CN103392601B - Michelia compressa tissue culture propagation method - Google Patents
Michelia compressa tissue culture propagation method Download PDFInfo
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- CN103392601B CN103392601B CN201310344980.2A CN201310344980A CN103392601B CN 103392601 B CN103392601 B CN 103392601B CN 201310344980 A CN201310344980 A CN 201310344980A CN 103392601 B CN103392601 B CN 103392601B
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Abstract
The invention discloses a michelia compressa tissue culture propagation method. The method includes the following steps: culturing explant plants; selecting the explants and disinfecting; culturing by an improved WPW culture medium; after cutting the treated explants, carrying out axillary bud early-generation induction culture; carrying out high voltage electrostatic field treatment; carrying out transgenerational multiplication culture; carrying out rooting induction culture; and thus successfully obtaining root-containing tissue culture seedlings by the tissue culture propagation method of the invention, wherein the proliferation multiple is 86 times, the rooting rate is 82%, the average rooting number is 3.1, and the exercising seedling survival rate reaches 90%. The method can efficiently obtain the michelia compressa seedlings, and greatly meets the demand of markets.
Description
Technical field
The invention belongs to plant biotechnology field, be specifically related to a kind of Taiwan propagation method that (Michelia compressa) tissue is cultivated with a smile.
Background technology
Taiwan is (Michelia compressa) with a smile, belongs to Magnoliaceae (Magnoliaceae) Michelia (Michelia L.) plant.Evergreen megaphanerophyte, bark taupe, slightly smooth.Ye Quanyuan or wavy edge, keratin, leaf bud and bud have draped over one's shoulders russet fine hair.In Evergreen Broad-leaved Tree Species, the landscape effect that have a smile on one's face in Taiwan is outstanding, tree-like compactness, dryness is stronger, and tree crown is closely coniform, and side shoot is all upward to be stretched, draw close to trunk, intensive 1,2 year vimineous top, the formation dark green and dense tree crown of the four seasons be born in of dark green and glossiness blade.Spend petite simple and elegantly, breezy, gives out light fragrance.By expanding numerous popularization, Taiwan can become greening Jiangsu Province and even East China fast-growing, that ornamental value is higher completely with a smile and view and admire fine tree species.
Taiwan originates in Taiwan with a smile, is distributed in Taiwan and Japan.Central China finds without wild resource, and cultivation aspect only one place, Nanjing Zhong Shan botanical garden is introduced a fine variety and preserved 5 strains.Maternal quantity is extremely limited; the original producton location that have a smile on one's face in Taiwan is at the greatest differences of weather, soil and other biological condition; cause Taiwan to introduce a fine variety with a smile rear solid amount little; and its seedling exists reproduction speed slow; breeding cycle is long; percentage of seedgermination is lower; seedling is difficult for the defects such as survival; therefore, carry out Taiwan and have a smile on one's face the research of vegetative propagation technique, not only can effectively increase Tree Species Resources storage; lay material base for developing; and be conducive to Taiwan and have a smile on one's face the protections of seeds, cultivate about the tissue of Magnoliaceae Michelia plant, rarely have report both at home and abroad.Liu Fang etc. are the tissue cultivation that explant has been studied Michelia Yunnanensis by lateral bud, blade, draw method (the Michelia Yunnanensis tissue culture rapid propagating technology that meets approach breeding Michelia Yunnanensis group training seedling, Crop Diseases in Yunnan science and technology, the 2nd phase in 2011,12-13 page).The employing bearing tree stem with bud such as Zhu Bihua are that explant has been studied the pale reddish brown tissue cultivating and seedling technology of indefinite bud approach with a smile (pale reddish brown tissue culture technique research of having a smile on one's face, Agriculture of Anhui science, the 29th phase in 2009,14024-14027 page).Li Xue etc. are taking stem section stem apex as explant, carry out the research of the cultivation of michelia macclurei tissue and plant regeneration, shown that (tissue of michelia macclurei is cultivated and plant regeneration, plant physiology communication for inducing culture, strong seedling culture base and root media, the 6th phase in 2005,783 pages).When Zeng Songjun, Peng Xiaoming utilize sleeping bud and seed germination the epicotyl of seedling and hypocotyl for explant induction has gone out the callus of Michelia maudiae and indefinite bud, (tissue of Michelia maudiae is cultivated and Fast-propagation, tropical and subtropical plant journal, the 3rd phase in 2000,264-268 page); Chen Yunxia paper is had a smile on one's face fast breeding technique to Taiwan and is carried out primary research, but, it only obtains growth coefficient is 2.906, in low-down level, differ greatly with other woody plant group training effect, and its research all take root for processing and all do not have to take root, all there is no root induction success, therefore, successfully do not obtain tissue culture propagation band root seedling.
At present tissue culture propagation being had a smile on one's face in Taiwan still rested on compared with the primary stage, the invention provides a kind of Taiwan propagation method that tissue is cultivated with a smile, it is simple and quick, the rate of increase and growth rate are greatly improved, obtain containing root aseptic seedling, thereby can solve Taiwan with a smile in aspect demands such as urban landscaping, water source self-restraint, water and soil conservation, green landscape passage, ecological constructions.
Summary of the invention
The object of the invention is to disclose a kind of Taiwan propagation method that with a smile tissue is cultivated, expanded numerous Taiwan and have a smile on one's face clone plant technical support is provided for a large amount of.
In order to achieve the above object, the invention provides a kind of Taiwan propagation method that tissue is cultivated with a smile, comprise the steps:
(1) preparation of explant: March plant is had a smile on one's face in the Taiwan of intending gathering axillalry bud explant and carry out putting into hot-house culture after stump processing, explant plant is the 2 years raw Taiwan container seedling of growing directly from seeds with a smile, the major branch that prunes away at 5cm near the ground place March, and insert hot-house culture;
(2) explant is chosen and is sterilized: choose raw top slightly as explant then, after running water is rinsed well, carry out disinfection, sterilization method is for dipping in a little washing powder by its surface brush wash clean with hairbrush, then rinse 30 minutes with running water flowing water, dilute on the 84 thimerosal shaking tables of 50 times vibration sterilization 10 minutes, flowing water rinsed after 3 minutes, on aseptic operating platform with 75% ethanol disinfection 50-60 second, pour immediately 0.1% mercuric chloride sterilization 10 minutes into, finally, with sterile water washing 3 times, 3-5 minute vibrates at every turn;
(3) preparation of improvement WPM medium: use KNO
3200mg/L replaces the K in former WPM medium
2sO
4, adding in addition concentration of activated carbon is 1000mg/L, other components are all unchanged.(note: the present invention's all improvement WPM medium used are the method preparation)
(4) induction of indefinite bud: get the explant after sterilization in step (2), be cut into the long stem section of 0.8cm, every stem section is with an axillalry bud, be inoculated in the induction of carrying out indefinite bud in inducing culture, cultivation temperature is 20-26 DEG C, cultivates first in the dark 12 hours, then illumination cultivation 20-25 days: light application time is 10-16 hour/day, intensity of illumination is 1600-20001x, and described adventitious bud induction culture base is improvement WPM medium, and the hormonal readiness of interpolation is:
KT-301mg/L+ zeatin 0.8mg/L+6-BA0.1mg/L+GA
30.5mg/L;
(5) high-voltage electrostatic field HVEF processes, and utilizes high-voltage electrostatic field instrument, carries out electrostatic field processing, and intensity is 10KV/cm, 0.5 hour time;
(6) shoot proliferation: will access shoot proliferation medium after the indefinite bud cutting of processing through high-voltage electrostatic field, carry out subculture and cultivate 20-30 days, cultivation temperature is 20-26 DEG C, light application time is 10-16 hour/day, intensity of illumination is 1600-20001x, and shoot proliferation medium is for improvement WPM medium and add zeatin 0.2mg/L+KT-300.2mg/L+IBA0.1mg/L;
(7) culture of rootage: have a smile on one's face after unrooted test-tube plantlet cuts in the Taiwan that step (6) is obtained, every strain all has an indefinite bud, in access root media, carry out culture of rootage 20-30 days, cultivation temperature is 20-26 DEG C, light application time is 10-16 hour/day, intensity of illumination is 1600-2000Lx, and root media improvement WPM medium also adds IBA3.0mg/L;
(8) the domestication hardening of aseptic seedling: step (7) gained Taiwan is opened with the blake bottle bottle cap of root aseptic seedling with a smile, outdoor cultivation 2-3 days, then move in the matrix that adopts carbendazim sterilization, group training seedling top is used PVC film with holes to cover moisturizing, domestication container adopts 50 hole dishes, be placed on ventilating and cooling place and cultivate 25-30 days, cultivate and by the 15th day, PVC film is removed, use the sunshade net of index of refraction 75% instead; Described matrix components is the weight ratio red soil of 1: 1 and perlite;
In above-mentioned adventitious bud induction culture base, shoot proliferation medium, root media, all contain agar 6.0-7.0g/L, sucrose 20-35g/L, and pH value is 5.2-6.0.
The Taiwan of the present invention propagation method that tissue is cultivated with a smile has the following advantages:
1, the rear immigration of plant " stump " hot-house culture of explant material will be provided: first, because the nutrient of explant plant root accumulation is many, " stump " technology can promote plant trunk grow fast, highly to increase obviously, especially can impel axillalry bud full, be conducive to induce axillary bud sprouting in tissue cultivation.
Disinfecting of 2 explants, present inventor is through a large amount of creative works and factorial experiment, major tuneup operating parameter of the prior art, finding, is single clorox or mercuric chloride sterilization according to conventional disinfection way, has certain pollution rate, survival rate and brown rate are unsatisfactory, adopt the application's operating process, not only obtained desirable sterilization effect
Can also ensure the required explant number of induction cultivation.
| Group | Sterilization method | Pollution rate | Brown rate |
| 1 | 0.1% mercuric chloride sterilization 12 minutes | 11％ | 10％ |
| 2 | 84 thimerosal 5min; 0.1% mercuric chloride sterilization 10 minutes | 10％ | 8％ |
| 3 | " Amway " 30min; 84 thimerosal 5min; 0.1% mercuric chloride sterilization 10 minutes | 9％ | 15％ |
| 4 | 84 thimerosal 10min; 75% ethanol disinfection 50-60 second; 0.1% mercuric chloride sterilization 10 minutes | 5％ | 0 |
3 when cultivate on WPM medium, and explant indefinite bud is difficult for differentiating branch, quick-fried bud and not extending only on explant, and on MS medium, differentiation adventitious buds is leaf bud, to not effect of subculture increment.Poor growth in B5, indefinite bud is thin and weak, by after the improvement of WPM medium, has changed NPK ratio, uses KNO
3200mg/L replaces the K in former WPM medium
2sO
4, adding in addition concentration of activated carbon is 1000mg/L, other components are all unchanged, can significantly promote differentiation and the growth of indefinite bud.
Be below identical Hormone Conditions, different culture media screening segmentation scheme:
The screening of minimal medium
In 4 adventitious bud inducing growth cycles because of the restriction of damaged, nutrition condition, envirment factor with affect S-type growth curve, but the processing of electrostatic field has affected the motion of permeability and other polar molecule of film, absorption and the transhipment physical capacity of cell are improved, thereby can promote the growth of tissue, make subculture shortening period of delay, enter very soon exponential phase.Utilize electrostatic field processing, different intensity and ageing can produce different effects, if it is excessive to process electric-field intensity, or the processing time is long, can make plant cell come to harm even dead, and electric-field intensity is too small or the time is too short, can cause again treatment effect not obvious, present inventor show that through great many of experiments it is 10KV/cm that Taiwan is adopted to intensity with a smile, time, halfhour electrostatic field processing can significantly improve upgrowth situation, fresh weight increases obviously, growth rate is 110.7mg/d, division and the growth of cell are greatly promoted, shorten the time of cultured in vitro.
How 5 utilize plant tissue differentiation and regeneration plant for plant tissue culture area research, improve the breeding quantity of plant, that to study important technology emphasis be also technological difficulties to scientist, and hormone kind and the hormone concentration scope of application, type of culture medium is the most important thing especially, present inventor, through a large amount of creative works, filters out adventitious bud induction culture base, proliferated culture medium and the root media of having a smile on one's face in Taiwan and is respectively:
Improvement WPM medium+KT-301mg/L+ zeatin 0.8mg/L+6-BA0.1mg/L+GA
30.5mg/L;
Improvement WPM medium+zeatin 0.2mg/L+KT-300.2mg/L+IBA0.1mg/L;
Improvement WPM medium+IBA3.0mg/L
The WPM medium of improvement, adds appropriate active carbon, and the oozy poisonous secondary metabolites of absorption plant makes plant strain growth vigor keep vigorous, plays the effect of brownization of containment;
Adopt tissue culture propagation of the present invention, finally breeding multiple is 81-86 times; Wherein adopt shoot proliferation medium, propagation multiple is 8 times; Adopt inducing culture, adventitious bud induction frequency is 94%, adopts root media, and rooting rate is 82%, and the number of on average taking root is 3.1; Hardening survival rate reaches 90%.
Embodiment
(1) preparation of explant: in March, 2012 plant is had a smile on one's face in the Taiwan of intending gathering axillalry bud explant and carry out putting into hot-house culture after stump processing, explant plant is the 2 years raw Taiwan container seedling of growing directly from seeds with a smile, the major branch that prunes away at 5cm near the ground place March, and insert hot-house culture;
(2) explant is chosen and is sterilized: late June, choose raw top slightly as explant then, after running water is rinsed well, carry out disinfection, sterilization method is for dipping in a little washing powder by its surface brush wash clean with hairbrush, then rinse 30 minutes with running water flowing water, dilute on the 84 thimerosal shaking tables of 50 times vibration sterilization 10 minutes, flowing water rinsed after 3 minutes, on aseptic operating platform with 75% ethanol disinfection 50-60 second, pour immediately 0.1% mercuric chloride sterilization 10 minutes into, finally, with sterile water washing 3 times, 3-5 minute vibrates at every turn;
(3) preparation of improvement WPM medium: use KNO
3200mg/L replaces the K in former WPM medium
28O
4, adding in addition concentration of activated carbon is 1000mg/L, other components are all unchanged.
(4) induction of indefinite bud: get the explant after sterilization in step (2), be cut into the long stem section of 0.8cm, every stem section is with an axillalry bud, be inoculated in the induction of carrying out indefinite bud in inducing culture, cultivation temperature is 20-26 DEG C, cultivates first in the dark 12 hours, then illumination cultivation 20-25 days: light application time is 10-16 hour/day, intensity of illumination is 1600-20001x, and described adventitious bud induction culture base is improvement WPM medium, and the hormonal readiness of interpolation is:
KT-301mg/L+ zeatin 0.8mg/L+6-BA0.1mg/L+GA
30.5mg/L;
(5) high-voltage electrostatic field HVEF processes, and utilizes high-voltage electrostatic field instrument, carries out electrostatic field processing, and intensity is 10KV/cm, 0.5 hour time;
(6) shoot proliferation: will access shoot proliferation medium after the indefinite bud cutting of processing through high-voltage electrostatic field, carry out subculture and cultivate 20-30 days, cultivation temperature is 20-26 DEG C, light application time is 10-16 hour/day, intensity of illumination is 1600-20001x, and shoot proliferation medium is for improvement WPM medium and add zeatin 0.2mg/L+KT-300.2mg/L+IBA0.1mg/L;
(7) culture of rootage: have a smile on one's face after unrooted test-tube plantlet cuts in the Taiwan that step (6) is obtained, every strain all has an indefinite bud, in access root media, carry out culture of rootage 20-30 days, cultivation temperature is 20-26 DEG C, light application time is 10-16 hour/day, intensity of illumination is 1600-2000Lx, and root media improvement WPM medium also adds IBA3.0mg/L;
(8) the domestication hardening of aseptic seedling: step (7) gained Taiwan is opened with the blake bottle bottle cap of root aseptic seedling with a smile, outdoor cultivation 2-3 days, then move in the matrix that adopts carbendazim sterilization, group training seedling top is used PVC film with holes to cover moisturizing, domestication container adopts 50 hole dishes, be placed on ventilating and cooling place and cultivate 25-30 days, cultivate and by the 15th day, PVC film is removed, use the sunshade net of index of refraction 75% instead; Described matrix components is the weight ratio red soil of 1: 1 and perlite;
In above-mentioned adventitious bud induction culture base, shoot proliferation medium, root media, all contain agar 6.0g/L, sucrose 20g/L, and pH value is 5.2.
The tissue culture propagation that adopts above-mentioned Taiwan to have a smile on one's face, successfully obtains containing root group training seedling, and propagation multiple is 86 times; Wherein adopt shoot proliferation medium, propagation multiple is 8 times; Adopt inducing culture, adventitious bud induction frequency is 94%, adopts root media, and rooting rate is 82%, and the number of on average taking root is 3.1; Hardening survival rate reaches 90%, can obtain efficiently Taiwan and have a smile on one's face seedling, has greatly met the demand in market.
Although, above the present invention is described in detail with a general description of the specific embodiments, but on basis of the present invention, can make some modifications or improvements it, or seeds outside case study on implementation implement this method, this will be apparent to those skilled in the art.Therefore, these amendments of making without departing from theon the basis of the spirit of the present invention, the expansion of improvement or scope, all belongs to the scope of protection of present invention.
Claims (1)
1. a tissue culture propagation is had a smile on one's face in Taiwan, comprises the steps:
(1) preparation of explant: March plant is had a smile on one's face in Taiwan and carry out putting into hot-house culture after stump processing;
(2) explant is chosen and is sterilized: in June, choose raw top then and, slightly as explant, after running water is rinsed well, carry out disinfection;
(3) preparation of improvement WPM medium: use KNO
3200mg/L replaces the K in former WPM medium
2sO
4, adding in addition concentration of activated carbon is 1000mg/L, other components are all unchanged;
(4) induction of indefinite bud: get the explant after sterilization in step (2), be cut into the long stem section of 0.8cm, every stem section is with an axillalry bud, be inoculated in the induction of carrying out indefinite bud in adventitious bud induction culture base, cultivation temperature is 20-26 DEG C, first cultivate in the dark 12 hours, then illumination cultivation 20-25 days, light application time is 10-16 hour/day, intensity of illumination is 1600-2000lx, and described adventitious bud induction culture base is that the hormonal readiness of described improvement WPM medium and interpolation is:
KT-30lmg/L+ zeatin 0.8mg/L+6-BA0.1mg/L+GA
30.5mg/L;
(5) high-voltage electrostatic field HVEF processes: utilize high-voltage electrostatic field instrument, indefinite bud is carried out to electrostatic field processing, intensity is 10KV/cm, 0.5 hour time;
(6) shoot proliferation: by the indefinite bud access shoot proliferation medium of processing through high-voltage electrostatic field, carry out subculture and cultivate 20-30 days, cultivation temperature is 20-26 DEG C and obtains unrooted test-tube plantlet, light application time is 10-16 hour/day, intensity of illumination is 1600-2000lx, and shoot proliferation medium is described improvement WPM medium and adds zeatin 0.2mg/L+KT-300.2mg/L+IBA0.1mg/L;
(7) culture of rootage: the Taiwan that step (6) is obtained has a smile on one's face to carry out culture of rootage 20-30 days in unrooted test-tube plantlet access root media, cultivation temperature is 20-26 DEG C, light application time is 10-16 hour/day, intensity of illumination is 1600-2000Lx, and root media is described improvement WPM medium and adds IBA3.0mg/L;
(8) the domestication hardening of aseptic seedling;
The sterilization of described step (2) is: dip in a little washing powder by explant surface brush wash clean with hairbrush, then rinse 30 minutes with running water, subsequently with vibration sterilization on 84 thimerosal shaking tables of 50 times of dilutions 10 minutes, then flowing water rinsed after 3 minutes, on aseptic operating platform with 75% ethanol disinfection 50-60 second, pour immediately 0.1% mercuric chloride sterilization 10 minutes into, finally, with sterile water washing 3 times, 3-5 minute vibrates at every turn.
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Families Citing this family (6)
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| CN103718969B (en) * | 2014-01-28 | 2015-11-18 | 华南农业大学 | A kind of logical cultured in vitro regeneration plant method with a smile of poem beautiful jade |
| CN104126492B (en) * | 2014-08-06 | 2016-04-13 | 江苏省中国科学院植物研究所 | The in-vitro propagation method of a kind of middle mountain China fir kind 136 |
| CN105900842B (en) * | 2016-05-09 | 2017-12-08 | 中国林业科学研究院热带林业研究所 | A kind of Michelia macclurei tissue-culturing quick-propagation method for culturing seedlings |
| CN112314441A (en) * | 2020-12-26 | 2021-02-05 | 江苏省中国科学院植物研究所 | Tissue culture propagation method of cinnamomum japonicum |
| CN114600772B (en) * | 2022-03-23 | 2023-02-14 | 中国科学院昆明植物研究所 | Tissue culture method and rapid propagation method of michelia figo in remote mountains |
| CN117598204B (en) * | 2024-01-24 | 2024-03-22 | 西南林业大学 | Yunnan Hanxiao tissue culture and rapid propagation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887065A (en) * | 2006-07-21 | 2007-01-03 | 中国科学院昆明植物研究所 | Tissue culture propagation process of late spring michelia |
| CN1985578A (en) * | 2006-12-05 | 2007-06-27 | 吴月燕 | Tissue culture seedling producing process for lechang michelia and golden leaf michelia |
| CN102511399A (en) * | 2012-01-13 | 2012-06-27 | 南京金埔园林股份有限公司 | Acer mono maxim axillary bud path tissue culture method |
-
2013
- 2013-08-09 CN CN201310344980.2A patent/CN103392601B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1887065A (en) * | 2006-07-21 | 2007-01-03 | 中国科学院昆明植物研究所 | Tissue culture propagation process of late spring michelia |
| CN1985578A (en) * | 2006-12-05 | 2007-06-27 | 吴月燕 | Tissue culture seedling producing process for lechang michelia and golden leaf michelia |
| CN102511399A (en) * | 2012-01-13 | 2012-06-27 | 南京金埔园林股份有限公司 | Acer mono maxim axillary bud path tissue culture method |
Non-Patent Citations (2)
| Title |
|---|
| 木兰科7种植物的组织培养技术研究;王碧琴等;《江西农业大学学报》;20060430;第28卷(第2期);268-273 * |
| 王碧琴等.木兰科7种植物的组织培养技术研究.《江西农业大学学报》.2006,第28卷(第2期),268-273. |
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