CN103372107A - Chinese herbal microecological preparation for preventing and treating chronic obstructive pulmonary disease - Google Patents
Chinese herbal microecological preparation for preventing and treating chronic obstructive pulmonary disease Download PDFInfo
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- CN103372107A CN103372107A CN 201210138691 CN201210138691A CN103372107A CN 103372107 A CN103372107 A CN 103372107A CN 201210138691 CN201210138691 CN 201210138691 CN 201210138691 A CN201210138691 A CN 201210138691A CN 103372107 A CN103372107 A CN 103372107A
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Abstract
The invention belongs to the technical field of Chinese herbal medicines and particularly relates to a Chinese herbal microecological preparation for preventing and treating the chronic obstructive pulmonary disease. According to the technical scheme, a process for preparing the Chinese herbal microecological preparation comprises the following steps of: preparing a mother solution of the Chinese herbal microecological preparation, preparing a Chinese herbal extracting solution, inoculating the filtered Chinese herbal extracting solution against a mother solution of black-tea fungi or compound bacterial-cellulose producing acetic bacteria and compound yeast bacteria to obtain the Chinese herbal microecological preparation, fermenting the Chinese herbal microecological preparation, carrying out freeze drying on the fully fermented Chinese herbal microecological preparation at low temperature to prepare the bacterial powder of the Chinese herbal microecological preparation, and packaging the bacterial powder of the Chinese herbal microecological preparation in vacuum for long-term storage, wherein the Chinese herbal microecological preparation can be used for preventing and treating the chronic obstructive pulmonary disease. The Chinese herbal microecological preparation is non-toxic and can be taken orally and sprayed to prevent and treat the chronic obstructive pulmonary disease in the digestive tract and the respiratory tract simultaneously. The Chinese herbal microecological preparation can be degraded and adsorbed in the digestive tract to remove dust and other toxic particles in the lung. The Chinese herbal microecological preparation has a good effect of comprehensively preventing and treating the chronic obstructive pulmonary disease.
Description
One, technical field
The invention belongs to biological technical field, particularly the Chinese herbal medicine microbial ecological agent of a kind of prevention and treatment dirt chronic obstructive pulmonary disease.
Two, background technology
Chronic obstructive pulmonary disease is the limited disease of a kind of respiratory tract air flow, and this flow limitation carries out sexual development, and is not exclusively reversible.To act on lung tissue relevant with sucking poisonous granule for this disease, thereby produce chronic inflammatory disease.This wherein topmost reason smoking, poisonous smoke are arranged and suck the poisonous granules such as dust relevant.Wherein performance is the most violent, the most significantly case is exactly owing to suck the pneumoconiosis case that dust causes.In industrial process, people can suffer from pneumoconiosis because of the various dust of long-term suction.Pneumoconiosis can cause patient's pulmonary lesion, causes easily simultaneously pulmonary tuberculosis.In case Finding case has above-mentioned disease, the lighter reduces work capacity, and severe one causes that the organ failure of the whole province is dead.Therefore, this class disease is subject to people's attention day by day.At present, the most effective Therapeutic Method of pneumoconiosis is to fill with the lung treatment; But, even fill with the lung treatment, because the injury that the patient is caused is large, expense is high, weak curative effect and be difficult to promote; Simultaneously, for the pneumoconiosis patients of suffering from other symptoms such as pulmonary tuberculosis, can not adopt and fill with the lung treatment; Except filling with the lung treatment, other Drug therapy bronchodilator and antiinflammatory play mitigation to the state of an illness, can not fundamentally remove the pathogenic dust of pulmonary.Therefore, in the urgent need to effectively dust discharge and the new product of effectively treating pneumoconiosis of invention.For the chronic obstructive pulmonary disease that medicated cigarette flue dust or atmospheric aerosol cause, situation also is the same basically, lacks at present effective Therapeutic Method the poisonous granules such as medicated cigarette flue dust or atmospheric aerosol are directly discharged, thereby lack effective treatment means.
Three, summary of the invention
The objective of the invention is the problem that is difficult to treat for above-mentioned chronic obstructive pulmonary disease, provide a kind of evident in efficacy, without any toxicity, side effect, and the cheap Chinese herbal medicine microbial ecological agent that is used for prevention and treatment chronic obstructive pulmonary disease of expense.
The present invention adopts following technical scheme:
The mother solution of preparation microbial ecological agent:
(1) the used probiotic bacteria strain of the present invention is red tea fungus, comprises floras such as mainly comprising acetic acid bacteria, yeast, wherein comprises the acetic acid bacteria that produces Bacterial cellulose; Also can adopt the acetic acid bacteria strain of product Bacterial cellulose of separation and Culture as intending acetobacter xylinum (Acetobacter xylinoides), Bacterium gluconicum (Bacterium gluconicum), the wooden subspecies of acetobacter aceti, also be acetobacter xylinum (Acetobacter xylinum), separate the barms such as the saccharomyces cerevisiae (Saccharomyces cerevisiae) that obtain, rood class yeast (Saccharomycodes ludwigii), schizosaccharomyces pombe (Schizosaecharomyces pombe), candida tropicalis (Candida tropicans), candida krusei (Candidacrusei), the inferior Dbaly yeast of the Chinese (Debaryomyces hansenii), brettanomyce (Brettanyomyces), Kloeckera japonica (Kloeckera), Bayer zygosaccharomyces (Zygosaccharomyces bailiff), saccharomyces pastorianus (Saccharomyces pastorianus Hansen) etc. choose wherein acetic acid bacteria and yeast complex configuration form;
(2) preparation of extracts of Chinese herbal medicine: Chinese medicine weight ratio prescription is: Folium Camelliae sinensis 1-5%, and Radix Glycyrrhizae 1-5%, Flos Chrysanthemi 1-5%, Fructus Momordicae 1-5%, Semen Sterculiae Lychnophorae 1-5%, aqueous solution 75-95% adds Chinese herbal medicine in the aqueous solution and to boil, and filters to obtain extracts of Chinese herbal medicine;
(3) extracts of Chinese herbal medicine that filters is cooled to 30 ℃, the composite flora that the acetic acid bacteria of the red tea fungus mother solution of inoculation 5%-50% or product Bacterial cellulose and yeast form, after 7 days, namely can be used for prevention and the treatment of pneumoconiosis in static cultivation under 25-35 ℃ the environment;
(4) because fluid product is not easy to preserve, with the Chinese herbal medicine microbial ecological agent frozen drying of above-mentioned fermentation finished thoroughly1, form the mycopowder of Chinese herbal medicine microbial ecological agent, but long preservation after the vacuum packaging; During use, the brown sugar water with 1% or honey aqueous solution dissolving mycopowder leave standstill and activated 1-2 as a child, namely can be used for the treatment of pneumoconiosis;
When (5) treating, on the one hand in oral, be used for removing gastral noxious substance, improve the abilities of digestive and absorption of human body, improve human body to the resistance of disease; In addition on the one hand, utilize the small plastic aerosol apparatus to aim at the human body nasal cavity and spray, human body sucks pulmonary by initiatively breathing the bacterium liquid that will atomize; At this moment, the medicinal herb components in the Chinese herbal medicine microbial ecological agent and microbial fermentation solution composition play certain treatment and the effect of releiving to pneumonia; The organic acid that acetic acid bacteria in the Chinese herbal medicine microbial ecological agent, yeast, lactobacillus produce dissolves the poisonous granules such as a part of pulmonary dust, and the body fluid circulation by pulmonary's body excretes; Simultaneously, product cellulose acetate bacterium in the Chinese herbal medicine microbial ecological agent forms the poisonous granules such as Bacterial cellulose parcel pulmonary dust, effect by lung cilium etc. forms the materials such as expectorant, excretes by respiratory tract, thereby gradually reduces the poisonous granules such as dust of pulmonary; Thereby gradually reduce dust to murder by poisoning and the fibrosis of lung tissue, thereby fundamentally eliminate gradually the infective pathogen of chronic obstructive pulmonary disease, reach the target of effective treatment or healing.
The beneficial effect of Chinese herbal medicine microbial ecological agent of the present invention:
(1) treats simultaneously chronic obstructive pulmonary disease from digestive tract and respiratory tract; The treatment of chronic obstructive pulmonary disease is general otherwise pass through oral drug therapy; Otherwise by filling with the lung treatment, therefore, single Therapeutic Method causes effect limited;
(2) eliminate the poisonous granules such as pulmonary's dust at digestive tract by degrading and adsorbing two kinds of mechanism; Microorganism in the microbial ecological agent of the present invention is red tea fungus, it comprises the strains such as acetic acid bacteria, yeast, lactobacillus, and acetic acid bacteria wherein can be divided into again the acetic acid bacteria that produces acetic acid and utilize acetic acid to form the strain of the product Bacterial cellulose such as acetobacter xylinum of Bacterial cellulose.Has the characteristic of absorption, enwrapped granule owing to produce the Bacterial cellulose of the probiotic bacterias such as the acetobacter xylinum generation of Bacterial cellulose, therefore, it sprays into after the human lung, it can work in coordination with other acetic acid bacterias, yeast produces the poisonous granules such as Bacterial cellulose parcel pulmonary dust, thereby plays the special therapeutical effect to chronic obstructive pulmonary disease; Simultaneously, the organic acid that the probiotic bacterias such as acetic acid bacteria, yeast produce can effectively reduce the poisonous granules such as part dust particle in the lung, thereby plays poisonous granules such as eliminating dust by Degradation; Thereby gradually reduce the poisonous granules such as dust to murder by poisoning and the fibrosis of lung tissue;
(3) not only have no side effect, and the toxic and side effects of the other drug of can sterilizing, can life-time service; Red tea fungus or other used probiotic combinations floras are through long-term medical science and production practices and studies have shown that human body and the nontoxic probiotic bacteria flora of animal, so all be safe to environment human and on every side, it has very strong inhibitory action to the growth of other antibacterials or fungus, simultaneously, it also has very strong facilitating digestion effect.
(4) easy to use; The Chinese herbal medicine microbial ecological agent both can be oral, and the treatment of can spraying again is very convenient; Because this finished product has no side effect, therefore both can be used for prevention, can be used for the treatment of again.
Four, the specific embodiment
Further specify the present invention program and effect below in conjunction with embodiment.
Embodiment 1
(1) mother solution of preparation microbial ecological agent: the used probiotic bacteria strain of the present invention is red tea fungus, and the red tea fungus mother solution is the red tea fungus bacterium liquid of cultivating 15 days, and culture medium prescription is Folium Camelliae sinensis 5%, sucrose 5%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.1%.With the 1L distilled water put into pot boiled after, add successively sucrose, Folium Camelliae sinensis, ammonium sulfate, potassium dihydrogen phosphate, after again boiled in water, with gauze elimination Folium Camelliae sinensis, naturally cooling to 30 ℃ is distributed in 1 liter the wide-mouth vial, access red tea fungus strain mother solution, kraft paper seal rear 30 ℃ and leave standstill and cultivate 7--15 days as the red tea fungus mother solution;
(2) preparation of extracts of Chinese herbal medicine: Chinese medicine weight ratio prescription is: Folium Camelliae sinensis 1%, and Radix Glycyrrhizae 1%, Flos Chrysanthemi 1%, Fructus Momordicae 1%, Semen Sterculiae Lychnophorae 1%, aqueous solution 95% adds Chinese herbal medicine in the aqueous solution and to boil, and filters to obtain extracts of Chinese herbal medicine;
(3) extracts of Chinese herbal medicine that filters is cooled to 30 ℃, the red tea fungus mother solution of inoculation 20% after 7 days, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease in static cultivation under 30 ℃ the environment;
(4) because fluid product is not easy preservation, the Chinese herbal medicine microbial ecological agent of above-mentioned fermentation finished thoroughly1 is added protective agent sodium glutamate 5g/L, trehalose 20g/L, lactose 5g/L, skimmed milk 20g/L, form the mycopowder of Chinese herbal medicine microbial ecological agent after the spray drying, vacuum-packed rear the preservation 6 months-12 months; During use, the brown sugar water with 1% or honey aqueous solution dissolving mycopowder leave standstill and activated 1-2 as a child, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease;
When (5) treating, on the one hand in oral, be used for removing gastral noxious substance, improve the abilities of digestive and absorption of human body, improve human body to the resistance of disease; In addition on the one hand, utilize the small plastic aerosol apparatus to aim at the human body nasal cavity and spray, human body sucks pulmonary by initiatively breathing the bacterium liquid that will atomize; At this moment, the medicinal herb components in the Chinese herbal medicine microbial ecological agent and microbial fermentation solution composition play certain treatment and the effect of releiving to pneumonia; The organic acid that acetic acid bacteria in the Chinese herbal medicine microbial ecological agent, yeast, lactobacillus produce dissolves a part of pulmonary dust, and the body fluid circulation by pulmonary's body excretes; Simultaneously, product cellulose acetate bacterium in the Chinese herbal medicine microbial ecological agent forms Bacterial cellulose parcel pulmonary dust, and the effect by lung cilium etc. forms the materials such as expectorant, excretes by respiratory tract, thereby gradually reduces the dust of pulmonary.This is at present except filling with the lung cleaning technique, unique can be from treatment product and the technology of the poisonous granules such as pulmonary's dust discharge dust.
Embodiment 2
(1) mother solution of preparation microbial ecological agent: the used probiotic bacteria strain of the present invention is the composite bacteria that gluconic acid acetobacter, saccharomyces cerevisiae complex configuration form; Gluconic acid acetobacter (Gluconacetobacter hansenii GGMCC1671) bacterial strain and saccharomyces cerevisiae (Saccharomyces cerevisiae CCMCC1670) are bought from China Microbial Culture Preservation Commission common micro-organisms center; Culture medium is that culture medium prescription is Folium Camelliae sinensis 5%, sucrose 5%, ammonium sulfate 0.1%, potassium dihydrogen phosphate 0.1%;
(2) preparation of extracts of Chinese herbal medicine: Chinese medicine weight ratio prescription is: Folium Camelliae sinensis 1%, and Radix Glycyrrhizae 1%, Flos Chrysanthemi 1%, Fructus Momordicae 1%, Semen Sterculiae Lychnophorae 1%, aqueous solution 95% adds Chinese herbal medicine in the aqueous solution and to boil, and filters to obtain extracts of Chinese herbal medicine; The extracts of Chinese herbal medicine that filters is cooled to 30 ℃, and the composite bacteria mother solution of inoculation 20% after 7 days, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease in static cultivation under 30 ℃ the environment;
(3) because fluid product is not easy preservation, the Chinese herbal medicine microbial ecological agent of above-mentioned fermentation finished thoroughly1 is added protective agent sodium glutamate 5g/L, trehalose 20g/L, lactose 5g/L, skimmed milk 20g/L, form the mycopowder of Chinese herbal medicine microbial ecological agent after the spray drying, vacuum-packed rear the preservation 6 months-12 months; During use, the brown sugar water with 1% or honey aqueous solution dissolving mycopowder leave standstill and activated 1-2 as a child, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease;
Chinese herbal medicine microbial ecological agent of the present invention has carried out clinical use and observation of curative effect to most typical Pneumoconiosis Cases in the chronic obstructive pulmonary disease, and the result is as follows: suffer from 1,2,3 phase Pneumoconiosis Patients totally 38 examples, age 30-50 year, wherein suffer from pneumoconiosis I phases 10 example, II phases 25 example, III phases 3 example.The unified treatment of all patients, every day, oral Chinese herbal medicine microbial ecological agent was 1-2 time, each 20-50ML, spraying treatment 1-2 time, each 3-5 minute, continuous 6 months.Carry out clinical observation and show through the patient, the symptoms such as patient's cough, uncomfortable in chest, dyspnea have obvious alleviation, and simultaneously, patient's food-intake obviously increases, and body constitution is obviously improved; Detection shows through rabat: focus obviously absorbs 15 examples of improvement, and 18 examples that take a favorable turn without significant change 5 examples, mainly are the smaller patients of I phase symptom.Therefore, in general, curative effect is very significant.Simultaneously; in the process for the treatment of; the material that contains dust or mine dust in the sputum that most of patient spues; obvious dust discharge reaction appears; especially a large amount of dust discharge reactions sometimes appears in III phase and II phase Pneumoconiosis Patients in the therapeutic process, and I phase or the slight patient of symptom; as seen the dust-exhausting quantity that occurs still seldom has a large amount of dust discharge reactions.Have special dust discharge effect behind this Chinese herbal medicine microbial ecological agent treatment of this sufficient proof patient.The below describes two gold mine workmans' detailed treatment situation in detail: Chinese herbal medicine microbial ecological agent of the present invention, two Pneumoconiosis Patients in the treatment case treatment and those benefits in detail observation have been carried out, the result is as follows: Chinese herbal medicine microbial ecological agent treatment patient A observation of curative effect: patient A is pneumoconiosis I phase patient, man 46 years old, from 1987 at somewhere gold mine or coal mine working, found pneumoconiosis in 2006.In JIUYUE, 2010 begins oral Chinese herbal medicine microbial ecological agent 1 time every day, each 20-50-100ML, spraying treatment 1-2 time, 3-5 minute at every turn, continuous 6 months.Carry out clinical observation and show through the patient, the symptoms such as patient's cough, uncomfortable in chest, dyspnea have obvious alleviation, and simultaneously, patient's food-intake obviously increases, and body constitution is obviously improved; Detection shows through rabat: focus obviously absorbs improvement; In therapeutic process, the patient is reported in the mine dust granule that comprises black in the sputum of telling, and after this confirmation utilizes the treatment of this Chinese herbal medicine microbial ecological agent, can discharge mine dust from patient's pulmonary, has also confirmed this point from the result of variations of rabat.
Chinese herbal medicine microbial ecological agent treatment patient B observation of curative effect: patient B is pneumoconiosis II phase patient, the man, and 45 years old, work from 1987 at the somewhere gold mine, be diagnosed as II phase pneumoconiosis in 2004, cough is serious, serious rest to affecting every day, essentially no work capacity.Patient B oral Chinese herbal medicine microbial ecological agent every day in 2011 1 time, each 50-100ML, spraying treatment 1-2 time, each 3-5 minute, continuous 6 months.Carry out clinical observation and show through the patient, the symptoms such as patient's cough, uncomfortable in chest, dyspnea have obvious alleviation, and simultaneously, patient's food-intake obviously increases, and body constitution is obviously improved; Detection shows through rabat: focus obviously absorbs improvement; In therapeutic process, the patient is reported in the mine dust granule that comprises black in the sputum of telling, but general amount is fewer, occurs the phenomenon of dust discharge after one week of medication; But, after 4 weeks of medication, in a sputum, see the mine dust of a large amount of black, after this abundant this confirmation utilizes this Chinese herbal medicine microbial ecological agent treatment, can discharge mine dust from patient's pulmonary.Treating in detail case for these two shows: utilize this Chinese herbal medicine microbial ecological agent can effectively treat poisonous granules such as discharging mine dust and treatment pneumoconiosis and other chronic obstructive pulmonary disease.
Claims (6)
1. a Chinese herbal medicine microbial ecological agent that prevents and treat chronic obstructive pulmonary disease is characterized in that the used probiotic bacteria strain of the present invention is red tea fungus, mainly comprises the floras such as acetic acid bacteria, yeast, wherein comprises the acetic acid bacteria that produces Bacterial cellulose; Also can adopt the acetic acid bacteria strain of product Bacterial cellulose of separation and Culture as intending acetobacter xylinum (Acetobacter xylinoides), Bacterium gluconicum (Bacterium gluconicum), the wooden subspecies of acetobacter aceti, also be acetobacter xylinum (Acetobacter xylinum), separate the barms such as the saccharomyces cerevisiae (Saccharomyces cerevisiae) that obtain, rood class yeast (Saccharomycodes ludwigii), schizosaccharomyces pombe (Schizosaecharomyces pombe), candida tropicalis (Candida tropicans), candida krusei (Candidacrusei), the inferior Dbaly yeast of the Chinese (Debaryomyces hansenii), brettanomyce (Brettanyomyces), Kloeckera japonica (Kloeckera), Bayer zygosaccharomyces (Zygosaccharomyces bailiff), saccharomyces pastorianus (Saccharomyces pastorianus Hansen) etc. choose wherein product Bacterial cellulose acetic acid bacteria and yeast complex configuration form.
2. the Chinese herbal medicine microbial ecological agent of a kind of prevention as claimed in claim 1 and treatment chronic obstructive pulmonary disease is characterized in that its used probiotic bacteria strain comprises acetic acid bacteria and the yeast that can produce Bacterial cellulose.
3. the Chinese herbal medicine microbial ecological agent of a kind of prevention as claimed in claim 1 and treatment chronic obstructive pulmonary disease is characterized in that its Chinese herbal medicine formula is: Folium Camelliae sinensis 1-5%, Radix Glycyrrhizae 1-5%, Flos Chrysanthemi 1-5%, Fructus Momordicae 1-5%, Semen Sterculiae Lychnophorae 1-5%, aqueous solution 75-95%.
4. the Chinese herbal medicine microbial ecological agent of a kind of prevention as claimed in claim 1 and treatment chronic obstructive pulmonary disease, its feature is cooled to 30 ℃ at the extracts of Chinese herbal medicine that will filter, the probiotic bacteria strain mother solution of inoculation 5%-50%, after 7 days, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease in static cultivation under 25-35 ℃ the environment.
5. the Chinese herbal medicine microbial ecological agent of a kind of prevention as claimed in claim 1 and treatment chronic obstructive pulmonary disease, its feature is at the Chinese herbal medicine microbial ecological agent frozen drying with above-mentioned fermentation finished thoroughly1, form the mycopowder of Chinese herbal medicine microbial ecological agent, but long preservation after the vacuum packaging; During use, the brown sugar water with 1% or honey aqueous solution dissolving mycopowder leave standstill and activated 1-2 as a child, namely can be used for prevention and the treatment of chronic obstructive pulmonary disease.
6. the Chinese herbal medicine microbial ecological agent of a kind of prevention as claimed in claim 1 and treatment chronic obstructive pulmonary disease, its feature prevent with mode oral and that spray simultaneously and treat when prevention and treatment.
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| CN105287733A (en) * | 2015-10-29 | 2016-02-03 | 海南木辣达生物科技有限公司 | Moringa oleifera ferment for preventing and treating pneumoconiosis and preparation method thereof |
| CN106244410A (en) * | 2016-08-31 | 2016-12-21 | 金寨县劲春银杏树种植专业合作社 | A kind of gingkgo vinegar beverage and preparation method thereof |
| CN107582616A (en) * | 2017-09-06 | 2018-01-16 | 海南青牧原实业有限公司 | A kind of piglet Chinese herbal medicine black tea bacteria preparation and preparation method thereof |
| CN108543008A (en) * | 2018-04-25 | 2018-09-18 | 惠州嘉联生物科技开发有限公司 | A kind of composite enzyme of preventing respiratory tract infection and preparation method thereof |
| CN114854610A (en) * | 2022-05-23 | 2022-08-05 | 江苏大学 | Preparation method of Debaryomyces hansenii freeze-dried microbial inoculum and application of Debaryomyces hansenii freeze-dried microbial inoculum in controlling soft rot of strawberries after picking |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105287733A (en) * | 2015-10-29 | 2016-02-03 | 海南木辣达生物科技有限公司 | Moringa oleifera ferment for preventing and treating pneumoconiosis and preparation method thereof |
| CN106244410A (en) * | 2016-08-31 | 2016-12-21 | 金寨县劲春银杏树种植专业合作社 | A kind of gingkgo vinegar beverage and preparation method thereof |
| CN107582616A (en) * | 2017-09-06 | 2018-01-16 | 海南青牧原实业有限公司 | A kind of piglet Chinese herbal medicine black tea bacteria preparation and preparation method thereof |
| CN108543008A (en) * | 2018-04-25 | 2018-09-18 | 惠州嘉联生物科技开发有限公司 | A kind of composite enzyme of preventing respiratory tract infection and preparation method thereof |
| CN114854610A (en) * | 2022-05-23 | 2022-08-05 | 江苏大学 | Preparation method of Debaryomyces hansenii freeze-dried microbial inoculum and application of Debaryomyces hansenii freeze-dried microbial inoculum in controlling soft rot of strawberries after picking |
| CN114854610B (en) * | 2022-05-23 | 2024-07-12 | 江苏大学 | Preparation method of Debaryomyces hansenii freeze-dried microbial inoculum and application of freeze-dried microbial inoculum in control of soft rot of strawberries after harvest |
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Application publication date: 20131030 |