CN102899252A - Method for promoting protein accumulation of spirulina cells - Google Patents
Method for promoting protein accumulation of spirulina cells Download PDFInfo
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- CN102899252A CN102899252A CN2012104188186A CN201210418818A CN102899252A CN 102899252 A CN102899252 A CN 102899252A CN 2012104188186 A CN2012104188186 A CN 2012104188186A CN 201210418818 A CN201210418818 A CN 201210418818A CN 102899252 A CN102899252 A CN 102899252A
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Abstract
The invention provides a method for promoting the protein accumulation of spirulina cells. The method comprises the following steps of filling spirulina liquid with the cell density OD value reaching to 0.9-1.4 in a spirulina tank, enabling the spirulina cells to suspend on the surface of the spirulina tank after standing, placing the spirulina liquid under 600-1800 ummol*m<-2>*s<-1> strong light, irradiating for about 6-8 hours, enabling most of the spirulina cells to sink to the bottom layer of the liquid, and collecting the spirulina cells at the bottom layer and using the spirulina cells as the raw material for extracting the spirulina protein. The protein content of obtained spirulina powder is increased by about more than 40%, the spirulina cultivation efficiency is greatly increased, the production cost is reduced, and the economic benefit is obviously increased. The spirulina powder can be used for the production of the spirulina protein powder, and the spirulina powder has an important value of increasing the quality of a spirulina product and developing the spirulina protein resource.
Description
Technical field
The present invention relates to improve the method for plant plankton protein content, specifically, relate to the method that improves the spirulina cells protein content.
Background technology
Spirulina (Spirulina) has another name called blue-green algae, have the effects such as nourishing, strong, replenishing qi and blood, anti-malnutrition, it is rich in abundant VITAMIN, protein, unsaturated fatty acids, trace element, especially its protein of containing is the Major Nutrient material of spirulina, and spirulina has been widely used in the aspects such as medicines and health protection, food interpolation, feed interpolation, aquaculture, fine chemistry industry.Has multiple biological activity such as the Phycocyanins, C-in the hydrolysis of protein spirulina, Phycocyanins, C-has the immunity of organisms of raising and antitumor action, anti-oxidant and antidotal effect, antifatigue and antiviral effect, the multiple pharmacological effect such as effect of radioprotective and anti-mutation have widely purposes in medicines and health protection and genetically engineered field.
In recent decades, both at home and abroad to spirulina cells protein separation, extraction and applied research, or become polypeptide to be used for healthcare products and food the hydrolysis of protein spirulina enzymolysis to add.General bibliographical information, spirulina cells protein content 60-70%, however be 50-60% at the protein content that most of product is produced in the spirulina enterprise scale.Hydrolysis of protein spirulina content is one of Spirulina Products important indicator, food spiral algae powder national standard protein content 〉=55%.As seen, improve hydrolysis of protein spirulina content to the quality that improves Spirulina Products, develop new product and the spirulina protein resource utilize significant.
Summary of the invention
The present invention is intended to provide a kind of method that improves the spirulina cells protein content for the low defective of spirulina cells protein content in producing, and the method comprises the steps: 1) cultivate spirulina cells, reach OD value 0.9-1.4 to the cell density of algae liquid; The spirulina cells that 2) will be suspended in the algae pool surface is exposed to high light 600-1800 μ molm
-2S
-1Lower, shone 6 ~ 8 hours, collect the spirulina cells of sinking.
Aforesaid method comprises that also the spirulina cells that makes sinking placed under the dark condition 10 ~ 16 hours, collected the step of the spirulina cells of come-up.
Wherein, the nutrient solution that the cultivation spirulina cells uses in the step 1) is the Zarrouk nutrient solution, and it is as follows to fill a prescription: sodium bicarbonate 16.8g/L, dipotassium hydrogen phosphate 0.5g/L, SODIUMNITRATE 2.4g/L, vitriolate of tartar 1.0g/L, sodium-chlor 1.0g/L, seven water and sal epsom 0.2g/L, two water and calcium chloride 0.04g/L, ferrous sulfate 0.2g/L, EDETATE SODIUM 0.08g/L, pH value 8.25 ~ 10 is prepared with water.
Preferably, the carbon-nitrogen ratio of algae liquid is controlled at 4.3 ~ 6.1 in the step 1).
Preferably, the algae tank depth surpasses 30cm step 2).Leave standstill and make most of frustule float on the liquid level layer more than 3 ~ 6 hours.
Preferably, step 2) the algae liquid temp is controlled at 35 ~ 47 ℃ in, to promote that frustule sinks fast.
The present invention also provides by the spirulina cells of aforesaid method preparation and food and the healthcare products that prepared by described spirulina cells.Also can be made into the high algae mud of protein content and dry algae powder product.
Compared with prior art, the invention has the advantages that:
The present invention does not have particular requirement for the preculture liquid of spirulina algae kind and employing, and use range is more extensive; Under certain condition, use strong illumination and relatively-high temperature, cooperate lower carbon-nitrogen ratio, it is about more than 40 percentage points that spirulina protein content is increased, the method is workable, and effect is remarkable, has greatly reduced the production cost of spirulina protein, remarkable in economical benefits, and widened the spirulina protein range of application.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to scope of the present invention.
If do not specialize, the conventional means that used technique means is well known to those skilled in the art among the embodiment.
The Zarrouk nutrient solution that adopts in following examples, it is as follows to fill a prescription: sodium bicarbonate 16.8g/L, dipotassium hydrogen phosphate 0.5g/L, SODIUMNITRATE 2.4g/L, vitriolate of tartar 1.0g/L, sodium-chlor 1.0g/L, seven water and sal epsom 0.2g/L, two water and calcium chloride 0.04g/L, ferrous sulfate 0.2g/L, EDETATE SODIUM 0.08g/L, pH value 8.25 ~ 10 is prepared with water.
Embodiment 1 improves the method for spirulina cells protein content
Operation steps is as follows:
1) spirulina algae kind is inoculated in the Zarrouk nutrient solution, inoculum density is OD value 0.3,
At 30 ~ 35 ℃, cultivated under the culture condition of pH value 8.25 ~ 10.0 5 ~ 7 days, make it reach logarithmic phase, and make that spirulina cells density reaches OD value 0.9 ~ 1.4 in the algae liquid;
2) carbon-nitrogen ratio with step 1) algae liquid is controlled at 6.1, injects 10 liters glass jar or algae pond (the algae liquid degree of depth is 35cm), leaves standstill 3 hours, and temperature regulation to 35 ℃ is exposed to high light 600 μ molm
-2S
-1Lower, shone 6 hours, after most of frustule sinks, the spirulina cells that is sunken to bottom was placed dark condition lower 10 hours, make its most cells come-up, the spirulina cells of collecting come-up is used for extracting hydrolysis of protein spirulina, and gained spirulina powder protein content can reach 71.2%.
Embodiment 2 improves the method for spirulina cells protein content
Operation steps is as follows:
1) spirulina algae kind is inoculated in the Zarrouk nutrient solution, inoculum density is OD value 0.3,
At 30 ~ 35 ℃, cultivated under the culture condition of pH value 8.25 ~ 10.0 5 ~ 7 days, make it reach logarithmic phase, and make that spirulina cells density reaches OD value 0.9 ~ 1.4 in the algae liquid;
2) carbon-nitrogen ratio with step 1) algae liquid is controlled at 5.3, and spirulina liquor injects 10 liters glass jar or algae pond (the algae liquid degree of depth is 42cm), leaves standstill 5 hours, and temperature regulation to 43 ℃ is exposed to high light 1300 μ molm
-2S
-1Lower, shone 7 hours, after most of frustule sinks, the spirulina cells that is sunken to bottom was placed dark condition lower 14 hours, make its most cells come-up, the spirulina cells of collecting come-up is used for extracting hydrolysis of protein spirulina, and gained spirulina powder protein content can reach 82.1%.
Embodiment 3 improves the method for spirulina cells protein content
Operation steps is as follows:
1) spirulina algae kind is inoculated in the Zarrouk nutrient solution, inoculum density is OD value 0.3,
At 30 ~ 35 ℃, cultivated under the culture condition of pH value 8.25 ~ 10.0 5 ~ 7 days, make it reach logarithmic phase, and make that spirulina cells density reaches OD value 0.9 ~ 1.4 in the algae liquid;
2) carbon-nitrogen ratio with step 1) algae liquid is controlled at 4.3, and spirulina liquor injects 10 liters glass jar or algae pond (the algae liquid degree of depth is 50cm), leaves standstill 6 hours, and temperature regulation to 47 ℃ is exposed to high light 1800 μ molm
-2S
-1Lower, shone 8 hours, after most of frustule sinks, the spirulina cells that is sunken to bottom was placed dark condition lower 16 hours, make its most cells come-up, the spirulina cells of collecting come-up is used for extracting hydrolysis of protein spirulina, and gained spirulina powder protein content can reach 75.3%.
The cultural method (contrast) of embodiment 4 spirulina cells
Operation steps is as follows:
1) spirulina algae kind is inoculated in the Zarrouk nutrient solution, inoculum density is OD value 0.3,
At 30 ~ 35 ℃, cultivated under the culture condition of pH value 8.25 ~ 10.0 5 ~ 7 days, make it reach logarithmic phase, and make that spirulina cells density reaches OD value 0.9 ~ 1.4 in the algae liquid;
2) carbon-nitrogen ratio with step 1) algae liquid is controlled at 6.0, and spirulina liquor injects 10 liters glass jar or algae pond (the algae liquid degree of depth is 40cm), leaves standstill 3 ~ 6 hours, and temperature regulation to 32 ℃ is 200 ~ 500 μ molm in illumination
-2S
-1Lower, shone 6 ~ 8 hours, the gathering screw frustule is used for extracting hydrolysis of protein spirulina, hydrolysis of protein spirulina content 50.0%.
By embodiment 1 ~ 3 is compared with the cultivation results of embodiment 4, can find out that adopt spirulina breeding method of the present invention, it is about more than 40 percentage points that spirulina protein content is increased.Greatly improve spirulina breeding efficient, reduced production cost, significantly improved economic benefit, widened the spirulina protein range of application.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a method that improves the spirulina cells protein content is characterized in that, described method comprises the steps:
1) cultivates spirulina cells, reach OD value 0.9-1.4 to the cell density of algae liquid;
The spirulina cells that 2) will be suspended in the algae pool surface is exposed to high light 600-1800 μ molm
-2S
-1Lower, shone 6 ~ 8 hours, collect the spirulina cells of sinking.
2. method according to claim 1 is characterized in that, comprises that also the spirulina cells that makes sinking placed under the dark condition 10 ~ 16 hours, collected the step of the spirulina cells of come-up.
3. method according to claim 1 and 2 is characterized in that, the nutrient solution that the cultivation spirulina cells uses in the step 1) is the Zarrouk nutrient solution, it is as follows to fill a prescription: sodium bicarbonate 16.8g/L, dipotassium hydrogen phosphate 0.5g/L, SODIUMNITRATE 2.4g/L, vitriolate of tartar 1.0g/L, sodium-chlor 1.0g/L, seven water and sal epsom 0.2g/L, two water and calcium chloride 0.04g/L, ferrous sulfate 0.2g/L, EDETATE SODIUM 0.08g/L, pH value 8.25 ~ 10 is prepared with water.
4. method according to claim 1 and 2 is characterized in that, the carbon-nitrogen ratio of algae liquid is controlled at 4.3 ~ 6.1 in the step 1).
5. method according to claim 1 and 2 is characterized in that step 2) in the algae tank depth surpass 30cm.
6. method according to claim 1 and 2 is characterized in that step 2) in the algae liquid temp be controlled at 35 ~ 47 ℃.
7. the spirulina cells that is prepared by each described method of claim 1 ~ 6.
8. the food and the healthcare products that are prepared by the described spirulina cells of claim 7.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108913635A (en) * | 2018-08-10 | 2018-11-30 | 中国科学院青岛生物能源与过程研究所 | A method of producing recoverin matter content during glycosylglycerol |
| CN108949644A (en) * | 2018-09-19 | 2018-12-07 | 中国科学院青岛生物能源与过程研究所 | A method of producing recoverin matter content during glycosylglycerol |
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| CN101748067A (en) * | 2008-12-19 | 2010-06-23 | 李博生 | Method for promoting polysaccharide accumulation of spirulina cells |
| CN102517244A (en) * | 2011-12-31 | 2012-06-27 | 北京林业大学 | Method for improving metallothionein content of spirulina |
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| CN101748067A (en) * | 2008-12-19 | 2010-06-23 | 李博生 | Method for promoting polysaccharide accumulation of spirulina cells |
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Cited By (2)
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| CN108913635A (en) * | 2018-08-10 | 2018-11-30 | 中国科学院青岛生物能源与过程研究所 | A method of producing recoverin matter content during glycosylglycerol |
| CN108949644A (en) * | 2018-09-19 | 2018-12-07 | 中国科学院青岛生物能源与过程研究所 | A method of producing recoverin matter content during glycosylglycerol |
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