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CN103691318A - Micro-scale substance separating method and capillary column transverse eletrochromatography separating device - Google Patents

Micro-scale substance separating method and capillary column transverse eletrochromatography separating device Download PDF

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CN103691318A
CN103691318A CN 201410005331 CN201410005331A CN103691318A CN 103691318 A CN103691318 A CN 103691318A CN 201410005331 CN201410005331 CN 201410005331 CN 201410005331 A CN201410005331 A CN 201410005331A CN 103691318 A CN103691318 A CN 103691318A
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separating
column
substances
separated
capillary
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CN 201410005331
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Chinese (zh)
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CN103691318B (en )
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李东浩
杨翠
任众
朴吉寿
刘翠翠
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延边大学
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Abstract

The invention discloses a micro-scale substance separating method and a capillary column transverse eletrochromatography separating device. The separating method comprises the step of introducing a sample into a separating column of a separating device, wherein the separating column is composed of a capillary tube which is used as an external electrode and a conductive material which is used as an internal electrode and penetrates through the capillary tube along the axial direction; the internal electrode and the external electrode are coaxial, and are respectively connected with the two ends of a power supply; the separating column transversely applies electric fields upwards, and electrophoresis effect is utilized for transversely transferring to-be-separated substances, so that contact probability of the to-be-separated substances and a column surface is increased, and separation is realized according to difference of interaction force sizes of the to-be-separated substances and the column surface; an absorption analyzing process of the to-be-separated substances and the column surface is completed by switching direction of the electric fields, so that resolution among the to-be-separated substances is improved. According to the invention, an electrophoretic technique and a chromatographic technique are integrated to realize effective separation of different substances, operation of the separating method and manufacturing of the special-purpose device are simple; and moreover, online detection can be realized.

Description

微尺度物质的分离方法及毛细管柱横向电色谱分离装置 The method of separating substances and microscale capillary column chromatographic separation apparatus laterally

技术领域 FIELD

[0001] 本发明涉及一种微尺度物质的分离方法及其专用设备,具体是指将电泳技术与色谱技术融合于一体的一种微尺度物质的分离方法及毛细管柱横向电色谱分离装置。 [0001] The present invention relates to a method of separating micro-scale apparatus and special materials, specifically refers to separation methods and chromatographic techniques electrophoresis merged in a substance and microscale capillary column chromatographic separation apparatus laterally.

背景技术 Background technique

[0002]目前,微尺度物质如细胞、生物大分子、合成颗粒物、胶体等,其分离和筛选已经成为当代各研究领域中一个优选的主要研究内容。 [0002] Currently, microscale materials such as cells, biological macromolecules, synthetic particles, colloids, etc., which has been isolated and screened in a field of study in Contemporary preferred main contents. 传统的分离技术很难同时分离尺寸范围较宽的样本,而且很难获得单一尺寸的目标物质,这些都将给后续研究带来不便。 Conventional separation techniques difficult to simultaneously separate a wide range of sample sizes, and the size is difficult to obtain a single target substance, which will follow-up studies have inconvenience. 为实现微尺度物质的分离,一些无阻塞、非填充型分离系统被建立,而绝大多数都属于连续型分离技术,此类技术有诸多优势,比如可以实现样品的连续分离与筛选、可实现大量样品的快速分离等。 To achieve microscale separation of substances, some of the non-blocking, non-filled separation system is set up, while the vast majority belong to the continuous separation technology, such technology has many advantages, such as continuous separation can be achieved with screening samples can be achieved rapid separation of large numbers of samples. 然而此类技术的装置结构和制作过程均比较复杂,并且设备制作精度要求高,对于多个组分的分离,装置制备更为困难。 However, the device structure and fabrication process of such techniques are relatively complex, and requires a high precision production equipment, more difficult to separate, for preparing a plurality of component devices. 多出口设计特点很难实现样品的在线检测,故此不适用于未知或复杂样品的分离分析。 Multi-outlet design features difficult to achieve online testing samples, therefore not suitable for separation and analysis of complex samples or unknown. 另外,大部分微分离技术与现有色谱技术相比,其分离度均较低。 Further, most of the micro-separation technique as compared with the prior art chromatography, which were lower resolution. 因此,连续型分离技术实际应用过程中必然面临一些挑战。 Therefore, continuous separation technology actual application process is bound to face some challenges. 基于以上局限,为更好的研究微尺度物质,需要开发一种高分辨率、装置制作和实验操作均较简单的技术。 Based on the above limitations, in order to better study the Microscale, we need to develop a high-resolution, device fabrication and experimental operation compared with simple techniques. 更为重要的是,要求技术能够容易实现样品的在线检测。 More importantly, the technology required to easily implement online testing samples.

发明内容 SUMMARY

[0003] 本发明的目的是为了克服非填充型分离系统中微尺度物质分离度低的不足,而提供一种微尺度物质的分离方法及其专用设备毛细管柱横向电色谱分离装置,本发明在分离装置的分离柱横向上引入电场,且柱表面引入色谱作用,微尺度物质进入分离柱后能够在电场作用下发生横向电迁移,通过物质的电迁移行为增加粒子与柱表面的接触几率,进而提高色谱作用的贡献,最终达到提高分离度的目的 [0003] The object of the present invention is to overcome the low degree of separation is less than the non-substance filled in micro-scale separation systems, to provide a method of separating material and special equipment microscale capillary lateral chromatographic separation apparatus according to the present invention introduced into the separation column separation apparatus the transverse electric field, and incorporated by column chromatography on a surface effect, lateral electromigration microscale can occur in the electric field into the separation column, to increase the probability of the contact with the cylindrical surface of the particles by electromigration behavior of matter, and thus improve chromatography and contribution, and ultimately to improve the degree of separation

为解决上述技术问题,本发明的技术方案如下: To solve the above technical problem, the technical solution of the present invention is as follows:

一种微尺度物质的分离方法,包括下列步骤: An isolated substance microscale method, comprising the steps of:

①样品由微量注射器引入进样器内; ① micro sample into a syringe sampler;

②打开毛细管柱横向电色谱分离装置的电源开关,使毛细管柱横向电色谱分离装置的分离柱横向上形成电场; ② lateral power switch open capillary column chromatographic separation apparatus, the capillary columns of laterally separated by column chromatography transverse electric field is formed on the apparatus;

③样品在由运载液的带动下由进样阀进入所述的分离柱,在外加横向电场作用下,样品中待分离物质在分离柱内发生横向迁移,待分离物质迁移到柱表面时,待分离物质会与柱表面发生相互作用; ③ When the sample entering the separation column by the injection valve driven by the carrier liquid, under the action of an applied transverse electric field, the sample substances to be separated migrate laterally in the separation column, the migration of substances to be separated cylindrical surface, to be the isolated material will interact with the cylindrical surface;

④电源正负极方向的切换,使待分离物质在分离柱内部横向上来回迁移,并与分离柱内表面发生吸附解吸过程,依据横向力的差异导致物质间分配系数存在差异,从而导致待分离物质沿流动方向有不同的速度,实现不同物质的分离; ④ positive and negative power switching direction of the substances to be separated migrate back and forth on the interior lateral separation column, and the adsorption and desorption processes with the inner surface of the separation column, according to the difference results in a lateral force coefficient distribution differences between species, leading to be separated species have different speeds in the flow direction, separation of different substances;

⑤分离物质以不同洗脱时间进入检测器内,进行在线检测。 ⑤ separating different substances into the elution time detector, online testing.

[0004] 所述的电场提供方式为脉冲电源或交流电源。 The electric field of the [0004] embodiment provides a pulsed or AC. [0005] 一种毛细管柱横向电色谱分离装置,包括有提供运载液的泵、进样器、检测器、电源和一端连接进样器,另一端连接检测器的分离柱,其中所述的分离柱包括有: [0005] The chromatographic separation apparatus for lateral capillary column, comprising providing a carrier liquid pump, injector, a detector, and a power supply connected to one end of the injector, separation column and the other end connected to the detector, wherein said isolated column include:

一个毛细管,该毛细管作为外电极; A capillary tube as an external electrode;

一根导电材料,该导电材料作为内电极,沿轴向贯穿毛细管并处于毛细管中心处,即内电极与与外电极同轴,内电极与与外电极连接电源两端。 An electrically conductive material, the conductive material as the internal electrodes, in the axial direction through the capillary and the capillary center, i.e., the inner electrode and the outer electrode coaxial with the inner electrode and the outer electrode is connected across the power supply.

[0006] 所述的导电材料为钼丝、金丝或银丝。 [0006] The conductive material is molybdenum wire, gold or silver.

[0007] 本发明的分离机理:待分离物质经进样阀引入分离柱后,在分离柱内,待分离物质将受横向电场作用发生横向移动即电泳技术,当待分离物质迁移到柱表面时,待分离物质与柱表面将发生相互作用即色谱技术。 [0007] The separation mechanism of the present invention: the substances to be separated after the introduction of the separation column via the injection valve in the separation column, substances to be separated will be subject to lateral movement i.e. electrophoresis transverse electric field takes place, when the substances to be separated migrate to the cylindrical surface , the substances to be separated cylindrical surface i.e. the interaction chromatographic techniques. 本发明中,待分离物质因自身性质不同,其所受横向作用力将产生差异,进而使待分离物质横向迁移距离产生差异,最终导致待分离物质与柱表面作用力存在差异,即待分离物质在本发明的分离柱内的分配系数存在差异,最终实现待分离物质的分离与分析。 In the present invention, substances to be separated due to their different nature, which will have suffered lateral force difference, thereby enabling the substances to be separated from the lateral migration of a difference, resulting in differences in the surface of the substance to be separated by column force, i.e. substances to be separated there is a difference in the distribution coefficients of the separation column of the present invention, the separation and analysis of substances to be separated eventually. 在本发明系统内,利用电场方向的切换,使待分离物质在分离柱横向上有反复迁移过程,进而使待分离物质与柱表面之间有吸附解吸过程,通过改变电场的频率,提高理论塔板数,进而提高无阻塞、非填充型分离系统的分离度。 Within the system of the present invention, the electric field direction by the switching of the substance to be separated in the migration process is repeated on the lateral separation column, thereby enabling adsorption desorption substances to be separated between the cylindrical surface, by changing the frequency of the electric field, improving the theoretical column number plate, blocking further increase resolution, non-filled separation system.

[0008] 上述的自身性质包括尺寸大小或分子量、表面电荷以及扩散系数;上述的横向作用力为电泳力和黏滞阻力;上述的待分离物质与柱表面之间的相互作用可以为离子相互作用(静电作用)、非极性相互作用或离子相互作用和非极性相互作用同时存在。 [0008] The properties include its own size or molecular weight, surface charge and a diffusion coefficient; and the lateral force and the electrophoretic force to viscous drag; the above-described interaction between the substance to be separated and the cylindrical surface can be an ionic interaction (electrostatic interaction), or non-polar interactions and ionic interactions exist non-polar interactions.

[0009] 总之,本发明通过将分离柱与检测器的在线联用,克服了当前微分离技术对未知的或复杂的微尺度物质难以在线分离分析的困难。 [0009] In summary, the present invention is by separating column and the detector line coupling, overcomes the difficulties of this microdissection technique unknown or difficult to separate the complex substance microscale online analysis. 另外,本发明有效的将电泳技术和色谱技术融于一体,大大提高了无阻塞、非填充型分离柱的理论塔板数,进而实现样品的高效分离,有望获得单一尺寸的微尺度物质,为微尺度物质的后续研究奠定基础。 Further, the present invention is effective to electrophoresis and chromatographic techniques into one, greatly improving the number of theoretical plates blocking, non-filled column separation, thus achieving efficient separation of the sample, is expected to receive a single size microscale material as microscale follow-up study of the foundation.

附图说明 BRIEF DESCRIPTION

[0010] 图1为本发明毛细管柱横向电色谱分离装置示意图。 [0010] Fig 1 a schematic view of a capillary column chromatographic separation apparatus of the present invention the lateral.

[0011] 图2为本发明实施例中不同尺寸聚苯乙烯颗粒分离效果图。 [0011] Example 2 polystyrene particles of different size separation embodiment of the invention FIG.

[0012] 图3为本发明实施例中不同表面电荷聚苯乙烯颗粒分离效果图。 [0012] Figure 3 a different embodiment the surface charge of the particle separation polystyrene embodiment of the present invention FIG.

具体实施方式: detailed description:

在图1中,1、运载液,2、六通进样阀,3、样品,4、废液,5、PEEK管,6、导电材料一内电极, In Figure 1, 1, carrier liquid, two, six-way injection valve, 3, sample 4, the waste liquid, 5, PEEK tube 6, an inner electrode of conductive material,

7、毛细管一外电极,8、电源,9、检测器,10、三通阀。 7, a capillary outer electrode 8, the power supply 9, a detector 10, a three-way valve.

[0013] 参考图1,一种毛细管柱横向电色谱分离装置,包括有提供运载液的泵、进样器、检测器、电源和一端连接进样器,另一端连接检测器的分离柱,其中所述的分离柱包括有:一个毛细管7,该毛细管7作为外电极;一根导电材料6,所述的导电材料6可为钼丝、金丝或银丝,该导电材料6作为内电极,沿轴向贯穿毛细管7,为保证分离柱内各处待分离物质所受横向电场力相同,内电极必须置于外电极中心处,即内电极与与外电极同轴,并分别连接电源8两端,进而在分离柱横向上产生电场。 [0013] Referring to FIG 1 a lateral capillary column chromatographic separation apparatus, comprising providing a carrier liquid pump, injector, a detector, and a power supply connected to one end of the injector, separation column and the other end connected to the detector, wherein said separation column comprising: a capillary tube 7, the capillary tube as an external electrode 7; 6 an electrically conductive material, said conductive material 6 may be a molybdenum wire, gold or silver, the electrodes as the conductive material 6, 7 axially through the capillary, in order to ensure the entire substance to be separated within the separation column suffered the same transverse electric field force, the outer electrodes must be placed at the center of the electrode, i.e., the inner electrode and the outer electrode coaxially, and two are connected to power supply 8 end, thereby generating a transverse electric field across the separation column. 本发明无需使用常规液相系统的高压泵,进样器采用六通进样阀2,为将待分离物质引入分离柱,并实现在线检测,分离柱的毛细管7 —端通过固定设置的三通阀10连接六通进样阀2以便引入样品3,另一端通过固定设置的三通阀10连接检测器9以便实分离物质的在线检测,处于毛细管外侧的导电材料6两端由PEEK管5承接。 The present invention does not require a high pressure pump of a conventional liquid system, with six injector through the injection valve 2, is introduced into the separation column material to be separated, and line measurement, a capillary separation column 7-- fixed end of the three-way 6 both ends of six-valve 10 is connected to the injection valve 2 into the sample 3, and the other end is fixed by three-way valve 10 is connected to the detector 9 to detect line separating solid material, a conductive material is located outside of the capillary tube receiving from PEEK 5 . [0014] 利用上述专用的毛细管柱横向电色谱分离装置而公开的一种微尺度物质的分离方法,包括下列步骤: [0014] The use of the dedicated lateral capillary column chromatographic separation apparatus disclosed microscale method for separating substance, comprising the steps of:

①样品3由微量注射器引入六通进样阀2内; ① Sample 3 is introduced by a microsyringe six-way injection valve 2;

②打开电源8的开关,使分离柱横向上形成电场; ② switch on the power supply 8 so that the transverse electric field is formed on the separation column;

③样品3在由泵提供的运载液I的带动下由六通进样阀2、三通阀10进入分离柱,在外加横向电场作用下,样品中待分离物质在分离柱内发生横向迁移; ③ sample 3 sample into a six-driven carrier liquid I is provided by the pump valve 2, the three-way valve 10 into the separation column, under the action of an applied transverse electric field, the sample substances to be separated in the lateral migration of the separation column;

④电源8的正负极方向切换,使待分离物质在横向上来回迁移,并与柱表面发生吸附解吸过程,横向力的差异使物质间分配系数存在差异,进而导致待分离物质沿流动方向有不同的速度,实现不同物质的分离; ④ direction of the positive and negative power supply switch 8, so that substances to be separated migrate back and forth in the transverse direction, and the adsorption and desorption processes occurring cylindrical surface, the lateral force difference in the distribution coefficient differences between the species, leading to substances to be separated in the flow direction different speeds, to achieve separation of different species;

⑤分离物质以不同洗脱时间由三通阀10进入检测器9内,进行在线检测,同时排出废液4。 ⑤ different elution time separating substances into the detector 9 by a three-way valve 10, line detection, while discharging waste 4.

[0015] 在具体分离操作过程中,所述的电源为脉冲电源或交流电源。 [0015] In particular during the separating operation, the pulsed power supply or AC power supply. 所述的横向作用力为电泳力、黏滞阻力、待分离物质与柱表面之间的相互作用力;所述的待分离物质与柱表面之间的相互作用可以为离子相互作用(静电作用)、非极性相互作用或离子相互作用与非极性相互作用同时存在。 The lateral force is electrophoretic force, viscous drag, until the separation force between the material and the surface of the column; be separated according to the interaction between the substance can interact with the surface of the column (electrostatic interaction) ion , non-polar interactions or ionic interactions with non-polar interactions exist.

[0016] 在毛细管柱横向电色谱系统中,当分离柱固定时,待分离物质与柱表面之间的吸附解析次数直接影响待分离物质间的分离度。 [0016] In the transverse electric capillary column chromatographic system, when the separation column is fixed, the number of adsorption analytical substances to be separated between the cylindrical surface directly affect the degree of separation between the substances to be separated. 在毛细管柱横向电色谱技术中,通过优化电场大小(电压和频率)、电泳时间、运载液流速等参数可提高待分离物质间的分离度。 In the transverse electric capillary column chromatography, by optimizing the size of the electric field (voltage and frequency), electrophoresis time, the carrier liquid flow rate and other parameters can increase the degree of separation between the substances to be separated. 优化结果显示,外加电场频率/和持续时间ί与洗脱时间(6成正相关,电压U对无影响,但影响颗粒的保留量和峰形;另外,电压U和电场持续时间t与半峰宽#1/2成负相关,f与#1/2成正相关;一定范围内,运载液流速影响不大。本发明中在电场作用下,待分离物质将发生横向迁移,因待分离物质横向迁移距离不同使其与柱表面的相互作用力存在差异,即分配系数不同。另外,本发明中采用脉冲频率的变化使待分离物质与柱表面发生吸附解吸过程。基于以上两个原因,最终实现微尺度的不同物质之间的分离。 Optimization results show that the applied electric field positively related to the frequency / and duration ί elution time (60%, voltage U no effect on, but the impact of particle retention and peak shape; Further, the voltage U and the electric field duration t and the half width # 1/2 negative correlation, f positively correlated with # 1/2 percent; within a certain range, not the flow rate of the influence of the carrier liquid according to the present invention, in the electric field, substances to be separated transverse migration will occur, due to substances to be separated transverse migration. interaction forces differ from the cylindrical surface so different, i.e., different distribution coefficient. Further, the present invention changes the frequency of the pulse so that the substances to be separated cylindrical surface adsorption-desorption process takes place. For these two reasons, ultimately micro separation between the different substances scales.

[0017] 本发明在实际分离过程中对微尺度物质实现分离,以聚苯乙烯颗粒物为例评价其分辨率。 [0017] The present invention achieves the separation of the Microscale actual separation process, the polystyrene particles Example evaluated resolution. 毛细管柱横向电色谱技术分尚条件如下,分尚柱长度为20 cm;电压为1.2 V ;频率为0.5 Hz ;电场施加时间为10 min ;脉冲周期分别为0.4和0.6 ;运载液为蒸馏水;流速为1.0mL/h。 Capillary transverse electric chromatography points still under the following conditions, sub still column length is 20 cm; voltage of 1.2 V; frequency of 0.5 Hz; electric field application time 10 min; pulse period were 0.4 and 0.6; carrier liquid is distilled water; flow rate It was 1.0mL / h. 如图2、图3。 2, FIG. 从图2、3可以得出,毛细管柱横向电色谱分离技术实现了不同微尺度物质(不同尺寸或不同表面电荷)分离的目的。 It can be derived from FIG. 3, the lateral capillary column chromatographic separation techniques to achieve the object of the Microscale different (different sizes or surface charge) isolated. 本发明公开了毛细管柱横向电色谱技术,首次有效的将电泳技术和色谱技术融于一体,提高待分离物质间的分辨率,获得单一尺寸的微尺度物质,而且该技术可以实现在线检测。 The present invention discloses a transverse electric capillary column chromatography, first time effective to electrophoresis and chromatographic techniques into one, to improve the resolution between the substances to be separated, to obtain a single substance microscale dimension, and the line detection techniques may be implemented.

Claims (4)

  1. 1.一种微尺度物质的分离方法,包括下列步骤: ①样品由微量注射器引入进样器内; ②打开毛细管柱横向电色谱分离装置的电源开关,使毛细管柱横向电色谱分离装置的分离柱横向上形成电场; ③样品在由运载液的带动下由进样阀进入所述的分离柱,在外加横向电场作用下,样品中待分离物质在分离柱内发生横向迁移,待分离物质迁移到柱表面时,待分离物质会与柱表面发生相互作用; ④电源正负极方向的切换,使待分离物质在分离柱内部横向上来回迁移,并与分离柱内表面发生吸附解吸过程,依据横向力的差异导致物质间分配系数存在差异,从而导致待分离物质沿流动方向有不同的速度,实现不同物质的分离; ⑤分离物质以不同洗脱时间进入检测器内,进行在线检测。 1. A method of separating microscale substance, comprising the following: ① From the sample into a micro-syringe injector; ② transverse electric power switch capillary column chromatographic separation apparatus, the capillary separation column by column chromatography transverse electric apparatus the transverse electric field is formed; ③ driven by the carrier sample at liquid entering the separation column by the injection valve, under the action of an applied transverse electric field, the sample substances to be separated migrate laterally in the separation column, the migration to the substances to be separated when the cylindrical surface, the substances to be separated interact cylindrical surface; ④ positive and negative power switching direction of the substances to be separated migrate back and forth on the interior lateral separation column, and the adsorption and desorption processes with the inner surface of the separation column, according to the transverse the difference in force leads to differences among partition coefficients substance, causing the substance to be separated have different speeds in the flow direction, separation of different substances; ⑤ separation of substances with different elution times into the detector line detection.
  2. 2.根据权利要求1所述的一种微尺度物质的分离方法,其特征在于所述的电场提供方式为脉冲电源或交流电源。 2. A method for separating the microscale substance according to claim 1, wherein said field mode to provide pulse or AC.
  3. 3.一种毛细管柱横向电色谱分离装置,包括有提供运载液的泵、进样器、检测器、电源和一端连接进样器,另一端连接检测器的分离柱,其特征在于所述的分离柱包括有: 一个毛细管,该毛细管作为外电极; 一根导电材料,该导电材料作为内电极,沿轴向贯穿毛细管并处于毛细管中心处,即内电极与外电极同轴,内电极与外电极连接电源两端。 Lateral chromatographic separation apparatus A capillary column, comprising providing a carrier liquid pump, injector, a detector, and a power supply connected to one end of the injector, separation column and the other end connected to the detector, characterized in that said separation column comprising: a capillary tube as an external electrode; an electrically conductive material, the conductive material as the internal electrodes, in the axial direction through the capillary and the capillary center, i.e., a coaxial inner and outer electrodes, the inner electrode and the outer electrodes are connected across the power supply.
  4. 4.根据权利要求3所述的一种毛细管柱横向电色谱分离装置,其特征在于所述的导电材料为钼丝、金丝或银丝。 4. lateral chromatographic separation capillary column device according to claim 3, wherein said conductive material is a molybdenum wire, gold or silver.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104359995A (en) * 2014-12-04 2015-02-18 延边大学 Method for separating bio-macromolecules of flow type stationary phase in column by utilizing electromagnetic field
CN104383716A (en) * 2014-12-04 2015-03-04 延边大学 Method for modifying mesoporous material on surface of electrode in transverse electric field capillary column
CN105527335A (en) * 2016-02-02 2016-04-27 中国科学院电子学研究所 Photoionization detector

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1736538A (en) * 2005-08-02 2006-02-22 天津大学 Method for enhancing dynamic absorption volume of ion exchange absorbent of transverse electric field
CN2911691Y (en) * 2006-06-21 2007-06-13 戴朝政 Detention eliminating chromatograhic column
US8137512B2 (en) * 2006-09-04 2012-03-20 National Institute Of Advanced Industrial Science And Technology Process for analyzing sample by capillary electrophoresis method
CN102680610A (en) * 2011-03-18 2012-09-19 中国科学院大连化学物理研究所 Gas chromatographic column separated through electric filed control and applications thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1736538A (en) * 2005-08-02 2006-02-22 天津大学 Method for enhancing dynamic absorption volume of ion exchange absorbent of transverse electric field
CN2911691Y (en) * 2006-06-21 2007-06-13 戴朝政 Detention eliminating chromatograhic column
US8137512B2 (en) * 2006-09-04 2012-03-20 National Institute Of Advanced Industrial Science And Technology Process for analyzing sample by capillary electrophoresis method
CN102680610A (en) * 2011-03-18 2012-09-19 中国科学院大连化学物理研究所 Gas chromatographic column separated through electric filed control and applications thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
袁瑞娟等: "毛细管电色谱技术研究进展", 《化学进展》, vol. 18, no. 9, 30 September 2006 (2006-09-30) *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104359995A (en) * 2014-12-04 2015-02-18 延边大学 Method for separating bio-macromolecules of flow type stationary phase in column by utilizing electromagnetic field
CN104383716A (en) * 2014-12-04 2015-03-04 延边大学 Method for modifying mesoporous material on surface of electrode in transverse electric field capillary column
CN104359995B (en) * 2014-12-04 2015-12-30 延边大学 The method of biomacromolecule separation flow type stationary phase of the column using an electromagnetic field
CN105527335A (en) * 2016-02-02 2016-04-27 中国科学院电子学研究所 Photoionization detector

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