CN103483074B - Edible mushroom culture material and production method thereof - Google Patents

Edible mushroom culture material and production method thereof Download PDF

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Publication number
CN103483074B
CN103483074B CN201310418734.7A CN201310418734A CN103483074B CN 103483074 B CN103483074 B CN 103483074B CN 201310418734 A CN201310418734 A CN 201310418734A CN 103483074 B CN103483074 B CN 103483074B
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chinese medicine
medicine slag
root
culture
unslaked lime
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CN103483074A (en
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金茜
曾启华
朱彬
魏福能
林永
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Zunyi Normal University
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Zunyi Normal University
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Abstract

The invention relates to an edible mushroom culture material and a production method of the edible mushroom culture material. The edible mushroom culture material comprises, by weight, 30-100 parts of Chinese medicine residues, 10-60 parts of sorghum shells, 5-50 parts of corn flour and 1-15 parts of quick lime. Through the steps of smashing, sterilization, inoculation, fruiting and managing, edible mushrooms are cultivated. The cultivated edible mushrooms are free of poison and innocuousness, has edible safety and can solve the problem of waste treatment and achieve successes on environment and economy.

Description

The culture material of a kind of edible mushrooms and production method
Technical field
The present invention relates to food invention field, be specifically related to culture material and the production method of a kind of edible mushrooms.
Background technology
Edible mushrooms is that one is rich in the nutritive ingredients such as each seed amino acid, VITAMIN, mineral substance, polysaccharide, has higher nutritive value and dietary function; Edible fungi polysaccharide has the multiple efficacies such as anticancer, antitumor, antiviral, immunity moderation, often ediblely can build up health, and reduce the content of Blood Cholesterol, reducing blood pressure, is the food that people often eat, the huge market demand; Therefore, edible fungus culturing is also described as the sunrise industry of 21 century.
The method of current culture material and culturing edible fungus is various, if Chinese Patent Application No. is 201010127250.3 just to refer to Chinese medicine slag, cotton seed hulls, Semen Maydis powder, unslaked lime, nitrogenous fertilizer, potassium primary phosphate, Kemeiling, trace element and molysite as the culture material of edible mushrooms; Employ nitrogenous fertilizer, potassium primary phosphate, Mycosporin in this culture material, but these chemical substances are unfavorable to person health, the cotton polyphenol chemical constituents contained by the cotton seed hulls in culture material has impact to HUMAN HEALTH, and the quality of edible mushrooms is affected; Meanwhile, along with popularization and the expansion of edible fungus culturing, the edible fungus culturing such as cotton seed hull, the corn cob problem that there is lack of raw materials is more and more serious, causes edible fungus culturing cost more and more higher.
" edible fungi of china " 2012,31 6:66-67 page, describes during CN53-1054/Q ISSN1003-8310 is entitled as " research of jowar shell cultivation Pleurotus nebrodensis " and replaces cotton seed hulls to cultivate Pleurotus nebrodensis with jowar shell.But independent jowar shell is cultivated edible mushrooms and is sent out the problems such as bacterium speed is slow, mushroom growing up number is less, later stage output is not high.
Therefore be badly in need of solving culture medium of edible fungus and affect person health, edible mushrooms sends out the problems such as bacterium speed is slow, mushroom growing up number is less, later stage output is not high.
Chinese medicine slag derives from all kinds of herbal pharmaceutical factory, accounts for 70% of medicinal material total amount, is the primary solids waste of pharmaceutical factory discharge.Traditional Chinese medicine slag treatment process has two kinds: one to be concentrate as waste to stack or bury, and this not only takies a large amount of soils, produces a large amount of harmful fermentation gas, and also has pollution to underground water; Two is carried out drying rear burning by Chinese medicine slag, but to be greater than producing a large amount of pollutent such as flue dust, carbonic acid gas in the process that the dregs of a decoction burn, causes topsoil.So, how effectively Appropriate application to be carried out to Chinese medicine slag, become urgent problem.
The present invention, in order to solve the problem, has invented a kind of new culture material and production method.
Summary of the invention
The object of this invention is to provide the culture material of a kind of edible mushrooms.
Another object of the present invention is to provide the method utilizing culture material to produce edible mushrooms.
Another object of the present invention is to provide a kind of edible mushrooms.
The culture material of a kind of edible mushrooms provided by the invention, be made up of the raw material of following weight part: Chinese medicine slag 30-100 part, sorghum husk 10-60 part, Semen Maydis powder 5-50 part, unslaked lime 1-15 part, wherein said Chinese medicine slag refers to the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, White Mulberry Root-bark, Semen Armeniacae Amarum, Root-bark of Chinese Wolfberry, balloonflower root, Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Grosvenor Momordica, Loquat Leaf, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia, Rhizome of Fortune's Drynaria, thizoma curculiginis, Semen Sojae Preparatum, dark plum, Fruit of Cherokee Rose, c. faberii, rhizoma cibotii, bitter sweet leaf, Hu baldhead leaf, one or more dregs of a decoction after Chinese ephedra extraction.
Culture material provided by the invention, preferably be made up of the raw material of following weight part: Chinese medicine slag 35-95 part, sorghum husk 15-55 part, Semen Maydis powder 10-45 part, unslaked lime 2-14 part, wherein said Chinese medicine slag is: the Chinese medicine slag after White Mulberry Root-bark, Semen Armeniacae Amarum, Root-bark of Chinese Wolfberry, balloonflower root, Grosvenor Momordica, Loquat Leaf, cassia twig, Great Burdock Achene eight taste traditional Chinese medicine extraction.
Culture material provided by the invention, preferably be made up of the raw material of following weight part: Chinese medicine slag 40-90 part, sorghum husk 20-50 part, Semen Maydis powder 15-40 part, unslaked lime 3-12 part, wherein said Chinese medicine slag is: the Chinese medicine slag after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae eight taste traditional Chinese medicine extraction.
Culture material provided by the invention, more preferably be made up of the raw material of following weight part: Chinese medicine slag 50-80 part, sorghum husk 25-45 part, Semen Maydis powder 20-35 part, unslaked lime 5-10 part, wherein said Chinese medicine slag is: the Chinese medicine slag after Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Radix Glycyrrhizae, balloonflower root, Semen Sojae Preparatum, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati 12 taste traditional Chinese medicine extraction.
Culture material provided by the invention, more preferably be made up of the raw material of following weight part: Chinese medicine slag 55-75 part, sorghum husk 30-40 part, Semen Maydis powder 22-30 part, unslaked lime 6-9 part, wherein said Chinese medicine slag is: the Chinese medicine slag after Rhizome of Fortune's Drynaria, rhizoma cibotii, dark plum, Fruit of Cherokee Rose, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia eight taste traditional Chinese medicine extraction.
Culture material provided by the invention, the preferred raw material by following weight part forms further: Chinese medicine slag 60 parts, sorghum husk 35 parts, Semen Maydis powder 25 parts, unslaked lime 7 parts, and wherein said Chinese medicine slag is: the Chinese medicine slag after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, thizoma curculiginis, c. faberii, Chinese ephedra, recklessly baldhead's leaf, bitter sweet leaf 13 taste traditional Chinese medicine extraction.
One or more dregs of a decoction of the present invention are preferably through the dregs of a decoction of water extraction.
A kind of culture material provided by the invention produces the method for edible mushrooms, and with Chinese medicine slag, sorghum husk, Semen Maydis powder and unslaked lime for raw material, through pulverizing, sterilizing, inoculation, fruiting and management, concrete steps are as follows:
1) preparation of culture material: take weight part Chinese medicine slag, sorghum husk, Semen Maydis powder and unslaked lime for subsequent use;
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime;
3) the Chinese medicine slag culture material poach will regulated, waits heap fermentation 5-10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain edible fungus culturing material mixture;
4) constant-pressure and high-temperature sterilizing 6-8 hour is carried out after being packed by culture material mixture good for heap fermentation;
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 10-15%.
6) fruiting and management: during mycelial growth, control temperature is at 15-25 DEG C; Keep humidity at 60-90% during fruiting, temperature controls at 15-25 DEG C.
Culture material provided by the invention produces the method for edible mushrooms, and described is preferably 60-75% by the water content after the Chinese medicine slag culture material poach regulated.
A kind of edible mushrooms provided by the invention, is prepared from by following methods:
1) preparation of culture material: take weight part Chinese medicine slag, sorghum husk, Semen Maydis powder and unslaked lime for subsequent use;
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime;
3) the Chinese medicine slag culture material poach will regulated, makes water content be 60-75%, waits heap fermentation 5-10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain edible fungus culturing material mixture;
4) constant-pressure and high-temperature sterilizing 6-8 hour is carried out after being packed by culture material mixture good for heap fermentation;
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 10-15%.
6) fruiting and management: during mycelial growth, control temperature is at 15-25 DEG C; Keep humidity at 60-90% during fruiting, temperature controls at 15-25 DEG C.
The invention provides culture medium of edible fungus to have the following advantages:
1, compared with prior art:
1) culture material of the prior art is while in use the dregs of a decoction, Semen Maydis powder are raw material, and also containing nitrogenous fertilizer, potassium primary phosphate, Mycosporin chemical substance in culture material, these chemical substances are unfavorable to person health,
2) the cotton polyphenol chemical constituents contained by the cotton seed hulls in prior art culture material has impact to HUMAN HEALTH, and the quality of edible mushrooms is affected; Meanwhile, along with popularization and the expansion of edible fungus culturing, the edible fungus culturing such as cotton seed hull, the corn cob problem that there is lack of raw materials is more and more serious, causes edible fungus culturing cost more and more higher.
3) independent in prior art use jowar shell is cultivated edible mushrooms and is sent out the problems such as bacterium speed is slow, mushroom growing up number is less, later stage output is not high.
2, Chinese medicine slag, sorghum husk, Semen Maydis powder, unslaked lime combinationally use by the present invention, have the following advantages:
1) turn waste into wealth, save production cost: this technology successfully make use of the edible mushrooms that the mixing Chinese medicine slag discarded in pharmaceutical factory cultivates high-quality, instead of the contour cost feedstocks of a large amount of cotton seed hulls simultaneously, greatly reduces production cost.
2) edible mushrooms of this planting technology is nontoxic, edible safety: edible mushrooms mycelia in a bacterium process of present method cultivation is pure white, sturdy, a bacterium is fast and microbiological contamination rate is low, and fruit body of edible fungi is individual large, delicate, plump, and mushroom growing up number is many, and later stage output is high; Send product quality inspection to detect institute to sample after fruiting and carry out safety analysis detection, in the edible mushrooms utilizing the present invention to cultivate, the content of arsenic, lead, mercury meets national edible mushrooms hygienic standard (GB7096-2003), phenyl-hexachloride, dichlorodiphenyl trichloroethane etc. do not detect, therefore, national edible mushrooms hygienic standard is reached completely.
3) waste disposal problem is solved, realize environment and economy bumper harvests: the useless dregs of a decoction are greater than in stacking process can produce a large amount of peculiar smell and transudate, contaminated soil and air, process Chinese medicine slag refuse is also an expensive and difficult job of wasting time and energy, the invention solves the problem of environmental pollution and refuse treatment, create considerable economic benefit simultaneously.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
Culture material: White Mulberry Root-bark, Semen Armeniacae Amarum, Root-bark of Chinese Wolfberry, balloonflower root, Grosvenor Momordica, batch the Chinese medicine slag 65kg after leaf, cassia twig, Great Burdock Achene eight taste traditional Chinese medicine extraction, sorghum husk 25kg, Semen Maydis powder 10kg, unslaked lime 1kg.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 60%, waits heap fermentation 5 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 6 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 10%.
6) fruiting and management: control temperature 15 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 60% during fruiting, temperature controls at 15 DEG C.
Embodiment 2
Culture material: Chinese medicine slag 75kg, sorghum husk 20kg, Semen Maydis powder 5kg, unslaked lime 1kg after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae eight taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 75%, waits heap fermentation 10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 15%.
6) fruiting and management: control temperature 25 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 90% during fruiting, temperature controls at 25 DEG C.
Embodiment 3
Culture material: Chinese medicine slag 40kg, sorghum husk 20kg, Semen Maydis powder 15kg, unslaked lime 3kg after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae eight taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 75%, waits heap fermentation 10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 15%.
6) fruiting and management: control temperature 25 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 90% during fruiting, temperature controls at 25 DEG C.
Embodiment 4
Culture material: Chinese medicine slag 90kg, sorghum husk 50kg, Semen Maydis powder 40kg, unslaked lime 12kg after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae eight taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 75%, waits heap fermentation 10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 15%.
6) fruiting and management: control temperature 25 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 90% during fruiting, temperature controls at 25 DEG C.
Embodiment 5
Culture material: Chinese medicine slag 70kg, sorghum husk 25kg, Semen Maydis powder 5kg, unslaked lime 1kg after Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Radix Glycyrrhizae, balloonflower root, Semen Sojae Preparatum, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati 12 taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 65%, waits heap fermentation 7 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 7 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 12%.
6) fruiting and management: control temperature 20 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 70% during fruiting, temperature controls at 20 DEG C.
Embodiment 6
Culture material: Chinese medicine slag 80kg, sorghum husk 45kg, Semen Maydis powder 35kg, unslaked lime 10kg after Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Radix Glycyrrhizae, balloonflower root, Semen Sojae Preparatum, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati 12 taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 65%, waits heap fermentation 7 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 7 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 12%.
6) fruiting and management: control temperature 20 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 70% during fruiting, temperature controls at 20 DEG C.
Embodiment 7
Culture material: Chinese medicine slag 80kg, sorghum husk 15kg, Semen Maydis powder 5kg, unslaked lime 1kg after Rhizome of Fortune's Drynaria, rhizoma cibotii, dark plum, Fruit of Cherokee Rose, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia eight taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 8
Culture material: Chinese medicine slag 75kg, sorghum husk 40kg, Semen Maydis powder 30kg, unslaked lime 9kg after Rhizome of Fortune's Drynaria, rhizoma cibotii, dark plum, Fruit of Cherokee Rose, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia eight taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 9
Culture material: Chinese medicine slag 40kg, sorghum husk 20kg, Semen Maydis powder 15kg, unslaked lime 3kg after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, thizoma curculiginis, c. faberii, Chinese ephedra, recklessly baldhead's leaf, bitter sweet leaf 13 taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 10
Culture material: Chinese medicine slag 75kg, sorghum husk 20kg, Semen Maydis powder 5kg, unslaked lime 1kg after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, thizoma curculiginis, c. faberii, Chinese ephedra, recklessly baldhead's leaf, bitter sweet leaf 13 taste traditional Chinese medicine extraction.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 11
Culture material: Chinese medicine slag 60kg, sorghum husk 35kg, Semen Maydis powder 25kg, unslaked lime 1.5kg after Great Burdock Achene extracts.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 12
Culture material: Chinese medicine slag 75kg, sorghum husk 20kg, Semen Maydis powder 15kg, unslaked lime 1.5kg after Wu Chia Pee extracts.
Preparation method:
1) take the Chinese medicine slag of weight part, sorghum husk, Semen Maydis powder, unslaked lime are for subsequent use.
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime.
3) the Chinese medicine slag culture material poach will regulated, makes water content reach 70%, waits heap fermentation 8 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain culture medium of edible fungus mixture.
4) constant-pressure and high-temperature sterilizing is carried out 8 hours after being packed by culture material mixture good for heap fermentation.
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 13%.
6) fruiting and management: control temperature 22 DEG C during control temperature mycelial growth during mycelial growth; Keep humidity 80% during fruiting, temperature controls at 22 DEG C.
Embodiment 13
Safety analysis detects
Safety analysis detection (sending product quality inspection to detect institute to detect) is carried out to sample after fruiting, in the edible mushrooms utilizing the present invention to cultivate, the content of arsenic, lead, mercury meets national edible mushrooms hygienic standard (GB7096-2003), phenyl-hexachloride, dichlorodiphenyl trichloroethane etc. do not detect, therefore, national edible mushrooms hygienic standard is reached completely.
Comparative example 1: with composition and method in 201010127250.3 embodiments 2
Culture material: Chinese medicine slag 63 parts, cotton seed hulls 20 parts, Semen Maydis powder 5 parts, unslaked lime 3 parts, 6.6 parts, ammonium nitrate, potassium primary phosphate 2.3 parts, Kemeiling 0.1 part, trace element 0.5 part and 0.3 part, molysite.
Preparation method:
The first step, by Chinese medicine slag dry, pack;
Second step, be divided into main raw material with Chinese medicine slag, cotton seed hulls, corn, with unslaked lime, nitrogenous fertilizer, potassium primary phosphate, trace element, molysite and Kemeiling for auxiliary material is prepared burden;
3rd step, major ingredient and auxiliary material are mixed, add water, material-water ratio is 1:1.5;
4th step, then build heap fermentation 12d, taper heap diameter 4m, high 2.5m, during the fermentation stirring 4 times.
5th step, the planting material of fermenting to be loaded in plastics bag, carry out autoclaving, inoculate after cooling completely.
Comparative example 2: with " edible fungi of china " 2012,31 6:66-67 page, the composition in CN53-1054/QISSN 1003-8310 and method
Culture material: jowar shell, unslaked lime
Preparation method:
1) jowar shell impurity elimination, adjusts ph to 7.0-7.5 with unslaked lime, pack, sterilizing;
2) inoculate: access is female in the transfer room fumigated in advance plants, often prop up female test tube of planting and connect 5 bags of original seed material;
3) cultivate: female for access original seed Dai Zhi culturing room of planting cultivated, culture temperature is 26 DEG C, after cultivating 5d, start to observe and record bacterial classification situation.
Embodiment 14 simultaneous test
1, comparative example 1,2 and embodiment 1,2,5,7,9,11,12 are carried out Primary spawn as culture material, the results are shown in Table 1.
Table 1 different material cultivates the development condition of original seed
Group Mycelia germinates number of days/d The full bottle number of days/d of mycelia Mycelia sends out the situation of filling
Comparative example 1 1 20 Mycelia is pure white, sturdy
Comparative example 2 2 22 Mycelia is pure white, sturdy
Embodiment 1 1.5 19 Mycelia is pure white, sturdy
Embodiment 2 1 17 Mycelia is pure white, sturdy
Embodiment 5 1 18 Mycelia is pure white, sturdy
Embodiment 7 1 17 Mycelia is pure white, sturdy
Embodiment 9 1 19 Mycelia is pure white, sturdy
Embodiment 11 1 18 Mycelia is pure white, sturdy
Embodiment 12 1 17 Mycelia is pure white, sturdy
Table 1 shows embodiment and comparative example in hyphal development situation difference not quite, but the hyphal development number of days of embodiment is obviously few than the number of days of comparative example.The Primary spawn time can be shortened.
2, comparative example 1,2 is done to cultivate comparing of cultivar with embodiment 1,2,5,7,9,11,12, the results are shown in Table 2.
Table 2 different material cultivates the comparison of cultivar
Group Mycelia germinates number of days/d Mycelia purseful number of days/d Mycelia sends out the situation of filling
Comparative example 1 1 45 Mycelia is pure white, sturdy
Comparative example 2 2 47 Mycelia is pure white, sturdy
Embodiment 1 2 37 Mycelia is pure white, sturdy
Embodiment 2 1 33 Mycelia is pure white, sturdy
Embodiment 5 1 36 Mycelia is pure white, sturdy
Embodiment 7 1 33 Mycelia is pure white, sturdy
Embodiment 9 1.5 35 Mycelia is pure white, sturdy
Embodiment 11 1 34 Mycelia is pure white, sturdy
Embodiment 12 1 34 Mycelia is pure white, sturdy
Table 2 shows embodiment and comparative example process hyphal development situation difference not quite, but the hyphal development number of days of embodiment is obviously few than the number of days of comparative example process.Embodiment obviously shortens the incubation time of cultivar.
3, comparative example 1,2 and embodiment 1,2,5,7,9,11,12 are made fruiting simultaneous test, the results are shown in Table 3.
Table 3 different material culturing edible fungus fruiting compares
Group Cell age phase/d Growth cycle/d Productive rate (g. bag -1
Comparative example 1 68 90 395
Comparative example 2 67 92 401
Embodiment 1 51 120 480
Embodiment 2 50 121 475
Embodiment 5 52 119 482
Embodiment 7 51 122 470
Embodiment 9 53 117 472
Embodiment 11 52 119 468
Embodiment 12 53 118 462
Table 3 shows that the cell age phase of embodiment culturing edible fungus obviously shortens, and growth cycle obviously extends, and productive rate significantly improves.
4, embodiment and comparative example culture material are gathered the main quality index of edible mushrooms, in table 4.
The instruction of table 4 main quality is compared
Table 4 shows, the main quality index of embodiment is all obviously better than the edible mushrooms of comparative example.
5, embodiment and comparative example edible fungus living being Indexes Comparison, in table 5.
Table 5 primary biological Indexes Comparison
Group Bacteria developing period/d Biological transformation ratio % Production cost/unit
Comparative example 1 47 75 1.5
Comparative example 2 46 72 1.0
Embodiment 1 36 92 0.9
Embodiment 2 35 87 0.9
Embodiment 5 35.5 89 0.9
Embodiment 7 37 87 0.9
Embodiment 9 36 85 0.9
Embodiment 11 36.5 87 0.7
Embodiment 12 35.9 91 0.7
Table 5 shows, embodiment is compared with comparative example, and embodiment is starkly lower than comparative example at bacteria developing period, and biological transformation ratio is apparently higher than comparative example, and cost is lower than comparative example.
Conclusion: no matter the present invention is obviously better than comparative example from the aspect such as quality or Biological indicators, and is make use of waste material, and production cost significantly reduces, and improves economic benefit, can reduce the generation of refuse, alleviate environmental pollution, fundamentally solves dregs of a decoction emission problem.
Although above with general explanation, embodiment and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.

Claims (7)

1. the culture material of an edible mushrooms, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 30-100 part, sorghum husk 10-60 part, Semen Maydis powder 5-50 part, unslaked lime 1-15 part, wherein said Chinese medicine slag refers to the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, White Mulberry Root-bark, Semen Armeniacae Amarum, Root-bark of Chinese Wolfberry, balloonflower root, Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Grosvenor Momordica, Loquat Leaf, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia, Rhizome of Fortune's Drynaria, thizoma curculiginis, Semen Sojae Preparatum, dark plum, Fruit of Cherokee Rose, c. faberii, rhizoma cibotii, bitter sweet leaf, Hu baldhead leaf, one or more dregs of a decoction after Chinese ephedra extraction,
Described culture material is prepared from through following methods:
1) preparation of culture material: take weight part Chinese medicine slag, sorghum husk, Semen Maydis powder and unslaked lime for subsequent use;
2) Chinese medicine slag is mixed with sorghum husk, Semen Maydis powder, regulate planting material pH to be neutral with unslaked lime;
3) the Chinese medicine slag culture material poach will regulated, makes water content be 60-75%, waits heap fermentation 5-10 days, and pedestrian's work of going forward side by side or mechanical stirring, obtain edible fungus culturing material mixture;
4) constant-pressure and high-temperature sterilizing 6-8 hour is carried out after being packed by culture material mixture good for heap fermentation;
5) the bacterium bag after high-temperature sterilization is chilled to room temperature, aseptically adopt two ends inoculation method to carry out edible fungi inoculation, inoculum size is 10-15%;
6) fruiting and management: during mycelial growth, control temperature is at 15-25 DEG C; Keep humidity at 60-90% during fruiting, temperature controls at 15-25 DEG C.
2. culture material according to claim 1, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 35-95 part, sorghum husk 15-55 part, Semen Maydis powder 10-45 part, unslaked lime 2-14 part, wherein said Chinese medicine slag is: the Chinese medicine slag after White Mulberry Root-bark, Semen Armeniacae Amarum, Root-bark of Chinese Wolfberry, balloonflower root, Grosvenor Momordica, Loquat Leaf, cassia twig, Great Burdock Achene eight taste traditional Chinese medicine extraction.
3. culture material according to claim 1, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 40-90 part, sorghum husk 20-50 part, Semen Maydis powder 15-40 part, unslaked lime 3-12 part, wherein said Chinese medicine slag is: the Chinese medicine slag after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae eight taste traditional Chinese medicine extraction.
4. culture material according to claim 1, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 50-80 part, sorghum husk 25-45 part, Semen Maydis powder 20-35 part, unslaked lime 5-10 part, wherein said Chinese medicine slag is: the Chinese medicine slag after Japanese Honeysuckle, the capsule of weeping forsythia, schizonepeta, Herba Lophatheri, reed rhizome, Radix Glycyrrhizae, balloonflower root, Semen Sojae Preparatum, Cynanchum glaucescens, the tuber of stemona, windproof, Semen Astragali Complanati 12 taste traditional Chinese medicine extraction.
5. culture material according to claim 1, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 55-75 part, sorghum husk 30-40 part, Semen Maydis powder 22-30 part, unslaked lime 6-9 part, wherein said Chinese medicine slag is: the Chinese medicine slag after Rhizome of Fortune's Drynaria, rhizoma cibotii, dark plum, Fruit of Cherokee Rose, sealwort, Tuber Fleeceflower Root, mulberries, Psoralea corylifolia eight taste traditional Chinese medicine extraction.
6. culture material according to claim 1, it is characterized in that, be made up of the raw material of following weight part: Chinese medicine slag 60 parts, sorghum husk 35 parts, Semen Maydis powder 25 parts, unslaked lime 7 parts, wherein said Chinese medicine slag is: the Chinese medicine slag after the root of herbaceous peony, Wu Chia Pee, cassia twig, Great Burdock Achene, Root of Glaucescent Fissistigma, the root of large-flowered skullcap, perilla seed, Radix Glycyrrhizae, thizoma curculiginis, c. faberii, Chinese ephedra, recklessly baldhead's leaf, bitter sweet leaf 13 taste traditional Chinese medicine extraction.
7. the culture material according to any one of claim 1-6, is characterized in that, one or more described Chinese medicine bits are through the dregs of a decoction after water extraction.
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