CN103386149A - Net abdominal wall defect repairing material with sustain-released pain relieving effect and preparation method thereof - Google Patents

Net abdominal wall defect repairing material with sustain-released pain relieving effect and preparation method thereof Download PDF

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CN103386149A
CN103386149A CN2013102857325A CN201310285732A CN103386149A CN 103386149 A CN103386149 A CN 103386149A CN 2013102857325 A CN2013102857325 A CN 2013102857325A CN 201310285732 A CN201310285732 A CN 201310285732A CN 103386149 A CN103386149 A CN 103386149A
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mesh
abdominal wall
material
polypropylene
paraffin
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CN2013102857325A
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钟春燕
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钟春燕
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Abstract

The invention discloses a net abdominal wall defect repairing material with sustain-released pain relieving effect and a preparation method thereof, and relates to the field of medical instruments and manufacturing techniques of the medical instruments. The preparation method comprises following steps: selecting and activating bacterial strains, which can secrete bacterial cellulose, preparing the bacterial strains into seed mash, evenly dropping the seed mash on the surface of the polypropylene mesh, which has been subjected to a wax treatment, adding a fermentation medium, culturing for 1-3d, taking out the fermentation product, subjecting the fermentation product to wax elimination and purification treatments so as to obtain the net abdominal defect repairing material, soaking the repairing material in a polylactic acid-glycolic acid copolymer solution containing a little of bupivacaine compound, taking the reparing material out after 1 hour, drying so as to get a net abdominal wall reparing material with sustain-released pain relieving effect. The net abdominal wall defect repairing material with sustain-released pain relieving effect has excellent mechanical properties. After being implanted into a body, the net structure is beneficial for fiber textures to grow and penetrate through, thereby chronic inflammatory response and adhesion inside abdominal walls are avoided, and the post-operation pains are prominently relieved.

Description

具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法技术领域 Mesh having sustained analgesia abdominal wall defect repair material and method Technical Field

[0001] 本发明涉及医疗器械及其制备技术领域,特别涉及一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法。 [0001] The present invention relates to medical devices and their preparation technology, and particularly relates to a mesh having abdominal wall defects sustained analgesia material and its preparation method.

背景技术 Background technique

[0002] 腹壁缺损是支撑脏器的腹壁在某个部位出现断裂或者弱化,使腹腔脏器连同腹膜经腹壁薄弱处或孔隙向体表突出而形成局部肿块。 [0002] The abdominal wall defect is to support the organ or abdominal wall breaks at a weakened portion of the abdominal organs together with weakness in the abdominal wall peritoneum or surface porosity is formed to protrude local mass. 这种腹壁缺损各种年龄均可能发生,正常人腹壁上有一些薄弱的区域,如腹股沟管、股环;加上有的人腹壁某一部位发育有缺陷,如脐环闭锁不全、腹白线缺损等;还有手术切口、外伤造成的腹壁损伤;老年肌肉萎缩造成腹壁肌肉薄弱等都会造成腹壁缺损。 Such abdominal wall defects are likely to occur all ages, the normal human abdominal wall has some weak areas, such as the inguinal canal, femoral ring; plus some people have developed a certain part of the abdominal wall defects such as insufficiency navel ring, the linea alba and other defects; there are surgical incisions, trauma caused by abdominal trauma; elderly muscle atrophy caused by weak abdominal muscles, and this will result in abdominal wall defects. 腹壁缺损是不能自愈的,尽管它不会在短时间内恶化。 Abdominal wall defects can not heal, although it will not deteriorate in a short time. 早期或有轻度症状时,患者会感到疼痛,下腹坠胀,消化不良和腹泻等症状。 Early or mild symptoms, the patient will feel pain, abdominal distension, diarrhea, indigestion and other symptoms. 病情不断发展后,由于肿块坠入阴囊,会造成活动及行走不便。 After the development of the disease, due to the fall in the scrotum mass, activity and cause difficulties in walking. 严重者会发生嵌顿,如不及时处理,会造成肠坏死,甚至危机生命,因此要采取及时的治疗措施。 In severe cases, incarceration will happen, if not treated, can cause intestinal necrosis, or even life-threatening, so to take timely treatment measures.

[0003] 目前临床上较普遍的方法是采用“无张力修补术”(Tension-free Technique),使用腹壁修复材料填补缺损。 [0003] Current clinical more common approach is to use "tension-free repair" (Tension-free Technique), the abdominal wall repair material used to fill the defect. 聚丙烯腹壁修复材料是临床上使用最广且效果较好的一种高分子材料,它具有良好的生理惰性、生物相容性、优良的韧性和机械性能。 Abdominal wall repair material polypropylene is the most widely used, and a better clinical polymer material, which has good physiologically inert, biocompatible, excellent toughness and mechanical properties. 一般采用编织的方法将单丝聚丙烯纤维编织连接成网状结构。 General method braided monofilament woven polypropylene fibers is connected into a network structure. 根据临床使用和大量临床资料,显示这种聚丙烯腹壁修复材料植入体内后会引起慢性炎症反应和腹壁内的粘连。 The large number of clinical data and clinical use, show such a polypropylene material after implantation in vivo repair of abdominal wall can cause chronic inflammation in the abdominal wall and the adhesions. 为了减少其负性并发症效应,轻质聚丙烯网片相应出现,这种轻质聚丙烯网片减少了聚丙烯的用量并增大了网状结构的网孔口径。 In order to reduce the negative effects of complications, the respective light polypropylene mesh occurs, light polypropylene mesh which reduce the amount of the polypropylene and increasing the diameter of the mesh network structure. 临床研究证实轻质聚丙烯网片具有以下的优势:更柔软,更耐受弯曲;刺激纤维组织增生作用更明显,网孔孔径增大,更利于纤维组织成长穿过,更被结缔组织浸润,能够早期与组织嵌合;更耐受感染,即使在脓性感染伤口中肉芽组织仍然可以增殖于网孔内,不引起网片腐蚀形成。 Clinical studies of light polypropylene mesh has the following advantages: a more flexible, more resistant to bending; stimulating proliferation of fibrous tissue more obvious effect, aperture size increases, more conducive to the growth of fibrous tissue through more invasive connective tissue, early tissue can be fitted; more resistant to infection, even in the granulation tissue purulent infection in the wound may still be within the cell proliferation without causing web etching. 然而,聚丙烯的减少并不能从根本上解决由材料本身引起的炎症反应。 However, reduction of the polypropylene does not fundamentally solve the inflammatory response caused by the material itself. 在用于腹壁全层缺损修补时,聚丙烯网片与内脏器官直接接触可引起炎症反应、腹腔粘连,甚至可能侵蚀肠壁,引起肠瘘。 When a full-thickness abdominal wall defect repair, polypropylene mesh and organ contact may cause inflammation, abdominal adhesions, erosion of the intestinal wall and may even cause intestinal fistula. 另外,大量临床报告显示,患者在腹壁缺损修复手术后有强烈的术后疼痛,尤其是幼儿患者更加常见。 In addition, a large number of clinical reports show that patients have a strong postoperative pain after surgery to repair abdominal wall defects, especially the more common pediatric patients.

[0004] 因此,制备一种具有缓释镇痛效果的腹壁缺损修复材料既可以保证柔顺性能、力学性能和网孔结构,又可以提高修复材料与组织的生物相容性避免炎症反应,缓解术后疼痛,将具有巨大的应用价值。 Abdominal wall defect repair material [0004] Thus, the preparation having a sustained analgesic effect can guarantee compliant properties, mechanical properties and the mesh structure, but also can improve the biocompatibility and tissue repair material to avoid inflammation, remission surgery after the pain, it will have great value.

[0005] 本专利首先采用细菌发酵法,将细菌纤维素包裹在构成聚丙烯网片的聚丙烯纤维上,然后通过浸溃法将载有缓释镇痛药物的聚乳酸-羟基乙酸共聚物颗粒固定在细菌纤维素的三维纳米纤维网络的内部及表面,制备出一种具有缓释镇痛效果的网状腹壁缺损修复材料。 [0005] Firstly, the present patent bacterial fermentation, the bacterial cellulose constituting the polypropylene is wrapped around the web of polypropylene fibers, and then the sustained-release analgesics containing impregnation method by polylactic acid - glycolic acid copolymer particles fixed to the inside surface of the three-dimensional network of fibers and bacterial cellulose prepared having a mesh abdominal wall defects sustained analgesia material.

发明内容 SUMMARY

[0006] 本发明公开了一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法。 [0006] The present invention discloses a mesh having abdominal wall defects analgesia release material and its preparation method. 涉及一种医疗器械及其制备技术领域。 It relates to a technical field of medical devices and their preparation. 本发明工艺简单,操作方便,制备的一种具有缓释镇痛效果的网状腹壁缺损修复材料力学性能优良,缓释镇痛效果显著,植入体内后网状结构有利于纤维组织生长穿过,可避免慢性炎症反应和腹壁内的粘连,并可以显著缓解术后疼痛。 The present process is simple, easy to operate, the abdominal wall having a mesh release analgesia produced defect excellent mechanical properties, remarkable analgesic effect release, in vivo after implantation of the mesh structure is conducive to the growth of fibrous tissue through can be avoided in the abdominal adhesions inflammation and chronic, and can significantly relieve postoperative pain.

[0007] 本发明公开了一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法。 [0007] The present invention discloses a mesh having abdominal wall defects analgesia release material and its preparation method. 包括:选取能分泌细菌纤维素的菌株活化制备成种子醪液,将种子醪液均匀滴加在经石蜡处理的聚丙烯网片表面,加入发酵培养基培养I〜3d后取出发酵产物。 Comprising: preparing activated to seed mash select strains secreting bacterial cellulose, the seed was uniformly dropped mash web surface treated polypropylene paraffin, added to the fermentation culture medium after fermentation product taken I~3d. 发酵产物经石蜡脱除、纯化处理得到网状腹壁缺损修复材料。 Paraffin fermentation product removal, purification treatment of abdominal wall defects resulting mesh material. 将修复材料浸泡在含有少量布比卡因类化合物的聚乳酸-羟基乙酸共聚物溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The repair material is immersed in the polylactic acid-based compound containing a small amount of bupivacaine - glycolic acid copolymer solution, taken out after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material.

[0008] 作为优选的技术方案: [0008] As a preferred technical solution:

其中,如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的种子醪液,所含的菌株浓度为60〜95% ;所述的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片的表面,5〜IOmin后加入发酵培养基,发酵培养温度为25〜35°C,时间为I〜3d。 Wherein, the mesh having abdominal sustained analgesia above defects material and method, the seed mash, strain contained in a concentration of 60~95%; the seed mash a lattice uniformly dropped paraffin surface treated polypropylene mesh, added to the fermentation medium was 5~IOmin, fermentation temperature is 25~35 ° C, time I~3d.

[0009] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的聚丙烯网片为聚丙烯纤维编织成型的具有网孔结构的聚丙烯网片,厚度为0.1〜0.4mm,密度为20〜45g/m2,网片的网孔口径为4.0〜10.0mm2。 [0009] abdominal wall having a mesh release analgesic effect described above defects material and method, the polypropylene woven mesh formed of polypropylene fibers having a polypropylene mesh mesh structure thickness 0.1~0.4mm, density 20~45g / m2, the mesh of the mesh diameter of 4.0~10.0mm2.

[0010] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的经石蜡处理的聚丙烯网片,处理方法为:聚丙烯网片浸入石蜡溶液中少许时间后取出,待石蜡凝固后将其浸入正己烷中5〜lOmin。 [0010] abdominal mesh having sustained analgesia defect repair material as described above and its preparation method, the polypropylene mesh paraffin processing, a processing method of: a polypropylene mesh was immersed in a solution of paraffin after some time in the extraction, will be immersed in n-hexane solidified paraffin 5~lOmin. 一般情况下,菌种在聚丙烯网片上生长时,会进入网片的网孔结构中,形成无孔的片层。 In general, the bacteria when grown on a polypropylene mesh, will mesh sheet into the network structure, formed of non-porous sheet. 在本发明中,为了维持网孔结构,首先采用石蜡处理占据网孔空间,待细菌纤维素在聚丙烯纤维上形成包覆后再将石蜡除去。 In the present invention, in order to maintain the mesh structure, paraffin first processing space occupied by the mesh, the bacterial cellulose is formed to be coated on the polypropylene fiber and then to remove the paraffin. 石蜡处理后,需将聚丙烯网片浸入正己烷一段时间,`目的是为了除去聚丙烯纤维表面及内部的石蜡。 Paraffin treatment, polypropylene mesh was immersed in hexane for some time need to be, 'purpose is to remove the paraffin and the internal surface of the polypropylene fibers. 在发酵培养过程中,菌株可以在聚丙烯纤维表面及内部分泌细菌纤维素,并最终使细菌纤维素完全包裹聚丙烯纤维。 In the fermentation process, the strains can be secreted bacterial cellulose polypropylene fiber surface and the inside, and ultimately bacterial cellulose completely surrounded polypropylene fibers.

[0011] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的石蜡脱除方法为:在50〜70°C的温度下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡5〜IOmin。 [0011] abdominal mesh having sustained analgesia defect repair material as described above and its preparation method, the paraffin removal process as follows: at a temperature of 50~70 ° C, the fermentation product, respectively distilled water, immersed in isopropanol and ethanol 5~IOmin.

[0012] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的布比卡因类化合物可以是:布比卡因、盐酸布比卡因、盐酸左布比卡因或罗呱卡因,以及它们的衍生物。 [0012] abdominal wall as described above having a mesh sustained analgesia defect repair material and its preparation method, the compound may be bupivacaine: bupivacaine, bupivacaine hydrochloride, left ropivacaine or bupivacaine hydrochloride, and derivatives thereof.

[0013] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的聚乳酸-羟基乙酸共聚物溶液为:重量百分比为3〜5%的聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,所述的布比卡因类化合物占聚乳酸-羟基乙酸共聚物溶液的重量百分数为15〜25%。 [0013] abdominal mesh having sustained analgesia defect repair material as described above and a preparation method of the polylactic acid - glycolic acid copolymer solution is: 3 to 5% by weight of polylactic acid - glycolic acid copolymer was dissolved in chloroform, said compound accounting for bupivacaine polylactic - glycolic acid copolymer solution weight percentage of 15~25%. 目前,临床上为了缓解患者腹壁缺损修复手术后的疼痛,常采用在局部麻醉的手术部位皮下渗透镇痛药物或口服镇痛药物的方法,但这些方法都很难在术后24小时内提供持续有效的缓痛效果。 Currently, clinically for pain relief after surgery the patient's abdominal wall defects, the surgical site is often used in analgesia or local anesthesia methods subcutaneous osmotic oral analgesic, but these methods are difficult to provide continuously within 24 hours after surgery effective pain relief effect. 有研究表明,采用输液的形式将布比卡因类的镇痛药物持续释放到手术部位,可以有效缓解腹壁缺损修复手术后的疼痛。 Studies have shown that the form of the analgesic bupivacaine infusion type sustained release to a surgical site, can effectively relieve pain after surgery to repair abdominal wall defects. 在腹壁缺损修复手术中,常采用修复材料对缺损部位进行填补,本发明中缓释镇痛载药体系与腹壁缺损修复材料结合制备具有缓释镇痛效果的腹壁缺损修复材料将有效缓解手术后的疼痛。 After surgery in the abdominal wall defects, the repair material often used to fill the defect site for the present invention, the analgesic drug release system and abdominal wall defect repair material in combination with a sustained release preparation of the abdominal wall defect repair material analgesia will effectively alleviate surgery pain. 通过控制聚乳酸-羟基乙酸共聚物溶液中聚乳酸-羟基乙酸共聚物以及布比卡因类化合物的用量,调节该缓释镇痛载药体系在使用中24h内达到峰值,并在64h内持续释放镇痛药物。 By controlling the polylactic acid - glycolic acid copolymer solution of polylactic acid - glycolic acid copolymer and the amount of bupivacaine compounds, analgesic drug release regulating the system reached the peak in use, 24h, and 64h in the continued the release of analgesic drugs.

[0014] 如上所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料,其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为3.5〜9.0_2。 [0014] abdominal wall having a mesh release analgesic effect described above material and method for defect repair, the mesh having abdominal wall defects analgesia release material, wherein the bacterial cellulose uniformly wrapped in the polypropylene constituting the polypropylene fiber mesh, a mesh diameter of 3.5~9.0_2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0015] 与现有技术相比,本发明的有益效果是:采用石蜡处理、原位发酵法,将细菌纤维素包裹在构成聚丙烯网片的聚丙烯纤维上,并通过浸溃法将载有缓释镇痛药物的聚乳酸-羟基乙酸共聚物颗粒固定在细菌纤维素的三维纳米纤维网络的内部及表面,制备出一种具有缓释镇痛效果的网状腹壁缺损修复材料。 [0015] Compared with the prior art, the beneficial effects of the present invention are: paraffin treatment, in situ fermentation, the bacterial cellulose constituting wrapped in polypropylene mesh polypropylene fibers, and a carrier by impregnation method analgesic with a sustained polylactic acid - glycolic acid copolymer particles and the inner surface of the fixed three-dimensional network of fibers bacterial cellulose prepared having a mesh abdominal wall defects sustained analgesia material. 本发明工艺简单,操作方便,制备的一种具有缓释镇痛效果的网状腹壁缺损修复材料力学性能优良,缓释镇痛效果显著,植入体内后网状结构有利于纤维组织生长穿过,可避免慢性炎症反应和腹壁内的粘连。 The present process is simple, easy to operate, the abdominal wall having a mesh release analgesia produced defect excellent mechanical properties, remarkable analgesic effect release, in vivo after implantation of the mesh structure is conducive to the growth of fibrous tissue through , a chronic inflammatory response can be avoided in the abdominal wall and the adhesions.

具体实施方式 Detailed ways

[0016] 下面结合具体实施例,进一步阐述本发明。 [0016] The following embodiments with reference to specific embodiments, further illustrate the present invention. 应理解,这些实施例仅用于说明本发明而不用于限制本发明的范围。 It should be understood that these embodiments are illustrative only and the present invention is not intended to limit the scope of the invention. 此外应理解,在阅读了本发明讲授的内容之后,本领域技术人员可以对本发明作各种改动或修改,这些等价形式同样落于本申请所附权利要求书所限定的范围。 Furthermore, it should be understood that, after reading the teachings of the present invention, those skilled in the art that various changes or modifications may be made to the present invention, and these equivalents also fall within the scope of the appended claims of the present application as defined.

[0017] 实施例1: [0017] Example 1:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷5min,然后取出。 Immersing the polypropylene mesh was sterilized paraffin, wait removed, will be dipped in n-hexane solidified paraffin 5min, and then removed. 其中具有网孔结构的聚丙烯网片厚度为0.4mm,密度为20g/m2,网片的网孔口径为10.0mm20将能分泌细菌纤维素的木醋杆菌活化制备成种子醪液,然后将菌株浓度为60%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.4mm, a density of 20g / m2, the mesh of the mesh diameter of the 10.0mm20 secreting bacterial cellulose prepared as Acetobacter xylinum activated seed mash, and then the strain a concentration of 60% of the seeds in a lattice mash uniformly dropped polypropylene mesh paraffin-treated surface. 滴加种子醪液5min后加入发酵培养基。 Seed mash dropwise added to the fermentation medium after 5min. 发酵培养温度为25°C,时间为3d。 Fermentation temperature was 25 ° C, time is 3d.

[0018] 在50°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡lOmin。 [0018] at 50 ° C, respectively, the fermentation product in distilled water, isopropyl alcohol and anhydrous ethanol for lOmin. 然后在重量百分比为3%的NaOH水溶液中,80°C下加热6h。 Then aqueous NaOH 3% by weight, the heated 80 ° C for 6h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基得到网状腹壁缺损修复材料。 To remove cell adhesion proteins and the mesh obtained residue abdominal wall defect repair material in the cellulose film medium.

[0019] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为3%的溶液。 [0019] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer 3% by weight of the solution. 在溶液中加入布比卡因,其中布比卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为15%。 The solution was added bupivacaine, bupivacaine accounting polylactic wherein - the weight percent glycolic acid solution 15%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为9.0mm2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 9.0mm2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0020] 实施例2: [0020] Example 2:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷中6min。 Immersing the polypropylene mesh was sterilized paraffin, wait removed after solidification of the paraffin to be immersed in n-hexane to 6min. 其中具有网孔结构的聚丙烯网片厚度为0.3mm,密度为25g/m2,网片的网孔口径为 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.3mm, a density of 25g / m2, the mesh of the mesh diameter of

9.0mm2。 9.0mm2. 将能分泌细菌纤维素的根瘤菌属活化制备成种子醪液,然后将菌株浓度为95%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 Rhizobium genus secrete bacterial cellulose prepared as activated seed mash, and then the strain concentration of 95% of the seeds in a lattice mash uniformly dropped polypropylene mesh paraffin-treated surface. 滴加种子醪液6min后加入发酵培养基。 Seed medium was dropwise added to the fermentation mash 6min. 发酵培养温度为26°C,时间为2d。 Fermentation temperature was 26 ° C, time 2d.

[0021] 在50°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡9min。 [0021] at 50 ° C, respectively, the fermentation product in distilled water, isopropyl alcohol and anhydrous ethanol for 9min. 然后在重量百分比为1%的NaOH水溶液中,100°C下加热6h。 Then 1% by weight NaOH aqueous solution, was heated 100 ° C for 6h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基得到网状腹壁缺损修复材料。 To remove cell adhesion proteins and the mesh obtained residue abdominal wall defect repair material in the cellulose film medium.

[0022] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为4%的溶液。 [0022] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer, 4% by weight of the solution. 在溶液中加入布比卡因,其中布比卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为25%。 The solution was added bupivacaine, bupivacaine accounting polylactic wherein - the weight percent glycolic acid solution was 25%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为8.5mm2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 8.5mm2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0023] 实施例3: [0023] Example 3:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷中7min。 Immersing the polypropylene mesh was sterilized paraffin, wait removed after solidification of the paraffin to be immersed in n-hexane to 7min. 其中具有网孔结构的聚丙烯网片厚度为0.2mm,密度为30g/m2,网片的网孔口径为 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.2mm, a density of 30g / m2, the mesh of the mesh diameter of

8.0mm20将能分泌细菌纤维素的八叠球菌属活化制备成种子醪液,然后将菌株浓度为80%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 The bacteria can secrete 8.0mm20 cellulose Sarcina activated seed mash prepared, and then the strain concentration of 80% of the seeds in a lattice mash uniformly dropped polypropylene mesh paraffin-treated surface. 滴加种子醪液7min后加入发酵培养基。 Seed mash dropwise added to the fermentation medium after 7min. 发酵培养温度为28°C,时间为3d。 Fermentation temperature was 28 ° C, time is 3d.

[0024] 在60°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡8min。 [0024] at 60 ° C, respectively, the fermentation product in distilled water, isopropyl alcohol and anhydrous ethanol for 8min. 然后在重量百分比为5%的NaOH水溶液中,60°C下加热5h。 Then aqueous NaOH 5% by weight, the heated 60 ° C for 5h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基得到网状腹壁缺损修复材料。 To remove cell adhesion proteins and the mesh obtained residue abdominal wall defect repair material in the cellulose film medium.

[0025] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为5%的溶液。 [0025] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer is 5% by weight of the solution. 在溶液中加入盐酸布比卡因,其中盐酸布比卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为20%。 Hydrochloric acid solution was added bupivacaine, bupivacaine hydrochloride which accounted polylactic - weight percent glycolic acid solution was 20%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为7.0_2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 7.0_2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0026] 实施例4: [0026] Example 4:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷中8min。 Immersing the polypropylene mesh was sterilized paraffin, wait removed after solidification of the paraffin to be immersed in n-hexane to 8min. 其中具有网孔结构的聚丙烯网片厚度为0.1mm,密度为45g/m2,网片的网孔口径为 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.1mm, a density of 45g / m2, the mesh of the mesh diameter of

4.0mm20将能分泌细菌纤维素的无色杆菌属活化制备成种子醪液,然后将菌株浓度为70%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 The bacteria can secrete 4.0mm20 cellulose Achromobacter activated seed mash prepared, and then the concentration of 70% strain seed mash in a lattice uniformly dropwise polypropylene mesh paraffin-treated surface. 滴加种子醪液8min后加入发酵培养基。 Seed mash dropwise added to the fermentation medium after 8min. 发酵培养温度为30°C,时间为Id。 Fermentation temperature was 30 ° C, the time to Id.

[0027] 在60°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡7min。 [0027] at 60 ° C, respectively, the fermentation product in distilled water, isopropyl alcohol and anhydrous ethanol for 7min. 然后在重量百分比为8%的NaOH水溶液中,30°C下加热3h。 Then aqueous NaOH solution of 8% by weight, the heat at 30 ° C 3h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基。 To remove the residual bacterial protein in the medium and adhered to the cellulose film. 得到网状腹壁缺损修复材料。 Repairing abdominal wall defects resulting mesh material.

[0028] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为3%的溶液。 [0028] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer 3% by weight of the solution. 在溶液中加入盐酸左布比卡因,其中盐酸左布比卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为22%。 Left bupivacaine hydrochloride was added in the solution, wherein the left bupivacaine hydrochloride accounting polylactic - weight percent glycolic acid solution was 22%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为3.5_2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 3.5_2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0029] 实施例5: [0029] Example 5:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷中9min。 Immersing the polypropylene mesh was sterilized paraffin, wait removed after solidification of the paraffin to be immersed in n-hexane to 9min. 其中具有网孔结构的聚丙烯网片厚度为0.2mm,密度为40g/m2,网片的网孔口径为 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.2mm, a density of 40g / m2, the mesh of the mesh diameter of

5.0mm2。 5.0mm2. 将能分泌细菌纤维素的气杆菌属活化制备成种子醪液,然后将菌株浓度为85%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 The gas can secrete bacterial cellulose genus activated seed mash prepared, and then the strain was 85% in concentration in dot seed mash uniformly dropped polypropylene mesh paraffin-treated surface. 滴加种子醪液9min后加入发酵培养基。 Seed mash dropwise added to the fermentation medium after 9min. 发酵培养温度为35°C,时间为2d。 Fermentation temperature was 35 ° C, time 2d.

[0030] 在70°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡6min。 [0030] at 70 ° C, 6min fermentation products were immersed in distilled water, absolute ethanol and isopropanol. 然后在重量百分比为6%的NaOH水溶液中,70°C下加热4h。 Then aqueous NaOH 6% by weight, the heated 70 ° C for 4h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基。 To remove the residual bacterial protein in the medium and adhered to the cellulose film. 得到网状腹壁缺损修复材料。 Repairing abdominal wall defects resulting mesh material.

[0031] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为4%的溶液。 [0031] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer, 4% by weight of the solution. 在溶液中加入罗呱卡因,其中罗呱卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为18%。 Jia Ruluo quack tetracaine in solution, wherein ropivacaine accounting polylactic - weight percent glycolic acid solution 18%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为4.0mm2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 4.0mm2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

[0032] 实施例6: [0032] Example 6:

将经灭菌处理的聚丙烯网片浸入石蜡溶液,稍候取出,待石蜡凝固后将其浸入正己烷中lOmin。 Immersing the polypropylene mesh was sterilized paraffin, wait removed after solidification of the paraffin to be immersed in n-hexane lOmin. 其中具有网孔结构的聚丙烯网片厚度为0.3mm,密度为35g/m2,网片的网孔口径为7.0mm2。 Wherein the polypropylene mesh having a mesh structure having a thickness of 0.3mm, a density of 35g / m2, the mesh of the mesh diameter of 7.0mm2. 将能分泌细菌纤维素的产碱菌属与固氮菌属混合活化制备成种子醪液,然后将菌株浓度为65%的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片表面。 The bacteria can secrete cellulose Alcaligenes mixed with activated Azotobacter prepared seed mash, and then the strain concentration of 65% of the seeds in a lattice mash uniformly treated dropwise paraffin polypropylene mesh surface. 滴加种子醪液IOmin后加入发酵培养基。 Seed medium was dropwise added to the fermentation mash IOmin. 发酵培养温度为32°C,时间为3d。 Fermentation temperature was 32 ° C, time is 3d.

[0033] 在70°C下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡5min。 [0033] at 70 ° C, 5min fermentation products were immersed in distilled water, absolute ethanol and isopropanol. 然后在重量百分比为7%的NaOH水溶液中,50°C下加热3h。 Then aqueous NaOH of 7% by weight, the heated 50 ° C for 3h. 再用水反复冲洗至中性。 And then repeatedly washed with water until neutral. 以除去菌体蛋白和粘附在纤维素膜上的残余培养基。 To remove the residual bacterial protein in the medium and adhered to the cellulose film. 得到网状腹壁缺损修复材料。 Repairing abdominal wall defects resulting mesh material.

[0034] 将聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,得到聚乳酸-羟基乙酸共聚物重量百分比为5%的溶液。 [0034] The polylactic acid - glycolic acid copolymer was dissolved in chloroform, to give a polylactic acid - glycolic acid copolymer is 5% by weight of the solution. 在溶液中加入布比卡因,其中布比卡因占聚乳酸-羟基乙酸共聚物溶液的重量百分数为23%。 The solution was added bupivacaine, bupivacaine accounting polylactic wherein - the weight percent glycolic acid solution 23%. 将网状腹壁缺损修复材料浸泡在溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 The abdominal wall defect repair material mesh soaked in the solution, removed after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material. 其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为5.0mm2。 Wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 5.0mm2. 在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 Distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.

Claims (8)

1.一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,包括:选取能分泌细菌纤维素的菌株活化制备成种子醪液,将种子醪液均匀滴加在经石蜡处理的聚丙烯网片表面,加入发酵培养基培养I〜3d后取出发酵产物;发酵产物经石蜡脱除、纯化处理得到网状腹壁缺损修复材料;将修复材料浸泡在含有少量布比卡因类化合物的聚乳酸-羟基乙酸共聚物溶液中,Ih后取出,干燥得到一种具有缓释镇痛效果的网状腹壁缺损修复材料。 A mesh having a sustained abdominal analgesia defect repair material and its preparation method, comprising: preparing activated to seed mash select strains secreting bacterial cellulose, the seed mash was uniformly treated dropwise paraffin a polypropylene mesh surface, added to the fermentation culture medium was removed after fermentation product I~3d; fermentation product paraffin removal, purification treatment of abdominal wall defects resulting mesh material; soaked in a small amount of repair material compound containing bupivacaine polylactic acid - glycolic acid copolymer solution, taken out after Ih, and dried to obtain a mesh having abdominal wall defects sustained analgesia material.
2.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的种子醪液,所含的菌株浓度为60〜95% ;所述的种子醪液以点阵方式均匀滴加在经石蜡处理的聚丙烯网片的表面,5〜IOmin后加入发酵培养基,发酵培养温度为25〜35°C,时间为I〜3d。 2. mesh having sustained abdominal analgesia according to claim 1 defect repair material and method, wherein: said seed mash, strain contained in a concentration of 60~95% ; seed mash according to a lattice uniformly dropped paraffin surface treated polypropylene mesh, added to the fermentation medium was 5~IOmin, fermentation temperature is 25~35 ° C, time I~3d .
3.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的聚丙烯网片为聚丙烯纤维编织成型的具有网孔结构的聚丙烯网片,厚度为0.1〜0.4mm,密度为20〜45g/m2,网片的网孔口径为4.0〜10.0mm2。 One of the mesh 3. The abdominal wall as claimed in claim 1, having a sustained release analgesic effect of defect material and method, wherein: the polypropylene fiber woven polypropylene mesh having a mesh formed polypropylene mesh structure, a thickness of 0.1~0.4mm, density 20~45g / m2, the mesh of the mesh diameter of 4.0~10.0mm2.
4.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的经石蜡处理的聚丙烯网片,处理方法为:聚丙烯网片浸入石蜡溶液中少许时间后取出,待石蜡凝固后将其浸入正己烷中5〜lOmin。 4. one of the claim 1, having a mesh sustained analgesia abdominal wall defect repair material and its preparation method, wherein: said polypropylene mesh paraffin processing, a processing method of: poly propylene web after immersion in paraffin solution taken some time, which will be immersed in n-hexane solidified paraffin 5~lOmin.
5.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的石蜡脱除方法为:在50〜70°C的温度下,发酵产物分别在蒸馏水、异丙醇和无水乙醇中浸泡5 〜lOmin。 A temperature of 50~70 ° C: as claimed in claim 1, one of the mesh having sustained analgesia abdominal wall defect repair material and its preparation method, characterized in that: said removal process is paraffin wax lower, respectively, the fermentation product in distilled water, immersed in isopropanol and ethanol 5 ~lOmin.
6.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的布比卡因类化合物可以是:布比卡因、盐酸布比卡因、盐酸左布比卡因或罗呱卡因,以及它们的衍生物。 6. one of the claim 1, having a mesh sustained analgesia abdominal wall defect repair material and its preparation method, wherein: the compound may be bupivacaine: bupivacaine, bupivacaine hydrochloride, bupivacaine hydrochloride or left ropivacaine, and derivatives thereof.
7.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的聚乳酸-羟基乙酸共聚物溶液为:重量百分比为3〜5%的聚乳酸-羟基乙酸共聚物溶解于三氯甲烷中,所述的布比卡因类化合物占聚乳酸-羟基乙酸共聚物溶液的重量百分数为15〜25%。 7. The one of the claim 1 having a mesh abdominal wall defects sustained analgesia material and its preparation method, wherein: the polylactic acid - glycolic acid copolymer solution is: weight percentage 3 ~ 5% polylactic acid - glycolic acid copolymer was dissolved in chloroform, said compound accounting for bupivacaine polylactic - glycolic acid copolymer solution weight percentage of 15~25%.
8.如权利要求1所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料及其制备方法,其特征是:所述的一种具有缓释镇痛效果的网状腹壁缺损修复材料,其中细菌纤维素均匀包裹在构成聚丙烯网片的聚丙烯纤维上,网孔口径为3.5〜9.0_2,在细菌纤维素网络内部及表面分布着载有布比卡因类化合物的聚乳酸-羟基乙酸共聚物颗粒。 8. The one claim 1 having a mesh abdominal wall defects sustained analgesia material and its preparation method, wherein: said web having a release abdominal wall defect repair analgesia material, wherein the bacterial cellulose wrapped uniformly in the composition of the polypropylene fiber polypropylene mesh, a mesh diameter of 3.5~9.0_2, the distribution of bupivacaine contained in the polylactic acid-based compound BC network and the internal surface - glycolic acid copolymer particles.
CN2013102857325A 2013-07-09 2013-07-09 Net abdominal wall defect repairing material with sustain-released pain relieving effect and preparation method thereof CN103386149A (en)

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EP0797962A2 (en) * 1996-03-26 1997-10-01 ETHICON GmbH & Co. KG Areal implant
WO2009019685A2 (en) * 2007-08-03 2009-02-12 Nicast Ltd. Fibrous surgically implantable mesh
CN101810883A (en) * 2009-12-25 2010-08-25 上海太亨科贸有限公司 Bio-derived material with high tissue compatibility and long acting anti-infection as well as preparation method and application thereof
CN102316823A (en) * 2009-02-11 2012-01-11 新加坡南洋理工大学 Multi-layered surgical prosthesis
CN103041444A (en) * 2012-12-31 2013-04-17 钟春燕 Abdominal wall defect repair material and preparation method thereof
CN103055351A (en) * 2013-01-16 2013-04-24 海南光宇生物科技有限公司 Net abdominal wall defect repairing material and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0797962A2 (en) * 1996-03-26 1997-10-01 ETHICON GmbH & Co. KG Areal implant
WO2009019685A2 (en) * 2007-08-03 2009-02-12 Nicast Ltd. Fibrous surgically implantable mesh
CN102316823A (en) * 2009-02-11 2012-01-11 新加坡南洋理工大学 Multi-layered surgical prosthesis
CN101810883A (en) * 2009-12-25 2010-08-25 上海太亨科贸有限公司 Bio-derived material with high tissue compatibility and long acting anti-infection as well as preparation method and application thereof
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