CN102668986A - Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva - Google Patents
Direct rooting method for tissue culture cluster seedlings of hemerocallis fulva Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 240000009206 Hemerocallis fulva Species 0.000 title 1
- 235000002941 Hemerocallis fulva Nutrition 0.000 title 1
- 241000756137 Hemerocallis Species 0.000 claims abstract description 21
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 13
- 239000002609 medium Substances 0.000 claims abstract description 9
- 239000002985 plastic film Substances 0.000 claims abstract description 8
- 229920006255 plastic film Polymers 0.000 claims abstract description 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 6
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 6
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 6
- 239000010455 vermiculite Substances 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 5
- 238000005507 spraying Methods 0.000 claims abstract description 5
- 238000004080 punching Methods 0.000 claims abstract description 4
- 239000000758 substrate Substances 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000006870 ms-medium Substances 0.000 claims description 5
- 230000003050 macronutrient Effects 0.000 claims description 3
- 235000021073 macronutrients Nutrition 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000004069 differentiation Effects 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 230000007704 transition Effects 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 241000234280 Liliaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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Abstract
一种大花萱草组培丛生苗直接生根的方法，按照如下步骤进行：将大花萱草外植体在培养基上诱导产生愈伤组织，在分化培养基上培养丛生芽；将愈伤组织切块，并转接到培养基上壮苗培养；将该丛苗进行过渡移栽；过渡移栽时，将生根的丛生苗载入以蛭石为基质的栽培槽中，用地膜覆盖栽培槽；在以后的10天内，通过薄膜打孔的方法逐步降低栽培槽内湿度至温室正常湿度，将地膜揭去，同时在叶面开始喷施营养液。利用本发明所公开的这种大花萱草组培丛生苗直接生根的方法培养丛生苗，不仅繁殖倍数高，还不受季节限制，加快其繁殖速度，可满足园林绿化的需求；本发明简化了组培操作程序，降低了成本，提高了组培苗的移栽效率和成活率。A method for direct rooting of Hemerocallis grandis tissue-cultured clustered seedlings, which is carried out according to the following steps: inducing callus from explants of Hemerocallis grandis on a culture medium, and cultivating clustered shoots on a differentiation medium; cutting the callus block, and transferred to the culture medium for strong seedling cultivation; the cluster seedlings were transitionally transplanted; during transitional transplantation, the rooted cluster seedlings were loaded into a cultivation tank with vermiculite as the substrate, and the cultivation tank was covered with plastic film; In the next 10 days, gradually reduce the humidity in the cultivation tank to the normal humidity in the greenhouse by punching holes in the film, remove the plastic film, and start spraying the nutrient solution on the leaves at the same time. Utilize the method for directly rooting the hemerocallis grandiflorum tissue culture clustered seedlings disclosed in the present invention to cultivate the clustered seedlings, not only the multiplication rate is high, but also not limited by seasons, the speed of reproduction can be accelerated, and the needs of landscaping can be met; the present invention simplifies The tissue culture operation procedure reduces the cost and improves the transplanting efficiency and survival rate of the tissue culture seedlings.
Description
技术领域 technical field
本发明涉及一种观赏植物组织培养丛生苗的生根方法，特别是大花萱草组培丛生苗直接生根的方法，适于宿根花卉大花萱草的各品种。 The invention relates to a rooting method for tissue-cultured clustered seedlings of ornamental plants, in particular to a method for directly rooting the tissue-cultured clustered seedlings of Hemerocallis grandis, which is suitable for various varieties of Hemerocallis grandis.
背景技术 Background technique
大花萱草(Hemerocallis hybrida hort.)系百合科萱草属多年生草本宿根花卉，具有较强的抗旱、抗寒、抗盐碱、抗病虫害等特性。既可大面积种植在草坪周际，也可群植于草坪中央，增加立体效果，是园林绿化不可多得的宿根花卉新品种。经栽培观察，一些大花品种结实率低，只能采用分株繁殖，而且年增殖率仅2-3倍，繁殖率低，远远不能满足市场的大量需求。 Hemerocallis hybrida hort. is a perennial herbaceous perennial flower of the Liliaceae Hemerocallis genus, which has strong drought resistance, cold resistance, salt resistance, and pest resistance. It can be planted in a large area around the lawn or in the center of the lawn in groups to increase the three-dimensional effect. It is a rare new variety of perennial flowers in landscaping. Through cultivation observation, some large-flowered varieties have low seed setting rate, and can only be propagated by division, and the annual multiplication rate is only 2-3 times, and the reproduction rate is low, which is far from meeting the large demand of the market. the
发明内容 Contents of the invention
本发明是针对目前大花萱草分株繁殖的缺陷，提供一种繁殖率高、繁殖周期短、成活率高的大花萱草组培丛生苗直接生根的方法。 The invention aims at the defects of current Hemerocallis grandiflorum ramet propagation, and provides a method for direct rooting of Hemerocallis grandiflorum tissue-cultured cluster seedlings with high reproduction rate, short propagation period and high survival rate.
实现上述发明目的采用的技术方案是：一种大花萱草组培丛生苗直接生根的方法，按照如下步骤进行： The technical scheme that realizes above-mentioned invention object adopts is: a kind of method for the direct rooting of Hemerocallis grandiflorum tissue culture cluster seedling, carry out according to the following steps:
首先，大花萱草组培丛生苗直接生根： First, the tissue-cultured cluster seedlings of Hemerocallis grandiflorum take root directly:
①将大花萱草外植体在培养基MS+6-BA4.0mg·L-1+NAA0.4 mg·L-1上诱导产生愈伤组织，该愈伤组织经增殖培养后，在分化培养基MS+6-BA2.0 mg·L-1+NAA0.2 mg·L-1上培养20天后产生丛生芽，其培养环境为：温度23-25℃，光强2500lux，光周期12h/d； ①The explants of Hemerocallis grandiflora were induced to produce callus on the medium MS+6-BA4.0 mg·L -1 +NAA0.4 mg·L -1 , and the callus was proliferated and then differentiated After 20 days of culture on the base MS+6-BA 2.0 mg·L -1 +NAA 0.2 mg·L -1 , clustered shoots will be produced. The culture environment is: temperature 23-25 ℃, light intensity 2500lux, photoperiod 12h/d;
②将带有丛生芽的愈伤组织切块，并将切块转接到不含植物生产调节剂的MS培养基上壮苗培养，培养20天后形成整齐健壮的丛生苗，此培养过程中丛生苗开始生根； ②Cut the callus with clustered buds into pieces, and transfer the cuts to MS medium without plant production regulator for strong seedling cultivation, and form neat and strong clustered seedlings after 20 days of cultivation. During this culture process, clustered Seedlings begin to take root;
其次，丛生苗生根后移栽管理： Secondly, after the clustered seedlings take root, they are transplanted and managed:
①当每丛苗中只要有一株生根且根长达1cm时，将该丛苗进行过渡移栽；过渡移栽时，将生根的丛生苗连带培养基从培养瓶中取出，洗去根上的培养基，载入以蛭石为基质的栽培槽中，用地膜覆盖栽培槽，槽内湿度保持在90%以上； ①When there is only one root in each cluster of seedlings and the root length is 1cm, the cluster of seedlings is transitionally transplanted; when transitional transplanting, the rooted cluster of seedlings and the medium are taken out of the culture bottle, and the culture medium on the roots is washed away. base, loaded in the cultivation tank with vermiculite as the substrate, covered the cultivation tank with plastic film, and kept the humidity in the tank above 90%;
②在以后的10天内，通过薄膜打孔的方法逐步降低栽培槽内湿度至温室正常湿度，将地膜揭去，同时在叶面开始喷施1/2MS大量元素营养液，每周一次，其基质温度为10-25℃，30天后小苗进入常规管理。 ②In the next 10 days, gradually reduce the humidity in the cultivation tank to the normal humidity of the greenhouse by punching holes in the film, remove the plastic film, and start spraying 1/2MS macronutrient nutrient solution on the leaf surface once a week. The temperature is 10-25°C, and the seedlings enter the routine management after 30 days.
与现有的分株繁殖相比，利用本发明所公开的这种大花萱草组培丛生苗直接生根的方法培养丛生苗，不仅繁殖倍数高，还不受季节限制，加快其繁殖速度，可满足园林绿化的需求；本发明简化了组培操作程序，降低了成本，提高了组培苗的移栽效率和成活率。 Compared with the existing division propagation, the method of direct rooting of this Hemerocallis grandiflorum tissue culture cluster seedlings disclosed in the present invention is used to cultivate the cluster seedlings, not only the multiplication rate is high, but also not subject to seasonal restrictions, to speed up its propagation speed, which can Meet the needs of landscaping; the invention simplifies the tissue culture operation procedure, reduces the cost, and improves the transplanting efficiency and survival rate of the tissue culture seedlings.
具体实施方式 Detailed ways
下面结合实施例对本发明做进一步说明。 The present invention will be further described below in conjunction with embodiment.
这种大花萱草组培丛生苗直接生根的方法，按照如下步骤进行。 The method for the direct rooting of this Hemerocallis grandiflorum tissue culture cluster seedling is carried out according to the following steps.
首先，大花萱草组培丛生苗直接生根： First, the tissue-cultured cluster seedlings of Hemerocallis grandiflorum take root directly:
①将大花萱草外植体在培养基MS+6-BA4.0mg·L-1+NAA0.4 mg·L-1上诱导产生愈伤组织，该愈伤组织经增殖培养后，在分化培养基MS+6-BA2.0 mg·L-1+NAA0.2 mg·L-1上培养20天后产生丛生芽，其培养环境为：温度23-25℃，光强2500lux，光周期12h/d，即每天光照时间为12小时； ①The explants of Hemerocallis grandiflora were induced to produce callus on the medium MS+6-BA4.0 mg·L -1 +NAA0.4 mg·L -1 , and the callus was proliferated and then differentiated After 20 days of culture on the base MS+6-BA 2.0 mg·L -1 +NAA 0.2 mg·L -1 , clustered shoots will be produced. The culture environment is: temperature 23-25 ℃, light intensity 2500lux, photoperiod 12h/d, that is The daily light time is 12 hours;
②将带有丛生芽的愈伤组织切块，块体的大小为1cm3左右，并将切块转接到不含植物生产调节剂的MS培养基上壮苗培养，培养20天后形成整齐健壮的丛生苗，此培养过程中丛生苗开始生根； ②Cut the callus with clustered buds into pieces, the size of the block is about 1cm 3 , and transfer the cut pieces to the MS medium without plant production regulator for strong seedling cultivation, and form a neat and strong seedling after 20 days of cultivation The clustered seedlings, the clustered seedlings begin to take root during this cultivation process;
这种方法培养的丛生苗具有一条以上根的丛生苗可达98%以上，生根的丛生苗并不是每个单株都有根，丛生苗的各单株间不能分开。 The clustered seedlings that this method cultivates have the clustered seedlings of more than one root and can reach more than 98%, and the clustered seedlings that take root are not every individual plant all has root, can not separate between each single plant of clustered seedlings.
其次，丛生苗生根后移栽管理： Secondly, after the clustered seedlings take root, they are transplanted and managed:
①当每丛苗中只要有一株生根且根长达1cm时，将该丛苗进行过渡移栽；过渡移栽时，将生根的丛生苗连带培养基从培养瓶中取出，洗去根上的培养基，载入以蛭石为基质的栽培槽中，用地膜覆盖栽培槽，槽内湿度保持在90%以上； ①When there is only one root in each cluster of seedlings and the root length is 1cm, the cluster of seedlings is transitionally transplanted; when transitional transplanting, the rooted cluster of seedlings and the medium are taken out of the culture bottle, and the culture medium on the roots is washed away. base, loaded in the cultivation tank with vermiculite as the substrate, covered the cultivation tank with plastic film, and kept the humidity in the tank above 90%;
②在以后的10天内，通过薄膜打孔的方法逐步降低栽培槽内湿度至温室正常湿度，将地膜揭去，同时在叶面开始喷施1/2MS大量元素营养液，每周一次，其基质温度为10-25℃，30天后小苗进入常规管理。1/2MS大量元素营养液是利用组织培养上常用的MS培养基配方，即：MS基本培养基的大量元素、微量元素和铁盐成分作为喷施营养液，其中只有大量元素用量减半，微量元素和铁盐的用量不变； ②In the next 10 days, gradually reduce the humidity in the cultivation tank to the normal humidity of the greenhouse by punching holes in the film, remove the plastic film, and start spraying 1/2MS macronutrient nutrient solution on the leaf surface once a week. The temperature is 10-25°C, and the seedlings enter the routine management after 30 days. The 1/2MS macroelement nutrient solution is based on the MS medium formula commonly used in tissue culture, that is, the macroelements, trace elements and iron salt components of MS basic medium are used as the nutrient solution for spraying, in which only the amount of macroelements is halved, and the trace elements The amount of elements and iron salts remains unchanged;
经蛭石过渡的丛生苗，每个单株无论过渡前有没有根均生出新根，且很容易分开，每丛可产生3-8株独立的苗。 For the clustered seedlings transitioned by vermiculite, each individual plant will produce new roots no matter whether there are roots before the transition, and it is easy to separate, and each cluster can produce 3-8 independent seedlings.
实施例：大花萱草品种“红运”组培丛生苗的直接生根移栽。 Example: direct rooting and transplanting of clustered seedlings of Hemerocallis grandiflorum variety "Hongyun".
大花萱草“红运”幼嫩花柄为外植体诱导产生愈伤组织，经分化培养基产生丛生芽。将带有丛生芽的愈伤组织切块转接到不含植物生长调节剂的MS培养基上壮苗培养形成整齐而健壮的丛生苗，此过程中丛生苗开始生根。 The young flower stalks of Hemerocallis grandiflora "Hongyun" were explants to induce callus tissue, and clustered buds were produced by differentiation medium. The cut pieces of callus with clustered shoots were transferred to MS medium without plant growth regulators to form strong shoots to form neat and strong clustered shoots, and the clustered shoots began to root during this process.
丛生苗生根后，当根长至1cm时即可移栽。本年的2-4月份期间共移栽4次，过渡移栽4740丛生根苗，成活率达98％以上。经蛭石过渡20-30天后丛生苗的每个单株均生出新根，且很容易分开，每丛可产生3-8株独立的苗。 After the clustered seedlings take root, they can be transplanted when the roots grow to 1 cm. During the period from February to April this year, a total of 4 times were transplanted, and 4740 bushy root seedlings were transplanted in transition, with a survival rate of over 98%. After 20-30 days of vermiculite transition, each single plant of the clustered seedlings has new roots, and it is easy to separate, and each cluster can produce 3-8 independent seedlings. the
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| CN104542285A (en) * | 2014-12-30 | 2015-04-29 | 北京林业大学 | Method for tissue culture by applying leaves of hemerocallis middendorfii |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103270882B (en) * | 2013-06-07 | 2016-06-29 | 河北省林业科学研究院 | A kind of full-film covering method for culturing seedlings of hemerocailis middendorffi Time To Market in advance |
| CN103461135A (en) * | 2013-09-18 | 2013-12-25 | 宁波市农业科学研究院 | Method for propagating hemerocallis hybridus |
| CN103461135B (en) * | 2013-09-18 | 2015-10-07 | 宁波市农业科学研究院 | A kind of propagation method of hemerocailis middendorffi |
| CN103609444A (en) * | 2013-11-17 | 2014-03-05 | 浙江大学 | Tissue culture method for hemerocallis sempervirens araki |
| CN103609444B (en) * | 2013-11-17 | 2015-04-29 | 浙江大学 | Tissue culture method for hemerocallis sempervirens araki |
| CN104542285A (en) * | 2014-12-30 | 2015-04-29 | 北京林业大学 | Method for tissue culture by applying leaves of hemerocallis middendorfii |
| CN104542285B (en) * | 2014-12-30 | 2018-04-24 | 北京林业大学 | A kind of method of hemerocailis middendorffi leaf tissue culture |
| CN105340754A (en) * | 2015-12-04 | 2016-02-24 | 定州市绿谷农业科技发展有限公司 | Method suitable for industrialized propagation of hemerocallis middendorfii |
| CN106937595A (en) * | 2017-03-22 | 2017-07-11 | 江苏省农业科学院宿迁农科所 | A kind of purification and rejuvenation method of great Wu mouths day lily |
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