CN103583512B - Plant specimen is preserved gel and preparation method thereof and purposes - Google Patents
Plant specimen is preserved gel and preparation method thereof and purposes Download PDFInfo
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- CN103583512B CN103583512B CN201310567720.1A CN201310567720A CN103583512B CN 103583512 B CN103583512 B CN 103583512B CN 201310567720 A CN201310567720 A CN 201310567720A CN 103583512 B CN103583512 B CN 103583512B
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- plant
- gel
- specimen
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- 241000196324 Embryophyta Species 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000001879 gelation Methods 0.000 title abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 11
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000011780 sodium chloride Substances 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 23
- 201000010099 disease Diseases 0.000 claims description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 8
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 5
- 241001237160 Kallima inachus Species 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 2
- 238000004321 preservation Methods 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 abstract description 5
- 239000005418 vegetable material Substances 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 2
- -1 acrylamide compound Chemical class 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 241000026010 Dendrobium candidum Species 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 241001523681 Dendrobium Species 0.000 description 3
- 239000003086 colorant Substances 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 239000002932 luster Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229940099259 vaseline Drugs 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses plant specimen and preserve gel and preparation method thereof and purposes, belong to environmental friendly material field, the program of fixedly killing livestock of vegetable material has been simplified in its operating process, operate more easy, when plant specimen is preserved, gel used is the acrylamide compound that is 20%-25% by mass ratio, the N of 2%-3%, and the solution that the PH that N-methylene-bisacrylamide, 15%-20% dodecyl sodium sulfate, 0.9% sodium chloride, 0.5%-1.0% tetramethylethylenediamine and 50.1%-61.6% water form is 8.5-9.0 is made. PH 8.5-9.0 has effectively suppressed growing of vegetative bacteria and mould, has solved problem of environmental pollution when sample is preserved storage. The preservation of monochromatic plant, secondary color plant and the plant total preparations such as it is green, yellow, red, white that the present invention is applicable to, for new approach has been opened up in the making of plant specimen.
Description
Technical field
The present invention relates to a kind of Slide processing, is that a kind of plant specimen is preserved gel specificallyPreparation method.
Background technology
The primary colors original state sample of plant not only has very important effect in teaching and scientific research, andAlso there is important value at aspects such as sample display, displaying and artistic ornamentals, due to plantThe complex chemical composition containing, its chemical change rule is difficult to grasp, and gives primary colour plant sampleMaking brought very large difficulty.
At present, traditional plant primary colour specimen is made and is had following subject matter:
One, preserve liquid and adopt some toxic reagents as formalin, sulfurous acid etc. more, these thingsMatter is unstable, volatile, harmful;
Two, adopt respectively different fixers and preserve liquid preserve different colours vegetable material asGreen plants sample fixer and preservation liquid, red plant specimen fixer and preservation liquid, purplePlant specimen fixer and preservation liquid, white plant specimen fixer and preservation liquid and and yellowPlant specimen fixer and preservation liquid etc.;
Three, these traditional plant primary colour specimen preparation methods, need to prepare numerous fixersWith preservation liquid, workload is very large, also usually can and preserve liquid preparation surplus because of fixer simultaneouslyBring very large waste;
Four, even more important a bit, the branches and leaves of any plant, flower, fruit color are oftenNot consistent, cannot carry out entirety to it by traditional method and preserve on the whole, traditionalPlant primary colour specimen method for production application surface is narrow, unrealistic and expense is higher.
Summary of the invention
The present invention is directed to the deficiency of the problems referred to above, provide a kind of plant specimen to preserve gel making sideMethod, its method is simple, efficient, stable, plant specimen can be stored in for a long time without poison ringIn the preservation gel of protecting, and be applicable to the making of the plant specimen of multiple color.
The present invention for addressing the above problem adopted technical scheme is:
A kind of plant specimen is preserved gel preparation method, its needed raw material component and quality proportioning asUnder:
Its manufacturing process is:
By acrylamide, N, N-methylene-bisacrylamide, dodecyl sodium sulfate, chlorinationThe pH that sodium and water form is that 8.5-9.0 solution prepares and puts into preprepared sampleIn bottle, then add wherein 0.5%-1.0% tetramethylethylenediamine, leaving standstill five minutes solution willForm gel.
The simultaneously claimed plant specimen that utilizes this method and make of the present invention is preserved gel.
A preparation method for plant specimen, its concrete steps are:
1) collection of plant specimen material and pretreatment
Collection is grown normally, is made sample material without the plant of disease and pest, cuts off disease dead leaf, incompletenessInfull flower and too much branches and leaves, requirement retains the integrality of plant, retains its Classification and IdentificationFeature, after the fresh plant sample material running water after gathering is cleaned, is used filter paper by waterPoint blot stand-by;
2) plant specimen material fixing in gel
By the fresh plant sample material obtaining through above-mentioned steps put into by mass ratio be 20%-25% acrylamide, the N of 2%-3%, N-methylene-bisacrylamide, 15%-20% tenThe pH that the water of dialkyl group sodium sulfonate, 0.9% sodium chloride and surplus forms is 8.5-9.0Solution in, then add wherein 0.5%-1.0% tetramethylethylenediamine, leave standstill five minutes moltenLiquid will form gel.
The invention has the beneficial effects as follows: solve problem of environmental pollution when sample is preserved storage,PH 8.5-9.0 has effectively suppressed growing of vegetative bacteria and mould, its operating process letterChange the fixed routine of killing livestock of vegetable material, operated more easyly, that the present invention is applicable to is green, yellow,The persistence of monochromatic plant, secondary color plant and the plant total preparation such as red, white is plantNew approach has been opened up in the making of sample.
Brief description of the drawings
The present invention has accompanying drawing 2 width, for obtained the photo of sample according to embodiment 1. ItsIn:
Fig. 1 is the positive elevation view of dendrobium candidum sample;
Fig. 2 is the handstand view of dendrobium candidum sample.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but these embodiment do not limitProtection scope of the present invention.
Embodiment 1
A kind of economic environment protecting plant sample of the present invention is preserved gel preparation method, for dendrobium candidumThe concrete steps that the gel of sample is preserved are:
Step 1) collection and the pretreatment of plant specimen material
Selection growth is normal, cutting off disease dead leaf without the dendrobium candidum plant specimen material of disease and pest willAsk the integrality that retains plant, retain its Classification and Identification feature, by the fresh plant mark after gatheringThis uses filter paper moisture to be blotted stand-by after cleaning with running water.
Step 2) plant specimen material fixing in gel
By above-mentioned steps 1) in after running water is cleaned, use filter paper to treat after moisture is blottedWith vegetable material put into by mass ratio the acrylamide that is 20%, 2% N, N-di-2-ethylhexylphosphine oxideAcrylamide, 20% dodecyl sodium sulfate, 0.9% sodium chloride and the water institute group of surplusIn the solution that the pH becoming is 8.5, then add wherein 0.5% tetramethylethylenediamine, leave standstill fiveMinute solution will form gel. Finally with vaseline by the specimen bottle lid place reality of obturaging, be put into dryPersistence is carried out in dry, shady and cool place.
Respectively 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation,Sample was observed in 36 months, found state, color and luster, the shape of dendrobium candidum plant specimenShape is all without any change.
Latter 36th month of sample preparation, the taking-up of dendrobium candidum plant specimen is rinsed to rear observation,Find that dendrobium candidum plant, all without any change, is placed in normal indoor environment 15 afterwardsAfter individual month, observe and find that dendrobium candidum plant rots.
Acrylamide compound, N, N-methylene-bisacrylamide, dodecyl sodium sulfate shapeBecome gel to there is solid shape and transparent effect to plant; Tetramethylethylenediamine has accelerates itThe aqueous solution form gel effect; The alkaline solution that described solution is 8.5-9.0 by pH,There is sterilization, corrosion-resistant effect; 0.9% sodium chloride has the infiltration pressing that keeps plant cellThe effect of weighing apparatus.
Result shows, adopts plant primary colors gel Slide processing of the present invention to preserve dendrobium candidumSeedling and callus, can preserve for a long time.
Embodiment 2
A kind of economic environment protecting plant sample of the present invention is preserved gel preparation method, for dendrobium candidumThe concrete steps that the gel of sample is preserved are:
Step 1) collection and the pretreatment of plant specimen material
Selection growth is normal, cutting off disease dead leaf without the dendrobium candidum plant specimen material of disease and pest willAsk the integrality that retains plant, retain its Classification and Identification feature, by the fresh plant mark after gatheringThis uses filter paper moisture to be blotted stand-by after cleaning with running water.
Step 2) plant specimen material fixing in gel
By above-mentioned steps 1) in after running water is cleaned, use filter paper to treat after moisture is blottedWith vegetable material put into by mass ratio the acrylamide that is 25%, 3% N, N-di-2-ethylhexylphosphine oxideAcrylamide, 15% dodecyl sodium sulfate, 0.9% sodium chloride and the water institute group of surplusIn the solution that the pH becoming is 9, then add wherein 1.0% tetramethylethylenediamine, leave standstill five pointsClock solution will form gel. Finally with vaseline by the specimen bottle lid place reality of obturaging, be put into dry,Persistence is carried out in shady and cool place.
Respectively 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation,Sample was observed in 36 months, found state, color and luster, the shape of dendrobium candidum plant specimenShape is all without any change.
Claims (1)
1. a preparation method for plant specimen, is characterized in that, concrete steps are:
1) collection of plant specimen material and pretreatment
Collection is grown normally, is made sample material without the plant of disease and pest, cuts off disease dead leaf, incompletenessInfull flower and too much branches and leaves, requirement retains the integrality of plant, retains its Classification and IdentificationFeature, after the fresh plant sample material running water after gathering is cleaned, is used filter paper by waterPoint blot stand-by;
2) plant specimen material fixing in gel
By the fresh plant sample material obtaining through above-mentioned steps put into by mass ratio be 20%-25% acrylamide, the N of 2%-3%, N-methylene-bisacrylamide, 15%-20% tenThe pH that dialkyl group sodium sulfonate, 0.9% sodium chloride and 50.1%-61.6% water form isIn 8.5-9.0 solution, then add wherein 0.5%-1.0% tetramethylethylenediamine, leave standstillWithin five minutes, solution will form gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567720.1A CN103583512B (en) | 2013-11-14 | 2013-11-14 | Plant specimen is preserved gel and preparation method thereof and purposes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567720.1A CN103583512B (en) | 2013-11-14 | 2013-11-14 | Plant specimen is preserved gel and preparation method thereof and purposes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103583512A CN103583512A (en) | 2014-02-19 |
| CN103583512B true CN103583512B (en) | 2016-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310567720.1A Expired - Fee Related CN103583512B (en) | 2013-11-14 | 2013-11-14 | Plant specimen is preserved gel and preparation method thereof and purposes |
Country Status (1)
| Country | Link |
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| CN (1) | CN103583512B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912482B (en) * | 2017-03-07 | 2020-05-12 | 山东中医药大学 | A kind of preparation method of cattail plant specimen |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1048887A (en) * | 1989-07-21 | 1991-01-30 | 中国人民解放军第一医院 | The preparation of Gelled biological fixatives |
| WO1996004943A1 (en) * | 1994-08-10 | 1996-02-22 | Maloe Vnedrencheskoe Predpriyatie 'interfall' | Biologically compatible hydrogel |
| CN1320645A (en) * | 2000-04-21 | 2001-11-07 | 郑永碧 | Process for preparing, purifying and testing medical hydrogel of polyacrylamide |
| CN1391106A (en) * | 2002-06-25 | 2003-01-15 | 上海晶泰生物技术有限公司 | Pre-prepared colloid |
| CN102835390A (en) * | 2012-08-23 | 2012-12-26 | 黑龙江东方学院 | Preparation method of detachable plant three-dimensional specimen |
| CN103004757A (en) * | 2012-12-25 | 2013-04-03 | 山东大学 | Porous temperature-sensitive hydrogel slow release formulation and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3638709A (en) * | 1969-12-11 | 1972-02-01 | Dow Chemical Co | Method of suspending immobilized biological specimens in a transparent gel in a transparent container |
-
2013
- 2013-11-14 CN CN201310567720.1A patent/CN103583512B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1048887A (en) * | 1989-07-21 | 1991-01-30 | 中国人民解放军第一医院 | The preparation of Gelled biological fixatives |
| WO1996004943A1 (en) * | 1994-08-10 | 1996-02-22 | Maloe Vnedrencheskoe Predpriyatie 'interfall' | Biologically compatible hydrogel |
| CN1320645A (en) * | 2000-04-21 | 2001-11-07 | 郑永碧 | Process for preparing, purifying and testing medical hydrogel of polyacrylamide |
| CN1391106A (en) * | 2002-06-25 | 2003-01-15 | 上海晶泰生物技术有限公司 | Pre-prepared colloid |
| CN102835390A (en) * | 2012-08-23 | 2012-12-26 | 黑龙江东方学院 | Preparation method of detachable plant three-dimensional specimen |
| CN103004757A (en) * | 2012-12-25 | 2013-04-03 | 山东大学 | Porous temperature-sensitive hydrogel slow release formulation and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| SDS电泳技术的实验考虑及最新进展;郭尧君;《生物化学与生物物理进展》;19910302;第18卷(第1期);第33页右栏 * |
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| CN103583512A (en) | 2014-02-19 |
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Granted publication date: 20160511 Termination date: 20171114 |