CN103092090B - Sequencing control system - Google Patents
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- CN103092090B CN103092090B CN201210339071.5A CN201210339071A CN103092090B CN 103092090 B CN103092090 B CN 103092090B CN 201210339071 A CN201210339071 A CN 201210339071A CN 103092090 B CN103092090 B CN 103092090B
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Abstract
The invention relates to the field of automatic control, and provides a sequencing control system. The sequencing control system comprises a first sequencing response unit, a first figure collection unit, a second sequencing response unit, a second figure collection unit and a control unit, wherein the first sequencing response unit is used for control over the sequencing response of a first sequencing response chamber; the first figure collection unit is used for control over signal detection and collection for the first sequencing response chamber by a figure collection component to obtain signal data; the second sequencing response unit is used for control over sequencing response of a second sequencing response chamber; the second figure collection unit is used for control over signal detection and collection for the second sequencing response chamber by the figure collection component to obtain signal data; and the control unit is used for control over multiple times of cycle operation of the first sequencing response unit and the first figure collection unit, and also use for control over multiple times of cycle operation of the second sequencing response unit and the second figure collection unit. The sequencing control system can enable rapid sequencing to be achieved, and capable of improving the use ratio of the sequencing control system.
Description
Technical field
The present invention relates to automation control area, more particularly, relate to a kind of order-checking control system.
Background technology
There is the scene of letting a hundred schools contend in current order-checking industry, there is different order-checking principles in different companies, such as: extensive parallel signature order-checking (Massively Parallel Signature Sequencing, MPSS), polonies (Polony Sequencing), 454 Manganic pyrophosphate complex initiation (454 pyrosequencing), Illumina (Solexa) sequencing, ABI SOLiD sequencing, ionic semiconductor order-checking (Ion semiconductor sequencing), DNA nanosphere order-checking (DNA nanoball sequencing) etc.Although order-checking principle is different, the process of order-checking is substantially identical, first, is fixed on by sample on solid phase carrier; Then, solid phase carrier is arranged on the sequencing reaction platform in sequencing system, forms sequencing reaction small chamber or sequence testing chip, and sequencing reagent is imported on sequencing reaction small chamber or sequence testing chip, with the sample generation sequencing reaction on solid phase carrier; Then, utilize excitation source to irradiate surface of solid phase carriers, and utilize the signal of detector detection, collection surface of solid phase carriers; Finally, the signal collected is processed, obtain the sequence information of sample.
In prior art, sequencing system comprises reagent delivery assembly, sequencing reaction assembly, adopts figure assembly and information processing component.Wherein, sequencing reaction assembly comprises attemperating unit and sequencing reaction small chamber (or sequence testing chip).The control method of this sequencing system comprises: step 1, carries out sequencing reaction, comprises reagent delivery assembly by reagent delivery to sequencing reaction small chamber, and attemperating unit controls estimating reaction small chamber temperature; Step 2, adopts figure assembly and detects the signal occurred on the sequencing reaction small chamber of sequencing reaction and collect; Circulation step 1 and step 2, until complete sequencing reaction; Step 3, information processing component processes the signal collected, and obtains the sequence information of sample.In the technical program, when carrying out step 1, adopting figure assembly and leaving unused, when carrying out step 2, reagent delivery assembly and sequencing reaction assembly leave unused, and order-checking efficiency is low on the one hand, on the other hand, causes the utilization factor of sequencing system low.
Therefore need a kind of order-checking control system newly can realize quick order-checking, and improve the utilization factor of sequencing system.
Summary of the invention
The object of the present invention is to provide a kind of order-checking control system, the low and problem that the utilization factor of system is low of the order-checking efficiency being intended to solve prior art sequencing system.
In order to realize goal of the invention, a kind of order-checking control system comprises: the first sequencing reaction unit, first adopts figure unit, the second sequencing reaction unit, second adopts figure unit and control module.
Wherein, described first sequencing reaction unit, for controlling the sequencing reaction of the first sequencing reaction small chamber; Described first adopts figure unit, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the first sequencing reaction small chamber; Described second sequencing reaction unit, for controlling the sequencing reaction of the second sequencing reaction small chamber; Described second adopts figure unit, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the second sequencing reaction small chamber; Described control module is adopted figure unit carry out repeatedly periodic duty for being controlled the first sequencing reaction unit and first, and adopts figure unit carry out repeatedly periodic duty for controlling the second sequencing reaction unit and second; Described first adopts figure unit and the second sequencing reaction unit carries out work in section at one time, and has the longest co-operation time.
Wherein, described first sequencing reaction unit for controlling reagent delivery assembly by reagent delivery to the first sequencing reaction small chamber, and to heat the first sequencing reaction small chamber for controlling attemperating unit or to freeze; Described second sequencing reaction unit for controlling reagent delivery assembly by reagent delivery to the second sequencing reaction small chamber, and to heat the second sequencing reaction small chamber for controlling attemperating unit or to freeze.
Wherein, described first adopts figure unit for controlling the leveling of the first sequencing reaction small chamber, and adopts the focusing of figure assembly to the first sequencing reaction small chamber for controlling; Described second adopts figure unit for controlling the leveling of the second sequencing reaction small chamber, and for controlling the focusing of adopting figure assembly second sequencing reaction small chamber.
In above-mentioned arbitrary technical scheme, described system also comprises information process unit, for carrying out information processing to signal data, obtains sequence fragment.
Wherein, described first adopt figure unit and comprise the first signal acquisition module and first data transmission module; Described first signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the first sequencing reaction small chamber, obtaining signal data; Described first data transmission module, for real-time Transmission signal data to information process unit.
Wherein, described second adopt figure unit and comprise secondary signal acquisition module and the second data transmission module; Described secondary signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the second sequencing reaction small chamber, obtaining signal data; Described second data transmission module, for real-time Transmission signal data to information process unit.
In order to realize goal of the invention better, the present invention also comprises a kind of order-checking control method, and the method comprises the following steps: steps A. the sequencing reaction of the first sequencing reaction unit controls first sequencing reaction small chamber; Step B. first adopts figure unit controls and adopts figure assembly and carry out input and collection to the first sequencing reaction small chamber, obtains signal data; The sequencing reaction of step C. second sequencing reaction unit controls second sequencing reaction small chamber; Step D. second adopts figure unit controls and adopts figure assembly and carry out input and collection to the second sequencing reaction small chamber, obtains signal data; Step e. repeat steps A once; Step F. repeat step B to E, until the first sequencing reaction small chamber completes all sequencing reactions; Step G. repeats step B and step C once; Step H. repeats step D once; Described step B and the step C interior execution of section at one time, and there is the longest common execution time; The interior execution of section at one time of described step D and step e, has the longest common execution time.
As from the foregoing, sequencing system of the present invention can realize carrying out parallel non-interfering order-checking to multiple sequencing reaction small chamber and control, and achieves the quick order-checking of sequencing system, improves the utilization factor of sequencing system while improving order-checking efficiency.
Accompanying drawing explanation
Fig. 1 is the structural representation of sequencing system in one embodiment of the invention.
Fig. 2 is the Control system architecture schematic diagram that checks order in one embodiment of the invention.
Fig. 3 is the process flow diagram of sequencing procedure in one embodiment of the invention.
Fig. 4 is sequencing reaction small chamber workflow diagram in one embodiment of the invention.
Fig. 5 is information process unit result figure in one embodiment of the invention.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.
In order to make follow-up embodiment be easier to understand, first the present invention provides sequence measurement.In existing sequencing technologies, a kind of sequence measurement is: utilize and connect sequencing to determine the base sequence in every bar sequence.Utilize four look fluorescence or two looks fluorescently-labeled primer to be connected with order-checking anchor primer, this fluorescently-labeled color is relevant to the base type of ad-hoc location on primer, order-checking anchor primer and the known array complementary pairing contained on the sequencing library of sequence to be measured; After each coupled reaction, utilize exciting light to irradiate, be with fluorescently-labeled point to be excited, and send exciting light, then obtain the signal at each luminescence place, can know corresponding to this light signal base that the is sequence to be measured of ad-hoc location complementation on primer by analysis.Above-mentioned sequence measurement is common practise, is not described in detail at this, specifically can see Chinese invention patent application: 201110222894.5.
For sequencing system, the present invention proposes the first embodiment.As shown in Figure 1, sequencing system can comprise: sequencing reaction small chamber 01, reagent delivery assembly 02, Control Component 03 and Cai Tu assembly 04.
(1) sequencing reaction small chamber 01 is for sequencing reaction.
(2) reagent delivery assembly 02, for according to the instruction of Control Component 04 by reagent delivery to sequencing reaction small chamber 01.
(3) Control Component 03, for controlling reagent delivery assembly 02 and Cai Tu assembly 04 carries out work.
(4) adopt figure assembly 04, for according to the instruction of Control Component 03 to sequencing reaction small chamber 01 with adopt figure.
Above-mentioned sequencing reaction small chamber 01 is the device or the carrier that can be used for arbitrarily fixed sample, such as: sequencing reaction chamber, genetic chip etc., described sequencing system comprises at least one sequencing reaction small chamber 01.Preferably, sequencing system comprises two sequencing reaction small chambers 01, is called the first sequencing reaction small chamber and the second sequencing reaction small chamber.Described reagent delivery assembly 02 can comprise: porous plate, pump and valve, and described pump and valve are by tubes connection, and valve is positioned at optional position above porous plate, pump and valve with the use of, for by the reagent aspiration in porous plate in sequencing reaction small chamber.Described reagent delivery assembly 02 also can comprise: porous plate, pump and mechanical arm, and described mechanical arm is connected with pump by conduit, and for extracting the reagent held in porous plate, pump is also connected with porous plate by conduit, extracts the reagent held in porous plate.Described Control Component 03 can comprise: host computer and lower level controller.Wherein, host computer and lower level controller carry out communication by serial ports.Described host computer is used for providing input instruction interface, can be computing machine, embedded Control panel etc.Described lower level controller is used for controlling reagent delivery assembly 02 according to the instruction of host computer and Cai Tu assembly 04 works, and can be single-chip microcomputer or PLC.The described figure of adopting assembly 04 can be comprised the multiplying arrangement for amplifying the image in spotted area in sequencing reaction small chamber and by multiplying arrangement, sequencing reaction small chamber be carried out to the bat map device of bat figure, wherein, described multiplying arrangement can be microscope, lens etc., and described bat map device can be CCD, CMOS etc.Sequencing system of the present invention is not by the restriction of the sequencing system in the present embodiment, and the sequencing system in the present embodiment is preferred embodiment.
For the order-checking control system for controlling sequencing system, the present invention proposes the second embodiment, as shown in Figure 2.Order-checking control system comprises: the first sequencing reaction unit 2, first is adopted figure unit 1, second sequencing reaction unit 3, second and adopted figure unit 4 and control module 5.Wherein: (1) first sequencing reaction unit 2, for controlling the sequencing reaction of the first sequencing reaction small chamber.(2) first adopt figure unit 1, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the first sequencing reaction small chamber.(3) second sequencing reaction unit 3, for controlling the sequencing reaction of the second sequencing reaction small chamber.(4) second adopt figure unit 4, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the second sequencing reaction small chamber.(5) control module 5 is adopted figure unit 1 carry out repeatedly periodic duty for being controlled the first sequencing reaction unit 2 and first, and adopts figure unit 4 carry out repeatedly periodic duty for controlling the second sequencing reaction unit 3 and second.Preferably, described first adopts figure unit and the second sequencing reaction unit carries out work in section at one time, and has the longest co-operation time.The technical program achieves sequencing system under the control of order-checking control system, can realize quick order-checking, and improve the utilization factor of sequencing system.
In the present embodiment, the first sequencing reaction small chamber and the second sequencing reaction small chamber simultaneously or asynchronously can carry out sequencing reaction, and the sequencing reaction small chamber occurred after sequencing reaction is adopted figure assembly and carried out input and collection (also namely adopting figure) respectively.As shown in Figure 2, control module 5 controls the first sequencing reaction unit 2, second sequencing reaction unit 3, first and adopts figure unit 1 and second and adopt figure unit 4 and carry out work.Time of input and collection with adopt figure assembly and need the number of the image gathered relevant to sequencing reaction small chamber, the number of the image of collection is more, and the time of input and collection is longer; The time of sequencing reaction is relevant with sequence measurement, and the sequencing reaction time that different sequencing reactions needs is different.So this gives different embodiments.
The time of preferred version one, input and collection is longer than the time of sequencing reaction, as the 3-1 in Fig. 3.First, control module 5 sends the instruction that the first sequencing reaction small chamber carries out sequencing reaction, first sequencing reaction unit 2 controls the first sequencing reaction small chamber according to this instruction and carries out sequencing reaction, and control module 5 detects the state of current first sequencing reaction unit 2 in real time simultaneously.After first time, sequencing reaction completed, control module 5 transmission is adopted figure instruction and is adopted figure unit 1 to first, first adopts figure unit 1 controls to adopt figure assembly and carry out input and collection to the first sequencing reaction small chamber according to adopting figure instruction, simultaneously, control module 5 sends the instruction that the second sequencing reaction small chamber carries out sequencing reaction, second sequencing reaction unit 3 controls the second sequencing reaction small chamber according to this instruction and carries out sequencing reaction, and control module 5 detects the state that first adopts figure unit 1 and the second sequencing reaction unit 3 in real time.Control module 5 monitor first adopt figure unit 1 and the second sequencing reaction unit 3 all complete instruction after (time that the sequencing reaction of the second sequencing reaction small chamber completes early than or complete with the input of the first sequencing reaction small chamber and collection) simultaneously, control module 5 sends the instruction of the second sequencing reaction small chamber being adopted to figure, second adopts figure unit 4 controls to adopt figure assembly and carry out input and collection to the second sequencing reaction small chamber according to this instruction, meanwhile, control module 5 sends the instruction that the first sequencing reaction small chamber carries out sequencing reaction, first sequencing reaction unit 2 controls the first sequencing reaction small chamber according to this instruction and carries out sequencing reaction, control module 5 detects in real time the second state of adopting figure unit 4 and the first sequencing reaction unit 2 (time that the sequencing reaction of the first sequencing reaction small chamber completes early than or simultaneously complete with the input of the second sequencing reaction small chamber and collection).As said method, the first sequencing reaction small chamber and the second sequencing reaction small chamber all circulate and carry out sequencing reaction and input, until complete order-checking.
The time of preferred version two, input and collection is shorter than the time of sequencing reaction, as the 3-2 in Fig. 3.First, control module 5 sends the instruction that the first sequencing reaction small chamber carries out sequencing reaction, first sequencing reaction unit 2 controls the first sequencing reaction small chamber according to this instruction and carries out sequencing reaction, and control module 5 detects the state of current first sequencing reaction unit 2 in real time simultaneously.After first time, sequencing reaction completed, control module sends the sequencing reaction instruction of the second sequencing reaction small chamber, second sequencing reaction unit 3 controls the second sequencing reaction small chamber according to this instruction and carries out sequencing reaction, simultaneously, control module 5 transmission is adopted figure instruction and is adopted figure unit 1 to first, first adopts figure unit 1 controls to adopt figure assembly and carry out input and collection to the first sequencing reaction small chamber according to this instruction, and control module 5 detects the state that first adopts figure unit 1 and the second sequencing reaction unit 3 in real time.Control module 5 monitor first adopt figure unit 1 and the second sequencing reaction unit 3 all complete instruction after (time that the sequencing reaction of the second sequencing reaction small chamber completes is later than or completes with the input of the first sequencing reaction small chamber and collection simultaneously), control module 5 sends the instruction the first sequencing reaction small chamber being carried out to sequencing reaction, first sequencing reaction unit 2 controls control first sequencing reaction small chamber according to this instruction and carries out sequencing reaction, meanwhile, control module 5 sends the instruction that the second sequencing reaction small chamber carries out adopting figure, second adopts figure unit 4 controls to adopt figure assembly according to this instruction and carries out input and collection (time that the sequencing reaction of the first sequencing reaction small chamber completes is later than or completes with the input of the second sequencing reaction small chamber and collection simultaneously and as above states method) to the second sequencing reaction small chamber.First sequencing reaction small chamber and the second sequencing reaction small chamber all circulate and carry out sequencing reaction and input, until complete order-checking.
Preferred version three, carry out sequencing reaction simultaneously, carry out bat figure successively after sequencing reaction completes, the 3-3 in detailed process Fig. 3.Concrete control procedure is as follows: first, control module 5 sends the instruction that sequencing reaction small chamber carries out sequencing reaction, first sequencing reaction unit 2 and the second sequencing reaction unit 3 carry out work according to this instruction simultaneously, control the sequencing reaction of the first sequencing reaction small chamber and the sequencing reaction of the second sequencing reaction small chamber respectively.Control module 5 monitors the duty of the first sequencing reaction unit 2 and the second sequencing reaction unit 3 in real time, after monitoring the first sequencing reaction unit 2 and the second sequencing reaction unit 3 and completing, control module 5 sends the instruction of the carrying out of the first sequencing reaction small chamber being adopted to figure, and first adopts figure unit 1 controls to adopt figure assembly and carry out input and collection to the first sequencing reaction small chamber according to this instruction.Control module 5 Real-Time Monitoring first adopts the duty of figure unit 1, adopt after figure unit 1 completes when monitoring the first order-checking, control module 5 sends the instruction of the second sequencing reaction small chamber being adopted to figure, and second adopts figure unit 4 controls to adopt figure assembly and carry out input and collection to the second sequencing reaction small chamber according to this instruction.
Namely the preferred version that above-mentioned Fig. 3 is corresponding can realize quick order-checking, and greatly improves the utilization factor of sequencing system, and also all parts of sequencing system is collaborative carries out work, realizes the parallel order-checking of the first sequencing reaction small chamber and the second sequencing reaction small chamber.With sequencing reaction-input and the collection of carrying out the first sequencing reaction small chamber in prior art successively, then sequencing reaction-the input of the second sequencing reaction small chamber is carried out compared with collection, figure assembly is adopted when figure is adopted to one of them sequencing reaction small chamber in technical scheme of the present invention, reagent delivery assembly, attemperating unit and another sequencing reaction small chamber realize sequencing reaction jointly, the hardware achieving sequencing system can non-stop run when checking order, the utilization factor of raising system, and improve the sequencing throughput in the unit interval.
In the present embodiment, the workflow of each sequencing reaction small chamber as shown in Figure 4, first carries out sequencing reaction, after sequencing reaction completes, carry out input and collection, and circulation is checked order, until the detection of complete paired samples.Control module 5 controls the work of sequencing system according to the instruction inputted by Control Component.In above-described embodiment, the time of input and collection can regulate as required, when needs complete fast adopt figure time, inputted the number or time order of adopting figure assembly bat figure by Control Component at every turn, control module 5 resolves the order of input, control number or the time of adopting figure assembly bat figure according to this order, thus realize completing fast adopting figure.In the technical program, not general not identical due to sequencing reaction time and Cai Tu time, when adopt the figure time far away the sequencing reaction time time, number that figure assembly adopts figure can be adopted at every turn change and adopt the figure time by arranging, reduce the gap of adopting between figure time and sequencing reaction time, thus realize uninterruptedly carrying out of sequencing reaction and Cai Tu action, improve the efficiency of order-checking.In addition, control module 5 adopts multithreading to control the job order of the first sequencing reaction small chamber and the second sequencing reaction small chamber, make the non-interfering work that the first sequencing reaction small chamber is parallel with the second sequencing reaction small chamber, substantially increase the work efficiency of sequencing system.
The first sequencing reaction unit in second embodiment is for controlling the sequencing reaction of the first sequencing reaction small chamber.After first sequencing reaction unit receives the reagent delivery instruction of control module, control reagent delivery assembly by reagent delivery to the first sequencing reaction small chamber; After first sequencing reaction unit receives the temperature instruction of control module, control attemperating unit and the first sequencing reaction small chamber is heated or freezes.Under the temperature of attemperating unit controls, the reagent in the first sequencing reaction small chamber carries out sequencing reaction.After described second sequencing reaction unit receives the reagent delivery instruction of control module, control reagent delivery assembly by reagent delivery to the second sequencing reaction small chamber; After second sequencing reaction unit receives the temperature instruction of control module, control attemperating unit and the second sequencing reaction small chamber is heated or freezes.Under the temperature of attemperating unit controls, the reagent in the second sequencing reaction small chamber carries out sequencing reaction.The different sequencing reaction conditions that during order-checking, different sequence measurement is corresponding, described sequencing reaction condition comprises: the time of reacting at the temperature of the kind of the reagent in sequencing reaction small chamber and dosage, sequencing reaction and each temperature.It should be noted that reagent transfer instruction and temperature instruction in the present invention without sequencing, also can realize reagent delivery assembly and first transmit reagent, then attemperating unit control temperature; Or attemperating unit control temperature while reagent delivery assembly transmission reagent; Or attemperating unit first carries out temperature control to sequencing reaction small chamber, reagent delivery assembly is carrying out reagent delivery.Preferably, attemperating unit control temperature while reagent delivery assembly transmission reagent, can improve the efficiency of sequencing reaction so greatly, without the need to waiting for that separately reagent delivery or temperature control.
After the first sequencing reaction small chamber completes sequencing reaction, first, namely the first leveling of adopting figure unit controls first sequencing reaction small chamber, also make the plane at the place, region of the first sequencing reaction small chamber fixed sample vertical with adopting figure assembly; Then, first adopts figure assembly controls to adopt figure assembly and focus on the first sequencing reaction small chamber, controls the exposure of adopting figure assembly simultaneously, also namely realizes input; Then, adopt figure assembly and figure is adopted to the first sequencing reaction small chamber, also namely realize signal collection.The control mode that second control mode and first of adopting figure unit adopts figure unit is identical, does not repeat them here.Wherein, the leveling of sequencing reaction small chamber, all can carry out once after each sequencing reaction completes, also can be in each sequencing procedure only after each sequencing reaction small chamber first time completes sequencing reaction, carry out one-time leveling, also namely each sequencing reaction small chamber only carries out one-time leveling in whole sequencing procedure.In the technical program, sequencing system is under first, second adopts the control of figure unit, by operations such as automatic leveling, focusing, exposures, make to adopt figure assembly and can collect data accurately in sequencing reaction small chamber, thus ensure that the accuracy of sequencing result, also ensure that the flux of order-checking simultaneously.
Order-checking control system in second embodiment also can comprise information process unit, for carrying out information processing to signal data, obtains sequence fragment.First information process unit identifies signal data, such as: the size according to signal intensity identifies.5-1 in Fig. 5 is that each bright spot represents a kind of base signal of color by the signal data that the input of adopting figure assembly obtains with collection in the present invention.When the employing four look fluorescence probe that checks order marks base, adopting figure assembly can carry out four input and collection to same position, obtains four signal datas that same base-pair on same position is answered; When the employing two look fluorescence probe that checks order marks base, when on same position, same base detects, need to carry out twice sequencing reaction, after each sequencing reaction, adopt figure assembly and twice input and collection are carried out to same position, obtain four signal datas that same base-pair on same position is answered.Below only for the analysis result of four look fluorescence probe mark bases.First information process unit tentatively identifies the signal data in same base, also namely adds up the signal intensity on four figure of shooting.In Fig. 5,5-2 is the signal data after statistics, and information process unit, again according to the signal data in 5-2, identifies the type of base.From Fig. 5,5-2 is known, and the base that base1 is corresponding to be base that A, base2 are corresponding be base that T, base3 are corresponding is base that G, base4 are corresponding is the base that A, base5 are corresponding is C, can obtain base corresponding to all signal datas according to the method.
In second embodiment, described first adopts figure unit comprises the first signal acquisition module and first data transmission module.Wherein, described first signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the first sequencing reaction small chamber, obtaining signal data; Described first data transmission module, for transmit signal data to information process unit.Adopting figure assembly to the process that the first sequencing reaction small chamber carries out input and collection is: first, first signal acquisition module controls to adopt figure assembly and focuses on the first sequencing reaction small chamber and expose, wherein, the method focused on includes but not limited to: " Z axis drift critique system " or " be dependent on the wavelet transformation of image and be defined as the function of a high pass river lower passband ", and described exposure method is without particular restriction; Then, the first signal acquisition module controls to adopt figure assembly and carries out signal collection to the first sequencing reaction small chamber, also namely adopts figure, obtains signal data; Then, the signal data adopted in figure assembly is transferred to information process unit by first data transmission module; Finally, information process unit processes signal data, obtains base sequence.Wherein, described first data transmission module can real-time Transmission signal data, also can wait for, after adopting figure assembly completes the signal collection of the first sequencing reaction small chamber, signal data is transferred to information process unit together.Described second adopts figure unit comprises secondary signal acquisition module and the second data transmission module.Described secondary signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the second sequencing reaction small chamber, obtaining signal data; Described second data transmission module, for transmit signal data to information process unit.It is identical that second working method and first of adopting figure unit adopts figure unit, can adopt figure unit, do not repeat them here with reference to first.
The present invention proposes the 3rd embodiment, based on the order-checking control method of above-mentioned order-checking control system.Described order-checking control method comprises the following steps.
Steps A. the sequencing reaction of the first sequencing reaction unit controls first sequencing reaction small chamber.Step B. first adopts figure unit controls and adopts figure assembly and carry out input and collection to the first sequencing reaction small chamber, obtains signal data.The sequencing reaction of step C. second sequencing reaction unit controls second sequencing reaction small chamber.Step D. second adopts figure unit controls and adopts figure assembly and carry out input and collection to the second sequencing reaction small chamber, obtains signal data.Step e. repeat steps A once.Step F. repeat step B to E, until the first sequencing reaction small chamber completes all sequencing reactions.Step G. repeats step B and step C once.Step H. repeats step D once.
In the present embodiment, described steps A can not perform to step H successively or perform successively.Preferably, described step B and the step C interior execution of section at one time, and there is the longest common execution time; The interior execution of section at one time of described step D and step e, has the longest common execution time.As shown in Figure 3, this is no longer going to repeat them for the idiographic flow of preferred version.
In the present embodiment, described steps A comprises: A1. first sequencing reaction unit reagent delivery assembly by reagent delivery to the first sequencing reaction small chamber; A2. the first sequencing reaction unit controls attemperating unit heats the first sequencing reaction small chamber or freezes.Preferably, described steps A 1 and steps A 2 are without sequencing.
Described step C comprises: C1. second sequencing reaction unit reagent delivery assembly by reagent delivery to the second sequencing reaction small chamber; C2. the second sequencing reaction unit controls attemperating unit heats the second sequencing reaction small chamber or freezes; Described step C1 and step C2 is without sequencing.
Preferably, described steps A 1 and steps A 2 perform successively; Described step C1 and step C2 performs successively.
Preferably, described step B and step C starts to perform or terminate simultaneously to perform simultaneously; Described step D or step e start to perform or terminate simultaneously to perform simultaneously.
Wherein, described step B comprises: B1. first sequencing reaction small chamber adopts its leveling of figure unit controls by first; B2. first adopt figure unit controls and adopt figure assembly the first sequencing reaction small chamber is focused on; B3. adopt figure assembly and input and collection are carried out to the first sequencing reaction small chamber, obtain signal data.
Wherein, described step D comprises: D1. second sequencing reaction small chamber adopts its leveling of figure unit controls by second; D2. second adopt figure unit controls and adopt figure assembly the second sequencing reaction small chamber is focused on; D3. adopt figure assembly and input and collection are carried out to the second sequencing reaction small chamber, obtain signal data.
Described order-checking control system also comprises information process unit, carrying out information processing, obtaining sequence fragment for performing I. to signal data; After described step I is positioned at step B and step D simultaneously, or after being only positioned at step H; Also comprise in described step B and step D and real-time Transmission is carried out to signal data.
Existing second generation high-flux sequence method all can adopt technical scheme of the present invention to check order, and includes but not limited to connect sequencing, synthesis sequencing.
It should be noted that the present invention typically applies but is not limited to automation control area, in other similar field, also can apply control system set forth in the present invention.
In addition, above-mentioned control system is not only for the sequencing system in the embodiment of the present invention, and the sequencing system in prior art with two sequencing reaction small chambers all can use control system of the present invention.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. check order a control system, and described order-checking control system, for controlling sequencing system; It is characterized in that,
Described sequencing system comprises two sequencing reaction small chambers, a reagent delivery assembly, a Control Component and adopt figure assembly;
Described sequencing reaction small chamber, for sequencing reaction, is called the first sequencing reaction small chamber and the second sequencing reaction small chamber;
Described reagent delivery assembly, for according to the instruction of Control Component by reagent delivery to sequencing reaction small chamber;
Described Control Component, for controlling reagent delivery assembly and Cai Tu assembly carries out work;
Describedly adopt figure assembly, for the instruction according to Control Component, figure is adopted to sequencing reaction small chamber;
Described order-checking control system comprises that the first sequencing reaction unit, first adopts figure unit, the second sequencing reaction unit, second adopts figure unit and control module;
Described first sequencing reaction unit, for controlling the sequencing reaction of the first sequencing reaction small chamber;
Described first adopts figure unit, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the first sequencing reaction small chamber;
Described second sequencing reaction unit, for controlling the sequencing reaction of the second sequencing reaction small chamber;
Described second adopts figure unit, carrying out input and collection, obtaining signal data for controlling to adopt figure assembly to the second sequencing reaction small chamber;
Described control module is adopted figure unit carry out repeatedly periodic duty for being controlled the first sequencing reaction unit and first, and adopts figure unit carry out repeatedly periodic duty for controlling the second sequencing reaction unit and second;
Described first adopts figure unit and the second sequencing reaction unit carries out work in section at one time, and has the longest co-operation time.
2. order-checking control system according to claim 1, it is characterized in that, described first sequencing reaction unit for controlling reagent delivery assembly by reagent delivery to the first sequencing reaction small chamber, and to heat the first sequencing reaction small chamber for controlling attemperating unit or to freeze;
Described second sequencing reaction unit for controlling reagent delivery assembly by reagent delivery to the second sequencing reaction small chamber, and to heat the second sequencing reaction small chamber for controlling attemperating unit or to freeze.
3. order-checking control system according to claim 1, is characterized in that, described first adopts figure unit for controlling the leveling of the first sequencing reaction small chamber, and adopts the focusing of figure assembly to the first sequencing reaction small chamber for controlling;
Described second adopts figure unit for controlling the leveling of the second sequencing reaction small chamber, and for controlling the focusing of adopting figure assembly second sequencing reaction small chamber.
4. order-checking control system according to any one of claim 1 to 3, is characterized in that, described order-checking control system also comprises information process unit, for carrying out information processing to signal data, obtains sequence fragment.
5. order-checking control system according to claim 4, is characterized in that, described first adopts figure unit comprises the first signal acquisition module and first data transmission module;
Described first signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the first sequencing reaction small chamber, obtaining signal data;
Described first data transmission module, for real-time Transmission signal data to information process unit.
6. order-checking control system according to claim 4, is characterized in that, described second adopts figure unit comprises secondary signal acquisition module and the second data transmission module;
Described secondary signal acquisition module, carrying out input and collection for controlling to adopt figure assembly to the second sequencing reaction small chamber, obtaining signal data;
Described second data transmission module, for real-time Transmission signal data to information process unit.
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| CN201210339071.5A CN103092090B (en) | 2012-09-14 | 2012-09-14 | Sequencing control system |
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| CN2478110Y (en) * | 2001-04-25 | 2002-02-20 | 张宏 | Full automatic gene sequencing instrument |
| CN1408884A (en) * | 2001-09-22 | 2003-04-09 | 香港基因晶片开发有限公司 | Device and method for detecting gene sequence |
| CN102146442A (en) * | 2010-12-31 | 2011-08-10 | 深圳华因康基因科技有限公司 | Method and system for controlling sequencing process of gene sequencer |
| CN102174384A (en) * | 2011-01-05 | 2011-09-07 | 深圳华因康基因科技有限公司 | Method and system for controlling sequencing and signal processing of gene sequencer |
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| US7244559B2 (en) * | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US20060188899A1 (en) * | 2004-10-07 | 2006-08-24 | Dewalch N B | High speed DNA sequencer and method |
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| CN2478110Y (en) * | 2001-04-25 | 2002-02-20 | 张宏 | Full automatic gene sequencing instrument |
| CN1408884A (en) * | 2001-09-22 | 2003-04-09 | 香港基因晶片开发有限公司 | Device and method for detecting gene sequence |
| CN102146442A (en) * | 2010-12-31 | 2011-08-10 | 深圳华因康基因科技有限公司 | Method and system for controlling sequencing process of gene sequencer |
| CN102174384A (en) * | 2011-01-05 | 2011-09-07 | 深圳华因康基因科技有限公司 | Method and system for controlling sequencing and signal processing of gene sequencer |
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