CN102812929A - Artificial induced spawning of Phascolosoma esculenta - Google Patents

Abstract

The invention discloses a method for artificially inducing labor of D. pallidum. By selecting female and male parents with relatively high maturity of egg cells and sperm, first drying them in the shade for 20-30 hours in a bamboo curtain, and then placing them in a net basket with an internal surface flow rate of 0.2- The water in the 0.3m/s oxytocin pool was stimulated for 58-66h, and the distance between the bottom of the net basket and the surface of the water body in the oxytocin pool was 5-8cm. The bamboo curtain was used to stimulate the body to dry in the shade and the effect was good, which was conducive to oxytocin. Stimulated by concentrated running water in the net basket, the rate of induced labor and insemination is high. Disodium edetate can chelate heavy metal ions in seawater and avoid the adverse effects of heavy metal ions on insemination and embryonic development. Stimulated by running water in the net basket, more sperm and egg cells are produced, the fertilization rate is over 90%, and an average of 5,895,500 fertilized eggs are obtained per kg of brood.

Description

The method of artificially inducing labor of Derma pallidum

technical field

The present invention relates to the technology of oxytocin for animals, in particular to the artificial oxytocin method of D. palasis.

Background technique

Phascolosoma esculenta (Latin Phascolosoma esculenta ) belongs to the genus Phascolosoma esculenta, commonly known as Haiding. It is distributed in the southeast coast and lives in caves in the intertidal zone. Cocoa leather capsule has high nutritional, economic and medicinal value, and is known as the "Cordyceps sinensis" in the sea. At present, the coastal areas of Zhejiang, Fujian, and Guangdong provinces are developing the characteristic aquaculture industry of D. coli, which is dioecious, and the sperm and eggs are discharged into the water for fertilization. The seedlings for breeding mainly rely on wild seedlings. Although Wu Hongxi et al. disclosed the "Artificial Propagation Test of Dermatophora coli" in "Marine Science" (March, 2010, 22-25), the artificial propagation test was to naturally air-dry the broodstock in a cool and ventilated place for 10 days in the laboratory. -20 h or cooling down to 8-10 ℃ for 24-36 h, placed in a small water body (cylindrical tank with a height of 0.85 m and a diameter of 1.7 m) to induce labor, 45.5 kg of broodstock were shared in 7 experiments, and the total number of fertilized eggs obtained was 35.27 million eggs, the average number of fertilized eggs obtained per kg parent body was 775,200 eggs. However, this artificial propagation test is only a preliminary exploration in a small water body in the laboratory. The amount of fertilized eggs obtained per kg of broodstock is low, and the artificial reproduction rate is low.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method for artificially inducing labor of D. pallidum with a large number of fertilized eggs obtained per kg of parent body and a high artificial reproduction rate.

The technical solution adopted by the present invention to solve the problems of the technologies described above is: the method for artificially inducing labor of D. coulis, the steps of which are as follows:

a. Broodstock selection: During the breeding season, select broodstock with complete insect bodies, strong vitality, flexible snout, light brown body surface, no disability, and an individual weight of 2-4 g. The broodstock is composed of females with a quantitative ratio of 1:1. Composed of broodstock and male broodstock, the minimum and maximum diameters of more than 80% oval-shaped egg cells in the body cavity of the female broodstock are not less than 110-120 mm×140-150 mm, and more than 80% of the sperm in the body cavity of the male broodstock The minimum diameter and the maximum diameter are not less than 20-40mm × 20-50mm; the egg cells and sperms with higher maturity can be selected to induce labor successfully;

b. Drying stimulation in the shade: place 10-15 kg of the body in a bamboo curtain, and dry it in the shade indoors for 20-30 hours. The gap between the bamboo strips is not more than 0.1 mm; the bamboo curtain can make the body dry evenly in the shade, and the effect is good. conducive to labor induction;

c. Flowing water stimulation: Soak and disinfect the above-mentioned body dried in the shade with potassium permanganate solution with a concentration of 15-20 mg/l for 10-15 minutes, then rinse with filtered seawater for 3-5 minutes, and then pour it into a net basket. The length of the net basket is 0.8-1.2m, the width is 0.6-0.8m, and the height is 0.15-0.2m. The mesh of the net basket is 30-40 mesh. The net basket is immersed in the oxytocin pool, and the oxytocin The temperature of the water body in the pool is 28-31 ℃, pH 7.9-8.3, and salinity 18-25‰, and 4-5g of disodium ethylenediaminetetraacetic acid is added to each cubic meter of water in the oxytocin pool, and the water body in the oxytocin pool is deep is 1.2-1.3 m, the distance between the bottom of the net basket and the surface of the water body in the oxytocin pool is 5-8 cm, the flow velocity of the water body surface layer in the oxytocin pool is 0.2-0.3 m/s, and the parent body is stimulated by flowing water in the oxytocin pool. After 58-66h, the parent body was removed after being stimulated by running water. Fertilize the egg cell with the sperm to form a fertilized egg.

Compared with the prior art, the advantage of the present invention is that the method for artificially inducing labor of D. coli, by selecting the female and male parents with higher maturity of egg cells and sperm, first drying and stimulating in the bamboo curtain for 20-30 h, and then Placed in a net basket in an oxytocin pool with a surface velocity of 0.2-0.3 m/s and stimulated for 58-66 hours, the distance between the bottom of the net basket and the surface of the water body in the oxytocin pool was 5-8 cm. Uniform drying in the shade, good effect, is conducive to induced labor, stimulated with concentrated running water in a mesh basket, the induced labor rate and insemination rate are high, disodium edetate can chelate heavy metal ions in seawater, and avoid heavy metal ions from affecting fertilization and embryonic development Unfavorable effects, so the parent body is stimulated by drying in the shade in the bamboo curtain and running water in the net basket, more sperm and egg cells are produced, the fertilization rate is over 90%, and an average of 5,895,500 fertilized eggs are obtained per kg of the parent body.

Detailed ways

Below in conjunction with embodiment the present invention is described in further detail.

Example 1

The method of artificial oxytocin of D. coli, in August of the breeding season, select broodstock with complete body, strong vitality, flexible snout, light brown body surface, no disability, individual weight 2-4 g, among which the female broodstock The ratio of the number of male broodstock to that of male broodstock is 1:1, the size of more than 80% oval oocytes in the body cavity of female broodstock is not less than 110 -120 mm×140 -150 mm, and the size of 80% of sperm in the body cavity of male broodstock is not less than 20- 40mm × 20-50mm; put 15 kg body in a bamboo curtain, and dry it indoors for 24 hours. The bamboo curtain is 2 m long, 1 m wide, the edge is vertically bent upwards by 5 cm, and the gap between the bamboo strips is not greater than 0.1 mm; After drying in the shade, sterilize by soaking in potassium permanganate solution with a concentration of 15-20 mg/L for 10-15 minutes, then rinse with filtered seawater for 3-5 minutes, and then pour the dried parents into a length of 0.8 -1.2m , with a width of 0.6-0.8 m, a height of 0.15-0.2 m, and a mesh of 30-40 meshes in a net basket, immerse the mesh stimulation basket in the oxytocin pool, the water temperature in the oxytocin pool is 28 -31 ℃, pH 7.9- 8.3. The salinity is 18-25. Add 4-5g of disodium edetate to each cubic meter of water in the oxytocin pool. The water depth of the oxytocin pool is 1.2 -1.3 m. The distance between the bottom of the net basket and the water surface of the oxytocin pool is 5-8 cm, the flow velocity of the surface of the water body in the oxytocin pool is 0.2-0.3 m/s, the body is stimulated by flowing water in the oxytocin pool for 60 hours, and the body is removed after the water stimulation, and the sperm and egg cells are fertilized into fertilized eggs; 72 million fertilized eggs are obtained , the fertilization rate is about 93%.

Example 2

It is basically the same as Example 1, except that the parent body is 14 kg, the stimulation time of drying in the shade is 21 hours, and the stimulation time of running water is 66 hours, and 86.5 million fertilized eggs are obtained, and the fertilization rate is about 95%.

Example 3

It is basically the same as in Example 1, except that the parent body is 10 kg, the stimulation time of drying in the shade is 30 hours, and the stimulation time of running water is 58 hours, and 55 million fertilized eggs are obtained, and the fertilization rate is about 92%.

Example 4

It is basically the same as Example 1, except that the parent body is 10 kg, the stimulation time of drying in the shade is 26 hours, and the stimulation time of running water is 62 hours, and 67.5 million fertilized eggs are obtained, and the fertilization rate is about 95%.

Claims (1)

1. the method for artificially inducing labor of Derma spp. is characterized in that the steps are as follows: a. Broodstock selection: During the breeding season, select broodstock with complete insect bodies, strong vitality, flexible snout, light brown body surface, no disability, and an individual weight of 2-4 g. The broodstock is composed of females with a quantitative ratio of 1:1. Composed of broodstock and male broodstock, the minimum and maximum diameters of more than 80% oval-shaped egg cells in the body cavity of the female broodstock are not less than 110-120 mm×140-150 mm, and more than 80% of the sperm in the body cavity of the male broodstock The minimum and maximum diameters are not less than 20-40mm × 20-50mm; b. Drying stimulation in the shade: place 10-15 kg of the body in a bamboo curtain, and dry it in the shade indoors for 20-30 hours. The gap between bamboo strips is not more than 0.1 mm; c. Flowing water stimulation: Soak and disinfect the above-mentioned body dried in the shade with potassium permanganate solution with a concentration of 15-20 mg/l for 10-15 minutes, then rinse with filtered seawater for 3-5 minutes, and then pour it into a net basket. The length of the net basket is 0.8-1.2m, the width is 0.6-0.8m, and the height is 0.15-0.2m. The mesh of the net basket is 30-40 mesh. The net basket is immersed in the oxytocin pool, and the oxytocin The temperature of the water body in the pool is 28-31 ℃, pH 7.9-8.3, and salinity 18-25‰, and 4-5g of disodium ethylenediaminetetraacetic acid is added to each cubic meter of water in the oxytocin pool, and the water body in the oxytocin pool is deep is 1.2-1.3 m, the distance between the bottom of the net basket and the surface of the water body in the oxytocin pool is 5-8 cm, the flow velocity of the water body surface layer in the oxytocin pool is 0.2-0.3 m/s, and the parent body is in the The running water in the oxytocin pool was stimulated for 58-66 hours, and the parent body was removed after the running water stimulated.