CN103122313A - Superfine crushing wall-breaking method of haematococcus pluvialis cell - Google Patents
Superfine crushing wall-breaking method of haematococcus pluvialis cell Download PDFInfo
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- CN103122313A CN103122313A CN2013100829462A CN201310082946A CN103122313A CN 103122313 A CN103122313 A CN 103122313A CN 2013100829462 A CN2013100829462 A CN 2013100829462A CN 201310082946 A CN201310082946 A CN 201310082946A CN 103122313 A CN103122313 A CN 103122313A
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Abstract
The invention relates to a wall-breaking method of algae, and in particular relates to a superfine crushing wall-breaking method of a haematococcus pluvialis cell. According to the method, superfine crushing equipment is adopted and is matched with liquid nitrogen equipment, so that the haematococcus pluvialis is frozen instantaneously to become brittle. Therefore, the wall-breaking rate is improved, and the protection environment of a low temperature and oxygen isolation are realized. After the wall-breaking treatment is carried out on the haematococcus pluvialis cell by using the method, the active substance of the haematococcus pluvialis cell has a high leaching rate and is not oxidated, so that the extraction ratio of the active substance is improved. The method is simple in process and convenient to operate, and can be used for performing the one-step treatment on a small quantity of samples in a short time and can also be matched with continuous equipment, sample collection equipment and the like to realize the automatic and continuous production, i.e., the mechanization degree is higher, so that the method is suitable for industrialized production. In addition, the samples are not polluted during the equipment running process, so that the safety is high.
Description
Technical field
The present invention relates to the wall-breaking method of a kind of algae, particularly a kind of micronizing wall-breaking method of haematococcus pluvialis cell.
Background technology
Haematocoocus Pluvialls (
haematoccoccuspluvialis) be a kind of widely distributed unicell green alga, can form chlamydospore the multiple carotenoid with physiological function of a large amount of accumulation under adverse environmental factor: astaxanthin, alpha-carotene, β-carotene, xenthophylls, cryptoxanthin, zeaxanthin, canthaxanthin etc., wherein 80% is astaxanthin and ester class thereof, Haematocoocus Pluvialls is the highest Biological resources of current known natural astaxanthin content, in October, 2010, Ministry of Health's approval Haematocoocus Pluvialls is new resource food, utilize Haematococcus pluvialis production carotene active component to have broad prospects, but spore of haematococcus pluvialis cell wall thickness and very hard, be difficult for broken, can stop passing through of the compositions such as astaxanthin, can only adopt some technology that cell is carried out to broken wall treatment, while carotene constituents, particularly astaxanthin is in illumination, very easily oxidation stain under high temperature or oxygen atmosphere, thereby need to select suitable wall breaking technology.
The more Haematocoocus Pluvialls wall breaking technology of research mainly contains homogenate method, polishing, enzyme process, supersonic method etc. at present; these methods are all less is applied to large-scale industrial production; thereby for developing better the Haematocoocus Pluvialls resource; particularly realize the large-scale industrial production of endocellular function composition; need a kind of simple and effective, the wall breaking technology that mechanization degree is high.Superfine communication technique is a kind of various solid matters to be ground into to the micron technology of nanometer grade powder even, main by reactive forces such as centrifugal force, mechanical shear stress and air-flow surging forces, the effects such as high-pressure homogeneous, hole, even instantaneous in short-term, material is carried out to fragmentation, and can under the environment of low temperature, drying, sealing, realize, avoided loss and the structural changes of activeconstituents in the material.Thereby this technology has the characteristics such as quick, efficient, easy and simple to handle, continuously-running, in the industries such as food, medicine and chemical industry, all be widely used, be applied at present suitability for industrialized production.
But adopt the Haematocoocus Pluvialls after existing supersonic flow breaking method broken wall, when extracting astaxanthin, extraction yield is lower.
Summary of the invention
In order to solve, adopt existing wall-breaking method to after the Haematocoocus Pluvialls broken wall, the problem that the extraction yield of astaxanthin is lower; The invention provides a kind of micronizing wall-breaking method of haematococcus pluvialis cell.
Technical scheme of the present invention:
A kind of micronizing wall-breaking method of haematococcus pluvialis cell, the steps include:
(1) by the spore of haematococcus pluvialis powder of lucifuge refrigeration lyophilize 2h under-15 ~-10 ℃, lucifuge condition;
(2) dried spore of haematococcus pluvialis powder is joined in the airtight working cavity of Ultra-Micro Grinding Equipment; Pass into liquid nitrogen in working cavity, by the air emptying in working cavity;
(3) flow velocity of adjustment liquid nitrogen is 0.2 ~ 0.4m/s, in working cavity, continues to pass into liquid nitrogen, starts Ultra-Micro Grinding Equipment, micronizing 1-3min;
(4) the haematococcus pluvialis cell powder after the collection broken wall at sample export place, lucifuge refrigeration.
The micronizing wall-breaking method of above-mentioned haematococcus pluvialis cell, preferred, the temperature of liquid nitrogen is-197 ℃.
The micronizing wall-breaking method of above-mentioned haematococcus pluvialis cell, preferred, the rotating speed of Ultra-Micro Grinding Equipment is 26000r/min.
The micronizing wall-breaking method of above-mentioned haematococcus pluvialis cell, preferred, the add-on of described spore of haematococcus pluvialis powder is 1/3 to 1/2 of working cavity capacity.
The micronizing wall-breaking method of above-mentioned haematococcus pluvialis cell, preferred, the micronizing time is 1min.
The micronizing wall-breaking method of above-mentioned haematococcus pluvialis cell, preferred, described Ultra-Micro Grinding Equipment is made with stainless material.
Method of the present invention, whole broken wall process is carried out under the protection of liquid nitrogen environment.At first, before pulverizing, pass into liquid nitrogen by the air emptying in work chamber, Haematocoocus Pluvialls is become fragile under instantaneous very low temperature effect, improved shell-broken effect.Secondly, continue to pass into liquid nitrogen in the broken wall process; Can reduce the high temperature produced in the high-speed friction collision process because of Haematocoocus Pluvialls on the one hand in working chamber, make the cell walls of Haematocoocus Pluvialls all the time in more crisp state, avoid the heat-sensitive ingredients in Haematocoocus Pluvialls destroyed simultaneously; Form on the other hand protection of the environment in working chamber, make the isolation of Haematocoocus Pluvialls and oxygen, can avoid the oxidized destruction of the bioactive ingredients such as astaxanthin in the Haematocoocus Pluvialls after broken wall; Can also reduce on the one hand again the viscosity of Haematocoocus Pluvialls, prevent that Haematocoocus Pluvialls from gluing wall.Therefore, the haematococcus pluvialis cell after employing the inventive method broken wall, the rate of release of its active substance is high and not oxidized, thereby has improved the extraction yield of active substance.
Method of the present invention can be used for the Haematocoocus Pluvialls of inhomogeneity carotene carotene content and spore thereof etc. are carried out to broken wall treatment, thereby carries out the extraction that astaxanthin, alpha-carotene, β-carotene, xenthophylls etc. have the carotene active component of physiological function.
Beneficial effect
(1) percentage of damage of Haematocoocus Pluvialls is high, degree of crushing is high and even, the treatment time is short;
(2) effectively prevent that astaxanthin isoreactivity composition is by the oxidational losses of oxygen;
(3) effectively prevent that the temperature-sensitive activeconstituents from being destroyed by high temperature in shattering process;
(4) operating process is simple, easy to operate, for a small amount of sample, can once process in short-term, also can be supporting with continuous device, sample collection apparatus etc., realize Automatic continuous production, and mechanization degree is higher, the suitable suitability for industrialized production that is applied to;
(5) can not produce and pollute sample in equipment running process, safe.
The accompanying drawing explanation
Fig. 1, the haematococcus pluvialis cell of the not broken wall of observing with electron microscope;
Fig. 2, the haematococcus pluvialis cell after the employing of observing with electron microscope method broken wall of the present invention.
Embodiment
Select the spore of haematococcus pluvialis powder of propagating artificially, content astaxanthin is 2% as raw material, select the FDV supper micron mill of large blade as Ultra-Micro Grinding Equipment.
Embodiment 1
(1) the spore of haematococcus pluvialis powder of preserving under the lucifuge condition is taken out; Under lucifuge ,-10 ℃ of conditions, lyophilize 2h; Take 200g, in opening for feed is added to working cavity, be filled with-197 ℃ of Liquid nitrogen precooler 2min; The flow velocity of adjusting liquid nitrogen is 0.2m/s, continues to pass into liquid nitrogen;
(2) open Ultra-Micro Grinding Equipment, rotating speed 26000r/min, after micronizing 1min, arrestment; Collect the spore of haematococcus pluvialis powder after obtaining broken wall in the cloth bag at sample export place.
Use electron microscope observation, the sample of handling obviously diminishes than granularity before micronized pulverization, occurs a large amount of cell wall fragments in sample.Wherein, the cell walls of 78.6% haematococcus pluvialis cell occurs damaged; Crack appears in the cell walls of 19.3% haematococcus pluvialis cell; Total percentage of damage is 97.9%.
The absorbance of the spore of haematococcus pluvialis powder after the detection broken wall is 0.811A.
Embodiment 2
(1) the spore of haematococcus pluvialis powder of preserving under the lucifuge condition is taken out; Under lucifuge ,-15 ℃ of conditions, lyophilize 2h; Take 200g, in opening for feed is added to working cavity, be filled with-197 ℃ of Liquid nitrogen precooler 2min; The flow velocity of adjusting liquid nitrogen is 0.4m/s, continues to pass into liquid nitrogen;
(2) open Ultra-Micro Grinding Equipment, rotating speed 26000r/min, after micronizing 1min, arrestment; Collect the spore of haematococcus pluvialis powder after obtaining broken wall in the cloth bag at sample export place.
Use electron microscope observation, the sample of handling obviously diminishes than granularity before micronized pulverization, occurs a large amount of cell wall fragments in sample.Wherein, the cell walls of 84.7% haematococcus pluvialis cell occurs damaged; Crack appears in the cell walls of 14.1% haematococcus pluvialis cell; Total percentage of damage is 98.8%.
The absorbance of the spore of haematococcus pluvialis powder after the detection broken wall is 0.813A.
Embodiment 3
(1) the spore of haematococcus pluvialis powder of preserving under the lucifuge condition is taken out; Under lucifuge ,-15 ℃ of conditions, lyophilize 2h; Take 200g, in opening for feed is added to working cavity, be filled with-197 ℃ of Liquid nitrogen precooler 2min; The flow velocity of adjusting liquid nitrogen is 0.4m/s, continues to pass into liquid nitrogen;
(2) open Ultra-Micro Grinding Equipment, rotating speed 26000r/min, after micronizing 1min, arrestment; Collect the spore of haematococcus pluvialis powder after obtaining broken wall in the cloth bag at sample export place.
The sample of handling obviously diminishes than granularity before micronized pulverization, occurs a large amount of cell wall fragments in sample.Wherein, the cell walls of 83.3% haematococcus pluvialis cell occurs damaged; Crack appears in the cell walls of 15.2% haematococcus pluvialis cell; Total percentage of damage is 98.5%.
The absorbance of the spore of haematococcus pluvialis powder after the detection broken wall is 0.810A.
Comparative Examples
(1) the spore of haematococcus pluvialis powder of preserving under the lucifuge condition is taken out; Under lucifuge ,-15 ℃ of conditions, lyophilize 2h; Take 200g, in opening for feed is added to working cavity;
(2) open Ultra-Micro Grinding Equipment, rotating speed 26000r/min, after micronizing 2min, arrestment; Collect the spore of haematococcus pluvialis powder after obtaining broken wall in the cloth bag at sample export place.
With the spore of haematococcus pluvialis powder after the electron microscope observation broken wall, total sporoderm-broken rate is 83.7%.The absorbance of the spore of haematococcus pluvialis powder after the detection broken wall is 0.762A.
Claims (6)
1. the micronizing wall-breaking method of a haematococcus pluvialis cell, is characterized in that, the steps include:
(1) by the spore of haematococcus pluvialis powder of lucifuge refrigeration lyophilize 2h under-15 ~-10 ℃, lucifuge condition;
(2) dried spore of haematococcus pluvialis powder is joined in the airtight working cavity of Ultra-Micro Grinding Equipment; Pass into liquid nitrogen in working cavity, by the air emptying in working cavity;
(3) flow velocity of adjustment liquid nitrogen is 0.2 ~ 0.4m/s, in working cavity, continues to pass into liquid nitrogen, starts Ultra-Micro Grinding Equipment, micronizing 1-3min;
(4) the haematococcus pluvialis cell powder after the collection broken wall at sample export place, lucifuge refrigeration.
2. the micronizing wall-breaking method of haematococcus pluvialis cell according to claim 1, is characterized in that, the temperature of liquid nitrogen is-197 ℃.
3. go up the micronizing wall-breaking method of haematococcus pluvialis cell according to claim 1 and 2, it is characterized in that, the rotating speed of Ultra-Micro Grinding Equipment is 26000r/min.
4. the micronizing wall-breaking method of haematococcus pluvialis cell according to claim 1 and 2, is characterized in that, the add-on of described spore of haematococcus pluvialis powder is 1/3 to 1/2 of working cavity capacity.
5. the micronizing wall-breaking method of haematococcus pluvialis cell according to claim 1 and 2, is characterized in that, the micronizing time is 1min.
6. the micronizing wall-breaking method of haematococcus pluvialis cell according to claim 1 and 2, is characterized in that, described Ultra-Micro Grinding Equipment is used stainless material to make.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531530A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Wall breaking method for haematococcus pluvialis |
| CN105942127A (en) * | 2016-05-30 | 2016-09-21 | 徐州工程学院 | Purple sweet potato based compound oxidation-resistant solid instant beverage and preparation method |
| CN107459870A (en) * | 2017-08-31 | 2017-12-12 | 黄太海 | A kind of method done raw material with tradition system ink, black cream is produced using wall breaking technology |
| CN109170855A (en) * | 2018-09-07 | 2019-01-11 | 福建品鉴食品有限公司 | A kind of haematococcus pluvialis instant cubilose and preparation method thereof |
| CN109419819A (en) * | 2017-09-05 | 2019-03-05 | 中国海洋大学 | A kind of haematococcus pluvialis pigment nano freeze-dried powder being soluble in cold water and its preparation and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786148A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院海洋研究所 | Wall breaking method of microalgae cell |
| CN101381337A (en) * | 2007-09-03 | 2009-03-11 | 陈锦猜 | Astaxanthin extraction method |
| CN102911095A (en) * | 2011-10-26 | 2013-02-06 | 江苏江大源生态生物科技有限公司 | Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder |
-
2013
- 2013-03-15 CN CN2013100829462A patent/CN103122313A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786148A (en) * | 2004-12-10 | 2006-06-14 | 中国科学院海洋研究所 | Wall breaking method of microalgae cell |
| CN101381337A (en) * | 2007-09-03 | 2009-03-11 | 陈锦猜 | Astaxanthin extraction method |
| CN102911095A (en) * | 2011-10-26 | 2013-02-06 | 江苏江大源生态生物科技有限公司 | Method for supercritical CO2 extraction of effective ingredients of Haematococcus pluvialis spore powder |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531530A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Wall breaking method for haematococcus pluvialis |
| CN105942127A (en) * | 2016-05-30 | 2016-09-21 | 徐州工程学院 | Purple sweet potato based compound oxidation-resistant solid instant beverage and preparation method |
| CN107459870A (en) * | 2017-08-31 | 2017-12-12 | 黄太海 | A kind of method done raw material with tradition system ink, black cream is produced using wall breaking technology |
| CN109419819A (en) * | 2017-09-05 | 2019-03-05 | 中国海洋大学 | A kind of haematococcus pluvialis pigment nano freeze-dried powder being soluble in cold water and its preparation and application |
| CN109419819B (en) * | 2017-09-05 | 2021-11-30 | 中国海洋大学 | Cold water-soluble haematococcus pluvialis pigment nano freeze-dried powder and preparation and application thereof |
| CN109170855A (en) * | 2018-09-07 | 2019-01-11 | 福建品鉴食品有限公司 | A kind of haematococcus pluvialis instant cubilose and preparation method thereof |
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Application publication date: 20130529 |