CN103505482A - Blood lipid reducing composition containing sea-buckthorn and glossy ganoderma and preparation method of composition - Google Patents
Blood lipid reducing composition containing sea-buckthorn and glossy ganoderma and preparation method of composition Download PDFInfo
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- CN103505482A CN103505482A CN201210217221.5A CN201210217221A CN103505482A CN 103505482 A CN103505482 A CN 103505482A CN 201210217221 A CN201210217221 A CN 201210217221A CN 103505482 A CN103505482 A CN 103505482A
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- 150000002632 lipids Chemical class 0.000 title abstract description 5
- 235000003145 Hippophae rhamnoides Nutrition 0.000 title abstract 3
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Abstract
The invention discloses a blood lipid reducing composition containing sea-buckthorn and glossy ganoderma. The composition comprises the following active ingredients by weight percent: 10-85% of sea-buckthorn oil and 15-90% of glossy ganoderma extract. The invention also discloses a preparation method of the blood lipid reducing composition. The preparation method comprises the steps of extracting the active ingredients and preparing the finished product according to the formula. The blood lipid reducing composition makes the best of the treatment effects of active pharmaceutical ingredients on cardiovascular diseases, adopts scientific matching, generates synergistic effects, has better curative effects and more controllable quality than traditional preparations and is suitable for industrial production.
Description
Technical field
The Chinese medicine composition and the preparation method that the present invention relates to a kind of Cardiovarscular, belong to the field of Chinese medicines.
Background technology
Along with improving constantly of people's living standard, the change of dietary structure, aged tendency of population becomes clear day by day, and the sickness rate of coronary heart disease is day by day soaring.Coronary heart disease is because coronary atherosclerosis causes luminal stenosis, causes blood supply of cardiac muscle oxygen supply obstacle and the heart disease that causes, and " world killer " is known as.With regard to the whole world, since half a century, coronary heart disease has become the underlying cause of death of one of the disease that threat human health the is the most serious ，Shi U.S. and some industrialized country.According to World Health Organization's statistics, in the total death toll of the U.S., have 24.7% to die from coronary heart disease, Northern Ireland coronary heart disease case fatality rate occupies first place in the world, and is 5,36/,100,000; Japan is minimum, is 41,/10 ten thousand.In recent years, the M & M of China's coronary heart disease continues soaring, has every year at present 700,000 people of surpassing to die from coronary heart disease, is the modal a kind of cardiovascular diseases of middle-aged and elderly people, has leapt to as first of internal disease.Visible coronary heart disease has become global public hazards, and American wins cardiopathia for " pestilence in epoch ".
Hyperlipidemia is the risk factor of apoplexy, coronary heart disease, myocardial infarction, sudden death.In addition, hyperlipidemia is also to promote hypertension, an important risk factor of impaired glucose tolerance, diabetes.Hyperlipidemia also can cause fatty liver, liver cirrhosis, cholelithiasis, pancreatitis, retinal hemorrhage, blind, peripheral vascular disease, limping, hyperuricemia.So must pay much attention to the harm of hyperlipidemia, positive prevention and treatment.
Blood lipid-lowering medicine is mainly Statins in the market.Such medicine has good lipid-lowering effect, but long-term taking, can be just like myalgia, muscle inflammation, even the musclar toxicity such as rhabdomyolysis, also can produce the side effect such as internal metabolism obstacle, gastrointestinal side effect, liver loss.Meanwhile, statins price is high, and long-term taking also can the corresponding financial burden that increases patient.
So, provide controlled, cheap, the eutherapeutic pure natural plant of a kind of safety to extract medicine, there is great social meaning and economic worth.
Summary of the invention
The object of this invention is to provide a kind of good effect, the more controlled Chinese medicine composition of quality.
Another object of the present invention is to provide a kind of preparation method of described Chinese medicine composition.
Another object of the present invention is to provide a kind ofly has the Chinese medicine composition of the market competitiveness in price equally.Described Chinese medicine composition can not increase because of the raising of curative effect patient's financial burden.
For achieving the above object, the present invention by the following technical solutions:
In Chinese medicine composition of the present invention, comprise following percentage by weight component:
Oleum Hippophae 10～85%, Ganoderma extract 15～90%;
Preferably, in Chinese medicine composition of the present invention, comprise following percentage by weight component:
Oleum Hippophae 15～78%, Ganoderma extract 22～85%;
Preferred, in Chinese medicine composition of the present invention, comprise following percentage by weight component:
Oleum Hippophae 30～60%, Ganoderma extract 40～70%;
Further preferred, in Chinese medicine composition of the present invention, comprise following percentage by weight component:
Oleum Hippophae 40～55%, Ganoderma extract 45～60%;
Further preferred again, in Chinese medicine composition of the present invention, comprise following percentage by weight
Oleum Hippophae 53%, Ganoderma extract 47%.
In the present composition, the preparation method of Oleum Hippophae comprises the following steps:
The first static extracting 10min of 30～40MPa, dynamic extraction then, inlet pressure is 30～40MPa, outlet pressure is than the low 5～10kPa of inlet pressure; 15～40 ℃ of temperature, time 0.5～1h, CO
2volume 20-40kg/h.
Preferably, the first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 5kPa of inlet pressure; 25 ℃ of temperature, time 1h, CO
2volume 30kg/h.
In the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0～3.0 meter per seconds.
Oil yield can reach 10.57～12.31%.
Dynamic CO of the present invention
2oleum Hippophae prepared by extraction need not carry out separation again, free from admixture in oil, and the color of oil is pale yellow transparent, and fragrance is pure.
In the present invention, Ganoderma extract can be obtained on ，Ye Keyou market and be bought by prior art.Can also adopt following method preparation:
1. by raw material extracting in water 2 times, add for the first time 6～10 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5～3 hours, filter; Add for the second time 4～8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5～1.5 hour, filter merging filtrate;
2. the upper macroporous adsorbent resin of filtrate, water, 20%～60% ethanol eluting, 60%～95% ethanol eluting for the second time for the first time, collects eluent respectively;
3. steam pressure 0.01～0.09Mpa, vacuum-0.03～-0.08Mpa, 60～80 ℃ of temperature, are concentrated into the extractum (50～55 ℃ of surveys) of relative density 1.20～1.25g/cm3;
4. vacuum-0.04～-0.09Mpa, 60～95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Preferably, step 2. in ethanol elution volumetric concentration be respectively: for the first time 30%～55%; For the second time 70%～90%; Preferred, the volumetric concentration of eluting ethanol is respectively: for the first time 35%; For the second time 75%.
Preferably, step is middle steam pressure 0.04～0.07Mpa 3., vacuum-0.04～-0.06Mpa, and 65～75 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum.
Preferred, step 3. in steam pressure 0.05Mpa, vacuum-0.06Mpa, temperature 70 C, is concentrated into the extractum of relative density 1.23g/cm3.
Get above-mentioned Ganoderma extract, use appropriate dissolve with ethanol, join in saturated hydroxypropylβ-cyclodextrin solution, 25～40 ℃ stir after, then stir 0.5～2.5 hour, cold preservation is spent the night, sucking filtration, filtrate cold drying, obtains the clathrate powder of Ganoderma extract and hydroxypropylβ-cyclodextrin.
Chinese medicine composition of the present invention, the various dosage forms that can be mixed as starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, glycine etc. with adjuvant on any or more than one pharmaceuticss, for example, can be made into aqueous injection, tablet, slow releasing tablet, drop pill, granule, injectable powder, capsule, microgranule.Preferred dosage form is drop pill, injectable powder.
In compositions of the present invention, can also in the situation that not affecting therapeutic purposes of the present invention and effect, add arbitrarily other compositions or the component in the present invention is equal to replacement, including but not limited to that Borneolum Syntheticum, Radix Salviae Miltiorrhizae are or/and Rhizoma Chuanxiong etc.As long as add the compositions obtaining after these compositions can also reach the therapeutic effect of the present composition, be all fine.
Chinese medicine composition of the present invention makes full use of the therapeutical effect of crude drug to cardiovascular disease, carries out scientific matching, produces coordinating effect, and more traditional preparation has better curative effect, and quality is more controlled, is applicable to suitability for industrialized production.
test example
Medicine blood fat reducing clinical observation of the present invention
1. data and method
1.1 physical data are selected hyperlipidemia patient 80 examples at random, male 40 examples, and female's 40 examples, at 46～74 years old age, average 57.2 years old, selecting 80 routine hyperlipidemia patients else was matched group, male 40 examples, female's 40 examples, 42～75 years old age, 58.1 years old mean age.
1.2 administrated method
Treatment group adopts the embodiment of the present invention 4 medicines, each 10mg, oral 3 times of every day, 2 months courses for the treatment of.Matched group gives Xuezhikang, each 10mg, every day 3 times, 2 months courses for the treatment of.Before treatment, withdraw other hypolipidemic.All patients before treatment, treatment does hematuria routine, blood fat, liver function, renal function, serum potassium, uranium, chlorine, blood glucose, Electrocardioscopy after January and the course for the treatment of finishing.
1.3 observation item
Before and after treatment, take out limosis vein blood and detect serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL-C), low density lipoprotein, LDL (LDL-C), do liver function, renal function, blood, urine routine test simultaneously.
1.4 statistical procedures
Mean ± standard deviation for measurement data (
) represent, adopt t check; Enumeration data x
2check, P < 0.05 has significance for difference.
2. result
The curative effect of hyperlipidemia
Before and after table 1 liang group lipid therapy, compare (
)
Note: before and after * group internal therapy than P < 0.01, between # group after treatment than P < 0.01.
The present invention and Xuezhikang all have lipid-lowering effect, and present composition lipid-lowering effect is better than Xuezhikang.
The specific embodiment
embodiment 1
(1) preparation of Oleum Hippophae
The first static extracting 10min of 30MPa, dynamic extraction then, inlet pressure is 30MPa, outlet pressure is than the low 5kPa of inlet pressure; 15 ℃ of temperature, time 0.5h, CO
2volume 20kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0～3.0 meter per seconds.
Oil yield can reach 10.88%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 20% ethanol elution, and then with 60%～95% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 60 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 10%, Ganoderma extract 90%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 2
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 10kPa of inlet pressure; 40 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0～3.0 meter per seconds.
Oil yield can reach 11.68%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 85%, Ganoderma extract 15%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 3
(1) preparation of Oleum Hippophae
The first static extracting 10min of 30MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 10kPa of inlet pressure; 20 ℃ of temperature, time 0.5h, CO
2volume 20kg/h.
In the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0～3.0 meter per seconds.
Oil yield can reach 11.96%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 70% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.04Mpa, 65 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 70 C, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 15%, Ganoderma extract 85%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 4
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 5kPa of inlet pressure; 25 ℃ of temperature, time 1h, CO
2volume 30kg/h.
In the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 10Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 12.31%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 90% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.06Mpa, 75 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 53%, Ganoderma extract 47%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 5
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 30MPa, outlet pressure is than the low 10kPa of inlet pressure; 30 ℃ of temperature, time 1h, CO
2volume 30kg/h.
In the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.17%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 35% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.05Mpa, vacuum-0.06Mpa, temperature 70 C, is concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.06Mpa, 80 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 78%, Ganoderma extract 22%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 6
(1) preparation of Oleum Hippophae
The first static extracting 10min of 35MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 10kPa of inlet pressure; 35 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 2.0 meter per seconds.
Oil yield can reach 12.05%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 50% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.06Mpa, 65 ℃ of temperature, are concentrated into relative density 1.21g/cm
3extractum; Vacuum-0.05Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 30%, Ganoderma extract 70%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 7
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 5kPa of inlet pressure; 35 ℃ of temperature, time 1h, CO
2volume 25kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5Mpa, and periodically the flow speed wave moment of flow fluctuation is 2.0 meter per seconds.
Oil yield can reach 11.74%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2.5 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 55% ethanol elution, collects eluent; Steam pressure 0.03Mpa, vacuum-0.04Mpa, 75 ℃ of temperature, are concentrated into relative density 1.24g/cm
3extractum; Vacuum-0.07Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 60%, Ganoderma extract 40%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 8
(1) preparation of Oleum Hippophae
The first static extracting 10min of 30MPa, dynamic extraction then, inlet pressure is 30MPa, outlet pressure is than the low 6kPa of inlet pressure; 20 ℃ of temperature, time 1h, CO
2volume 40kg/h.
Oil yield can reach 10.71%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 59% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.08Mpa, vacuum-0.07Mpa, 78 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 40%, Ganoderma extract 60%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 9
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 30MPa, outlet pressure is than the low 8kPa of inlet pressure; 25 ℃ of temperature, time 1h, CO
2volume 25kg/h.
Oil yield can reach 10.94%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 40% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, temperature 60 C, is concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.04Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 55%, Ganoderma extract 45%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 10
(1) preparation of Oleum Hippophae
The first static extracting 10min of 28MPa, dynamic extraction then, inlet pressure is 28MPa, outlet pressure is than the low 5kPa of inlet pressure; 15 ℃ of temperature, time 1h, CO
2volume 28kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 8Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 12.15%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 45% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.05Mpa, temperature 70 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 20%, Ganoderma extract 80%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 11
(1) preparation of Oleum Hippophae
The first static extracting 10min of 38MPa, dynamic extraction then, inlet pressure is 38MPa, outlet pressure is than the low 7kPa of inlet pressure; 37 ℃ of temperature, time 1h, CO
2volume 38kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 9Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 12.30%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 25% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 70 C, is concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 80%, Ganoderma extract 20%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 12
(1) preparation of Oleum Hippophae
The first static extracting 10min of 35MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 10kPa of inlet pressure; 30 ℃ of temperature, time 1h, CO
2volume 25kg/h.
Oil yield can reach 10.98%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 63 ℃ of temperature, are concentrated into relative density 1.21g/cm
3extractum; Vacuum-0.06Mpa, 85 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 75%, Ganoderma extract 25%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 13
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 8kPa of inlet pressure; 20 ℃ of temperature, time 0.5h, CO
2volume 25kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 9Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.45%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.8 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 45% ethanol elution, and then uses 65% ethanol elution, collects eluent; Steam pressure 0.07Mpa, vacuum-0.05Mpa, 78 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.06Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 65%, Ganoderma extract 35%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 14
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 30～40MPa, outlet pressure is than the low 6kPa of inlet pressure; 36 ℃ of temperature, time 0.5h, CO
2volume 40kg/h.
Oil yield can reach 10.85%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 7 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 50% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 71%, Ganoderma extract 29%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 15
(1) preparation of Oleum Hippophae
The first static extracting 10min of 30MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 5kPa of inlet pressure; 30 ℃ of temperature, time 1h, CO
2volume 25kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 7Mpa, and periodically the flow speed wave moment of flow fluctuation is 2.5 meter per seconds.
Oil yield can reach 12.12%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 8 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 35%, Ganoderma extract 65%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 16
(1) preparation of Oleum Hippophae
The first static extracting 10min of 35MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 10kPa of inlet pressure; 33 ℃ of temperature, time 1h, CO
2volume 25kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 8Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.96%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 2.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.04Mpa, vacuum-0.04Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 64%, Ganoderma extract 36%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 17
(1) preparation of Oleum Hippophae
The first static extracting 10min of 35MPa, dynamic extraction then, inlet pressure is 30MPa, outlet pressure is than the low 7kPa of inlet pressure; 40 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 6Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0 meter per seconds.
Oil yield can reach 12.29%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.6 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 36% ethanol elution, and then uses 66% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.05Mpa, 66 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.08Mpa, 90 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 56%, Ganoderma extract 44%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 18
(1) preparation of Oleum Hippophae
The first static extracting 10min of 34MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 10kPa of inlet pressure; 38 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0 meter per seconds.
Oil yield can reach 12.26%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.2 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 53% ethanol elution, and then uses 85% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.07Mpa, 65 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.04Mpa, 75 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 62%, Ganoderma extract 38%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 19
(1) preparation of Oleum Hippophae
The first static extracting 10min of 32MPa, dynamic extraction then, inlet pressure is 32MPa, outlet pressure is than the low 5kPa of inlet pressure; 20 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 9Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.79%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 4 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 51% ethanol elution, and then uses 75% ethanol elution, collects eluent; Steam pressure 0.05Mpa, vacuum-0.05Mpa, 65 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.05Mpa, 65 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 22%, Ganoderma extract 78, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 20
(1) preparation of Oleum Hippophae
The first static extracting 10min of 40MPa, dynamic extraction then, inlet pressure is 40MPa, outlet pressure is than the low 5kPa of inlet pressure; 25 ℃ of temperature, time 0.5h, CO
2volume 25kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0 meter per seconds.
Oil yield can reach 11.63%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 6 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 20% ethanol elution, and then uses 95% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, temperature 60 C, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 38%, Ganoderma extract 62%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 21
(1) preparation of Oleum Hippophae
The first static extracting 10min of 32MPa, dynamic extraction then, inlet pressure is 32MPa, outlet pressure is than the low 9kPa of inlet pressure; 40 ℃ of temperature, time 1h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 12.01%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 10 times of water gagings, 90 ℃～100 ℃ of temperature keep 3 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 78% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.25g/cm
3extractum; Vacuum-0.09Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 72%, Ganoderma extract 28%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 22
(1) preparation of Oleum Hippophae
The first static extracting 10min of 35MPa, dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 10kPa of inlet pressure; 15 ℃ of temperature, time 0.5h, CO
2volume 40kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 10Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.74%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 9 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 55% ethanol elution, and then uses 90% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, 80 ℃ of temperature, are concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.05Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 67%, Ganoderma extract 33%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 23
(1) preparation of Oleum Hippophae
The first static extracting 10min of 34MPa, dynamic extraction then, inlet pressure is 34MPa, outlet pressure is than the low 10kPa of inlet pressure; 40 ℃ of temperature, time 1h, CO
2volume 35kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.39%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 6 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5 hours, filter; Add for the second time 8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1.5 hours, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 60% ethanol elution, and then uses 80% ethanol elution, collects eluent; Steam pressure 0.09Mpa, vacuum-0.08Mpa, 80 ℃ of temperature, are concentrated into relative density 1.23g/cm
3extractum; Vacuum-0.04Mpa, 95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 54%, Ganoderma extract 46%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
embodiment 24
(1) preparation of Oleum Hippophae
The first static extracting 10min of 39MPa, dynamic extraction then, inlet pressure is 39MPa, outlet pressure is than the low 10kPa of inlet pressure; 25 ℃ of temperature, time 1h, CO
2volume 30kg/h.
In the process of extraction, combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 10Mpa, and periodically the flow speed wave moment of flow fluctuation is 3.0 meter per seconds.
Oil yield can reach 11.89%.
(2) preparation of Ganoderma extract
By raw material extracting in water 2 times, add for the first time 7 times of water gagings, 90 ℃～100 ℃ of temperature keep 2 hours, filter; Add for the second time 5 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 1 hour, filter merging filtrate; Macroporous adsorbent resin on filtrate, first washes with water, then uses 30% ethanol elution, and then uses 65% ethanol elution, collects eluent; Steam pressure 0.01Mpa, vacuum-0.03Mpa, temperature 60 C, is concentrated into relative density 1.20g/cm
3extractum; Vacuum-0.04Mpa, 85 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
Take by weight Oleum Hippophae 50%, Ganoderma extract 50%, adds adjuvant and is prepared into Chinese medicine composition of the present invention.
Embodiments of the invention are only the specific descriptions to technical scheme, are not the restrictions to technical scheme, and those skilled in the art all fall into protection scope of the present invention not changing the replacement done under the condition of flesh and blood of the present invention or change.
Claims (10)
1. a blood-fat reducing composition that contains Fructus Hippophae Ganoderma, comprises the effective ingredient of following weight ratio: Oleum Hippophae 10～85%, Ganoderma extract 15～90%.
2. compositions according to claim 1, is characterized in that, comprises the effective ingredient of following weight ratio: Oleum Hippophae 15～78%, Ganoderma extract 22～85%.
3. compositions according to claim 2, is characterized in that, comprises the effective ingredient of following weight ratio: Oleum Hippophae 30～60%, Ganoderma extract 40～70%.
4. compositions according to claim 3, is characterized in that, comprises the effective ingredient of following weight ratio: Oleum Hippophae 40～55%, Ganoderma extract 45～60%.
5. compositions according to claim 1, is characterized in that, comprises the effective ingredient of following weight ratio: Oleum Hippophae 53%, Ganoderma extract 47%.
6. the preparation method of the Oleum Hippophae described in the arbitrary claim of claim 1～5, comprises the following steps: the first static extracting 10min of 30～40MPa, and dynamic extraction then, inlet pressure is 30～40MPa, outlet pressure is than the low 5～10kPa of inlet pressure; 15～40 ℃ of temperature, time 0.5～1h, CO
2volume 20-40kg/h.
7. preparation method according to claim 5, is characterized in that, comprises the following steps: the first static extracting 10min of 40MPa, and dynamic extraction then, inlet pressure is 35MPa, outlet pressure is than the low 5kPa of inlet pressure; 25 ℃ of temperature, time 1h, CO
2volume 30kg/h.
8. according to the preparation method described in claim 6 or 7, it is characterized in that, in the process of extraction separately or combine the following method that adopts: periodic pressure fluctuation, periodically flow fluctuation; Wherein the pressure wave moment of periodic pressure fluctuation is 5～10Mpa, and periodically the flow speed wave moment of flow fluctuation is 1.0～3.0 meter per seconds.
9. the preparation method of the Ganoderma extract described in the arbitrary claim of claim 1～5, comprises the following steps:
1. by raw material extracting in water 2 times, add for the first time 6～10 times of water gagings, 90 ℃～100 ℃ of temperature keep 1.5～3 hours, filter; Add for the second time 4～8 times of water gagings, 90 ℃～100 ℃ maintenances of temperature 0.5～1.5 hour, filter merging filtrate;
2. the upper macroporous adsorbent resin of filtrate, water, 20%～60% ethanol eluting, 60%～95% ethanol eluting for the second time for the first time, collects eluent respectively;
3. steam pressure 0.01～0.09Mpa, vacuum-0.03～-0.08Mpa, 60～80 ℃ of temperature, are concentrated into the extractum (50～55 ℃ of surveys) of relative density 1.20～1.25g/cm3;
4. vacuum-0.04～-0.09Mpa, 60～95 ℃ of temperature, dry, pulverize 60 mesh sieves, obtained.
10. preparation method according to claim 9, is characterized in that, step 2. in ethanol elution volumetric concentration be respectively: for the first time 30%～55%; For the second time 70%～90%; Preferred, the volumetric concentration of eluting ethanol is respectively: for the first time 35%; For the second time 75%;
Step is middle steam pressure 0.04～0.07Mpa 3., vacuum-0.04～-0.06Mpa, and 65～75 ℃ of temperature, are concentrated into relative density 1.20～1.25g/cm
3extractum; Preferred, steam pressure 0.05Mpa, vacuum-0.06Mpa, temperature 70 C, is concentrated into relative density 1.23g/cm
3extractum.
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