CN103210847A - In-vitro embryo cultivation method for measuring vitality of cynomorium seeds - Google Patents
In-vitro embryo cultivation method for measuring vitality of cynomorium seeds Download PDFInfo
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Abstract
The invention discloses an in-vitro embryo cultivation method for measuring the vitality of cynomorium seeds, and belongs to the field of quality identification and cultivation for noble traditional Chinese medicine seeds in desert regions. The method comprises the following steps of: moving seeds, removing appurtenants from the surfaces of the seeds, breaking hard solids, shelling the seeds, taking cores, cultivating in-vitro embryos and measuring the vitality. By the method, cleanness treatment, appurtenant removal, hard solid breaking and vitality measurement are quickly and effectively performed on the cynomorium seeds; heat injury and mechanical injury as well as death of the cynomorium seeds, which are caused by other treatment, are reduced; the working danger in an operation process is reduced; the working efficiency is high; the repetitiveness is high; and the method is suitable for measuring the vitality of the cynomorium seeds and pre-treating larger-size seeds before planting. The removal rate of the appurtenants on the surfaces of the cynomorium seeds is 94-100 percent; the vitality of the cynomorium seeds is 95-97 percent; and after the appurtenants are removed from the cynomorium seeds, the sprouting time is 10-day ahead.
Description
Technical field
The invention belongs to the expensive traditional Chinese medicine seed quality of desert realm name and identify and cultivation field particularly a kind of stripped embryo culture method that is used for the cynomorium songaricum seed viability test.
Technical background
Cynomorium songaricum (Cynomoriumsongaricum) is that Cynomoriaceae (Cyno-moriaceae) cynomorium songaricum belongs to the parasitic herbaceous seeds plant of (Cynomorium) perennial meat, and multiparasitism is in zygophyllaceae (Zygophyllaceae) Nitraria (Nitraria) plant roots.Similar to the compartment system of white thorn, cynomorium songaricum is born in the salination sand ground of the lake rim in drought desert area and desert district and proluvial, alluvial fan margin zone more, is listed in the top grade of rare traditional Chinese medicine, have the title of " desert genseng ".Modern study shows, cynomorium songaricum antitumor, regulate human immunity, delay senility and prevent and treat aspect such as angiocardiopathy and also have important function.Except that medical value, cynomorium songaricum also can be refined mimosa extract, be used for wine brewing and feed etc., so it has important economy and medical value.Because the predation formula is excavated, the medicine source is constantly reduced in recent years, the cynomorium songaricum wild resource has been in the exhausted state that is on the verge of.For rescue and protection cynomorium songaricum resource, in conjunction with ecological recovery and alkaline land improvement, it is very urgent to carry out the cynomorium songaricum artificial planting at northwest arid area.Kind of skin densification is hard because cynomorium songaricum seed exists, there are complicated hibernation features such as germination inhicbitor matter in the influence of the surface of the seed adjunct, embryo after-ripening, kind intracutaneous, and it is very difficult to cause nature to be sprouted, and has seriously hindered the nature and the artificial propagation of cynomorium songaricum.In recent years, ground such as Inner Mongol, Gansu, Ningxia have launched the artificial infection and the cultivation of cynomorium songaricum in conjunction with ecological construction, but factors such as seed resource is in short supply, quality instability have seriously restricted the development of this industry.At this present situation, many scholars have done big quantity research to the dormancy of how to abolish cynomorium songaricum seed, and expectation is by abolishing the difficulty that its dormancy reduces artificial propagation.Chinese patent CN102742397A discloses a kind of sodium chloride and gibberellin 3 of utilizing and has promoted the method that cynomorium songaricum seed is sprouted, this method can be abolished the dormancy of cynomorium songaricum seed and make it that seed sprouting of 10-37% be arranged in 40 days, this method can be abolished Seed Dormancy to a certain extent, and effectively excites the potential ability of seed sprouting.
The potential ability of seed sprouting is the form of expression of seed vigor, and seed vigor is significant to the germinating capacity and the fast prediction percentage of seedgermination of prediction dormant seed.Because cynomorium songaricum seed costs an arm and a leg, seed is less, and has special hibernation feature, the surface of the seed has adjunct and hard seed rate height, embryo is embedded in the fine and close hard endosperm, with general biochemical method cynomorium songaricum seed is handled, is impelled its germination, measures in its blodynamic process in the prior art, can not abolish Seed Dormancy fully, and occur that seed germination rate is low, seed vigor descends and problem such as repeatable difference.
Summary of the invention
The purpose of this invention is to provide and a kind ofly compare that additive method, simple and easy to do, principle are reliable, the stripped embryo culture method that is used for the cynomorium songaricum seed viability test of favorable repeatability, this stripped embryo culture method can be measured the vitality of seed fast.
Another object of the present invention provides and a kind ofly rejects the seed dermal appendage quickly and safely, abolishes the strong method of cynomorium songaricum seed fast, and this method can reduce manual work and operation danger in the operating process, high efficiency, good reproducibility.
Below content of the present invention and step are done specific description:
The present invention is used for the stripped embryo culture method of cynomorium songaricum seed viability test, and at first skewer is got cynomorium songaricum seed, then described cynomorium songaricum seed is carried out following subsequent processing steps:
A) cleanliness treatment step is described cynomorium songaricum seed is cleaned and to be sieved, and obtains clean seed;
B) adjunct is rejected step, is described clean seed is soaked and stirring by water or alkali, obtains not having the adjunct seed;
C) the strong step of abolishing is randomly drawed the some no adjunct seed that step b) obtains, and carries out the several times repeated experiments, and the aqueous slkali that adopts 1-2mol/L is to described no adjunct seed treatment 3-5h;
D) kind of a benevolence step is got in peeling, is to adopt extrusion to remove kind of a skin, takes out kind of a benevolence, must remove the peel the back and plant benevolence;
E) stripped embryo culture step is that described kind of benevolence is placed on the wetting paper bed of distilled water, cultivates 10-20 days in climate box under 15-25 ℃ of illumination condition, must cultivate the back and plant benevolence;
F) viability test step, be from cultivate the back plant pick out the benevolence hard, present milky or slightly taupe, volume the kind benevolence that increases or have sucker to grow is arranged slightly, as viable seed, and calculate the percentage that the vitality seed is arranged.
Used " the paper bed " of the present invention refers to the special-purpose germination paper of seed, having good water imbibition, moisture capacity, nonhazardous and higher intensity, is the carrier for seed germination and growth of seedling, and preferably the permanent Order in Beijing Science and Technology Ltd. provides, model: HL3-600, specification: 600 * 600mm.After seed sprouting paper is wetting, just be equivalent to the soil of seed growth, a quantity of seeds is sprinkling upon above the seed sprouting paper, seed just can absorb the moisture that is stored in the germination paper, germinates smoothly.When measuring percentage of seedgermination, use seed sprouting paper can make seed be in the wetting state of a standard, and to the uniform absorption of nutrient solution, thereby improved the accuracy that percentage of seedgermination is measured.
" aqueous slkali " of the present invention includes but not limited to alkaline substance solutions such as sodium hydroxide, potassium hydroxide.
" vitality " of the present invention is to represent with " percentage that the vitality seed is arranged ", promptly has the vitality seed to account for for the percentage of examining seed.
The cleanliness treatment step that is used for the stripped embryo culture method of cynomorium songaricum seed viability test of the present invention, be that cynomorium songaricum seed is inserted separator, wind speed with 10.0-15.0m/s is cleaned, again with the sieve of 1.50-1.80mm to seed carry out coarse sizing, the sieve with 0.40-0.80mm carries out fine screening to seed then, obtains clean seed.
First kind of adjunct that is used for the stripped embryo culture method of cynomorium songaricum seed viability test of the present invention rejected step, be described clean seed to be carried out 80-100 ℃ hot water scald kind, be cooled to room temperature and guarantee water logging kind 24h, crumple the surface of the seed with nylon gauze then.
Second kind of stripped embryo culture method that is used for the cynomorium songaricum seed viability test of the present invention, described adjunct is rejected step, is described clean seed is under agitation handled 3min with 1% NaOH solution soaking.
Adopt the NaOH solution-treated of boiling hot kind of hot water and soak at room temperature and 1% simple to the appendicular rejecting of seed, processing is clean, and the treatment effeciency height does not damage seed.
The stripped embryo culture method that is used for the cynomorium songaricum seed viability test of the present invention, the described strong step of abolishing is randomly drawed the some no adjunct seed that step c) obtains, and carries out the several times repeated experiments, adopts the NaOH solution of 1mol/L that cynomorium songaricum seed is handled 5h.
The stripped embryo culture method that is used for the cynomorium songaricum seed viability test of the present invention, the described strong step of abolishing, randomly draw the some no adjunct seed that step c) obtains, carry out the several times repeated experiments, adopt the NaOH solution of 1.5mol/L that cynomorium songaricum seed is handled 4h.
It is strong to adopt alkaline solution such as NaOH to abolish seed, and method is simple, and is with low cost, environmentally safe, to kind of a benevolence not damaged, and abolish strong after, plant the skin deliquescing, plant benevolence to be easy to take out, help data statistics.
The stripped embryo culture method that is used for the cynomorium songaricum seed viability test of the present invention, described stripped embryo culture step: be that peeling is placed on the wetting paper bed of distilled water, in climate box, cultivated 15 days under 20 ℃ of illumination conditions.
First kind of stripped embryo culture method that preferably is used for the cynomorium songaricum seed viability test of the present invention comprises the steps:
A) skewer kind step: skewer is got the cynomorium songaricum seed sample;
B) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected step: will described clean seed with 1% NaOH solution soaking handle 3min, and continuous stirring obtain not having the adjunct seed;
D) the strong step of abolishing: randomly drawing the some no adjunct seed that step c) obtains, carry out the several times repeated experiments, is to adopt the NaOH solution of 1.5mol/L to described no adjunct seed treatment 4h;
E) the benevolence step is got in peeling: be to adopt extrusion to remove kind of a skin, take out kind of a benevolence, must remove the peel the back and plant benevolence;
F) stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 15 days in climate box under 20 ℃ of illumination conditions;
G) viability test step, from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume and have slightly to increase or plant and seed that sucker grows is arranged in the benevolence as viable seed, and calculating has the percentage of vitality seed.
Second kind of stripped embryo culture method that preferably is used for the cynomorium songaricum seed viability test of the present invention comprises the steps:
A) skewer kind step: skewer is got the cynomorium songaricum seed sample;
B) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected step: described clean seed is carried out boiling hot kind of hot water, be cooled to room temperature and guarantee water logging kind 24h, crumple the surface of the seed with nylon gauze then;
D) the strong step of abolishing: randomly draw the some no adjunct seed that step c) obtains, carry out the several times repeated experiments, the NaOH solution that adopts 2mol/L is to described no adjunct seed treatment 3h;
E) the benevolence step is got in peeling: be to adopt extrusion to remove kind of a skin, take out kind of a benevolence, obtain removing the peel the back and plant benevolence;
F) stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 15 days in climate box under 20 ℃ of illumination conditions;
G) viability test step, from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume and have slightly to increase or plant and seed that sucker grows is arranged in the benevolence as viable seed, and calculating has the percentage of vitality seed.
The present invention also provides a kind of and has rejected the seed dermal appendage quickly and safely, abolished the method that cynomorium songaricum seed is strong, obtain complete kind of benevolence fast, and described method comprises the steps:
A) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
B) adjunct is rejected step: described clean seed is carried out boiling hot kind of hot water, be cooled to room temperature and guarantee water logging kind 24h, crumple the surface of the seed with nylon gauze then;
C) the strong step of abolishing: be to adopt the NaOH solution of 2mol/L to described no adjunct seed treatment 3h;
D) the benevolence step is got in peeling: the employing extrusion is removed kind of a skin, takes out kind of a benevolence, gets complete kind of benevolence.
The beneficial effect that is used for the stripped embryo culture method of cynomorium songaricum seed viability test of the present invention is: provide the stripped embryo culture method of a kind of usefulness that the vitality of cynomorium songaricum seed is carried out method for measuring, the embryo culture method that should exsomatize is measured technology, be to abolish strong with physics and chemical method removal kind of a skin adjunct, chemical method, make kind of benevolence and embryo under the not damaged state, take out a kind of technology of cultured in vitro in climate box fast.This method can directly be judged seed vigor according to kind of benevolence state, compares additive method, and simple and easy to do, principle is reliable, no machinery and chemical damage, favorable repeatability; The appendicular rejecting rate in cynomorium songaricum seed surface is 94%-100%, and the vitality of cynomorium songaricum seed is 95%-97%; Cynomorium songaricum seed is after rejecting adjunct, and sprouting time shifts to an earlier date ten days.
In addition, of the present inventionly a kind ofly reject the seed dermal appendage quickly and safely, abolish the strong method of cynomorium songaricum seed fast, can reduce manual work and operation danger in the operating process, high efficiency, good reproducibility.
Embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1
First kind of stripped embryo culture method that is used for the cynomorium songaricum seed viability test comprises the steps:
A) skewer is got wild abundant ripe cynomorium songaricum seed 500g;
B) cynomorium songaricum seed is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected: stir down and handle 3min with 1% NaOH solution soaking, obtain not having the adjunct seed, the rejecting rate reaches 100%;
D) strong abolishing: randomly draw 400 in the no adjunct seed that step c) obtains, carry out 4 times each 100 repeated experiments, cynomorium songaricum seed is handled 5h with the NaOH solution of 1mol/L;
E) remove kind of a skin, take out kind of a benevolence: with tweezers extruding peeling, take out kind of a benevolence, must remove the peel the back and plant benevolence;
F) stripped embryo culture: will remove the peel back kind benevolence and place on the wetting paper bed of distilled water, and under 0 ℃ of illumination condition, in climate box, cultivate 15 days;
G) viability test: from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume have slightly increase or kind benevolence in seed that sucker grows is arranged as viable seed, and will plant benevolence brown, oedema, the seed that goes mouldy removes as no vitality seed, and calculates the percentage that the vitality seed is arranged.
Measurement result: the vitality of cynomorium songaricum seed is 97%.
Embodiment 2
Second kind of stripped embryo culture method that is used for the cynomorium songaricum seed viability test comprises the steps:
A) skewer is got wild abundant ripe cynomorium songaricum seed 500g;
B) cleanliness is handled: cynomorium songaricum seed is inserted separator, cleans with the wind speed of 10m/s, again with 1.5 sieve to seed carry out coarse sizing, the sieve with 0.40mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected: scald with 90 ℃ hot water and plant, be cooled to room temperature and guarantee water logging kind 24h, then with nylon gauze rubbing, rejecting rate 96.7%;
D) strong abolishing: randomly draw 400 in the no adjunct seed that step c) obtains, carry out 4 times each 100 repeated experiments, cynomorium songaricum seed is handled 4h with the NaOH solution of 1.5mol/L;
E) remove kind of a skin, take out kind of a benevolence: with tweezers extruding peeling, take out kind of a benevolence, must remove the peel the back and plant benevolence;
F) stripped embryo culture: will remove the peel back kind benevolence and place on the wetting paper bed of distilled water, and in climate box, cultivate 20 days under 15 ℃ of illumination conditions;
G) viability test: from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume have slightly increase or kind benevolence in seed that sucker grows is arranged as viable seed, and will plant benevolence brown, oedema, the seed that goes mouldy removes as no vitality seed, and calculates the percentage that the vitality seed is arranged.
Measurement result: the vitality of cynomorium songaricum seed is 96%.
Embodiment 3
The stripped embryo culture method that the third is used for the cynomorium songaricum seed viability test comprises the steps:
A) skewer is got wild abundant ripe cynomorium songaricum seed 500g;
B) cleanliness is handled: cynomorium songaricum seed is inserted separator, cleans with the wind speed of 15m/s, again with 1.8 sieve to seed carry out coarse sizing, the sieve with 0.8mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected: scald with 100 ℃ hot water and plant, be cooled to room temperature and guarantee water logging kind 24h, then with nylon gauze rubbing, rejecting rate 94%;
D) strong abolishing: randomly draw 400 in the no adjunct seed that step c) obtains, carry out 4 times each 100 repeated experiments, cynomorium songaricum seed is handled 3h with the NaOH solution of 2.0mol/L;
E) remove kind of a skin, take out kind of a benevolence: with tweezers extruding peeling, take out kind of a benevolence, must remove the peel the back and plant benevolence;
F) stripped embryo culture: will remove the peel back kind benevolence and place on the wetting paper bed of distilled water, and in climate box, cultivate 10 days under 25 ℃ of illumination conditions;
G) viability test: from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume have slightly increase or kind benevolence in seed that sucker grows is arranged as viable seed, and will plant benevolence brown, oedema, the seed that goes mouldy removes as no vitality seed, and calculates the percentage that the vitality seed is arranged.
Measurement result: the vitality of cynomorium songaricum seed is 95%.
Claims (10)
1. a stripped embryo culture method that is used for the cynomorium songaricum seed viability test is at first carried out skewer and is got the cynomorium songaricum seed step, it is characterized in that, described stripped embryo culture method also comprises the following subsequent processing steps of described cynomorium songaricum seed:
A) cleanliness treatment step is described cynomorium songaricum seed is cleaned and to be sieved, and obtains clean seed;
B) adjunct is rejected step, is described clean seed is passed through water or aqueous slkali soaking, obtains not having the adjunct seed;
C) the strong step of abolishing is randomly drawed the some no adjunct seed that step b) obtains, and carries out the several times repeated experiments, and the aqueous slkali that adopts 1-2mol/L is to described no adjunct seed treatment 3-5h;
D) the benevolence step is got in peeling, is to adopt extrusion to remove kind of a skin, must remove the peel the back and plant benevolence;
E) stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 10-20 days in climate box under 15-25 ℃ of illumination condition, must cultivate back kind benevolence;
F) viability test step, be from cultivate the back plant pick out the benevolence hard, present milky or slightly taupe, volume the kind benevolence that increases or have sucker to grow is arranged slightly, as viable seed, and calculate the percentage that the vitality seed is arranged.
2. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1, it is characterized in that, described cleanliness treatment step, be that described cynomorium songaricum seed is inserted separator, wind speed with 10.0-15.0m/s is cleaned, sieve with 1.50-1.80mm carries out coarse sizing again, the sieve with 0.40-0.80mm carries out fine screening then, obtains clean seed.
3. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1, it is characterized in that, described adjunct is rejected step, be described clean seed to be carried out 80-100 ℃ hot water scald kind, be cooled to room temperature, and continue seed soaking 24h, crumple the surface of the seed with nylon gauze then.
4. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1, it is characterized in that, described aqueous slkali is 1% NaOH solution, and it is that described clean seed is under agitation handled 3min with 1% NaOH solution soaking that described adjunct is rejected step.
5. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1 is characterized in that described aqueous slkali is the NaOH solution of 1mol/L, and the described strong step of abolishing is to adopt the NaOH solution of 1mol/L that cynomorium songaricum seed is handled 5h.
6. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1, it is characterized in that, described aqueous slkali is the NaOH solution of 1.5mol/L, and the described strong step of abolishing is to adopt the NaOH solution of 1.5mol/L that cynomorium songaricum seed is handled 4h.
7. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1, it is characterized in that, described stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 15 days in climate box under 20 ℃ of illumination conditions.
8. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1 is characterized in that, comprises the steps:
A) skewer kind step: skewer is got cynomorium songaricum seed;
B) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected step: will described clean seed with 1% NaOH solution soaking handle 3min, and continuous stirring obtain not having the adjunct seed;
D) the strong step of abolishing: randomly draw the some no adjunct seed that step c) obtains, carry out the several times repeated experiments, the NaOH solution that adopts 1.5mol/L is to described no adjunct seed treatment 4h;
E) the benevolence step is got in peeling: be to adopt extrusion to remove kind of a skin, must remove the peel the back and plant benevolence;
F) stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 15 days in climate box under 20 ℃ of illumination conditions;
G) viability test step, be from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume and have slightly to increase or plant and seed that sucker grows is arranged in the benevolence as viable seed, and calculating has the percentage of vitality seed.
9. the stripped embryo culture method that is used for the cynomorium songaricum seed viability test according to claim 1 is characterized in that, comprises the steps:
A) skewer kind step: skewer is got cynomorium songaricum seed;
B) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
C) adjunct is rejected step: described clean seed is carried out boiling hot kind of 100 ℃ of hot water, be cooled to room temperature and guarantee water logging kind 24h, crumple the surface of the seed with nylon gauze then;
D) the strong step of abolishing: randomly draw the some no adjunct seed that step c) obtains, carry out the several times repeated experiments, the NaOH solution that adopts 2mol/L is to described no adjunct seed treatment 3h;
E) the benevolence step is got in peeling: be to adopt extrusion to remove kind of a skin, take out kind of a benevolence, obtain removing the peel the back and plant benevolence;
F) stripped embryo culture step is benevolence to be planted in described peeling back place on the wetting paper bed of distilled water, cultivates 15 days in climate box under 20 ℃ of illumination conditions;
G) viability test step, be from cultivate the back plant pick out the benevolence through cultivating after, plant benevolence hard, present milky or slightly taupe kind benevolence, plant the benevolence volume and have slightly to increase or plant and seed that sucker grows is arranged in the benevolence as viable seed, and calculating has the percentage of vitality seed.
10. reject the seed dermal appendage quickly and safely, abolish the strong method of cynomorium songaricum seed fast for one kind, it is characterized in that described method comprises the steps:
A) cleanliness treatment step: the described cynomorium songaricum seed that skewer is got is inserted separator, cleans with the wind speed of 12.6m/s, again with the sieve of 1.62mm to seed carry out coarse sizing, the sieve with 0.60mm carries out fine screening to seed then, obtains clean seed;
B) adjunct is rejected step: described clean seed is carried out boiling hot kind of 100 ℃ of hot water, be cooled to room temperature and guarantee water logging kind 24h, crumple the surface of the seed with nylon gauze then;
C) the strong step of abolishing: randomly draw the some no adjunct seed that step b) obtains, carry out the several times repeated experiments, the NaOH solution that adopts 2mol/L is to described no adjunct seed treatment 3h;
D) the benevolence step is got in peeling: the employing extrusion is removed kind of a skin, takes out kind of a benevolence, gets complete kind of benevolence.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109662029A (en) * | 2018-12-20 | 2019-04-23 | 河西学院 | A kind of band endotesta isolated seed embryo method of shiny-leaved yellowhorn seed vigor measurement |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109662029A (en) * | 2018-12-20 | 2019-04-23 | 河西学院 | A kind of band endotesta isolated seed embryo method of shiny-leaved yellowhorn seed vigor measurement |
| CN109662029B (en) * | 2018-12-20 | 2021-08-03 | 河西学院 | Method for culturing in-band seed coat in-vitro embryo for determining viability of xanthoceras sorbifolia seeds |
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