CN103252263A - Self-luminescence type micro-fluidic chip - Google Patents
Self-luminescence type micro-fluidic chip Download PDFInfo
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- CN103252263A CN103252263A CN2013102032182A CN201310203218A CN103252263A CN 103252263 A CN103252263 A CN 103252263A CN 2013102032182 A CN2013102032182 A CN 2013102032182A CN 201310203218 A CN201310203218 A CN 201310203218A CN 103252263 A CN103252263 A CN 103252263A
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Abstract
The invention provides a self-luminescence type micro-fluidic chip which realizes self-luminescence by means of internally integrating a chemical reaction agent. The chemical reaction agent in the chip is stored at a specific part in the micro-fluidic chip in a sealed liquid bag or cavity, and is prepared from three substances, namely peroxides, an ester compound and fluorescent dye. According to the working principle of the self-luminescence type micro-fluidic chip, the liquid bag is broken under an external action so as to release the chemical agent stored in the bag, then the released chemical agent is subjected to mixing reaction, and therefore, chemical luminescence is realized. In the self-luminescence way, external light source devices and complicated control equipment are not required; and the micro-fluidic chip is simple and convenient to operate and especially applicable to a portable micro total analysis system.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, particularly relate to a kind of emissive type micro-fluidic chip.
Background technology
In recent years, the micro-fluidic chip technology had obtained in fields such as biology, chemistry, medical science to study widely and use.Because in the application process of micro-fluidic chip, often many chemistry, biological reaction and detection all need carried out under specific light source illuminate condition.Therefore, light source control is extremely important in the application of micro-fluidic chip.
At present, to detect mainly be to rely on to add light source and realize for the light reaction of micro-fluidic chip or light.But this method has the following disadvantages: or need extra equipment and energy resource consumption, otherwise preparation technology is loaded down with trivial details and expensive.These weak points have all influenced the popularization of micro-fluidic chip, particularly at some instrument and equipment are required the micro-total analysis application of portability.By contrast, if can adopt the self-luminous pattern, and it is integrated on the micro-fluidic chip, thereby realization has very important and practical meanings to the light source control of micro-fluidic chip.As everyone knows, glo-stick has become very common a kind of article in people's life, and its principle is: be inserted in the glass tubule in the plastic tube that employing can be rolled over.In the process of fractureing, liquid in the glass tubule (different fluorescent pigments of all kinds and two oxalate diesters are dissolved in the solution of solvent) flows out, rapidly with plastic tube in liquid (hydrogen peroxide is dissolved in the solution of solvent) mix mutually, chemical reaction takes place, the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, fluorescent pigment releases energy (getting back to more stable lower state from upper state) with the form of visible light, thereby chemical energy is converted to luminous energy.On the platform that principle and the reagent system of glo-stick if can be moved on to micro-fluidic chip, can realize chemical light source integrated on micro-fluidic chip very easily, and realize self-luminous.
Summary of the invention
For achieving the above object, the invention provides a kind of emissive type micro-fluidic chip, this chip is to realize self-luminous by integrated chemical reaction reagent in micro-fluidic chip.
The invention provides a kind of emissive type micro-fluidic chip, the chemical reaction reagent of described chip internal is that the liquid scrotiform formula that seals is stored in ad-hoc location in the micro-fluidic chip.
The invention provides a kind of emissive type micro-fluidic chip, described micro-fluidic chip and liquid capsule material are one or several the combination wherein of dimethyl silicone polymer, polymethyl methacrylate, glass, polyethylene, polytetrafluoroethylene (PTFE), polyvinyl chloride, metal.
The invention provides a kind of emissive type micro-fluidic chip, described liquid capsule can be cylinder type, ball-type or rectangular build, the storage inside chemical reagent solution, and the outside is full-closed structure.
The invention provides a kind of emissive type micro-fluidic chip, the number of described liquid capsule is at least one, can be a plurality of liquid capsules of single liquid capsule internal package, also can be that a plurality of liquid capsules exist simultaneously.
The invention provides a kind of emissive type micro-fluidic chip, the chemical reagent of described liquid capsule storage inside is made up of peroxide, ester type compound and three kinds of materials of fluorescent dye.The chemical reagent of liquid capsule inside can separate independent storage, also can mix storage.But peroxide and lipoid substance must separately be stored.
The invention provides a kind of emissive type micro-fluidic chip, described peroxide preferably adopts hydrogen peroxide; Lipoid substance preferably uses two oxalate diesters; Fluorescent dye can be selected different fluorescers for use, comprises derivative, fluorescein of resorcinol phthalein, anthracene etc.
The invention provides a kind of emissive type micro-fluidic chip, the operation principle of described emissive type micro-fluidic chip is that effect makes the breakage of liquid capsule by external force, thereby the chemical reagent of storage inside is discharged and hybrid reaction, thereby realizes chemiluminescence.
The invention provides a kind of emissive type micro-fluidic chip, described external force refers to that the outside puts on the mechanicals efforts of liquid capsule, comprises extruding, knocks, vibrations, acupuncture etc.
The invention provides a kind of emissive type micro-fluidic chip, the number of described airtight cavity is at least two, adopts valve to isolate between the different cavitys.The operation principle of described cavity is, opens the separation valve door between the different cavitys, makes the different biochemical reactions of chemical reagent hybrid concurrency, thereby realizes chemiluminescence.
Use self-luminous method provided by the invention, need not to use external light source device and complicated control appliance, easy and simple to handle, be specially adapted to portable micro-total analysis system.
Description of drawings
Fig. 1. the structural representation of the chemical reagent storage area of a kind of emissive type micro-fluidic chip provided by the invention.Wherein, A is micro-fluidic chip; B is liquid capsule 1; C is liquid capsule 2; D is self luminous chemical light source.
Fig. 2. the emissive type microfluidic chip structure schematic cross-section in the embodiment of the invention two, wherein A ' is micro-fluidic chip, and B ' is liquid capsule 1, and C ' is liquid capsule 2, and D ' is liquid capsule 3.
Fig. 3. the emissive type microfluidic chip structure schematic cross-section in the embodiment of the invention three, wherein a is micro-fluidic chip, and b is liquid capsule 1, and c is liquid capsule 2.
Fig. 4. the emissive type microfluidic chip structure schematic cross-section in the embodiment of the invention four, wherein d is micro-fluidic chip, and e is liquid capsule 1, and f is liquid capsule 2, and g is liquid capsule 3.
Fig. 5. the structural representation of the emissive type micro-fluidic chip in the embodiment of the invention five, wherein h is micro-fluidic chip, and i is liquid capsule 1, and j is liquid capsule 2, and k is separation valve door.
Specific embodiments
The following examples will give further instruction to the present invention in conjunction with Figure of description.
1 one kinds of biliquid capsules of embodiment emissive type micro-fluidic chip
Fig. 1 is the structural representation of the liquid storage part of a kind of emissive type micro-fluidic chip provided by the invention, and the ad-hoc location among this micro-fluidic chip A has a bigger liquid capsule B, and inside is full of hydrogen peroxide solution; Other has a less liquid capsule C to be sealed among the liquid capsule B, is full of the mixed solution that fluorescent pigment and two oxalate diesters are arranged among the C.During use, rely on external force, make liquid capsule C breakage, thereby the liquid reagent that is stored in liquid capsule inside is released, with the reagent mix generation chemical reaction among the liquid capsule B, the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, and fluorescent pigment releases energy with the form of visible light, thereby chemical energy is converted to luminous energy D, realizes the self-luminous of chip.
2 one kind of three liquid capsule of embodiment emissive type micro-fluidic chip
Fig. 2 is the structural representation of the liquid storage part of a kind of emissive type micro-fluidic chip provided by the invention, and the ad-hoc location among this micro-fluidic chip A ' has a bigger liquid capsule B ', and inside is full of hydrogen peroxide solution; Other has less liquid capsule C ' and a D ' to be sealed among the liquid capsule B ', and being full of among the C ' has fluorescent pigment, and being full of among the D ' has two oxalate diester solution.During use, rely on external force, make liquid capsule C ' and D ' breakage, thereby the liquid reagent that is stored in liquid capsule inside is released, with the reagent mix generation chemical reaction among the liquid capsule B ', the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, and fluorescent pigment releases energy with the form of visible light, thereby chemical energy is converted to luminous energy, realizes the self-luminous of chip.
The spontaneous type micro-fluidic chip of 3 one kinds of parallel type biliquids of embodiment capsule
Fig. 3 is the structural representation of the liquid storage part of a kind of emissive type micro-fluidic chip provided by the invention, ad-hoc location among this micro-fluidic chip a has the liquid capsule b that inside is full of hydrogen peroxide solution, and another inside is full of the liquid capsule c of the mixed solution of fluorescent pigment and two oxalate diesters.During use, rely on external force, make liquid capsule b and c damaged simultaneously, thereby two kinds of liquid reagents that are stored in liquid capsule inside are released to mix chemical reaction take place, the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, fluorescent pigment releases energy with the form of visible light, thereby chemical energy is converted to luminous energy, realizes the self-luminous of chip.The spontaneous type micro-fluidic chip of 4 one kinds of parallel types of embodiment, three liquid capsules
Fig. 4 is the structural representation of the liquid storage part of a kind of emissive type micro-fluidic chip provided by the invention, ad-hoc location among this micro-fluidic chip d has the liquid capsule e that inside is full of hydrogen peroxide solution, an inside is full of the liquid capsule f of fluorescent pigment solution, and another inside is full of the liquid capsule g of two oxalate diester solution.During use, rely on external force, make liquid capsule e, f and g damaged simultaneously, thereby three kinds of liquid reagents that are stored in liquid capsule inside are released to mix chemical reaction take place, the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, fluorescent pigment releases energy with the form of visible light, thereby chemical energy is converted to luminous energy, realizes the self-luminous of chip.
The emissive type micro-fluidic chip of 5 one kinds of internal cavity storing reagents of embodiment
A kind of emissive type micro-fluidic chip h is prepared from by polymethyl methacrylate, and the structure at its self-luminous position stores the solution of two kinds of chemical reagent respectively as shown in Figure 5 in two airtight cavitys.Wherein, be sealed with hydrogen peroxide solution among the cavity i, be sealed with the mixed solution of fluorescent pigment and two oxalate diesters among the cavity j; Isolating valve, k by a screw-type between two cavitys keeps apart.During use, open isolating valve, k, thereby the liquid reagent that is stored in two inside cavity is released to mix chemical reaction takes place, the energy of emitting in chemical reaction passes to the fluorescent pigment molecule, fluorescent pigment releases energy with the form of visible light, thereby chemical energy is converted to luminous energy, realizes the self-luminous of chip.
Use self-luminous method provided by the invention, can and seal the liquid reagent that specifically has the self-luminous characteristic in the inner predetermined fixed of micro-fluidic chip, storage, by simple mechanical means, the release of handling liquids reagent at an easy rate, thereby the self-luminous on the realization micro-fluidic chip.The chemical light source that this is spontaneous need not externally fed, and by regulating the kind of fluorescent dye, can obtain the illumination effect of different wave length, has extremely important practical significance in the analyzing and testing field of micro-fluidic chip.
Claims (10)
1. an emissive type micro-fluidic chip is characterized in that this micro-fluidic chip is by realizing self-luminous at chip internal integrated chemical reaction reagent.
2. according to the described a kind of emissive type micro-fluidic chip of claim 1, it is characterized in that the chemical reaction reagent of described chip internal is that the liquid capsule that seals or cavity form are stored in ad-hoc location in the micro-fluidic chip.
3. according to claim 1 and 2 described a kind of emissive type micro-fluidic chips, it is characterized in that described micro-fluidic chip and liquid capsule material are one or several the combination wherein of dimethyl silicone polymer, polymethyl methacrylate, glass, polyethylene, polytetrafluoroethylene (PTFE), polyvinyl chloride, metal.
4. according to the described a kind of emissive type micro-fluidic chip of claim 2, it is characterized in that the number of described liquid capsule is at least one, can be a plurality of liquid capsules of single liquid capsule internal package, also can be that a plurality of liquid capsules exist simultaneously.
5. according to the described a kind of emissive type micro-fluidic chip of claim 2, it is characterized in that the chemical reagent of described liquid capsule storage inside is made up of peroxide, ester type compound and three kinds of materials of fluorescent dye.
6. according to claim 2 and 6 described a kind of emissive type micro-fluidic chips, it is characterized in that the chemical reagent of described liquid capsule inside can separate independent storage, also can mix storage.But peroxide and lipoid substance must separately be stored.
7. according to claim 2 and 6 described a kind of emissive type micro-fluidic chips, it is characterized in that described peroxide preferably adopts hydrogen peroxide; Lipoid substance preferably uses two oxalate diesters; Fluorescent dye can be selected different fluorescers for use, comprises derivative, fluorescein of resorcinol phthalein, anthracene etc.
8. according to the described a kind of emissive type micro-fluidic chip of claim 1, it is characterized in that the operation principle of described emissive type micro-fluidic chip is that effect makes the breakage of liquid capsule by external force, thereby the chemical reagent of storage inside is discharged and hybrid reaction, thereby realize chemiluminescence.
9. according to the described a kind of emissive type micro-fluidic chip of claim 9, it is characterized in that described external force refers to that the outside puts on the mechanicals efforts of liquid capsule, comprise extruding, knock, vibrations, acupuncture etc.
10. according to the described a kind of emissive type micro-fluidic chip of claim 1, it is characterized in that, use self-luminous method provided by the invention, need not to use external light source device and complicated control appliance, easy and simple to handle, be specially adapted to portable micro-total analysis system.
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| CN2013102032182A CN103252263A (en) | 2013-05-27 | 2013-05-27 | Self-luminescence type micro-fluidic chip |
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| CN2013102032182A CN103252263A (en) | 2013-05-27 | 2013-05-27 | Self-luminescence type micro-fluidic chip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109420531A (en) * | 2017-08-31 | 2019-03-05 | 厦门大学 | The storage of micro-fluidic chip and its reagent and method for releasing |
| CN110470599A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Micro-total analysis chip and reaction in-situ method |
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| US5731212A (en) * | 1994-12-20 | 1998-03-24 | International Technidyne Corporation | Test apparatus and method for testing cuvette accommodated samples |
| CN101692047A (en) * | 2009-10-27 | 2010-04-07 | 浙江大学 | Microfluidic chip for capillary electrophoresis separation and chemiluminescence detection |
| CN102644049A (en) * | 2012-04-26 | 2012-08-22 | 北京工业大学 | Micro-flow driving method based on TiO2 nano-film wettability |
| CN202725200U (en) * | 2012-07-20 | 2013-02-13 | 广东药学院 | Liquid storage groove in micro-fluidic chip |
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2013
- 2013-05-27 CN CN2013102032182A patent/CN103252263A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5731212A (en) * | 1994-12-20 | 1998-03-24 | International Technidyne Corporation | Test apparatus and method for testing cuvette accommodated samples |
| CN101692047A (en) * | 2009-10-27 | 2010-04-07 | 浙江大学 | Microfluidic chip for capillary electrophoresis separation and chemiluminescence detection |
| CN102644049A (en) * | 2012-04-26 | 2012-08-22 | 北京工业大学 | Micro-flow driving method based on TiO2 nano-film wettability |
| CN202725200U (en) * | 2012-07-20 | 2013-02-13 | 广东药学院 | Liquid storage groove in micro-fluidic chip |
Non-Patent Citations (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109420531A (en) * | 2017-08-31 | 2019-03-05 | 厦门大学 | The storage of micro-fluidic chip and its reagent and method for releasing |
| CN110470599A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Micro-total analysis chip and reaction in-situ method |
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Application publication date: 20130821 |