CN103055968A - Oil-water-separation micro-fluidic chip and preparation method thereof - Google Patents

Oil-water-separation micro-fluidic chip and preparation method thereof Download PDF

Info

Publication number
CN103055968A
CN103055968A CN 201210586896 CN201210586896A CN103055968A CN 103055968 A CN103055968 A CN 103055968A CN 201210586896 CN201210586896 CN 201210586896 CN 201210586896 A CN201210586896 A CN 201210586896A CN 103055968 A CN103055968 A CN 103055968A
Authority
CN
Grant status
Application
Patent type
Prior art keywords
oil
water
micro
separation
chip
Prior art date
Application number
CN 201210586896
Other languages
Chinese (zh)
Inventor
沙俊
Original Assignee
苏州汶颢芯片科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Abstract

The invention relates to an oil-water-separation micro-fluidic chip and a preparation method thereof. A micro-structure and a micro-channel are arranged on the surface of the micro-fluidic chip, and the left side and the right side of the micro-channel respectively carry out hydrophilic and oleophylic modification. Under the action of an electric field, an oil-water mixed solution is demulsified, water moves to the hydrophilic side, oil moves to the oleophylic side, and finally oil water-separation is realized. The oil-water-separation micro-fluidic chip is mainly applied to the related fields of transformer oil, turbine oil of power plants, mrine oil spill processing, failed oil of oil fields, oil spill of offshore drilling platforms, advective oil of steel mills, a lot of oily wastewater of oil refineries and the like. The micro-fluidic chip realizes the rapid demulsification and rapid separation of the oil-water mixed solution, has the characteristics of convenience, economy, rapidness and high efficiency, and provides a new analysis technique for the field of oil-water separation.

Description

一种油水分离的微流控芯片及其制备方法技术领域[0001] 本发明涉及一种油水分离的微流控芯片及其制备方法,该微流控芯片表面有微结构和微通道,在微通道的左右两侧分别进行亲水和亲油修饰,在电场的作用下,油水混合溶液发生破乳,水往亲水侧移动,油往亲油侧移动,最后实现油水分离,主要应用于变压器油、 发电厂透平油、海上原油泄漏事故处理、油田落地油、海上钻井平台浮油、钢铁厂平流油、炼油厂大量含油废水等相关领域。 One kind of oil-water separation microfluidic chip and TECHNICAL FIELD [0001] The present invention relates to a micro-fluidic chip and a method for preparing oil-water separation, the microfluidic chip surface microstructures and microchannels in the micro- left and right channels are respectively hydrophilic and lipophilic modified, under the action of an electric field, the occurrence of breaking oil-water mixed solution, hydration of the hydrophilic side to move, oil moves towards lipophilic side, finally to achieve water separation, mainly used in transformer oil , power plant turbine oil, offshore oil spill treatment, ground oil fields, the oil slick offshore drilling platforms, steel mills stratospheric oil refinery a lot of oily wastewater and other related fields. 背景技术[0002] 20世纪80年代后期,为了建立更类似于体内环境的培养体系,尽可能使体外环境与体内环境相吻合,从而使细胞间能相互沟通信息,相互支撑生长增殖,人们在细胞培养技术的基础上发展出了细胞共培养技术。 [0002] 20 in the late 1980s, in order to establish a training system more similar to the internal environment, as far as possible the in vitro environment and internal environment is consistent, so that cells can communicate information between each other, mutual support growth and proliferation of cells in people the development of basic technical training on the cell co-culture techniques. 细胞共培养技术是将2种或2种以上的细胞共同培养于同一环境中,由于其具有更好地反映体内环境的优点,所以这种方法被广泛应用于现代细胞研究中。 Cell co-culture techniques is one or two or more cells co-cultured in the same environment, since it has the advantage of better reflect the in vivo environment, so this method is widely used in modern cell research. [0003]目前,细胞共培养技术最多应用于骨细胞和神经细胞。 [0003] At present, most cell co-culture technology in bone and nerve cells. 细胞共培养体系主要通过两种方法建立:①直接共培养体系,即将2种或2种以上的细胞同时或分别接种于同一孔中,不同种类的细胞之间直接接触;②间接共培养体系,即将2种或2种以上的细胞分别接种于不同的载体上,然后将这两种载体置于同一培养环境之中,使不同种类的细胞共用同一种培养体系而不直接接触。 Coculture established mainly two methods: ① direct co-culture system, is about two or more kinds of cells were inoculated simultaneously or in the same well, the direct contact between the different types of cells; ② indirect co-culture system, is imminent or more kinds of two kinds of cells were seeded on different carriers, then these two vectors placed in the same culture environment in which different types of cells sharing the same without directly contacting the culture system. [0004] 共培养体系主要作用:诱导细胞向另一种细胞分化;诱导细胞自身分化;维持细胞功能和活力;调控细胞增殖;促进早期胚胎发育和提高代谢产物产量。 [0004] The primary role of co-culture system: induce cell differentiation to another cell; itself induce cell differentiation; regulation of cell proliferation;; maintain cell function and viability promote early embryonic development and increase the metabolic product yield. 从上世纪30年代开始,细胞培养逐渐成为研究人员实验过程中不可缺少的重要步骤,其载体工具:培养皿/ 培养瓶也逐步被大家所认可,成为了一种常规的实验耗材。 From the beginning of the 1930s, cell culture experiment researchers gradually become an important and indispensable step in its support tools: dish / flasks are also gradually being recognized by everyone, it has become a routine laboratory supplies. 虽然到目前为止,很多科学家认为培养皿/培养瓶的这种体外培养条件与体内生长环境有着显著的不同,但是由于没有更好的培养载体来改变这个现状,所以生物学家们也只能退而求次的默认这种情况的存在。 Although so far, many scientists believe that dish / flasks of this culture conditions in vitro and in vivo growth environment has a significant difference, but the lack of a better culture carrier to change the status quo, so biologists can only retreat and there is a default in this case seek times. 但是近来,一种Petaka细胞培养系统的问世,获得美国、欧洲广大生物科学家的青睐,培养皿/培养瓶的一些致命缺陷又再一次被提及,成为研究人员热烈讨论的话题之一。 But recently, the advent of one kind Petaka cell culture system, the US, Europe favor the majority of biological scientists, dish / flasks, some fatal flaw once again been mentioned as one of the researchers topic of lively discussion. [0005] 常规的细胞培养皿很难完成多种细胞的共培养,因此,发展一种便捷、快速、高效、 低成本的多细胞培养技术,是细胞生物学等领域的迫切需求。 [0005] Conventional cell culture dish more difficult to achieve co-cultured cells, and therefore, the development of a convenient, fast, efficient, low-cost multi-cell culture technology is an urgent demand in the field of cell biology. 近年来,微流控芯片分析技术已成为分析化学中一个重要的研究方向,是其中最活跃的一支,无论是在科研还是应用领域都获得了广泛的重视。 In recent years, microfluidic analysis technology has become an important analytical chemistry research, it is one of the most active one, both in research or applications have gained widespread attention. 微流控芯片作为一种新型的分析检测平台,具有高通量、集成化、 多重平行分析、便携式、易操作、成本低等优点,已经在众多领域获得了广泛应用。 Microfluidic chip as a new platform for analysis and detection, high throughput, integrated, multiple parallel analysis, portable, easy to operate, low cost, has been widely used in many fields. 然而,采用微流控芯片,在其表面制备微结构和微通道,依靠微通道中多层液体之间的层流效应驱动样品微流体,同时完成多种细胞的植入技术,目前在多种细胞共培养的应用领域尚未有实质性的突破。 However, with the microfluidic chip, in its micro-structure and micro channel surface preparation, depend on flow effect between the layers of the multilayered liquid microchannels of microfluidic sample drive, while the completion of more cells implantation techniques, various currently cell co-culture of the applications have not been substantive breakthrough. [0006] 多种细胞共培养微流控芯片能将2种或2种以上的细胞共同培养于同一环境中, 能更真实地反映人体组织细胞之间的互相影响,有利于实验者观察细胞与细胞之间互相作用,特别是多个细胞对一个细胞的影响,也有利于快速筛选新药的疗效和毒性。 [0006] more coculture microfluidic chip capable of two or more kinds of cells co-cultured in the same environment, more truly reflect the mutual influence between human tissue cells, and the cells were observed facilitate experimenter interaction, particularly those affecting cell between multiple cells to a cell, but also conducive to the rapid screening of drug efficacy and toxicity. 发明内容[0007] 本发明的目的是提供了一种油水分离的微流控芯片及其制备方法,该微流控芯片表面有微结构和微通道,在微通道的左右两侧分别进行亲水和亲油修饰,在电场的作用下, 油水混合溶液发生破乳,水往亲水侧移动,油往亲油侧移动,最后实现油水分离。 SUMMARY OF THE INVENTION [0007] The object of the present invention is to provide a microfluidic chip and method for preparing oil-water separation, the microfluidic chip surface microstructures and microchannels, hydrophilic left and right sides, respectively, of the microchannel and lipophilic modified, under the action of an electric field, the occurrence of breaking oil-water mixed solution, the water moves to the side of a hydrophilic, lipophilic side toward the oil transfer, to achieve the final oil-water separation. 微流控芯片由刻有微米级别的微结构和微通道的芯片和粘性薄膜封合而成,微结构和微通道通过微加工技术制备。 Microfluidic chip by the engraved micron microstructure and micro-channel chips and the adhesive membrane sealed together, the microstructure and the microchannel prepared by micro-machining techniques. [0008] 为实现上述目的,本发明采用以下的操作步骤:[0009] (I)用计算机辅助设计软件设计和绘制微流控芯片中各层芯片的微结构和微通道图形。 [0008] To achieve the above object, the present invention employs the following steps: [0009] (I) and the design drawing microfluidic microstructure and micro-channel chip graphics controller chip in the layers of computer-aided design software. [0010] (2)通过微加工技术在各层微流控芯片基材表面和粘性薄膜上加工所需的微结构和微通道,包括进样孔、分离主通道和分离分通道。 [0010] (2) by micromachining techniques required for processing layers on a surface of the substrate and the microfluidic chip adhesive membrane microstructures and microchannels, including the injection hole, and the separation of the main channel separated sub-channel. [0011] (3)利用双层粘性薄膜,将各层离心式微流控芯片对齐、粘合、加压封合,组成油水分离的微流控芯片。 [0011] (3) by the double adhesive film, the microfluidic chip alignment layers centrifugation, adhesive, pressure sealing, consisting of oil-water separation microfluidic chip. [0012] (4)将油水混合溶液从样品池加入,施加一定电场,使油水混合溶液进行破乳。 [0012] (4) from the oil-water mixture was added to the sample cell, applying a constant electric field, so that demulsification of oil and water mixed solution. [0013] (5)溶液迅速破乳后,利用亲水和亲油界面的同性相吸原理,完成油水的分离。 [0013] (5) After the solution was rapidly breaking, with a hydrophilic and lipophilic interface like attracts principle, complete separation of oil and water. [0014] 本发明中,油水分离的微流控芯片的芯片基材可以是PMMA、PC、PVC、C0C、铜、铝、不锈钢、硅片、玻璃圆片,也可是市售的各类普通CD光盘。 [0014] In the present invention, the oil-water separation microfluidic chip substrate chip may be PMMA, PC, PVC, C0C, copper, aluminum, stainless steel, silicon wafer, glass wafer, but also various types of commercially available ordinary CD CD. [0015] 本发明中,油水分离的微流控芯片和粘性薄膜的微结构和微通道可以通过数控铣刻、激光刻蚀、LIGA技术、模塑法、热压法、化学腐蚀制备,也可用软刻蚀技术制备。 [0015] In the present invention, the oil-water separation microstructures and microchannels and the microfluidic chip adhesive film may be cut by CNC milling, laser etching, the LIGA technology, molding, pressing, preparing chemical etching, can also be used preparation of soft lithography. [0016] 本发明中,油水分离的微流控芯片是由两层芯片组成,各层芯片之间用粘性薄膜贴合,粘性薄膜可以是双层力致粘性薄膜,也可是普通双面胶薄膜。 [0016] In the present invention, the oil-water separation microfluidic chips is composed of two layers, between the layers of adhesive film bonded chips, the adhesive film may be a double adhesive film actuator force, but also the ordinary double-sided adhesive film . [0017] 本发明中,油水分离的微流控芯片上的样品溶液的驱动依靠油水溶液储液罐与进样孔之间的液差所产生的重力。 [0017] In the present invention, the oil-water separation of the sample solution on the driven microfluidic chip relies on a difference between the fluid reservoir and the inlet of oil wells an aqueous solution produced by gravity. [0018] 本发明中,油水分离的微流控芯片的油水破乳是依靠微通道两侧之间形成的电场来完成的。 [0018] In the present invention, the water demulsifying oil-water separation microfluidic chip is to rely on an electric field formed between both sides of the microchannels to complete. [0019] 本发明中,油水分离的微流控芯片的微通道两侧的表面分别进行了亲水和亲油的表面修饰。 [0019] In the present invention, the surface of the water on both sides of the microchannel of the microfluidic chip separation were surface-modified hydrophilic and lipophilic. [0020] 本发明中,油水分离的微流控芯片进行微通道表面修饰的亲水剂是[0021] 本发明中,油水分离的微流控芯片进行微通道表面修饰的亲油剂是[0022] 本发明提出的油水分离的微流控芯片及其制备方法,操作简单、实现了油水混合溶液的快速破乳和迅速分离,降低了试剂与样品的用量,简化了分离过程,具有便携、经济、 快速、高效的特点,在油水分离的相关领域中具有良好的应用前景。 [0020] In the present invention, the oil-water separation microfluidic microchannel chip surface-modified hydrophilic agent is [0021] the present invention, the oil-water separation microfluidic chip surface of the microchannel is modified pro-oil [0022 ] the microfluidic chip of the oil-water separation and preparation method proposed by the present invention, simple operation, to achieve a rapid breaking of oil-water mixture solution and quickly separated, to reduce the amount of reagent and sample to simplify the separation process, portability, economical fast, efficient features, has a good prospect in the relevant field of oil-water separation. 附图说明[0023] 图1.油水分离的微流控芯片的结构示意图。 BRIEF DESCRIPTION OF schematic configuration [0023] FIG. 1. The water separator microfluidic chip. [0024] a.亲水界面,b.亲油界面,c.电场正极,d.电场负极,e.电源。 [0024] a. A hydrophilic interface, b. Lipophilic interface, c. A positive electric field, d. A negative electric field, e. Power. 具体实施方案[0025] 实施例1[0026] 用计算机辅助设计软件设计和绘制离心式微流控芯片的两层芯片的微结构和微通道图形。 Specific embodiments [0025] Example 1 [0026] Design and draw graphics microstructure and centrifuged two microchannel chip microfluidic chip using computer aided design software. 利用数控CNC系统加工制备两层圆片状聚甲基丙烯酸甲酯(PMMA)芯片的微结构和微通道,分别用自来水、蒸馏水清洗各层芯片,并用乙醇擦拭芯片表面残留的指纹、油溃等污溃。 NC CNC machining system prepared using two disc-shaped polymethyl methacrylate (PMMA) and microstructure chip microchannels, respectively, tap water, distilled water layers chip, and the chip with ethanol wipe fingerprints remaining on the surface, like oil collapse sewage collapse. 在双面胶薄膜上,用刻字机加工制备所需的微结构和微通道。 On the double-sided adhesive film, with a cutting plotter process for preparing the desired microstructure and microchannels. 将两层芯片小心对齐、粘合、加压封合,制成油水分离的微流控芯片。 The two layers were carefully aligned with the chip, adhesive, pressure sealing, made of oil-water separation microfluidic chip. 将油水溶液储液罐与进样注入孔相连, 利用液差加入待分离的油水混合溶液,施加电场,油水混合溶液在分离主通道迅速破乳,水组分向亲水界面移动,油组分向亲油界面移动,最后实现油水分离。 The oil and aqueous sample reservoir is connected to the injection hole, using a mixed solution of water were added to the difference to be separated, an electric field is applied, the mixed solution was separated oil demulsifying main channel quickly, the water moves to the hydrophilic interface component, the oil component lipophilic interface moves to finally realize the oil-water separation. [0027] 实施例2[0028] 用计算机辅助设计软件设计和绘制离心式微流控芯片的两层芯片的微结构和微通道图形。 [0027] Example 2 [0028] The microstructures and microchannels graphic design and rendering centrifugal microfluidic chip using computer aided design software embodiment of the two chips. 利用数控CNC系统加工制备两层圆片状聚碳酸酯(PC)芯片的微结构和微通道, 分别用自来水、蒸馏水清洗各层芯片,并用乙醇擦拭芯片表面残留的指纹、油溃等污溃。 NC CNC machining system prepared using two disc-shaped polycarbonate (PC) and microstructure chip microchannels, respectively, tap water, distilled water chip layers, and washed with ethanol fingerprints, dirt, etc. collapse crushed oil remaining on the surface wiped chip. 在双面胶薄膜上,用刻字机加工制备所需的微结构和微通道。 On the double-sided adhesive film, with a cutting plotter process for preparing the desired microstructure and microchannels. 将两层芯片小心对齐、粘合、加压封合,制成油水分离的微流控芯片。 The two layers were carefully aligned with the chip, adhesive, pressure sealing, made of oil-water separation microfluidic chip. 将油水溶液储液罐与进样注入孔相连,利用液差加入待分离的油水混合溶液,施加电场,油水混合溶液在分离主通道迅速破乳,水组分向亲水界面移动,油组分向亲油界面移动,最后实现油水分离。 The oil and aqueous sample reservoir is connected to the injection hole, using a mixed solution of water were added to the difference to be separated, an electric field is applied, the mixed solution was separated oil demulsifying main channel quickly, the water moves to the hydrophilic interface component, the oil component lipophilic interface moves to finally realize the oil-water separation.

Claims (10)

  1. 1. 一种油水分离的微流控芯片及其制备方法,其特征在于该微流控芯片表面有微结构和微通道,在微通道的左右两侧分别进行亲水和亲油修饰,在电场的作用下,油水混合溶液发生破乳,水往亲水侧移动,油往亲油侧移动,最后实现油水分离。 An oil-water separation microfluidic chip and a preparation method, wherein the micro-fluidic chip surface microstructures and microchannels, hydrophilic and lipophilic modified left and right sides of the microchannel, respectively, the electric field under the action, the occurrence of breaking oil-water mixed solution, the water moves to the hydrophilic side, moved in the lipophilic oil side, and finally to achieve oil-water separation. 微流控芯片由刻有微米级别的微结构和微通道的芯片和粘性薄膜封合而成,微结构和微通道通过微加工技术制备。 Microfluidic chip by the engraved micron microstructure and micro-channel chips and the adhesive membrane sealed together, the microstructure and the microchannel prepared by micro-machining techniques.
  2. 2.按权利要求1所述的油水分离的微流控芯片及其制备方法,其特征在于,其制作步骤如下:(1)用计算机辅助设计软件设计和绘制微流控芯片中各层芯片的微结构和微通道图形。 2. The microfluidic chip and method of preparation of the oil-water separator as claimed in claim 1, characterized in that its production steps as follows: (1) using computer-aided design and software design drawing microfluidic chip in the layers microstructures and microchannels pattern. (2)通过微加工技术在各层微流控芯片基材表面和粘性薄膜上加工所需的微结构和微通道,包括进样孔、分离主通道和分离分通道。 (2) by micromachining techniques required for processing layers on a surface of the substrate and the microfluidic chip adhesive membrane microstructures and microchannels, including the injection hole, and the separation of the main channel separated sub-channel. (3)利用双层粘性薄膜,将各层离心式微流控芯片对齐、粘合、加压封合,组成油水分离的微流控芯片。 (3) by the double adhesive film, the microfluidic chip alignment layers centrifugation, adhesive, pressure sealing, consisting of oil-water separation microfluidic chip. (4)将油水混合溶液从样品池加入,施加一定电场,使油水混合溶液进行破乳。 (4) from the oil-water mixture was added to the sample cell, applying a constant electric field, so that demulsification of oil and water mixed solution. (5)溶液迅速破乳后,利用亲水和亲油界面的同性相吸原理,完成油水的分离。 (5) solution was rapidly breaking, using the principle of like attracts interface hydrophilic and lipophilic, oil-water separation is accomplished.
  3. 3.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片的核心功能器件是微流控芯片,此芯片以液差产生的重力作为油水混合溶液流动的驱动力,可以批量生产、多次利用、灵活设计与组装。 Microfluidic chip and preparation method 3. The oil-water separation according to claim 1 or 2, characterized in that the core of this oil-water separation functional device chip is a micro-fluidic chip, this chip gravity difference generated in the liquid as the driving force of the flow of oil-water mixture solution, it can be mass produced, using multiple, flexible design and assembly.
  4. 4.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片上的微结构和微通道是通过数控铣刻、激光刻蚀、LIGA技术、模塑法、热压法、化学腐蚀、软刻蚀技术的微加工方法在芯片基材表面制备,尺寸在微米级别。 Microfluidic chip, and oil-water separator 4. The method for preparing according to claim 1 or 2, characterized in that the microstructures and microchannels in the oil-water separation of such chips is engraved by CNC milling, laser etching, the LIGA technology, molding, pressing, chemical etching, soft lithography in microfabrication of preparing the surface of the chip substrate, the size in the micron level.
  5. 5.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片是由两层芯片叠加而成,构成三维立体的微结构和微通道网络。 The microfluidic chip and 5. The method for preparing oil-water separation according to claim 1 or 2, characterized in that the oil-water separator which is a superposition of two chips from the chip, constituting the three-dimensional microstructures and microchannels The internet.
  6. 6.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片可以在一块芯片上制作多组微结构和微通道,构成多组油水分离单元,可同时分离多组油水混合溶液,提高了单位时间的平行油水分离能力。 Microfluidic chip, and oil-water separator 6. The preparation method according to claim 1 or 2, characterized in that the oil-water separation of such chips can produce multiple sets of microstructures and microchannels on a chip, multiple sets water a separation unit separating a plurality of sets may be simultaneously mixed solution of water, oil and water separation capacity improved parallel per unit time.
  7. 7.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片通过施加一定的电场完成分离主通道中油水混合溶液的破乳。 Microfluidic chip, and oil-water separator 7. The method for preparing according to claim 1 or 2, characterized in that the oil-water separation of such chips by applying a certain electric field is separated from the main channel to complete breaking of oil-water mixed solution.
  8. 8.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片对分离主通道的两侧进行了亲水和亲油修饰处理,利用同性相吸的原理,进行油水分离。 Microfluidic chip, and oil-water separator 8. The preparation method according to claim 1 or 2, characterized in that, on both sides of this oil-water separation chips were the main channel separated hydrophilic and lipophilic modification treatment by sex the principle of attraction, oil-water separation.
  9. 9.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片适合油包水混合溶液和水包油混合溶液的油水分离。 Microfluidic chip and preparation method as claimed in claim 9 or claim 12 oil-water separation, characterized in that the oil-water separation chip for this oil-water mixed solution a mixed solution of water and water-in-oil separation.
  10. 10.按权利要求1或2所述的油水分离的微流控芯片及其制备方法,其特征在于,这种油水分离芯片具有便携、经济、快速、高效、平行分离能力高、样品交叉污染几率小,在油水分离所涉及的众多相关领域具有广泛的应用前景。 Microfluidic chip, and oil-water separator 10. The preparation method according to claim 1 or 2, characterized in that the oil-water separation chip having such a portable, economical, fast, efficient and parallel high separation capacity, the probability of cross-contamination of samples small, with a wide range of applications in many fields related to oil-water separation involved.
CN 201210586896 2012-12-31 2012-12-31 Oil-water-separation micro-fluidic chip and preparation method thereof CN103055968A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201210586896 CN103055968A (en) 2012-12-31 2012-12-31 Oil-water-separation micro-fluidic chip and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201210586896 CN103055968A (en) 2012-12-31 2012-12-31 Oil-water-separation micro-fluidic chip and preparation method thereof

Publications (1)

Publication Number Publication Date
CN103055968A true true CN103055968A (en) 2013-04-24

Family

ID=48099035

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201210586896 CN103055968A (en) 2012-12-31 2012-12-31 Oil-water-separation micro-fluidic chip and preparation method thereof

Country Status (1)

Country Link
CN (1) CN103055968A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104312616A (en) * 2014-10-11 2015-01-28 天津大学 Method and device for realizing Pickering emulsion breaking by coupling electric fields and micro-channel

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009012112A1 (en) * 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
CN102059161A (en) * 2009-11-18 2011-05-18 中国科学院化学研究所 Microfluidic chip and manufacturing method thereof
US20120012299A1 (en) * 2010-07-16 2012-01-19 Industrial Idea Partners, Inc. Proportional Micro-Valve With Thermal Feedback
CN102784673A (en) * 2012-08-13 2012-11-21 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009012112A1 (en) * 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
CN102059161A (en) * 2009-11-18 2011-05-18 中国科学院化学研究所 Microfluidic chip and manufacturing method thereof
US20120012299A1 (en) * 2010-07-16 2012-01-19 Industrial Idea Partners, Inc. Proportional Micro-Valve With Thermal Feedback
CN102784673A (en) * 2012-08-13 2012-11-21 苏州汶颢芯片科技有限公司 Centrifugal micro-fluidic chip for detecting oil and grease and preparation method of centrifugal micro-fluidic chip

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
丁锐等: "选择性紫外曝光法修饰微流控芯片用于制备高度均一的单乳液和复乳液", 《过程工程学报》, vol. 11, no. 3, 30 June 2011 (2011-06-30) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104312616A (en) * 2014-10-11 2015-01-28 天津大学 Method and device for realizing Pickering emulsion breaking by coupling electric fields and micro-channel
CN104312616B (en) * 2014-10-11 2016-08-03 天津大学 And electric field couples microchannels implemented method and apparatus for breaking emulsions of Pickering

Similar Documents

Publication Publication Date Title
VanDelinder et al. Separation of plasma from whole human blood in a continuous cross-flow in a molded microfluidic device
Yi et al. Characterization of electrowetting actuation on addressable single-side coplanar electrodes
Warkiani et al. Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells
Fu et al. An integrated microfabricated cell sorter
Laurell et al. Chip integrated strategies for acoustic separation and manipulation of cells and particles
Di Carlo et al. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation
Weibel et al. Torque-actuated valves for microfluidics
Cho et al. Passively driven integrated microfluidic system for separation of motile sperm
Nge et al. Advances in microfluidic materials, functions, integration, and applications
Kovarik et al. Micro total analysis systems for cell biology and biochemical assays
West et al. Micro total analysis systems: latest achievements
Lin et al. A fast prototyping process for fabrication of microfluidic systems on soda-lime glass
Radisic et al. Micro-and nanotechnology in cell separation
Culbertson et al. Micro total analysis systems: fundamental advances and biological applications
Hardt et al. Microfluidic technologies for miniaturized analysis systems
Nilsson et al. Review of cell and particle trapping in microfluidic systems
Choi et al. Digital microfluidics
US20090269767A1 (en) Microfluidic chip devices and their use
Lin et al. A rapid three-dimensional vortex micromixer utilizing self-rotation effects under low Reynolds number conditions
Barrett et al. Dielectrophoretic manipulation of particles and cells using insulating ridges in faceted prism microchannels
Iliescu et al. Fabrication of a dielectrophoretic chip with 3D silicon electrodes
Bhattacharyya et al. Thermoplastic microfluidic device for on-chip purification of nucleic acids for disposable diagnostics
Li et al. Transport, manipulation, and reaction of biological cells on-chip using electrokinetic effects
Zare et al. Microfluidic platforms for single-cell analysis
Lagus et al. A review of the theory, methods and recent applications of high-throughput single-cell droplet microfluidics

Legal Events

Date Code Title Description
C06 Publication
C10 Entry into substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)