CN103040896B - Preparation method of maca extractive - Google Patents

Preparation method of maca extractive Download PDF

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CN103040896B
CN103040896B CN201310003453.5A CN201310003453A CN103040896B CN 103040896 B CN103040896 B CN 103040896B CN 201310003453 A CN201310003453 A CN 201310003453A CN 103040896 B CN103040896 B CN 103040896B
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extract
recrement
ethanol
root powder
pueraria root
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CN103040896A (en
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王亚明
焦慧良
袁勇
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YUNNAN SHENGCAOFENG BIOTECHNOLOGY CO Ltd
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Abstract

The invention provides a preparation method of a maca extractive. The method comprises the steps that maca powder is placed into a microwave dryer, heated to 50-60 DEG C for 15-25min, and cooled to a room temperature; enzyme deactivation treatment is conducted; enzyme activity is destroyed; glucosinolate hydrolysis is prevented; backflow extraction is conducted; water is added to an extractive; the extractive is stirred, stands and is precipitated; supernatant liquid is separated and concentrated; beta-cyclodextrin is added; stirring is conducted for 50-70min; the extractive is embedded; volatilization of glucosinolate is prevented; vacuum drying is conducted; and the maca extractive is obtained. The method can effectively prevent hydrolysis of an available component, namely glucosinolate, in maca, and in particular can effectively prevent volatilization of glucosinolate as the extractive is embedded at normal temperature and pressure; the component is reserved to the greatest extent for improving an effect; a stimulation feel during oral administration is reduced; and the dosage is reduced.

Description

A kind of preparation method of Lepidinm meyenii Walp extract
Technical field
The preparation method that the present invention relates to a kind of Lepidinm meyenii Walp extract, belongs to technical field of plant extraction.
Background technology
Lepidinm meyenii Walp is Cruciferae (Brassicaceae) separate row Vegetable spp (Lepidium) annual herb plant, originate in mountain areas, Andean more than Peru's height above sea level 3500 m, can be under large without fertilizer, anoxia, day and night temperature, long-term ice-bound unique environments normal growth.The nutritional labeling of Lepidinm meyenii Walp and effect aspect that can strengthen energy, endurance can match in excellence or beauty with Radix Ginseng, are therefore often called as again " Peruvian Ginseng (Peru's Radix Ginseng) ".Lepidinm meyenii Walp has long history, for thousands of years, as the Lepidinm meyenii Walp in one of important foodstuffs source of local aborigines, because it has abundant nutritive value and medicinal efficacy, especially in the function that strengthens the aspects such as energy and fertility, be considered as by people the rich gift that Andes god vouchsafes.Lepidinm meyenii Walp is larger in the cultivated area in South America, but because Lepidinm meyenii Walp has been drawn a large amount of soil nutrients in growth, under original state of cultivation, land requirement after Lepidinm meyenii Walp root is gathered several years of lying fallow could plant again, therefore, early stage to 20th century, Lepidinm meyenii Walp cultivated area reduces gradually.Lepidinm meyenii Walp is once almost endangered, is classified in the world as endangered plants.Until nineteen eighty-two, under the effort of the international organizations such as FAO (Food and Agriculture Organization of the United Nation) (FAO) and International Plant Genetic Resources Institute (IPGRI), the plant of this preciousness is just progressively promoted Jiahui common people.After this, plantation and edible Lepidinm meyenii Walp are successively repeatedly recommended again by the international organizations such as FAO to various countries, and point out that Lepidinm meyenii Walp is a kind of nutritious safe food, can solve the multiple health problem causing because of undernutrition.
The main purpose that South America locals plants Lepidinm meyenii Walp is to obtain edible, when Lepidinm meyenii Walp root using fresh herb, can fry edible together with meat or other vegetable, after drying water or milk to boil be edible, Lepidinm meyenii Walp root is squeezed into together with fruit to juice with Mel also very popular in locality as beverage, in addition Lepidinm meyenii Walp is immersed in wine and can obtains the Maca wine with tonic effect, and Lepidinm meyenii Walp leaf not only can be made Folium Camelliae sinensis, fresh leaf is because its special flavour can also be used for salad.But more Lepidinm meyenii Walp is dried as the exchange of commodities or storage (can several years of storage), make bread and cookies after can also being processed into Lepidinm meyenii Walp root powder.Because wherein fiber content is low, more crisp sweeter more eutrophy composition.Think traditionally Lepidinm meyenii Walp except being rich in nutrition, a kind of aphrodisiac especially, its energy enhancement ability, also can strengthen fertility, Lepidinm meyenii Walp is also declared can be applicable to the treatment of the diseases such as rheumatism, respiratory disorder, depression, anemia, osteoporosis, cancer, female climacteric syndrome simultaneously.The local resident of long-term edible Lepidinm meyenii Walp is in vigorous health, strong to the withstand force of disease, has according to statistics 80% local resident not spend medical station throughout the year and cures the disease.Nutritional labeling and the efficacy effects of people to Lepidinm meyenii Walp such as early stage Peru scientific researcher Chacon G carried out preliminary research, preliminary identification Lepidinm meyenii Walp improving mammal fertility, resisting fatigue, the aspects such as antidepressant have certain effect, and from wherein having found to be named as 4 kinds of active alkaloids and the glucosinolate (glucosinolates) of " macaina ", but it is since the eighties in 20th century that Lepidinm meyenii Walp obtains international botanist and medical research person's generally attention, particularly the mid-90 Italian Dini A etc. has delivered after the component analysis of the dry root of Lepidinm meyenii Walp, along with modern analysis and pharmacological effect technology mouth benefit maturation, the phytochemistry analysis of Lepidinm meyenii Walp, effect, toxicity and clinical research have had large development in late 1990s.In Lepidinm meyenii Walp, find the alkaloid of new plant chemical composition Lepidinm meyenii Walp amide (macamides) and Lepidinm meyenii Walp alkene (macaenes) and some structure uniquenesses, glucosinolate and isothiocyanic acid benzyl fat thereof are also by the analysis of system, thereby Lepidinm meyenii Walp also constantly gos deep in the efficacy study and the clinical research that improve the aspects such as fertility, sexual desire promoting.Increasing Lepidinm meyenii Walp product is as MacaPure, Vimaca, IMPERIAL GOLD MACA., SuMACATM etc. release one after another from the U.S., Peru, Kou Bendeng state, product function take strengthen energy, improve sexual function and alleviate female dimacteric syndrome as main, Lepidinm meyenii Walp completed by traditional food to " health food " and transformation.And the form of Lepidinm meyenii Walp product also by early stage MAKA dry powder, Maca wine, Lepidinm meyenii Walp beverage etc. to second filial generation Lepidinm meyenii Walp production developments such as Lepidinm meyenii Walp capsule, Lepidinm meyenii Walp tablet, Lepidinm meyenii Walp concentrated oral liquid, Lepidinm meyenii Walp complex health care products, Lepidinm meyenii Walp becomes rapidly a nova in international Medicines and Health Product market, is subject to especially extensively liking of mid-aged population, athletes, vigorous and graceful fan, female patient of climacteric syndrome of consumers in general.After beating powder with Lepidinm meyenii Walp root, conventional product great majority make Lepidinm meyenii Walp capsule, Lepidinm meyenii Walp tablet etc.
2003, Republic of Peru ambassador to China Mr. Luisv. Mr. Chang will " " MACA introduces China to natural viagra.At present, China mainly in Yunnan, the ground plantation such as Xinjiang, Tibet.In May, 2011, China's Ministry of Public Health is listed Lepidinm meyenii Walp in new resource food.
Publication number is the Chinese patent application of CN1684680A, the method of effective ingredient in a kind of water, ethanol, acetone equal solvent extraction Lepidinm meyenii Walp is disclosed, patent publication No. is the Chinese patent application of CN101756965A, the method of extracting effective ingredient in Lepidinm meyenii Walp with ethanol, methanol equal solvent is disclosed, but owing to Lepidinm meyenii Walp coarse powder not being processed, be difficult to avoid to make active substance---glucosinolate generation enzymolysis, thus reduce effect.
Summary of the invention
Think that at present the effective substance that mainly contains of Lepidinm meyenii Walp is alkaloids: alkaloid and the glucosinolate of Lepidinm meyenii Walp amide (macamides) and Lepidinm meyenii Walp alkene (macaenes) and some structure uniquenesses.For making full use of Lepidinm meyenii Walp resource, reduce the dose of Lepidinm meyenii Walp, increase curative effect, the invention provides a kind of preparation method of Lepidinm meyenii Walp extract, so that with the extract obtained Lepidinm meyenii Walp products such as Lepidinm meyenii Walp capsule, Lepidinm meyenii Walp tablet, Lepidinm meyenii Walp concentrated oral liquid, Lepidinm meyenii Walp complex health care product of making.
The present invention completes by following technical proposal: a kind of preparation method of Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) clean Lepidinm meyenii Walp Plant Powder is broken into the pueraria root powder that granularity is less than 1-3mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 50~60 ℃, and keep 15~25 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, the ethanol that the mass concentration that adds 7~9 times of amounts of pueraria root powder quality is 95%, soaks 1~3 hour, when being heated to temperature of liquid and being 70~85 ℃, reflux, extract, 2~4 hours, separates to obtain extracting solution and recrement; In a recrement, the ethanol that the mass concentration that adds 3~5 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70~85 ℃, reflux, extract, 1~3 hour, separates to obtain secondary raffinate and recrement; In secondary recrement, the ethanol that the mass concentration that adds 2~3 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70~85 ℃, reflux, extract, 1~2 hour, separates to obtain three extracting solution and recrement; Reclaim the residual ethanol in three recrements, abandon recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 10~13 times of amounts of pueraria root powder quality, stir 20~40min, quiescent setting 7~10h, isolate the clear liquid on upper strata and the precipitate of bottom, reclaim after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, press 0.5~1.5% amount of pueraria root powder quality, in concentrate, add beta-schardinger dextrin-, and stir 50~70 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.07~0.08Mpa in vacuum, temperature is, under the condition of 40~60 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
Described Lepidinm meyenii Walp plant is one or more in Lepidinm meyenii Walp root, Lepidinm meyenii Walp leaf, Lepidinm meyenii Walp branch bar.
The present invention has following advantages and effect, adopt such scheme, can carry out enzyme denaturing processing to Lepidinm meyenii Walp easily, the activity of destructive enzyme, effectively prevent the useful component in Lepidinm meyenii Walp---the hydrolysis of glucosinolate, through extracting, separating, especially at normal temperatures and pressures extract is carried out to embedding again, effectively prevented the volatilization of glucosinolate, retain this composition as far as possible, to improve effect, reduce the excitement when oral, reduce dose.
The specific embodiment
Below by embodiment, the present invention is described further.
Embodiment 1
A preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) 10 kilograms of clean Lepidinm meyenii Walp roots are ground into the pueraria root powder that granularity is less than 1mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 50 ℃, and keep 25 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, and the ethanol that the mass concentration that adds 7 times of amounts of pueraria root powder quality is 95%, soaks 3 hours, and when being heated to temperature of liquid and being 70 ℃, reflux, extract, 4 hours, separates to obtain extracting solution and recrement; In a recrement, the ethanol that the mass concentration that adds 3 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70 ℃, reflux, extract, 3 hours, separates to obtain secondary raffinate and recrement; In secondary recrement, the ethanol that the mass concentration that adds 2 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70 ℃, reflux, extract, 2 hours, separates to obtain three extracting solution and recrement; Routinely steam is passed in three recrements, distill 2 hours, to reclaim the residual ethanol in recrement, abandon recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 10 times of amounts of pueraria root powder quality, stir 20min, quiescent setting 10h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 0.5% amount of Lepidinm meyenii Walp material quantity, in concentrate, add beta-schardinger dextrin-, and stir 50 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.07Mpa in vacuum, temperature is, under the condition of 40 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
In extract, glucosinolate content is 15.6% after testing, and Lepidinm meyenii Walp alkaloid reaches 6.83%.
Embodiment 2
A preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) clean Lepidinm meyenii Walp bar is ground into the pueraria root powder that granularity is less than 3mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 60 ℃, and keep 15 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, and the ethanol that the mass concentration that adds 9 times of amounts of pueraria root powder quality is 95%, soaks 1 hour, and when being heated to temperature of liquid and being 85 ℃, reflux, extract, 2 hours, separates to obtain extracting solution and recrement; In recrement, the ethanol that the mass concentration that adds 5 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 85 ℃, reflux, extract, 3 hours, separates to obtain secondary raffinate and recrement; In recrement, the ethanol that the mass concentration that adds 3 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 85 ℃, reflux, extract, 2 hours, separates to obtain three extracting solution and recrement; Routinely steam is passed in recrement, distill 4 hours, to reclaim the residual ethanol in recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 13 times of amounts of pueraria root powder quality, stir 40min, quiescent setting 7h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 1.5% amount of material quantity (pueraria root powder), in concentrate, add beta-schardinger dextrin-, and stir 70 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.08Mpa in vacuum, temperature is, under the condition of 60 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
In extract, glucosinolate content is 9.68% after testing, and Lepidinm meyenii Walp alkaloid reaches 5.86%.
Embodiment 3
A preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) clean Lepidinm meyenii Walp leaf is ground into the pueraria root powder that granularity is less than 2mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 55 ℃, and keep 20 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, and the ethanol that the mass concentration that adds 8 times of amounts of pueraria root powder quality is 95%, soaks 2 hours, and when being heated to temperature of liquid and being 75 ℃, reflux, extract, 3 hours, separates to obtain extracting solution and recrement; In recrement, the ethanol that the mass concentration that adds 4 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 75 ℃, reflux, extract, 2 hours, separates to obtain secondary raffinate and recrement; In recrement, the ethanol that the mass concentration that adds 2 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 75 ℃, reflux, extract, 1 hour, separates to obtain three extracting solution and recrement; Routinely steam is passed in recrement, distill 3 hours, to reclaim the residual ethanol in recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 11 times of amounts of pueraria root powder quality, stir 30min, quiescent setting 8h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 1% amount of material quantity (pueraria root powder), in concentrate, add beta-schardinger dextrin-, and stir 60 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.075Mpa in vacuum, temperature is, under the condition of 50 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
In extract, glucosinolate content is 7.68% after testing, and Lepidinm meyenii Walp alkaloid reaches 6.97%.

Claims (3)

1. a preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) 10 kilograms of clean Lepidinm meyenii Walp roots are ground into the pueraria root powder that granularity is less than 1mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 50 ℃, and keep 25 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, the ethanol that the mass concentration that adds 7 times of amounts of pueraria root powder quality is 95%, soaks 3 hours, when being heated to temperature of liquid and being 70 ℃, reflux, extract, 4 hours, separates to obtain extracting solution and recrement; In a recrement, the ethanol that the mass concentration that adds 3 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70 ℃, reflux, extract, 3 hours, separates to obtain secondary raffinate and recrement; In secondary recrement, the ethanol that the mass concentration that adds 2 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 70 ℃, reflux, extract, 2 hours, separates to obtain three extracting solution and recrement; Routinely steam is passed in three recrements, distill 2 hours, to reclaim the residual ethanol in recrement, abandon recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 10 times of amounts of pueraria root powder quality, stir 20min, quiescent setting 10h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 0.5% amount of Lepidinm meyenii Walp material quantity, in concentrate, add β-cyclodextrin, and stir 50 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.07Mpa in vacuum, temperature is, under the condition of 40 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
2. a preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) clean Lepidinm meyenii Walp bar is ground into the pueraria root powder that granularity is less than 3mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 60 ℃, and keep 15 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, the ethanol that the mass concentration that adds 9 times of amounts of pueraria root powder quality is 95%, soaks 1 hour, when being heated to temperature of liquid and being 85 ℃, reflux, extract, 2 hours, separates to obtain extracting solution and recrement; In recrement, the ethanol that the mass concentration that adds 5 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 85 ℃, reflux, extract, 3 hours, separates to obtain secondary raffinate and recrement; In recrement, the ethanol that the mass concentration that adds 3 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 85 ℃, reflux, extract, 2 hours, separates to obtain three extracting solution and recrement; Routinely steam is passed in recrement, distill 4 hours, to reclaim the residual ethanol in recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 13 times of amounts of pueraria root powder quality, stir 40min, quiescent setting 7h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 1.5% amount of raw material pueraria root powder amount, in concentrate, add β-cyclodextrin, and stir 70 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.08Mpa in vacuum, temperature is, under the condition of 60 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
3. a preparation method for Lepidinm meyenii Walp extract, is characterized in that through the following step:
1) clean Lepidinm meyenii Walp leaf is ground into the pueraria root powder that granularity is less than 2mm;
2) pueraria root powder of step 1) is put into microwave dryer, heat up and be heated to 55 ℃, and keep 20 minutes, be cooled to room temperature, to carry out enzyme denaturing processing, the activity of destructive enzyme, prevents glucosinolate hydrolysis;
3) by step 2) pueraria root powder of enzyme denaturing processing puts into extraction pot, the ethanol that the mass concentration that adds 8 times of amounts of pueraria root powder quality is 95%, soaks 2 hours, when being heated to temperature of liquid and being 75 ℃, reflux, extract, 3 hours, separates to obtain extracting solution and recrement; In recrement, the ethanol that the mass concentration that adds 4 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 75 ℃, reflux, extract, 2 hours, separates to obtain secondary raffinate and recrement; In recrement, the ethanol that the mass concentration that adds 2 times of amounts of pueraria root powder quality is 95%, when being heated to temperature of liquid and being 75 ℃, reflux, extract, 1 hour, separates to obtain three extracting solution and recrement; Routinely steam is passed in recrement, distill 3 hours, to reclaim the residual ethanol in recrement;
4) combining step 3) one, two, three extracting solution, in extracting solution, add the water of 11 times of amounts of pueraria root powder quality, stir 30min, quiescent setting 8h, isolate the ethanol clear liquid on upper strata and the precipitate of bottom, reclaim routinely after the ethanol in precipitate, abandon residue;
5) ethanol in the clear liquid of concentration and recovery step 4), being concentrated into concentrate proportion is 1.05, by 1% amount of raw material pueraria root powder amount, in concentrate, add β-cyclodextrin, and stir 60 minutes, extract is carried out to embedding, prevent the volatilization of glucosinolate, reduce the excitement when oral;
6) by the embedding extract of step 5), be 0.075Mpa in vacuum, temperature is, under the condition of 50 ℃, to carry out vacuum drying, until the moisture of embedding extract, below 5%, is pulverized through routine, sieves, and obtains Lepidinm meyenii Walp extract.
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1473594A (en) * 2003-07-01 2004-02-11 华中科技大学 Extract of Maka root

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1473594A (en) * 2003-07-01 2004-02-11 华中科技大学 Extract of Maka root

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
浦跃武等.玛咖多糖的抗氧化性能研究.《安徽农业科学》.2009,第37卷(第28期),13803-13805页. *
甘瑾等.三种色型玛咖芥子油苷组分及含量分析.《中国农业科学》.2012,第45卷(第7期),1365-1371页. *

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