CN102972277A - Method for quickly raising clonal seedlings of teakwood by light matrix tray - Google Patents
Method for quickly raising clonal seedlings of teakwood by light matrix tray Download PDFInfo
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Abstract
本发明公开了一种柚木无性系轻基质穴盘快速育苗的方法。该育苗方法以柚木无根组培瓶苗为繁殖材料、选取育苗轻型基质和用育苗穴盘代替育苗袋进行快速培植,实现了轻基质+育苗穴盘的快速工厂化育苗,节约了育苗和造林成本,提高了育苗效率和造林效率,降低了运输成本和造林工人的劳动强度。本发明的柚木无根幼苗在移栽后7天即开始生根,与现有的用营养土袋的柚木育苗技术相比,生根时间提前了3天,生根时间集中,生根成活率从87.6%提高到95.3%。本发明采用柚木无根组培瓶苗直接进行移栽繁殖系数高,保持了柚木优良无性系的遗传特性,育苗时间较短,移栽3~4个月可出圃造林,可快速为市场提供大量的良种壮苗。
The invention discloses a method for rapidly growing seedlings in light-substrate plugs of teak clones. The seedling raising method uses teak rootless tissue culture bottle seedlings as propagation materials, selects a light substrate for seedling cultivation, and replaces seedling cultivation bags with seedling trays for rapid cultivation, realizes rapid industrial seedling cultivation of light substrate + seedling trays, and saves seedling cultivation and afforestation It improves the efficiency of seedling raising and afforestation, and reduces the transportation cost and labor intensity of afforestation workers. The teak rootless seedlings of the present invention begin to take root 7 days after transplanting, compared with the existing technology of teak seedling cultivation with nutrient soil bags, the rooting time is advanced by 3 days, the rooting time is concentrated, and the rooting survival rate is improved from 87.6% to 95.3%. The present invention adopts teak rootless tissue culture bottle seedlings to be directly transplanted and has a high reproduction coefficient, maintains the genetic characteristics of excellent teak clones, has a short seedling time, can be out of the nursery for afforestation within 3 to 4 months after transplanting, and can quickly provide a large amount of teak for the market Strong seedlings of fine varieties.
Description
技术领域technical field
本发明属于乔木的培植繁殖技术领域,特别涉及一种柚木无性系轻基质穴盘快速育苗的方法。The invention belongs to the technical field of arbor cultivation and propagation, and in particular relates to a method for rapidly growing seedlings in light-substrate plugs of teak clones.
背景技术Background technique
柚木(Tectona grandis L.f.)为马鞭草科(Verbenaceae)柚木属(Tectona)高大乔木,天然分布于北纬9°~26°,东经73°~104°的印度南部与中部、泰国北部、缅甸和老挝,是著名的热带珍贵用材树种,素有“万木之王”的美誉,其心材材质坚韧耐久,结构致密,纹理美观,是制造军舰、码头、桥梁、建筑、家具、雕刻和木地板等的高级用材。柚木具有生长快,材质优良,用途广和投资回报率高等特点,在热带、南亚热带地区广为种植,是世界上人工林种植面积最大的4个树种之一,也是单位面积产值最高的造林树种。Teak (Tectona grandis L.f.) is a tall tree of the genus Tectona in the family Verbenaceae. It is naturally distributed in southern and central India, northern Thailand, Myanmar and Laos at 9°-26° north latitude and 73°-104° east longitude. It is a famous tropical precious timber tree species, known as the "King of Ten Thousand Woods". Its heartwood is tough and durable, with a dense structure and beautiful texture. Timber. Teak has the characteristics of fast growth, excellent material, wide application and high return on investment. It is widely planted in tropical and subtropical regions. It is one of the four tree species with the largest plantation area in the world, and it is also the afforestation tree species with the highest output value per unit area. .
我国引进柚木栽培已有180多年历史,通过对柚木培育技术的研究,现在推广种植范围遍及10个省(自治区)60多个县(市),总面积约1.5万hm2。现在,云南省、海南省把柚木列为主要造林树种,广东、广西、福建、贵州省采用柚木造林的私人与企业也越来越多。然而,目前我国柚木商品林的发展以种子实生苗和优良组培无性系苗为主的苗木培育常以黄心土为基质的营养袋育苗,依靠手工装袋,育苗费工效率低,而且每株重0.50~0.75㎏,大面积造林时运输成本高,尤其是在我国热带南亚热带山区造林,因营养袋苗重,工人挑苗上山困难,且量少,效率低。而将小苗培育成截干苗,则常需8~12个月,且起苗需修剪主根、侧根和须根,费工成本高,苗木造林后萌芽慢,根系恢复时间长,造成生长期短,生长慢,耐寒和抗风能力降低。因此,培育出根系完整又轻便的苗木对山地柚木造林尤为重要。China has introduced teak cultivation for more than 180 years. Through the research on teak cultivation technology, the promotion and planting scope covers more than 60 counties (cities) in 10 provinces (autonomous regions), with a total area of about 15,000 hm 2 . Now, Yunnan and Hainan provinces list teak as the main tree species for afforestation, and more and more private individuals and enterprises use teak for afforestation in Guangdong, Guangxi, Fujian, and Guizhou provinces. However, at present, the development of teak commercial forests in my country is mainly based on seed seedlings and excellent tissue culture clone seedlings. Nutrient bag seedlings are often used as the matrix of yellow core soil, relying on manual bagging, and the labor efficiency of seedling cultivation is low, and each plant weighs 0.50~0.75kg, the transportation cost is high when large-scale afforestation, especially in the afforestation of tropical and subtropical mountainous areas in my country, due to the weight of seedlings in nutrition bags, it is difficult for workers to pick seedlings up the mountain, and the quantity is small and the efficiency is low. However, it usually takes 8 to 12 months to cultivate young seedlings into cut dry seedlings, and the main roots, lateral roots and fibrous roots need to be pruned to raise the seedlings. Slow growth, reduced cold and wind resistance. Therefore, cultivating seedlings with complete and light root system is particularly important to mountain teak afforestation.
发明内容Contents of the invention
本发明的目的在于克服现有技术的缺点与不足,提供一种柚木无性系轻基质穴盘快速育苗的方法。该育苗方法以柚木无根组培瓶苗为繁殖材料、选取育苗轻型基质(轻基质)和用育苗穴盘代替育苗袋进行快速育苗培植,节约了育苗和造林成本,提高了育苗效率和造林效率,培育的轻基质苗每株仅重0.15~0.20㎏,降低了运输成本和造林工人的劳动强度。The purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art, and provide a method for rapidly growing seedlings in light-substrate plugs of teak clones. The seedling raising method uses teak rootless tissue culture bottle seedlings as propagation materials, selects light substrate for seedling cultivation (light substrate) and replaces seedling cultivation bags with seedling cultivation trays for rapid seedling cultivation, which saves the cost of seedling cultivation and afforestation, and improves the efficiency of seedling cultivation and afforestation , The weight of each cultivated light-substrate seedling is only 0.15-0.20 kg, which reduces the transportation cost and labor intensity of afforestation workers.
本发明的目的通过下述技术方案实现:一种柚木无性系轻基质穴盘快速育苗的方法,包括以下步骤:The object of the present invention is achieved through the following technical solutions: a method for rapid seedling cultivation of teak clone light matrix plug, comprising the following steps:
(1)繁殖材料的准备(1) Preparation of propagation materials
将柚木无性系组培瓶苗移到荫棚下练苗10~15天后,剪去基部的愈伤组织,得到幼苗,移栽前将幼苗浸泡于清水中;Move the teak clone tissue culture bottle seedlings to the shade for 10-15 days, cut off the callus at the base to obtain seedlings, soak the seedlings in clear water before transplanting;
(2)育苗轻基质装盘与消毒(2) Packing and disinfection of light substrate for seedling cultivation
将育苗轻基质装满育苗盘后置于铁架上,在移栽前0.5~1天用高猛酸钾水溶液浇透消毒,盖上薄膜备用;Fill the seedling raising tray with the light substrate for seedling cultivation and place it on the iron frame. Water and disinfect with potassium permanganate aqueous solution 0.5 to 1 day before transplanting, and cover with a film for later use;
(3)移栽(3) Transplanting
将步骤(1)中浸泡于清水中的幼苗移栽至步骤(2)中消毒过的育苗轻基质育苗盘中,移栽完后喷清水定根,喷雾消毒后盖上薄膜保湿,得到保湿的幼苗;Transplant the seedlings soaked in clean water in step (1) to the light substrate seedling tray sterilized in step (2), spray clean water to fix the roots after transplanting, cover with a film to moisturize after spray disinfection, and obtain moisturizing seedling;
(4)后期管理(4) Later management
于移栽后第3~4天对步骤(3)中保湿的幼苗进行第二次消毒,之后每周消毒1~2次,移栽20~25天后浇施尿素或复合肥水溶液,苗高15~20cm时可出圃造林;On the 3rd to 4th day after transplanting, sterilize the seedlings kept moist in step (3) for the second time, and then sterilize 1 to 2 times a week, and pour urea or compound fertilizer aqueous solution after 20 to 25 days of transplanting. The height of the seedlings is 15 ~20cm can be out of the nursery for afforestation;
步骤(1)~(4)的操作优选在阴棚中进行;所述的荫棚的条件优选为遮荫度70~90%、湿度80~100%和温度25~28℃;The operations of steps (1) to (4) are preferably carried out in an awning; the conditions of the awning are preferably shaded at 70-90%, humidity at 80-100%, and temperature at 25-28°C;
步骤(1)中:In step (1):
所述的柚木无性系组培瓶苗含有2~4对叶,高5~10cm;柚木无性系组培瓶苗的基部为愈伤组织、没有根系,不需经过生根培养;The teak clone tissue culture bottle seedling contains 2 to 4 pairs of leaves, and the height is 5 to 10 cm; the base of the teak clone tissue culture bottle seedling is callus tissue, no root system, and does not need to undergo rooting culture;
所述的浸泡的时间优选为0~3h;The soaking time is preferably 0 to 3 hours;
步骤(2)中:In step (2):
所述的铁架的高度优选为15~20cm;The height of the iron frame is preferably 15-20cm;
所述的育苗轻基质采用以下方法进行配制:将泥炭土、蛭石、珍珠岩、椰糠和黄心土按体积比1~1.5:1~1.5:0.5~1:2:2混合均匀,得到育苗轻基质;The light substrate for seedling cultivation is prepared by the following method: peat soil, vermiculite, perlite, coir peat and yellow core soil are mixed uniformly in a volume ratio of 1-1.5:1-1.5:0.5-1:2:2 to obtain a light substrate for seedling cultivation. matrix;
所述的育苗轻基质优选采用以下方法进行配制:将泥炭土、蛭石、珍珠岩、椰糠和黄心土按体积比1:1:1:2:2混合均匀,得到育苗轻基质;The light substrate for seedling cultivation is preferably prepared by the following method: uniformly mix peat soil, vermiculite, perlite, coir peat and yellow core soil in a volume ratio of 1:1:1:2:2 to obtain a light substrate for seedling cultivation;
所述的育苗盘优选为长宽高540mm×280mm×110mm的育苗盘,更优选为50孔的塑料育苗盘,每盘培育50株苗;The seedling tray is preferably a seedling tray with a length, width, and height of 540mm×280mm×110mm, more preferably a plastic seedling tray with 50 holes, and 50 seedlings are cultivated in each tray;
所述的育苗盘的孔为方形或圆形,上大下小;The hole of the seedling tray is square or circular, the upper part is larger and the lower part is smaller;
所述的方形孔的上部边长为46mm,下部为直径10mm的圆形排水孔;The upper part of the square hole has a side length of 46 mm, and the lower part is a circular drainage hole with a diameter of 10 mm;
所述的高猛酸钾水溶液优选为3wt‰的高猛酸钾水溶液;Described potassium permanganate aqueous solution is preferably the potassium permanganate aqueous solution of 3wt‰;
步骤(3)中:In step (3):
所述的移栽的时间为阴天或晴天,雨天不能移栽;阴天可全天移栽;晴天11点30~15点太阳光照强时不宜移栽;The time for transplanting is cloudy or sunny, rainy days cannot be transplanted; cloudy days can be transplanted all day; sunny days are not suitable for transplanting when the sun is strong from 11:30 to 15:00;
所述的移栽优选采用以下方法进行移栽:先用竹签或小木棒在育苗盘每孔的育苗轻基质中央插1小孔,将幼苗移栽入小孔中,再用竹签或小木棒将幼苗一侧压实;移栽过程中若温度高,可用喷雾器喷水防止幼苗萎蔫;The described transplanting preferably adopts the following method to transplant: first use a bamboo stick or a small wooden stick to insert a small hole in the center of the seedling light substrate in each hole of the seedling tray, transplant the seedlings into the small hole, and then use a bamboo stick or a small wooden stick Compact one side of the seedlings with sticks; if the temperature is high during transplanting, spray water with a sprayer to prevent the seedlings from wilting;
所述的小孔的深度优选为1.5~2.5cm;The depth of the small hole is preferably 1.5-2.5 cm;
所述的喷雾消毒优选采用1/800的百菌清水溶液进行喷雾消毒;The spray disinfection preferably adopts 1/800 chlorothalonil aqueous solution for spray disinfection;
所述的1/800的百菌清水溶液是指百菌清和水按质量比1:799混合得到的溶液;The 1/800 chlorothalonil aqueous solution refers to a solution obtained by mixing chlorothalonil and water at a mass ratio of 1:799;
步骤(4)中:In step (4):
所述的消毒优选采用1/800甲基托布津水溶液、1/800百菌清水溶液或1/800多菌灵水溶液交替进行消毒;The disinfection is preferably performed alternately with 1/800 thiophanate-methyl aqueous solution, 1/800 chlorothalonil aqueous solution or 1/800 carbendazim aqueous solution;
所述的1/800的甲基托布津水溶液是指甲基托布津和水按质量比1:799混合得到的溶液;The 1/800 thiophanate-methyl aqueous solution refers to a solution obtained by mixing thiophanate-methyl and water in a mass ratio of 1:799;
所述的1/800的多菌灵水溶液是指多菌灵和水按质量比1:799混合得到的溶液;The 1/800 carbendazim aqueous solution refers to a solution obtained by mixing carbendazim and water at a mass ratio of 1:799;
所述的尿素水溶液为1~1.5wt‰的尿素水溶液,优选为1wt‰的尿素水溶液;The urea aqueous solution is 1-1.5wt‰ urea aqueous solution, preferably 1wt‰ urea aqueous solution;
所述的复合肥水溶液为1~1.5wt‰的复合肥水溶液,优选为1wt‰的复合肥水溶液;The compound fertilizer aqueous solution is a compound fertilizer aqueous solution of 1 to 1.5wt‰, preferably a compound fertilizer aqueous solution of 1wt‰;
所述的复合肥优选为含有15wt%N、15wt%P和15wt%K的复合肥;Described compound fertilizer is preferably the compound fertilizer containing 15wt%N, 15wt%P and 15wt%K;
移栽后,晴天每天需要喷水2~3次,分别为上午9点、下午6点,另1次可根据情况定,以保持幼苗叶片湿润不萎蔫为准;太阳光照强、温度高于30℃时,需要在薄膜上加盖遮阳网,10~17点每隔1小时膜外喷水1次,阴天可于下午5点喷1次或根据苗情不喷,雨天不喷;After transplanting, spray water 2 to 3 times a day on sunny days, at 9 am and 6 pm, and the other time can be determined according to the situation, so as to keep the leaves of the seedlings moist and not wilting; the sun is strong and the temperature is higher than 30 ℃. When the temperature is ℃, it is necessary to cover the film with a sunshade net, spray water once every hour from 10 to 17 o'clock, and spray once at 5 o'clock in the afternoon on cloudy days or not spray according to the condition of the seedlings, and not spray on rainy days;
移栽1.5个月后,消毒的浓度可增加到3/800~5/800,施肥的浓度可增加到3~5wt‰,此时晴天或阴天可揭开薄膜练苗,雨天也可以打开薄膜两端通气;After 1.5 months of transplanting, the concentration of disinfection can be increased to 3/800-5/800, and the concentration of fertilization can be increased to 3-5wt‰. At this time, the film can be uncovered to practice seedlings on sunny or cloudy days, and the film can also be opened on rainy days ventilation at both ends;
所述移栽操作的时间,广州优选在每年的4月下旬至7月中旬,最优为5~6月,海南优选在每年的3月中旬至11月中旬;The time for the transplanting operation is preferably from late April to mid-July in Guangzhou, preferably from May to June, and in mid-March to mid-November in Hainan;
移栽1周后幼苗开始生根,此时幼苗已进入稳定期,2周后大量生根或苗木发根齐全,此时认为移栽成活,之后根系伸长,侧根增多,逐渐形成成熟完整的根系;移栽3~4个月后苗高达15~20cm,可出圃造林;1 week after transplanting, the seedlings began to take root. At this time, the seedlings had entered a stable period. After 2 weeks, a large number of roots took root or the seedlings had fully developed roots. At this time, the transplanting was considered to be alive. Afterwards, the root system elongated, the lateral roots increased, and gradually formed a mature and complete root system; After 3-4 months of transplanting, the seedlings are 15-20cm high and can be planted out of the nursery;
本发明的原理:本发明利用植物枝插生根原理,植物枝扦插后在枝条内的形成层和维管束鞘组织形成根原始体,从而发育长出不定根,并逐渐形成根系,属于皮部生根类型的育苗方式。The principle of the present invention: the present invention utilizes the rooting principle of plant branch cuttings. After plant branch cuttings, the cambium and vascular bundle sheath tissue in the branches form a root primitive, thereby developing and growing adventitious roots, and gradually forming a root system, which belongs to the cortex rooting type. way of raising seedlings.
本发明相对于现有技术具有如下的优点及效果:Compared with the prior art, the present invention has the following advantages and effects:
(1)本发明以柚木无根组培瓶苗为繁殖材料、选取育苗轻型基质和用育苗穴盘代替育苗袋进行快速育苗培植,实现了轻基质+育苗穴盘的快速工厂化育苗,装盘快,1人1天可配基质并装200盘(50孔/盘),而装营养土袋仅3000个;培育的营养土袋苗每株重0.50~0.75㎏,而轻基质苗每株仅重0.15~0.20㎏;本发明使育苗成本从营养土袋的0.932元/株降低到0.898元/株。因此,该方法节约了育苗和造林成本,提高育苗效率和造林效率,同时降低运输成本和造林工人的劳动强度。(1) The present invention uses teak rootless tissue culture bottle seedlings as propagation materials, selects a light substrate for seedling cultivation and replaces seedling cultivation bags with seedling cultivation trays for rapid seedling cultivation, and realizes rapid industrial seedling cultivation of light substrate + seedling cultivation trays, and puts them into trays Fast, one person can prepare substrate and pack 200 plates (50 holes/plate) per day, but only 3,000 nutritious soil bags are filled; the weight of cultivated nutrient soil bag seedlings is 0.50-0.75 kg per plant, while each plant of light matrix seedlings only The weight is 0.15-0.20 kg; the invention reduces the cost of raising seedlings from 0.932 yuan/plant in a nutrient soil bag to 0.898 yuan/plant. Therefore, the method saves seedling raising and afforestation costs, improves seedling raising efficiency and afforestation efficiency, and simultaneously reduces transportation costs and labor intensity of afforestation workers.
(2)本发明的柚木无根幼苗在移栽后7天即开始生根,与现有的用营养土袋的柚木育苗技术相比,本方法生根时间提前了3天,生根时间集中,生根成活率从87.6%提高到95.3%。(2) The rootless teak seedlings of the present invention begin to take root 7 days after transplanting. Compared with the existing teak seedling cultivation technology using nutrient soil bags, the rooting time of this method is advanced by 3 days, the rooting time is concentrated, and the rooting and survival The rate increased from 87.6% to 95.3%.
(3)本发明采用柚木无根组培瓶苗直接进行移栽,和组培的增殖系数一样,繁殖系数高,同时保持了柚木优良无性系的遗传特性;本发明操作方法简便、设备要求简单,技术容易掌握,易于推广,育苗时间较短,移栽3~4个月后即可出圃造林,可快速为市场提供大量的良种壮苗。(3) The present invention uses teak rootless tissue culture bottle seedlings to directly transplant, which is the same as the multiplication coefficient of tissue culture, and the multiplication coefficient is high, while maintaining the genetic characteristics of teak excellent clones; the present invention has simple operation methods and simple equipment requirements , the technology is easy to master, easy to popularize, and the time for seedling raising is short. After 3 to 4 months of transplanting, it can be out of the nursery for afforestation, and can quickly provide a large number of strong seedlings of good varieties for the market.
附图说明Description of drawings
图1是实施例1的柚木组培瓶苗的图。Fig. 1 is the figure of the teak tissue culture bottle seedling of embodiment 1.
图2是实施例1的修剪掉基部的愈伤组织后得到的柚木幼苗的图。Fig. 2 is the picture of the teak seedling obtained after pruning off the callus at the base of Example 1.
图3是实施例1用的育苗盘的图。Fig. 3 is a diagram of a seedling raising tray used in Example 1.
图4是实施例1中移栽1周后的柚木苗的图。Fig. 4 is the figure of the teak seedling after transplanting 1 week in embodiment 1.
图5是实施例1中移栽2个月后的柚木苗的图。Fig. 5 is the figure of the teak seedling after transplanting 2 months in embodiment 1.
图6是实施例1中移栽4个月后的柚木苗的图。Fig. 6 is the figure of the teak seedling after transplanting 4 months in embodiment 1.
图7是用于造林的1年生的柚木苗的图。Fig. 7 is a diagram of 1-year-old teak seedlings used for afforestation.
具体实施方式Detailed ways
下面结合实施例及附图对本发明作进一步详细的描述,但本发明的实施方式不限于此。The present invention will be further described in detail below in conjunction with the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.
实施例1Example 1
一种柚木无性系轻基质穴盘快速育苗方法(广州,5~6月进行):A method for rapid seedling cultivation of teak clones in light-substrate plugs (Guangzhou, carried out from May to June):
(1)繁殖材料的准备(1) Preparation of propagation materials
在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,将未经过生根培养、基部为愈伤组织的柚木(Tectona grandis L.f.)组培瓶苗(培养到3对叶、高7cm;取自中国林业科学研究院热带林业研究所,下同。)移到荫棚下练苗12天,然后从瓶中取出组培苗,剪去基部愈伤组织,得到幼苗,移栽前将幼苗浸泡于清水中;Teak (Tectona grandis L.f.) tissue culture bottle seedlings (cultivated to 3 Opposite leaves, 7cm high; taken from the Tropical Forestry Research Institute of the Chinese Academy of Forestry, the same below.) Moved to the shade shed to practice seedlings for 12 days, then took out the tissue culture seedlings from the bottle, cut off the base callus, and obtained the seedlings. Soak the seedlings in clean water before transplanting;
(2)育苗轻基质的准备(2) Preparation of light substrate for seedling cultivation
将泥炭土、蛭石、珍珠岩、椰糠和黄心土按体积比1︰1︰1︰2︰2混合均匀,得到育苗轻基质;Mix peat soil, vermiculite, perlite, coco peat and yellow heart soil evenly in a volume ratio of 1:1:1:2:2 to obtain a light substrate for seedling cultivation;
(3)育苗盘的选择(3) Selection of seedling trays
选取50孔塑料育苗盘(长宽高540mm×280mm×110mm),穴孔上大(46mm)下小,下部缩小为圆形(直径10mm)排水孔;Select a 50-hole plastic seedling tray (length, width, height 540mm×280mm×110mm), the holes are large on the top (46mm) and small on the bottom, and the bottom is reduced to a circular (diameter 10mm) drainage hole;
(4)育苗轻基质装盘与消毒(4) Packing and disinfection of light substrate for seedling cultivation
将育苗轻基质装满育苗盘后置于高20cm的铁架上,在移栽前1天用3wt‰的高猛酸钾水溶液浇透消毒,盖上薄膜备用;Fill the seedling-raising tray with the seedling-raising light substrate and place it on a 20cm-high iron frame, and sterilize it with 3wt‰ potassium permanganate aqueous solution 1 day before transplanting, and cover it with a film for subsequent use;
(5)移栽(5) Transplanting
移栽在阴天,在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,捞起步骤(1)中浸泡于清水中的幼苗,先用竹签在每穴育苗轻基质中央插1小孔,孔深1.5~2.5cm,将幼苗轻轻植入孔中,再用竹签在幼苗一侧压实,移栽完后喷清水定根,然后用1/800的百菌清水溶液喷雾消毒,最后盖上薄膜保湿,得到保湿的幼苗;Transplant on cloudy days, under the conditions of shading degree of 70-90%, humidity of 80-100%, and temperature of 25-28°C, pick up the seedlings soaked in clear water in step (1), and first use bamboo sticks on each Insert a small hole in the center of the light substrate for hole seedling cultivation, the hole depth is 1.5-2.5cm, gently implant the seedlings into the hole, and then use bamboo sticks to compact on one side of the seedlings. After transplanting, spray water to fix the roots, and then use 1/ 800% chlorothalonil aqueous solution was sprayed for disinfection, and finally covered with a film for moisturizing to obtain moisturizing seedlings;
(6)后期管理(6) Later management
于移栽后第3天对步骤(5)中保湿的幼苗进行第二次消毒,之后每周消毒1次;消毒为采用1/800甲基托布津水溶液、1/800百菌清水溶液或1/800多菌灵水溶液交替消毒;晴天每天喷水2~3次,分别为上午9点,下午6点,另1次可根据情况定,以保持幼苗叶片湿润不萎蔫为准,太阳光照强,温度高于30℃时,需要在薄膜上加盖遮阳网,10点~17点每隔1小时膜外喷水1次,保持遮荫度70~90%、湿度80~100%、温度25~28℃的条件;阴天可于下午5点喷1次或根据苗情不喷,雨天不喷;移栽20天后浇施1.5wt‰的尿素,移栽1.5月后,消毒浓度可增加到4/800,追肥浓度可增加到4wt‰,此时晴天或阴天可揭开薄膜练苗,雨天也可以打开薄膜两端通气;On the 3rd day after transplanting, the seedlings kept moist in step (5) were sterilized for the second time, and then sterilized once a week; disinfection was performed with 1/800 thiophanate-methyl aqueous solution, 1/800 chlorothalonil aqueous solution or 1 /800 carbendazim aqueous solution alternately disinfected; spray water 2 to 3 times a day on sunny days, respectively at 9:00 am and 6:00 pm, and the other time can be determined according to the situation, so as to keep the leaves of the seedlings moist and not wilting. When the temperature is higher than 30°C, it is necessary to cover the film with a sunshade net, and spray water on the outside of the film every hour from 10:00 to 17:00 to keep the shading degree of 70-90%, humidity of 80-100%, and temperature of 25- Conditions at 28°C; spray once at 5 pm on cloudy days or do not spray according to seedling conditions, and do not spray on rainy days; 1.5wt‰ of urea is applied after 20 days of transplanting, and the disinfection concentration can be increased to 4 after 1.5 months of transplanting /800, the topdressing concentration can be increased to 4wt‰, at this time, the film can be uncovered for seedling training in sunny or cloudy days, and both ends of the film can be opened for ventilation in rainy days;
移栽后生长情况:Growth after transplanting:
移栽1周即开始生根,此时幼苗已进入稳定期,2周后大量生根或苗木发根齐全,此时认为移栽成活,之后根系伸长,侧根增多,逐渐形成成熟完整的根系,成活率达到95.3%;移栽4个月苗高达20cm,出圃造林。Rooting begins within 1 week of transplanting. At this time, the seedlings have entered a stable period. After 2 weeks, a large number of roots have taken root or the seedlings have fully developed roots. At this time, the transplanting is considered to be alive. The rate reached 95.3%; the seedlings were up to 20cm in 4 months after transplanting, and they were planted out of the nursery.
实施例2Example 2
一种柚木无性系轻基质穴盘快速育苗方法(广州,4月下旬进行):A method for rapid seedling cultivation of teak clones in light-substrate plugs (Guangzhou, carried out in late April):
(1)繁殖材料的准备(1) Preparation of propagation materials
在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,将未经过生根培养、基部为愈伤组织的柚木(Tectona grandis L.f.)组培瓶苗(培养到2对叶、高5cm)移到荫棚下练苗15天,然后从瓶中取出组培苗,剪去基部愈伤组织,得到幼苗,移栽前将幼苗浸泡于清水中;Under the conditions of shading degree of 70-90%, humidity of 80-100%, and temperature of 25-28°C, teak (Tectona grandis L.f.) tissue culture bottle seedlings (cultivated to 2 Opposite leaves, height 5cm) were moved to the shade for 15 days, and then the tissue cultured seedlings were taken out from the bottle, and the base callus was cut off to obtain seedlings, which were soaked in clear water before transplanting;
(2)育苗轻基质的准备(2) Preparation of light substrate for seedling cultivation
将泥炭土、蛭石、珍珠岩、椰糠和黄心土按体积比1︰1︰1︰2︰2混合均匀,得到育苗轻基质;Mix peat soil, vermiculite, perlite, coco peat and yellow heart soil evenly in a volume ratio of 1:1:1:2:2 to obtain a light substrate for seedling cultivation;
(3)育苗盘的选择(3) Selection of seedling trays
选取50孔塑料育苗盘(长宽高540mm×280mm×110mm),穴孔上大(46mm)下小,下部缩小为圆形(直径10mm)排水孔;Select a 50-hole plastic seedling tray (length, width, height 540mm×280mm×110mm), the holes are large on the top (46mm) and small on the bottom, and the bottom is reduced to a circular (diameter 10mm) drainage hole;
(4)育苗轻基质装盘与消毒(4) Packing and disinfection of light substrate for seedling cultivation
将育苗轻基质装满育苗盘后置于高20cm的铁架上,在移栽前0.5天用3wt‰的高猛酸钾水溶液浇透消毒,盖上薄膜备用;Fill the seedling raising tray with the light medium for seedling raising and place it on a 20cm high iron frame, and sterilize it with 3wt‰ potassium permanganate aqueous solution 0.5 days before transplanting, and cover it with a film for subsequent use;
(5)移栽(5) Transplanting
移栽在阴天,在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,捞起步骤(1)中浸泡于清水中的幼苗,先用竹签在每穴育苗轻基质中央插1小孔,孔深1.5~2.5cm,将幼苗轻轻植入孔中,再用竹签在幼苗一侧压实,移栽完后喷清水定根,然后用1/800的百菌清水溶液喷雾消毒,最后盖上薄膜保湿,得到保湿的幼苗;Transplant on cloudy days, under the conditions of shading degree of 70-90%, humidity of 80-100%, and temperature of 25-28°C, pick up the seedlings soaked in clear water in step (1), and first use bamboo sticks on each Insert a small hole in the center of the light substrate for hole seedling cultivation, the hole depth is 1.5-2.5cm, gently implant the seedlings into the hole, and then use bamboo sticks to compact on one side of the seedlings. After transplanting, spray water to fix the roots, and then use 1/ 800% chlorothalonil aqueous solution was sprayed for disinfection, and finally covered with a film for moisturizing to obtain moisturizing seedlings;
(6)后期管理(6) Later management
于移栽后第4天对步骤(5)中保湿的幼苗进行第二次消毒,之后每周消毒1次;消毒为采用1/800甲基托布津水溶液、1/800百菌清水溶液或1/800多菌灵水溶液交替消毒;晴天每天喷水2~3次,分别为上午9点,下午6点,另1次可根据情况定,以保持幼苗叶片湿润不萎蔫为准,太阳光照强,温度高于30℃时,需要在薄膜上加盖遮阳网,10~17点每隔1小时膜外喷水1次,保持遮荫度70~90%、湿度80~100%、温度25~28℃的条件;阴天可于下午5点喷1次或根据苗情不喷,雨天不喷;移栽25天后浇施1wt‰的尿素,移栽1.5月后,消毒浓度可增加到3/800,追肥浓度可增加到3wt‰,此时晴天或阴天可揭开薄膜练苗,雨天也可以打开薄膜两端通气;On the 4th day after transplanting, the seedlings moistened in step (5) were sterilized for the second time, and then sterilized once a week; disinfection was performed with 1/800 thiophanate-methyl aqueous solution, 1/800 chlorothalonil aqueous solution or 1 /800 carbendazim aqueous solution alternately disinfected; spray water 2 to 3 times a day on sunny days, respectively at 9:00 am and 6:00 pm, and the other time can be determined according to the situation, so as to keep the leaves of the seedlings moist and not wilting. When the temperature is higher than 30°C, it is necessary to cover the film with a sunshade net, and spray water once every hour from 10 to 17 o'clock to keep the shading degree of 70-90%, the humidity of 80-100%, and the temperature of 25-28 ℃ conditions; cloudy days can be sprayed once at 5 pm or not sprayed according to the condition of the seedlings, and not sprayed on rainy days; 25 days after transplanting, water 1wt‰ of urea, and after 1.5 months of transplanting, the disinfection concentration can be increased to 3/800 , the topdressing concentration can be increased to 3wt‰, at this time, the film can be uncovered to practice seedlings on sunny or cloudy days, and both ends of the film can be opened to ventilate in rainy days;
移栽后生长情况:Growth after transplanting:
移栽1周即开始生根,此时幼苗已进入稳定期,2周后大量生根或苗木发根齐全,此时认为移栽成活,之后根系伸长,侧根增多,逐渐形成成熟完整的根系,成活率达到95.3%;移栽3个月苗高达15cm,出圃造林。Rooting begins within 1 week of transplanting. At this time, the seedlings have entered a stable period. After 2 weeks, a large number of roots have taken root or the seedlings have fully developed roots. At this time, the transplanting is considered to be alive. The rate reached 95.3%; the seedlings were up to 15cm in 3 months after transplanting, and they were planted out of the nursery.
实施例3Example 3
一种柚木无性系轻基质穴盘快速育苗方法(广州,7月中旬进行):A method for rapid seedling cultivation of teak clones in light-substrate plugs (Guangzhou, carried out in mid-July):
(1)繁殖材料的准备(1) Preparation of propagation materials
在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,将未经过生根培养、基部为愈伤组织的柚木(Tectona grandis L.f.)组培瓶苗(培养到4对叶、高10cm)移到荫棚下练苗10天,然后从瓶中取出组培苗,剪去基部愈伤组织,得到幼苗,移栽前将幼苗浸泡于清水中;Under the conditions of shading degree of 70-90%, humidity of 80-100%, and temperature of 25-28°C, teak (Tectona grandis L.f.) tissue culture bottle seedlings (cultivated to 4 Opposite leaves, height 10cm) moved to the shade for 10 days, then took out the tissue-cultured seedlings from the bottle, cut off the base callus to obtain seedlings, soaked the seedlings in clear water before transplanting;
(2)育苗轻基质的准备(2) Preparation of light substrate for seedling cultivation
将泥炭土、蛭石、珍珠岩、椰糠和黄心土按体积比1︰1︰1︰2︰2混合均匀,得到育苗轻基质;Mix peat soil, vermiculite, perlite, coco peat and yellow heart soil evenly in a volume ratio of 1:1:1:2:2 to obtain a light substrate for seedling cultivation;
(3)育苗盘的选择(3) Selection of seedling trays
选取50孔塑料育苗盘(长宽高540mm×280mm×110mm),穴孔上大(46mm)下小,下部缩小为圆形(直径10mm)排水孔;Select a 50-hole plastic seedling tray (length, width, height 540mm×280mm×110mm), the holes are large on the top (46mm) and small on the bottom, and the bottom is reduced to a circular (diameter 10mm) drainage hole;
(4)育苗轻基质装盘与消毒(4) Packing and disinfection of light substrate for seedling cultivation
将育苗轻基质装满育苗盘后置于高15cm的铁架上,在移栽前1天用3wt‰的高猛酸钾水溶液浇透消毒,盖上薄膜备用;Fill the seedling raising tray with the light medium for seedling raising and place it on a 15cm high iron frame, and sterilize it with 3wt‰ potassium permanganate aqueous solution 1 day before transplanting, and cover it with a film for subsequent use;
(5)移栽(5) Transplanting
移栽在晴天,在遮荫度70~90%、湿度80~100%、温度25~28℃的条件下,捞起步骤(1)中浸泡于清水中的幼苗,先用竹签在每穴育苗轻基质中央插1小孔,孔深1.5~2.5cm,将幼苗轻轻植入孔中,再用竹签在幼苗一侧压实,移栽完后喷清水定根,然后用1/800的百菌清水溶液喷雾消毒,最后盖上薄膜保湿,得到保湿的幼苗;Transplant on a sunny day, under the conditions of shading degree of 70-90%, humidity of 80-100%, and temperature of 25-28°C, pick up the seedlings soaked in clear water in step (1), and first use bamboo sticks in each hole. Insert a small hole in the center of the light medium for seedling cultivation, the hole depth is 1.5-2.5cm, gently implant the seedlings into the hole, and then use a bamboo stick to compact the side of the seedlings, spray water to fix the roots after transplanting, and then use 1/800 The chlorothalonil aqueous solution spray disinfection, and finally cover the film moisturizing, to obtain moisturizing seedlings;
(6)后期管理(6) Later management
于移栽后第4天对步骤(5)中保湿的幼苗进行第二次消毒,之后每周消毒1次;消毒为采用1/800甲基托布津水溶液、1/800百菌清水溶液或1/800多菌灵水溶液交替消毒;晴天每天喷水2~3次,分别为上午9点,下午6点,另1次可根据情况定,以保持幼苗叶片湿润不萎蔫为准,太阳光照强,温度高于30℃时,需要在薄膜上加盖遮阳网,10~17点每隔1小时膜外喷水1次,保持遮荫度70~90%、湿度80~100%、温度25~28℃的条件;阴天可于下午5点喷1次或根据苗情不喷,雨天不喷;移栽25天后浇施1wt‰的复合肥,移栽1.5月后,消毒浓度可增加到5/800,追肥浓度可增加到5wt‰,此时晴天或阴天可揭开薄膜练苗,雨天也可以打开薄膜两端通气;On the 4th day after transplanting, the seedlings moistened in step (5) were sterilized for the second time, and then sterilized once a week; disinfection was performed with 1/800 thiophanate-methyl aqueous solution, 1/800 chlorothalonil aqueous solution or 1 /800 carbendazim aqueous solution alternately disinfected; spray water 2 to 3 times a day on sunny days, respectively at 9:00 am and 6:00 pm, and the other time can be determined according to the situation, so as to keep the leaves of the seedlings moist and not wilting. When the temperature is higher than 30°C, it is necessary to cover the film with a sunshade net, and spray water once every hour from 10 to 17 o'clock to keep the shading degree of 70-90%, the humidity of 80-100%, and the temperature of 25-28 ℃ conditions; cloudy days can be sprayed once at 5 p.m. or not sprayed according to seedling conditions, and not sprayed on rainy days; 1wt‰ of compound fertilizer is applied after 25 days of transplanting, and the disinfection concentration can be increased to 5/‰ after transplanting for 1.5 months. 800, the topdressing concentration can be increased to 5wt‰, at this time, the film can be uncovered to practice seedlings on sunny or cloudy days, and both ends of the film can be opened to ventilate in rainy days;
移栽后生长情况:Growth after transplanting:
移栽1周即开始生根,此时幼苗已进入稳定期,2周后大量生根或苗木发根齐全,此时认为移栽成活,之后根系伸长,侧根增多,逐渐形成成熟完整的根系,成活率达到95.3%;移栽4个月苗高达15cm,出圃造林。Rooting begins within 1 week of transplanting. At this time, the seedlings have entered a stable period. After 2 weeks, a large number of roots have taken root or the seedlings have fully developed roots. At this time, the transplanting is considered to be alive. The rate reached 95.3%; the seedlings were up to 15cm in 4 months after transplanting, and they were planted out of the nursery.
上述实施例为本发明较佳的实施方式,但本发明的实施方式并不受上述实施例的限制,其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化,均应为等效的置换方式,都包含在本发明的保护范围之内。The above-mentioned embodiment is a preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes, modifications, substitutions, combinations, Simplifications should be equivalent replacement methods, and all are included in the protection scope of the present invention.
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