CN103007343B - Hard tissue repair and substitute material and preparation method thereof - Google Patents

Hard tissue repair and substitute material and preparation method thereof Download PDF

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CN103007343B
CN103007343B CN 201310015917 CN201310015917A CN103007343B CN 103007343 B CN103007343 B CN 103007343B CN 201310015917 CN201310015917 CN 201310015917 CN 201310015917 A CN201310015917 A CN 201310015917A CN 103007343 B CN103007343 B CN 103007343B
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tissue repair
hard tissue
calcium phosphate
replacement materials
biphasic calcium
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CN 201310015917
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CN103007343A (en
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蒋电明
谯波
李鸿
严永刚
曹侠
郝新彦
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重庆医科大学
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Abstract

本发明属于生物材料技术领域,提供了一种硬组织修复及替代材料及其制备方法,该硬组织修复及替代材料由添加了聚氨基酸(PAA)的双相钙磷陶瓷(BCP)构成。 The present invention belongs to the technical field of biological materials, there is provided a hard tissue repair and replacement materials and preparation method, the hard tissue repair and replacement materials composed of a polyamino acid is added (PAA) biphasic calcium phosphate ceramic (BCP). 通过添加聚氨基酸改良双相钙磷陶瓷的力学强度,获得了兼具良好生物学活性和足够力学强度的硬组织修复及替代材料,解决了单一双相钙磷陶瓷的力学强度较低,难以作为骨修复和替代材料起到结构性支撑的作用而临床应用受到极大制约的问题,该制备方法简单,成本较低,实用性强,具有较强的推广与应用价值。 By adding mechanical strength polyamino acid modified biphasic calcium phosphate ceramics, to obtain hard tissue repair and replacement materials combine good biological activity and a sufficient mechanical strength to solve the low mechanical strength of the single biphasic calcium phosphate ceramic, a hard repair and bone substitute materials play a supporting role in structural issues and clinical application is greatly restricted, and the preparation method is simple, low cost, practical, with strong promotion and application value.

Description

一种硬组织修复及替代材料及其制备方法 A hard tissue repair and replacement materials and preparation method

技术领域 FIELD

[0001] 本发明属于生物材料技术领域,尤其涉及一种硬组织修复及替代材料及其制备方法。 [0001] The present invention belongs to the technical field of biological materials, particularly to a hard tissue repair and replacement materials and preparation method.

背景技术 Background technique

[0002]目前硬组织修复和替代的生物材料品种繁多,但能成功运用于临床的很少,主要原因是不能使生物材料的生物活性和力学强度达到很好的平衡,特别是在力学强度上往往达不到临床应用的要求。 [0002] Currently hard tissue repair and replacement of a wide variety of biological material, but rarely used in clinical success, the main reason is not the biological activity and mechanical strength of biological material to achieve a good balance, especially in the mechanical strength often fail to clinical applications. 双相钙磷陶瓷(biphasic calcium phosphate, BCP)是由羟基磷灰石和β -磷酸三钙组成的生物活性材料,结合了羟基磷灰石(hydroxyapatite, HA)的高强度、高生物活性、稳定性以及β_磷酸三钙(β -tricalcium phosphate, β -TCP)的吸收降解特性,BCP作为骨修复材料长期以来得到了大量深入研究和广泛应用。 Biphasic calcium phosphate ceramic (biphasic calcium phosphate, BCP) is composed of hydroxyapatite and β - tricalcium phosphate bioactive material composed of a combination of hydroxyapatite (hydroxyapatite, HA) is a high strength, high biological activity, stability as well as β_ tricalcium phosphate (β -tricalcium phosphate, β -TCP) absorption degradation characteristics, BCP as a bone repair material has long been widely used and a large number of in-depth study. BCP具有优良的骨诱导性和骨传导性,能加工成多孔结构,并能通过多孔结构调节材料的降解性能和力学强度。 BCP has excellent osteoinductive and osteoconductive, it can be processed into a porous structure, and by adjusting the porosity and degradation of the mechanical strength of the material. 但单一的双相钙磷陶瓷(BCP)的力学强度较低,仅0.5~5MPa,难以作为骨修复和替代材料起到结构性支撑的作用,使得其临床应用受到极大制约。 But lower single biphasic calcium phosphate ceramic (BCP) mechanical strength, only 0.5 ~ 5MPa, it is difficult to repair and as a bone substitute material plays the role of structural support, so that its clinical application is greatly restricted.

发明内容 SUMMARY

[0003] 本发明提供了一种硬组织修复及替代材料及其制备方法,旨在解决单一双相钙磷陶瓷的力学强度较低,难以作为骨修复和替代材料起到结构性支撑的作用而临床应用受到极大制约的问题。 [0003] The present invention provides a hard tissue repair and replacement materials preparation, to solve the low mechanical strength of the single biphasic calcium phosphate ceramic, it is difficult to play a role as a structural support to a bone repair and replacement materials and clinical application is greatly restricted in question.

[0004] 本发明的目的在于提供一种硬组织修复及替代材料,该硬组织修复及替代材料由添加了聚氨基酸(PAA)的双相钙磷陶瓷(BCP)构成。 [0004] The object of the present invention is to provide a hard tissue repair and replacement materials, tissue repair and replace the hard material consists of poly amino acid is added (PAA) biphasic calcium phosphate ceramic (BCP).

[0005] 本发明的另一目的在于提供一种硬组织修复及替代材料的制备方法,该制备方法包括以下步骤: [0005] Another object of the present invention is to provide a preparation method for hard tissue repair and replacement materials, the preparation process comprises the steps of:

[0006] 步骤一,将63.16g 6-氨基己酸、2.76g脯氨酸、2.68g丙氨酸、2.96g苯丙氨酸、 [0006] Step a, to 63.16g 6- aminocaproic acid, 2.76 g of proline, 2.68 g of alanine, 2.96 g of phenylalanine,

1.8g赖氨酸、3.0Og羟脯氨酸和100mL水加入到250ml带有搅拌装置和氮气保护装置的三颈瓶中; 1.8g lysine, 3.0Og hydroxyproline and 100mL of water were added to the 250ml three-necked flask equipped with a stirrer and nitrogen protection device;

[0007] 步骤二,开启氮气保护和搅拌,逐步升温至200°C,脱水完成后升温至210°C,在此温度下将反应物全部熔融成亮黄透明的熔体; [0007] Step two, open and nitrogen with stirring, and gradually heated to 200 ° C, after the completion of dehydration warmed to 210 ° C, at this temperature the reaction was complete fusion into the light yellow transparent melt;

[0008] 步骤三,待单体熔融后升温至220°C开始聚合反应3小时,并加入49.89g含量为40%的双相磷酸钙陶瓷,升温至230°C反应3小时,反应结束后在氮气保护下冷却到室温得到块状硬组织修复及替代材料。 [0008] Step three, until the monomers melted warmed to 220 ° C to start the polymerization reaction for 3 hours, was added 49.89g content of 40% biphasic calcium phosphate ceramics, warmed to 230 ° C for 3 hours. After completion of the reaction under nitrogen was cooled to room temperature to obtain a massive hard tissue repair and replacement materials.

[0009] 本发明提供的硬组织修复及替代材料及其制备方法,通过添加聚氨基酸改良双相钙磷陶瓷的力学强度,获得了兼具良好生物学活性和足够力学强度的硬组织修复及替代材料,解决了单一双相钙磷陶瓷的力学强度较低,难以作为骨修复和替代材料起到结构性支撑的作用而临床应用受到极大制约的问题,该制备方法简单,成本较低,实用性强,具有较强的推广与应用价值。 [0009] The hard tissue repair and replacement materials according to the present invention provides a preparation method, the mechanical strength by addition of acid modified polyethylene biphasic calcium phosphate ceramics, to obtain both good biological activity and a sufficient mechanical strength of the hard tissue repair and substitute materials, to solve the low mechanical strength of the single biphasic calcium phosphate ceramic, it is difficult as the role of bone repair and replacement materials play a structural support and issue clinical application is greatly restricted, and the preparation method is simple, low cost, practical and strong, with a strong promotion and application value. 附图说明 BRIEF DESCRIPTION

[0010] 图1是本发明实施例提供的硬组织修复及替代材料的制备方法的实现流程图。 [0010] FIG. 1 is a flow chart for implementing hard tissue repair and replacement materials prepared according to an embodiment of the present invention. 具体实施方式 Detailed ways

[0011] 为了使本发明的目的、技术方案及优点更加清楚明白,以下结合附图及实施例,对本发明进行进一步的详细说明。 [0011] To make the objectives, technical solutions and advantages of the present invention will become more apparent hereinafter in conjunction with the accompanying drawings and embodiments of the present invention will be described in further detail. 应当理解,此处所描述的具体实施例仅仅用以解释本发明,并不用于限定发明。 It should be understood that the specific embodiments described herein are only intended to illustrate the present invention, not to limit the invention.

[0012] 本发明的目的在于提供一种硬组织修复及替代材料,该硬组织修复及替代材料由添加了聚氨基酸(PAA)的双相钙磷陶瓷(BCP)构成。 [0012] The object of the present invention is to provide a hard tissue repair and replacement materials, tissue repair and replace the hard material consists of poly amino acid is added (PAA) biphasic calcium phosphate ceramic (BCP).

[0013] 图1示出了本发明实施例提供的硬组织修复及替代材料的制备方法的实现流程。 [0013] FIG 1 illustrates a process of the present invention is to realize the hard tissue repair and replacement materials prepared according to an embodiment.

[0014] 该制备方法包括以下步骤: [0014] The preparation method comprises the steps of:

[0015] 在步骤SlOl中,将63.16g 6_氨基己酸、2.76g脯氨酸、2.68g丙氨酸、2.96g苯丙氨酸、1.8g赖氨酸、3.0Og羟脯氨酸和100mL水加入到250ml带有搅拌装置和氮气保护装置的三颈瓶中; [0015] In step SlOl, the 63.16g 6_-aminocaproic acid, 2.76 g of proline, 2.68 g of alanine, 2.96 g of phenylalanine, 1.8 g of lysine, 3.0Og hydroxyproline and 100mL water was added to a 250ml three-necked flask equipped with a stirrer and nitrogen protection device;

[0016] 在步骤S102中,开启氮气保护和搅拌,逐步升温至200°C,脱水完成后升温至210°C,在此温度下将反应物全部熔融成亮黄透明的熔体; [0016] In step S102, opening and nitrogen with stirring, and gradually heated to 200 ° C, after the completion of dehydration warmed to 210 ° C, at this temperature the reaction was complete fusion into the light yellow transparent melt;

[0017] 在步骤S103中,待单体熔融后升温至220°C开始聚合反应3小时,并加入49.89g含量为40%的双相磷酸钙陶瓷,升温至230°C反应3小时,反应结束后在氮气保护下冷却到室温得到块状硬组织修复及替代材料。 [0017] In step S103, until the monomers melted warmed to 220 ° C to start the polymerization reaction for 3 hours, was added 49.89g content of 40% biphasic calcium phosphate ceramics, warmed to 230 ° C for 3 hours, the reaction after cooling to room temperature to obtain a massive hard tissue repair and replacement materials under nitrogen.

[0018] 下面结合附图及具体实施例对本发明的应用原理作进一步描述。 [0018] Specific embodiments of the application of the principles of the present invention will be further described in conjunction with the accompanying drawings and the following.

[0019] 本发明拟通过添加聚氨基酸(PAA)改良双相钙磷陶瓷的力学强度,使其成为兼具良好生物学活性和足够力学强度的硬组织修复和替代材料 [0019] The present invention is intended by the addition of mechanical strength polyamino acids (PAA) biphasic calcium phosphate ceramic is improved, making it hard tissue repair and replacement material both good biological activity and a sufficient mechanical strength

[0020] 图1示出了本发明实施例提供的硬组织修复及替代材料的制备方法的实现流程,将63.16g 6-氨基己酸,2.76g脯氨酸,2.68g丙氨酸,2.96g苯丙氨酸,1.8g赖氨酸,3.0Og羟脯氨酸和100mL水加入到250ml带有搅拌装置和氮气保护装置的三颈瓶中,开启氮气保护和搅拌,逐步升温至200 °C,脱水完成后升温至210 °C,此温度下反应物可全部熔融,成为亮黄透明的熔体。 [0020] FIG 1 illustrates a process of the present invention is to realize the hard tissue repair and replacement materials prepared according to an embodiment will 63.16g 6- aminocaproic acid, 2.76 g of proline, 2.68 g of alanine, 2.96 g of phenylalanine, 1.8 g of lysine, 3.0Og hydroxyproline and 100mL of water were added to the 250ml three-necked flask equipped with a stirrer and nitrogen protection devices, opening and nitrogen with stirring, and gradually heated to 200 ° C, after completion of dehydration temperature was raised to 210 ° C, this temperature of reactants can be melted, and the melt became bright yellow and transparent. 待单体熔融后升温至220 °C开始聚合反应3h。 After the monomer was heated to 220 ° C melt polymerization was started 3h. 此时,加入49.89g含量约为40 %的双相磷酸钙陶瓷,升温至230 °C反应3h,反应结束后在氮气保护下冷却到室温得到块状复合材料。 At this time, was added 49.89g content of about 40% biphasic calcium phosphate ceramics, the reaction temperature was raised to 230 ° C 3h, after the reaction was cooled to room temperature under a nitrogen atmosphere to obtain a composite bulk material.

[0021] 本发明实施例提供的硬组织修复及替代材料及其制备方法,通过添加聚氨基酸改良双相钙磷陶瓷的力学强度,获得了兼具良好生物学活性和足够力学强度的硬组织修复及替代材料,解决了单一双相钙磷陶瓷的力学强度较低,难以作为骨修复和替代材料起到结构性支撑的作用而临床应用受到极大制约的问题,该制备方法简单,成本较低,实用性强,具有较强的推广与应用价值。 [0021] Alternatively and hard tissue repair material and its preparation method provided in the embodiment of the present invention, the mechanical strength by adding a polyamino acid modified biphasic calcium phosphate ceramics, to obtain both good biological activity and a sufficient mechanical strength of the hard tissue repair and the role of alternative materials to solve the low mechanical strength of the single biphasic calcium phosphate ceramic, it is difficult to play as a structural support bone repair and replacement materials and issue clinical application is greatly restricted, and the preparation method is simple, low cost , practical, with strong promotion and application value.

[0022] 以上所述仅为本发明的较佳实施例而已,并不用以限制本发明,凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等,均应包含在本发明的保护范围之内。 [0022] The foregoing is only preferred embodiments of the present invention but are not intended to limit the present invention, any modifications within the spirit and principle of the present invention, equivalent substitutions and improvements should be included in the present within the scope of the invention.

Claims (1)

1.一种硬组织修复及替代材料的制备方法,其特征在于,该硬组织修复及替代材料由添加了聚氨基酸(PAA)的双相钙磷陶瓷(BCP)构成; 该硬组织修复及替代材料的制备方法包括以下步骤: 步骤一,将63.16g6-氨基己酸、2.76g脯氨酸、2.68g丙氨酸、2.96g苯丙氨酸、1.8g赖氨酸、3.0Og羟脯氨酸和100mL水加入到250ml带有搅拌装置和氮气保护装置的三颈瓶中;步骤二,开启氮气保护和搅拌,逐步升温至200°C,脱水完成后升温至210°C,在此温度下将反应物全部熔融成亮黄透明的熔体; 步骤三,待单体熔融后升温至220°C开始聚合反应3小时,并加入49.89g含量为40%的双相磷酸钙陶瓷,升温至230°C反应3小时,反应结束后在氮气保护下冷却到室温得到块状硬组织修复及替代材料。 CLAIMS 1. A method for preparing hard tissue repair and replacement materials, wherein the hard tissue repair and replacement materials composed of a polyamino acid is added (PAA) biphasic calcium phosphate ceramics (the BCP); hard tissue repair and replace the the method of preparing a material comprising the following steps: step one, the 63.16g6- aminocaproic acid, 2.76 g of proline, 2.68 g of alanine, 2.96 g of phenylalanine, 1.8 g of lysine, 3.0Og hydroxyproline and 100mL of water were added to the 250ml three-necked flask equipped with a stirrer and nitrogen protection device; two step, open the nitrogen and stirring, and gradually heated to 200 ° C, after the completion of dehydration warmed to 210 ° C, at this temperature the reaction was complete fusion into the light yellow transparent melt; step three, until the monomers melted warmed to 220 ° C to start the polymerization reaction for 3 hours, was added 49.89g content of 40% biphasic calcium phosphate ceramics, warmed to 230 ° C for 3 hours. after the reaction was cooled to room temperature under a nitrogen atmosphere to obtain a massive hard tissue repair and replacement materials. ` `
CN 201310015917 2013-01-16 2013-01-16 Hard tissue repair and substitute material and preparation method thereof CN103007343B (en)

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US20080102097A1 (en) 2006-10-31 2008-05-01 Zanella John M Device and method for treating osteolysis using a drug depot to deliver an anti-inflammatory agent
CN101342384A (en) 2008-08-25 2009-01-14 四川国纳科技有限公司 Composite polymer bone-renovation material containing ceramic component and preparation method thereof

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Publication number Priority date Publication date Assignee Title
US20080102097A1 (en) 2006-10-31 2008-05-01 Zanella John M Device and method for treating osteolysis using a drug depot to deliver an anti-inflammatory agent
CN101342384A (en) 2008-08-25 2009-01-14 四川国纳科技有限公司 Composite polymer bone-renovation material containing ceramic component and preparation method thereof

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