CN103238543B - Method for controlling large algae in sea cucumber pond on basis of biotechnology - Google Patents

Method for controlling large algae in sea cucumber pond on basis of biotechnology Download PDF

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Publication number
CN103238543B
CN103238543B CN201310188836.4A CN201310188836A CN103238543B CN 103238543 B CN103238543 B CN 103238543B CN 201310188836 A CN201310188836 A CN 201310188836A CN 103238543 B CN103238543 B CN 103238543B
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pond
macro
transparency
fertilizer
water
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CN103238543A (en
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刘彤
李勃
宋晓阳
陈文博
张澎
金文鑫
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DALIAN AQUATIC PRODUCT TECHNOLOGY PROMOTION MASTER STATION
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DALIAN AQUATIC PRODUCT TECHNOLOGY PROMOTION MASTER STATION
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a method for controlling large algae in a sea cucumber pond on the basis of biotechnology. After water temperature rises to 5 DEG C for the first time after early spring and when the large algae grow in the pond or pond transparency is larger than 80cm, bacillus subtilis is evenly sprinkled to the water surface of the pond with the large algae, the microbial content per square meter is 1.5-2.5 billion, diatom breeding fertilizer and fermentation organic fertilizer for promoting bacillus subtilis growth and lowering transparency are evenly sprinkled, so that the pond transparency is lowered to 80cm, and restraint of the large algae is achieved. According to the biological method, the relative transparency is adjusted through alga introduction and matched using of specific inorganic fertilizer, organic fertilizer and bacillus subtilis according to growth laws of the large algae in ponds of different areas at different time nodes so as to restrain photosynthesis of the large algae, and accordingly the goals of controlling rampant growth of the algae and not influencing normal growth of sea cucumbers are achieved.

Description

Based on the method for biotechnology control holothurian pond macro
Technical field
The present invention relates to holothruian cultures technical field, more particularly, relate to the especially control method of macro of holothurian pond breeding environment.
background technology
Macro spreads unchecked the harm that holothruian cultures are caused, and is to make in recent years the very problem of headache of culturist, and enterprise or cultivation producer kill by artificial Salvage and Wreck Removal or with weed killer herbicide conventionally, and not only cost height easily pollutes, and controls poor effect.
Algae normal growth must possess three conditions: spore, nutrition, illumination.
summary of the invention
The present invention controls macro growth by regulating illumination, is intended to solve the negative effect of the medium-and-large-sized algae of holothruian cultures, by low-cost processes method, thereby reaches the object that improves holothruian cultures benefit.It is the holothurian cultivation pond of silt or husky mud character that the present invention is applicable to 30~150 mu, ground, the bottom material of pond for breeding such as Liaoning, Shandong and Hebei, and these holothurian cultivation ponds monthly can change to a certain amount of outer seawater.
In order to achieve the above object, the invention provides a kind of method based on biotechnology control holothurian pond macro, it is characterized in that, after early spring, water temperature rises first and reaches after 5 DEG C, rose to first before 15 DEG C to water temperature, pond finds that there is macro growth, to the pond water surface that the has macro bacillus subtilis of evenly splashing, 15~2,500,000,000 of every square meter bacterium numbers, and coordinate the diatom of evenly splashing to breed fertilizer and promote the fermentation fertilizer of bacillus subtilis bacteria growing, reduction transparency, realize the inhibition of macro.
Wherein, described macro is Enteromorpha, bristle algae, bristle algae or sea lettuce.Under optimal way, the method of bacillus subtilis and fertilizer of splashing is: wind speed is less than in the situation of 3 grades, throw in bacillus subtilis bacterium powder, specifically use 800~1500g fermentation fertilizer, 15~25 grams, the bacillus subtilis bacterium powder of 10,000,000,000/g, 4L water, 50~70g nutriment according to the algae area of every 100 square metres, stir; With evenly splashing to pond, the place water surface that has macro after the dilution of 20L water, allow it naturally sink to the bottom, after splashing 3~5 days, change again water; Throw in second day after bacillus subtilis, throw in diatom breeding fertilizer 1kg/ mu.
Under optimal way, the mode of producing of described fermentation fertilizer is: animal wastes, add the high-carbon thing powder of about 30-70%, with yeast or lactobacillus-fermented to till producing white hypha; Described nutriment is brown sugar, white sugar or glucose.
In addition, the present invention is based on the method for biotechnology control holothurian pond macro, optimum way, melts and begins to control holothurian pond from pond ice, and concrete steps are:
After S1, ice, change 20%~30% pond water into outer seawater, thereby the phytoplankton of introducing Ying Ji is as provenance, appends diatom breeding fertilizer and cultivates phytoplankton;
S2, early spring, water temperature rises first and reaches after 5 DEG C, and holothurian pond transparency is less than 80 centimetres and do not find in the situation of macro growth, by changing water and using the chlorine dioxide of 1~3ppm to kill part phytoplankton, strengthens transparency to 80cm;
S3, maintain transparency 80cm, rise to first 15 DEG C to water temperature;
S4, spring after this, see through and throw in fermentation fertilizer and diatom breeding fertilizer, makes pond transparency be down to 20-40cm; After this, execute fermentation fertilizer and diatom breeding fertilizer by changing water, input bacillus subtilis and benefit, pond transparency 20-40cm is remained to and finish summer.
Under optimal way, in step S1, change after water, throw in low form bacillus subtilis formulation 400~800g/ mu of suitable 4~15 DEG C of growths of 10,000,000,000/g simultaneously; And the input dry fertilizer 3~6kg/ mu of fermentation and the fertile 1~2kg/ mu of diatom breeding.
The present invention adopts biological method, at different time node, according to the growth rhythm of the medium-and-large-sized algae in pond, different regions, collocation by the introducing of algae kind, specific inorganic fertilizer, fertilizer and bacillus subtilis is used, regulate and control relative transparency (relative value changing along with season, depth of water and water temperature change), suppress macro photosynthesis, control thereby reach the object that algae is spread unchecked growth and do not affect sea cucumber normal growth.
Embodiment
The present invention is based on the method for biotechnology control holothurian pond macro, optimum way just starts to control holothurian cultivation pond from iceization later, and detailed process is as follows:
After holothurian pond ice, topdress immediately and cultivate basic phytoplankton.The macro of holothurian cultivation pond is generally to overflow in the time of high temperature.And the work of controlling macro will start in the time that holothurian cultivation pond is just changed ice.First, after holothurian pond ice, pond waters is generally more clear, at the bottom of can clearly seeing pond, at this moment needs to change part (20%~30%) holothurian pond water into outer seawater, is brought into and should be answered the phytoplankton on ground as provenance (or artificially throwing in) in season by outer seawater.Under optimal way, throw in low form bacillus subtilis formulation (suitable about 4~15 DEG C growths), supply volume is preferably: 10,000,000,000/g bacillus subtilis powder is thrown in 400g~800g/ mu.And the input dry fertilizer 3~6kg/ mu of fermentation and fertile cream 1~2kg/ mu (, diatom breeding fertilizer).Rich water can make phytoplankton keep a suitable density and vigorous growth conditions, turns out good water colour and transparency, lays good basis for controlling macro.Probiotics (bacillus subtilis) improves bottom environment can increase benthic diatom breeding, lays the foundation for holothruian cultures improve output.
Early spring, water temperature reaches first 5 DEG C and starts management later, requirement makes the absolute value of holothurian pond transparency be less than 80 centimetres, just need to be by changing water, mending and execute probiotics (bacillus subtilis) and topdress and make pond seawater fertilizer if transparency is obviously greater than 80 centimetres, transparency drops to 80 centimetres of standard-requireds.If transparency is significantly less than 80 centimetres, need to change water and use chlorine dioxide (1~3ppm) to kill part phytoplankton, transparency is strengthened, be transferred to the standard-required of 80 centimetres.
During this, to, along with the variation of transparency and the pond depth of water, by changing water, mending and execute probiotics and topdress, make water colour and transparency reach above-mentioned requirements respectively.This just requires holothruian cultures person to accomplish to patrol the pool every day, and patrolling one of the important content on the pool is to observe water colour and transparency.The minor variations of water colour and transparency must be accomplished timely discovery, and early detection regulate and control method is very simple, and flower cost is little, and it is fast that water colour and transparency are recovered.On the contrary, process not in time, when in pond, transparency continues suddenly increasing or muddiness, make up again, need certain hour could recover original water colour and transparency, spent money also may not produce effect, loss may be very large, even causes the failure of regulation and control, and then cause holothruian cultures to receive impact.
Keep spring (3~15 DEG C of water temperatures) normally changing water, transparency is slightly shown in that the standard at the bottom of pond appreciates 15 DEG C first until be maintained to water temperature relatively.After this, if do not have macro to occur in pond, just directly strengthen fertilising, throw in fermentation fertilizer 6~10kg/ mu and diatom breeding fertilizer 2kg/ mu (can suitably increase and decrease according to former pond girth of a garment situation), original transparency is reduced rapidly, be reduced to transparency always and be less than pond beach face depth of water 30cm left and right (20~40 centimetres).Reach after above-mentioned transparency, daily management is still by changing water, mending and execute probiotics and topdress, make pond water colour and transparency keep above-mentioned requirements until finish summer, be limited can not normal growth because of growth primary condition can to ensure like this macro, reaches the object of control.
If not control well early spring, have been found that macro (Enteromorpha, bristle algae, bristle algae, sea lettuce etc.) starts growth, change after water at large flood tide, first throw in bacillus subtilis, concrete injected volume determines according to algae distribution area in pond.After this time period is generally early spring, water temperature rises first and reaches after 5 DEG C, rose to first before 15 DEG C to water temperature, or water temperature rise first reach 5 DEG C after to time periods in summer.
Preferably input mode of a kind of bacillus subtilis is: with the bacillus subtilis bacterium powder of 10,000,000,000/g, select before 9 of fine weather (wind speed is less than 3 grades) mornings, use 800~1500g fermentation fertilizer+15~25g bacillus subtilis bacterium powder+4L water+50~70g brown sugar according to the algae areas of every 100 square metres, stir at suitable vessel (as management ship), mix thoroughly latter 2 hours, again with the dilution of 20L water, dilution is evenly splashed to pond, the place water surface that has macro as far as possible, allow it naturally sink to the bottom, within after splashing 3~5 days, try not to change water.Throw in second day after bacillus subtilis, then throw in diatom breeding fertilizer 1kg/ mu.After 10 days, various macros can be because bacillus subtilis be in the suppressed growth of frond surface amount reproduction, and the phytoplankton in pond can, because of the rapid breeding of providing of fertilizer, obviously reduce transparency simultaneously.The reduction of transparency can make macro because of the not enough death gradually of illumination, dead algae is decomposed and becomes available fertilizer by bacillus subtilis again, continuing as phytoplankton provides battalion suitable nutritive salt, promotes unicellular alga Growth and Reproduction, keeps conveniently transparency.Bacillus subtilis self rapidly breeding, for dominant bacteria, can also suppress growing of holothurian pond pathogenic microorganism.
Follow that this is reciprocal, form an optimum ecological circulation, make the bacterium phase of holothurian cultivation pond reach mutually balance with algae.Maintain metastable water colour and transparency, restrained macro growth, return sea cucumber sufficient benthic diatom is provided, for good ecotope is built in sea cucumber growth.
Above-mentioned implementation process is particularly useful for the holothruian cultures under the weather conditions of Dalian.By said process, in practice, the present invention is based on the method for biotechnology control holothurian pond macro, can suppress the breeding of macro in pond.
In addition, in above-mentioned embodiment, the procurement process of fermentation fertilizer can be obtained by purchase, also can obtain by self-control, concrete self-control process is: animal wastes are as chicken manure, pig manure or stalk, chicken manure adds the high-carbon material of about 30-70%, for example sawdust, wooden chaff, manioc waste, bagasse, powder of straw, peanut hull meal, powdered rice hulls, tendril crushed material, dry, powdery, high-carbon.Also can directly use manure fermentation (yeast or lactic acid bacteria), in heap, producing white hypha can use.
And above-mentioned embodiment, diatom breeding is fertile, for promoting the growth of the phytoplanktons such as diatom, to promote transparency obviously to reduce, and makes macro because of the death gradually of illumination deficiency; Its supply volume is to keep pond transparency respective standard to be advisable.
Fermentation fertilizer and diatom breed fertile supply volume and increase and decrease as required.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; any be familiar with those skilled in the art the present invention disclose technical scope in; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.

Claims (5)

1. the method based on biotechnology control holothurian pond macro, it is characterized in that, after early spring, water temperature rises first and reaches after 5 DEG C, rises to first before 15 DEG C to water temperature, and pond finds that there is macro growth, to the pond water surface that the has macro bacillus subtilis of evenly splashing, 15~2,500,000,000 of every square meter bacterium numbers, and coordinate the diatom of evenly splashing to breed fertilizer and promote the fermentation fertilizer of bacillus subtilis bacteria growing, reduction transparency, realize the inhibition of macro.
2. the method based on biotechnology control holothurian pond macro according to claim 1, is characterized in that, described macro is Enteromorpha, bristle algae, bristle algae or sea lettuce.
3. the method based on biotechnology control holothurian pond macro according to claim 2, is characterized in that, the method for splash bacillus subtilis and fertilizer is:
Wind speed is less than in the situation of 3 grades, throw in bacillus subtilis bacterium powder, specifically use 800~1500g fermentation fertilizer, 15~25 grams, the bacillus subtilis bacterium powder of 10,000,000,000/g, 4L water, 50~70g nutriment according to the algae area of every 100 square metres, stir; With evenly splashing to pond, the place water surface that has macro after the dilution of 20L water, allow it naturally sink to the bottom, after splashing 3~5 days, change again water;
Throw in second day after bacillus subtilis, throw in diatom breeding fertilizer 1kg/ mu.
4. the method based on biotechnology control holothurian pond macro according to claim 3, is characterized in that,
The mode of producing of described fermentation fertilizer is: animal wastes, add the high-carbon thing powder of 30-70%, with yeast or lactobacillus-fermented to till producing white hypha;
Described nutriment is brown sugar, white sugar or glucose.
5. according to the arbitrary described method based on biotechnology control holothurian pond macro of claim 1-4, it is characterized in that, melt and begin to control holothurian pond from pond ice, concrete steps are:
After S1, ice, change 20%~30% pond water into outer seawater, thereby the phytoplankton of introducing Ying Ji is as provenance, appends diatom breeding fertilizer and cultivates phytoplankton;
S2, early spring, water temperature rises first and reaches after 5 DEG C, and holothurian pond transparency is less than 80 centimetres and do not find in the situation of macro growth, by changing water and using the chlorine dioxide of 1~3ppm to kill part phytoplankton, strengthens transparency to 80cm;
S3, maintain transparency 80cm, rise to first 15 DEG C to water temperature;
S4, spring after this, see through and throw in fermentation fertilizer and diatom breeding fertilizer, makes pond transparency be down to 20-40cm; After this, execute fermentation fertilizer and diatom breeding fertilizer by changing water, input bacillus subtilis and benefit, pond transparency 20-40cm is remained to and finish summer.
CN201310188836.4A 2013-05-20 2013-05-20 Method for controlling large algae in sea cucumber pond on basis of biotechnology Expired - Fee Related CN103238543B (en)

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CN103598080A (en) * 2013-12-09 2014-02-26 文登市水产技术推广站 Method for controlling macroalgaes in stichopus japonicas culture pond
CN104328075B (en) * 2014-11-06 2017-02-15 山东宝来利来生物工程股份有限公司 Bacillus subtilis strain capable of killing algae and application thereof
CN105075952B (en) * 2015-09-09 2018-07-31 大连市水产技术推广总站 Using the method for Copepods during the net cage seedling nursery of biological prevention and control stichopus japonicus sea area
CN105585135A (en) * 2015-12-23 2016-05-18 天津荣利生物科技发展有限公司 Method for controlling cyanobacteria in aquaculture water
CN106905013A (en) * 2017-02-27 2017-06-30 威海市四合生物科技有限公司 The production method of fermented type element silicon composite fertilizer
CN108651340A (en) * 2018-04-17 2018-10-16 烟台市水产研究所 A kind of ecological preventing control method of apostichopus japonicus culture pond macro excessive multiplication

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