CN103566384A - Deactivating method of vitamin C one-step fermentation broth - Google Patents
Deactivating method of vitamin C one-step fermentation broth Download PDFInfo
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- CN103566384A CN103566384A CN201310470125.6A CN201310470125A CN103566384A CN 103566384 A CN103566384 A CN 103566384A CN 201310470125 A CN201310470125 A CN 201310470125A CN 103566384 A CN103566384 A CN 103566384A
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- step fermentation
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- sorbose
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- 238000000855 fermentation Methods 0.000 title claims abstract description 77
- 230000004151 fermentation Effects 0.000 title claims abstract description 77
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 title claims abstract description 46
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 229930003268 Vitamin C Natural products 0.000 title claims abstract description 21
- 235000019154 vitamin C Nutrition 0.000 title claims abstract description 21
- 239000011718 vitamin C Substances 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 239000002184 metal Substances 0.000 claims abstract description 16
- 238000002679 ablation Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 abstract description 21
- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 abstract description 15
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract 3
- 230000002458 infectious effect Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 description 12
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 9
- 244000173166 Pyrus ussuriensis Species 0.000 description 9
- 239000006052 feed supplement Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 8
- 238000009413 insulation Methods 0.000 description 5
- 239000011229 interlayer Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 230000009849 deactivation Effects 0.000 description 4
- 239000000498 cooling water Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008676 import Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241000589220 Acetobacter Species 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000011169 microbiological contamination Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- GDSOZVZXVXTJMI-SNAWJCMRSA-N (e)-1-methylbut-1-ene-1,2,4-tricarboxylic acid Chemical compound OC(=O)C(/C)=C(C(O)=O)\CCC(O)=O GDSOZVZXVXTJMI-SNAWJCMRSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- RGHNJXZEOKUKBD-QTBDOELSSA-N L-gulonic acid Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O RGHNJXZEOKUKBD-QTBDOELSSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- OESHPIGALOBJLM-REOHCLBHSA-N dehydroascorbate Chemical compound OC[C@H](O)[C-]1OC(=O)C(=O)C1=O OESHPIGALOBJLM-REOHCLBHSA-N 0.000 description 1
- 235000020960 dehydroascorbic acid Nutrition 0.000 description 1
- 239000011615 dehydroascorbic acid Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000000852 hydrogen donor Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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Abstract
The invention relates to a deactivating method of vitamin C one-step fermentation broth. The method is characterized in that the vitamin C one-step fermentation broth is filtered by two levels of liquid filters, wherein a filter core of a first-level liquid filter is a metal membrane with the precision of 0.4 to 0.8 micrometer, and a filter core of a second-level liquid filter is a metal membrane with the precision of 0.1 to 0.3 micrometer. The sorbose in one-step fermentation broth is processed by utilizing two levels of metal-membrane liquid filters, so that the producing bacteria and other infectious microbe in the sorbose liquid of the one-step fermentation broth can be effectively removed, the bacterial eliminating rate is high, the sorbose liquid and nutrients are well reserved, the quality is stable, the sorbose-to-ketogulonigenium conversion rate is increased, and a purpose of saving the energy and reducing the production cost can be reached.
Description
Technical field
The invention belongs to technical field of biological fermentation, particularly relate to a kind of ablation method of vitamin C one-step fermentation liquid.
Background technology
Vitamin C, have another name called ascorbic acid, it is the catalyst in cellular oxidation-reduction reaction, it becomes dehydroascorbate after discharging two hydrogen atoms, while having hydrogen donor to exist, hydroascorbic acid can be accepted two hydrogen atoms and accept to become ascorbic acid, participates in body metabolism, increases body to the resistance infecting.Be used for preventing bad hematic acid and opposing infectious disease, promote wound and union of fracture, and as ancillary drug treatment.
In prior art, vitamin C adopts two-step fermentation preparation:
The first step is to take sorbitol as raw material, and the acetobacter melarogenum of take is converted into sorbitol the process of sorbose as producing bacterium, and its main processes as shown in Figure 1.
Second step is that to take the L-sorbose of first step fermentation gained be raw material, the false pseudomonas bacillus of one strain oxidizing glucose acidfast bacilli (" little bacterium ") and a strain (" large bacterium "), the hybrid bacterial strain fermentation that two kinds of bacterium of size combine naturally generates KGA, transform and refining obtain vitamin C, main processes as shown in Figure 2.
In above-mentioned two-step fermentation process, Pyrusussuriensis sugar liquid, as the main feed supplement of two-step fermentation, is the key intermediate species in two-step fermentation process, and the difference of its quality, directly has influence on whole fermentation level and fermentation rate.At present, for the processing of Pyrusussuriensis sugar liquid, normally before two-step fermentation is used sorbose, it is carried out to deactivation, by the heating and thermal insulation effect of wet-hot steam, remove a step and produce bacterium, thereby obtain relatively aseptic feed supplement Pyrusussuriensis sugar liquid.There are the following problems for this processing mode: 1) wet-hot steam heating and thermal insulation easily destroys the generation of the nutritional labeling in sorbose and one-step fermentation liquid, reduced the quality of Pyrusussuriensis sugar liquid, and then reduce the fermentation rate of vc two-step fermentation; 2) steam and cooling-water consumption are large, have greatly increased production cost; 3) can not be by the black acetobacter complete inactivation in Pyrusussuriensis sugar liquid, especially can not remove miscellaneous bacteria contained in the one-step fermentation liquid (situation that contains miscellaneous bacteria, generally that sorbose content is criticized without the one-step fermentation microbiological contamination tank obviously declining), cause vc bis-step gulonic acid ferment effects undesirable.
Summary of the invention
Object of the present invention is just to overcome the defect of prior art, provides a kind of nutrient substance of avoiding in Pyrusussuriensis sugar liquid destroyed, reduces miscellaneous bacteria quantity in two-step fermentation, improves fermentation rate, the ablation method of the vitamin C one-step fermentation liquid reducing production costs.
The technical scheme taked is for achieving the above object:
A kind of ablation method of vitamin C one-step fermentation liquid, it is characterized in that: vitamin C one-step fermentation liquid is passed through to two-stage liquid filter, wherein the filter element of first order liquid filter is that precision is the metal film of 0.4～0.8 μ m, and the filter element of second level liquid filter is that precision is 0.1～0.3 μ m metal film.
Described vitamin C one-step fermentation liquid is 130～160L/h/m by the speed of two-stage liquid filter
2.
The present invention uses two-stage liquid filter sequentially to connect, and (liquid filter that precision is large is coarse filtration filter, the filter that precision is little is fine straining filter) method of carrying out filtration sterilization substitutes the deactivation mode of wet-hot steam insulation degerming, 1) utilize the metal film of 0.5-0.8 μ m effectively to stop in one-step fermentation liquid that impurity and part produce bacterium, the metal film of 0.1-0.3 μ m effectively stops passing through of each bacterioid (0.3-10 μ m), not only remove the generation bacterium in one-step fermentation liquid, and remove in one-step fermentation liquid may with other miscellaneous bacteria, make bacteria-eliminating efficacy more complete compared with steam deactivation, can guarantee to reach 100% degerming rate, 2) in whole inactivation treatment process, without high-temperature process, Pyrusussuriensis sugar liquid and nutrient substance are retained intact, quality is more stable, thereby improves the fermentation rate of vc two-step fermentation and the fermentation level of 2-KLG, 3) adopt membrane filtration pattern degerming completely, thereby reduced to greatest extent the consumption of steam and cooling water, saving heat energy and water resource can reach more than 60%, have reached energy-saving and cost-reducing effect, 4) metal film filter element is easy to clean, and can repeatedly clean, and filter element is longer service life, 5) filtration step link is less, and easy operating has effectively reduced microbiological contamination link.
To sum up, the mode that use two-stage metal film liquid filter of the present invention is processed one-step fermentation liquid, this kind not only reduced the destruction of the nutrition of one-step fermentation liquid, and significantly improved the aseptic rate of Pyrusussuriensis sugar liquid, improved fermentation level; Obviously reduced the use amount of steam and cooling water simultaneously.
accompanying drawing explanation
Fig. 1 is the process chart that vitamin C adopts the first step in two-step fermentation; Fig. 2 is the process chart that vitamin C adopts second step in two-step fermentation
The specific embodiment
Comparative example
When one-step fermentation tank ferments to terminal, open admission valve, interlayer passes into wet-hot steam; Open interlayer draining (vapour), discharge condensed water.The basic emptying of 15min left and right intermediate water, control throttle flow, make the interior temperature stabilization of tank at 80 ℃ ± 1 ℃, insulation 30min, after insulation finishes, closes interlayer inlet valve, open interlayer water intaking valve, make its interlayer pass into condensed water cooling, by tank temperature drop to 35 ℃ left and right, the Pyrusussuriensis sugar liquid after deactivation is squeezed into feed supplement tank by the pipeline of sterilizing standby.The average fermentation conversion ratio that uses the two-step fermentation tank of this batch of sorbose is 85%, the horizontal 108mg/ml of 2-KLG average fermentation.
Embodiment 1
1) fermentation vitamin C one-step fermentation tank is to terminal stirred and is down to 100rpm, it is mixed.
2) series connection two-stage can sterilized liquid filter (first order cartridge is that precision is the metal film liquid filter core of 0.6 μ m, and second level cartridge is 0.2 μ m metal film liquid filter core), and its import is connected with one-step fermentation tank sampling pipe.
3) use wet-hot steam together with one-step fermentation tank associated conduit, to carry out sterilizing, sterilising conditions to liquid filter: 121 ℃, 0.1Mpa, 20 minutes.
4), after filter sterilised finishes, be naturally cooled to below 100 ℃.
5) open one-step fermentation tank associated conduit, utilize fermentation tank and feed supplement tank tank internal pressure differences (its pressure differential is controlled between 0.05--0.06mpa), multiple tank one-step fermentation liquid can be filtered by two-stage series connection liquid filter continuously, proceed in feed supplement tank standby.
6) the liquid filter rate of filtration is 160L/h/m
2.The sorbose that the outlet of inherent filtration device takes a morsel after filtering under aseptic condition is cultivated on aseptic complex medium.Cultivate 72h, detect without miscellaneous bacteria (comprising that a step produces bacterium and other miscellaneous bacteria).The average fermentation conversion ratio that uses the two-step fermentation tank of this batch of sorbose is 94%.The horizontal 123mg/ml of 2-KLG average fermentation.
Embodiment 2
1) fermentation vitamin C one-step fermentation tank is to terminal stirred and is down to 100rpm, it is mixed.
2) series connection two-stage can sterilized liquid filter (first order cartridge is that precision is the metal film liquid filter core of 0.7 μ m, and second level cartridge is 0.3 μ m metal film liquid filter core), and its import is connected with one-step fermentation tank sampling pipe.
3) use wet-hot steam together with one-step fermentation tank associated conduit, to carry out sterilizing, sterilising conditions to liquid filter: 121 ℃, 0.1Mpa, 20 minutes.
4), after filter sterilised finishes, be naturally cooled to below 100 ℃.
5) open one-step fermentation tank associated conduit, utilize fermentation tank and feed supplement tank tank internal pressure differences (its pressure differential is controlled between 0.05--0.06mpa), multiple tank one-step fermentation liquid can be filtered by two-stage series connection liquid filter continuously, proceed in feed supplement tank standby.
6) the liquid filter rate of filtration is 140L/h/m
2.The sorbose that the outlet of inherent filtration device takes a morsel after filtering under aseptic condition is cultivated on aseptic complex medium.Cultivate 72h, detect without miscellaneous bacteria (comprising that a step produces bacterium and other miscellaneous bacteria).The average fermentation conversion ratio that uses the two-step fermentation tank of this batch of sorbose is 96%, the horizontal 120mg/ml of 2-KLG average fermentation.
Embodiment 3
1) fermentation vitamin C one-step fermentation tank is to terminal stirred and is down to 100rpm, it is mixed.
2) series connection two-stage can sterilized liquid filter (first order cartridge is that precision is the metal film liquid filter core of 0.8 μ m, and second level cartridge is 0.1 μ m metal film liquid filter core), and its import is connected with one-step fermentation tank sampling pipe.
3) use wet-hot steam together with one-step fermentation tank associated conduit, to carry out sterilizing, sterilising conditions to liquid filter: 121 ℃, 0.1Mpa, 20 minutes.
4), after filter sterilised finishes, be naturally cooled to below 100 ℃.
5) open one-step fermentation tank associated conduit, utilize fermentation tank and feed supplement tank tank internal pressure differences (its pressure differential is controlled between 0.05--0.06mpa), multiple tank one-step fermentation liquid can be filtered by two-stage series connection liquid filter continuously, proceed in feed supplement tank standby.
6) the liquid filter rate of filtration is 130L/h/m
2.The sorbose that the outlet of inherent filtration device takes a morsel after filtering under aseptic condition is cultivated on aseptic complex medium.Cultivate 72h, detect without miscellaneous bacteria (comprising that a step produces bacterium and other miscellaneous bacteria).The average fermentation conversion ratio that uses the two-step fermentation tank of this batch of sorbose is 93%, the horizontal 124.5mg/ml of 2-KLG average fermentation.
Claims (2)
1. the ablation method of a vitamin C one-step fermentation liquid, it is characterized in that: vitamin C one-step fermentation liquid is passed through to two-stage liquid filter, wherein the filter element of first order liquid filter is that precision is the metal film of 0.4～0.8 μ m, and the filter element of second level liquid filter is that precision is 0.1～0.3 μ m metal film.
2. according to the ablation method of vitamin C one-step fermentation liquid claimed in claim 1, it is characterized in that: described vitamin C one-step fermentation liquid is 130～160 L/h/m2 by the speed of two-stage liquid filter.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701535A (en) * | 2017-01-19 | 2017-05-24 | 福建凯立生物制品有限公司 | Zhongshengmycin fermentation continuous feeding production device and production method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101525645A (en) * | 2009-04-10 | 2009-09-09 | 河北科技大学 | Membrane-sterilization method for sorbose fermentation medium by using ceramic microfiltration membranes |
| CN103290071A (en) * | 2013-06-09 | 2013-09-11 | 山东天力药业有限公司 | Method for preparing 2-keto-L-gulonic acid |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101525645A (en) * | 2009-04-10 | 2009-09-09 | 河北科技大学 | Membrane-sterilization method for sorbose fermentation medium by using ceramic microfiltration membranes |
| CN103290071A (en) * | 2013-06-09 | 2013-09-11 | 山东天力药业有限公司 | Method for preparing 2-keto-L-gulonic acid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701535A (en) * | 2017-01-19 | 2017-05-24 | 福建凯立生物制品有限公司 | Zhongshengmycin fermentation continuous feeding production device and production method thereof |
| CN106701535B (en) * | 2017-01-19 | 2019-04-09 | 福建凯立生物制品有限公司 | A kind of mesobiotics fermentation continuous feeding production device and production method thereof |
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Application publication date: 20140212 |