CN102783583A - Complex immunoenhancer for low salinity prawn as well as preparation method and application for composite immunopotentiator - Google Patents
Complex immunoenhancer for low salinity prawn as well as preparation method and application for composite immunopotentiator Download PDFInfo
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- CN102783583A CN102783583A CN2012103084118A CN201210308411A CN102783583A CN 102783583 A CN102783583 A CN 102783583A CN 2012103084118 A CN2012103084118 A CN 2012103084118A CN 201210308411 A CN201210308411 A CN 201210308411A CN 102783583 A CN102783583 A CN 102783583A
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Abstract
The invention relates to a composite immunopotentiator for low salinity prawn as well as a preparation method and an application for the composite immunopotentiator The composite immunopotentiator is characterized by comprising the following components by weight percentage: 1-2kg of magnesium sulfate, 1-2kg of bacillus subtilis, 1-2kg of bacillus Licheniformis, 1-2kg of lactic acid bacillus, 1-2kg of mannan oligosaccharide and 2-3kg of fructo-oligosaccharide. The preparation method comprises the steps of: weighing the magnesium sulfate, the bacillus subtilis, the bacillus Licheniformis, the lactic acid bacillus, the mannan oligosaccharide and the fructo-oligosaccharide in proportion, and grinding into fine powder; then mixing uniformly in a mixer, wherein a coefficient of variation in a mixing process in the mixer is smaller than or equal to 5%; and forming the composite immunopotentiator for low salinity prawn. The application of the composite immunopotentiator for the low salinity prawn is that: the composite immunopotentiator for the low salinity prawn is used in a feed for the prawn as a feed additive, and the composite immunopotentiator for the low salinity prawn is added into the feed for the prawn to form a composite immune feed for the prawn. The composite immunopotentiator for the low salinity prawn can remarkably increase a non-characteristic immunity of body of the low salinity prawn and enhance a resistance to diseases.
Description
Technical field
The present invention relates to a kind of low salinity prawn with composite immune reinforcing agent, be applicable to low salinity Penaeus Vannmei prevention of damage by disease.Belong to technical field of aquaculture feedstuff.
Background technology
Allly receive prawn (Litopenaeus vannamei), be commonly called as Penaeus Vannmei, originate in the eurysalinity shell-fish in America, adapt to salinity range (0.5 1 40 ‰).In recent years, coastal seawater pollutes and reason such as market demand increase, and the breed of Environment of Litopenaeus vannamei Low has significantly advanced in the low salinity water territory from littoral body of sea water upcountry, particularly in the Delta of the Pearl River, Yangtze River Delta, the Huanghai Sea and the Bohai Sea Area of China.Statistics in 2009 shows that China hangs down salinity (0.5-5 ‰) breed (also being freshwater aquiculture) all output of receiving prawn and accounts for 40% of total amount.Along with freshwater aquiculture intensification degree improves, breeding environment worsens day by day, the generation of prawn disease, and the extent of injury is serious day by day.
In actual production, generally adopt antibiotic and other drug to prevent the generation of prawn disease.Because problems such as medicament residue and bacterial drug resistances, the cry that limits the antibiotic use in the world is increasingly high.At present, the someone repays examination and in feed, improves the immunity of aquatic livestock through adding immunopotentiator, to reduce antibiotic use, can receive certain effect.But the immunopotentiator of existing single component is not only to the generation poor effect of prevention prawn disease, and on culturing long-term the use toward contact meeting appearance of negative feedback inhibition problem.Therefore, form to the inorganic ions of less salt water body and the variation characteristics of prawn metabolic mechanism are developed the feed addictive that promotes the low salinity environment of prawn to culture growth rate and survival rate down and just seemed particularly important.Yet, still be not directed against the immunopotentiator product under the Environment of Litopenaeus vannamei Low low-salt environment at present on the domestic and international market.
Summary of the invention
One of the object of the invention; Be for the immunopotentiator that overcomes existing single component not only to the shortcoming of the generation poor effect of prevention prawn disease; Provide a kind of low salinity prawn to use composite immune reinforcing agent, it can promote prawn nospecific immunity in hypotonic environment, improves the premunition of prawn.
Two of the object of the invention is for the preparation method of a kind of low salinity prawn with composite immune reinforcing agent is provided.
Three of the object of the invention is for the purposes of a kind of low salinity prawn with composite immune reinforcing agent is provided.
One of the object of the invention can reach through taking following technical scheme:
A kind of prawn is used composite immune reinforcing agent, it is characterized in that: said composite immune reinforcing agent is made up of magnesium sulfate, withered grass brood cell bacterium, lichens brood cell bacterium, lactic acid brood cell bacterium, manna oligosacchride and FOS; Addition with feed per ton is a radix, and the addition of said composite immune reinforcing agent is 7-13kg; In said composite immune reinforcing agent, the weight percentage of each material component is respectively magnesium sulfate 1-2kg, withered grass brood cell bacterium 1-2kg, lichens brood cell bacterium 1-2kg, lactic acid brood cell bacterium 1-2kg, manna oligosacchride 1-2kg and FOS 2-3kg.
One of the object of the invention can also reach through taking following technical scheme:
A kind of Technological improvement plan of realizing one of the object of the invention is: the concentration of withered grass brood cell bacterium is 10-20 hundred million CFU/g, and the concentration of lichens brood cell bacterium is 5-10 hundred million CFU/g, and the concentration of lactic acid brood cell bacterium is 5-10 hundred million CFU/g.
Two of the object of the invention can reach through taking following technical scheme:
A kind of low salinity prawn is with the preparation method of composite immune reinforcing agent; It is characterized in that: take by weighing magnesium sulfate, withered grass brood cell bacterium, lichens brood cell bacterium, lactic acid brood cell bacterium, manna oligosacchride and FOS closely in proportion; These six kinds of raw materials are worn into fine powder; In mixer, mix then, in mixer, require the coefficient of variation≤5% in the mixed process, constitute compound low salinity prawn and use immunopotentiator.
Three of the object of the invention can reach through taking following technical scheme:
A kind of low salinity prawn is with the purposes of composite immune reinforcing agent; It is characterized in that: in prawn feed, use as feed addictive; To hang down the salinity prawn and be added into formation complex immunity prawn feed in the prawn feed with composite immune reinforcing agent, said low salinity prawn is used the 0.1-0.2% of the addition of composite immune reinforcing agent as the complex immunity prawn feed.
The present invention has following advantage:
1, adopts low salinity prawn of the present invention to use composite immune reinforcing agent, can obviously strengthen the non-characteristic immunity of the body of low salinity prawn, improve survival rate, strengthen the resistance that vibrios, white spot virus, Taura syndrome etc. is caused disease.
2, to utilize probio and oligosaccharides be synergy in body in the present invention, and biological value is high, significantly improves the digestibility of prawn, promotes growth, lowers feed coefficient.
3, the present invention for the palatability of feed do not have any harmful effect, long-term safe in utilization efficient, have no side effect.
4, its raw material source of the present invention is stable, cheap, and production technology is simple.
The specific embodiment
The low salinity prawn that the present invention relates to is used composite immune reinforcing agent, is made up of magnesium sulfate, withered grass brood cell bacterium, lichens brood cell bacterium, lactic acid brood cell bacterium, manna oligosacchride and FOS; Addition with feed per ton is a radix, and the addition of said composite immune reinforcing agent is 7-13kg; In said composite immune reinforcing agent, the weight percentage of each material component is respectively magnesium sulfate 1-2kg, withered grass brood cell bacterium 1-2kg, lichens brood cell bacterium 1-2kg, lactic acid brood cell bacterium 1-2kg, manna oligosacchride 1-2kg and FOS 2-3kg.Specific embodiment is described below:
Specific embodiment 1:
Take by weighing: magnesium sulfate 1kg, withered grass brood cell bacterium 1kg; Lichens brood cell bacterium 1kg, lactic acid brood cell bacterium 1kg, manna oligosacchride 1kg, FOS 2kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 7kg.
In the present embodiment, the concentration of withered grass brood cell bacterium is 10-20 hundred million CFU/g, and the concentration of lichens brood cell bacterium is 5-10 hundred million CFU/g, and the concentration of lactic acid brood cell bacterium is 5-10 hundred million CFU/g.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 7kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.07% of complex immunity prawn feed.
Experiment shows, uses this composite immune reinforcing agent in the complete period that salinity (1-5 ‰) Penaeus Vannmei is cultured, and survival rate improves 20-30% respectively.
Specific embodiment 2:
Take by weighing: magnesium sulfate 2kg, withered grass brood cell bacterium 2kg; Lichens brood cell bacterium 2kg, lactic acid brood cell bacterium 2kg, manna oligosacchride 2kg, FOS 3kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 13kg.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 13kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.13% of complex immunity prawn feed.
Specific embodiment 3:
Take by weighing: magnesium sulfate 1.5kg, withered grass brood cell bacterium 1.5kg; Lichens brood cell bacterium 1.5kg, lactic acid brood cell bacterium 1.5kg, manna oligosacchride 1.5kg, FOS 2.5kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 10kg.
Experiment shows, uses this composite immune reinforcing agent in the complete period that salinity (1-5 ‰) Penaeus Vannmei is cultured, and survival rate improves 20-30% respectively.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 10kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.10% of complex immunity prawn feed.
Specific embodiment 4:
Take by weighing: magnesium sulfate 1.8kg, withered grass brood cell bacterium 1.8kg; Lichens brood cell bacterium 1.8kg, lactic acid brood cell bacterium 1.8kg, manna oligosacchride 1.8kg, FOS 2.7kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 11.7kg.
Experiment shows, uses this composite immune reinforcing agent in the complete period that salinity (1-5 ‰) Penaeus Vannmei is cultured, and survival rate improves 20-30% respectively.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 11.7kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.17% of complex immunity prawn feed.
Specific embodiment 5:
Take by weighing: magnesium sulfate 1.2kg, withered grass brood cell bacterium 1.6kg; Lichens brood cell bacterium 1.6kg, lactic acid brood cell bacterium 1.2kg, manna oligosacchride 1.2kg, FOS 2.2kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 9kg.
Experiment shows, uses this composite immune reinforcing agent in the complete period that salinity (1-5 ‰) Penaeus Vannmei is cultured, and survival rate improves 20-30% respectively.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 9kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.09% of complex immunity prawn feed.
Specific embodiment 6:
Take by weighing: magnesium sulfate 1.6kg, withered grass brood cell bacterium 1.2kg; Lichens brood cell bacterium 1.2kg, lactic acid brood cell bacterium 1.7kg, manna oligosacchride 1.3kg, FOS 2.6kg, these six kinds of raw materials are worn into fine powder; In mixer, mix (requiring the coefficient of variation≤5%) then, promptly process compound low salinity prawn and use immunopotentiator 9.6kg.
During use; Use in prawn feed as feed addictive; To process compound low salinity prawn with constituting the complex immunity prawn feed in the prawn feed of immunopotentiator 9.6kg adding 1 ton (1000kg), said low salinity prawn uses the addition of composite immune reinforcing agent to be 0.096% of complex immunity prawn feed.
Experiment shows, uses this composite immune reinforcing agent in the complete period that salinity (1-5 ‰) Penaeus Vannmei is cultured, and survival rate improves 20-30% respectively.
The prawn of a kind of less salt provided by the invention is immunity, physilogical characteristics to the prawn body with immunopotentiator, selects to add magnesium sulfate, and its purpose can strengthen the GAP-associated protein GAP enzyme of prawn body lives, and resists the environment volume hypotonic stress that comes from the outside.Select for use Bacillus subtillis, bacillus licheniformis and brood cell lactic acid bacteria to can be used as good immunoactivator, the humoral immunity of excitating organism and cellular immunity are come the enhancing body immunity function.Its mechanism of action possibly be to stimulate the nospecific immunity system that it is played a role through bacterium itself or cell wall constituent; Thereby raising animal immunizing power; Multiplely in crustacean, be confirmed as immunopotentiator from the bacteria cell wall composition; And its metabolin can improve the ability of digesting and assimilating of prawn, thereby improves efficiency of feed utilization.Oligosaccharides has certain immunogenicity; Can stimulate immune response, and can combine with some toxin, virus and fungal cell's surface and as the adjuvant of these exogenous antigens, the absorption that slows down antigen; Increase tiring of antigen, thereby strengthen the cell and the humoral immune reaction of animal body.Add oligosaccharides, probio and multi-form compatibility and can to a certain degree increase prawn intestinal bifidobacteria quantity, significantly reduce enteron aisle vibrios number.Oligosaccharides can suppress the growth of kinds of pathogenic vibrio significantly and improve the propagation of useful Bifidobacterium.Its possible reason is that oligosaccharides can promote beneficial microbe to breed in a large number as the MF of probio, regulates microecological balance.And oligosaccharides class material can combine with the lectin specificity of pathogen through the competitiveness to the intestinal mucosa epithelium, and pathogen can not be adhered on the intestines wall, and gets rid of external with oligosaccharides.
The immunopotentiator that the present invention relates to has promotion or brings out host defense; Strengthen one type of material of living organism resistance against diseases; Act on the nospecific immunity system of aquatic invertebrate, thereby the immunity function that activates body self reaches protection effect, strengthens its premunition.Compare advantage such as immunopotentiator has safety, noresidue, sphere of action is wide and easy to use with vaccine with antibiotic.
Claims (4)
1. a prawn is used composite immune reinforcing agent, it is characterized in that: said composite immune reinforcing agent is made up of magnesium sulfate, withered grass brood cell bacterium, lichens brood cell bacterium, lactic acid brood cell bacterium, manna oligosacchride and FOS; Addition with feed per ton is a radix, and the addition of said composite immune reinforcing agent is 7-13kg; In said composite immune reinforcing agent, the weight percentage of each material component is respectively magnesium sulfate 1-2kg, withered grass brood cell bacterium 1-2kg, lichens brood cell bacterium 1-2kg, lactic acid brood cell bacterium 1-2kg, manna oligosacchride 1-2kg and FOS 2-3kg.
2. a kind of prawn according to claim 1 is used composite immune reinforcing agent, it is characterized in that: the concentration of withered grass brood cell bacterium is 10-20 hundred million CFU/g, and the concentration of lichens brood cell bacterium is 5-10 hundred million CFU/g, and the concentration of lactic acid brood cell bacterium is 5-10 hundred million CFU/g.
3. a kind of low salinity prawn as claimed in claim 1 is with the preparation method of composite immune reinforcing agent; It is characterized in that: take by weighing magnesium sulfate, withered grass brood cell bacterium, lichens brood cell bacterium, lactic acid brood cell bacterium, manna oligosacchride and FOS closely in proportion; These six kinds of raw materials are worn into fine powder; In mixer, mix then, in mixer, require the coefficient of variation≤5% in the mixed process, constitute compound low salinity prawn and use immunopotentiator.
4. a kind of low salinity prawn as claimed in claim 1 is with the purposes of composite immune reinforcing agent; It is characterized in that: in prawn feed, use as feed addictive; To hang down the salinity prawn and be added into formation complex immunity prawn feed in the prawn feed with composite immune reinforcing agent, said low salinity prawn is used the 0.1-0.2% of the addition of composite immune reinforcing agent as the complex immunity prawn feed.
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Citations (3)
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|---|---|---|---|---|
| CN1620892A (en) * | 2004-12-10 | 2005-06-01 | 中国海洋大学 | Compound microecological preparation for prawn |
| CN101054550A (en) * | 2007-04-10 | 2007-10-17 | 天津生机集团有限公司 | Probiotics composition capable of accelerating growth of freshwater fish |
| CN101099541A (en) * | 2006-11-28 | 2008-01-09 | 湛江粤海饲料有限公司 | Low-salinity cultivating litopenaeus vannamei additive premixture |
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- 2012-08-25 CN CN2012103084118A patent/CN102783583A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1620892A (en) * | 2004-12-10 | 2005-06-01 | 中国海洋大学 | Compound microecological preparation for prawn |
| CN101099541A (en) * | 2006-11-28 | 2008-01-09 | 湛江粤海饲料有限公司 | Low-salinity cultivating litopenaeus vannamei additive premixture |
| CN101054550A (en) * | 2007-04-10 | 2007-10-17 | 天津生机集团有限公司 | Probiotics composition capable of accelerating growth of freshwater fish |
Non-Patent Citations (5)
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| 《中国博士学位论文全文数据库 农业科技辑》 20080315 胡毅 凡纳滨对虾饲料配方优化及几种饲料添加剂的应用 , 第3期 * |
| 《中山大学学报(自然科学版)》 20080731 朱学芝等 微生态制剂对凡纳滨对虾生长及水质的影响 58-62 第47卷, * |
| 朱学芝等: "微生态制剂对凡纳滨对虾生长及水质的影响", 《中山大学学报(自然科学版)》 * |
| 胡毅: "凡纳滨对虾饲料配方优化及几种饲料添加剂的应用", 《中国博士学位论文全文数据库 农业科技辑》 * |
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Application publication date: 20121121 |