CN103505496A - Blood lipid reducing composition containing rosewood and preparation method of composition - Google Patents
Blood lipid reducing composition containing rosewood and preparation method of composition Download PDFInfo
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- CN103505496A CN103505496A CN201210217256.9A CN201210217256A CN103505496A CN 103505496 A CN103505496 A CN 103505496A CN 201210217256 A CN201210217256 A CN 201210217256A CN 103505496 A CN103505496 A CN 103505496A
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a blood lipid reducing composition containing rosewood. The composition comprises the following components by weight percent: 8-87% of ginkgo leaves, 2-79% of safflower and 10-27% of rosewood. The invention also discloses a preparation method of the traditional Chinese medicine composition. The preparation method comprises the steps of extracting active ingredients and preparing the finished product according to the formula. The traditional Chinese medicine composition makes the best of the treatment effects of active pharmaceutical ingredients on cardiovascular diseases, adopts scientific matching, generates synergistic effects, has better curative effects and more controllable quality than traditional ginkgo leaf extract preparations and is suitable for industrial production.
Description
Technical field
The Chinese medicine composition and the preparation method that the present invention relates to a kind of Cardiovarscular, belong to the field of Chinese medicines.
Background technology
Along with improving constantly of people's living standard, the change of dietary structure, aged tendency of population becomes clear day by day, and the sickness rate of coronary heart disease is day by day soaring.Coronary heart disease is because coronary atherosclerosis causes luminal stenosis, causes blood supply of cardiac muscle oxygen supply obstacle and the heart disease that causes, and " world killer " is known as.With regard to the whole world, since half a century, coronary heart disease has become the underlying cause of death of one of the disease that threat human health the is the most serious ，Shi U.S. and some industrialized country.According to World Health Organization's statistics, in the total death toll of the U.S., have 24.7% to die from coronary heart disease, Northern Ireland coronary heart disease case fatality rate occupies first place in the world, and is 5,36/,100,000; Japan is minimum, is 41,/10 ten thousand.In recent years, the M & M of China's coronary heart disease continues soaring, has every year at present 700,000 people of surpassing to die from coronary heart disease, is the modal a kind of cardiovascular diseases of middle-aged and elderly people, has leapt to as first of internal disease.Visible coronary heart disease has become global public hazards, and American wins cardiopathia for " pestilence in epoch ".
Hyperlipidemia is the risk factor of apoplexy, coronary heart disease, myocardial infarction, sudden death.In addition, hyperlipidemia is also to promote hypertension, an important risk factor of impaired glucose tolerance, diabetes.Hyperlipidemia also can cause fatty liver, liver cirrhosis, cholelithiasis, pancreatitis, retinal hemorrhage, blind, peripheral vascular disease, limping, hyperuricemia.So must pay much attention to the harm of hyperlipidemia, positive prevention and treatment.
Blood lipid-lowering medicine is mainly Statins in the market.Such medicine has good lipid-lowering effect, but long-term taking, can be just like myalgia, muscle inflammation, even the musclar toxicity such as rhabdomyolysis, also can produce the side effect such as internal metabolism obstacle, gastrointestinal side effect, liver loss.Meanwhile, statins price is high, and long-term taking also can the corresponding financial burden that increases patient.
So, provide controlled, cheap, the eutherapeutic pure natural plant of a kind of safety to extract medicine, there is great social meaning and economic worth.
Summary of the invention
The object of this invention is to provide a kind of good effect, the more controlled Chinese medicine composition of quality.
Another object of the present invention is to provide a kind of preparation method of described Chinese medicine composition.
Another object of the present invention is to provide a kind ofly has the Chinese medicine composition of the market competitiveness in price equally.Described Chinese medicine composition can not increase because of the raising of curative effect patient's financial burden.
For achieving the above object, the present invention by the following technical solutions:
In drug composition of the present invention, comprise the percentage by weight component of following bulk drugs:
Folium Ginkgo 8%～87%, Flos Carthami 2%～79%, Lignum Dalbergiae Odoriferae 10%～27%;
Preferably, in Chinese medicine composition of the present invention, comprise the percentage by weight component of following bulk drugs:
Folium Ginkgo 23%～76%, Flos Carthami 5%～64%, Lignum Dalbergiae Odoriferae 12%～25%;
Preferred, in Chinese medicine composition of the present invention, comprise the percentage by weight component of following bulk drugs:
Folium Ginkgo 27%～63%, Flos Carthami 18%～55%, Lignum Dalbergiae Odoriferae 15%～23%;
Further preferred, in Chinese medicine composition of the present invention, comprise the percentage by weight component of following bulk drugs:
Folium Ginkgo 35%～47%, Flos Carthami 33%～45%, Lignum Dalbergiae Odoriferae 18%～21%;
Further preferred again, in Chinese medicine composition of the present invention, comprise the percentage by weight of following bulk drugs
Folium Ginkgo 45%, Flos Carthami 36%, Lignum Dalbergiae Odoriferae 19%.
The percentage by weight sum of Folium Ginkgo extract, Flos Carthami, Lignum Dalbergiae Odoriferae Radix Salviae Miltiorrhizae is 100%.
The preparation method of Folium Ginkgo of the present invention and Lignum Dalbergiae Odoriferae extract comprises the steps:
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and ethanol, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 50%～60% for the first time, and the volumetric concentration of eluting ethanol is 90%～95% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
Preferably, the volume ratio of described ethyl acetate and ethanol is 1:2.
Preferably, the volumetric concentration of eluting ethanol is 60% for the first time; The volumetric concentration of eluting ethanol is 90% for the second time.
Preferred, the preparation method of Folium Ginkgo of the present invention and Lignum Dalbergiae Odoriferae extract comprises the following steps:
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, the ethyl acetate and 70% ethanol that after being pulverized, add volume ratio 1:2, heating and refluxing extraction 2 times, 1～3 hour for the first time, 1～2 hour for the second time, filtrate merged to obtain extracting solution I, and medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5～1.5 hour for the first time, 0.5～1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with 50%～60% ethanol elution, then with 90%～95% ethanol elution, obtain eluent II, reclaim, concentrating under reduced pressure, dry, obtain.
Described elution flow rate is 1～4BV/h; Preferably, elution flow rate is 1～2BV/h, is more preferably 2BV/h.
Described macroporous resin is preferably AB-8 and X-5, more preferably X-5.
Concentration of alcohol described in the present invention is volumetric concentration.
In the present invention, Flos Carthami extract can be obtained on ，Ye Keyou market and be bought by prior art.Can also adopt following method preparation:
1. by raw material extracting in water 2 times, add for the first time 6～10 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5～3 hours, filter; Add for the second time 4～8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5～1.5 hour, filter merging filtrate;
2. the upper macroporous adsorbent resin of filtrate, water, 20%～60% ethanol eluting, 60%～95% ethanol eluting for the second time for the first time, collects eluent respectively;
3. steam pressure 0.01～0.09Mpa, vacuum-0.03～-0.08Mpa, 60～80 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum (50～55 ℃ of surveys);
4. vacuum-0.04～-0.09Mpa, 60～95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Preferably, step 2. in ethanol elution volumetric concentration be respectively: for the first time 30%～55%; For the second time 70%～90%; Preferred, the volumetric concentration of eluting ethanol is respectively: for the first time 35%; For the second time 75%.
Preferably, step is middle steam pressure 0.04～0.07Mpa 3., vacuum-0.04～-0.06Mpa, and 65～75 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum.
Preferred, step is middle steam pressure 0.05Mpa 3., vacuum-0.06Mpa, and temperature 70 C, is concentrated into relative density 1.23g/cm
3extractum.
Chinese medicine composition of the present invention, the various dosage forms that can be mixed as starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, glycine etc. with adjuvant on any or more than one pharmaceuticss, for example, can be made into aqueous injection, tablet, slow releasing tablet, drop pill, granule, injectable powder, capsule, microgranule.Preferred dosage form is drop pill, injectable powder.
In compositions of the present invention, can also in the situation that not affecting therapeutic purposes of the present invention and effect, add arbitrarily other compositions or the component in the present invention is equal to replacement, including but not limited to that Borneolum Syntheticum, Radix Salviae Miltiorrhizae are or/and Rhizoma Chuanxiong etc.As long as add the compositions obtaining after these compositions can also reach the therapeutic effect of the present composition, be all fine.
Chinese medicine composition of the present invention makes full use of the therapeutical effect of crude drug to cardiovascular disease, carries out scientific matching, produces coordinating effect, and more traditional ginkgo leaf extract preparation has better curative effect, and quality is more controlled, is applicable to suitability for industrialized production.
test example
Medicine blood fat reducing clinical observation of the present invention
1. data and method
1.1 physical data are selected hyperlipidemia patient 80 examples at random, male 40 examples, and female's 40 examples, at 44～76 years old age, average 58.6 years old, selecting 80 routine hyperlipidemia patients else was matched group, male 42 examples, female's 38 examples, 45～76 years old age, 58.1 years old mean age.
1.2 administrated method
Treatment group adopts the embodiment of the present invention 4 medicines, each 10mg, oral 3 times of every day, 2 months courses for the treatment of.Matched group gives Xuezhikang, each 10mg, every day 3 times, 2 months courses for the treatment of.Before treatment, withdraw other hypolipidemic.All patients before treatment, treatment does hematuria routine, blood fat, liver function, renal function, serum potassium, uranium, chlorine, blood glucose, Electrocardioscopy after January and the course for the treatment of finishing.
1.3 observation item
Before and after treatment, take out limosis vein blood and detect serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein, LDL (LDL-C), do liver function, renal function, blood, urine routine test simultaneously.
1.4 statistical procedures
Mean ± standard deviation for measurement data (
) represent, adopt t check; Enumeration data x
2check, P < 0.05 has significance for difference.
2. result
The curative effect of hyperlipidemia
Before and after table 1 liang group lipid therapy, compare (
)
Note: before and after * group internal therapy than P < 0.01, between # group after treatment than P < 0.01.
The present invention and Xuezhikang all have lipid-lowering effect, and present composition lipid-lowering effect is better than Xuezhikang.
The specific embodiment
embodiment 1
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 87%, Flos Carthami 3%, Lignum Dalbergiae Odoriferae 10%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1 hour for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 50% ethanol elution, then with 90% ethanol elution, elution flow rate is 1BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 20% ethanol elution, and then with 60%～95% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 60 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 2
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 8%, Flos Carthami 79%, Lignum Dalbergiae Odoriferae 13%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 95% ethanol elution, elution flow rate is 4BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 3
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 24%, Flos Carthami 64%, Lignum Dalbergiae Odoriferae 12%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1.5 hours for the first time, 1.5 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 55% ethanol elution, then with 95% ethanol elution, elution flow rate is 3BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 70% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.04Mpa, 65 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 70 C, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 4
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 76%, Flos Carthami 12%, Lignum Dalbergiae Odoriferae 12%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 90% ethanol elution, elution flow rate is 2BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 90% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.06Mpa, 75 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 5
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 45%, Flos Carthami 36%, Lignum Dalbergiae Odoriferae 19%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 55% ethanol elution, then with 95% ethanol elution, elution flow rate is 2BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 35% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.05Mpa, vacuum-0.06Mpa, temperature 70 C, is concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.06Mpa, 80 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 6
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 27%, Flos Carthami 55%, Lignum Dalbergiae Odoriferae 18%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 2 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 58% ethanol elution, then with 94% ethanol elution, elution flow rate is 3BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 50% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.06Mpa, 65 ℃ of temperature, are concentrated into relative density 1.21g/cm
3extractum; Vacuum-0.05Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 7
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 63%, Flos Carthami 18%, Lignum Dalbergiae Odoriferae 19%.
(1) Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material are got in the preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract, the ethyl acetate and 70% ethanol that after being pulverized, add volume ratio 1:2, heating extraction 2 times, 2.5 hours for the first time, 1.5 hours for the second time, filtrate merged to obtain extracting solution I, and medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 54% ethanol elution, then with 92% ethanol elution, elution flow rate is 1.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2.5 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 55% ethanol elution, collects eluent; Steam pressure 0.03Mpa, vacuum-0.04Mpa, 75 ℃ of temperature, are concentrated into relative density 1.24g/cm
3extractum; Vacuum-0.07Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 8
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 35%, Flos Carthami 45%, Lignum Dalbergiae Odoriferae 20%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 59% ethanol elution, then with 95% ethanol elution, elution flow rate is 3.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 59% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.08Mpa, vacuum-0.07Mpa, 78 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 9
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 47%, Flos Carthami 35%, Lignum Dalbergiae Odoriferae 18%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1 hour for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 51% ethanol elution, then with 91% ethanol elution, elution flow rate is 2.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 40% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, temperature 60 C, is concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.04Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 10
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 10%, Flos Carthami 75%, Lignum Dalbergiae Odoriferae 15%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1.5 hours for the first time, 1.5 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 56% ethanol elution, then with 94% ethanol elution, elution flow rate is 4BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 45% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.05Mpa, temperature 70 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 11
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 80%, Flos Carthami 8%, Lignum Dalbergiae Odoriferae 12%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 90% ethanol elution, elution flow rate is 2BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 25% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 70 C, is concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 12
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 75%, Flos Carthami 6%, Lignum Dalbergiae Odoriferae 19%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 2.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.2 hours for the first time, 0.8 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 50% ethanol elution, then with 95% ethanol elution, elution flow rate is 3BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 63 ℃ of temperature, are concentrated into relative density 1.21g/cm
3extractum; Vacuum-0.06Mpa, 85 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 13
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 67%, Flos Carthami 13%, Lignum Dalbergiae Odoriferae 20%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1 hour for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 57% ethanol elution, then with 92% ethanol elution, elution flow rate is 2.2BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.8 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 45% ethanol elution, and then uses 65% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.05Mpa, 78 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.06Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 14
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 50%, Flos Carthami 25%, Lignum Dalbergiae Odoriferae 25%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1 hour for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 50% ethanol elution, then with 90% ethanol elution, elution flow rate is 3.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 50% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 15
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 55%, Flos Carthami 27%, Lignum Dalbergiae Odoriferae 18%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5 hour for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 90%～95% ethanol elution, elution flow rate is 3BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 16
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 35%, Flos Carthami 38%, Lignum Dalbergiae Odoriferae 27%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 2.4 hours for the first time, 1.8 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.8 hour for the first time, 0.5 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 90% ethanol elution, elution flow rate is 1BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 2.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.04Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 17
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 20%, Flos Carthami 60%, Lignum Dalbergiae Odoriferae 20%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 0.51 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 55% ethanol elution, then with 90% ethanol elution, elution flow rate is 2.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.6 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 36% ethanol elution, and then uses 66% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.05Mpa, 66 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 18
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 15%, Flos Carthami 75%, Lignum Dalbergiae Odoriferae 10%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 2.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1 hour for the first time, 0.6 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin X-5 to carry out separation to it, first with water elution, obtain eluent I; Then with 60% ethanol elution, then with 95% ethanol elution, elution flow rate is 4BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.2 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 53% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.07Mpa, 65 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.04Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 19
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 60%, Flos Carthami 10%, Lignum Dalbergiae Odoriferae 20%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 1 hour for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 0.6 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 58% ethanol elution, then with 94% ethanol elution, elution flow rate is 3.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 51% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.05Mpa, vacuum-0.05Mpa, 65 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.05Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 20
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 85%, Flos Carthami 5%, Lignum Dalbergiae Odoriferae 10%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and 70% ethanol of volume ratio 1:2, heating extraction 2 times, 3 hours for the first time, 2 hours for the second time, filtrate merged to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 1.5 hours for the first time, 1 hour for the second time, filtrate merged to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt macroporous resin AB-8 to carry out separation to it, first with water elution, obtain eluent I; Then with 55% ethanol elution, then with 95% ethanol elution, elution flow rate is 1.5BV/h, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 20% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 21
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 78%, Flos Carthami 11%, Lignum Dalbergiae Odoriferae 11%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and ethanol, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 50%～60% for the first time, and the volumetric concentration of eluting ethanol is 90%～95% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 78% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 22
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 65%, Flos Carthami 8%, Lignum Dalbergiae Odoriferae 27%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, adding volume ratio is ethyl acetate and the ethanol of 1:2, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 55% for the first time, and the volumetric concentration of eluting ethanol is 90% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 90% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.05Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 23
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 76%, Flos Carthami 10%, Lignum Dalbergiae Odoriferae 14%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, adding volume ratio is ethyl acetate and the ethanol of 1:2, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 60% for the first time, and the volumetric concentration of eluting ethanol is 90% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.04Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
embodiment 24
According to following percentage by weight, get each raw material effective component extracting: Folium Ginkgo 21%, Flos Carthami 54%, Lignum Dalbergiae Odoriferae 25%.
(1) preparation of Folium Ginkgo and Lignum Dalbergiae Odoriferae extract
Get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, adding volume ratio is ethyl acetate and the ethanol of 1:2, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 55% for the first time, and the volumetric concentration of eluting ethanol is 95% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain.
(2) preparation of Flos Carthami extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 65% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 60 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, 85 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Get said medicine extract, add adjuvant and be prepared into Chinese medicine composition of the present invention.
Chinese medicine composition disclosed by the invention, those skilled in the art can extract link by change and realize.Embodiments of the invention are only the specific descriptions to technical scheme, are not the restrictions to technical scheme, and those skilled in the art all fall into protection scope of the present invention not changing the replacement done under the condition of flesh and blood of the present invention or change.
Claims (10)
1. a blood-fat reducing composition that contains Lignum Dalbergiae Odoriferae, comprises the raw material components of following percentage by weight: Folium Ginkgo 8%～87%, Flos Carthami 2%～79%, Lignum Dalbergiae Odoriferae 10%～27%.
2. compositions according to claim 1, is characterized in that, described constituent content is: Folium Ginkgo 23%～76%, Flos Carthami 5%～64%, Lignum Dalbergiae Odoriferae 12%～25%.
3. compositions according to claim 2, is characterized in that, described constituent content is: Folium Ginkgo 27%～63%, Flos Carthami 18%～55%, Lignum Dalbergiae Odoriferae 15%～23%.
4. compositions according to claim 1, is characterized in that, described constituent content is: Folium Ginkgo 35%～47%, Flos Carthami 33%～45%, Lignum Dalbergiae Odoriferae 18%～21%; Optimum is Folium Ginkgo 45%, Flos Carthami 36%, Lignum Dalbergiae Odoriferae 19%.
5. a preparation method for the compositions described in claim 1-4, comprises the steps:
(1) get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, after being pulverized, add ethyl acetate and ethanol, heating and refluxing extraction, obtain extracting solution I, medicinal residues add ethanol, and heating and refluxing extraction obtains extracting solution II, merge extractive liquid, I and II, be condensed into extractum, use dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I; Then with ethanol elution 2 times, the volumetric concentration of eluting ethanol is 50%～60% for the first time, and the volumetric concentration of eluting ethanol is 90%～95% for the second time, obtains eluent II, reclaim, and concentrating under reduced pressure, dry, obtain;
(2) Flos Carthami extract of preparing Flos Carthami extract or buying, adds the extract of step (1) according to formula, add adjuvant and be prepared into Chinese medicine composition of the present invention.
6. the preparation method of compositions according to claim 5, is characterized in that, the volume ratio of the ethyl acetate described in step (1) and ethanol is 1:2; The volumetric concentration of the described ethanol of eluting is for the first time 60%; The volumetric concentration of eluting ethanol is 90% for the second time.
7. according to the preparation method of the compositions described in claim 5 or 6, it is characterized in that, the preparation method of the extract described in step (1) is: get Folium Ginkgo and Lignum Dalbergiae Odoriferae medical material, the ethyl acetate and 70% ethanol that after being pulverized, add volume ratio 1:2, heating and refluxing extraction 2 times, 1～3 hour for the first time, 1～2 hour for the second time, filtrate merges to obtain extracting solution I, medicinal residues add 70% ethanol, heating and refluxing extraction 2 times, 0.5～1.5 hour for the first time, 0.5～1 hour for the second time, filtrate merges to obtain extracting solution II, merge extractive liquid, I and II, be condensed into extractum, with 50% dissolve with ethanol extractum, adopt nonpolar or low pole macroporous resin to carry out separation to it, first with water elution, obtain eluent I, then with 50%～60% ethanol elution, then with 90%～95% ethanol elution, obtain eluent II, reclaim, concentrating under reduced pressure, dry, obtain.
8. according to the preparation method of the compositions described in claim 5-7, it is characterized in that, the elution flow rate described in step (1) is 1～4BV/h; Preferably, elution flow rate is 1～2BV/h, is more preferably 2BV/h; Described macroporous resin is preferably AB-8 and X-5, more preferably X-5; The volumetric concentration of the described ethanol of eluting is for the first time 60%; The volumetric concentration of eluting ethanol is 90% for the second time.
9. according to the preparation method of the compositions described in claim 5-8, it is characterized in that, the described Flos Carthami extract preparation method of step (2) comprises the steps:
1. by raw material extracting in water 2 times, add for the first time 6～10 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5～3 hours, filter; Add for the second time 4～8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5～1.5 hour, filter merging filtrate;
2. the upper macroporous adsorbent resin of filtrate, water, 20%～60% ethanol eluting, 60%～95% ethanol eluting for the second time for the first time, collects eluent respectively;
3. steam pressure 0.01～0.09Mpa, vacuum-0.03～-0.08Mpa, 60～80 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum;
4. vacuum-0.04～-0.09Mpa, 60～95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
10. preparation method according to claim 9, is characterized in that, step 2. in ethanol elution volumetric concentration be respectively: for the first time 30%～55%; For the second time 70%～90%; Preferred, the volumetric concentration of eluting ethanol is respectively: for the first time 35%; For the second time 75%;
Step is middle steam pressure 0.04～0.07Mpa 3., vacuum-0.04～-0.06Mpa, and 65～75 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum; Preferred, steam pressure 0.05Mpa, vacuum-0.06Mpa, temperature 70 C, is concentrated into relative density 1.23g/cm
3extractum.
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|---|---|---|---|---|
| CN107158313A (en) * | 2017-04-28 | 2017-09-15 | 河南中医药大学第附属医院 | A kind of medicine for preventing and treating cardiovascular and cerebrovascular and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1356113A (en) * | 2000-11-28 | 2002-07-03 | 袁曙光 | Process for preparing Chinese medicine to treat cardiovascular and cerebrovascular diseases |
-
2012
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1356113A (en) * | 2000-11-28 | 2002-07-03 | 袁曙光 | Process for preparing Chinese medicine to treat cardiovascular and cerebrovascular diseases |
Non-Patent Citations (4)
| Title |
|---|
| 侯团章: "《中草药提取物 第1卷》", 31 March 2004 * |
| 刘成伦: "《天然药物化学》", 31 December 2011 * |
| 赵雪梅: "《高脂血症合理用药239问》", 28 February 2009 * |
| 顾觉奋: "《离子交换与吸附树脂在制药工业上的应用》", 31 March 2008 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107158313A (en) * | 2017-04-28 | 2017-09-15 | 河南中医药大学第附属医院 | A kind of medicine for preventing and treating cardiovascular and cerebrovascular and preparation method thereof |
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