CN103329736A - Breeding method for increasing production of bacterial-wilt-resisting mulberry tree - Google Patents
Breeding method for increasing production of bacterial-wilt-resisting mulberry tree Download PDFInfo
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- CN103329736A CN103329736A CN2013102554924A CN201310255492A CN103329736A CN 103329736 A CN103329736 A CN 103329736A CN 2013102554924 A CN2013102554924 A CN 2013102554924A CN 201310255492 A CN201310255492 A CN 201310255492A CN 103329736 A CN103329736 A CN 103329736A
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Abstract
The invention discloses a breeding method for increasing production of a bacterial-wilt-resisting mulberry tree. The breeding method is characterized in that a bacterial-wilt-resisting variety of No.10 bacterial-wilt-resisting variety is selected as a tree stock, the variety of lund 40 which has high production and is excellent is selected as scion, and breeding is completed through grafting. According to the breeding method disclosed by the invention, the No.10 bacterial-wilt-resisting variety is changed into a good variety of a lund 40 good mulberry, and the bacterial wilt resistance of the No.10 bacterial-wilt-resisting variety is reserved. The breeding method disclosed by the invention is simple in structure; and compared with a traditional disease-resisting breeding method, the breeding method has the characteristics of short time and quick effect.
Description
Technical field
The present invention relates to the kind method for planting of a kind of resistance to bacterial wilt mulberry tree, be specifically related to a kind of breeding method that improves resistance to bacterial wilt mulberry tree output.
Background technology
Mulberry tree bacterial wilt (Pseudomonas solanacerum Smith) belongs to phytosis, has another name called " fusarium wilt ", popular name " pest mulberry ", and cause of disease belongs to pseudomonas.This disease is that a kind of soil passes property bacteriosis, have morbidity anxious, spread rapidly, harm is serious, prevent and treat characteristics such as difficulty, is one of main mulberry tree disease that production has crushing harm to silkworm and mulberry.The diseased plant stubble body of sending out sick soil and being lost in the soil is the main source of infection of this disease, farming activities by rainfall, flowing water and people and animals causes and infects for the first time, germ can pass through wound, insect population etc. and invade root tissue, also can invade plant by injured stem.
At present, tackling the most effective means in bacterial wilt morbidity silkworm district is exactly the mulberry races of plantation resistance to bacterial wilt, anti-blue or green No. 10 is to generally acknowledge best anti-mulberry bacterial wilt kind at present, but relatively there is the shortcoming that food value of leaf poor performance blade is thinner and mulberry leaf output is on the low side with good mulberry races anti-blue or green No. 10, and adopt traditional seed selection kind method, time is long, and it is slow to take effect.Plant the predicament that the anti-blue or green No. 10 mulberry tree food value of leaf are relatively poor and output is on the low side for solving in the region of disease, necessary seed selection is disease-resistant, the kind of high yield, high-quality, but research effect is bad, does not also select desirable like this mulberry races at present.
Summary of the invention
For overcoming the defective of prior art, of the present inventionly be to provide a kind of breeding method that improves resistance to bacterial wilt mulberry tree output, make the mulberry tree in morbidity silkworm district reach both resistance to bacterial wilt, again high yield, high-quality.
The technical solution adopted in the present invention is as follows for achieving the above object:
A kind of breeding method that improves resistance to bacterial wilt mulberry tree output, it carries out according to following steps in turn:
1) selects stock: choose the resistance to bacterial wilt kind and make stock anti-blue or green No. 10, prune stock;
2) select scion: choose human relations 40 good mulberries and do scion;
3) grafting: above-mentioned stock and scion are connect the grafting of method row according to bag, the intact film band of binding of grafting;
4) management: in time untie the film band during rudiment of fringe bar, bud is exposed, remove the anvil bud, to human relations 40 good mulberry normal growths.
The morning of germinateing is selected in scion for use among the present invention, blade is big and thick, the food value of leaf good, output is high, vigorous, the anti-human relations 40 good mulberries of cutting down of cutting that grow, and can effectively improve the output of mulberry tree in enough short time; After the rudiment, remove the growth that the anvil tooth can promote fringe bar bud, ensure the grafting survival rate, thereby guarantee human relations 40 branches energy normal growth, after the growth in 2 years, its branch will improve the resistance of bacterial wilt, and longer its resistance of time is stronger.
The present invention can also realize by following preferred scheme:
As of the present invention preferred embodiment a kind of, prune the plant height 20-40cm of stock in the step 1), and keep the healthy and strong branch of 2-3 bar.Preferable stock plant height 30cm wherein.
As of the present invention preferred embodiment a kind of, step 2) the full human relations of annual hibernaculum spore 40 good mulberry branchs are selected in scion in.
As of the present invention preferred embodiment a kind of, the selection of time of step 3) grafting is spring.
Compared to existing technology, beneficial effect of the present invention is: breeding method of the present invention utilizes grafting method will resist green grass or young crops to change into improved seeds human relations 40 No. 10, and has kept anti-blue or green No. 10 resistance to bacterial wilt.This method is simple to operate, compares with existing traditional seed selection disease resistant and breeding method, has short, instant effect characteristics of time.
Below in conjunction with concrete embodiment the present invention is described in further detail.
Embodiment
Embodiment 1
A kind of breeding method that improves resistance to bacterial wilt mulberry tree output, it carries out according to following steps in turn:
1) selects stock: choose the resistance to bacterial wilt kind and make stock anti-blue or green No. 10, prune stock plant height 20cm, keep the branch of 2 stalwartnesses;
2) select scion: choose the full human relations of annual hibernaculum spore 40 good mulberries and do scion;
3) grafting: above-mentioned stock and scion are connect the grafting of method row according to bag, the intact film band of binding of grafting;
4) management: in time untie the film band during rudiment of fringe bar, bud is exposed, remove the anvil bud, to human relations 40 good mulberry normal growths.
Embodiment 2
A kind of breeding method that improves resistance to bacterial wilt mulberry tree output, it carries out according to following steps in turn:
1) selects stock: choose the resistance to bacterial wilt kind and make stock anti-blue or green No. 10, prune stock plant height 30cm, keep the branch of 3 stalwartnesses;
2) select scion: choose the full human relations of annual hibernaculum spore 40 good mulberries and do scion;
3) grafting: above-mentioned stock and scion are connect the grafting of method row according to bag, the intact film band of binding of grafting;
4) management: in time untie the film band during rudiment of fringe bar, bud is exposed, remove the anvil bud, to human relations 40 good mulberry normal growths.
Embodiment 3
A kind of breeding method that improves resistance to bacterial wilt mulberry tree output, it carries out according to following steps in turn:
1) selects stock: choose the resistance to bacterial wilt kind and make stock anti-blue or green No. 10, prune stock plant height 40cm, keep the branch of 3 stalwartnesses;
2) select scion: choose the full human relations of annual hibernaculum spore 40 good mulberries and do scion;
3) grafting: above-mentioned stock and scion are connect the grafting of method row according to bag, the intact film band of binding of grafting;
4) management: in time untie the film band during rudiment of fringe bar, bud is exposed, remove the anvil bud, to human relations 40 good mulberry normal growths.
Laboratory qualification:
2 years grafting branches of clip are identified grafting branch disease resistance at illumination box with the water planting cultural method then by the inoculation ralstonia solanacearum, compare with human relations 40 good mulberry races branches, and the selection of branch requires the grafting branch unanimity with experiment.Experimental program is 2 processing, 3 repetitions, and every is repeated is 3 bottles, every bottle is 1 branch, 30 ℃ of experiment humidity 100%, temperature, the concentration of ralstonia solanacearum is 10
9Cfu/ml, this condition is suitable ralstonia solanacearum growth.The withered time ratio of grafting branch contrasts slow 2 days, identifies that the grafting branch is than the better resistance of human relations 40 good mulberry branchs.
Field experiment is identified:
Directly water the method evaluation grafting of ralstonia solanacearum to the disease resistance of bacterial wilt at branch inoculation ralstonia solanacearum and at soil in the land for growing field crops, compare inoculation ralstonia solanacearum and require grafting branch unanimity with experiment in the selection that soil waters the ralstonia solanacearum branch with human relations 40 good mulberry races branches.Experimental program is 2 processing, 3 repetitions, and each repeats is 3 strain mulberry treies, the concentration of ralstonia solanacearum is 10
9The amount that cfu/ml, soil water ralstonia solanacearum is every strain 100 ml, and the amount of inoculation ralstonia solanacearum is each branch 20ul.By the observation in 1 year, human relations 40 mulberry races were withered in the dust and grafting is gone back well-grown now, identify that the grafting branch is than the better resistance of human relations 40 branches.
After identify in chamber, land for growing field crops by experiment, illustrate that be feasible with grafting method to the resistance to bacterial wilt that improves grafting, can promote this method in the silkworm district that has bacterial wilt to take place and improve the output of mulberry tree.
Above-mentioned embodiment only is preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, the variation of any unsubstantiality that those skilled in the art does on basis of the present invention and replacement all belong to the present invention's scope required for protection.
Claims (4)
1. breeding method that improves resistance to bacterial wilt mulberry tree output is characterized in that it carries out according to following steps in turn:
1) selects stock: choose the resistance to bacterial wilt kind and make stock anti-blue or green No. 10, prune stock;
2) select scion: choose human relations 40 good mulberries and do scion;
3) grafting: above-mentioned stock and scion are connect the grafting of method row according to bag, the intact film band of binding of grafting;
4) management: in time untie the film band during rudiment of fringe bar, bud is exposed, remove the anvil bud, to human relations 40 good mulberry normal growths.
2. the breeding method of raising resistance to bacterial wilt mulberry tree output according to claim 1 is characterized in that: prune the plant height 20-40cm of stock in the step 1), and keep the healthy and strong branch of 2-3 bar.
3. the breeding method of raising resistance to bacterial wilt mulberry tree output according to claim 1 is characterized in that: step 2) in scion select the full human relations of annual hibernaculum spore 40 good mulberry branchs.
4. the breeding method of raising resistance to bacterial wilt mulberry tree output according to claim 1, it is characterized in that: the selection of time of step 3) grafting is spring.
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| CN103329736B CN103329736B (en) | 2014-12-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931424A (en) * | 2014-03-21 | 2014-07-23 | 重庆市大足雅美佳水生花卉有限公司 | Fast cultivation method for good mulberry trees |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101796911A (en) * | 2010-03-21 | 2010-08-11 | 四川省丝绸科学研究院 | Mulberry panicle-sprout grafting-cuttage seedling raising method |
| CN102517376A (en) * | 2011-12-13 | 2012-06-27 | 广东省农业科学院蚕业与农产品加工研究所 | Method for identifying resistance to bacterial wilt disease of mulberry |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101796911A (en) * | 2010-03-21 | 2010-08-11 | 四川省丝绸科学研究院 | Mulberry panicle-sprout grafting-cuttage seedling raising method |
| CN102517376A (en) * | 2011-12-13 | 2012-06-27 | 广东省农业科学院蚕业与农产品加工研究所 | Method for identifying resistance to bacterial wilt disease of mulberry |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931424A (en) * | 2014-03-21 | 2014-07-23 | 重庆市大足雅美佳水生花卉有限公司 | Fast cultivation method for good mulberry trees |
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