CN103636374A - Light supplement seedling culture method for Lijiang spruce container seedlings and application thereof - Google Patents
Light supplement seedling culture method for Lijiang spruce container seedlings and application thereof Download PDFInfo
- Publication number
- CN103636374A CN103636374A CN201310554487.3A CN201310554487A CN103636374A CN 103636374 A CN103636374 A CN 103636374A CN 201310554487 A CN201310554487 A CN 201310554487A CN 103636374 A CN103636374 A CN 103636374A
- Authority
- CN
- China
- Prior art keywords
- seedling
- light filling
- container
- spruce
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000218657 Picea Species 0.000 title claims abstract description 56
- 239000013589 supplement Substances 0.000 title description 3
- 238000012136 culture method Methods 0.000 title 1
- 230000012010 growth Effects 0.000 claims abstract description 50
- 238000000034 method Methods 0.000 claims abstract description 46
- 238000009331 sowing Methods 0.000 claims abstract description 13
- 230000000366 juvenile effect Effects 0.000 claims abstract description 10
- 239000002689 soil Substances 0.000 claims description 34
- 239000003415 peat Substances 0.000 claims description 17
- 210000003608 fece Anatomy 0.000 claims description 11
- 239000010871 livestock manure Substances 0.000 claims description 11
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 10
- 239000011701 zinc Substances 0.000 claims description 10
- 229910052725 zinc Inorganic materials 0.000 claims description 10
- 241000287828 Gallus gallus Species 0.000 claims description 9
- 239000002686 phosphate fertilizer Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 7
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 7
- 239000011790 ferrous sulphate Substances 0.000 claims description 7
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 7
- 239000011574 phosphorus Substances 0.000 claims description 7
- 229910052698 phosphorus Inorganic materials 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 6
- 239000005416 organic matter Substances 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 3
- 229910052692 Dysprosium Inorganic materials 0.000 claims description 3
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 3
- AEEAZFQPYUMBPY-UHFFFAOYSA-N [I].[W] Chemical compound [I].[W] AEEAZFQPYUMBPY-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- AEJIMXVJZFYIHN-UHFFFAOYSA-N copper;dihydrate Chemical compound O.O.[Cu] AEJIMXVJZFYIHN-UHFFFAOYSA-N 0.000 claims description 3
- KBQHZAAAGSGFKK-UHFFFAOYSA-N dysprosium atom Chemical compound [Dy] KBQHZAAAGSGFKK-UHFFFAOYSA-N 0.000 claims description 3
- 229910052734 helium Inorganic materials 0.000 claims description 3
- 239000001307 helium Substances 0.000 claims description 3
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 3
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 229910052754 neon Inorganic materials 0.000 claims description 3
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 239000012286 potassium permanganate Substances 0.000 claims description 2
- -1 and 5~10 parts Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 230000003595 spectral effect Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 2
- 206010021033 Hypomenorrhoea Diseases 0.000 abstract 3
- 230000009469 supplementation Effects 0.000 abstract 2
- 230000001502 supplementing effect Effects 0.000 abstract 2
- 230000001934 delay Effects 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 241000351396 Picea asperata Species 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 230000000050 nutritive effect Effects 0.000 description 4
- 244000061458 Solanum melongena Species 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 230000005059 dormancy Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 230000009605 growth rhythm Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000011449 brick Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000009746 freeze damage Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 229920006255 plastic film Polymers 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000009333 weeding Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241001669679 Eleotris Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 239000005794 Hymexazol Substances 0.000 description 1
- 241000968377 Picea likiangensis Species 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241001515806 Stictis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015709 bud dormancy process Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005429 filling process Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000011464 hollow brick Substances 0.000 description 1
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000027665 photoperiodism Effects 0.000 description 1
- 230000008638 plant developmental process Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Cultivation Of Plants (AREA)
Abstract
本发明涉及一种丽江云杉容器苗的补光育苗方法，以及该方法的应用。所述方法包括：播种丽江云杉种子后，对容器苗进行补光处理；补光时间包括：从容器苗的幼苗脱壳后开始补光，在容器苗生长季节连续补光两年；容器苗生长第一年从容器苗的幼苗脱壳后开始补光，补光时段为22:00～2:00，补光100～110天；容器苗生长第二年从容器苗展叶时开始补光，补光时段为21:00～1:00，补光110～120天。本发明首要解决了丽江云杉育苗过程中的苗期和幼龄期生长缓慢的问题，大大加速了丽江云杉苗期生长，延迟封顶，使苗木提前出圃；可有效保证丽江云杉的成活率和苗圃保存率，为大规模供应丽江云杉造林提供一种周期短且成本低廉的方法。The invention relates to a method for growing seedlings of Lijiang spruce container seedlings with supplementary light and the application of the method. The method comprises: after sowing the spruce spruce seeds of Lijiang, carrying out supplementary light treatment on the container seedlings; the supplementary light time includes: supplementing the light after the seedlings of the container seedlings are shelled, and supplementing the light continuously for two years during the growing season of the container seedlings; In the first year of growth, light supplementation starts after the seedlings of the container seedlings have shelled, and the supplementary light period is 22:00-2:00, and the supplementary light is 100-110 days; in the second year of growth of the container seedlings, the light supplementation starts when the container seedlings unfold their leaves , the supplementary light period is from 21:00 to 1:00, and the supplementary light period is 110 to 120 days. The present invention primarily solves the problem of slow growth at the seedling stage and juvenile stage of Lijiang spruce seedlings, greatly accelerates the growth of Lijiang spruce seedlings, delays capping, and enables seedlings to emerge from the nursery in advance; it can effectively ensure the survival rate of Lijiang spruce and nursery preservation rate, providing a short-cycle and low-cost method for large-scale supply of Lijiang spruce afforestation.
Description
Technical field
The present invention relates to the seedling-cultivating method of a Plants, particularly, relate to the light filling seedling-cultivating method of a kind of lijiang spruce container seedling, and the application of the method.
Background technology
Lijiang spruce (Picea likiangensis) Pinaceae, Picea.Originate in the Sichuan Province China west and south, northwestern Yunnan Province alpine belt, form the simple woods of large area.Timber is light and soft, durable, can be for materials such as building, sleeper, utensil, papermaking.The grain of wood is straight, compact structure, and material is good, easily processing.The cold warm nature coniferous species that Ta Shi China is important, has the advantages that growth is fast, material is good, is the Major Tree Species Planted of its area, is also used as flower garden ornamental tree species and generally cultivates.Yet lijiang spruce seedling stage and juvenile stage poor growth, growing-seedling period reaches 4～6 years.Growing-seedling period is long, cost is high, economic benefit is low, and supply falls short of demand to cause lijiang spruce breeding seedling, can not meet afforestation Production requirement far away.Therefore,, on the seedling growing process of lijiang spruce, need to develop a good approach.
In plant growth and development process, the photoperiod is a very important influence condition, and the whole life process of plant all completes under photoperiodic impact.The characteristic of woody plant anniversary growth has determined to be difficult to be divided into long day plant or short day plant on stricti jurise, and still, in the growth of woody plant anniversary, the photoperiod still has important impact for its growth rhythm.Photoperiod to height growth stop, hibernaculum formation, leaves, dormancy induction etc. play important control action.The same with the photoperiodism known to general, the photoperiodic reaction of trees is decided by the length at night, i.e. number during every day dark.As far back as nineteen twenty-three Garner and Allard, just proved that the photoperiod affects growth and the dormancy of woody plant, to be adjusting activity growth formed and changed and cold resistance is tempered the important factor changing to dormancy to terminal bud the photoperiod.It is prerequisite that apical growth stops, and terminal bud dormancy subsequently forms, and then height of formation cold resistance.
Open country is grown seedlings and is caused that for preventing that autumn end from not binding freeze injury need to shorten the photoperiod, and natural lighting time span is different along with the difference of latitude.In the season of growth, the northern provenance of dragon spruce is than the sunshine of southern provenance experience longer time, result causes northern Picea asperata seedlings under the long-day, to be difficult to form terminal bud, so best method is exactly artificial shortening photoperiod and shading treatment, impels northern Picea asperata seedlings growth to stop and forming terminal bud.In good time short-day is processed the freezing resistance that can increase Picea asperata seedlings, also can improve dragon spruce degree of lignification simultaneously.Otherwise extended photoperiod can be postponed nursery stock and be bound, promote seedling to continue growth, reach the object of quick seedling.
Container nursery is applied to those dragon spruce seedlings that derives from north latitude high latitude or high altitude localities especially, and wherein extended photoperiod is the key of accelerating seedling growth.Can adopt multiple light filling mode to extend the photoperiod in container seedling seedling raising process, thereby maintain height of seedling, grow to reach expection height, make nursery stock go out in advance garden.
Both at home and abroad the dragon spruce of some classifications has been adopted to the measure of growing seedlings of container nursery light filling, and obtained certain effect, but also there is some problems: concrete light filling time, light filling mode etc. also do not form the system of improving, and the effect that different classes of dragon spruce kind produces light filling is inconsistent, and dragon spruce interspecific difference is remarkable.
So far, bibliographical information does not carry out the light filling research of aspect of growing seedlings to lijiang spruce, if can develop relevant technology, will certainly, cultivating aspect lijiang spruce, have very wide prospect and huge economic worth.
Summary of the invention
In order to solve the problems of the technologies described above, one of object of the present invention is, a kind of light filling seedling-cultivating method that is suitable for specially the container seedling of lijiang spruce is provided, and the method has primarily solved seedling stage and the poky problem of juvenile stage in lijiang spruce seedling raising process.
Another object of the present invention is to, the application of described method in cultivating lijiang spruce container seedling is provided.
In order to realize foregoing invention object, the light filling seedling-cultivating method of lijiang spruce container seedling provided by the invention, comprising: after sowing lijiang spruce seed, container seedling is carried out to light filling processing;
The light filling time comprises: from the seedling shelling of container seedling, start light filling, container seedling growth continuous light filling in season 2 years;
Container seedling growth First Year starts light filling from the seedling shelling of container seedling, and the light filling period is 22:00～2:00, light filling 100～110 days, preferably 100 days;
Container seedling growth Second Year starts light filling when container seedling is opened up leaf, and the light filling period is 21:00～1:00, light filling 110～120 days, preferably 120 days.
First carry out prior to seeding the selection of container, select the container and the disposing way that are beneficial to lijiang spruce growth.
Further, from container seedling approximately 80% seedling shelling, start light filling.
Wherein, the light source that described light filling is processed is: incandescent lamp, high-pressure mercury lamp, fluorescent lamp, sunlight dysprosium lamp, iodine-tungsten lamp, neon lamp or helium lamp, preferably fluorescent lamp.
When the light source of described light filling processing is fluorescent lamp, the intensity of illumination that light filling is processed is 1～6 μ molm
-2s
-1.
Further, described fluorescent light spectrum wavelength scope is 400～700nm.
Method of the present invention, cultivates lijiang spruce container seedling seedling medium used, comprising: the rural area soil that volume ratio is 3:2 and peat soil, wherein add chicken manure, phosphate fertilizer, zinc to mix p and s acid ferrous iron again.
Wherein, in the rural area soil of per unit volume and peat soil (both total amounts), the parts by weight of chicken manure are 10～20 parts, and the parts by weight of phosphate fertilizer are 5～10 parts.
Wherein, in the rural area soil of per unit volume and peat soil (both total amounts), the parts by weight that zinc is mixed phosphorus are 0.2～0.8 part, preferably 0.5 part; The parts by weight of ferrous sulfate are 1.2～1.8 parts, preferably 1.5 parts.
In the present invention, the unit of volume is cubic meter, and the unit of area is square metre, and parts by weight are kilogram (or kilogram), can be depending on the concrete condition of production.
Wherein, in described seedling medium, the content of organic matter is 8.0～8.5g/kg, preferably 8.2g/kg; The pH value of seedling medium is 6.2～6.8, preferably 6.5; Slant acidity.
Wherein, within 1 day, use prior to seeding 0.3%(mass fraction) liquor potassic permanganate to described seedling medium disinfection.
Method of the present invention, lijiang spruce seed prior to seeding, also comprises: with 45 ℃ of water presoaking and germinatings 24 hours, after wet concentration seed, remain on 25 ℃; Use afterwards 0.3%(mass fraction) disinfecting solution of potassium permanganate 30 minutes, and rinse well with clear water, then in seed, admix the sawdust of 1 times of volume of seed, carry out vernalization; When seed has 30～40% to show money or valuables one carries unintentionally, start sowing.
Method of the present invention, also comprises the management of the container seedling of lijiang spruce being carried out to juvenile stage and seedling stage, comprising: topdress and diseases prevention etc.
Wherein, described fertilizing type comprises: after planting First Year after seedling height growth starts every 20 days, press in the seedbed of per unit area, use the nitrogenous fertilizer solution that 2 parts of weight percentages are 0.3 ‰ (being foliage fertilizer) once, altogether use 6 times (using 100 days); Then equally every 20 days, in the seedbed of per unit area, use potassium dihydrogen phosphate that 2 parts of weight percentages are 0.5 ‰ once, altogether use twice, promote nursery stock lignification.All use 2 parts at every turn.
After planting Second Year, every 15 days after nursery stock exhibition leaf, in the seedbed of per unit area, is used respectively nitrogenous fertilizer solution that 3 parts of percetages by weight are 0.3 ‰ once, altogether uses 9 times (using 120 days); Then equally every 15 days, in the seedbed of per unit area, use potassium dihydrogen phosphate that 3 parts of percetages by weight are 0.5 ‰ once, altogether use twice, promote nursery stock lignification.All use 3 parts at every turn.
Wherein, described diseases prevention mode comprises: after nursery stock is unearthed, every 7 days, to blade face and stromal surface, spray carbendazim, Kocide SD or a hymexazo (hymexazol) solution, three kinds of solution need be used by turns.
Method of the present invention, implements for spring sowing.
The present invention also provides the application of above-mentioned any one method in cultivating lijiang spruce container seedling.
Wherein, lijiang spruce seed comes from Shangri-La, Yunnan Province county.
Method of the present invention, water and fertilizer management is better, and the afforestation of going up a hill time can, together with container, effectively guarantee plant percent.
The invention provides a kind of light filling seedling-cultivating method that is suitable for specially the container seedling of lijiang spruce, being about to specific condition (as light filling mode and light filling time etc.) is applied in specific dragon spruce kind, form perfect system, primarily solved seedling stage and the poky problem of juvenile stage in lijiang spruce seedling raising process.Secondly, the present invention has accelerated lijiang spruce seedling growth greatly by light filling, postpones to bind, and makes nursery stock go out in advance garden; Can effectively guarantee survival rate and the nursery storage rate of lijiang spruce, and neat, the seedling strain of emerging grows complete stalwartness, for extensive supply lijiang spruce afforestation provides a kind of method short and with low cost of cycle.
Accompanying drawing explanation
Fig. 1 is that light filling fluorescent lamp spectrum radiant illumination used distributes.
Embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
The present invention's material used is can purchase available conventional material (or product) from the market, and NM operational means is also ordinary skill in the art means.
Lijiang spruce seed of the present invention comes from Shangri-La, Yunnan Province county.
General lijiang spruce seed is all applicable to the present invention.
The present invention's fluorescent lamp used is the emerging standing grain board fluorescent lamp that Hebei University of Science and Technology produces, power 36W, and photochromic aubergine, wavelength main peak 450nm and 640nm, spectral analysis figure is as shown in Figure 1.
The present invention measures intensity of illumination and adopts: hand-held import illuminometer.
In the present invention, container seedling growth First Year and second refers to start the year (12 months and 24 months) of calculating from the seedling shelling of container seedling every year.
When the present invention specifically implements, in early April, 2008, start sowing, on May 8th, 2008 (80% seedling of container seedling starts shelling), start the light filling of container seedling growth First Year, can stop down on August 16th, 2008; On April 17th, 2009 (during container seedling exhibition leaf), start the light filling of container seedling growth Second Year, can stop down on August 15th, 2009.
The present invention sows experiment at Xiaolong mountain in Gansu, and the more past south latitude area light filling time can extend again, and can suitably adjust according to growing state the exact date that light filling starts and finishes.
On May 20th, 2008 (seedling starts high growth), start after planting topdressing of First Year, on April 20th, 2009 (nursery stock starts to open up leaf), start after planting topdressing of Second Year, in 2010, start outplanting.
Lijiang spruce open country is grown seedlings growth generally only in early and middle ten days growth in mid-April to June, and late June starts to bind; And breeding and seedling nursing with equipment is under light filling condition, preference temperature, particularly improves the temperature in spring, and gives suitable liquid manure sustainable growth.Lijiang spruce is sufficient at liquid manure, and giving has diauxic growth under light filling condition.If lijiang spruce not light filling late June bind, cold if light filling is processed lower day cyclostrophic, at the beginning of 8 months, also can stop growing gradually.After the high growth of lijiang spruce stops, stem and root also can continued growths, and side shoot and needle will hardening, and colour of needle can change equally.
Container seedling refers to the nursery stock that utilizes flexible container, rigid container, cave dish or mesh bag etc. to cultivate.The size of container with filling matrix after container diameter and highly represent.Conventionally cultivate 1～2 year raw nursery stock, select 5 * 10cm plastic film container, 10 * 10cm nutritive cube or 3 * 10cm compressed substrate piece container; 2～3 years raw nursery stocks, select 10 * 15cm, 10 * 18cm nutritive cube; 4～6 years raw nursery stocks are selected the container of 15 * 18cm.The disposing way of container can be frame pendulum-type and ground pendulum-type.Frame pendulum-type refers to container is closely placed on the shelf made from metal material, or on hollow brick, discarded floor; Can utilize air to be completely cured, to be beneficial to lateral root growth.Ground pendulum-type refers to closely puts container on the ground, can be concrete, brick floor file face, can be also the natural land face of compacting; Be convenient to water management and moisturizing.In the specific embodiment of the invention, First Year sowing use is 5 * 10cm plastic film container, and ground pendulum-type is put; Second Year changes 10 * 15cm or 10 * 18cm nutritive cube into, and ground pendulum-type is put.
The concrete technical scheme of the method for the invention, comprises the following steps:
(1) light filling greenhouse is built: steel arch shape plastic tunnel, do 1.2 * 2.8m seedbed, and between bed, with brick, be paved into the pavement of 24 * 250cm; Top, seedbed hangs light fixture; In canopy, have equipment of sprinkler irrigation, canopy has sunshade net fine day to control temperature noon outward.
(2) sowing: take thousand kernel weight by quartering, first use 45 ℃ of water presoaking and germinatings of beginning temperature 24 hours before sowing, change water once in every 8 hours therebetween, wet concentration seed, removes not plump kind of foreign material; Keep 25 ℃ of room temperatures; Use afterwards 0.3%(mass fraction) liquor potassic permanganate surface sterilization 30 minutes, and rinse well with clear water, then adopt conventional sawdust (1 times of volume that consumption is seed) germination hastening method to carry out vernalization; When seed has 30～40% to show money or valuables one carries unintentionally, start sowing; 3～6, each white plastic membrane container bag (5 * 10cm), covers the seedling medium about 0.5cm, and excess in the lower and deficiency in the upper, then waters permeable; Within 1 day, use prior to seeding 0.3%(mass fraction) liquor potassic permanganate to seedling medium disinfection.
Cultivating lijiang spruce container seedling seedling medium used comprises: the rural area soil that volume ratio is 3:2 and peat soil, in the rural area soil of per unit volume and peat soil (total amount of rural area soil and peat soil), add parts by weight to be the chicken manure of 10～20 parts, the phosphate fertilizer of 5～10 parts, the zinc of 0.2～0.8 part is mixed phosphorus (preferably 0.5 part), and the ferrous sulfate of 1.2～1.8 parts (preferably 1.5 parts).
In seedling medium, the content of organic matter is 8.0～8.5g/kg, is preferably 8.2g/kg; The pH value of seedling medium is 6.2～6.8, is preferably 7.5.
(3) juvenile stage and seedling management
1. topdress:
After planting First Year, every 20 days after seedling height growth starts, in the seedbed of per unit area, is executed the nitrogenous fertilizer solution that 2 parts of weight percentages are 0.3 ‰ (being foliage fertilizer) once, and within 100 days, (using 6 times) stops; Then equally every 20 days, in the seedbed of per unit area, execute potassium dihydrogen phosphate that 2 parts of weight percentages are 0.5 ‰ once, altogether use twice, promote nursery stock lignification.All use 2 parts at every turn.
After planting Second Year, every 15 days after nursery stock exhibition leaf, in the seedbed of per unit area, is executed respectively nitrogenous fertilizer solution that 3 parts of percetages by weight are 0.3 ‰ once, and within 120 days, (using 9 times) stops; Then equally every 15 days, in the seedbed of per unit area, execute potassium dihydrogen phosphate that 3 parts of percetages by weight are 0.5 ‰ once, altogether use twice, promote nursery stock lignification.All use 3 parts at every turn.
2. thinning: container seedling growth Second Year was transplanted and changed bag lijiang spruce at the beginning of 3 months, changed 10 * 18cm or 10 * 15cm nutritive cube into, every bag of strain;
3. diseases prevention: the container seedling of lijiang spruce is easily dyed damping off, after nursery stock is unearthed, sprays carbendazim, Kocide SD copper or hymexazo solution one time every blade face on the 7th and stromal surface, and three kinds of solution take turns to use;
4. weeding: juvenile stage and seedling stage weed growth vigorous, carry out in time weeding; To protect nursery stock simultaneously, not make root system impaired;
5. overwinter management: after planting at the beginning of 11 months winters of First Year, protection is survived the winter to lijiang spruce button canopy, takes off canopy April in next year; Second Year nursery stock in winter overwintering after planting.
(4) light filling is processed
After sowing lijiang spruce seed, at juvenile stage and seedling stage, container seedling is carried out to light filling processing; The light filling time comprises: from the seedling shelling of container seedling, start light filling, container seedling growth continuous light filling in season 2 years; Container seedling growth First Year starts light filling from the seedling shelling of container seedling, and the light filling period is 22:00～2:00, light filling 100～110 days; Container seedling growth Second Year starts light filling when container seedling is opened up leaf, and the light filling period is 21:00～1:00, light filling 110～120 days;
The light source that light filling is processed is: incandescent lamp, high-pressure mercury lamp, fluorescent lamp, sunlight dysprosium lamp, iodine-tungsten lamp, neon lamp or helium lamp.
As preferred embodiment, at juvenile stage and seedling stage, container seedling is carried out to light filling processing, comprising: the determining of light filling time started, light source, intensity of illumination and the selection of light filling time;
1. light filling time started: start light filling from the approximately 80% seedling shelling of container seedling;
2. light source: fluorescent lamp (emerging standing grain board, Hebei University of Science and Technology produces, power 36W, wavelength main peak 450nm and 640nm, photochromic aubergine);
When light source is fluorescent lamp, the intensity of illumination that light filling is processed: 1～6 μ molm
-2s
-1(being the scope of the suffered light intensity of nursery stock of diverse location under fluorescent lamp);
4. light filling time: container seedling growth continuous light filling in season 2 years; Container seedling growth First Year starts light filling, light filling period 22:00～2:00 from the seedling shelling of container seedling; The about 400h of light filling 100d(); Container seedling growth Second Year starts light filling at night, light filling period 21:00～1:00, light filling 120d(approximately 480 hours during from the exhibition leaf of container seedling).
In light filling process, every day, continuous light filling effect was relatively good; If the middle ware break time is longer, nursery stock is easily bound; But determining of concrete number of days, should be to stop light filling when autumn has set in, can promote like this nursery stock to bind, and should stop that nursery stock is bound and the lignified time, so that the abundant lignification of nursery stock is avoided freeze injury.
(5) go out garden
1. lifting: go out garden and container was watered in first 1～2 day permeablely, lifting again after seedling medium is slightly dry, together with plastic container bag lifting;
2. pack: classification immediately after lifting, and classification vanning;
3. transportation: entrucking when transportation, at lower cloudy day of temperature or night, carry out, transport to and scatter in time afterwards, in case nursery stock overstocks heating; Transport seedling spring and will carry out windproof, antifreeze, insulation.
Method of the present invention, implements for spring sowing specially.
Embodiment 1
Except following condition, the content of the present embodiment is identical with concrete technical scheme in above-mentioned embodiment:
In seedling raising process:
In (1) seedling medium: the rural area soil that volume ratio is 3:2 and peat soil, then according to the cumulative volume consumption of rural area soil and peat soil, in the rural area soil and peat soil of every cubic metre, the chicken manure that adds respectively 15kg, the phosphate fertilizer of 8kg, the zinc of 0.5kg is mixed phosphorus, and the ferrous sulfate that adds 1.5kg; In seedling medium, the content of organic matter is 8.2g/kg; The pH value of seedling medium is 6.5.
At (3) light filling in the time started: start light filling from the approximately 80% seedling shelling of container seedling; Light source: adopt fluorescent lamp (emerging standing grain board, Hebei University of Science and Technology produces, power 36W, wavelength main peak 450nm and 640nm, photochromic aubergine); Light filling light intensity: the position under fluorescent lamp is 6 μ molm
-2s
-1;
The light filling time: container seedling growth continuous light filling in season 2 years; After the shelling of container seedling growth First Year seedling, start light filling, light filling period 22:00～2:00, the about 400h of light filling 100d(); During 1 year year of container seedling growth exhibition leaf, start light filling at night, light filling period 21:00～1:00, the about 480h of light filling 120d().
The nursery effect of the present embodiment: the lijiang spruce container seedling Growth-Rhythm of after planting a year and 2 years is in Table 1, can draw: after planting the height of seedling mean value of 1 year provenance plik08yn018 of light filling is 7.18cm, and the height of seedling mean value of plik08yn008 is 10.87cm; After planting the height of seedling mean value of 2 years provenance plik08yn001 of light filling is 34.28cm, has reached the standard in the garden of afforesting out, compares to have shifted to an earlier date 3～4 years and go out garden with traditional seedling growing process.
The standard in the above-mentioned garden of afforesting out is specially: nursery stock is up to 20～30cm, and seedling is dry perfectly straight, grows fine, and has no mechanical damage and damage by disease and insect.Above-mentioned traditional seedling growing process is specially: open country is grown seedlings, and bare area is grown seedlings, and without container, does not also supplement illumination.
Table 1: lijiang spruce container seedling Growth-Rhythm
After planting the 3rd year, investigated the storage rate of 20 provenance container seedlings of lijiang spruce in March, 2010, and randomly draw wherein their average height of seedling of seedling survey and the leading thread of 60 strain container seedlings in 18 provenances, each provenance, the results are shown in Table 2.The average storage rate of 20 provenance container seedlings of lijiang spruce reaches 96.33%, average height of seedling 54.81cm, and average leading thread is 8.50mm.If adopt prior art to grow seedlings, after planting the about 10cm of the 3rd annual height of seedling.
Therefore, adopt after the inventive method, compared to the prior art, average height of seedling exceeds approximately 4.5 times.Above-mentioned prior art is specially: traditional seedling growing process, and open country is grown seedlings, and bare area is grown seedlings, and without container, does not also supplement illumination.
Show 2:20 provenance lijiang spruce container seedling forestation census list
In the embodiment of the present invention, lijiang spruce provenance is drawn mountain, Gansu Province dragon spruce germplasm resource bank from childhood.
Embodiment 2
Other conditions are with embodiment 1, and difference is, in seedling raising process:
In (1) seedling medium: the rural area soil that volume ratio is 3:2 and peat soil, then according to the cumulative volume consumption of rural area soil and peat soil, in the rural area soil and peat soil of every cubic metre, the chicken manure that adds respectively 10kg, the phosphate fertilizer of 10kg, the zinc of 0.8kg is mixed phosphorus, and the ferrous sulfate that adds 1.2kg; In seedling medium, the content of organic matter is 8.0g/kg; The pH value of seedling medium is 6.8.
Embodiment 3
Other conditions are with embodiment 1, and difference is, in seedling raising process:
In (1) seedling medium: the rural area soil that volume ratio is 3:2 and peat soil, then according to the cumulative volume consumption of rural area soil and peat soil, in the rural area soil and peat soil of every cubic metre, the chicken manure that adds respectively 20kg, the phosphate fertilizer of 5kg, the zinc of 0.2kg is mixed phosphorus, and the ferrous sulfate that adds 1.8kg; In seedling medium, the content of organic matter is 8.5g/kg; The pH value of seedling medium is 6.2.
The nursery effect of other embodiments of the invention and embodiment's 1 is similar, more excellent with embodiment 1.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. a light filling seedling-cultivating method for lijiang spruce container seedling, comprising: after sowing lijiang spruce seed, container seedling is carried out to light filling processing; The light filling time comprises: from the seedling shelling of container seedling, start light filling, container seedling growth continuous light filling in season 2 years;
Container seedling growth First Year starts light filling from the seedling shelling of container seedling, and the light filling period is 22:00～2:00, light filling 100～110 days; Container seedling growth Second Year starts light filling when container seedling is opened up leaf, and the light filling period is 21:00～1:00, light filling 110～120 days.
2. method according to claim 1, is characterized in that, container seedling growth First Year light filling 100 days, container seedling growth Second Year light filling 120 days; From container seedling 80% seedling shelling, start light filling.
3. method according to claim 1 and 2, is characterized in that, the light source that light filling is processed is: incandescent lamp, high-pressure mercury lamp, fluorescent lamp, sunlight dysprosium lamp, iodine-tungsten lamp, neon lamp or helium lamp.
4. method according to claim 3, is characterized in that, the light source that light filling is processed is fluorescent lamp, and the intensity of illumination that light filling is processed is 1～6 μ molm
-2s
-1.
5. according to the method described in claim 3 or 4, it is characterized in that, the light source that light filling is processed is fluorescent lamp, and its spectral wavelength scope is 400～700nm.
6. according to the method described in claim 1～5 any one, it is characterized in that, cultivate lijiang spruce container seedling seedling medium used, comprising: the rural area soil that volume ratio is 3:2 and peat soil, wherein add chicken manure, phosphate fertilizer, zinc to mix p and s acid ferrous iron again;
In the rural area soil and peat soil of per unit volume, chicken manure, phosphate fertilizer, zinc are mixed the ferrous parts by weight of p and s acid and are respectively: 10～20 parts of chicken manures, and 5～10 parts, phosphate fertilizer, zinc is mixed 0.2～0.8 part, phosphorus, and zinc is mixed preferably 0.5 part, phosphorus; 1.2～1.8 parts, ferrous sulfate, preferably 1.5 parts, ferrous sulfate;
In seedling medium, the content of organic matter is 8.0～8.5g/kg, preferably 8.2g/kg; The pH value of seedling medium is 6.2～6.8, preferably 6.5;
1 day liquor potassic permanganate that is 0.3% with quality percentage composition is to described seedling medium disinfection prior to seeding.
7. according to the method described in claim 1～6 any one, it is characterized in that, lijiang spruce seed prior to seeding, also comprises: with 45 ℃ of water presoaking and germinatings 24 hours, after wet concentration seed, remain on 25 ℃; By quality, dividing percentage composition is afterwards 0.3% disinfecting solution of potassium permanganate 30 minutes, and rinses well with clear water, with volume, is then the sawdust vernalization of 1 times of seed volume; When seed has 30～40% to show money or valuables one carries unintentionally, start sowing.
8. according to the method described in claim 1～7 any one, it is characterized in that, the method also comprises the management of the container seedling of lijiang spruce being carried out to juvenile stage and seedling stage, comprising: topdress and diseases prevention;
Described fertilizing type comprises: after planting First Year, every 20 days after seedling height growth starts, in the seedbed of per unit area, is used nitrogenous fertilizer solution that 2 parts of weight percentages are 0.3 ‰ once, uses altogether 6 times; Then use potassium dihydrogen phosphate that 2 parts of weight percentages are 0.5 ‰ once, use altogether twice;
After planting Second Year, every 15 days after nursery stock exhibition leaf, in the seedbed of per unit area, is used respectively nitrogenous fertilizer solution that 3 parts of percetages by weight are 0.3 ‰ once, uses altogether 9 times; Then use potassium dihydrogen phosphate that 3 parts of percetages by weight are 0.5 ‰ once, use altogether twice;
Described diseases prevention mode comprises: after nursery stock is unearthed, every 7 days, to blade face and stromal surface, spray carbendazim, Kocide SD or hymexazo solution one time.
9. the application of the method described in claim 1～8 any one in cultivating lijiang spruce container seedling.
10. application according to claim 9, is characterized in that, the seed of lijiang spruce comes from Shangri-La, Yunnan Province county.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310554487.3A CN103636374B (en) | 2013-11-08 | 2013-11-08 | The light filling seedling-cultivating method of lijiang spruce container seedling and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310554487.3A CN103636374B (en) | 2013-11-08 | 2013-11-08 | The light filling seedling-cultivating method of lijiang spruce container seedling and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103636374A true CN103636374A (en) | 2014-03-19 |
| CN103636374B CN103636374B (en) | 2015-08-05 |
Family
ID=50241792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310554487.3A Expired - Fee Related CN103636374B (en) | 2013-11-08 | 2013-11-08 | The light filling seedling-cultivating method of lijiang spruce container seedling and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103636374B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585005A (en) * | 2015-01-07 | 2015-05-06 | 浙江省萧山棉麻研究所 | Light-supplementing cultivation method for promoting fast growth of acer palmatum container seedlings |
| CN104429555B (en) * | 2014-12-19 | 2016-08-24 | 中国科学院东北地理与农业生态研究所 | A kind of light filling breeding method promoting Ramulus et folium taxi cuspidatae container seedling fast-growing |
| CN106508575A (en) * | 2016-11-10 | 2017-03-22 | 唐翔 | Keteleeria pubescens seedling raising method |
| CN106717888A (en) * | 2016-12-09 | 2017-05-31 | 江西邓志平园林绿化有限公司 | A kind of breeding method of the annual container seedling of monkey camphor tree |
| CN107396792A (en) * | 2017-06-28 | 2017-11-28 | 安徽大自然苗木科技有限公司 | A kind of short strong method for cultivating seedlings |
| CN108811870A (en) * | 2018-04-12 | 2018-11-16 | 中国林业科学研究院林业研究所 | A kind of spectrum being adapted to European spruce growth of seedling and proportion |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2468090A1 (en) * | 2010-12-21 | 2012-06-27 | Valoya Oy | Method and means for acclimatizing seedlings for outdoor life |
-
2013
- 2013-11-08 CN CN201310554487.3A patent/CN103636374B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2468090A1 (en) * | 2010-12-21 | 2012-06-27 | Valoya Oy | Method and means for acclimatizing seedlings for outdoor life |
Non-Patent Citations (7)
| Title |
|---|
| 刘林英等: "蓝云杉快速育苗技术", 《林业实用技术》, no. 05, 15 May 2012 (2012-05-15), pages 22 - 23 * |
| 杨海裕等: "补充光照对欧洲云杉苗木生长的影响", 《甘肃林业科技》, no. 01, 30 March 2006 (2006-03-30) * |
| 欧阳芳群等: "补光处理对黑云杉不同种源苗木生长的影响", 《北京林业大学学报》, no. 05, 15 September 2010 (2010-09-15), pages 82 - 87 * |
| 王军辉等: "云杉强化育苗技术的研究现状和展望", 《浙江林学院学报》, no. 03, 30 June 2005 (2005-06-30) * |
| 王志涛: "白云杉补光育苗试验", 《林业科技》, no. 05, 25 September 2006 (2006-09-25) * |
| 蒋明等: "补光对北美兰云杉育苗的影响", 《甘肃农业科技》, no. 05, 20 May 2008 (2008-05-20) * |
| 赵文英等: "7个云杉树种1年生苗对补光的响应", 《甘肃林业科技》, no. 01, 15 March 2011 (2011-03-15) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104429555B (en) * | 2014-12-19 | 2016-08-24 | 中国科学院东北地理与农业生态研究所 | A kind of light filling breeding method promoting Ramulus et folium taxi cuspidatae container seedling fast-growing |
| CN104585005A (en) * | 2015-01-07 | 2015-05-06 | 浙江省萧山棉麻研究所 | Light-supplementing cultivation method for promoting fast growth of acer palmatum container seedlings |
| CN106508575A (en) * | 2016-11-10 | 2017-03-22 | 唐翔 | Keteleeria pubescens seedling raising method |
| CN106717888A (en) * | 2016-12-09 | 2017-05-31 | 江西邓志平园林绿化有限公司 | A kind of breeding method of the annual container seedling of monkey camphor tree |
| CN107396792A (en) * | 2017-06-28 | 2017-11-28 | 安徽大自然苗木科技有限公司 | A kind of short strong method for cultivating seedlings |
| CN108811870A (en) * | 2018-04-12 | 2018-11-16 | 中国林业科学研究院林业研究所 | A kind of spectrum being adapted to European spruce growth of seedling and proportion |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103636374B (en) | 2015-08-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103039254B (en) | Seedling growing method of apocarya | |
| CN102939880B (en) | A kind of black plum standardized planting method | |
| CN101816251B (en) | A kind of cutting propagation method of tetraploid Robinia pseudoacacia | |
| CN103988697B (en) | A kind of method of apocarya seedling breeding | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN104145794B (en) | A kind of dendrobe tissue culture seedling heel in hardening off method | |
| CN103283450B (en) | Golden camellia bud seedling stock grafting propagation method | |
| CN104429581B (en) | A kind of Organic tomato health cultural method | |
| CN105393892B (en) | Appoint beans and dalbergia odorifera Mixed forestation method in Rocky Desertification Region | |
| CN102113445B (en) | Method for raising seedlings of Pinus kesiya var.langbianensis by efficient twig cuttage propagation | |
| CN1748462A (en) | A kind of cuttage propagation method of Lonicera indigo Lonicerae green branches | |
| CN103636374A (en) | Light supplement seedling culture method for Lijiang spruce container seedlings and application thereof | |
| CN106472121A (en) | A kind of cultural method viewing and admiring the multiple Kiwifruit Cultivars of potted plant Fructus actinidiae chinensiss grafting | |
| CN105454018A (en) | Cultivation method for echeveria succulent plants | |
| CN104145795A (en) | Dendrobe cultivation method for improving facility utilization rate | |
| CN105918089A (en) | Soilless planting method for organic strawberries | |
| CN104429785A (en) | High-quality and efficient golden bell tree seedling growing method | |
| CN102318546A (en) | Method for growing container seedlings of cork oak in winter | |
| CN103583346A (en) | Soilless cultivation method for citrus bergamot | |
| CN102550271B (en) | Method for commercialized cultivation of container seedling of cold-resistant japanese cinnamon germplasm | |
| CN102792831B (en) | High-efficiency rapid propagation technique for Chinese yew | |
| CN107278769A (en) | It is a kind of to promote the beautiful seedling of hybridization with a smile to do sth. in advance the method bloomed | |
| CN103430744A (en) | Method for rapid breeding of fritillaria cirrhosa by means of fritillaria cirrhosa scales | |
| CN106613659B (en) | Grafting cultivation method of euonymus plant golden beetle | |
| CN102960247A (en) | Method for obtaining China fir tissue cultured explants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150805 Termination date: 20191108 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |