CN103609833A - Processing method of potato protein peptide - Google Patents

Processing method of potato protein peptide Download PDF

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Publication number
CN103609833A
CN103609833A CN201310577075.1A CN201310577075A CN103609833A CN 103609833 A CN103609833 A CN 103609833A CN 201310577075 A CN201310577075 A CN 201310577075A CN 103609833 A CN103609833 A CN 103609833A
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CN
China
Prior art keywords
potato protein
minutes
processing
protein peptide
bacillus subtilis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310577075.1A
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Chinese (zh)
Inventor
赵云财
佟晓芳
李娜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HARBIN AIKEER FOOD TECHNOLOGY Co Ltd
Original Assignee
HARBIN AIKEER FOOD TECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HARBIN AIKEER FOOD TECHNOLOGY Co Ltd filed Critical HARBIN AIKEER FOOD TECHNOLOGY Co Ltd
Priority to CN201310577075.1A priority Critical patent/CN103609833A/en
Publication of CN103609833A publication Critical patent/CN103609833A/en
Pending legal-status Critical Current

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Abstract

The invention provides a processing method of potato protein peptide. The processing method comprises the following steps: with potato protein powder as a raw material, adding water, mixing until the concentration of the raw material is 10%-15%, adjusting the pH value to be 6.5-7.0, sterilizing at 121 DEG C for 30 minutes, cooling to 30 DEG C, inoculating bacillus subtilis liquid strain accounting for 8%-10% of the total quantity of a fermenting solution, uniformly stirring, and fermenting at the temperature of 28-30 DEG C and the speed of 150rpm for 60-72 hours; after fermenting, sterilizing at 115 DEG C for 15 minutes, performing centrifugal separation, concentrating supernatant liquor until the sugar degree is 20-30Bx, and performing spray-drying to obtain the potato protein peptide. The processing method is mild in reaction and relatively easy to control; the potato protein peptide is low in impurity content, free from peculiar smell such as bitter taste and astringent taste, and relatively low in production cost.

Description

The processing method of potato protein peptide
Technical field
The present invention relates to a kind of processing method of protein, specifically a kind of processing method of potato protein peptide.
Background technology
Potato, except containing abundant starch, also contains protein.Potato protein meal is the byproduct producing in farina processing process.The crude protein content of potato protein meal reaches 75% left and right, NFE 13% left and right, and not only protein content is high, and its amino acid composition is quite balanced, and the average digestibility of its essential amino acid reaches 87.5%, is a kind of good protein.
Modern scientific research shows, small-molecular peptides has higher biologically active and nutritive value than the amino acid of equivalent, peptide class and amino acid almost all can be absorbed and used by Epithelium of intestinal villus cell completely, enter into blood, then be transported to each position of organism, protein must just can be absorbed and used after digestion.Peptide class is the free amino acid faster than equivalent by organism infiltration rate, greatly faster than the protein of equivalent.At present, the method that potato protein is converted into protein peptides is mainly to have enzymatic isolation method and acid hydrolyzation.Acid hydrolyzation reaction is violent, and adds strong acid, HTHP, and reflection process and end product are all difficult to control, and can cause a large amount of amino acid whose destruction, and cause environmental pollution; Though enzyme process reaction condition is gentleer, production cost is high, and final products bitter taste is obvious, and mouthfeel is poor.
Summary of the invention
The invention provides a kind of processing method of potato protein peptide.The present invention adopts microbe fermentation method that the protein transduction in potato protein meal is turned to protein peptides.
The object of the present invention is achieved like this: the potato protein meal of take is sized mixing to material concentration 10-15% as raw material, Jia Shui, adjust pH value to 6.5-7.0,121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, press the 8-10% access bacillus subtilis liquid seeds of slurries total amount, stir, under 28 ℃-30 ℃, rotating speed 150rpm condition, fermentation 60-72 hour; Fermentation ends, 115 ℃ of sterilizings 15 minutes, centrifugation, is concentrated into solid content 20-30Bx again by supernatant, the dry potato protein peptide that makes of spraying.
The cultural method of described bacillus subtilis liquid seeds is: take 5g potato protein meal, 4g glucose, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90ml water, stir, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access bacillus subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours.
The present invention be take potato protein meal as raw material, and the method for fermenting by microorganism turns to potato protein peptide by the protein transduction in potato protein meal.Potato protein peptide of the present invention is that molecular weight is the oligopeptide of normal distribution between 500--2500 Dalton, main take the little peptide that formed by 3-6 amino acid as main, it does not need digestion after entering organism, but directly enter small intestine with the form of complete little peptide, be absorbed, and enter human recycle system, bring into play its function, have advantages of that 100% is absorbed by organism and utilizes, without any refuse and excreta.The absorption rate of potato protein peptide will be much larger than potato protein.
Process reaction temperature of the present invention and, than being easier to, control, the impurity content of its final products is low, without the pained peculiar smell that waits, compares with other method of producing potato protein peptide, production cost of the present invention is lower.
Four, specific embodiment
First carry out liquid seeds cultivation, then carry out the preparation of potato protein peptide.
Embodiment one
The cultivation of liquid seeds: take 5g potato protein meal powder, then claim 4g glucose, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, adds 90ml water, stirs, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access bacillus subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be bacillus subtilis liquid seeds.
The preparation of fermentation medium and fermentation process are: take 60g potato protein meal, add 440 grams of water, stir, adjust PH6.5-7.0,121 ℃ of sterilizings 30 minutes, are cooled to 30 ℃, access 45 grams of bacillus subtilis liquid seeds, under 28 ℃-30 ℃, rotating speed 150rpm condition, cultivate 66 hours, after fermentation ends, 115 ℃ of sterilizings 15 minutes, centrifugation, supernatant evaporation and concentration is to 25Bx, the spray-dried potato protein peptide that makes.
Embodiment two
The cultivation of liquid seeds: take 10g potato protein meal powder, then claim 8g glucose, 0.2g potassium dihydrogen phosphate, 0.1g magnesium sulfate, adds 180ml water, stirs, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access bacillus subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours, detect qualified after, be bacillus subtilis liquid seeds.
The preparation of fermentation medium and fermentation process are: take 140g potato protein meal, add 860 grams of water, stir, adjust PH6.5-7.0,121 ℃ of sterilizings 30 minutes, are cooled to 30 ℃, access 90 grams of bacillus subtilis liquid seeds, under 28 ℃-30 ℃, rotating speed 150rpm condition, cultivate 70 hours, after fermentation ends, 115 ℃ of sterilizings 15 minutes, centrifugation, supernatant evaporation and concentration is to 30Bx, the spray-dried potato protein peptide that makes.

Claims (2)

1. the processing method of a potato protein peptide, it is characterized in that: the potato protein meal of take is sized mixing to material concentration 10-15% as raw material, Jia Shui, adjust pH value to 6.5-7.0,121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, the 8-10% access bacillus subtilis liquid seeds by slurries total amount, stirs, under 28 ℃-30 ℃, rotating speed 150rpm condition, fermentation 60-72 hour; Fermentation ends, 115 ℃ of sterilizings 15 minutes, centrifugation, is concentrated into solid content 20-30Bx again by supernatant, the dry potato protein peptide that makes of spraying.
2. the cultural method of bacillus subtilis liquid seeds claimed in claim 1 comprises the following steps: take 5g potato protein meal, 4g glucose, 0.1g potassium dihydrogen phosphate, 0.05g magnesium sulfate, add 90ml water, stir, 121 ℃ of sterilizings 30 minutes, be cooled to 30 ℃, access bacillus subtilis inclined-plane seed, stirs, under 28 ℃-30 ℃, rotating speed 200rpm condition, cultivate 24 hours.
CN201310577075.1A 2013-11-19 2013-11-19 Processing method of potato protein peptide Pending CN103609833A (en)

Priority Applications (1)

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CN201310577075.1A CN103609833A (en) 2013-11-19 2013-11-19 Processing method of potato protein peptide

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Application Number Priority Date Filing Date Title
CN201310577075.1A CN103609833A (en) 2013-11-19 2013-11-19 Processing method of potato protein peptide

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CN103609833A true CN103609833A (en) 2014-03-05

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087643A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Method for preparing potato protein polypeptide
CN104397319A (en) * 2014-12-05 2015-03-11 黑龙江省轻工科学研究院 Method for preparing potato protein bioactive peptide by using microorganism fermentation method
CN105558260A (en) * 2015-12-29 2016-05-11 陕西天宝大豆食品技术研究所 Potato peptide nutrient and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0339049A (en) * 1989-07-07 1991-02-20 Ajinomoto Co Inc Production of low-molecular peptide mixture
CN1579198A (en) * 2003-08-15 2005-02-16 中国农业大学 Active peptide feed additive and its preparation method and use
CN102719505A (en) * 2012-05-14 2012-10-10 梁金钟 Method for preparing vegetable protein small peptide by microoprganism fermentation
CN102823731A (en) * 2012-08-02 2012-12-19 广州市博善生物饲料有限公司 Method for preparing small feed composite peptide

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0339049A (en) * 1989-07-07 1991-02-20 Ajinomoto Co Inc Production of low-molecular peptide mixture
CN1579198A (en) * 2003-08-15 2005-02-16 中国农业大学 Active peptide feed additive and its preparation method and use
CN102719505A (en) * 2012-05-14 2012-10-10 梁金钟 Method for preparing vegetable protein small peptide by microoprganism fermentation
CN102823731A (en) * 2012-08-02 2012-12-19 广州市博善生物饲料有限公司 Method for preparing small feed composite peptide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
翁洋,等: "微生物发酵制备小肽的研究", 《中国畜牧兽医学会动物微生态学分会第四届第九次学术研讨会论文集(上册)》, 31 December 2008 (2008-12-31), pages 212 - 215 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104087643A (en) * 2014-07-29 2014-10-08 哈尔滨伟平科技开发有限公司 Method for preparing potato protein polypeptide
CN104397319A (en) * 2014-12-05 2015-03-11 黑龙江省轻工科学研究院 Method for preparing potato protein bioactive peptide by using microorganism fermentation method
CN105558260A (en) * 2015-12-29 2016-05-11 陕西天宝大豆食品技术研究所 Potato peptide nutrient and preparation method thereof
CN105558260B (en) * 2015-12-29 2019-09-10 陕西天宝大豆食品技术研究所 Potato peptide nutrient food and preparation method thereof

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Application publication date: 20140305