CN103255088A - Magnetic flocculant and application method thereof - Google Patents

Magnetic flocculant and application method thereof Download PDF

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Publication number
CN103255088A
CN103255088A CN2013101570385A CN201310157038A CN103255088A CN 103255088 A CN103255088 A CN 103255088A CN 2013101570385 A CN2013101570385 A CN 2013101570385A CN 201310157038 A CN201310157038 A CN 201310157038A CN 103255088 A CN103255088 A CN 103255088A
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CN
China
Prior art keywords
magnetic
flocculant
magnetic flocculant
flocculation
ferric sulfate
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Application number
CN2013101570385A
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Chinese (zh)
Inventor
王新新
吴亮
杨勇
安伟
牛志刚
丁东华
Original Assignee
中国海洋石油总公司
中海油能源发展股份有限公司
中海石油环保服务(天津)有限公司
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Priority to CN2013101570385A priority Critical patent/CN103255088A/en
Publication of CN103255088A publication Critical patent/CN103255088A/en

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Abstract

The invention discloses a magnetic flocculant which consists of a flocculant and a magnetic seed, wherein the weight ratio of the flocculant to the magnetic seed is (1-100):(1-5). The invention also discloses an application method of the magnetic flocculant, which comprises the following steps of: adding the magnetic flocculant into fermentation liquid rich in petroleum hydrocarbon degrading thalli according to a weight ratio of 0.1-0.5%; uniformly stirring, and standing in a magnetic field; and after the substance to be shaped is condensed, separating out the flocculating body rich in petroleum hydrocarbon degrading thalli by use of separation equipment. According to the magnetic flocculant and the application method thereof disclosed by the invention, by taking the fermentation engineering as a theoretical basis and combining the related theoretical knowledge of the magnetic separation science and the environmental engineering, the fermentation liquid thalli are recovered by use of an Fe3O4-containing magnetic flocculant to solve the problem in flocculation recovery of the fermentation liquid thalli in the traditional technology; and the magnetic flocculant and the application method thereof have the advantages that the flocculation recovery is fast, the magnetic flocculant brings little influence on the activity of the microorganism thalli, and the magnetic seed in the magnetic flocculant can be repeatedly recovered and used.

Description

A kind of magnetic flocculant and application method thereof
Technical field
The invention belongs to the microbial fermentation technology field, particularly relate to a kind of magnetic flocculant and application method thereof.
Background technology
Adopting the microbial liquid fermentation process to obtain bulk petroleum alkane degradation bacterium is one of gordian technique of preparation petroleum hydrocarbon degradation microbial inoculum.Can effectively obtain a large amount of thalline by improving the fermented liquid cell density and improving its rate of recovery, so that follow-up microbial inoculum preparation work.Can effectively improve the fermented liquid cell density by optimal control fermention medium and fermentation condition.And the method for the rate of recovery of raising fermented liquid thalline is more, mainly contains: centrifuging, spray-drying process, filter press method and flocculence etc.Wherein the method investments such as centrifuging, spray-drying process, filter press method are large, equipment requirements is high.And the flocculence operation is comparatively simple, and is low to personnel and equipment requirements, thereby is widely used in actual production process.For example the patent No. is that 200910264752.8 Chinese patent discloses a kind of method that adopts Xylo-Mucine, cationic polyacrylamide and Triammonium citrate concentrated broth thalline; The patent No. is that 201080030690.5 Chinese patent discloses a kind of method that obtains target protein from fermented liquid flocculation bacterial cell.Yet traditional flocculation technique still need spend certain flocculation time, thereby has greatly limited the raising of microbial inoculum production rate.
The magnetic flocculation technology is a kind of rapidly and efficiently flocculation technique that grew up in the last few years.Add the part magnetic when adding flocculation agent, enhanced flocculation effect greatly, and obviously shorten flocculation time, and magnetic recyclable reusing have also greatly improved flocculation efficiency.At present, the research of magnetic flocculation technology aspect sewage disposal is used more.For example: the patent No. is that 201210115965.6 Chinese patent discloses a kind of magnetic powder flocculation agent and application method thereof.The magnetic powder of its use is with MFe 3O 4The spinel magnetic powder of expression.The patent No. is that 200810236222.8 Chinese patent discloses a kind of by nanometer Al 13With magnetic carrier SiO 2/ γ-Fe 3O 4-SiO 2Composite paramagnetic nano flocculant that forms and preparation method thereof; The patent No. is that 200510021449.7 Chinese patent discloses a kind of magnetic flocculation separation waste water treatment technology, can use water suspension≤20mg/L, oil-contg≤5mg/L.Thalline in the fermented liquid is present in the fermented liquid with the form of suspended substance, and is comparatively similar with the suspended substance in the sewage.Yet the research rare report of magnetic flocculation technology aspect the rate of recovery that improves the fermented liquid thalline, thereby this technology has larger application potential in this field.
Summary of the invention
The present invention provides a kind of magnetic flocculant and application method thereof of fast and effeciently concentrated broth thalline for solving the technical problem that exists in the known technology.
The technical scheme that the present invention takes for the technical problem that exists in the solution known technology is: a kind of magnetic flocculant, become to be grouped into by two kinds of flocculation agent and magnetic kinds, and the weight proportion of flocculation agent and magnetic kind is: 1~100:1~5.
The weight proportion of flocculation agent and magnetic kind is: 1~10:1~2.
Flocculation agent is the mixture of polymerize aluminum chloride and bodied ferric sulfate, and the weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1~100:1~100.
Flocculation agent is the mixture of polymerize aluminum chloride and bodied ferric sulfate, and the weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1~10:1~10.
The magnetic kind is that particle diameter is the Fe of 100nm~100um 3O 4Magnetic powder.
Another technical scheme that the present invention takes for the technical problem that exists in the solution known technology is: the application method of above-mentioned magnetic flocculant, described magnetic flocculant is joined in the fermented liquid that is rich in the petroleum hydrocarbon degradation bacterium thalline according to 0.1%~0.5% part by weight, in magnetic field, leave standstill after stirring, after the solid substance cohesion, adopt separating device that the flocs unit of enriching petroleun hydrocarbon degradation bacterium thalline is separated.
Described magnetic flocculant is joined in the fermented liquid that is rich in the petroleum hydrocarbon degradation bacterium thalline according to 0.2%~0.3% weight ratio.
Advantage and positively effect that the present invention has are: take fermentation engineering as theoretical foundation, the correlation theories knowledge of comprehensive magnetic separation science, environmental engineering for the coagulation and recovery problem of fermented liquid thalline in the traditional technology, adopts and contains Fe 3O 4Magnetic flocculant reclaim the fermented liquid thalline, have coagulation and recovery speed fast, magnetic flocculant is little to the microbial cells effect of vigor, the magnetic kind in the magnetic flocculant can reclaim the advantage of use repeatedly.
Embodiment
For further understanding summary of the invention of the present invention, Characteristic, hereby exemplify following examples, be described in detail as follows:
Embodiment 1:
A kind of magnetic flocculant is become to be grouped into by two kinds of flocculation agent and magnetic kinds, and the weight proportion of flocculation agent and magnetic kind is: 1:1; Wherein, flocculation agent is efficient flocculant, and the weight proportion of the preferably mixture of polymerize aluminum chloride and bodied ferric sulfate, and polymerize aluminum chloride and bodied ferric sulfate is: 1:1; The magnetic kind is that particle diameter is the Fe of 100nm~100um 3O 4Magnetic powder.
Above-mentioned magnetic flocculant is joined in the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Ornithinimicrobium kibberense W25 according to 0.2% weight ratio, in magnetic field, leave standstill after stirring, after the solid substance cohesion, adopt separating device that the flocs unit of enriching petroleun hydrocarbon degradation bacterium thalline is separated.
Add magnetic flocculant process in flocs unit maximum settlement speed can reach 1.4mm/s, behind the 10min in the flocs unit cell density can reach 1.7 * 10 9Cfu/ml improves 4.6 times than the fermented liquid without flocculation treatment, improves 4.2 times than the traditional technology of only adding the bodied ferric sulfate flocculation.
Embodiment 2:
Be with the difference of embodiment 1: the weight proportion of flocculation agent and magnetic kind is in the magnetic flocculant: 10:2; The weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 10:1.
Above-mentioned magnetic flocculant is joined in the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Ornithinimicrobium kibberense W25 according to 0.3% weight ratio.
Add magnetic flocculant process in flocs unit maximum settlement speed can reach 1.5mm/s, behind the 10min in the flocs unit cell density can reach 1.9 * 10 9Cfu/ml improves 5.1 times than the fermented liquid without flocculation treatment, improves 4.9 times than the traditional technology of only adding the bodied ferric sulfate flocculation.
Except foregoing, all the other contents of the present embodiment are all identical with embodiment 1.
Embodiment 3:
Be with the difference of embodiment 1: the weight proportion of flocculation agent and magnetic kind is in the magnetic flocculant: 100:5; The weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1:10.
Above-mentioned magnetic flocculant is joined in the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Ornithinimicrobiumkibberense W25 according to 0.1% weight ratio.
Add magnetic flocculant process in flocs unit maximum settlement speed can reach 1mm/s, behind the 10min in the flocs unit cell density can reach 1.3 * 10 9Cfu/ml improves 3.4 times than the fermented liquid without flocculation treatment, improves 3.2 times than the traditional technology of only adding the bodied ferric sulfate flocculation.
Except foregoing, all the other contents of the present embodiment are all identical with embodiment 1.
Embodiment 4:
Be with the difference of embodiment 1: the weight proportion of flocculation agent and magnetic kind is in the magnetic flocculant: 30:1; The weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 100:1.
Above-mentioned magnetic flocculant is joined in the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Ornithinimicrobiumkibberense W25 according to 0.5% weight ratio.
Add magnetic flocculant process in flocs unit maximum settlement speed can reach 0.9mm/s, behind the 10min in the flocs unit cell density can reach 1.2 * 10 9Cfu/ml improves 3.1 times than the fermented liquid without flocculation treatment, improves 3.0 times than the traditional technology of only adding the bodied ferric sulfate flocculation.
Except foregoing, all the other contents of the present embodiment are all identical with embodiment 1.
Embodiment 5:
Be with the difference of embodiment 1: the weight proportion of flocculation agent and magnetic kind is in the magnetic flocculant: 50:3; The weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1:100.
Above-mentioned magnetic flocculant is joined in the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Ornithinimicrobium kibberense W25 according to 0.4% weight ratio.
Add magnetic flocculant process in flocs unit maximum settlement speed can reach 1.2mm/s, behind the 10min in the flocs unit cell density can reach 1.5 * 10 9Cfu/ml improves 3.9 times than the fermented liquid without flocculation treatment, improves 3.7 times than the traditional technology of only adding the bodied ferric sulfate flocculation.
Except foregoing, all the other contents of the present embodiment are all identical with embodiment 1.
The fermented liquid that replaces being rich in above-described embodiment petroleum hydrocarbon degradation bacterium Ornithinimicrobium kibberense W25 bacterial classification with the fermented liquid that is rich in petroleum hydrocarbon degradation bacterium Rhodococcus jialingiae W7 bacterial classification adopts the effect of magnetic flocculant recovery petroleum hydrocarbon degradation bacterium provided by the invention close.

Claims (7)

1. a magnetic flocculant is characterized in that, is become to be grouped into by two kinds of flocculation agent and magnetic kinds, and the weight proportion of flocculation agent and magnetic kind is: 1~100:1~5.
2. magnetic flocculant according to claim 1 is characterized in that, the weight proportion of flocculation agent and magnetic kind is: 1~10:1~2.
3. magnetic flocculant according to claim 1 is characterized in that, flocculation agent is the mixture of polymerize aluminum chloride and bodied ferric sulfate, and the weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1~100:1~100.
4. magnetic flocculant according to claim 3 is characterized in that, flocculation agent is the mixture of polymerize aluminum chloride and bodied ferric sulfate, and the weight proportion of polymerize aluminum chloride and bodied ferric sulfate is: 1~10:1~10.
5. magnetic flocculant according to claim 1 is characterized in that, the magnetic kind is that particle diameter is the Fe of 100nm~100um 3O 4Magnetic powder.
6. the application method of magnetic flocculant as claimed in claim 1, it is characterized in that, described magnetic flocculant is joined in the fermented liquid that is rich in the petroleum hydrocarbon degradation bacterium thalline according to 0.1%~0.5% part by weight, in magnetic field, leave standstill after stirring, after the solid substance cohesion, adopt separating device that the flocs unit of enriching petroleun hydrocarbon degradation bacterium thalline is separated.
7. as according to method claimed in claim 6, it is characterized in that, described magnetic flocculant is joined in the fermented liquid that is rich in the petroleum hydrocarbon degradation bacterium thalline according to 0.2%~0.3% weight ratio.
CN2013101570385A 2013-04-28 2013-04-28 Magnetic flocculant and application method thereof CN103255088A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104211154A (en) * 2014-09-25 2014-12-17 王伟 Magnetic flocculating agent and application method thereof
CN106630059A (en) * 2016-12-14 2017-05-10 安徽吉思特智能装备有限公司 Magnetic powder composite polyferric sulfate flocculating agent and preparation method thereof
CN106966473A (en) * 2017-04-26 2017-07-21 环能科技股份有限公司 A kind of magnetic flocculant of aluminium salt load ferroso-ferric oxide and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101041530A (en) * 2007-03-11 2007-09-26 中国科学院等离子体物理研究所 Method and algaecide for treating algae bloom by strong-flocculation magnetic polymer
CN101665280A (en) * 2009-10-19 2010-03-10 大连理工大学 Method for waste water treatment and preparing magnetic flocculant by reclaimed floc
CN102775019A (en) * 2012-07-25 2012-11-14 华南理工大学 Coupling type sewage de-phosphorization purification regeneration treatment technique
CN103045579A (en) * 2012-12-17 2013-04-17 南开大学 Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101041530A (en) * 2007-03-11 2007-09-26 中国科学院等离子体物理研究所 Method and algaecide for treating algae bloom by strong-flocculation magnetic polymer
CN101665280A (en) * 2009-10-19 2010-03-10 大连理工大学 Method for waste water treatment and preparing magnetic flocculant by reclaimed floc
CN102775019A (en) * 2012-07-25 2012-11-14 华南理工大学 Coupling type sewage de-phosphorization purification regeneration treatment technique
CN103045579A (en) * 2012-12-17 2013-04-17 南开大学 Microbial remediation curing adsorbing bacterial preparation applicable to marine environment petroleum pollution as well as preparation method and application of same

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104211154A (en) * 2014-09-25 2014-12-17 王伟 Magnetic flocculating agent and application method thereof
CN106630059A (en) * 2016-12-14 2017-05-10 安徽吉思特智能装备有限公司 Magnetic powder composite polyferric sulfate flocculating agent and preparation method thereof
CN106966473A (en) * 2017-04-26 2017-07-21 环能科技股份有限公司 A kind of magnetic flocculant of aluminium salt load ferroso-ferric oxide and preparation method thereof

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