CN102731615B - Prrsv的检测试剂和检测方法 - Google Patents
Prrsv的检测试剂和检测方法 Download PDFInfo
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
试剂盒组份 | 规格 | 数量 |
抗原包被板 | 96孔/块 | 5块 |
样品稀释液 | 120ml/瓶 | 1瓶 |
洗液 | 120ml/瓶 | 1瓶 |
标准阳性血清 | 1ml/瓶 | 1瓶 |
标准阴性血清 | 1ml/瓶 | 1瓶 |
酶标抗体结合物 | 70ml/瓶 | 1瓶 |
显色液 | 70ml/瓶 | 1瓶 |
终止液 | 40ml/瓶 | 1瓶 |
使用说明书 | 份 | 1 |
Claims (32)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110143363.7A CN102731615B (zh) | 2011-05-27 | 2011-05-27 | Prrsv的检测试剂和检测方法 |
TW101118904A TW201300421A (zh) | 2011-05-27 | 2012-05-25 | 用於偵測豬繁殖及呼吸道綜合症病毒(prrsv)之試劑及方法 |
PCT/CN2012/076181 WO2012163263A1 (en) | 2011-05-27 | 2012-05-28 | Reagents and methods for prrsv detection |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110143363.7A CN102731615B (zh) | 2011-05-27 | 2011-05-27 | Prrsv的检测试剂和检测方法 |
Publications (2)
Publication Number | Publication Date |
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CN102731615A CN102731615A (zh) | 2012-10-17 |
CN102731615B true CN102731615B (zh) | 2015-05-06 |
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CN201110143363.7A Active CN102731615B (zh) | 2011-05-27 | 2011-05-27 | Prrsv的检测试剂和检测方法 |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN102731615B (zh) |
TW (1) | TW201300421A (zh) |
WO (1) | WO2012163263A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103087996B (zh) * | 2013-01-18 | 2014-07-16 | 中国农业大学 | 重组猪繁殖与呼吸综合征病毒及其制备方法与应用 |
CN103725793B (zh) * | 2013-09-17 | 2016-08-17 | 广西壮族自治区动物疫病预防控制中心 | Prrsv的多重荧光定量rt-pcr扩增试剂 |
CN107782721A (zh) * | 2016-08-26 | 2018-03-09 | 韦彦余 | 一种基于膜的生物样品化学发光检测方法和相关检测试剂盒 |
CN109425738B (zh) * | 2017-09-01 | 2022-01-28 | 洛阳普泰生物技术有限公司 | 猪圆环病毒3型抗体检测试剂盒及其应用 |
CN109913587A (zh) * | 2019-03-25 | 2019-06-21 | 新乡学院 | 一种利用pcr-elisa检测prrsv的方法 |
CN111057145B (zh) * | 2019-11-22 | 2021-10-08 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 猪繁殖与呼吸综合征病毒Nsp2蛋白纳米抗体及其应用 |
CN110878379A (zh) * | 2019-12-11 | 2020-03-13 | 天津农学院 | 一种类nadc30 prrsv减毒活疫苗配套鉴别检测方法及其应用 |
CN111996213A (zh) * | 2020-02-06 | 2020-11-27 | 广西大学 | 猪繁殖与呼吸综合征病毒双荧光标记基因重组毒株的构建方法 |
CN114410843A (zh) * | 2022-01-29 | 2022-04-29 | 龙岩学院 | 同时鉴别四类毒株的荧光定量检测引物和探针组 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101045917A (zh) * | 2007-03-14 | 2007-10-03 | 中国动物疫病预防控制中心 | 猪繁殖与呼吸综合征疫苗、制备方法及应用 |
CN101633909A (zh) * | 2009-08-13 | 2010-01-27 | 武华 | 预防猪感染猪繁殖与呼吸综合征的减毒活疫苗 |
CN101988058A (zh) * | 2010-06-02 | 2011-03-23 | 黄鹏林 | 一种基因表达组合物、猪生殖与呼吸道综合症口服疫苗及其制备方法 |
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US7618797B2 (en) * | 1998-12-22 | 2009-11-17 | Pfizer Inc | Infectious cDNA clone of North American porcine reproductive and respiratory syndrome (PRRS) virus and uses thereof |
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2011
- 2011-05-27 CN CN201110143363.7A patent/CN102731615B/zh active Active
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2012
- 2012-05-25 TW TW101118904A patent/TW201300421A/zh unknown
- 2012-05-28 WO PCT/CN2012/076181 patent/WO2012163263A1/en active Application Filing
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