CN102960374A - New application of cortex moutan radicis ethanol extract in weeding - Google Patents
New application of cortex moutan radicis ethanol extract in weeding Download PDFInfo
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- CN102960374A CN102960374A CN2012105513705A CN201210551370A CN102960374A CN 102960374 A CN102960374 A CN 102960374A CN 2012105513705 A CN2012105513705 A CN 2012105513705A CN 201210551370 A CN201210551370 A CN 201210551370A CN 102960374 A CN102960374 A CN 102960374A
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- weeding
- ethanol extract
- cortex moutan
- moutan bark
- new application
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Abstract
The invention relates to the field of pesticides, and particularly discloses a new application of a cortex moutan radicis ethanol extract in weeding. Pharmacological research indicates that the cortex moutan radicis ethanol extract plays a complete inhibition role on Brassica rapa L.Chinensis Group under certain concentration and can be used as a plant biopesticide and a herbicide.
Description
Technical field
The present invention relates to pesticide field, be specially the new purposes of a kind of moutan bark alcohol extract aspect weeding.
Background technology
The weeds that the whole world extensively distributes have 30000 kinds, and annual about 1800 kinds are caused in various degree harm to crop, and the annual crop production reduction that causes because of weeds harm is up to 9.7%.
Although adopt chemical herbicide effectively to control many weeds, a large amount of uses of chemical agent have also caused a lot of bad problems, such as the degeneration of the appearance of herbicide-resistant weeds plant, soil pollution, water quality and to the harm of people, animal etc.
Along with the raising of people's environmental consciousness and the needs of agricultural sustainable development, the research of efficient, environmental protection, harmless biological weed killer more and more is subject to people's attention.As the plant resource weed killer herbicide of biological weed killer part, also be study hotspot in recent years, wherein, qinghaosu and Cineole are subject to the people's attention because of the good action that it has good weeding.
Moutan bark is the dry root skin of ranunculaceae peony.Excavate root autumn, remove radicula, strip the root skin, dry.Give birth to or fry and use.Another name: moutan root bark, the root bark of tree peony, red root.Nature and flavor: arduous; Cool; Be slightly cold.Gui Jing: the heart channel of Hang-Shaoyin; Liver Channel; Kidney channel; Lung channel.Function: heat-clearing; Promoting blood circulation to remove blood stasis.Cure mainly: the warm heat disease heat invading xuefen; Send out spot; Tell nosebleed; The germ later stage brimful of tears, hot Fu Yin divided heating; Deficiency of Yin osteopyrexia and fever; Amenorrhea due to stagnation of blood; Dysmenorrhoea; Carbuncle sore tumefacting virus; Fall and pounce on the pain of injury; Rheumatic fever numbness.Originate in the ground such as Anhui, Sichuan, Gansu, Shaanxi, Hubei, Hunan, Guizhou, Shandong.Compendium of Material Medica: " nourishing Yin and falling fire, separate the spot poison, throat, diuresis stasis.The descendant specially controls mutually fire with yellow tiller, does not know that the merit of the root bark of tree peony more wins also.Red colored person is sharp, and the person of spending in vain mends, and the people also seldom realizes, and should distinguish it." modern study, contained paeonol and glucosides constituents in addition thereof all have antiinflammatory action; The methanolic extract of moutan bark has the inhibition Platelet; Paeonol has the effects such as the central inhibitory actions such as calmness, cooling, analgesic, analgesia, spasmolysis and antiatherosclerosis, diuresis, antiulcer.
Summary of the invention
The invention discloses a kind of new purposes of moutan bark alcohol extract, namely it is used for the new purposes of weeding aspect.Evidence, the moutan bark alcohol extract has selectivity aspect weeding, especially Brassica rapa L.Chinensis Group (rape) is had single-minded inhibitory action.
After referring to moutan bark and (60-100%) ethanol 1: 8～10 (W/V) refluxed 2 hours, filters the moutan bark alcohol extract of chatting, filter residue filters after 2 hours with 8 times of amounts (60-100%) alcohol reflux again, reduced pressure concentration after filtrate merges, 60 degree are lower dry, the brown medicinal extract of gained.
Embodiment
Below in conjunction with specific embodiment moutan bark alcohol extract of the present invention is elaborated in the application aspect the agricultural chemicals.
Test sample: the moutan bark medicinal material is purchased from Nanjing medicine company limited company.
Experimental instrument: high speed Universalpulverizer; Vertical automatic pressure steam sterilizer; Shaking table; The ultrasonic oscillation instrument; Rotary Evaporators; Thermostat water bath; Electronic balance; The intelligence climatic cabinate; Vavuum pump.
Mother liquor configuration: get moutan bark alcohol extract (brown medicinal extract) with the DMSO dissolving, be prepared into the mother liquor that concentration is 10mg/mL.
The configuration of test solution: during use mother liquor is diluted to debita spissitudo with 0.025%Tween 20.
Careless for examination: rape (Brassica rapa L.Chinensis Group), Bermuda grass (Cynodon dactylon L.), duckweed (Lemna minor) are provided by the academy of agricultural sciences, Hubei Province.
Test method: screening technique is agar medium surface-coated method when adopting a variety of Chinese cabbage and Bermuda grass to be target.Adopt 96 porocyte culture plates, every hole adds entry agar 300 μ L and about 10 seeds, then adds dilute liquid medicine 20 μ L, and the primary dcreening operation liquor strength is 1.0mg/mL, 0.3mg/mL, 0.1mg/mL.After one week, check seed germination and plant strain growth situation.Sieving again concentration adjusts according to the primary dcreening operation result.
Adopt water culture during take duckweed as target.Adopt 24 porocyte culture plates, every hole adds duckweed and cultivates nutrient solution 2mL, accesses 3 duckweed blades, adds dilute liquid medicine 20 μ L again.Liquor strength is 1.0mg/mL during primary dcreening operation, checks the growing state of duckweed after the week.Sieving again concentration adjusts according to the primary dcreening operation result.
When sieving again, be 60ug/cm to the concentration of rape and Bermuda grass
2, be 10ppm to the concentration of duckweed.
Test sample: the moutan bark medicinal material is purchased from Nanjing medicine company limited company.
Experimental result shows that the moutan bark alcohol extract is at 60ug/cm
2Down rape is suppressed fully, duckweed and Bermuda grass are not had inhibitory action, have certain selectivity.
Claims (3)
1. the application of moutan bark alcohol extract aspect agricultural chemicals.
2. application according to claim 1 is characterized in that: described moutan bark alcohol extract is used for the new purposes of weeding aspect.
3. application according to claim 2 is characterized in that: described moutan bark alcohol extract adopts first DMSO dissolving preparation mother liquor when using, and gets described mother liquor before using and is 60ug/cm with the application concentration that 0.025% polysorbas20 is diluted to the moutan bark alcohol extract
2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105513705A CN102960374A (en) | 2012-12-18 | 2012-12-18 | New application of cortex moutan radicis ethanol extract in weeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105513705A CN102960374A (en) | 2012-12-18 | 2012-12-18 | New application of cortex moutan radicis ethanol extract in weeding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102960374A true CN102960374A (en) | 2013-03-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105513705A Pending CN102960374A (en) | 2012-12-18 | 2012-12-18 | New application of cortex moutan radicis ethanol extract in weeding |
Country Status (1)
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| CN (1) | CN102960374A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008141252A1 (en) * | 2007-05-10 | 2008-11-20 | Jh Biotech, Inc. | Organic herbicide |
| WO2009049153A2 (en) * | 2007-10-11 | 2009-04-16 | Marrone Organic Innovations, Inc. | A natural herbicide containing lemongrass essential oil |
| CN102246831A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Peony bark nematicidal extract and preparation method thereof |
-
2012
- 2012-12-18 CN CN2012105513705A patent/CN102960374A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008141252A1 (en) * | 2007-05-10 | 2008-11-20 | Jh Biotech, Inc. | Organic herbicide |
| WO2009049153A2 (en) * | 2007-10-11 | 2009-04-16 | Marrone Organic Innovations, Inc. | A natural herbicide containing lemongrass essential oil |
| CN102246831A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Peony bark nematicidal extract and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 孙文琰 等: "茜草等23种植物对棉铃虫生物活性的研究", 《河北农业大学学报》, vol. 31, no. 5, 31 October 2008 (2008-10-31), pages 66 - 70 * |
| 王留兴: "牡丹皮乙醇提取工艺研究", 《长春中医药大学学报》, vol. 24, no. 1, 29 February 2008 (2008-02-29), pages 35 - 36 * |
| 王立 等: "牡丹皮药材的最适提取工艺研究", 《中国中药杂志》, vol. 30, no. 8, 30 April 2005 (2005-04-30), pages 569 - 571 * |
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| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20130313 |