CN103461401A - Natural fungicide for preventing and treating gingko wilt disease and preparation method thereof - Google Patents
Natural fungicide for preventing and treating gingko wilt disease and preparation method thereof Download PDFInfo
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- CN103461401A CN103461401A CN201310447300XA CN201310447300A CN103461401A CN 103461401 A CN103461401 A CN 103461401A CN 201310447300X A CN201310447300X A CN 201310447300XA CN 201310447300 A CN201310447300 A CN 201310447300A CN 103461401 A CN103461401 A CN 103461401A
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Abstract
The invention provides a natural fungicide for preventing and treating the gingko wilt disease. The natural fungicide for preventing and treating the gingko wilt disease comprises herba siegesbeckiae, sealwort, granatum, Chinese mosla herb, coptidis rhizome, fortune eupatorium herb, mint, rosa laevigata michx, folium ilicis purpurea and compound minerals. The natural fungicide for preventing and treating the gingko wilt disease is rich in raw material source, is low in cost, is made of purely natural components, is free of toxin and pollution, and can effectively prevent and treat the gingko wilt disease.
Description
Technical field
The invention belongs to the prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating ginkgo dead leaf disease and preparation method thereof.
Background technology
Ginkgo dead leaf disease is one of Major Diseases of ginkgo, in Guangxi, the ginkgoes such as Zhejiang, Jiangsu, Shandong concentrate producing region that generation is in various degree arranged.The morbidity of compare Xin producing region, general old producing region is serious.Susceptible plant, the lighter's partial blade shifts to an earlier date withered coming off, and the severe one blade is de-light all, thereby cause tree vigo(u)r weak, retarded growth.The sick sick initial stage common blade tip flavescence of ginkgo dead leaf, between June, browning is withered gradually at yellow position, and by local expansion to whole leaf margin, be extremely leaf margin scab russet of brown.Thereafter, scab spreads to blade base gradually, until whole blade overstrike or taupe, till dried-up coming off.7-August, the boundary of scab and health tissues was obvious, and the scab edge is corrugated, and color is darker, and its peripheral edge portion is also shown in narrower or wider foresythia tape.Rise September, and scab obviously increases, and uneven phenomenon appears in diffusion edge, and sick strong boundary of organization is also gradually not obvious.In addition 9～October nursery stock or the blade of setting greatly the base portion coppice shoot produce some irregular faded spots on indefinite position, the center brown, though these spots wood obviously enlarge, the leaf margin spot phase Colaesce of normal and extension.The generation reason of ginkgo seedling damping off it has been generally acknowledged that by chain lattice spore alternariaalternata (fr.) Keissl, enclose small cluster shell glomereltacingulate (Stonem) Spauld.etSchrenk and Pestalotiaginkgo (Pestalotia ginkgo hori) infects and causes, and is difficult for prevention and control.
For control ginkgo dead leaf is sick normally used, be the chemical pesticide bactericide, because a large amount of problem of environmental pollutions that use chemical pesticide to cause are on the rise.Simultaneously, adopt the chemical pesticide bactericide, make various phytopathogen bacterial classifications produce drug resistance, pesticide resistance, increased the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, to useful biology, can threaten yet.And a lot of chemical pesticides are non-degradables or are difficult to degraded, easily at the animals and plants cylinder accumulation, pollute the enrichment of thing.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", but also increase gradually to the ecological agricultural chemical demand of pollution-free natural degradation in market.From the development of pesticide science, on pesticide manufacturing is used, " Harmless " of " back to nature " and agricultural chemicals, be the inexorable trend of society and Development of Natural Science, so the new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the purpose of this invention is to provide a kind of environmental protection, control ginkgo dead leaf disease that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating ginkgo dead leaf disease provided by the invention, make: pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly, compound many ore deposits by following component.
Preferably, make: pig label grass 4-6 part, sealwort 4-6 part, granatum 4-6 part, elscholtiza 4-6 part, coptis 2-4 part, eupatorium 1-3 part, peppermint 1-3 part, fruit of Cherokee rose 1-3 part, Chinese holly 0.5-1.5 part, compound many ore deposits 1-3 part by the component of following weight portion.
More preferably, make 5 parts of: pig label grass, 5 parts of sealworts, 5 parts of granatums, 5 parts of elscholtizas, 3 parts of the coptiss, 2 parts of eupatoriums, 2 parts of peppermints, 2 parts of the fruits of Cherokee rose, 1 part of Chinese holly, 2 parts, compound many ore deposits by the component of following weight portion.
Wherein, described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, and the compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams, surplus is the elements such as carbon, oxygen, nitrogen, phosphorus.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control ginkgo dead leaf disease, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
The present invention also provides the application of natural bactericidal agent in control ginkgo dead leaf disease of above-mentioned control ginkgo dead leaf disease, and be specially: the natural bactericidal agent that will prevent and treat ginkgo dead leaf disease mixes and is the sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control ginkgo dead leaf disease provided by the invention are abundant, with low cost, adopt the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat ginkgo dead leaf disease.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, the described concrete material proportion of embodiment, process conditions and result thereof be only for the present invention is described, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control ginkgo dead leaf disease, make 4 parts of: pig label grass, 5 parts of sealworts, 4 parts of granatums, 5 parts of elscholtizas, 2 parts of the coptiss, 1 part of eupatorium, 3 parts of peppermints, 3 parts of the fruits of Cherokee rose, 1.5 parts of Chinese hollies, 2 parts, compound many ore deposits by the component of following weight portion.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added to the ethanol water of 8 times of amounts 95%, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/15 of total amount, adds the water of 2 times of volumes of concentrate, standing 36h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Embodiment 2
The natural bactericidal agent of control ginkgo dead leaf disease, make 6 parts of: pig label grass, 6 parts of sealworts, 6 parts of granatums, 4 parts of elscholtizas, 4 parts of the coptiss, 3 parts of eupatoriums, 1 part of peppermint, 1 part of the fruit of Cherokee rose, 0.5 part of Chinese holly, 1 part, compound many ore deposits by the component of following weight portion.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added to the ethanol water of 5 times of amounts 95%, be heated to 80 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 80 ℃ and decocts 4h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/20 of total amount, adds the water of 1 times of volume of concentrate, standing 48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
Embodiment 3
The natural bactericidal agent of control ginkgo dead leaf disease, make 5 parts of: pig label grass, 4 parts of sealworts, 5 parts of granatums, 6 parts of elscholtizas, 3 parts of the coptiss, 2 parts of eupatoriums, 2 parts of peppermints, 2 parts of the fruits of Cherokee rose, 1 part of Chinese holly, 3 parts, compound many ore deposits by the component of following weight portion.The compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
Its preparation method comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added to the ethanol water of 10 times of amounts 95%, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate, be concentrated into ethanol and add 1/10 of total amount, adds the water of 3 times of volumes of concentrate, standing 24h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
The inhibition activity of the natural bactericidal agent of the control ginkgo dead leaf disease of embodiment 4 mensuration embodiment 1 to 3 to the strawberry pathogen
This experiment is in vitro, measures the natural bactericidal agent of control ginkgo dead leaf disease of embodiment 1 to 3 to the inhibition activity of strawberry pathogen, and method and result are as follows:
1. for the examination bacterial classification
Chain lattice spore alternariaalternata (fr.) Keissl is commercially available
Enclose small cluster shell glomereltacingulate (Stonem) Spauld.etSchrenk commercially available
(Pestalotia ginkgo hori) is commercially available for Pestalotiaginkgo
2.EC50 measure
Utilize mycelial growth rate method and spore germination method to measure the EC50 value of embodiment of the present invention 1-3 natural bactericidal agent to above-mentioned germ, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the EC50 value for the examination pathogen
Adopt method of regulation in " pesticide field efficacy medicine test criterion ", the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3 is tested, diluted concentration is 500 and 1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment ginkgo dead leaf disease of embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (6)
1. a natural bactericidal agent of preventing and treating ginkgo dead leaf disease, is characterized in that: by following component, made: pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly, compound many ore deposits.
2. a kind of natural bactericidal agent of preventing and treating ginkgo dead leaf disease according to claim 1, is characterized in that: by the component of following weight portion, make: pig label grass 4-6 part, sealwort 4-6 part, granatum 4-6 part, elscholtiza 4-6 part, coptis 2-4 part, eupatorium 1-3 part, peppermint 1-3 part, fruit of Cherokee rose 1-3 part, Chinese holly 0.5-1.5 part, compound many ore deposits 1-3 part.
3. a kind of natural bactericidal agent of preventing and treating ginkgo dead leaf disease according to claim 1, is characterized in that: by the component of following weight portion, make 5 parts of: pig label grass, 5 parts of sealworts, 5 parts of granatums, 5 parts of elscholtizas, 3 parts of the coptiss, 2 parts of eupatoriums, 2 parts of peppermints, 2 parts of the fruits of Cherokee rose, 1 part of Chinese holly, 2 parts, compound many ore deposits.
4. a kind of natural bactericidal agent of preventing and treating ginkgo dead leaf disease according to claim 1, it is characterized in that: described compound many ore deposits comprise boron, zinc, molybdenum, manganese, copper, iron, and the compound many ore deposits of per kilogram comprise: boron 20 grams, zinc 30 grams, molybdenum 50 grams, manganese 50 grams, copper 20 grams, iron 100 grams.
5. a preparation method who prevents and treats the natural bactericidal agent of ginkgo dead leaf disease is characterized in that: comprise the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material Wei pig label grass, sealwort, granatum, elscholtiza, the coptis, eupatorium, peppermint, the fruit of Cherokee rose, Chinese holly;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-80 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-80 ℃ and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, standing 24-48h;
(5) filter, filtrate is concentrated into without the alcohol flavor, adds the water-soluble solution of same weight, adds compound many ore deposits to mix, and must prevent and treat the natural bactericidal agent of ginkgo dead leaf disease.
6. the application of the natural bactericidal agent of control ginkgo dead leaf disease in control ginkgo dead leaf disease, it is characterized in that: the natural bactericidal agent that will prevent and treat ginkgo dead leaf disease mixes and is the sterilization microbial inoculum with 500-1000 times of water gaging.
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| CN201310447300.XA CN103461401B (en) | 2013-09-26 | 2013-09-26 | A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996489A (en) * | 2015-06-03 | 2015-10-28 | 广西大学 | Natural bactericide for preventing and controlling pepper anthracnose |
| CN105409657A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Disease and insect pest control method for Coriandrum sativum |
| CN107624821A (en) * | 2017-09-27 | 2018-01-26 | 贵州蓝天生态农业科技发展有限公司 | A kind of composition for treating Momordica grosvenori southern blight |
| CN108551961A (en) * | 2018-05-15 | 2018-09-21 | 务川县文娟农业开发有限公司 | A method of prevention podocarpus dead leaf disease |
| CN112174728A (en) * | 2020-09-27 | 2021-01-05 | 中科美兰（合肥）生物工程有限公司 | Biological source multifunctional water-soluble pesticide fertilizer special for ginkgo leaf blight |
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| CN1194843A (en) * | 1997-04-01 | 1998-10-07 | 李恩洪 | Method for producing green health medicine |
| CN1895291A (en) * | 2006-06-09 | 2007-01-17 | 陈永丽 | Nano-Chinese medicinal biological product and its preparation |
| CN102845485A (en) * | 2012-10-10 | 2013-01-02 | 张聿民 | Chinese medicinal extract disinfectant |
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2013
- 2013-09-26 CN CN201310447300.XA patent/CN103461401B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1194843A (en) * | 1997-04-01 | 1998-10-07 | 李恩洪 | Method for producing green health medicine |
| CN1895291A (en) * | 2006-06-09 | 2007-01-17 | 陈永丽 | Nano-Chinese medicinal biological product and its preparation |
| CN102845485A (en) * | 2012-10-10 | 2013-01-02 | 张聿民 | Chinese medicinal extract disinfectant |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996489A (en) * | 2015-06-03 | 2015-10-28 | 广西大学 | Natural bactericide for preventing and controlling pepper anthracnose |
| CN105409657A (en) * | 2015-11-26 | 2016-03-23 | 全椒县桃花湾果蔬种植专业合作社 | Disease and insect pest control method for Coriandrum sativum |
| CN107624821A (en) * | 2017-09-27 | 2018-01-26 | 贵州蓝天生态农业科技发展有限公司 | A kind of composition for treating Momordica grosvenori southern blight |
| CN108551961A (en) * | 2018-05-15 | 2018-09-21 | 务川县文娟农业开发有限公司 | A method of prevention podocarpus dead leaf disease |
| CN112174728A (en) * | 2020-09-27 | 2021-01-05 | 中科美兰（合肥）生物工程有限公司 | Biological source multifunctional water-soluble pesticide fertilizer special for ginkgo leaf blight |
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| CN103461401B (en) | 2015-08-05 |
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