CN103604768A - Portable UV spectrophotometer special for CO poisoning and application method thereof - Google Patents
Portable UV spectrophotometer special for CO poisoning and application method thereof Download PDFInfo
- Publication number
- CN103604768A CN103604768A CN201310629735.6A CN201310629735A CN103604768A CN 103604768 A CN103604768 A CN 103604768A CN 201310629735 A CN201310629735 A CN 201310629735A CN 103604768 A CN103604768 A CN 103604768A
- Authority
- CN
- China
- Prior art keywords
- core waveguide
- flow cell
- poisoning
- liquid core
- light source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a portable UV spectrophotometer special for CO poisoning and an application method thereof. The portable UV spectrophotometer comprises an integrated sampling probe, a liquid-core waveguide flow-through cell, a press pump, a LED light source, a minisize detector, an optical coupler, a control circuit, a cell and the like; the integrated sampling probe, the liquid-core waveguide flow-through cell and the optical coupler are formed by a capillary; the light source is a plug-in LED light source and different wavelength LED can be employed according to demands; the sample is introduced by the press pump, and the manner is simple and reliable; and a matched kit comprises a cleaning solution and a diluent. The application method comprises: firstly cleaning the flow-through cell, then testing a referential solution and a to-be-tested sample solution under different detection wavelength, and finally obtaining the concentration of carboxyhemoglobin in the blood sample by software transition, and displaying on the minisize detector. The beneficial effects comprise that: the UV spectrophotometer is simple and portable, simple in operation, accurate in concentration determination of carbon monoxide in blood, small in sample consumption, flexible in usage and high in spreading value.
Description
Technical field
The present invention relates to a kind of spectrophotometer, specifically the poisoning special portable formula of a kind of CO uv-spectrophotometric instrument and using method thereof.
Background technology
Along with scientific and technological development and social progress, analytical test has become the mankind's one of science and technology activity the most frequently, and social and economic development improves constantly the interdependent degree of analytical and testing instrument.And along with the reach of science, each application improves constantly the requirement of analytical test result real-time, on-the-spot express-analysis has become one of development trend of analysis science.On-the site analysis certainly will require microminiaturization and the portability of analytical instrument, and equipment miniaturization has become an important directions of analytical instrument development.
Carbon monoxide poisoning event is easy to occur in China remote districts winter, constantly perfect along with China's legal construction, particularly punishment is told method the requirement of evidence is improved constantly, for this type of event, blood is sample unique in anthracemia case, necessarily requires to make the mensuration of carbonomonoxide concentration in blood.Blood reveals to be put in air, and in blood, carbonyl hemoglobin is easily replaced and lost by airborne oxygen, makes testing result inaccurate.Therefore, how in event spot fast, the concentration ，Bing Neng basic unit of carbon monoxide promotes the use of and becomes difficult point in Accurate Determining blood.
At present, the judgement of CO degree of intoxication is mainly relied on the mensuration of carbonyl hemoglobin (carbonylhemoglobin, HbCO) content [HbCO] % in body.Concrete grammar comprises isosbestic point method [document 1. Siek T J. Determination of Carboxyhemoglobin in the Presence of Other Blood Hemoglobin Pigments by Visible Spectrophotometry[J]. Journal of Forensic Science, 1984,29:39-54], Simultaneous Equations method [document 2. Rodkey F l et al. Spectrophotometric Measurement of Carboxyhemoglobin and Methemoglobin in Blood[J]. Clinical Chemistry, 1979,25:1388-1393], double wave regular way [document 3. Sakata M et al. Simple Determination of Carboxyhemoglobin by Double Wave length Spectrophotometry of Absorbance and the Comparison with Gas Chromatographic Method[J]. Forensic Science International. 1983,21:187-195], derivative spectrophotometry [document 4. Tan family's an ancient unit of weight equal to 20 or 24 *taels of silvers, Yang Ming. Four order derivative UV spectrophotometry method is measured blood Traffic policemen [J]. Chinese law medical journal, 1988,3:206-209] etc.Wherein derivative spectrophotometry need to be used the instrument that can carry out derivative spectrum mensuration, and application is subject to certain limitation; Isosbestic point method and Simultaneous Equations method are subject to the impact of blood clarity, measure accuracy relatively poor; Double wave regular way need to be carried out mensuration 2 times, although comparatively loaded down with trivial details, the accuracy of measuring is higher.Common uv-spectrophotometric instrument generally can only be measured the carbon monoxide in blood under laboratory condition, instrument is generally undertaken by the technician or the legal medical expert that specialize in physical and chemical inspection, and the most cheap uv-spectrophotometric instrument is generally also wanted nearly ten thousand yuan, operation and daily servicing are relatively loaded down with trivial details, are not suitable for on-the-spot quick test.The import microspectrophotometer of use is mainly Nanodrop ND-1000 in the market, and its price is ten thousand yuan of 8-9, and expensive and complicated operation, is unfavorable for penetration and promotion.
Summary of the invention
The technical problem to be solved in the present invention is: a kind of poisoning special portable formula of CO uv-spectrophotometric instrument that detects principle based on liquid core waveguide is provided.
The present invention solves the technical scheme that its technical matters adopts: the poisoning special portable formula of CO uv-spectrophotometric instrument adopts liquid core waveguide to detect principle, integrative sampling probe, liquid core waveguide flow cell, Pressing pump, LED light source, micro-detector, control circuit, photo-coupler and battery etc., consists of.Integrative sampling probe, liquid core waveguide flow cell and photo-coupler consist of a capillary.Light source is selected plug-in LED light source, can adopt as required different wave length LED.Adopt Pressing pump to introduce sample, simple and reliable.
Test solution enters liquid core waveguide flow cell by Pressing pump through integrative sampling probe, the light of LED light source transmitting enters liquid core waveguide flow cell after filter system becomes monochromatic light, and in flow cell along axis propagation, finally through photo-coupler, by detecting device, received, after conversion processing, obtain test result.
This type of uv-spectrophotometric instrument can be used as the criminal technique equipment that must join and is equipped with to reclamation area criminal technique department of (at county level) public security bureau.
A using method for the poisoning special portable formula of CO uv-spectrophotometric instrument, concrete steps are as follows:
1, the configuration of blood sample: get blood sample 20～50uL, carry out the dilution of 40～100 times with thinning agent;
2, detecting device being detected to wavelength regulation is the first wavelength 420nm, by Pressing pump, cleaning fluid is sucked to liquid core waveguide flow cell, cleans liquid core waveguide flow cell; Then reference solution is sucked in liquid core waveguide flow cell, test the absorbance of reference solution under the first wave band, proofreading and correct absorbance is 0;
3, again clean after liquid core waveguide flow cell, sample solution is sucked in liquid core waveguide flow cell, under the first wave band, test its absorbance;
4, detecting device being detected to wavelength regulation is second wave length 432nm, repeating step 1 and step 2, the absorbance of specimen solution;
5, instrument uses formula to calculate, and obtains the concentration of HbCO in blood sample, and is presented on screen.
The invention has the beneficial effects as follows:
1, highly sensitive.The present invention is based on micro-fluidic liquid core waveguide principle, light source light enters optical fiber to optimize differences in angle, and incident light is propagated along shaft axis of optic fibre in optical fiber, and through outer wall, can be in optical fiber continuous excited sample, large leap ahead light path, and improve the utilization factor of incident light;
2, volume is little, is easy to carry.Utilize LED for light source, its volume is little, quality light, light intensity is higher, integrated light path and Control System Design, and machine volume is little;
3, need sample size few.Using liquid core waveguide optical fiber is liquid core waveguide flow cell, and pond volume is less than 1 μ L, and the required sample size of whole test is few;
4, simple to operate.By circuit and the control program of design specialized, by data acquisition, signal is processed and computing, a series of process implementation robotizations such as process control and result demonstration;
5, applied range.It is light source that liquid core waveguide spectrometer adopts plug-in monochromatic LED, for sample maximum absorption wavelength, measures, and also can realize multi-wavelength detection by changing the LED light source of different wave length.
accompanying drawing explanationbelow in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is schematic diagram of the present invention.
Wherein: 1. LED light source, 2. filter system, 3. integrative sampling probe, 4. liquid core waveguide flow cell, 5. photo-coupler, 6. micro-detector, 7. Pressing pump, 8. battery, 9. control circuit
[0018] embodimentthe invention will be further described in conjunction with specific embodiments now, and following examples are intended to illustrate the present invention rather than limitation of the invention further.
Embodiment: as shown in Figure 1, the poisoning special portable formula of a kind of CO based on micro-fluidic liquid core waveguide principle uv-spectrophotometric instrument, comprise integrative sampling probe (3), liquid core waveguide flow cell (4), Pressing pump (7), LED light source (1), filter system (2), photo-coupler (5), micro-detector (6), control circuit (9) and battery (8), integrative sampling probe (3), liquid core waveguide flow cell (4), the quartz capillary that photo-coupler (5) is applied by 50 μ m ID * 20mm Teflon AF2400 forms, whole instrument is powered and is controlled by control circuit (9) by battery (8), the light that LED light source (1) emits is photosystem (2) after filtration successively, liquid core waveguide flow cell (4), photo-coupler (5), finally by micro-detector (6), received.The effective light path that has obtained approximately 10 mm, its detection volume is 350 nL.
Adopt Teflon AF 2400 kapillaries as flow cell, the refractive index of this Teflon material (1.29) is low compared with water (1.33), so luminous energy is bound in aqueous solution, along capillary tube inner wall, advances and obtain long light path, reach thus the object of highly sensitive micro-consumption.
Get blood sample 20uL, the dilution of carrying out 40 times with thinning agent is mixed with blood sample.Use 420nm and 432nm two cover filter systems, according to abovementioned steps, the absorbance A of working sample under 420nm and two kinds of wavelength of 432nm
420and A
432, the computing formula of the content of carbonyl hemoglobin is [HbCO] %=(1.317A
420-0.988A
432)/(0.844A
420+ 0.982A
432) * 100%, tests concentration [HbCO] % that directly demonstrates HbCO in sample in rear instrument screen.
This apparatus structure is simply easy to use, with low cost, and usable range is wide, is easy to carry out the feature of microminiaturized and portability.
The above-mentioned foundation desirable embodiment of the present invention of take is enlightenment, and by above-mentioned description, relevant staff can, within not departing from the scope of this invention technological thought, carry out various change and modification completely.The technical scope of this invention is not limited to the content on instructions, must determine its technical scope according to claim scope.
Claims (5)
1. the poisoning special portable formula of CO uv-spectrophotometric instrument and a using method thereof, is characterized in that: device comprises LED light source (1), filter system (2), integrative sampling probe (3), liquid core waveguide flow cell (4), photo-coupler (5), micro-detector (6), Pressing pump (7), battery (8) and control circuit (9); Integrative sampling probe (3), liquid core waveguide flow cell (4), photo-coupler (5) consist of a capillary; Whole instrument is powered and is controlled by control circuit (9) by battery (8), the light that LED light source (1) emits is photosystem (2), liquid core waveguide flow cell (4), photo-coupler (5) after filtration successively, finally by micro-detector (6), received, and show on screen by conversion.
2. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: matched reagent box is comprised of cleaning fluid and thinning agent; Cleaning fluid is 0.1-0.2%Na
2cO
3solution (W/W); Thinning agent is for containing 0.1-0.2% Na
2cO
3(W/W), 0.2-0.3%Na
2s
2o
4(W/W) aqueous solution, this solution is also reference solution.
3. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, it is characterized in that: the light that LED light source (1) emits the first wavelength that photosystem (2) obtains after filtration is successively 420nm, and second wave length is 432nm.
4. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: concrete method of testing is:
The configuration of A blood sample: get blood sample 20～50uL, carry out the dilution of 40～100 times with thinning agent;
B concrete operation step:
A, detecting device is detected to wavelength regulation is the first wavelength, by Pressing pump (7), cleaning fluid is sucked to liquid core waveguide flow cell (4), cleans flow cell; Then reference solution is sucked in liquid core waveguide flow cell (4), the absorbance of testing reference solution under the first wave band, proofreading and correct absorbance is 0;
B, again clean after liquid core waveguide flow cell (4), sample solution is sucked in liquid core waveguide flow cell (4), under the first wave band, test its absorbance;
C, detecting device is detected to wavelength regulation is second wave length, repeating step 1 and step 2, the absorbance of specimen solution;
D, instrument are converted to carbonyl hemoglobin concentration in blood by test result, and show on screen.
5. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and user thereof
The poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: described kapillary is the Teflon kapillary of ultraviolet light thoroughly; Micro-detector (6) can be miniature photomultiplier or CCD; Filter system (2) can be optical filter, grating or monochromator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310629735.6A CN103604768A (en) | 2013-11-29 | 2013-11-29 | Portable UV spectrophotometer special for CO poisoning and application method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310629735.6A CN103604768A (en) | 2013-11-29 | 2013-11-29 | Portable UV spectrophotometer special for CO poisoning and application method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103604768A true CN103604768A (en) | 2014-02-26 |
Family
ID=50123013
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310629735.6A Pending CN103604768A (en) | 2013-11-29 | 2013-11-29 | Portable UV spectrophotometer special for CO poisoning and application method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103604768A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105092492A (en) * | 2014-05-06 | 2015-11-25 | 黄辉 | Light guide capillary-based photometric analyzer and detection method thereof |
| CN109709051A (en) * | 2018-12-26 | 2019-05-03 | 桂林电子科技大学 | A kind of online spectrophotometric detector of nitrite nitrogen based on integrated molding flow cell |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5302348A (en) * | 1992-12-10 | 1994-04-12 | Itc Corporation | Blood coagulation time test apparatus and method |
| US6084661A (en) * | 1998-05-07 | 2000-07-04 | Worcestor Polytechnic Institute | Optical method and apparatus for measuring carbon monoxide in non-hemolyzed blood |
| CN1610827A (en) * | 2001-12-28 | 2005-04-27 | 海莫库公司 | Method for quantitative hemoglobin determination in undiluted unhemolyzed whole blood |
| CN101319993A (en) * | 2007-05-03 | 2008-12-10 | 霍夫曼-拉罗奇有限公司 | Oximeter |
| CN101806726A (en) * | 2010-03-29 | 2010-08-18 | 浙江大学 | Double-wavelength absorbance detection device for analyzing trace liquid |
| CN203310744U (en) * | 2013-06-25 | 2013-11-27 | 成都君亚科技有限公司 | Liquid core waveguide food detector |
-
2013
- 2013-11-29 CN CN201310629735.6A patent/CN103604768A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5302348A (en) * | 1992-12-10 | 1994-04-12 | Itc Corporation | Blood coagulation time test apparatus and method |
| US6084661A (en) * | 1998-05-07 | 2000-07-04 | Worcestor Polytechnic Institute | Optical method and apparatus for measuring carbon monoxide in non-hemolyzed blood |
| CN1610827A (en) * | 2001-12-28 | 2005-04-27 | 海莫库公司 | Method for quantitative hemoglobin determination in undiluted unhemolyzed whole blood |
| CN101319993A (en) * | 2007-05-03 | 2008-12-10 | 霍夫曼-拉罗奇有限公司 | Oximeter |
| CN101806726A (en) * | 2010-03-29 | 2010-08-18 | 浙江大学 | Double-wavelength absorbance detection device for analyzing trace liquid |
| CN203310744U (en) * | 2013-06-25 | 2013-11-27 | 成都君亚科技有限公司 | Liquid core waveguide food detector |
Non-Patent Citations (2)
| Title |
|---|
| 张菁菁等: "双波长吸光度比值法检测碳氧血红蛋白", 《临床检验杂志》, vol. 26, no. 1, 31 January 2008 (2008-01-31), pages 61 - 62 * |
| 杨娅等: "可见分光光度法快速检测碳氧血红蛋白饱和度", 《现代科学仪器》, no. 6, 31 December 2008 (2008-12-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105092492A (en) * | 2014-05-06 | 2015-11-25 | 黄辉 | Light guide capillary-based photometric analyzer and detection method thereof |
| CN109709051A (en) * | 2018-12-26 | 2019-05-03 | 桂林电子科技大学 | A kind of online spectrophotometric detector of nitrite nitrogen based on integrated molding flow cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102519897B (en) | Water quality COD detection method and apparatus based on LED multi-feature wavelength | |
| JP2008523413A5 (en) | ||
| KR101684407B1 (en) | Water pollution measurement system using optical sensor and water pollution measurement apparatus | |
| CN102809544B (en) | Total plumbous online automatic monitor | |
| CN102156100A (en) | Multispectral-based multipoint sampling multiparameter water quality on-line analytical system | |
| CN102564983B (en) | Optical detection device and method for concentration of components in turbid medium based on limited range wavelength method | |
| CN202002881U (en) | Multispectral-based multipoint-sampling multi-parameter water quality online analysis system | |
| CN202916200U (en) | Medical fluorescent quantitation analysis meter | |
| CN208255048U (en) | Fibre-optical probe and water quality detecting device | |
| CN103604768A (en) | Portable UV spectrophotometer special for CO poisoning and application method thereof | |
| CN202837188U (en) | Portable optical fiber surface enhanced Raman scattering spectrometer | |
| CN202092944U (en) | Device for detecting ultramicro sample | |
| CN208537406U (en) | One kind being based on microflow control technique original position ammonia nitrogen on-line computing model | |
| CN101183070A (en) | Bar-shape spectrophotometric device | |
| Kröckel et al. | Multiscale spectroscopy using a monolithic liquid core waveguide with laterally attached fiber ports | |
| CN101251534B (en) | Biochemistry and medical hollow core prague optical fiber measurement analysis system | |
| RU2555507C1 (en) | Analyser of composition of expired air | |
| CN103398966A (en) | Method for detecting TMC concentration in organic solution by using spectrometer | |
| CN208255047U (en) | Fibre-optical probe and water quality detecting device | |
| CN202404052U (en) | Liquid detection chip | |
| CN203479695U (en) | Instrument for on-line measurement of concentration of TMC (trimesoyl chloride) in organic solution | |
| CN104949928A (en) | Novel on-line detection photometer | |
| CN112525867A (en) | Fluorescent compound micro-flow detector based on tapered single-mode fiber | |
| CN205246529U (en) | Hexavalent chromium survey device in water sample based on little reaction system flows | |
| CN206114517U (en) | Infrared oil smoke monitoring system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140226 |