CN103217326B - Sample reaction cabin and detection reagent adding method - Google Patents
Sample reaction cabin and detection reagent adding method Download PDFInfo
- Publication number
- CN103217326B CN103217326B CN201310105128.XA CN201310105128A CN103217326B CN 103217326 B CN103217326 B CN 103217326B CN 201310105128 A CN201310105128 A CN 201310105128A CN 103217326 B CN103217326 B CN 103217326B
- Authority
- CN
- China
- Prior art keywords
- slide
- cylinder
- upper cover
- cover
- pilot hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a sample reaction cabin and a detection reagent adding method. The sample reaction cabin comprises an upper cover and a lower cover which are fixed together in a detachable connection manner; a slide is arranged between the upper cover and the lower cover, and the upper part of the upper cover is provided with a cylinder; the cylinder comprises an upper opening in the top end and a lower opening in the lower surface of the upper cover, the lower cover is provided with a locating hole, the diameter of the locating hole is smaller than the inner diameter of the cylinder, and the projection area of the locating hole in the slide is in the projection area of the lower opening in the cylinder on the slide. In comparison with the prior art, the sample reaction cabin and the detection reagent adding method provided by the invention can accurately determine the positions of the tissue slices and cell smears by moving the slide in the detection process, subsequently can directly add the reagent needed for detection through locating by a machine, and thus, the adding process of the reagent is simplified, more obviously, dosage of the reagent can be reduced greatly, and the detection cost is saved.
Description
Technical field
The present invention relates to a kind of preparation facilities of cell detection sample, especially a kind of example reaction storehouse and detection Reagent adding methods.
Background technology
Cell sample needs to observe after dyeing, detect, when carrying out cell dyeing, needs are by dyeing storehouse, existing dyeing storehouse normally comprises a cylinder and a slide be placed in below cylinder lower openings, when adding coloring agent, first cell sample is placed on slide, move slide again, cell sample is made to be in cylinder lower openings on slide in view field, then coloring agent is added from the upper shed of cylinder, coloring agent is made to cover the view field of cylinder lower openings on slide completely, so just can ensure that coloring agent covers cell sample completely, ensure that coloring agent and cell sample fully react, finally obtain testing result accurately when detecting.
Existing dyeing storehouse is owing to cannot determine the position of cell sample, namely cannot determine cell sample is in which position of above-mentioned view field accurately, just need to add more coloring agent, make coloring agent cover above-mentioned view field completely, under normal circumstances, coloring agent costly, this just makes the cost of cell dyeing very high, if control cost, when reducing the addition of coloring agent, very likely not reach the metering that reaction requires.
Summary of the invention
For the deficiencies in the prior art, an object of the present invention, in providing a kind of example reaction storehouse, is ensureing that under the prerequisite that reaction is normally carried out, that can reduce coloring agent greatly adds metering.
Two of object of the present invention is that providing a kind of detects Reagent adding methods, is ensureing that under the prerequisite that reaction is normally carried out, that can reduce coloring agent greatly adds metering.
For achieving the above object one, the present invention adopts following technical scheme:
Example reaction storehouse, the upper cover that the mode comprising removably connecting is fixed together and lower cover, a slide is provided with between upper cover and lower cover, upper cover top is provided with a cylinder, this cylinder has the upper shed being positioned at its top and the under shed being positioned at upper cover lower surface, under cover and be provided with a pilot hole, the diameter of this pilot hole is less than the internal diameter of cylinder, and the view field that this pilot hole is formed on slide is positioned at the view field that cylinder lower openings is formed on slide.
The central shaft of pilot hole overlaps with the central shaft of cylinder.
The edge of cylinder upper shed is also provided with a flow-guiding mouth.
An O-ring seal is provided with between the periphery of under shed and the upper surface of slide.
Upper cover is connected in the mode fastened with lower cover.
The side of lower cover is provided with a hanging hole, the side that upper cover is corresponding with hanging hole is provided with the mounting flange that penetrates hanging hole, the opposite side of lower cover is provided with a hanging platform, the side that upper cover is corresponding with hanging platform is provided with a mounting groove penetrated for hanging platform, and the lateral surface of hanging platform is in the inclined-plane tilted to the inside gradually from the bottom to top.
For achieving the above object two, the present invention adopts following technical scheme:
Detect Reagent adding methods, comprise the steps:
Steps A, get a slide, cell sample to be dyed is placed on slide;
Step B, the slide carrying cell sample is positioned over one covers with under pilot hole, mobile slide, makes cell sample to be dyed and pilot hole consistency from top to bottom;
Step C, get the upper cover that a top is provided with cylinder, the lower openings of cylinder is positioned at the lower surface of upper cover, the diameter of pilot hole is less than the internal diameter of cylinder, upper cover is fixed on down and covers, make slide between upper cover and lower cover, and ensure that the view field of pilot hole on slide is positioned at the view field of cylinder lower openings on slide; The accurate location of cylinder lower openings view field on slide is positioned at by the projected position determination cell sample of pilot hole on slide;
Step D, mobile coloring agent instillation device, makes the liquid outlet of instillation device be positioned at cylinder upper opening place and is positioned at directly over the above-mentioned position determined, and instillation coloring agent, makes coloring agent drop on slide and cover cell sample.
In above-mentioned steps C, the central axis of pilot hole and the central axes of cylinder.
Beneficial effect of the present invention is:
Compared to prior art, example reaction storehouse of the present invention and detection Reagent adding methods, can in testing process, by mobile slide, determine the position residing for histotomy, cell smear accurately, thus can directly locate by machine the reagent added needed for detection, simplify the adition process of reagent, more be apparent that, the consumption of reagent can be reduced greatly, save testing cost.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 scheme of installation of the present invention;
Wherein: 10, upper cover; 11, flange is mounted; 12, groove is mounted; 20, lower cover; 21, hanging hole; 22, hanging platform; 23, pilot hole; 30, cylinder; 31, upper shed; 32, flow-guiding mouth; 40, slide; 50, O-ring seal.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
See Fig. 1, 2, example reaction storehouse of the present invention comprises upper cover 10, lower cover 20, cylinder 30, slide 40, upper cover 10 and lower cover 20 are fixed together in the mode fastened, cylinder 30 is positioned at the top of upper cover 10, it can be and upper cover 10 integrated injection molding, cylinder 30 has the upper shed 31 being positioned at its top, this upper shed 31 is for observing and instillation coloring agent, the lower openings (not shown) of cylinder 30 is positioned at the lower surface of upper cover 10, that is, the cylinder chamber of cylinder 30 is through to the top of slide 40 by its upper shed 31, slide 40 can be observed directly from the upper shed 31 of cylinder 30.Lower cover 20 has a pilot hole 23, this pilot hole 23 can be the center being opened in lower cover 20, can certainly be opened in other suitable positions, when the slide 40 of carrying cell sample is positioned on lower cover 20, by mobile slide 40, can by the cell sample that makes on slide 40 and pilot hole 23 consistency from top to bottom.After upper cover 10 and lower cover 20 are fastened, the view field of pilot hole 23 on slide 40 is positioned at the view field of lower openings on slide 40 of cylinder 30, like this, just can learn that cell sample is positioned at the accurate location of cylinder 30 lower openings in the view field of slide 40, so, when adding coloring agent, the liquid outlet that just coloring agent can be instilled device is placed in the corresponding position of cylinder 30 upper shed 31, and coloring agent is from top to bottom dripped on cell sample accurately.
For the ease of operation, the central shaft of above-mentioned pilot hole 23 can be made to overlap with the central shaft of cylinder 30, so just can ensure that cell sample is on the central axis of cylinder 30 all the time, when adding coloring agent, the liquid outlet directly coloring agent being instilled device moves to the middle position of cylinder 30 upper opening.
For the ease of collecting reacted liquid, can arrange a flow-guiding mouth 32 at the edge of the upper shed 31 of above-mentioned cylinder 30, in the completed, the whole device of tiltable, this flow-guiding mouth 32 of being postponed by reacted liquid is poured into collection container.
Be provided with an O-ring seal 50 between the under shed periphery of above-mentioned cylinder 30 and the upper surface of slide 40, sealing circle 50 is mainly used in encapsulating the view field of cylinder 30 lower openings on slide 40, avoids coloring agent to flow out.O-ring seal 50 can also play buffer action simultaneously, and what solution slide 40 variable thickness caused is pressed from both sides bad or not clamping problem by upper cover 10 and lower cover 20.
The manner of upper cover 10 of the present invention and lower cover 20 particularly, the side of lower cover 20 is provided with a hanging hole 21, the side that upper cover 10 is corresponding with hanging hole 21 is provided with the mounting flange 11 that penetrates hanging hole 21, the opposite side of lower cover 20 is provided with a hanging platform 22, the side that upper cover 10 is corresponding with hanging platform 22 is provided with a mounting groove 12 penetrated for hanging platform 22, and the lateral surface of hanging platform 22 is in the inclined-plane tilted to the inside gradually from the bottom to top.When upper cover 10 is fixed with lower cover 20, first penetrate in hanging hole 21 by mounting flange 11, then press down hanging platform 22, hanging platform 22 penetrates into after there is elastic deformation in mounting groove 12, thus upper cover 10 and lower cover 20 are fixed.Certainly, upper cover 10 of the present invention and lower cover 20 also can adopt other the mode of removably connecting to be fixed, and do not do illustrating one by one here.
In the present invention, lower cover 20 can adopt metal material, thus forms heat conductor, directly heats slide 40, is in required temperature range to make reaction.
Detection Reagent adding methods of the present invention, its concrete steps are as follows:
Steps A, get a slide 40, cell sample to be dyed is placed on slide 40;
Step B, the slide 40 carrying cell sample is positioned over one with on the lower cover 20 of pilot hole 23, mobile slide 40, makes cell sample to be dyed and pilot hole 23 consistency from top to bottom;
Step C, get the upper cover 10 that a top is provided with cylinder 30, the lower openings of cylinder 30 is positioned at the lower surface of upper cover 10, the diameter of pilot hole 23 is less than the internal diameter of cylinder 30, upper cover 10 is fixed on lower cover 20, make slide 40 between upper cover 10 and lower cover 20, and ensure that the view field of pilot hole 23 on slide 40 is positioned at the view field of cylinder 30 lower openings on slide 40; The accurate location of cylinder 30 lower openings view field on slide 40 is positioned at by the projected position determination cell sample of pilot hole 23 on slide 40; In this step, conveniently the adding of coloring agent in subsequent step, the central shaft of cylinder 30 can be made to overlap with the central shaft of pilot hole 23;
Step D, mobile coloring agent instillation device, makes the liquid outlet of instillation device be positioned at the center of cylinder 30 upper shed 31, and instillation coloring agent, makes coloring agent drop on slide 40 and cover cell sample.
To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the claims in the present invention.
Claims (8)
1. example reaction storehouse, it is characterized in that, the upper cover that the mode comprising removably connecting is fixed together and lower cover, a slide is provided with between upper cover and lower cover, upper cover top is provided with a cylinder, and this cylinder has the upper shed being positioned at its top and the under shed being positioned at upper cover lower surface, under cover and be provided with a pilot hole, the diameter of this pilot hole is less than the internal diameter of cylinder, and the view field that this pilot hole is formed on slide is positioned at the view field that cylinder lower openings is formed on slide.
2. example reaction storehouse as claimed in claim 1, it is characterized in that, the central shaft of pilot hole overlaps with the central shaft of cylinder.
3. example reaction storehouse as claimed in claim 1, it is characterized in that, the edge of cylinder upper shed is also provided with a flow-guiding mouth.
4. example reaction storehouse as claimed in claim 1, is characterized in that, be provided with an O-ring seal between the periphery of under shed and the upper surface of slide.
5. example reaction storehouse as claimed in claim 1, is characterized in that, upper cover is connected in the mode fastened with lower cover.
6. example reaction storehouse as claimed in claim 5, it is characterized in that, the side of lower cover is provided with a hanging hole, the side that upper cover is corresponding with hanging hole is provided with the mounting flange that penetrates hanging hole, the opposite side of lower cover is provided with a hanging platform, the side that upper cover is corresponding with hanging platform is provided with a mounting groove penetrated for hanging platform, and the lateral surface of hanging platform is in the inclined-plane tilted to the inside gradually from the bottom to top.
7. detect Reagent adding methods, it is characterized in that, comprise the steps:
Steps A, get a slide, histotomy to be detected is placed on slide;
Step B, the slide carrying histotomy is positioned over one covers with under pilot hole, mobile slide, makes cell sample to be dyed and pilot hole consistency from top to bottom;
Step C, get the upper cover that a top is provided with cylinder, the lower openings of cylinder is positioned at the lower surface of upper cover, the diameter of pilot hole is less than the internal diameter of cylinder, upper cover is fixed on down and covers, make slide between upper cover and lower cover, and ensure that the view field of pilot hole on slide is positioned at the view field of cylinder lower openings on slide; The accurate location of cylinder lower openings view field on slide is positioned at by the projected position determination cell sample of pilot hole on slide;
Step D, mobile coloring agent instillation device, makes the liquid outlet of instillation device be positioned at cylinder upper opening place and is positioned at directly over the above-mentioned position determined, and instillation coloring agent, makes coloring agent drop on slide and cover cell sample.
8. detect Reagent adding methods as claimed in claim 7, it is characterized in that, in step C, the central axis of pilot hole and the central axes of cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310105128.XA CN103217326B (en) | 2013-03-28 | 2013-03-28 | Sample reaction cabin and detection reagent adding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310105128.XA CN103217326B (en) | 2013-03-28 | 2013-03-28 | Sample reaction cabin and detection reagent adding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103217326A CN103217326A (en) | 2013-07-24 |
| CN103217326B true CN103217326B (en) | 2015-05-13 |
Family
ID=48815315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310105128.XA Active CN103217326B (en) | 2013-03-28 | 2013-03-28 | Sample reaction cabin and detection reagent adding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103217326B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468570B (en) * | 2013-09-22 | 2014-09-03 | 江苏省农业科学院 | Glass slide device capable of facilitating single cell gel electrophoresis test |
| CN106769352A (en) * | 2016-11-28 | 2017-05-31 | 广州江元医疗科技有限公司 | A kind of fixture for Cell sheet glass dyeing |
| CN109238812A (en) * | 2018-10-26 | 2019-01-18 | 天津源天晟科技发展有限公司 | A kind of automatic staining device and its control method |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1569653A1 (en) * | 1987-06-12 | 1990-06-07 | Смоленский государственный медицинский институт | Arrangement for fixing biological tissue |
| EP1243911A1 (en) * | 2001-03-22 | 2002-09-25 | Leica Mikrosysteme GmbH | Device for tissue preparation |
| CA2482763C (en) * | 2002-04-26 | 2008-07-29 | Ventana Medical Systems, Inc. | Automated molecular pathology apparatus having fixed slide platforms |
| US7521021B2 (en) * | 2003-11-26 | 2009-04-21 | Leica Biosvstems St. Louis Llc | System for in situ processing of a tissue specimen |
| HUP0800430A2 (en) * | 2008-07-14 | 2010-03-29 | Tibor Dr Glasz | Apparatus and method for fixing of biological tissuesamples |
| CN201359580Y (en) * | 2009-02-24 | 2009-12-09 | 周凤娟 | Staining device for pathological analysis |
| CN201628649U (en) * | 2010-03-15 | 2010-11-10 | 任立明 | Medical inspection staining rack |
| CN201615855U (en) * | 2010-03-19 | 2010-10-27 | 李启生 | Staining jar |
| CN102443540B (en) * | 2010-10-11 | 2013-07-10 | 蒋秀英 | Detachable cell culture chamber |
| CN201903474U (en) * | 2010-12-08 | 2011-07-20 | 福建泰普生物科学有限公司 | Slide dyeing tank structure for dyeing and dyeing machine |
| EP2518468B1 (en) * | 2011-04-26 | 2019-07-24 | Milestone S.r.l. | Method, processor and carrier for processing frozen slices of tissue of biospecimens |
| CN202393768U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Reaction box for immune colloidal gold technology |
| CN102698672B (en) * | 2012-05-21 | 2014-01-29 | 中南大学 | Microfluidic reactor |
| CN202757821U (en) * | 2012-07-11 | 2013-02-27 | 南京金斯瑞生物科技有限公司 | Reserving device for liquid on solid surface |
| CN203191244U (en) * | 2013-03-28 | 2013-09-11 | 广州安必平医药科技有限公司 | Sample reaction bin |
-
2013
- 2013-03-28 CN CN201310105128.XA patent/CN103217326B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103217326A (en) | 2013-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103217326B (en) | Sample reaction cabin and detection reagent adding method | |
| CN205056043U (en) | A disc micro -fluidic chip for in batches quick examination of pesticide residue | |
| CN105784455A (en) | Extraction structure and liquid pretreatment device | |
| CN205080061U (en) | Integration detects cell | |
| CN106563517A (en) | Micro-fluidic chip and detection system for detecting formaldehyde and pH value of textile | |
| CN104931405A (en) | Sealed permeability device and full-automatic concrete anti-permeability instrument adopting the same | |
| CN201687549U (en) | Dry type leakage detecting apparatus of engine oil filter | |
| CN104897547A (en) | Lifting device and full-automatic concrete impermeability instrument adopting same | |
| CN203881499U (en) | Leak detection device for firefighting bottle | |
| CN205607820U (en) | Flexible wall infiltration appearance that measurable body becomes | |
| CN203191244U (en) | Sample reaction bin | |
| CN204705571U (en) | The full-automatic concrete Anti-leakage instrument of sealing permeability apparatus and employing packoff | |
| CN105964314A (en) | Centrifugal type microfluidic chip electrochemical detection device | |
| CN202870092U (en) | Parallel detecting plate of test strip | |
| CN213580824U (en) | Sampling titration detection device for water quality analysis | |
| CN202583178U (en) | Experimental device for measuring soil mineralization potential in large batches | |
| CN208024427U (en) | A kind of drain tooling | |
| CN206710265U (en) | A kind of impervious mechanism for testing of building casting concrete | |
| CN106047661B (en) | PCR detection reagent container and PCR pretreatment system | |
| CN212008236U (en) | Waterproof testing arrangement of cable | |
| CN204694639U (en) | The T-valve measuring apparatus of water quality on-line monitoring instrument | |
| CN204203250U (en) | A kind of sample needle for chemical illumination immunity analysis instrument | |
| CN203793940U (en) | Analysis testing cup used for POCT | |
| CN103175585B (en) | For detecting tester and the method for testing thereof of propping agent blocking solution height | |
| CN206431133U (en) | Portable type full-automatic oil product hydrogen sulfide detector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: Three road 510665 in Guangdong Province, Guangzhou Nanxiang high tech Industrial Development Zone No. 11 self building 7 Applicant after: Guangzhou LBP Medicine Science & Technology Co., Ltd. Address before: 510665, 103, 207, 208 and 214, executive building, 19 Xiangshan Road, Guangzhou, Guangdong, Luogang District Applicant before: Guangzhou Anbiping Medical Science & Technology Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: GUANGZHOU ANBIPING MEDICAL SCIENCE + TECHNOLOGY CO., LTD. TO: GUANGZHOU LBP MEDICINE SCIENCE + TECHNOLOGY CO., LTD. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: 510665 No. 2 Kexin street, Huangpu District, Guangzhou, Guangdong Patentee after: GUANGZHOU LBP MEDICINE SCIENCE AND TECHNOLOGY Co.,Ltd. Address before: 510665 building 7, No. 11, Nanxiang 3rd road, Guangzhou hi tech Industrial Development Zone, Guangdong Province Patentee before: GUANGZHOU LBP MEDICINE SCIENCE AND TECHNOLOGY Co.,Ltd. |
|
| CP02 | Change in the address of a patent holder |