CN102899330A - Transforming growth factor-beta (TGF-beta) type I receptor gene of chlamys farreri and single nucleotide polymorphism (SNP) locus of TGF-beta type I receptor gene - Google Patents
Transforming growth factor-beta (TGF-beta) type I receptor gene of chlamys farreri and single nucleotide polymorphism (SNP) locus of TGF-beta type I receptor gene Download PDFInfo
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- CN102899330A CN102899330A CN2012104398610A CN201210439861A CN102899330A CN 102899330 A CN102899330 A CN 102899330A CN 2012104398610 A CN2012104398610 A CN 2012104398610A CN 201210439861 A CN201210439861 A CN 201210439861A CN 102899330 A CN102899330 A CN 102899330A
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Abstract
The invention relates to the cloning of a transforming growth factor-beta (TGF-beta) superfamily type I receptor gene Tgfbrl1 of chlamys farreri in a molecular genetic marker technology, a screening and typing technology of a single nucleotide polymorphism (SNP) locus which is relevant with the weight of adductor muscles in the gene and a method for the application of the gene to the breeding of the high-yield chlamys farreri. A total-length complementary deoxyribonucleic acid (cDNA) sequence of the Tgfbr1 gene of the chlamys farreri is obtained by utilizing a homology-based cloning strategy; an SNP lotus is discovered by blastn comparison, and primers and a probe are designed for the locus; and SNP typing is performed in a natural population of the chlamys farreri by using a high-resolution melting curve technology, and the weight of the adductor muscle of an individual is measured. Statistic analysis displays that the loci c.1815C>T are obviously relevant with the weight of the adductor muscles of the chlamys farreri, and the weight of the adductor muscles of individuals with TT genetypes is obviously higher than that of the adductor muscles of individuals with CC and CT genetypes. In the selective breeding process of the high-yield chlamys farreri, the breeding candidate colonies of the chlamys farreri can be subjected to c.1815C>T typing, and the individuals of which the loci c.1815C>T are TT genetypes are used as a breeding parent preferably by combining typing information of other loci which are relevant with growth properties.
Description
Technical field
The invention belongs to molecular genetic marker assistant breeding technical field, relate to the clone of chlamys farreri TGF-β I receptor gene Tgfbr1, in the gene with the examination typing method in closed shell flesh weight related SNP site, and the method for in the seed selection of high yield scallop, using.
Background technology
Transforming growth factor-beta (transforming growth factor β, TGF-β) signal path plays a significant role in the biological procedureses such as cell proliferation, differentiation and growth.The TGF-beta ligands passes through in conjunction with TGF-β I type and II receptor, and the intracellular signal molecule in downstream, passes the signal along in the nuclear expression of goal of regulation and control gene.Wherein the activation of I receptor is considered to the important step that the TGF-signal β transmits.Studies show that in Mammals, the growth of Tgfbr1 gene and cell and rise in value relevantly, the single nucleotide polymorphism in this gene (SNP) site is relevant with many growth traitss.
In the marine products eight delicacies, scallop is celebrated with its large and delicious closed shell flesh (cedductor).Cultivating high yield scallop new variety is needs of scallop culture industry fast development.Obtain gene and the gene locus relevant with scallop growth traits, particularly closed shell flesh weight and will help its molecular breeding.At present, gene or the gene locus relevant with growth traits that obtain in scallop are less, and there is no the report of TGF-signal β path acceptor gene.
Summary of the invention:
The objective of the invention is to clone chlamys farreri TGF-β I receptor gene, with the SNP site of closed shell flesh re-correlation, provide the technological method of SNP mark and application thereof for the seed selection of high yield chlamys farreri in the examination gene.
One aspect of the invention provides a kind of chlamys farreri TGF-β I receptor gene, and its nucleotides sequence is classified SEQ ID NO:1 as.
Above-mentioned TGF-β I receptor gene, the aminoacid sequence of its coding is SEQ ID NO:2.
Another aspect of the present invention provides the SNP site relevant with closed shell flesh weight of chlamys farreri TGF-β I receptor gene, is that sequence is that 1815 base of SEQ ID NO:1 gene is C or T.
The SNP of above-mentioned chlamys farreri TGF-β I receptor gene is used for the breeding seed selection of chlamys farreri.
Probe and primer sequence information for detection of above-mentioned SNP are as follows:
Probe: 5 '-TTGTAAGGAAGTCGGATACCGAACT-3 ' SEQ ID NO:2;
Upstream primer: 5 '-GAGGTGTCAGACTGATTTTCAGGAG-3 ' SEQ ID NO:3;
Downstream primer: 5 '-ATGTCACAAAGGAAATTCATAAAGC-3 ' SEQ ID NO:4.
The SNP site of the present invention's screening is the chlamys farreri of TT type, closed shell flesh weight is significantly higher than CC type and CT type chlamys farreri, in the Breeding Process of high yield chlamys farreri, and can be for this site, the preferential individuality of selecting the TT type is as breeding parent, to improve the efficient of seed selection.
Description of drawings
Fig. 1: the chlamys farreri TGF-β I receptor full length gene cDNA sequence of the present invention's screening and the aminoacid sequence of coding thereof;
Wherein the signal peptide sequence cleavage site marks with arrow, 10 conservative cysteine residues and cross-film district mark with shade, the GS district marks with underscore, the ATP land marks with shade and underscore, L45 loop marks with black surround, introne position marks with black triangle, and terminator codon represents with asterisk, and tailing signal marks with shade and black surround.SNP site c. 1815 C〉T represents with bold Italic.
Embodiment
The present invention adopts homologous clone and terminal rapid amplifying (RACE) technology of cDNA, and the clone has obtained TGF-beta superfamily I receptor gene cDNA full length sequence from chlamys farreri.This sequence total length 1908 bp, ORF length is 1575 bp, 524 amino acid of encoding, predicted protein molecular weight 59.27 KD, iso-electric point is 7.77; 5 ' UTR length is 15 bp, and 5 ' UTR length is 318 bp.By one section 30 signal peptide sequence that amino-acid residue forms, at 142-164 amino acids place one section cross-film district is arranged at its proteins encoded N-terminal.Extracellular region has 10 conservative cysteine residues, and wherein 3 form typical CCX near the cross-film district
5C saves structure.It mainly is the serine/threonine kinase district in the born of the same parents, conservative property is higher, the typical structure GS district that comprises the I receptor, the ATP at 229-250 amino acids place are in conjunction with the position district, and the L45 loop (ADNKDGTW) of acceptor-Smad binding specificity is decided in the execution of 283-291 amino acids.
In the chlamys farreri Tgfbr1 gene with the Screening analysis in closed shell flesh re-correlation SNP site and the method for auxiliary high yield chlamys farreri seed selection thereof:
By the Tgfbr1 gene cDNA sequence that analysis obtains, examination to 1 a SNP site, called after is c.1815C〉T.Use high resolving power melting curve technology (High resolution melting, HRM) detection site polymorphism in natural population of chlamys farreri, the result shows, in survey colony, this SNP site is two condition (Fig. 1).One-way ANOVA and post-hoc check demonstration, c.1815C〉the T site is relevant with chlamys farreri closed shell flesh representation work, and the closed shell flesh representation work of TT type individuality is higher than CC and CT type individual (P＜0.05) (table 1).Utilize the site c.1815C〉typing method of T, can carry out gene type to chlamys farreri breeding candidate colony, in conjunction with the somatotype information of other and growth traits related locus, preferentially selecting the site c.1815C〉T is the parent that the individuality of TT type is used for the seed selection of high yield scallop.
The present invention will be further described below by embodiment.
The clone obtains chlamys farreri TGF-beta superfamily I receptor gene Tgfbr1, has the sequence shown in the SEQ ID NO. 1.
Chlamys farreri Tgfbr1 gene cDNA sequence clone among the present invention comprises the following steps:
A) extraction of the total RNA of chlamys farreri closed shell flesh;
B) cDNA the first chain is synthetic;
C) acquisition of goal gene cDNA fragment;
D) chlamys farreri 5 ' RACE and 3 ' RACE library construction;
E) acquisition of goal gene full length cDNA sequence;
F) bioinformatic analysis of goal gene.
Concrete operations are as follows:
A) extraction of the total RNA of chlamys farreri: from chlamys farreri closed shell flesh, extract total RNA according to the Trizol method.
B) cDNA the first chain is synthetic: take the total RNA of 2 μ g as template, add respectively 1 μ l, 20 μ M Oligo d (T)
18, RNase ﹠amp; DNase-free H
2O complements to 13 μ l, and mixing is centrifugal.70 ℃ of insulation 10 min place on ice, immediately to open the RNA secondary structure.Add successively: 5 μ l, 5 * M-MLV Buffer, 5 μ l dNTP (2.5mM), 1 μ l RNasin (40U/ μ l), 1 μ l M-MLV (200U/ μ l); After mixing is centrifugal, 42 ℃, 90 min.94 ℃, 5 min are with the deactivation ThermoScript II.-20 ℃ of preservations after the packing.
C) homologous clone obtains the cDNA fragment of goal gene: according to TGF-β I receptor gene cDNA and the aminoacid sequence of the species such as the Pacific oyster of having reported, sea urchin, zebra fish, rainbow trout, Africa xenopus, jungle fowl, mouse and the mankind, select the high zone design degenerated primer of conservative property:
d)TGFBR1-f1:?5′-GAYAAYAARGAYAAYGGIACITGG-3′；
e)TGFBR1-r1:?5′-?GGIGCCATRTAICKYTTIGTIC-3′。
Then take cDNA the first chain as masterplate, carry out pcr amplification.The PCR product detects with 1.2% agarose gel electrophoresis, reclaim test kit (worker is given birth in Shanghai) with glue and carry out the PCR product purification, be connected with pMD18-T carrier (the precious biotechnology in Dalian company limited) again, (Huang Peitang translates with reference to " the molecular cloning third edition ", Science Press, 2002) method transform bacillus coli DH 5 alpha.After bacterium colony PCR detected, the picking positive colony checked order, and known array in sequencing result and the GenBank albumen database is made blastx homology analysis (http://blast.ncbi.nlm.nih.gov/Blast/).
F) chlamys farreri 5 ' RACE and 3 ' RACE library construction: utilize the SMART RACE cDNA Amplification Kit of Clontech company to make up 5 ' RACE and 3 ' RACE library.
G) acquisition of goal gene full length cDNA sequence: according to the Tgfbr1 gene cDNA fragment sequence that (c) obtains, use respectively purpose of design gene RACE primers of PrimerPremier 5.0,
3 ' ' RACE primer: 5 '-TCAACCGTACTGTGGTCAGCATCTCCG-3 '
5 ' RACE primer: 5 '-CCAATGATCTCCATGTGGAGGTGGGCG-3 '.
Utilize respectively 3 ' RACE primer and 5 ' RACE primer and joint primer NUP(5' – AAGCAGTGGTATCAACGCAGAGT – 3') carry out the amplification of 3 ' terminal and 5 ' end.The PCR product detects with 1.2% agarose gel electrophoresis, reclaim test kit (worker is given birth in Shanghai) with glue and carry out the PCR product purification, be connected with pMD18-T carrier (the precious biotechnology in Dalian company limited) again, (Huang Peitang translates with reference to " the molecular cloning third edition ", Science Press, 2002) method transform bacillus coli DH 5 alpha.After bacterium colony PCR detected, to 5 ' RACE and 3 ' RACE, 3 positive colonies of each picking checked order, and obtained the cDNA full length sequence after the sequence two ends splicing with 5 ' RACE and 3 ' RACE order-checking acquisition, saw SEQ ID NO. 1.
3 ' and 5 ' RLM-RACE, utilize 50 μ L reaction systems:
Used PCR response procedures increases: 94 ℃ of denaturation 5min; 94 ℃ of sex change 30sec, 72 ℃ of 3min, 5 circulations; 94 ℃ of sex change 30sec, 70 ℃ of annealing 30sec, 72 ℃ are extended 3min, 5 circulations; 94 ℃ of sex change 30sec, 65 ℃ of annealing 30sec, 72 ℃ are extended 3min, 28 circulations; 72 ℃ are extended 10min.
H) bioinformatic analysis of goal gene:
In ncbi database, this sequence is carried out BLASTx (http://blast.ncbi.nlm.nih.gov/) comparison.Use the ProtParam instrument SQR physics and chemistry parameter is predicted, comprise (www.expasy.ch/tools/protparam.html) such as the relative molecular weight of protein, theoretical pI values.By internet line server http://www.cbs.dtu.dk/services/SignalP/, TGF-beta superfamily I receptor protein sequence has been carried out the prediction of signal peptide.Use the functional domain of SMART (http://smart.embl-heidelberg.de/) this albumen of software prediction.Use Phyre 2.0 (http://www.imperial.ac.uk/phyre2/) and Swiss-Pdb Viewer 4.04 (http://spdbv.vital-it.ch/) to analyze the serine/threonine kinase district.Use ClustalW2 and GeneDoc (http://www.nrbsc.org/gfx/genedoc/index.html) Multiple Sequence Alignment instrument and other species TGF-beta superfamily I receptor protein sequence to compare, analyze this protein function zone.Analytical results as shown in Figure 1.
Embodiment 2
The Screening analysis in closed shell flesh weight related SNP site and the method for using in the seed selection of high yield chlamys farreri thereof comprise the following steps: in the chlamys farreri Tgfbr1 gene among the present invention
A) chlamys farreri Tgfbr1 gene SNP site examination;
B) scallop (Chlamys farreri) group DNA extraction;
C) site is c.1815C〉T somatotype primer and probe design;
D) SNP is c.1815C〉the T somatotype;
E) c.1815C〉the heavy correlation analysis of T genotype and closed shell flesh;
F) SNP is c.1815C〉method of auxiliary high yield chlamys farreri seed selection.
Concrete operations are as follows:
A) chlamys farreri Tgfbr1 gene SNP site examination: analyze 5 ' RACE clones' different from 3 ' RACE sequencing result, search candidate SNP locus.
B) extraction of chlamys farreri DNA: get approximately 0.1 g of closed shell flesh, add 500 μ l STE lysis buffers, shred, add successively 50 μ l, 10% SDS, 5 μ l Proteinase Ks (20 mg/ml), 56 ℃ of cracking are 3 h approximately, clarify to lysate.Adding is with the saturated phenol of volume (250 μ l), and chloroform/primary isoamyl alcohol (24:1) (250 μ l) rocks 20 min gently, centrifugal 10 min of 12000 rpm.Get supernatant, repeat above-mentioned steps, until between water and the organic phase without protein layer.Get supernatant, add with volume chloroform/primary isoamyl alcohol, gently shake 20 min, centrifugal 10 min of 12000 rpm.Get supernatant, add 1/10 volume, 3 M NaAc (pH 5.2) and 2 times of cold dehydrated alcohols of volume ,-20 ℃ leave standstill 20 min, centrifugal 20 min of 12500 rpm after shaking up.Nucleic acid is deposited in the pipe end.Abandon supernatant, 70% washing with alcohol precipitation.Collecting precipitation, air drying to ethanol all volatilizees.Add 20 μ l TE (containing RNase A) dissolving DNAs, 37 ℃ leave standstill approximately 30 min after, 4 ℃ of preservations.1% agarose gel electrophoresis detects DNA sample, UV spectrophotometer measuring concentration and purity.
C) site is c.1815C〉the T somatotype is with the design of primer and probe: according to the SNP site c.1815C〉near the T gene order, design HRM somatotype is with primer and probe.Standard is as follows: 1) probe length is 19-35 bp; Only comprise 1 candidate SNP locus, the site occupy in the middle of the probe, does not contain deletion segment; Annealing temperature is 58 ℃-60 ℃; Do not contain simple repeated sequence and palindromic sequence.2) primer length 19-26bp; Do not comprise candidate SNP locus and deletion segment in the primer sequence; Amplified production length 70-130 bp; GC content 30%-70%.Probe TGFBR1pb1 sequence is:
5′-?TTGTAAGGAAGTCGGATACCGAACT?-3′（SEQ?ID?NO:3）；
Primer sequence is upstream primer TGFBR1sf1:
5′-?GAGGTGTCAGACTGATTTTCAGGAG-3′；
Downstream primer TGFBR1sr1:5 '-ATGTCACAAAGGAAATTCATAAAGC-3 '.
D) the SNP site is c.1815C〉the T somatotype: take the genomic dna of 196 individualities of a natural population of chlamys farreri as template, utilize primer TGFBR1sf1 and TGFBR1sr1 to carry out pcr amplification, reaction system is as follows: 10 * Buffer, 1 μ l, 2.5 mM dNTP 0.8 μ l, 2.5 mM MgCl
2, 0.6 μ l, TGFBR1sf1 (10 μ M) 0.1 μ l, TGFBR1sr1 (10 μ M) 0.5 μ l, Taq enzyme (5U/ μ l) 0.1 μ l, template 0.5 μ l, LC Green saturated fluorescence dyestuff 0.7 μ l adds H
2O complements to 10 μ l.Amplified reaction is finished in Biometra T-Gradient PCR system, and reaction conditions is as follows: 94 ℃ of 4 min; 94 ℃ of 40 s, 63 ℃ of 40 s, 60 circulations; 72 ℃ of 5 min.In every part of PCR product, add the corresponding probe TGFBR1pb1 (10 μ M) of 3 μ L, 95 ℃ of sex change 10 min.Get 10 μ l denatured products and change in the 96 hole BLK/WHT plates (Bio-Rad), add 15 μ l mineral oil, centrifugal 1 min of 2000 rpm.Carry out somatotype among the Light-Scanner and detect, be warming up to 95 ℃, the continuous collecting fluorescent signal with the speed of 0.1 ℃/s from 40 ℃.With LightScanner Call IT v2.0 software analysis solubility curve.The genotype of recording individual: the high person of the unimodal temperature of melting curve is identical with probe sequence homozygous, and the bimodal person of melting curve is heterozygous, and the low person of the unimodal temperature of melting curve is homozygous for sudden change.
E) c.1815C〉the heavy correlation analysis of T genotype and closed shell flesh: with each individual closed shell flesh weight of this colony of electronics balance measurement.Utilize One-way ANOVA and post-hoc check, site of analysis is c.1815C〉difference of T different genotype chlamys farreri closed shell flesh weight-average value, check SNP is c.1815C〉dependency of T and chlamys farreri closed shell flesh weight, the result is as shown in the table:
Table 1:c. 1815 C〉T site different genotype chlamys farreri closed shell flesh weight
Closed shell flesh is reused its mean+SD and is represented in the table, if lowercase different expressions in the numerical value upper right corner have significant difference (P＜0.05) in every row.The result shows CC and the individual closed shell flesh of the TT type method of double differences different significantly (p=0.037), the individual closed shell flesh of CT and the TT type method of double differences different significantly (p=0.031).
SNP is c.1815C〉method of the auxiliary high yield chlamys farreri seed selection of T
Because the site is c.1815C〉T is the chlamys farreri of TT type, the weight of its closed shell flesh is significantly higher than CC and the CT type is individual, therefore, in the selection breeding process of high yield chlamys farreri, can carry out the site c.1815C to chlamys farreri breeding candidate colony〉the T somatotype, in conjunction with the somatotype information of other and growth traits related locus, preferentially selecting the site c.1815C〉T is that the individuality of TT type is as breeding parent.Practical application effect shows, by the primer of SNP of the present invention and the parent of probe screening, the weight of closed shell flesh is significantly higher than the not control group of screening among its offspring who breeds.
Claims (5)
1. chlamys farreri TGF-β I receptor gene, its nucleotides sequence is classified SEQ ID NO:1 as.
2. TGF-β I receptor gene claimed in claim 1, the aminoacid sequence of its coding is SEQ ID NO:2.
3. a SNP site relevant with chlamys farreri closed shell flesh weight is characterized in that, described SNP site is 1815 of TGF-β I receptor gene claimed in claim 1, and its base is C or T.
4. SNP claimed in claim 3 site is used for the breeding seed selection of chlamys farreri.
5. for detection of probe and the primer of SNP claimed in claim 3, its sequence information is as follows:
Probe sequence is SEQ ID NO:3;
The upstream primer sequence is SEQ ID NO:4;
The downstream primer sequence is SEQ ID NO:5.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103740729B (en) * | 2014-01-25 | 2015-07-08 | 中国海洋大学 | SNP locus related to growth characteristics of patinopecten yessoensis and detection and application thereof |
| CN103740702B (en) * | 2014-01-07 | 2015-11-18 | 中国科学院海洋研究所 | A kind of SNP marker relevant to bay scallop heat tolerance and authentication method thereof and potential application |
| CN110343742A (en) * | 2019-07-23 | 2019-10-18 | 中国海洋大学 | A kind of micro shellfish DNA extraction method for high-throughput sequencing library preparation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845489A (en) * | 2009-12-03 | 2010-09-29 | 中国海洋大学 | Method for extensively screening scallop SNP |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101845489A (en) * | 2009-12-03 | 2010-09-29 | 中国海洋大学 | Method for extensively screening scallop SNP |
Non-Patent Citations (2)
| Title |
|---|
| 郭慧慧等: "栉孔扇贝myostatin信号通路相关基因的克隆及表达分析", <2008年中国水产学会学术年会论文摘要集> * |
| 郭慧慧等: "栉孔扇贝TGFBR1基因的克隆与表达分析及基因多态性与生长性状的相关性研究", <2011中国遗传学会大会论文摘要汇编> * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103740702B (en) * | 2014-01-07 | 2015-11-18 | 中国科学院海洋研究所 | A kind of SNP marker relevant to bay scallop heat tolerance and authentication method thereof and potential application |
| CN103740729B (en) * | 2014-01-25 | 2015-07-08 | 中国海洋大学 | SNP locus related to growth characteristics of patinopecten yessoensis and detection and application thereof |
| CN110343742A (en) * | 2019-07-23 | 2019-10-18 | 中国海洋大学 | A kind of micro shellfish DNA extraction method for high-throughput sequencing library preparation |
| CN110343742B (en) * | 2019-07-23 | 2023-03-21 | 中国海洋大学 | Trace shellfish DNA extraction method for high-throughput sequencing library preparation |
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