CN103168609B - Pleione seed original habitat natural sowing method - Google Patents

Pleione seed original habitat natural sowing method Download PDF

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Publication number
CN103168609B
CN103168609B CN201310135669.7A CN201310135669A CN103168609B CN 103168609 B CN103168609 B CN 103168609B CN 201310135669 A CN201310135669 A CN 201310135669A CN 103168609 B CN103168609 B CN 103168609B
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seed
matrix
pleione bulbocodioides
pleione
capsule
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CN103168609A (en
Inventor
朱国胜
张丽娜
桂阳
王沁
张金霞
陈娅娅
杨通静
卢颖颖
金吉芬
蓝家望
龚光禄
李青风
冉孝琴
黄万兵
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GUIZHOU CROPS VARIETIES RESOURCE INSTITUTE
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GUIZHOU CROPS VARIETIES RESOURCE INSTITUTE
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Abstract

The invention discloses a pleione seed original habitat natural sowing method which comprises the following steps of: (1) performing artificial pollination on pleione; (2) harvesting and selecting capsules; (3) storing the capsule; (4) treating seeds before sowing; (5) selecting a sowing place; (6) arranging a sample land; and (7) sowing the seeds. According to the method, the advantage of a large number of pleione capsule seeds is fully used; due to a mild environment condition of an original habitat of the pleione, symbiotic germination and growth of the complicated pleione seeds are simplified; a pleione seed original habitat germination technical method is constructed; the production cost of seedlings is greatly saved; furthermore, the seedlings is high in adaptability to the environment of the original habitat and is easy to transplant and survive; and the method is a technology deserving to popularize.

Description

Pleione bulbocodioides seed Proterozoic self seed method
Technical field
The present invention relates to field of agricultural sciences, especially a kind of pleione bulbocodioides seed Proterozoic self seed method.
Background technology
Along with the medical value of pleione bulbocodioides and continually developing of ornamental value, wild pleione bulbocodioides resource is excavated without restraint, and resource is endangered, increasingly outstanding to the importance of pleione bulbocodioides protection of resources and sustainable use.At present, pleione bulbocodioides cultivation is mature on the whole, but planting source problem does not solve; wild pleione bulbocodioides kind ball main or that utilization is excavated carries out domesticating and cultivating; reproduction coefficient is very low, can not meet protection and the sustainable use of resource, causes on the contrary wild resource to be destroyed by more massive excavation.The same with other orchids, in pleione bulbocodioides capsule, contain tens thousand of seeds, utilize seed to breed, be the only way that solves pleione bulbocodioides kind source problem.The development of modern tissue culture technology, has successfully realized pleione bulbocodioides seed germination acquisition group training seedling, but group training seedling white silk transplantation of seedlings technology is immature, and transplanting survival rate is low, causes group training seedling cost very high.
Summary of the invention
The object of the invention is: a kind of pleione bulbocodioides seed Proterozoic self seed method is provided, and its material source is extensive, simple to operate, cost is low, solved the problem of pleione bulbocodioides sapling multiplication difficulty, realizes pleione bulbocodioides protection of resources and sustainable use.
The present invention is achieved in that pleione bulbocodioides seed Proterozoic self seed method, comprises the following steps:
(1) pleione bulbocodioides artificial pollination: pleione bulbocodioides carries out pleione bulbocodioides artificial pollination after blooming March~April;
(2) the gathering and selecting of capsule: the capsule of timely collecting pleione bulbocodioides, select full capsule;
(3) storage of capsule: the capsule of choosing is installed by ox-hide paper envelopes, be positioned in discolour silica gel and be dried, then place refrigerator cold-storage;
(4) seed treatment before sowing: seed is shaked out from capsule, admix matrix;
(5) selection on sowing ground: selections height above sea level is 1200m ~ 1400m, has wild pleione bulbocodioides distribution, what vegetation was suitable face south smooth sandy loam;
(6) arrangement on sample ground: cut off vegetation, dig out the above plant roots of diameter 0.5cm, retain thinner root system of plant, the surface 4~6cm detritus layer soil of turning over is concentrated for subsequent use, then the detritus layer of the 4~6cm of lower floor that turns over, is organized into ridge-up bed for subsequent use;
(7) sowing: will be sown into ridge-up bed without seed matrix, and then seed dressing matrix be broadcasted sowing in this matrix, and broadcast one deck without seed matrix on it again, top layer lid is concentrated detritus soil 4~6cm for subsequent use, then covers the cogongrass moisturizing of one deck 3~5cm.
Pleione bulbocodioides artificial pollination described in step (1), concrete pleione bulbocodioides pollination time is to open in 1~3 day at the flower of pleione bulbocodioides, utilizes toothpick picking flower pesticide, and openend is sticked to column cap.The general flowering time of pleione bulbocodioides is March~April, florescence is approximately about 1 month, its pollination time can be very long accordingly, but practical work experience shows, pleione bulbocodioides flower bag opens in 1~3, the viscosity of flower pesticide and column cap is large, easily pollinate successfully, along with the prolongation of time, flower pesticide and column cap become dry, flower pesticide is difficult to adhere on column cap, causes pollination failures.
The collecting time of the pleione bulbocodioides capsule described in step (2) is latter 190 days of pollination, and the selection criteria of ripe high-quality capsule is apparent full, and hand is pinched flexible.After inventor's research shows the pollination of pleione bulbocodioides capsule, before 190 days, collect seed, seed is easily sprouted under Proterozoic natural conditions, but in its further growth course, lethality is very high.If the time exceedes 190 days after correlative study also shows to pollinate, although seed is ripe, germination rate reduces greatly.And the rear seed germination rate of adopting for about 190 days of pollinating is high, and lethality in further growth growth course is low.Watch quietly fullly, hand pinches that resilient seed tool embryo rate is high, and not full or hand is pinched the soft general depauperation of capsule seed, and seed tool embryo rate is low, is not suitable for planting.
In step (3), the capsule silica dehydrator time is 1 week, and refrigerated storage temperature is 4 DEG C, and cold preservation time is 2~5 months.The capsule of gathering is preferably now broadcast, and still, pleione bulbocodioides seed is ripe about October, if broadcast, can cause seed to be sprouted very soon, but next weather turns cold at once, and the seed of sprouting can be freezed to death.Adopt the seed getting off and need store sowing in 3~April of Second Year for well, now, the rising of weather temperature, seed can be sprouted smoothly, can also further growth develop into seedling.Pleione bulbocodioides is the same with other Orchid Seeds, after preservation, can enter resting state, and through low temperature, processing can break through dormancy to the seed demand of resting state, and Second Year could be sprouted.So the pleione bulbocodioides capsule of gathering is needed to low-temperature preservation, in preserving process the control of humidity very important, the too high meeting of humidity cause capsule go rotten, our research shows, utilizes silica gel drying at room temperature to preserve after 1 week again, capsule can not go rotten.
Step (4) mesostroma refers to the matrix of preparing after broad-leaved tree sawmilling, broad-leaved tree leaf powder mince or waste edible fungus bacterium bag becomes thoroughly decomposed, or liver moss pulverize after matrix, or the matrix that various matrix arbitrary proportions are hybridly prepared into above, the humidity of matrix is controlled at 60~70%; Seed is admixed the method for matrix for matrix is tiled in north feed basin, is stained with seed is sprinkled into seed to stromal surface shake with writing brush, then matrix is mixed even.Broad-leaved tree sawmilling, the broad-leaved tree leaf powder that utilization is become thoroughly decomposed mince waste edible fungus bacterium bag or pulverize after liver moss be mainly as matrix, three kinds of matrix are not become thoroughly decomposed above, Germination Strain in Proterozoic soil is just difficult to utilize these matrix growths, just can not be well and Seed Development symbiotic relation, seed just can not normally be sprouted and grow; In nature, pleione bulbocodioides is normal together with liver moss symbiosis, correlative study report also shows that liver moss, fungal component and pleione bulbocodioides form Trinitarian symbiotic relation, pleione bulbocodioides can obtain nutriment from liver moss by symbiosis fungi, realize and sprout and grow smoothly, this explanation symbiosis fungi can be in the growth well on liver moss of living, so liver moss does not need to become thoroughly decomposed.
Selecting height above sea level in step (5) is 1200m ~ 1400m, has wild pleione bulbocodioides and distributes, what vegetation was suitable face south smooth sandy loam.Suitable vegetation is mainly sparse undershrub or cogongrass clump.Show through inventor's investigation, wild pleione bulbocodioides is mainly distributed in 1200~1400 meters of height above sea level, and in the undershrub that tailo is sparse or cogongrass clump, soil is loose sandy loam.The wild pleione bulbocodioides of dense undershrub or cogongrass clump distributes few, in the dense woods, distribute without pleione bulbocodioides, this shows that sparse undershrub or cogongrass clump have certain light transmittance, temperature and humidity is suitable, air permeability is also better simultaneously, the fungal component growth and the pleione bulbocodioides that are suitable in soil grow, so select this environment sowing.
Step is cut off vegetation in (6), digs out the above plant roots of diameter 0.5cm, retains thinner root system of plant, and the surface 4~6cm detritus layer soil of turning over is concentrated for subsequent use, then the detritus layer of the 4~6cm of lower floor that turns over, is organized into wide 1m ridge-up bed.To remove so large root is unfavorable for sowing, retain rootlet and be because these rootlets can crop soil in the nutrient source of symbiosis fungi.The top layer detritus soil of surface 4~6cm turned over is concentrated for subsequent use, is because wherein nutritious, and symbiosis fungal species is also abundant, and under nature, pleione bulbocodioides is also grown in this one deck, so this layer of soil is most important, can concentrate the overburden soil as after planting.The turn over detritus soil of the 4~6cm of lower floor, is to increase ventilation, and the growth to fungal component and the germination and growth of seed are favourable.The wide 1m of ridge-up bed is convenient to stand in the both sides operation of furrow, too wide has operated with regard to bad.
The described sowing of step (7) specifically refers to, it is wide 3~5 centimetres that lower floor is sown into ridge-up bed without seed matrix, thick 0.3~0.5cm; The seed dressing matrix that broadcast sowing in middle level is wide 3~5 centimetres, thick 0.1~0.2cm, and upper strata is wide 3~5 centimetres without seed matrix, thick 0.3~0.5cm, top layer lid is concentrated detritus soil 4~6cm for subsequent use, sowing media strip length 80cm, then cover the cogongrass moisturizing of one deck 3~5cm.The two-layer effect mainly as nutrient source and moisturizing without seed matrix, fungal component in soil can grow to matrix, along with the growth of fungal component, can progressively set up matrix, symbiosis fungi and the Trinitarian symbiotic relation of pleione bulbocodioides, realize the lasting supply of pleione bulbocodioides nutrition, thereby reach the object of sprouting and growing.Upper strata covers cogongrass can regulate temperature on the one hand, prevents direct sunlight, on the other hand the humidity that keeps soil is had to important effect, and temperature and humidity is also the key factor of the smooth sprouting of pleione bulbocodioides seed and growth.
Owing to having adopted above-mentioned technical scheme, the present invention has utilized the large advantage of pleione bulbocodioides capsule seed amount fully, combine pleione bulbocodioides Proterozoic control environment condition, complicated pleione bulbocodioides seed symbiotic germination and the problem of growing are simplified, set up pleione bulbocodioides seed Proterozoic Germination Technique method, greatly saved seedling production cost, and the seedling of producing has been strong to the suitability of Proterozoic environment, being easy to transplant survival, is the technology being worthy to be popularized.The present invention is directed to these key issues in production and research process, set up the method for pleione bulbocodioides seed Proterozoic self seed, realized the Proterozoic of pleione bulbocodioides seedling and sprouted, for the large-scale production of pleione bulbocodioides seedling Proterozoic is laid a good foundation.
Embodiment
Embodiments of the invention: pleione bulbocodioides seed Proterozoic self seed method, comprise the following steps: (1) pleione bulbocodioides artificial pollination: pleione bulbocodioides is bloomed March~April, flower bag forms and blooms and starts in 1~3 day, utilize toothpick picking flower pesticide, openend is sticked to column cap, complete pleione bulbocodioides artificial pollination; (2) the gathering and selecting of capsule: pleione bulbocodioides is pollinated latter 190 days, and capsule maturation, can gather, selects apparent fullly, and hand is pinched flexible for subsequent use; (3) storage of capsule: capsule installs by ox-hide paper envelopes, drying at room temperature 1 week in placement and discolour silica gel, the refrigerator and cooled that is then placed on 4 DEG C is hidden 2~5 months; (4) seed treatment before sowing: seed is shaked out from capsule, admix the matrix of preparation after broad-leaved tree sawmilling, broad-leaved tree leaf powder are minced or waste edible fungus bacterium bag becomes thoroughly decomposed, or liver moss pulverize after matrix, or the matrix that is hybridly prepared into of various matrix arbitrary proportions, the humidity of matrix is controlled at 60~70%; Seed is admixed the method for matrix for matrix being paved in north feed basin to skim, is stained with seed is sprinkled into seed to stromal surface shake with writing brush, then matrix is mixed even; (5) selection on sowing ground: selection height above sea level is 1200m ~ 1400m, has wild pleione bulbocodioides and distributes, the sparse undershrub of the smooth sandy loam that faces south or cogongrass clump; (6) arrangement on sample ground: cut off vegetation, dig out the above plant roots of diameter 0.5cm, retain thinner root system of plant, surface 4~6cm detritus layer the soil of turning over is concentrated for subsequent use, then the detritus layer of the 4~6cm of lower floor that turns over, is organized into wide 1m ridge-up bed for subsequent use, length is not limit, can be fixed according to place; (7) sowing: it is wide 3~5 centimetres that lower floor is sown into ridge-up bed without seed matrix, thick 0.3~0.5cm; The seed dressing matrix that broadcast sowing in middle level is wide 3~5 centimetres, thick 0.1~0.2cm, upper strata is wide 3~5 centimetres without seed matrix, thick 0.3~0.5cm, sowing media strip length 80cm, distance between sowing media band is 5~10cm, and top layer lid is concentrated detritus soil 4~6cm for subsequent use, then covers the cogongrass moisturizing of one deck 3~5cm.

Claims (8)

1. a pleione bulbocodioides seed Proterozoic self seed method, is characterized in that: comprise the following steps:
(1) pleione bulbocodioides artificial pollination: pleione bulbocodioides, after blooming March~April, opens and carries out pleione bulbocodioides artificial pollination in 1~3 day at the flower of pleione bulbocodioides;
(2) the gathering and selecting of capsule: the collecting time of pleione bulbocodioides capsule is latter 190 days of pollination, and the capsule of the pleione bulbocodioides of gathering, selects full capsule;
(3) storage of capsule: the capsule of choosing is installed by ox-hide paper envelopes, be positioned over drying at room temperature in discolour silica gel, then place refrigerator cold-storage;
(4) seed treatment before sowing: seed is shaked out from capsule, admix matrix;
(5) selection on sowing ground: selections height above sea level is 1200m ~ 1400m, has wild pleione bulbocodioides distribution, what vegetation was suitable face south smooth sandy loam;
(6) arrangement on sample ground: cut off vegetation, dig out plant roots more than diameter 0.5cm, the surface 4~6cm detritus layer soil of turning over is concentrated for subsequent use, then the detritus layer of the 4~6cm of lower floor that turns over, is organized into ridge-up bed for subsequent use;
(7) sowing: will be sown into ridge-up bed without seed matrix, and then seed dressing matrix be broadcasted sowing in this matrix, and broadcast one deck without seed matrix on it again, top layer lid is concentrated detritus soil 4~6cm for subsequent use, then covers the cogongrass moisturizing of one deck 3~5cm.
2. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: the pleione bulbocodioides artificial pollination described in step (1) is to utilize toothpick picking flower pesticide, and openend is sticked to column cap.
3. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: in step (2), the selection criteria of ripe high-quality capsule is apparent full, and hand is pinched flexible.
4. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: in step (3), the capsule silica dehydrator time is 1 week, and refrigerated storage temperature is 4 DEG C, and cold preservation time is 2~5 months.
5. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, it is characterized in that: step (4) mesostroma refers to the matrix of preparing after broad-leaved tree sawmilling, broad-leaved tree leaf powder mince or waste edible fungus bacterium bag becomes thoroughly decomposed, or liver moss pulverize after matrix, or the matrix that various matrix arbitrary proportions are hybridly prepared into above, the humidity of matrix is controlled at 60~70%; Seed is admixed the method for matrix for matrix is tiled in plastic basin, is stained with seed is sprinkled into seed to stromal surface shake with writing brush, then matrix is mixed even.
6. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: selecting height above sea level in step (5) is 1200m ~ 1400m, has wild pleione bulbocodioides and distributes, what vegetation was suitable face south smooth sandy loam.
7. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: in step (6), the ridge-up bed of arrangement is wide is 1m.
8. pleione bulbocodioides seed Proterozoic self seed method according to claim 1, is characterized in that: the described sowing of step (7) specifically refers to, lower floor is sown into the wide 3~5 centimetres of ridge-up bed, thick 0.3~0.5cm without seed matrix; The seed dressing matrix that broadcast sowing in middle level is wide 3~5 centimetres, thick 0.1~0.2cm, and upper strata is wide 3~5 centimetres without seed matrix, thick 0.3~0.5cm, top layer lid is concentrated detritus soil 4~6cm for subsequent use, sowing media strip length 80cm, then cover the cogongrass moisturizing of one deck 3~5cm.
CN201310135669.7A 2013-04-18 2013-04-18 Pleione seed original habitat natural sowing method Expired - Fee Related CN103168609B (en)

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CN103444394B (en) * 2013-08-15 2015-02-25 李子辉 Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition
CN104770281B (en) * 2015-04-16 2016-11-30 四川农业大学 A kind of pleione bulbocodioides florescence control method
CN104770194B (en) * 2015-04-27 2017-01-25 四川农业大学 Pleione pseudobulb cultivating method
CN106258992B (en) * 2016-10-19 2018-07-17 贵州省农作物品种资源研究所 Pleione bulbocodioides tissue-cultured seedling Proterozoic hardening off method
CN106332782B (en) * 2016-10-19 2018-10-19 贵州省农作物品种资源研究所 Pleione bulbocodioides tissue cultures one-step culture method
CN106941890A (en) * 2017-03-23 2017-07-14 杨军 A kind of storage method of willow seed
CN107211636A (en) * 2017-06-12 2017-09-29 胡柳新 A kind of type of seeding of plant
CN109618861A (en) * 2018-12-25 2019-04-16 贵州省农作物品种资源研究所 A kind of method for planting of pleionebulbocodioides rolfe

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