Currently, the multiplex antibiotic therapy of bovine mastitis, but effect is unstable, easily recurs, and be easily caused milk antibiotic and pollute, directly endanger human health.
Summary of the invention
It is an object of the invention to provide a kind of Chinese medicine composition treating bovine mastitis, the 9 taste crude drug such as Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae, Herba Andrographis, Squama Manis, Moschus, Venenum Bufonis be prepared from.Said composition is containing various bioactivators such as alkaloid, glycoside, organic acid, with feedstuff homology consubstantiality, easily absorb, have no side effect, there is heat-clearing and toxic substances removing, anti-inflammation, restore menstrual flow and invigorate blood circulation, subside a swelling and stop effect of carbuncle, active lactogenesis, and animal individual growth promoter can be promoted, strengthen animal immune ability.
The Chinese medicine composition of the treatment bovine mastitis of the present invention, is made up of component A and B component, and wherein, component A is prepared from by the crude drug of following weight portion:
Radix Bupleuri: 10 Herba Violaes: 50 Herba Taraxacis: 60
Flos Chrysanthemi Indici: 50 Fructus Forsythiaes: 30 Herba Andrographis: 50;
B component is prepared from by the crude drug of following weight portion:
Moschus: 0.8 Venenum Bufonis: 2 Squama Maniss: 40.
The preparation method of the Chinese medicine composition of above-mentioned treatment bovine mastitis, comprises the following steps:
One, Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae, Herba Andrographis, Squama Manis, Moschus, Venenum Bufonis 9 taste crude drug are cleaned, clean, dry, pulverize into coarse powder, weigh by weight, standby;
Two, the preparation of component A:
1) Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae and Herba Andrographis being added in extraction pot, inject the purified water of above-mentioned 6 taste 8-12 times of weight of medicine, heating, to boiling, decocts 3 hours, is leached by medicinal liquid, obtains first time filtrate, standby;
2) second time herb liquid extracts: in extraction pot, injects the purified water of above-mentioned 6 taste 6-10 times of weight of medicine, and heating, to boiling, decocts 2 hours, leached by medicinal liquid, obtains second time filtrate, standby;
3) third time herb liquid extracts: in extraction pot, injects the purified water of above-mentioned 6 taste 4-6 times of weight of medicine, and heating, to boiling, decocts 1 hour, leached by medicinal liquid, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container and be uniformly mixed, precipitation, filter, obtain filtrate, standby;
5) step 4 gained filtrate is concentrated with Chinese drug steaming device, be concentrated into 2/5ths of filtrate volume, obtain concentrated solution, standby;
6) step 5 gained concentrated solution is added the ethanol of the content 95% of 1 times of weight, carries out precipitate with ethanol 72 hours, then by separation of ethanol, obtain first time fluid extract;The ethanol of the content 95% that first time fluid extract is added 0.8 times of weight carries out precipitate with ethanol 48 hours, is then peeled off ethanol, obtains second time fluid extract;The ethanol of the content 95% that second time fluid extract is added 0.7 times of weight carries out precipitate with ethanol 24 hours, then again separation of ethanol, obtains the crude drug extractum prepared, i.e. component A;
Three, the preparation of B component:
1) Moschus, Venenum Bufonis and Squama Manis are added in ultrasonic extracting machine, inject the purified water of above-mentioned 3 taste medicine weight 8-12 times, open vacuum automatic switch, it is decompressed to negative pressure 0.5-1.5MP, and heat to 40 DEG C 50 DEG C, at 200MHz-300MHz ultrasonic 40-50 minute, medicinal liquid is leached, obtain first time filtrate, standby;
2) second time ultrasonic extraction: in ultrasonic extracting machine, inject the purified water of above-mentioned 3 taste medicine weight 6-16 times, open vacuum automatic switch, it is decompressed to negative pressure 0.5-1MP, and heat to 40 DEG C 50 DEG C, at 300MHz 500MHz ultrasonic 30-40 minute, medicinal liquid is leached, obtain second time filtrate, standby;
3) third time ultrasonic extraction: in ultrasonic extracting machine, injects the purified water of above-mentioned 3 taste medicine weight 4-6 times, opens vacuum automatic switch, it is decompressed to negative pressure 0.5-1MP, and heats to 40 DEG C 50 DEG C, at 400MHz 500MHz ultrasonic 1 hour, medicinal liquid is leached, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container stirring and evenly mixing;
5) step 4 gained medicinal liquid Chinese drug steaming device is concentrated, be concentrated into the 15% of filtrate volume, obtain concentrated solution, standby;
6) step 5 gained concentrated solution adding the ethanol of the content 95% of 3 times of weight, inserts in alcohol precipitation tank, 80 DEG C stand medicinal liquid and again filter after 80 hours, get rid of medicinal residues;
7) decompression recycling ethanol, filters with filter paper after reclaiming ethanol again, then carries out high temperature sterilize filtrate 30 minutes at 120 DEG C, obtain B component;
Four, component A and B component are mixed and stirred for uniformly, adjust pH value to 4.6~6.0 with sodium hydroxide, then carry out high temperature sterilize 30 minutes at 120 DEG C, the Chinese medicine composition of bovine mastitis must be treated.
The ultrasonic extracting machine used in the present invention is the efficient extracter of GL provided by Jining Tian Hua ultrasonic electronic Instrument Ltd., and described vacuum automatic switch is that the EN40-1AS Nene provided by Shanghai Chi Cheng automation equipment company limited can vacuum automatic switch.
It is bigger that decocting method extracts Moschus, Squama Manis and Venenum Bufonis herb liquid damage amount.The present invention use ultrasonic extraction technique extract Moschus, Squama Manis and Venenum Bufonis herb liquid.Ultrasound wave is a kind of high number mechanical wave, has the effect of cavitation, pulverizing, stirring in liquid medium, makes effective ingredient in medical material be dissolved in solvent by destroying the cell of vegetable drug, thus the purpose of herb liquid can be reached to extract.Chinese medicine ultrasonic extraction technological temperature low (usual 40-50 DEG C), safety is good, will not destroy the medicinal ingredient with thermally labile, facile hydrolysis or oxidation characteristic, extraction yield and efficiency high.
The Chinese medicine composition of the present invention is colourless or slightly yellow clear liquid, it is possible to oral or injection mode uses, it is preferable that the mode of injection such as intravenous injection, focal zone injection (namely nipple breast pond is injected) and intravenous drip.
Clinical trial shows, the Chinese medicine composition of the present invention has heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, carbuncle and ulcer speed are received, effect of active lactogenesis, various mastitis pathogenic bacteriums are had very strong killing action, can reconcile, improve the environment of breast gland cistern, destroying pathogen metabolic process, causing that pathogen metabolism is obstructed, thus killing pathogen.This medicine can also improve body to the endotoxic tolerance of pathogen, and antagonism bacterial exotoxin, to body pessimal stimulation, reduces the damage of cell, alleviates toxemia synptom;Fibrosis mammary gland glandular cell had activation and activates its vigor, promoting inflammatory or fiberization product solution absorption, recover effect of its milking capacity.
Indication: all kinds of mastitis of the animals such as milch cow, sheep, pig are had good curative effect by this product, including:
1, acute mastitis: suffering from poultry fever more than 40 DEG C, breast portion breast district or enlargement suddenly of whole breast suddenly in several hours, touch breast very hard, flexibility decrease, Pi Wen increases and has burning sensation.Pain reaction weight.The lactogenic of swelling breast district stops, and only has a small amount of inflammatory yellow water sample exudate or a small amount of curdling block to discharge during milking.
2, hemorrhagic mastitis: suffer from carcass temperature rise or normal, poor appetite, part breast district or whole breast swelling, touch and suffer from poultry pain reaction sensitivity, the livid purple color of breast surface, lactogenic stops, and has blood sample milk in various degree or leaner blood and slime sample inflammatory exudate during milking, discharges sometimes with blood clot and red curdling block.
3, chronic mastitis: suffer from poultry and mostly be that body temperature is normal or slight fever.Touching breast and have lump big as hard sense in various degree or children's's head, lactation amount declines, and milk is thin or becomes slightly yellow, sometimes has curdling block or cellulose sample coagulum to discharge.Extruding breast suffers from district slight pain.
4, fibrous mastitis: this kind of mastitis are acute mastitiss, owing to Therapeutic Method is improper or invalid by the Drug therapy many days of unsatisfactory curative effect, mammary gland glandular cell is made to be in inflammatory edema wet face state, a large amount of Fibrin exudations for a long time, and it is gathered in glandular cell, cause glandular cell's degeneration.Make pathological changes glandular cell's fibrosis and lose milking capacity.Finally resulting in mammary gland atrophy, milking capacity is remarkably decreased, and forms inclined breast.
5, suppurative mastitis: these type of mastitis are caused by pyogenic baeceria infects.Suppurative mastitis initial infection is had suppression abscess to be formed by this medicine, makes effect that swelling is disappeared.To the established case of abscess, protection network can be formed around suppuration stove, stop the pathogen effect to external diffusion.Detailed description of the invention
Preparation embodiment
Preparation process:
One, Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae, Herba Andrographis, Squama Manis, Moschus, Venenum Bufonis 9 taste crude drug are cleaned, clean, dry, it is ground into coarse powder, weighs Radix Bupleuri 10kg, Herba Violae 50kg respectively, Herba Taraxaci 60kg, Flos Chrysanthemi Indici 50kg, Fructus Forsythiae 30kg, Herba Andrographis 50kg, Moschus 0.8kg, Venenum Bufonis 2kg, Squama Manis 40kg, standby;
Two, the preparation of component A:
1) adding in extraction pot by Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae and Herba Andrographis, inject 2050kg purified water, heating, to boiling, decocts 3 hours, is leached by medicinal liquid, obtains first time filtrate, standby;
2) second time herb liquid extracts: in extraction pot, injects 1640kg purified water, and heating, to boiling, decocts 2 hours, leached by medicinal liquid, obtains second time filtrate, standby;
3) third time herb liquid extracts: in extraction pot, injects 1025kg purified water, and heating, to boiling, front is boiled 1 hour, is leached by medicinal liquid, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container and be uniformly mixed, precipitation, filter, obtain filtrate, standby;
5) step 4 gained filtrate is concentrated with Chinese drug steaming device, be concentrated into 2/5ths of filtrate volume, obtain concentrated solution, standby;
6) step 5 gained concentrated solution is added the ethanol of the content 95% of 1 times of weight, carries out precipitate with ethanol 72 hours, then by separation of ethanol, obtain first time fluid extract;The ethanol of the content 95% that first time fluid extract is added 0.8 times of weight carries out precipitate with ethanol 48 hours, is then peeled off ethanol, obtains second time fluid extract;The ethanol of the content 95% that second time fluid extract is added 0.7 times of weight carries out precipitate with ethanol 24 hours, then again separation of ethanol, obtains the crude drug extractum prepared, i.e. component A;
Three, the preparation of B component:
1) Moschus, Venenum Bufonis and Squama Manis are added in ultrasonic extracting machine, inject 430kg purified water, open vacuum automatic switch, be decompressed to negative pressure 1MP, and heat to 40 DEG C, at 300MHz ultrasonic 50 minutes, medicinal liquid is leached, obtains first time filtrate, standby;
2) second time ultrasonic extraction: in ultrasonic extracting machine, injects 342kg purified water, opens vacuum automatic switch, is decompressed to negative pressure 0.8MP, and heats to 45 DEG C, at 400MHz ultrasonic 40 minutes, is leached by medicinal liquid, obtains second time filtrate, standby;
3) third time ultrasonic extraction: in ultrasonic extracting machine, injects 215kg purified water, opens vacuum automatic switch, is decompressed to negative pressure 0.6MP, and heats to 50 DEG C, at 500MHz ultrasonic 1 hour, is leached by medicinal liquid, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container stirring and evenly mixing;
5) step 4 gained medicinal liquid Chinese drug steaming device is concentrated, be concentrated into the 15% of filtrate volume, obtain concentrated solution, standby;
6) by step 5 gained concentrated solution, adding the ethanol of the content 95% of 3 times of weight, insert in alcohol precipitation tank, 80 DEG C stand medicinal liquid and again filter after 80 hours, get rid of medicinal residues;
7) decompression recycling ethanol, filters with filter paper after reclaiming ethanol again, then carries out high temperature sterilize filtrate 30 minutes at 120 DEG C, obtain B component;
Four, component A and B component are mixed and stirred for uniformly, adjust pH value to 6.0 with sodium hydroxide, then carry out high temperature sterilize 30 minutes at 120 DEG C, the Chinese medicine composition of the bovine mastitis that obtains medical treatment.
Effect example
With suffer from mastitis milch cow for object, the therapeutic effect of the above-mentioned Chinese medicine composition for treating bovine mastitis is carried out clinical trial.
1, experiment material
(1) Chinese medicine composition of the treatment bovine mastitis that above-mentioned preparation embodiment obtains;
(2) experimental animal:
120 milch cows suffering from mastitis are chosen from outskirts of a town township, Beian City new street dairy village, the typical disease of these milch cows is: breast district has substantially inflammation red, swollen, hot, pain to show, lethargy, inappetence, milk is rare clearly, jaundice with obvious floccule, and milk yield is significantly lower than healthy cow of the same age.
2, experimental technique
Above-mentioned 120 cow heads are randomly divided into 4 groups, and respectively small dose group 30, middle dosage group 30, heavy dose organize 30, matched group 30, respectively group medication and method such as following table:
Group Case load Access times (my god) Administrated method
Small dose group 30 1 times/day Intravenous drip, each 30ml
Middle dosage group 30 1 times/day Intravenous drip, each 60ml
Heavy dose of group 30 1 times/day Intravenous drip, each 120ml
Matched group 30 0 times/day --
Medication group continuous use 10 days, every day periodic monitor milch cow situation, disease and situation of change of giving milk, judge curative effect according to the change of clinical symptoms and milk.Cure, effective and invalid criterion as follows:
Curing: breast district is red, swollen, hot, pain disappears, milch cow spirit, appetite is normal, and milk perusal is normal, and milk yield is recovered to healthy cow level of the same age.
Effective: breast district is red, swollen, hot, pain substantially alleviates, and appetite is recovered to some extent, and milk floccule substantially disappears or significantly reduces, and milk yield is gone up.
Invalid: breast district is red, swollen, hot, pain nothing substantially alleviates, lethargy, and inappetence, milk is still rare clearly or turns to be yellow, and flocculus is still more apparent.
3, experimental result
Small dose group is after medication 10 days, and milch cow spirit, appetite are normal, and milk perusal is normal, and milk yield is recovered, and milch cow is all fully recovered.Middle dosage group is after medication 8 days, and milch cow spirit, appetite are normal, and hollow billet is shinny, lots of natural fleshing, and milk perusal is normal, and milk yield is recovered, and milch cow is all fully recovered.Heavy dose of group is after medication 6 days, and milch cow spirit, appetite are normal, and body condition is clearly better, lots of natural fleshing, and milk perusal is normal, and milk yield is recovered, and milch cow is all fully recovered.
10 days rear milk areas of the milch cow of matched group are red, swollen, hot, pain without significant change, lethargy, inappetence, milk is rare clearly, jaundice and flocculus still more apparent, without significantly changing before compared to 10 days.
4, conclusion
Bovine mastitis is had good therapeutic effect by the Chinese medicine composition of the treatment bovine mastitis of the present invention, the milch cow suffering from mastitis can be made to recover Milk Production, the sound development improving milk production of cow and quality, promotion dairy is had very positive effect.
Above the example of the present invention is described in detail, but described content has been only the preferred embodiments of the present invention, it is impossible to be considered the practical range for limiting the present invention.All equalizations made according to the present patent application scope change and improvement etc., all should belong to the patent covering scope of the present invention.

Claims (2)

1. the Chinese medicine composition treating bovine mastitis, it is characterised in that: said composition is made up of component A and B component, and wherein component A is prepared from by the crude drug of following weight portion:
Radix Bupleuri: 10 Herba Violaes: 50 Herba Taraxacis: 60
Flos Chrysanthemi Indici: 50 Fructus Forsythiaes: 30 Herba Andrographis: 50;
B component is prepared from by the crude drug of following weight portion:
Moschus: 0.8 Venenum Bufonis: 2 Squama Maniss: 40.
2. the preparation method of the Chinese medicine composition of the treatment bovine mastitis described in claim 1, comprises the following steps:
One, Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae, Herba Andrographis, Squama Manis, Moschus are cleaned with Venenum Bufonis, clean, dry, pulverize into coarse powder, weigh by weight, standby;
Two, the preparation of component A:
1) Radix Bupleuri, Herba Violae, Herba Taraxaci, Flos Chrysanthemi Indici, Fructus Forsythiae and Herba Andrographis being added in extraction pot, inject the purified water of above-mentioned 6 taste 8-12 times of weight of medicine, heating, to boiling, decocts 3 hours, is leached by medicinal liquid, obtains first time filtrate, standby;
2) second time herb liquid extracts: in extraction pot, injects the purified water of above-mentioned 6 taste 6-10 times of weight of medicine, and heating, to boiling, decocts 2 hours, leached by medicinal liquid, obtains second time filtrate, standby;
3) third time herb liquid extracts: in extraction pot, injects the purified water of above-mentioned 6 taste 4-6 times of weight of medicine, and heating, to boiling, decocts 1 hour, leached by medicinal liquid, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container and be uniformly mixed, precipitation, filter, obtain filtrate, standby;
5) step 4 gained filtrate is concentrated with Chinese drug steaming device, be concentrated into 2/5ths of filtrate volume, obtain concentrated solution, standby;
6) step 5 gained concentrated solution is added the ethanol of the content 95% of 1 times of weight, carries out precipitate with ethanol 72 hours, then by separation of ethanol, obtain first time fluid extract;The ethanol of the content 95% that first time fluid extract is added 0.8 times of weight carries out precipitate with ethanol 48 hours, is then peeled off ethanol, obtains second time fluid extract;The ethanol of the content 95% that second time fluid extract is added 0.7 times of weight carries out precipitate with ethanol 24 hours, then again separation of ethanol, obtains the crude drug extractum prepared, i.e. component A;
Three, the preparation of B component:
1) Moschus, Venenum Bufonis and Squama Manis are added in ultrasonic extracting machine, inject the purified water of above-mentioned 3 taste medicine weight 8-12 times, open vacuum automatic switch, it is decompressed to negative pressure 0.5-1.5MP, and heat to 40 DEG C 50 DEG C, at 200MHz-300MHz ultrasonic 40-50 minute, medicinal liquid is leached, obtain first time filtrate, standby;
2) second time ultrasonic extraction: in ultrasonic extracting machine, inject the purified water of above-mentioned 3 taste medicine weight 6-16 times, open vacuum automatic switch, it is decompressed to negative pressure 0.5-1MP, and heat to 40 DEG C 50 DEG C, at 300MHz 500MHz ultrasonic 30-40 minute, medicinal liquid is leached, obtain second time filtrate, standby;
3) third time ultrasonic extraction: in ultrasonic extracting machine, injects the purified water of above-mentioned 3 taste medicine weight 4-6 times, opens vacuum automatic switch, it is decompressed to negative pressure 0.5-1MP, and heats to 40 DEG C 50 DEG C, at 400MHz 500MHz ultrasonic 1 hour, medicinal liquid is leached, obtains third time filtrate, standby;
4) by first time standby to step 1, step 2, step 3, for the second time, third time filtrate be placed in container stirring and evenly mixing;
5) step 4 gained medicinal liquid Chinese drug steaming device is concentrated, be concentrated into the 15% of filtrate volume, obtain concentrated solution, standby;
6) step 5 gained concentrated solution adding the ethanol of the content 95% of 3 times of weight, inserts in alcohol precipitation tank, 80 DEG C stand medicinal liquid and again filter after 80 hours, get rid of medicinal residues;
7) decompression recycling ethanol, filters with filter paper after reclaiming ethanol again, then carries out high temperature sterilize filtrate 30 minutes at 120 DEG C, obtain B component;
Four, component A and B component are mixed and stirred for uniformly, adjust pH value to 4.6~6.0 with sodium hydroxide, then carry out high temperature sterilize 30 minutes at 120 DEG C, the Chinese medicine composition of bovine mastitis must be treated.
CN201310569091.6A 2013-11-15 2013-11-15 A kind of Chinese medicine composition treating bovine mastitis Expired - Fee Related CN103720930B (en)

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Publication number Priority date Publication date Assignee Title
CN103751429A (en) * 2013-11-15 2014-04-30 北安市飞龙动物药厂 Traditional Chinese medicine composition for treating bovine mastitis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1634361A (en) * 2004-11-18 2005-07-06 西北农林科技大学 Chinese traditional medicine powder medicine for treating milk cow mastitis
CN101361894A (en) * 2008-08-29 2009-02-11 蒋保珍 Medicine for treating mastitis
CN101972444A (en) * 2010-07-13 2011-02-16 泰一和浦(北京)中医药研究院有限公司 Traditional Chinese medicine composition for treating mastitis and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1634361A (en) * 2004-11-18 2005-07-06 西北农林科技大学 Chinese traditional medicine powder medicine for treating milk cow mastitis
CN101361894A (en) * 2008-08-29 2009-02-11 蒋保珍 Medicine for treating mastitis
CN101972444A (en) * 2010-07-13 2011-02-16 泰一和浦(北京)中医药研究院有限公司 Traditional Chinese medicine composition for treating mastitis and preparation method thereof

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