CN103371144A - Sex attractant of cotton bollworm and lure as well as preparation method and application thereof - Google Patents

Abstract

The invention provides a sex attractant of cotton bollworm. The sex attractant comprises the following components in percentage by weight: 90-99% of (Z)-11-hexadecenal, 0.9-9.9% of (Z)-9-hexadecenal and 0.1-0.5% of (Z)-9-tetradecenal. The invention also provides a lure containing the sex attractant of the cotton bollworm and a preparation method thereof as well as application of the sex attractant of the cotton bollworm and the lure. The sex attractant of the cotton bollworm, provided by the invention, is capable of obviously increasing the trapping amount and the accuracy and high in cost-performance ratio and has wide-range popularization and application values. The three matters needed in lure preparation can be simultaneously diluted and dropwise added on a carrier with no need for other operating steps and the use method is simple. By using the sex attractant of the cotton bollworm prepared by adopting the method provided by the invention, the trapping effect can be remarkably increased.

Description

Sexual attractant and lure core for cotton bollworm and its preparation method and application

technical field

The invention relates to a sex attractant for cotton bollworm, a lure core containing the sex attractant, a preparation method thereof, and an application of the sex attractant and the lure core.

Background technique

Cotton bollworm is a major worldwide pest with a wide range of hosts and strong adaptability and reproductive ability. It often breaks out into disasters and causes serious damage to major field crops and vegetables such as corn, soybean, and cotton. The control of cotton bollworm using informational substances has the characteristics of high efficiency, non-toxicity, no harm to other organisms, and no pollution to the entire ecological environment, which is in line with the road of global green ecological agriculture. Cotton bollworm lures used in the past are mainly composed of (Z)-11-hexadecenal (Z-11-hexadecenal) and (Z)-9-hexadecenal (Z-9-hexadecenal) two chemical substances However, with the generation of anti-interference ability of cotton bollworm to artificial sex attractants, male moths showed more precise selective adaptation to accept and respond to the chemical signals of sex semiochemicals released by female moths.

In order to obtain an efficient and accurate sexual attractant for cotton bollworm, predict the cotton bollworm and interfere with mating to achieve the purpose of preventing and controlling the insect, it is urgent to develop and utilize a new type of high-efficiency sexual attractant for cotton bollworm.

Contents of the invention

Therefore, an object of the present invention is to provide a highly efficient and precise sex attractant for cotton bollworm, so as to improve its trapping precision and quantity.

Another object of the present invention is to provide a lure core containing the sexual attractant for cotton bollworm of the present invention.

Another object of the present invention is to provide a method for preparing a sex attractant lure for cotton bollworm.

Another object of the present invention is to provide the above-mentioned sexual attractant for cotton bollworm and the application of the lure core.

The study found that in addition to (Z)-11-hexadecenal and (Z)-9-hexadecenal in the gonads of the cotton bollworm, there is also (Z)-9-tetradecenal (Z-9-tetradecenal), (Z)-7-hexadecenal (Z-7-hexadecenal) and hexadecanal (hexadecanal) and many other substances, therefore, these substances have increased attractant effect possibility. The present invention has finally determined that the technical scheme of the present invention is as follows through further research:

In one aspect, the present invention provides a sex attractant for cotton bollworm, comprising by weight of the sex attractant: 90-99% of (Z)-11-hexadecenal, 0.9-9.9% of (Z)-9 - Hexadecenal, and 0.1-0.5% (Z)-9-tetradecenal; the sum of the weight percentages of each component in the sex attractant is 100%.

Preferably, the sex attractant comprises, by weight of the sex attractant: 98.3% of (Z)-11-hexadecenal, 1.4% of (Z)-9-hexadecenal, and 0.3% (Z)-9-Tetradecenal.

Preferably, the purity of (Z)-11-hexadecenal, (Z)-9-hexadecenal and (Z)-9-tetradecenal in the sex attractant is higher than 95% %.

In a preferred embodiment of the present invention, the sex attractant is composed of (Z)-11-hexadecenal, (Z)-9-hexadecenal and (Z)-9-tetradecene Aldehydes are composed of three components.

On the other hand, the present invention provides a sex attractant core for cotton bollworm, which includes a carrier and the above-mentioned sex attractant for cotton bollworm adsorbed on the carrier.

The carrier can be natural rubber or composite rubber stoppers of various colors such as red, green or gray. The shape of the rubber stopper can be anti-mouth type or other shapes. Also within the scope of protection of this patent application.

Preferably, in the lure, the carrier is a rubber carrier, preferably a rubber stopper, such as a reverse mouth rubber stopper.

Preferably, in the lures, the single lure contains 0.1-1 mg, preferably 0.5-1 mg, of the cotton bollworm sex attractant.

In another aspect, the present invention provides a method for preparing a sex attractant lure for cotton bollworm, comprising the following steps:

(1) Dilute the above-mentioned sex attractant for cotton bollworm with solvent;

(2) Add the diluted solution obtained in the previous step onto the carrier dropwise, and obtain the lure after the solvent is completely evaporated.

Preferably, in the step (1), the sex attractant is diluted to 1-10 μg/μl with n-hexane.

The solvent used for dilution can also be organic solvents such as n-hexane, ether or petroleum ether.

Preferably, the carrier in the step (2) is a rubber carrier, preferably a rubber stopper, such as a reverse-mouthed rubber stopper.

Preferably, the single lure core contains 0.1-1 mg, preferably 0.5-1 mg, of the sex attractant for cotton bollworm.

Preferably, the lure can be used after the solvent is completely volatilized, or stored in a -20°C refrigerator for future use.

In yet another aspect, the present invention provides the use of the above-mentioned sex attractant for cotton bollworm or the lure core of the above-mentioned sex attractant for cotton bollworm in predicting, interfering with mating, trapping cotton bollworm, and/or controlling cotton bollworm.

In agricultural and forestry production, the sex attractant is used to trap cotton bollworms, predict the cotton bollworms and interfere with mating to achieve the purpose of preventing and controlling the bollworms.

The advantages of the present invention are:

1. The sex attractant for cotton bollworm of the present invention can significantly improve the trapping capacity and accuracy, and is cost-effective, and has a wide range of popularization and application value.

2. The method of use is simple. The three substances required to prepare the lure can be diluted at the same time and added dropwise on the carrier without any other operation steps. The cotton bollworm lure formulated according to the method of the invention can significantly increase the moth lure effect.

Detailed ways

The present invention can be further illustrated by the following examples, but the present invention is not limited to the following examples.

Example 1

From July 24 to September 9, 2011, a new type of attractant for cotton bollworm was tested in cotton fields in Raoyang County, Hebei Province. Test substances: (Z)-9-tetradecenal (Z9-14: Al), (Z)-7-hexadecenal (Z7-16: Al) and hexadecenal ( 16:Al) and B: (Z)-11-hexadecenal (Z11-16:Al)+(Z)-9-hexadecenal (Z9-16:Al) used together, each chemical substance The purity is higher than 95%. Each mixture was prepared according to the following weight percentages (gonadal extraction analysis):

B＝Z11-16: Al 98.6%+Z9-16: Al 1.4%;

E＝Z11-16:Al 95.1%+Z9-16:Al 1.3%+16:Al 3.6%;

F＝Z11-16: Al 97.6%+Z9-16: Al 1.4%+Z7-16: Al 1%;

J = Z11-16: Al 98.3% + Z9-16: Al 1.4% + Z9-14: Al 0.3%.

Dilute the above four mixtures (B, E, F and J) with n-hexane to three concentration gradients of 1 μg/μl, 5 μg/μl and 10 μg/μl, and add 100 μl dropwise to each reverse-port rubber stopper carrier to dilute well solution, the solute contents were 0.1, 0.5 and 1mg. Place it at room temperature for about 2 hours until the solvent is completely volatilized to make a sex lure core. 4 mixtures, 3 concentration gradients for each mixture, a total of 12 treatments, 15 individual lures were prepared for each treatment, and field experiments were carried out. When conducting trapping experiments in the field, a prepared sex lure was randomly selected in each treatment and fixed on the upper end of the pot-type trap with a thin wire, and the trap was fixed at a position consistent with the height of the crops in the field with a bamboo pole. Arrange in the crop field, and record the position of each treatment, each trap is more than 30 meters apart. The lures were replaced every week, and the positions of the traps were changed to avoid the influence of different habitats on the lures. The test results are shown in Table 1. The statistical analysis of the data was carried out using one-way ANOVA, LSD multiple-comparison (P=0.05) method, in SPSS. 17.0 (SPSS Inc., Chicago, IL, USA) software.

Table 1 Attraction of cotton bollworm male moths under different doses of each mixture

As shown in Table 1, under the doses of 0.1, 0.5 and 1 mg, the mixture J showed a better attractant effect than B, E and F, especially at the doses of 0.5 and 1 mg, compared with the other three mixtures, the mixture J showed The difference is significant, the average moth-attracting amount of the J mixture is more than three times that of the other mixtures, which significantly increases the moth-attracting effect. Therefore the mixture J=Z11-16:Al+Z9-16:Al+Z9-14:Al has a good moth-attracting effect in the dose range of 0.1 to 1 mg.

Example 2

Prepare (Z)-11-hexadecenal, (Z)-9-hexadecenal and (Z)-9-tetradecenal according to the following 3 weight percentages:

J ₁ = Z11-16: Al 90% + Z9-16: Al 9.5% + Z9-14: Al 0.5%,

J ₂ = Z11-16: Al 95% + Z9-16: Al 4.7% + Z9-14: Al 0.3%,

J ₃ =Z11-16: Al 99%+Z9-16:Al 0.9%+Z9-14:Al 0.1%,

They were diluted with n-hexane to 5 μg/μl respectively, and 100 μl of the diluted solution was added dropwise to each reverse-port rubber stopper carrier, and the solute content was 0.5 mg respectively. Place it at room temperature for about 2 hours until the solvent is completely volatilized, then make a sex lure core and conduct a field test. When conducting trapping experiments in the field, a prepared sex lure was randomly selected in each treatment and fixed on the upper end of the pot-type trap with a thin wire, and the trap was fixed at a position consistent with the height of the crops in the field with a bamboo pole. Arranged in the crop field, each trap is more than 30 meters apart, and the male moths are recorded and removed every day. The lures were replaced every week, and the positions of the traps were changed to avoid the influence of different habitats on the lures. The test results are shown in Table 2. The statistical analysis of the data adopts the one-way ANOVA, LSD multiple-comparison (P=0.05) method, in SPSS .17.0 (SPSS Inc., Chicago, IL, USA) software.

Table 2 contains the moth-attracting effect of the mixture of components in different proportions

date J ₁ J ₂ J ₃ 7-24 6 0 2 7-25 5 1 3 7-26 0 0 0 7-27 0 0 6 7-28 1 0 1 7-29 0 0 1 7-30 1 0 0 7-31 8 1 5 8-1 3 1 3 8-2 5 0 2

8-3 1 0 0 8-4 6 0 2 8-5 3 0 1 8-6 6 0 0 8-7 0 0 0 8-8 0 0 5 8-9 2 6 4 8-10 0 8 5 8-11 0 0 0 8-12 0 8 3 8-13 0 2 0 8-14 0 2 3 8-15 0 10 5 8-16 0 5 6 8-17 0 7 0 8-18 0 2 0 8-19 0 3 3 8-20 1 1 1 8-21 1 0 0 8-22 0 5 0 8-23 2 0 5 8-24 0 4 0 8-25 0 0 0 8-26 1 0 0 8-27 0 0 0 8-28 0 0 3 8-29 0 0 0 8-30 0 0 1 8-31 0 0 0 9-1 0 2 0 9-2 0 0 0

9-3 0 0 0 9-4 0 0 0 9-5 0 0 0 9-6 0 0 0 9-7 0 0 0 9-8 0 0 0 9-9 0 0 0 sum 52 68 70 average 1.08 1.42 1.46

The above data show that the sex attractant for cotton bollworm of the present invention has a good effect of attracting moths under these three ratios.

Claims (11)

1. cotton bollworm sex attractant comprises the weighing scale by sex attractant:

(Z)-11-hexadecenal of 90～99%,

0.9 (Z)-9-hexadecenal of～9.9%, and

0.1 (Z)-9-fulure of～0.5%;

The percetage by weight sum of each component is 100% in the described sex attractant.

2. sex attractant according to claim 1 comprises the weighing scale by sex attractant:

(Z)-11-hexadecenal of 98.3%,

(Z)-9-hexadecenal of 1.4%, and

(Z)-9-fulure of 0.3%.

3. sex attractant according to claim 1 and 2, wherein the purity of (Z)-11-hexadecenal, (Z)-9-hexadecenal and (Z)-9-fulure all is higher than 95%.

4. a cotton bollworm sex attractant lures core, describedly lures core to comprise carrier and is adsorbed on each described cotton bollworm sex attractant in the claims 1 to 3 on the described carrier.

5. the core that lures according to claim 4, wherein, described carrier is rubber carrier, the preferred rubber plug is such as anti-mouthful of formula rubber stopper.

6. according to claim 4 or the 5 described cores that lure, wherein, described single luring contained cotton bollworm sex attractant 0.1～1mg, preferred 0.5～1mg on the core.

7. a cotton bollworm sex attractant lures the preparation method of core, may further comprise the steps:

(1) with each described cotton bollworm sex attractant solvent dilution in the claims 1 to 3;

(2) solution after the dilution of gained of upper step is added drop-wise on the carrier, after solvent volatilizees fully, namely gets and lure core.

8. preparation method according to claim 7 is diluted to 1～10 μ g/ μ l with n-hexane with sex attractant in the wherein said step (1).

9. preparation method according to claim 7, the carrier in the wherein said step (2) is rubber carrier, the preferred rubber plug is such as anti-mouthful of formula rubber stopper.

10. each described preparation method in 9 according to claim 7, wherein said single luring contained cotton bollworm sex attractant 0.1～1mg, preferred 0.5～1mg on the core.

11. in the claims 1 to 3 in each described cotton bollworm sex attractant or the claim 4 to 6 each described cotton bollworm sex attractant lure core cotton bollworm carried out prediction, disturbing mating, the trapping cotton bollworm, and/or the application in the control cotton bollworm.