CN102687801A - Application of oudemansiella radicata mycelium polysaccharides to livestock feed - Google Patents

Application of oudemansiella radicata mycelium polysaccharides to livestock feed Download PDF

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CN102687801A
CN102687801A CN 201210150717 CN201210150717A CN102687801A CN 102687801 A CN102687801 A CN 102687801A CN 201210150717 CN201210150717 CN 201210150717 CN 201210150717 A CN201210150717 A CN 201210150717A CN 102687801 A CN102687801 A CN 102687801A
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polypeptide
polysaccharide
livestock
feed
livestock feed
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CN102687801B (en )
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侯潇
刘剑利
孟雪莲
徐成斌
曹向宇
赵中洲
郑勇
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辽宁大学
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Abstract

The invention relates to application of oudemansiella radicata mycelium polysaccharides to livestock feed. Weight ratio of additive to feed of the livestock feed containing the oudemansiella radicata mycelium polysaccharides is 1-50:100000. The oudemansiella radicata mycelium polysaccharides additive can be added to the feed for feeding livestock, and accordingly appetite whetting and growth promotion are achieved, immunity and body disease resistance of livestock are improved, meat of livestock is improved, and quality and quantity of poultry eggs can be increased. By scientific combination of the formula, growth promotion, immunity enhancement and survival rate increase can be realized for livestock without adding antibiotics and the like into the feed as additives, and drug residue in livestock products is avoided.

Description

长根菇菌丝体多糖在禽畜饲料中的应用 Long root application Mycelia polysaccharide livestock feed

技术领域 FIELD

[0001] 本发明属于饲料生产技术领域,具体地涉及以长根菇菌丝体多糖单用或与其它辅料混用作为禽畜饲料添加剤,在禽畜养殖中,添加到饲料中,达到增强禽畜免疫力,促进禽畜生长的目的。 [0001] The present invention belongs to the technical field of production of feed, in particular relates to a long root Mycelia polysaccharide alone or mixed with other excipients is added as a livestock feed Ji, in livestock breeding, added to the feed, poultry achieve enhanced livestock immunity, the purpose of promoting the growth of livestock.

背景技术 Background technique

[0002] 多糖(polysaccharide)是由多个单糖分子缩合、失水而成,是ー类分子机构复杂且庞大的糖类物质,广泛分布于动物、植物及微生物中。 [0002] The polysaccharide (polysaccharide) condensation of a plurality of monosaccharide molecules, obtained by dehydration, is ー molecule complex and bulky mechanism carbohydrate, widely distributed in animals, plants and microorganisms. 真菌多糖一般是指各种真菌的子实体和菌丝体所产生的ー类代谢产物。 Polysaccharides generally refers ー metabolic products of various fungi mycelia and fruiting bodies are produced. 目前,在全球范围内约有数千种真菌。 Currently, several thousand species of fungi on a global scale. 其中不仅有许多有实用价值的美味真菌,也有许多具有保健功能的真菌。 Not only are many delicious fungi have practical value, there are many fungi with health care function. 真菌多糖在治疗胃癌、结肠癌、肺癌等方面具有良好疗效。 Polysaccharides having a good effect in the treatment of gastric cancer, colon cancer, lung cancer. 作为免疫辅助药物,真菌多糖主要用来抑制肿瘤的发生、发展与转移,提高肿瘤对化疗药物的敏感性,改善患者的身体状况,延长其寿命。 As an immune adjuvant drug, fungal polysaccharides mainly used to suppress the occurrence, development and metastasis, improve the sensitivity of tumors to chemotherapeutic drugs, improve the patient's physical condition, prolong its life.

[0003] 长期以来,由于动物饲料中广泛使用抗生素、化学合成药物和激素等促生长保健齐U,导致畜禽和水产产品中药物残留严重,直接危害人体健康;畜禽产品品质下降,缺乏市场竞争力和信誉;细菌耐药性增强、耐药菌株增多,威胁人类安全;破坏生态环境。 [0003] For a long time, due to the widespread use of antibiotics in animal feed, chemical synthetic drugs and hormones and other growth-promoting health Qi U, leading to livestock and aquatic products, serious drug residues, directly endanger human health; decline in livestock product quality, lack of market competitiveness and credibility; enhanced bacterial resistance, resistant strains increased threat to human security; harm the environment.

[0004]目前,发达国家生产和应用的饲料添加剂有数千种,美国已批准使用的有260多种,每年消费饲料添加剂达650万吨;欧盟法定登记的饲料添加剂已有250种;日本有128种。 [0004] Currently, feed additives production and application of the developed countries there are thousands, the United States has approved the use of 260 kinds of annual consumption of 6.5 million tons of feed additives; EU legal registration of feed additives has 250 kinds; Japan 128 kinds. 我国已批准使用的饲料添加剂约80个品种,其中国内生产并已制定标准的仅40多种,其余是批准进ロ的国外产品。 China has approved the use of feed additives about 80 species, of which only 40 kinds of domestic production and have developed standards, and the rest is approved into the ro of foreign products. 与国外相比,我国的饲料添加剂品种少、产量小、价格高,且有许多产品尚未形成规模化生产,不能满足养殖业生产发展的需要。 Compared with foreign countries, China's feed additives varieties of small, low production, high prices, and there are many products not yet formed a large-scale production can not meet the needs of the development of aquaculture. 同时我国对饲料添加剂的基础研究不够,技术储备量低,许多新型的添加剂品种都不能生产,缺乏高水平的预混料配方,生产エ艺水平低,其整体水平与发达国家差距较大。 Meanwhile, China's basic research is not enough to feed additives, low technical reserves, many new varieties are not additive production, lack of a high level of premix formulations, Ester Arts low production level, which is a big gap between the overall level of the developed countries. 每年从国外进ロ添加剂耗费数亿元外汇。 Feed additives each year from abroad ro spend several hundred million dollars of foreign exchange.

[0005] 因此,开发具有抗氧化和增强免疫力等多种生物学功能的,代替化学合成药物,改善饲料风味,提高饲料的适ロ性,满足畜禽的营养需要,促进机体生长的天然绿色饲料添加剂具有更广阔的发展前景,也是亟待解决的问题。 [0005] Thus, the development of anti-oxidation and immune enhancement, and other biological functions, in place of synthetic drugs, to improve the flavor of feed, the feed to improve fitness ro, meet the nutritional needs of poultry, promote body growth natural green feed additives have a more broad prospects for development, it is also a serious problem.

发明内容 SUMMARY

[0006] 本发明的目的是提供一种绿色天然禽畜饲料添加剂,在禽畜养殖中,添加到禽畜饲料中,可增强禽畜免疫力,提高其抗病能力,促进禽畜生长。 [0006] The object of the present invention is to provide a natural green livestock feed additive in livestock breeding added to livestock feed to livestock can enhance the immune system, enhance their resistance to diseases, promote the growth of livestock.

[0007] 本发明采用的技术方案是:长根菇菌丝体多糖单用或与辅料混合使用制备长根菇菌丝体多糖型禽畜饲料添加剂,在日常的家禽,畜牧的饲养中,添加到饲料中。 [0007] aspect of the present invention is that: a long root Mycelia polysaccharide alone or mixed with excipients Changgen prepared using mycelium polysaccharide type livestock feed additives, in the daily poultry, livestock rearing added to feed.

[0008] 所述的辅料可以包括小米多肽、麦麸多肽、黒米多肽、意米多肽、光合细菌、粪链球菌、乳酸菌、双歧杆菌和芽孢杆菌。 Accessories [0008] The polypeptide may comprise millet, wheat bran polypeptide, polypeptide black rice, Italian rice polypeptide, photosynthetic bacteria, Streptococcus faecalis, Lactobacillus, Bacillus and Bifidobacterium.

[0009] 当长根菇菌丝体多糖与上述辅料混合使用制备长根菇菌丝体多糖型禽畜饲料添加剂时,按重量百分比禽畜饲料添加剂组成如下:长根菇菌丝体多糖65%-75%,小米多肽1%_5%,麦麸多肽1%_5%,黑米多肽1%_5%, [0009] When the root length Mycelia polysaccharide mixed with the adjuvants used Mycelia polysaccharide type livestock feed additive prepared root length, livestock feed additive by weight percent the following composition: Mycelia Changgen 65% polysaccharide 75%, 1% [5% polypeptide millet, wheat bran polypeptide _5% 1%, 1% black rice polypeptide _5%

意米多肽1%_5%,光合细菌1%_3%,粪链球菌1%-3%,乳酸菌1%-3%, Italian rice polypeptide _5% 1%, 1% _3% photosynthetic bacteria, Streptococcus faecalis 1% -3%, lactic acid 1% to 3%,

双歧杆菌1%_3%,芽孢杆菌1%-3%。 _3% 1% Bifidobacterium, Bacillus 1% -3%.

[0010] 以长根菇菌丝体多糖单用或与辅料混合使用作为禽畜饲料添加剤,应用于禽畜饲料时,禽畜饲料添加剂与禽畜饲料的重量份配比为:禽畜饲料添加剂1-50份,禽畜饲料100000 份。 [0010] In Long roots Mycelia polysaccharide alone or mixed with excipients used as livestock feed additives Ji, when applied to livestock feed, livestock feed additives and livestock feed parts by weight ratio of: livestock feed 1-50 parts additives, livestock feed 100,000 parts.

[0011] 本发明涉及的长根菇菌丝体多糖是由长根菇菌丝体提取的真菌多糖,以酸性多糖为主。 [0011] Mycelia Changgen the present invention the polysaccharide is an acidic polysaccharide mainly by the fungus mycelium polysaccharides long root extraction. 可以采用市购的或采用超声波-微波-复合酶酶解分步法的エ艺组合提取的长根菇菌丝体多糖。 It may be employed or commercially available ultrasonic Microwave - - Long roots Mycelia polysaccharide by enzymatic hydrolysis of the Ester arts stepwise process the extracted combinations.

[0012] 采用超声波-微波-复合酶酶解分步法的エ艺组合提取长根菇菌丝体多糖的步骤如下: [0012] The ultrasound - microwave - partial enzymatic hydrolysis step combinations Ester arts Mycelial extracts Changgen polysaccharide following steps:

1) 超声浸提:将长根菇菌丝体干粉溶于水中,料液比按质量比1:10-1:30,控制温度为50-90°C,pH 值4. 0-8. 0,超声波功率为100W-500W,超声时间为20min_60min ; 1) ultrasonic extraction: Mycelial long root powder was dissolved in water, a mass ratio of solid to liquid ratio of 1: 10-1: 30, controlled temperature 50-90 ° C, pH value of 4. 0-80. , ultrasonic power is 100W-500W, ultrasonic time 20min_60min;

2)微波辅助提取:微波功率300W-500W,微波时间为20min-60min ; 2) Microwave-assisted extraction: microwave power 300W-500W, microwave time is 20min-60min;

3)复合酶酶解提取:复合酶加入量为I. 0%-5. 0%,控制温度为30-60°C,pH 4. 0-8. 0,酶解时间为30min-60min ;抽滤,滤液旋转蒸发浓缩,加入4倍体积的95%こ醇沉淀多糖,2000rmp离心后去除上清液,冷冻干燥。 3) enzymatic hydrolysis extract: enzyme is added in an amount of I. 0% -5 0%, the temperature is controlled 30-60 ° C, pH 4. 0-8 0, enzymolysis time is 30min-60min; pumping. filtered off, the filtrate was concentrated by rotary evaporation, the alcohol was added 95% ko 4 volumes of polysaccharide precipitation, the supernatant was removed after centrifugation 2000rmp lyophilized. 所述的复合酶是纤维素酶与木瓜蛋白酶按质量比1:0. 5^2的混合。 The cellulase enzyme complex with papain mass ratio of 1: 05 ^ 2 of mixing.

[0013] 本发明采用的辅料小米多肽,麦麸多肽,黒米多肽和薏米多肽,可以采用市购的或采用提取蛋白质后酶解法制备的生物活性肽。 [0013] The materials employed in the present invention polypeptide millet, wheat bran polypeptide, black rice and barley polypeptide polypeptide, or may be employed using commercially available protein extraction after hydrolysis by biologically active peptides.

[0014] 本发明具开创性的技术是将长根菇菌丝体多糖作为禽畜饲料添加剂的核心功能性原料单用或与其它营养素结合,根据现代动物营养学模型合理搭配、科学组合应用,形成具有提高禽畜机体功能的多糖型混合饲料添加剤。 Technology [0014] The present invention is a groundbreaking core functional material Changgen Mycelia polysaccharide as livestock feed additive alone or in combination with other nutrients, according to the modern science of animal nutritional with a reasonable combination of scientific applications, forming a polysaccharide-type mixing livestock feed to improve the body function Ji added.

[0015] 本发明的含有长根菇菌丝体多糖的禽畜饲料添加剂,在现有家禽、牲畜的饲养中,可以与现有的任意的禽畜饲料混合,都可以达到增强禽畜免疫力,提高抗病能力,促进禽畜生长的目的。 [0015] livestock feed additives comprising Long roots mycelium polysaccharide of the present invention, in conventional breeding poultry, livestock may be mixed with any conventional livestock feed, livestock can achieve enhanced immunity improve disease resistance, the purpose of promoting the growth of livestock.

[0016] 添加本发明长根菇菌丝体多糖型饲料添加剂不影响此后对饲料其他方式的加工。 [0016] The present invention Changgen added Mycelia feed additive does not affect the polysaccharide Thereafter otherwise processed feed.

[0017] 本发明和现有技术相比有如下优点:添加长根菇菌丝体多糖型禽畜饲料添加剂的饲料喂养禽畜,具有增进食欲、促进生长、抗病健体功效,可以提高禽畜的免疫力,改善禽畜肉质,还可以提高禽类产蛋品质及数量。 [0017] and the present invention has the following advantages over the prior art: Mycelia added Changgen polysaccharide type feeding livestock feed livestock feed additives, have appetite, promote growth, antiviral efficacy fitness can be improved poultry animal's immune system, improve livestock meat, poultry egg production can also improve the quality and quantity. 通过配方的科学组合,从而不需要在饲料中添加抗生素等化学药物作为饲料添加剂也可达到对禽畜的促进生长和提高免疫力、成活率的功效,并避免了禽畜产品中的药物残留。 Scientific combination formula, eliminating the need to add chemicals and antibiotics used as feed additives to promote the growth of poultry can be achieved and improve the effectiveness of the immune system, the survival rate in the feed, and to avoid drug residues in livestock products. 长根菇菌丝体多糖来源简单,通过生物发酵法制备长根菇菌丝体,提取方法成熟,成本较低,添加到禽畜饲料中,减少常规饲料及抗生素的应用,提高禽畜食用安全性,降低饲料成本。 Long roots Mycelia simple polysaccharide sources, prepared by fermentation mycelium root length, mature extraction method, low cost, added to livestock feed, feed and reduced using the conventional antibiotics, improved food safety of livestock and reduce feed costs.

具体实施方式 detailed description

[0018] 为了便于理解本发明,特例举以下实施例。 [0018] In order to facilitate understanding of the present invention, the following examples mentioned Patent. 其作用被理解为是对本发明的阐释而非对本发明的任何形式的限制。 Its role is not to be construed as limiting the invention to illustrate the present invention in any form.

[0019] 实施例I将长根菇菌丝体多糖单用作为禽畜饲料添加剤,按重量比10:100000的比例与常规的猪饲料混合均匀,饲喂猪。 [0019] Example I mycelium long root polysaccharide alone as a livestock feed additive Ji, ratio by weight ratio of 10: 100,000 mixed with conventional homogeneous pig feed, feeding pigs.

[0020] 还可以将上述混合均匀的饲料再按常规エ艺制备仔猪饲料,直接饲喂仔猪。 [0020] The above may be mixed feed prepared in the usual Ester arts piglet feed, direct-fed piglets.

[0021] 本实施例采用以超声波-微波-复合酶酶解分步法的エ艺组合提取的长根菇菌丝体多糖,步骤如下: [0021] In the present embodiment uses ultrasound - microwave - partial enzymatic hydrolysis step the extracted combination Ester arts Changgen mycelium polysaccharide, the following steps:

1) 超声浸提:将长根菇菌丝体干粉充分溶于水中,料液比按质量比1:10-1:30,在该料液比条件下,进行热水浸提多糖,控制浸提温度为50-90°C,浸提pH值4. 0-8. 0,超声波功率为100W-500W之间,超声时间为20min-60min ; 1) ultrasonic extraction: Mycelial long root dry sufficiently soluble in water, a mass ratio of solid to liquid ratio of 1: 10-1: 30, in the solid-liquid ratio conditions, a polysaccharide extracted by hot water, dip control extraction temperature is 50-90 ° C, the pH of the leaching 4. 0-80, ultrasonic power between 100W-500W, ultrasonic time 20min-60min.;

2) 然后微波辅助提取:控制微波功率300W-500W之间,微波提取时间为20min_60min ; 2) Microwave-assisted extraction is then: controlling the microwave power between 300W-500W, microwave extraction time 20min_60min;

3) 最后复合酶酶解提取:复合酶(纤维素酶与木瓜蛋白酶质量比为1:1)加入量为 3) Finally, enzymatic hydrolysis extract: enzyme (cellulase papain mass ratio of 1: 1) was added in an amount of

I. 0%-5. 0% (w%),酶解温度为30-600C,酶解pH范围值4. 0-8. O酶解时间为30min_60min。 I. 0% -5. 0% (w%), hydrolysis temperature 30-600C, pH value range hydrolysis 4. 0-8. O hydrolysis time 30min_60min. 抽滤,滤渣反复提取2次,合并滤液。 Filtration, the filter residue repeatedly extracted twice, the combined filtrate. 滤液旋转蒸发浓缩至原体积的1/4后,加入4倍体积的95%こ醇沉淀多糖,2000rmp离心后去除上清液,冷冻干燥。 The filtrate was concentrated by rotary evaporation to 1/4 of its original volume, added to 4 volumes of 95% alcohol precipitation ko polysaccharide supernatant was removed after centrifugation 2000rmp lyophilized. 经过该エ艺组合提取的长根菇菌丝体多糖得率明显提高,收率达到85%以上。 After the combination of the extracted long Ester arts Mycelia root polysaccharide yield significantly improved yield of more than 85%.

[0022] 长根菇菌丝体多糖也可以采用市购的产品。 [0022] Mycelia root length polysaccharides may be used commercially available products.

[0023] 实施例2 [0023] Example 2

(一)长根菇菌丝体多糖型禽畜饲料添加剂O长根菇菌丝体多糖型禽畜饲料添加剂按重量百分比组成如下: (A) Long roots Mycelia polysaccharide type livestock feed additive Changgen O Mycelia polysaccharide type livestock feed additive the following composition in percentage by weight:

长根菇菌丝体多糖70%,小米多肽5%,麦麸多肽5%,黒米多肽5%, Long roots Mycelia 70% polysaccharide, 5% millet polypeptide, polypeptide 5% wheat bran, rice polypeptide black 5%,

意米多肽5%,光合细菌2%,粪链球菌2%,乳酸菌2%, Italian rice polypeptide 5%, 2% photosynthetic bacteria, Streptococcus faecalis 2%, 2% lactic acid,

双歧杆菌2%,芽孢杆菌2%。 Bifidobacterium 2%, 2% Bacillus.

[0024] 本实施例采用以超声波-微波-复合酶酶解分步法的エ艺组合提取的长根菇菌丝体多糖,制备方法同实施例I。 [0024] In the present embodiment uses ultrasound - microwave - Long roots mycelium polysaccharide, prepared by enzymatic hydrolysis of the sub-step extraction with a combination of arts Ester Example I.

[0025] 本实施例小米多肽,麦麸多肽,黒米多肽和薏米多肽采用提取蛋白质后酶解法制备的生物活性肽。 [0025] Example of the present embodiment polypeptide millet, wheat bran polypeptide, black rice and barley polypeptide polypeptides prepared using bioactive peptides extracted after hydrolysis by the protein. 以提取蛋白质后酶解法制备生物活性肽的步骤如下:将麦麸、小米、黒米、意米粉碎,按与NaOH溶液的重量比I : 10〜20,分别加入pH 11的NaOH溶液,提取温度为45-55°C,时间为2-3h,调pH至等电点,离心收集沉淀,冷冻干燥分别制得麦麸、小米、黒米、意米蛋白粉末;取麦麸、小米、黒米、意米蛋白粉末,分别加入碱性蛋白酶酶解,酶解条件为:PH 8,酶活添加量为6000u/g,时间2. 5-3. 5h,温度50 V ;然后加入复合风味蛋白酶酶解,酶解条件为:pH 6,酶活添加量为6000u/g,时间1-1. 5h,温度55 V ;将酶解后的酶解液加热到100°C,保持10分钟;采用截留分子量为IOkDa超滤板超滤,收集分子量为IOkDa以下的酶解液;冷冻干燥分别制得小米多肽,麦麸多肽,黒米多肽和薏米多肽。 In the step of preparation of bioactive peptides extracted after hydrolysis by the protein is as follows: wheat bran, millet, black rice, Italian rice pulverized NaOH solution by weight ratio of I: 10~20, respectively NaOH solution was added to pH 11, extraction temperature 45-55 ° C, 2-3h time, the pH was adjusted to the isoelectric point, the precipitate was collected by centrifugation, freeze-dried bran were prepared, millet, black rice, Italian rice protein powder; taking wheat bran, millet, black rice, Italian rice protein powders were added alkaline protease hydrolysis, hydrolysis conditions: PH 8, enzyme added amount 6000u / g, the time 2. 5-3 5h, a temperature of 50 V; flavourzyme enzyme complex was then added, enzymes. solution conditions: pH 6, enzyme added amount 6000u / g, time 1-1 5h, a temperature of 55 V; solution after the enzymatic digestion was heated to 100 ° C, held for 10 min; molecular weight cutoff using IOkDa. ultrafiltration ultrafiltration plate, collecting a molecular weight of less IOkDa enzymatic hydrolyzate; lyophilized polypeptide are prepared millet, wheat bran polypeptide, black rice and barley polypeptide polypeptide.

[0026] 小米多肽,麦麸多肽,黒米多肽和薏米多肽也可以采用市购的产品。 [0026] polypeptides millet, wheat bran peptide, polypeptide black rice and barley polypeptides may also be used commercially available products.

[0027] 2)制备方法:将长根菇菌丝体多糖与小米多肽,麦麸多肽,黒米多肽,意米多肽,光合细菌,粪链球菌,乳酸菌,双歧杆菌和芽孢杆菌按上述的配比取料,混合均匀即可。 Preparation of [0027] 2) Method: The root length Mycelia polysaccharide polypeptide millet, wheat bran polypeptide, polypeptide black rice, Italian rice polypeptide, photosynthetic bacteria, Streptococcus faecalis, Lactobacillus, Bifidobacterium, and Bacillus the above-described feature than reclaimers, evenly mixed.

[0028] (ニ)混合禽畜饲料 [0028] (Ni) mixing livestock feed

将长根菇菌丝体多糖型禽畜饲料添加剂按重量比30:100000的比例与肉食鸡全价饲料混合均匀,制备肉食鸡饲料,直接饲喂鸡。 Mycelia long root polysaccharide type livestock feed additive by weight ratio of 30: 100,000 chickens complete feed ratio uniformly mixed to prepare feed chickens, direct-fed chicken. [0029] 实施例3 [0029] Example 3

(一)长根菇菌丝体多糖型禽畜饲料添加剂O长根菇菌丝体多糖型禽畜饲料添加剂按重量百分比组成如下: (A) Long roots Mycelia polysaccharide type livestock feed additive Changgen O Mycelia polysaccharide type livestock feed additive the following composition in percentage by weight:

长根菇菌丝体多糖75%,小米多肽3%,麦麸多肽3%,黒米多肽2%, Long roots 75% mycelium polysaccharides, polypeptides millet 3%, 3% wheat bran polypeptide, polypeptide 2% black rice,

意米多肽2%,光合细菌3%,粪链球菌3%,乳酸菌3%, Italian rice polypeptide 2%, 3% photosynthetic bacteria, Streptococcus faecalis 3%, 3% lactic acid,

双歧杆菌3%,芽孢杆菌3%。 Bifidobacterium 3%, 3% Bacillus.

[0030] 本实施例采用以超声波-微波-复合酶酶解分步法的エ艺组合提取的长根菇菌丝体多糖,制备方法同实施例I。 [0030] In the present embodiment uses ultrasound - microwave - Long roots mycelium polysaccharide, prepared by enzymatic hydrolysis of the sub-step extraction with a combination of arts Ester Example I.

[0031] 本实施例采用以提取蛋白质后酶解法制备生物活性肽的方法制备的小米多肽,麦麸多肽,黒米多肽和薏米多肽,制备方法同实施例2。 [0031] The present embodiment employs the method of millet polypeptide produced proteins were extracted after hydrolysis by preparation of biologically active peptides, polypeptides wheat bran, black rice and barley polypeptide polypeptides prepared in Example 2.

[0032] 当然,长根菇菌丝体多糖、小米多肽、麦麸多肽、黒米多肽和薏米多肽也可以采用市购的产品。 [0032] Of course, the root length mycelium polysaccharides, polypeptides millet, wheat bran polypeptide, black rice and barley polypeptide polypeptide may be used commercially available products.

[0033] 2)制备方法:将长根菇菌丝体多糖与小米多肽,麦麸多肽,黒米多肽,意米多肽,光合细菌,粪链球菌,乳酸菌,双歧杆菌和芽孢杆菌按上述的配比取料,混合均匀即可。 Preparation of [0033] 2) Method: The root length Mycelia polysaccharide polypeptide millet, wheat bran polypeptide, polypeptide black rice, Italian rice polypeptide, photosynthetic bacteria, Streptococcus faecalis, Lactobacillus, Bifidobacterium, and Bacillus the above-described feature than reclaimers, evenly mixed.

[0034] (ニ)混合禽畜饲料 [0034] (Ni) mixing livestock feed

将长根菇菌丝体多糖型禽畜饲料添加剂按重量比50:100000的比例与蛋鸡全价饲料混合均匀,制备蛋鸡饲料,直接饲喂蛋鸡。 Mycelia long root polysaccharide type livestock feed additive by weight ratio of 50: 100,000 complete feed ratio laying uniformly mixed to prepare a layer feed, direct-fed hens.

[0035] 应用效果 [0035] Application results

选取7日肉鸡54只,按体重、性别均等的原则随机分为3组,每组18只肉鸡,设3个重复组,每组重复6只。 Select the 7th broiler 54 according to body weight, the principle of equality of sex were randomly divided into 3 groups of 18 broilers, 3 replicates groups, each repeated six. 对照组,饲喂正常肉鸡饲料,试验组分为两组,试验组I,将长根菇菌丝体多糖按10:100000的比例与动物日粮混合均匀,再按常规エ艺制备饲料,直接饲喂;试验组2,将长根菇菌丝体多糖型混合饲料添加剂按10:100000的比例与动物日粮混合均匀,再按常规エ艺制备饲料,直接饲喂。 Control group, were fed normal broiler feed, divided into two test groups, the test group I, the root length Mycelia polysaccharide ratio of 10: 100000 animal diets mixed, prepared in the usual feed Ester arts, direct feeding; test group 2, the root length mycelia polysaccharide type feed additive mixing ratio of 10: 100000 animal diets mixed, prepared in the usual feed Ester arts, direct-fed. 饲养期共21天,每天记录采食量。 Feeding period for 21 days, recording daily food intake. 在试验开始时(7d),14d、21d和28d分别空腹称重,并处死肉鸡,剥取胸腺、脾脏和法氏囊,并将周围组织剥离干净后分析天平称重,按下式计算免疫脏器指数,显微镜观察肉鸡内脏器官有无异常和病变。 At the start of the test (7d), 14d, 21d and 28d, respectively fasting weighed, sacrificed and broilers, stripping thymus, spleen and bursa, and the surrounding tissue after release analytical balance weighing a clean, dirty immunity following formula indices, microscopic observation of internal organs broiler whether the abnormal and diseased. 研究长根菇菌丝体多糖、长根菇菌丝体多糖型混合饲料添加剂对肉鸡生长及免疫功能的影响,试验结果见表I、表2。 Root of Long polysaccharide mycelium, mycelium affect root length polysaccharide type mixing feed additive on growth and immune function, the test results shown in Table I, Table 2.

Figure CN102687801AD00061

[0036] 试验组与对照组相比,长根菇菌丝体多糖对肉鸡体重明显增加,其中长根菇菌丝体多糖型混合饲料添加剂对肉鸡体重增加更加明显。 [0036] The test group compared with the control group, root length Mycelia significant weight gain in broiler polysaccharide, wherein the polysaccharide Changgen hybrid mycelium on broiler feed additives more apparent weight gain.

Figure CN102687801AD00071

[0037] 肉鸡食用了长根菇菌丝体多糖及混合型饲料添加剂后,脾脏指数、胸腺指数和法氏囊指数都有所増加,说明长根菇菌丝体多糖及混合型饲料添加剂可显著提高肉鸡免疫器官指数,其中长根菇菌丝体多糖混合型饲料添加剂对提高肉鸡免疫器官指数效果更为显著。 After [0037] consumption of broiler Changgen Mycelia hybrid polysaccharides and feed additives, the index of spleen, thymus and bursa to increase in both the described Changgen Mycelia polysaccharides and mixed feed additives can be significantly increase in immune organ index, root length wherein mycelia polysaccharide mixed feed additive to increase in immune organ index effect is more significant.

Claims (6)

  1. 1.长根菇菌丝体多糖在禽畜饲料中的应用,其特征在于:长根菇菌丝体多糖单用或与辅料混合使用制备长根菇菌丝体多糖型禽畜饲料添加剤。 1. Application of mycelium polysaccharide livestock feed root length, wherein: the root length Mycelia polysaccharide alone or mixed with excipients added Ji root length was prepared using mycelium polysaccharide type livestock feed.
  2. 2.按照权利要求I所述的应用,其特征在于:所述的辅料包括小米多肽、麦麸多肽、黑米多肽、意米多肽、光合细菌、粪链球菌、乳酸菌、双歧杆菌和芽孢杆菌。 2. The use as claimed in claim I, wherein: said polypeptide excipients include millet, wheat bran polypeptide, polypeptide black rice, Italian rice polypeptide, photosynthetic bacteria, Streptococcus faecalis, Lactobacillus, Bifidobacterium, and Bacillus .
  3. 3.按照权利要求2所述的应用,其特征在于:长根菇菌丝体多糖与辅料混合使用制备的长根菇菌丝体多糖型禽畜饲料添加剤,按重量百分比组成如下: 长根菇菌丝体多糖65%-75%,小米多肽1%_5%,麦麸多肽1%_5%,黑米多肽1%_5%, 意米多肽1%_5%,光合细菌1%_3%,粪链球菌1%_3%,乳酸菌1%-3%, 双歧杆菌1%_3%,芽孢杆菌1%-3%。 3. The use according to claim 2, wherein: the root length Mycelia polysaccharide excipient mixture prepared root length polysaccharide type Mycelia added Ji livestock feed, the following composition by weight percent: Changgen mycelia 65-75% polysaccharide, polypeptide 1% [5% millet, wheat bran polypeptide _5% 1%, 1% black rice polypeptide _5%, Italian rice polypeptide _5% 1%, 1% _3% photosynthetic bacteria, fecal Streptococcus _3% 1%, lactic acid 1% to 3%, 1% _3% Bifidobacterium, Bacillus 1% -3%.
  4. 4.按照权利要求1、2或3所述的应用,其特征在于:长根菇菌丝体多糖型禽畜饲料添加剂与禽畜饲料的重量份配比为:禽畜饲料添加剂1-50份,禽畜饲料100000份。 4. Use according to claim 2 or claim 3, wherein: the root parts by weight of a long polysaccharide type Mycelia livestock feed additives and livestock feed ratio: 50 parts livestock feed additives , 100,000 parts of livestock feed.
  5. 5.按照权利要求1、2或3所述的应用,其特征在于:所述的长根菇菌丝体多糖的制备方法如下: 1)超声浸提:将长根菇菌丝体干粉溶于水中,料液比按质量比1:10-1:30,控制温度为50-90°C,pH 值4. 0-8. 0,超声波功率为100W-500W,超声时间为20min_60min ; 2)微波辅助提取:微波功率300W-500W,微波时间为20min-60min ; 3)复合酶酶解提取:复合酶加入量为I. 0%-5· 0%,控制温度为30-60°C, pH 4. 0-8. 0,酶解时间为30min-60min ;抽滤,滤液旋转蒸发浓缩,加入4倍体积的95%こ醇沉淀多糖,2000rmp离心后去除上清液,冷冻干燥。 5. Use according to claim 2 or claim 3, wherein: the method of preparing a long root Mycelial polysaccharides as follows: 1) ultrasound extraction: The root length was dissolved in dry mycelium the water liquid ratio mass ratio of 1: 10-1: 30, controlled temperature 50-90 ° C, pH value of 4. 0-80, ultrasonic power is 100W-500W, ultrasonic time 20min_60min; 2) microwave. assisted extraction: microwave power 300W-500W, microwave time is 20min-60min; 3) enzymatic hydrolysis extract: enzyme is added in an amount of I. 0% -5 · 0%, the temperature is controlled 30-60 ° C, pH 4 . 0-80, enzymolysis time is 30min-60min; filtration, the filtrate was concentrated by rotary evaporation, the alcohol was added 95% ko 4 volumes of polysaccharide precipitation, the supernatant was removed after centrifugation 2000rmp lyophilized.
  6. 6.按照权利要求5所述的应用,其特征在于:所述的复合酶是纤维素酶与木瓜蛋白酶按质量比1:0. 5〜2的混合。 6. The use according to claim 5, characterized in that: said composite is a cellulase enzyme papain mass ratio of 1: 0 mixture of 5~2.
CN 201210150717 2012-05-16 2012-05-16 Application of oudemansiella radicata mycelium polysaccharides to livestock feed CN102687801B (en)

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CN1793380A (en) * 2004-12-26 2006-06-28 陆晓民 Process and application for producing fermant protein peptide
WO2006119774A1 (en) * 2005-05-13 2006-11-16 Medimush As Feed or food products comprising fungal material
CN1920049A (en) * 2005-08-26 2007-02-28 上海弭阳生物技术有限公司 Small molecule bioactive peptide, preparation method, composition and application thereof
WO2011032244A1 (en) * 2009-09-17 2011-03-24 Blazei Brazil Ltda Flours produced from fungus myceliated grain
CN102268467A (en) * 2010-06-02 2011-12-07 湖南民康生物技术研究所 Mycelial extract and its application Polysaccharide

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1793380A (en) * 2004-12-26 2006-06-28 陆晓民 Process and application for producing fermant protein peptide
WO2006119774A1 (en) * 2005-05-13 2006-11-16 Medimush As Feed or food products comprising fungal material
CN1920049A (en) * 2005-08-26 2007-02-28 上海弭阳生物技术有限公司 Small molecule bioactive peptide, preparation method, composition and application thereof
WO2011032244A1 (en) * 2009-09-17 2011-03-24 Blazei Brazil Ltda Flours produced from fungus myceliated grain
CN102268467A (en) * 2010-06-02 2011-12-07 湖南民康生物技术研究所 Mycelial extract and its application Polysaccharide

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