CN103614859A - Method for preparing glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane - Google Patents
Method for preparing glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane Download PDFInfo
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Abstract
The invention relates to a method for preparing a glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane. The method comprises the steps that sebacic acid divinyl ester and glucose are dissolved in anhydrous pyridine, alkaline protease is added, separation and purification are conducted, and glucose ethylene ester is obtained; N-isopropylacrylamide, an initiator and a solvent are added in the glucose ethylene ester, then a stirring reaction is conducted under the protection of nitrogen, and glucose divinyl ester/N-isopropylacrylamide copolymer is obtained after a polymerization reaction is completed; the copolymer and PLCL are added in the solvent to obtain a spinning solution, then electrostatic spinning is conducted, and the glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane can be obtained after drying. According to the method for preparing the glucose sebacic acid divinyl ester/N-isopropylacrylamide copolymer nanofiber membrane, operating is simple, products are easy to process, and economic and environmentally friendly, the obtained nanofiber membrane contains rich reacting hydrophilic active functional groups and has the phase-transition temperature close to body temperature, the specific adsorption of the surfaces of materials is improved, the biocompatibility of the surfaces of the materials is improved, and the nanofiber membrane can be used as an intelligent drug release carrier on the basis of phase transition properties.
Description
Technical field
The invention belongs to the preparation field of nano fibrous membrane, particularly a kind of preparation method of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa.
Background technology
Sugar and protein, lipid is the same with nucleic acid, is the important component that forms organism.The glycoconjugate extensively existing, comprises glycoprotein, and proteoglycans and glycolipid have participated in the target of albumen, cell recognition, the important physiology course such as antibody-AI.Glycosylation film has high hydrophily and anti-non-specific protein absorption and the high characteristics such as biocompatibility, therefore be widely used in very much protein separation, the specificity of bacterium catches, the aspects such as cultivation of bio-medical material and cell, can also prepare surface-functionalized diffusion barrier.
NIPA is owing to having hydrophilic amide groups and hydrophobic isopropyl in molecule, its homogeneous polymer has the good characteristics such as lower critical solution temperature (LCST).Because its polymer has at 32 ℃, be above this special dissolution characteristics of water-insoluble, developing the raw material for the manufacture of some response to temperature polymer gel.Easily polymerization separately of common radical polymerization initiator for NIPA, also easily copolymerization.Therefore, it becomes the focus of current thermoresponsive investigation of materials.
Utilize sugary nano fibrous membrane prepared by electrostatic spinning technique to there are the potentiality of specific adsorption mechanism between follow-up study protein.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa, the method is simple to operate, product is easily processed and economic environmental protection, this nano fibrous membrane contain abundant can reactive hydrophilic activated functional groups and there is the phase transition temperature close to body temperature, when having improved the specific adsorption of material surface and having improved the biocompatibility of material surface, on the basis of phase-change characteristic, can there be a lot of potential application.
The preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa of the present invention, comprising:
(1) the decanedioic acid divinyl fat that is 1-4:1 by mol ratio and glucose are dissolved in anhydrous pyridine, add alkali protease, under 40-60 ℃ of condition, react 3-5 days, and separating-purifying obtains glucose ethene fat; Wherein the amount ratio of alkali protease and anhydrous pyridine is 0.5-1.5g:50-100mL;
(2) in above-mentioned glucose ethene fat, add NIPA, initator and solvent, then stirring reaction 3-8h under 55-60 ℃ of nitrogen protection, obtains glucose ethene fat/N-isopropylacrylamide copolymer after polymerisation finishes; Wherein the mol ratio of NIPA and glucose ethene fat is 1-50:1, and initator accounts for the 0.2-2% of glucose ethene fat and NIPA quality sum;
(3) above-mentioned glucose ethene fat/N-isopropylacrylamide copolymer and PLCL are added in solvent, stir to obtain glucose ethene fat/N-isopropylacrylamide copolymer spinning fluid, then carry out electrostatic spinning, be drying to obtain glucose ethene fat/N-isopropylacrylamide copolymer nano tunica fibrosa, wherein to account for the mass percent of spinning solution be 3-30% for glucose ethene fat/N-isopropylacrylamide copolymer and PLCL sum, and the mol ratio of glucose ethene fat/N-isopropylacrylamide copolymer and PLCL is 1:1.
In described step (1), reaction is carried out in constant-temperature shaking incubator, and rotating speed is 200rpm.
In described step (1), separating-purifying is: use silica gel column chromatography separating-purifying, eluant, eluent is ethyl acetate, and solvent is that volume ratio is ethyl acetate, the first alcohol and water of 17:3:1, uses I
2colour developing.
In described step (2), initator is azodiisobutyronitrile AIBN, and solvent is absolute ethyl alcohol, and glucose ethene fat and the NIPA concentration sum in ethanol solution is 2-3mol/L.
After polymerisation finishes in described step (2), product is removed to the no glucose ethene fat of reaction through 3500D dialysis membrane.
Solvent in described step (3) is absolute ethyl alcohol.
In described step (3), in glucose ethene fat/N-isopropylacrylamide copolymer, the mol ratio of glucose ethene fat and N-isopropylacrylamide is 1:5-1:25.
In described step (3), electrostatic spinning process parameter is: specification of syringe is 5mL, and syringe needle internal diameter is 0.4-0.7mm, ejection flow velocity 0.8-2mL/h, electrostatic pressure 10-18kV, receiving screen adopts the reception of aluminium foil ground connection, and accepting distance is 10-20cm, adopts orthogonal method to regulate spinning parameter to carry out electrospinning.
In described step (3), baking temperature is 30-60 ℃, and be 24-48h drying time.
Glucose ethene fat/N-isopropylacrylamide copolymer nano tunica fibrosa of described step (3) gained is applied to separation and purification, research carbohydrate and the protein specific adsorption mechanism of protein.
In the present invention, the preparation method of decanedioic acid divinyl fat used is as follows:
By the decanedioic acid of certain molar weight, vinylacetate (1:1～1:10mol/mol), 1g～5g mercuric acetate, acetate in minute copper is mixed in 250mL flask, after stirring at room 1～15min, add the 0.1-0.5mL concentrated sulfuric acid, constant temperature stirring reaction 8h at 50 ℃～70 ℃.React the complete blue settled solution that obtains.After reactant liquor is cooling, add 0.1～1.5g anhydrous sodium acetate, fully concussion is to remove sulfuric acid wherein, and after standing filtration, rotary evaporation is removed excessive vinylacetate.Surplus solution, through silica gel column chromatography (300～400 order) separating-purifying, obtains decanedioic acid divinyl fat.
The application of glycosyl modified temperature-sensitive nano fiber in protein identification.
Glycosyl modified acrylonitrile group nano fibre film is immersed in same concentrations FITC-Con A and RBITC-BSA solution, and concussion absorption is 2 hours at 37 ℃, leaches, and cleans.Utilize the specific adsorption effect of glycosyl and specified protein, nanofiber is adsorbed proteins optionally, protein can be eluted from fiber, thereby realize the separation of protein in mixed liquor with the sugar juice of high concentration.
Sugar is a kind of in biomolecule, is extensively present in organism, and be polyhydroxyl compound, there is extremely strong hydrophily, therefore, sugar is introduced to the surface of material, will effectively improve the biocompatibility of material surface.And, carbohydrate can with agglutinin specific recognition, can increase the absorption between material and specific protein.
Thermo-sensitive is containing the application of sugar copolymer in oncotherapy.
Thermo-sensitive material is introduced containing in sugar substance, can realize the Thermo-sensitive of material is controlled, it and hydrophobic high molecular block copolymer are often used to prepare micella pharmaceutical carrier, can make the LCST of copolymer reach 37 ℃ even higher, by after itself and aliphatic polyester block copolymerization and be prepared into carrier micelle, in conjunction with hot-spot processing method, can realize the temperature sensitive control of tumor tissues is discharged.And proof on evidence, Con A has the functions such as the adhesion of regulate tumor cell and Apoptosis, therefore, can utilize the specific adsorption character of material to Con A, for the apoptosis-promoting effect to tumour cell.
The present invention carries out electrospinning by NIPA and the copolymerization of glucose ethene fat, obtains diameter at nanoscale glucose ethene fat/N-isopropylacrylamide copolymer nano tunica fibrosa.This membrane material contain abundant can reactive hydrophilic activated functional groups, improve the non-specific adsorption of material surface and improved the biocompatibility of material surface, it does the potentiality that follow-up related experiment is analyzed to have application, and there is controlled LCST value, there is the application potential such as target intelligence release grade of realizing medicine.Beneficial effect:
(1) the present invention adopts aqueous phase precipitation polymerization to prepare glucose ethene resin copolymer, and preparation method is simple to operate, product is easily processed and economic environmental protection;
(2) in the present invention, NIPA copolymer nano fiber is a class specific surface, the carrier material of high porosity;
(3) membrane material of the present invention contain abundant can reactive hydrophilic activated functional groups and there is the phase transition temperature close to body temperature, the glucosyl group that can react is contained on membrane material surface, can and specific agglutinin between there is identification mutually, therefore improve the specific adsorption ability of material surface and improved the biocompatibility of material surface, it does the potentiality that follow-up related experiment is analyzed to have application, on the basis of phase-change characteristic, can there be a lot of potential application, as realized by hot-spot processing method, to the intelligence of tumor tissues, temperature sensitive control discharges, also can prepare the tissue engineering bracket with phase transformation,
(4) in the present invention, glycosylation nanofiber is for the specific recognition of protein, efficient, fast, is conducive to study the mechanism of specific recognition between carbohydrate and protein, and can be repeatedly used, and is easy to suitability for industrialized production.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph (spinning voltage is 10kV, and receiving range is 15cm, and injection flow velocity is 1.0mL/h) of the sugary copolymer nano tunica fibrosa of Thermo-sensitive of embodiment 1-3 gained;
Fig. 2 is the Con A of embodiment 1-3 under 37 ℃ of conditions and the CLSM picture of BSA absorption, wherein glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA of A-a: embodiment 1 preparation; Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of B-a: embodiment 1 preparation are attached to the desorption of Con A and BSA; Glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA of A-b: embodiment 2 preparations; Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of B-b: embodiment 2 preparations are attached to the desorption of Con A and BSA; Glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA of A-c: embodiment 3 preparations; Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of B-c: embodiment 3 preparations are attached to the desorption of Con A and BSA.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
(1) by decanedioic acid divinyl fat and glucose according to a certain percentage (4:1, mol ratio) be dissolved in anhydrous pyridine, utilize enzymatic synthetic technology synthesis of glucose ethene fat.After reaction finishes, crude product silica gel column chromatography separating-purifying, eluant, eluent is ethyl acetate, solvent is ethyl acetate, first alcohol and water (17:3:1, v/v/v), uses I
2colour developing.Above-mentioned glycolipid is put in micro-polymerization pipe, add a certain amount of NIPA (with the mol ratio of glucose ethene fat be 7:1), with azodiisobutyronitrile (AIBN) (mass fraction that accounts for glucose ethene fat and NIPA gross mass is 2.0) as initator, add absolute ethyl alcohol to make solvent (monomer concentration is 2.5mol/L absolute ethyl alcohol), sealing, with oil pump, vacuumize rear logical nitrogen, repeated multiple times.System is placed in to stirring reaction 6h under 50-60 ℃ of nitrogen protection.
(2) by the Thermo-sensitive glucose ethene resin copolymer of certain mass and PLCL, according to mol ratio, be that 1:1 adds in absolute ethyl alcohol, in copolymer, the mol ratio of glucose lipid and NIPAM is 1:10, stirred for several hour is extremely dissolved completely, standing a few hours disappear bubble in spinning solution completely, stir and form homogeneous spinning solution.
(3) with syringe, extract Thermo-sensitive containing sugar copolymer, be fixed on electrostatic spinning apparatus, control ejection flow velocity 1.0ml/h, electrostatic pressure 10kV, receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10-20cm, adopts orthogonal method (to change a certain parameter, fix other parameter) regulate different spinning parameters to carry out electrospinning, obtain Thermo-sensitive containing sugar copolymer superfine nano tunica fibrosa; Put into vacuum drying chamber 30-60 ℃ of freeze-day with constant temperature 36h, make regeneration nano composite membrane, its electromicroscopic photograph as shown in Figure 1A.
(4) glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA
Preparation HEPES cushioning liquid, pH is 7.5,10mM HEPES wherein, 0.15M NaCl, 0.1mM Ca
2+, 0.01mM Mn
2+.
Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of embodiment 1 preparation are successively used to PBS cushioning liquid preliminary treatment (20-30min), with the pollutant except striping surface, get 0.5mg film to be measured, after shredding, put into test tube, the FITC-Con A and the RBITC-BSA solution that add respectively 1-10mL same concentrations, at 37 ℃ of round waters constant temperature oscillator lucifuge constant temperature 2h.After balance upon adsorption, take out film, with HEPES cushioning liquid, clean 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 A-a.
(5) glucose ethene fat/N-isopropylacrylamide copolymer and PLCL blending nano fibrous membrane are attached to the desorption of Con A and BSA
The membrane material of above-mentioned absorption FITC-Con A and RBITC-BSA is immersed in the glucose solution that the concentration of 200 μ L is 1M to the attached 24h of desorption under 37 ℃ of conditions.Then, HEPES for membrane material is cleaned 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 B-a.
Embodiment 2
(1) by decanedioic acid divinyl fat and glucose according to a certain percentage (4:1, mol ratio) be dissolved in anhydrous pyridine, utilize enzymatic synthetic technology synthesis of glucose ethene fat.After reaction finishes, crude product silica gel column chromatography separating-purifying, eluant, eluent is ethyl acetate, solvent is ethyl acetate, first alcohol and water (17:3:1, v/v/v), uses I
2colour developing.Add a certain amount of NIPA (with the mol ratio of glucose ethene fat be 7:1), with azodiisobutyronitrile (AIBN) (mass fraction that accounts for glucose ethene fat and NIPA gross mass is 2.0) as initator, add absolute ethyl alcohol to make solvent (monomer concentration is 2.5mol/L absolute ethyl alcohol), sealing, with oil pump, vacuumize rear logical nitrogen, repeated multiple times.System is placed in to stirring reaction 6h under 55-60 ℃ of nitrogen protection.
(2) by the Thermo-sensitive glucose ethene resin copolymer of certain mass and PLCL, according to mol ratio, be that 1:1 adds in absolute ethyl alcohol, in copolymer, the mol ratio of glucose lipid and NIPAM is 1:15, stirred for several hour is extremely dissolved completely, standing a few hours disappear bubble in spinning solution completely, stir and form homogeneous spinning solution.
(3) with syringe, extract Thermo-sensitive containing sugar copolymer, be fixed on electrostatic spinning apparatus, control ejection flow velocity 1.0ml/h, electrostatic pressure 10kV, receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10-20cm, adopts orthogonal method (to change a certain parameter, fix other parameter) regulate different spinning parameters to carry out electrospinning, obtain Thermo-sensitive containing sugar copolymer superfine nano tunica fibrosa; Put into vacuum drying chamber 60-80 ℃ of freeze-day with constant temperature 24h, make regeneration nano composite membrane, its electromicroscopic photograph as shown in Figure 1B.
(4) glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA
Preparation HEPES cushioning liquid, pH is 7.5,10mM HEPES wherein, 0.15M NaCl, 0.1mM Ca
2+, 0.01mM Mn
2+.
Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of embodiment 2 preparations are successively used to PBS cushioning liquid preliminary treatment (20-30min), with the pollutant except striping surface, get 0.5mg film to be measured, after shredding, put into test tube, the FITC-Con A and the RBITC-BSA solution that add respectively 1-10mL same concentrations, at 37 ℃ of round waters constant temperature oscillator lucifuge constant temperature 2h.After balance upon adsorption, take out film, with HEPES cushioning liquid, clean 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 A-b.
(5) glucose ethene fat/N-isopropylacrylamide copolymer and PLCL blending nano fibrous membrane are attached to the desorption of Con A and BSA
The membrane material of above-mentioned absorption FITC-Con A and RBITC-BSA is immersed in the glucose solution that the concentration of 200 μ L is 1M to the attached 24h of desorption under 37 ℃ of conditions.Then, HEPES for membrane material is cleaned 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 B-b.
Embodiment 3
(1) by decanedioic acid divinyl fat and glucose according to a certain percentage (4:1, mol ratio) be dissolved in anhydrous pyridine, utilize enzymatic synthetic technology synthesis of glucose ethene fat.After reaction finishes, crude product silica gel column chromatography separating-purifying, eluant, eluent is ethyl acetate, solvent is ethyl acetate, first alcohol and water (17:3:1, v/v/v), uses I
2colour developing.Above-mentioned glycolipid is put in micro-polymerization pipe, add a certain amount of NIPA (with the mol ratio of glucose ethene fat be 7:1), with azodiisobutyronitrile (AIBN) (mass fraction that accounts for glucose ethene fat and NIPA gross mass is 2.0) as initator, add absolute ethyl alcohol to make solvent (monomer concentration is 2.5mol/L absolute ethyl alcohol), sealing, with oil pump, vacuumize rear logical nitrogen, repeated multiple times.System is placed in to stirring reaction 6h under 50-60 ℃ of nitrogen protection.
(2) by the Thermo-sensitive glucose ethene resin copolymer of certain mass and PLCL, according to mol ratio, be that 1:1 adds in absolute ethyl alcohol, in copolymer, the mol ratio of glucose lipid and NIPAM is 1:20, stirred for several hour is extremely dissolved completely, standing a few hours disappear bubble in spinning solution completely, stir and form homogeneous spinning solution.
(3) with syringe, extract Thermo-sensitive containing sugar copolymer, be fixed on electrostatic spinning apparatus, control ejection flow velocity 1.0ml/h, electrostatic pressure 10kV, receiving screen adopts the reception of aluminium foil ground connection, and the distance of syringe needle and receiving screen is 10-20cm, adopts orthogonal method (to change a certain parameter, fix other parameter) regulate different spinning parameters to carry out electrospinning, obtain Thermo-sensitive containing sugar copolymer superfine nano tunica fibrosa; Put into vacuum drying chamber 60-80 ℃ of freeze-day with constant temperature 24h, make regeneration nano composite membrane, its electromicroscopic photograph as shown in Figure 1 C.
(4) glucose ethene fat/N-isopropylacrylamide copolymer and the absorption of PLCL blending nano fibrous membrane to Con A and BSA
Preparation HEPES cushioning liquid, pH is 7.5,10mM HEPES wherein, 0.15M NaCl, 0.1mM Ca
2+, 0.01mM Mn
2+.
Glucose ethene fat/N-isopropylacrylamide copolymer and the PLCL blending nano fibrous membrane of embodiment 3 preparations are successively used to PBS cushioning liquid preliminary treatment (20-30min), with the pollutant except striping surface, get 0.5mg film to be measured, after shredding, put into test tube, the FITC-Con A and the RBITC-BSA solution that add respectively 1-10mL same concentrations, at 37 ℃ of round waters constant temperature oscillator lucifuge constant temperature 2h.After balance upon adsorption, take out film, with HEPES cushioning liquid, clean 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 A-c.
(5) glucose ethene fat/N-isopropylacrylamide copolymer and PLCL blending nano fibrous membrane are attached to the desorption of Con A
The membrane material of above-mentioned absorption FITC-Con A and RBITC-BSA is immersed in the glucose solution that the concentration of 200 μ L is 1M to the attached 24h of desorption under 37 ℃ of conditions.Then, HEPES for membrane material is cleaned 4-8 time, after at room temperature vacuumize.After dry end, utilize CLSM to measure the fluorescence intensity of material surface.As Fig. 2 B-c.
Claims (10)
1. a preparation method for glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa, comprising:
(1) the decanedioic acid divinyl fat that is 1-4:1 by mol ratio and glucose are dissolved in anhydrous pyridine, add alkali protease, under 40-60 ℃ of condition, react 3-5 days, and separating-purifying obtains glucose ethene fat; Wherein the amount ratio of alkali protease and anhydrous pyridine is 0.5-1.5g:50-100mL;
(2) in above-mentioned glucose ethene fat, add NIPA, initator and solvent, then stirring reaction 3-8h under 55-60 ℃ of nitrogen protection, obtains glucose ethene fat/N-isopropylacrylamide copolymer after polymerisation finishes; Wherein the mol ratio of NIPA and glucose ethene fat is 1-50:1, and initator accounts for the 0.2-2% of glucose ethene fat and NIPA quality sum;
(3) above-mentioned glucose ethene fat/N-isopropylacrylamide copolymer and PLCL are added in solvent, stir to obtain glucose ethene fat/N-isopropylacrylamide copolymer spinning fluid, then carry out electrostatic spinning, be drying to obtain glucose ethene fat/N-isopropylacrylamide copolymer nano tunica fibrosa, wherein to account for the mass percent of spinning solution be 3-30% for glucose ethene fat/N-isopropylacrylamide copolymer and PLCL sum, and the mol ratio of glucose ethene fat/N-isopropylacrylamide copolymer and PLCL is 1:1.
2. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, is characterized in that: in described step (1), reaction is carried out in constant-temperature shaking incubator, and rotating speed is 200rpm.
3. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, it is characterized in that: in described step (1), separating-purifying is: use silica gel column chromatography separating-purifying, eluant, eluent is ethyl acetate, solvent is that volume ratio is ethyl acetate, the first alcohol and water of 17:3:1, uses I
2colour developing.
4. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, it is characterized in that: in described step (2), initator is azodiisobutyronitrile AIBN, solvent is absolute ethyl alcohol, and glucose ethene fat and the NIPA concentration sum in ethanol solution is 2-3mol/L.
5. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, it is characterized in that: after polymerisation finishes in described step (2), product is removed to the no glucose ethene fat of reaction through 3500D dialysis membrane.
6. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, is characterized in that: the solvent in described step (3) is absolute ethyl alcohol.
7. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, it is characterized in that: in described step (3), in glucose ethene fat/N-isopropylacrylamide copolymer, the mol ratio of glucose ethene fat and N-isopropylacrylamide is 1:5-1:25.
8. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, it is characterized in that: in described step (3), electrostatic spinning process parameter is: specification of syringe is 5mL, syringe needle internal diameter is 0.4-0.7mm, ejection flow velocity 0.8-2mL/h, electrostatic pressure 10-18kV, receiving screen adopts the reception of aluminium foil ground connection, and accepting distance is 10-20cm, adopts orthogonal method to regulate spinning parameter to carry out electrospinning.
9. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, is characterized in that: in described step (3), baking temperature is 30-60 ℃, and be 24-48h drying time.
10. the preparation method of a kind of glucose decanedioic acid divinyl fat/N-isopropylacrylamide copolymer nano tunica fibrosa according to claim 1, is characterized in that: glucose ethene fat/N-isopropylacrylamide copolymer nano tunica fibrosa of described step (3) gained is applied to the separation and purification of protein.
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| CN103173934A (en) * | 2013-04-12 | 2013-06-26 | 东华大学 | Preparation method of galactose vinyl ester/isopropyl acrylamide copolymer nanofiber membrane |
| CN103173933A (en) * | 2013-04-12 | 2013-06-26 | 东华大学 | Preparation method of glucose vinyl ester/isopropyl acrylamide copolymer nanofiber membrane |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2001214357A (en) * | 2000-02-04 | 2001-08-07 | Mitsubishi Rayon Co Ltd | Formed article made from polysaccharides and method of producing the polysaccharide formed article |
| WO2010083530A2 (en) * | 2009-01-16 | 2010-07-22 | Zeus Industrial Products, Inc. | Electrospinning of ptfe with high viscosity materials |
| CN102659979A (en) * | 2012-05-11 | 2012-09-12 | 东华大学 | Preparation method of double-hydrophilic temperature response polymer |
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| CN103173934A (en) * | 2013-04-12 | 2013-06-26 | 东华大学 | Preparation method of galactose vinyl ester/isopropyl acrylamide copolymer nanofiber membrane |
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