CN102507945A - Sulfamethazine enzyme-linked immunoassay kit - Google Patents
Sulfamethazine enzyme-linked immunoassay kit Download PDFInfo
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- CN102507945A CN102507945A CN2011103522531A CN201110352253A CN102507945A CN 102507945 A CN102507945 A CN 102507945A CN 2011103522531 A CN2011103522531 A CN 2011103522531A CN 201110352253 A CN201110352253 A CN 201110352253A CN 102507945 A CN102507945 A CN 102507945A
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Abstract
The invention relates to a sulfamethazine enzyme-linked immunoassay kit. A kit body comprises an elisa plate coated with sulfamethazine, a sulfamethazine specific antibody, enzyme conjugate, a sulfamethazine standard substance solution, a substrate A color-developing solution and a substrate B color-developing solution, a stop solution, concentrated washing liquid, concentrated complex liquid, and a concentrated extracting solution. The kit solves the problems of long operation time and complex pretreatment step in other kits, only 30 minutes is required by a whole operation process, and a special instrument is not required to be arranged on equipment during pretreatment; the kit can be operated easily and quickly and is suitable for field large-scale screening; and all reagents in the kit are working liquid, so the kit is easy to operate and is practicable.
Description
Technical field
The present invention relates to a kind of sulfadimidine enzyme-linked immunologic detecting kit.
Technical background
Sulfadimidine (S μ lfamethazine SM2), a kind of as sulfa drugs, its stable in properties, antimicrobial spectrum is wide, toxicity is little, oral easy absorption and cheap, be use the most extensively in the animal husbandry, one of medicine that application quantity is maximum.But because its action time and metabolism time in vivo is longer, easy in animal body residual, all possibly in human body, accumulate through any approach.Drug accumulation concentration surpasses certain value function of human body is harmful to, and accumulates for a long time then to cause the drug-fast generation of sulfa drug, causes the epidemic infection of drug-fast bacteria, and potential carcinogenicity is arranged.Therefore, European Union has formulated threshold limit value to the sulfa drugs in milk and the meat product, and promptly the sulfa drugs total amount must not surpass 100 μ g/ kg, and the concentration of single sulfa drugs must not surpass 25 μ g/ kg.In Dec, 2002, China Ministry of Agriculture announced files specify sulfamido MRL 100 μ g/kg in muscle, fat, liver and the kidney of all food animals No. 235, and sulfadimidine is listed as the object as key monitoring.
At present, the method for detection SM2 has microbial method, physical-chemical process; Like high pressure liquid chromatography (HPLC), gas-liquid chromatography (GLC), mass spectrum (MS), thin-layered chromatography (TLC) etc., the microbial method detection speed is very fast, but sulfa drug is lacked sensitivity and specificity; The physical chemistry rule exists pre-treatment loaded down with trivial details; Need characteristics such as expensive instrument and specialty operation thereof, detect comparatively difficulty of large quantities of samples, be unfavorable for applying on a large scale.
Summary of the invention
Technical matters to be solved by this invention provides a kind of sulfadimidine enzyme-linked immunologic detecting kit of easy and simple to handle, quick, suitable batch samples primary dcreening operation.
The present invention solves the problems of the technologies described above the technical scheme of taking:
A kind of sulfadimidine enzyme-linked immunologic detecting kit has a box body, comprises in the said box body:
(1) is coated with the ELISA Plate of sulfadimidine antigen;
(2) sulfadimidine specific antibody;
(3) enzyme labeling thing;
(4) sulfadimidine standard solution;
(5) A substrate colour developing liquid and B substrate colour developing liquid;
(6) stop buffer;
(7) concentrated cleaning solution;
(8) concentrate redissolution liquid;
(9) concentrated extracting solution.
Said sulfadimidine antigen is obtained through the coupling of diazotising method by sulfadimidine haptens and carrier protein, and said carrier protein is bovine serum albumin(BSA), human serum albumins, ovalbumin, rabbit anteserum albumen or mouse haemocyanin.
Said sulfadimidine specific antibody is for preparing monoclonal antibody or polyclonal antibody by said sulfadimidine antigen by the immunization method of routine; Its working fluid is the phosphate buffer that contains 0.1-5% (w/w) skimmed milk power of pH7.4, and the sulfadimidine specific antibody is 1:2000 (w/w) with the ratio of above-mentioned working fluid.
Said enzyme labeling thing is the anti-mouse of enzyme labeling or the antiantibody of anti-rabbit; Its working fluid is the phosphate buffer that contains the 0.1-5% skimmed milk power of pH7.4; The enzyme labeling thing is 1:4000 (w/w) with the ratio of above-mentioned working fluid, and marker enzyme is horseradish peroxidase or alkaline phosphatase.
Said sulfadimidine standard solution concentration is respectively 0 μ g/L, 0.5 μ g/L, 1.5 μ g/L, 4.5 μ g/L, 13.5 μ g/L, 40.5 μ g/L.
Said A substrate is the hydrogen peroxide of 0.06% (w/w), and the B substrate is 0.4% (w/w) tetramethyl benzidine.
Said concentrated extracting solution is 0.1M for NaOH concentration; The phosphate buffer of pH10; Said concentrated redissolution liquid is for containing the phosphate buffer of 0.1%-0.6% (w/w) DMSO, and said concentrated cleaning solution is the NaCl solution of 0.9% (w/w), and said stop buffer is the sulfuric acid solution of 2N.
The beneficial effect of sulfadimidine enzyme-linked immunologic detecting kit of the present invention is: what the residual enzyme linked immunological kit of detection sulfadimidine of the present invention adopted is direct competitive ELISA method; Overcome the problem of long, complex pretreatment of other kit running times, the whole operation process only needs 30min, and pre-treatment need not to be equipped with special instrument; Simple to operate, quick; Be fit to on-the-spot examination in enormous quantities, and each reagent of kit all is working fluid forms, operation is simple.
Description of drawings
Fig. 1 is the sulfadimidine canonical plotting.
Embodiment
A kind of sulfadimidine enzyme-linked immunologic detecting kit has a box body, comprises in the said box body:
(1) is coated with the ELISA Plate of sulfadimidine antigen;
(2) sulfadimidine specific antibody;
(3) enzyme labeling thing;
(4) sulfadimidine standard solution;
(5) A substrate colour developing liquid and B substrate colour developing liquid;
(6) stop buffer;
(7) concentrated cleaning solution;
(8) concentrate redissolution liquid;
(9) concentrated extracting solution.
One, the preparation process of kit of the present invention is following:
1, preparation is coated with the ELISA Plate of sulfadimidine coating antigen
Carbonate buffer solution with PH9.6 is diluted to 0.25mg/ml with the sulfadimidine coating antigen, and 100 μ l/ holes join on the ELISA Plate hole, and wherein the preferred ovalbumin of sulfadimidine coating antigen is as carrier protein; 4 ℃ spend the night or 37 ℃ hatch 2h, wash 4-5 time, clap to do; Add the confining liquid that contains 10% calf serum, 2h is hatched for 37 ℃ in 200 μ l/ holes; Room temperature is drained 5h in vacuum drying chamber, use the aluminium foil bag vacuum plastic sealing.
2, the preparation of antigen
(1) preparation of immunizing antigen
The SM2 that takes by weighing 55.6mg is dissolved among the HCl of 1 ml 1mol/L, as I liquid, and remains on 4 ℃; (2) take by weighing the 15mg sodium nitrite and be dissolved in the 1ml distilled water, slowly be added dropwise to A liquid, stir, keep the pH value to be lower than 3 while dripping; (3) reaction finishes the back and consumes excessive nitrous acid with sulfaminic acid, detect with starch potassium iodide paper, until ooze circle become from mazarine light yellow till, as B liquid; (4) take by weighing 50mgBSA and be dissolved in the 2ml 1mol/L pH9.6 sodium carbonate buffer, promptly be made into C liquid; (5) behind 4 ℃ of placement 1h, will dropwise add in the C liquid, keep pH9 in the course of reaction, at 4 ℃ of stirring reaction 6h through the B liquid that overactivation obtains; (6) PBS with 0.01mol/L dialysed 3 days, changed liquid every day 2 times, promptly was prepared into SM2-BSA.
(2) preparation of detection antigen
Preparation with immunizing antigen.
3, sulfadimidine MONOCLONAL ANTIBODIES SPECIFIC FOR
Animal immune: carrier protein is the immunogene of bovine serum albumin(BSA), the female Blab/c mouse in immune 6-8 age in week, and 2 week immunity are 1 time at interval, and three exempt from the back docking gets that hematometry is tired and inhibiting rate, and selection result is prepared to merge by best mouse.
Fusion of Cells: get the splenocyte of mouse and the SP2/O cell of this laboratory preservation; Merge; Indirect elisa method is measured supernatant and is chosen positive high hole; Through limiting dilution assay subclone is carried out in positive hole, until the hybridoma cell strain of setting up the monoclonal antibody that produces single anti-sulfadimidine.
A large amount of preparations of monoclonal antibody: choose individual bigger female Blab/c mouse, adopt in the body and induce the ascites method, prepare ascites in a large number, and through sad-ammonium sulfate precipitation purifying ascites, be divided into tubule ,-20 ℃ of preservations.
4, sample pre-treatments
(1) tissues such as meat, liver, kidney
Sample tissue except that homogenate behind the degrease, is taken by weighing the even pledge of 2g, add the extract of 4ml; Vortex vibration 5min; Room temperature (20-25 ℃) is the centrifugal 5min of 3000g down, gets supernatant 1ml and transfers PH at 7.0-7.5 with 1mol/L HCL, 70 ℃ of-80 ℃ of following water-bath 10min; Room temperature (20-25 ℃) is the centrifugal 5min of 3000g down, gets supernatant 50 μ l and detects.
(2) urine sample pre-treatment
The centrifugal 5min of urine sample 3000rpm to limpid, is got supernatant with redissolving after 5 times of the liquid dilutions, get 50 μ l and detect.
(3) feed pre-treatment
Sample tissue except that homogenate behind the degrease, is taken by weighing the even pledge of 2g, add the extract of 4ml; Vortex vibration 5min; Room temperature (20-25 ℃) is the centrifugal 5min of 3000g down, gets supernatant 1ml and transfers PH at 7.0-7.5 with 1mol/L HCL, 70 ℃ of-80 ℃ of following water-bath 10min; Room temperature (20-25 ℃) is the centrifugal 5min of 3000g down, gets supernatant 50 μ l and detects.
(4) milk pre-treatment
After 5 times of milk usefulness redissolution liquid dilutions, room temperature (20-25 ℃) is the centrifugal 5min of 3000g down, gets supernatant 50 μ l and detects.
5, the preparation of solution
(1) preparation of concentrated extracting solution
It is 0.1M that configuration contains NaOH concentration, the phosphate buffer of pH10.
(2) concentrate the preparation of redissolving liquid
Configuration contains the phosphate buffer of 0.1%-0.6% (w/w) DMSO, and PH is 7.4, and phosphate concn is 0.1M.
(3) preparation of sulfadimidine titer
Accurately take by weighing 100mg sulfadimidine standard sample, be dissolved in the 100ml methyl alcohol, be diluted to 40.5 μ g/L, 13.5 μ g/L, 4.5 μ g/L, 1.5 μ g/L, 0.5 μ g/L, 0 μ g/L with damping fluid.
6, the assembling of enzyme linked immunological kit
(1) each reagent all is sterile working in the bottling process.
(2) the lath vacuum plastic sealing in wall scroll 8 holes, 6 or 12 dresses; 2 bottles of 20ml of concentrated cleaning solution; 1 bottle of 20ml of concentrated extracting solution; Concentrate 1 bottle of 20ml of redissolution liquid; A, each one bottle of 7ml of B substrate colour developing liquid; 1 bottle of 3ml of sulfadimidine specific antibody working fluid; 1 bottle of 7ml of enzyme mark antiantibody working fluid; One bottle of 7ml of stop buffer; 6 bottles of sulfadimidine standard items, the 1ml/ bottle; 1 bottle of 100 μ g/L high concentration standard items, the 1ml/ bottle; Instructions is a; Microwell plate seals one of film, one of sheet frame.
(3) detect the encapsulation of qualified back, 4 ℃ of preservations.
7, kit test method
(1) reagent returns to room temperature in the taking-up kit, at least at indoor placement 30min;
(2) take out lath on demand, be placed on the ELISA Plate;
(3) on demand amount is pressed the 1:5 mixed with sulfadimidine specific antibody and enzyme mark thing;
(4) in the hole, add sulfadimidine standard items (or testing sample) 50 μ l successively, add potpourri (1:5) the 60 μ l of sulfadimidine specific antibody and enzyme mark thing, the mixing that vibrates gently, 37 ℃ of lucifuges are hatched 20min;
(5) washing is 4 times, claps and does;
(6) substrate A is developed the color liquid and substrate B colour developing liquid by the 1:1 mixed, 100 μ l/ holes add colour developing, and 37 ℃ of lucifuges are hatched 10min;
(7) add stop buffer 50 μ l cessation reactions, carry out dual wavelength under ELIASA 450nm and the 595nm and measure, carry out quantitative or qualitative according to typical curve.
(8) testing result analysis
Quantitative test: the mean light absorbency value of difference basis of calculation article and testing sample, the absorbance of standard items or sample (B) multiply by 100% again divided by the absorbance of 0 standard items, is the percentage absorbance, the percentage absorbance=(B/B0) * 100%.With the percentage absorbance is ordinate, and the logarithm of normal concentration is a horizontal ordinate, and the drawing standard curve is as shown in Figure 1.With the percentage absorbance substitution typical curve of sample to be tested, can obtain corresponding concentration, multiply by extension rate again and be the actual residual of sample.
Qualitative analysis: compare with the mean light absorbency value of sample to be tested and the absorbance of standard items, can draw the concentration range of sample to be tested.
Testing result also can be calculated with the computer software of specialty, and test specification is 0.5 μ g/ml-40.5 μ g/ml, and whole testing process only needs 30min.
Two, the sensitivity test of kit
Sensitivity is represented with LDL (mg/kg or μ g/kg).Measure blank samples such as 20 parts of chicken tissues, pork tissue, pork liver, pig urine, feed, milk respectively, and 20 parts of sulfadimidine 0 standard items.Ask the mean value (X) and the standard deviation (SD) of (B/B0) % value, obtain the corresponding standard items concentration of X-2SD value from typical curve, as the LDL of each sample.
50% inhibition concentration of this kit is in 1.3-2.0 μ g/L scope, and the detectability of tissue is about 1 μ g/L, urine 1.5 μ g/L, feed 3.0 μ g/L, milk 2.0 μ g/L.
Three, the degree of accuracy of kit test
From detect qualified kit, choose the same batch of ELISA Plate with different batches, measure 0.5 μ g/L, 4.5 μ g/L, three coefficient of variation of adding under the concentration of 13.5 μ g/L, each sample is done 20 multiple holes.In samples such as the pork tissue of being surveyed, pork liver, pig urine, feed, milk, the variation within batch coefficient all＜7.8%, interassay coefficient of variation is all＜9.1%.
Four, the recovery test of kit
The sample of measuring under 0.5 μ g/L, 4.5 μ g/L, three interpolations of the 13.5 μ g/L concentration adds recovery test, and each concentration is done 5 multiple holes, repeats 3 tests.The interpolation recovery of tissue such as meat, liver is 65%-110% as a result, and the interpolation recovery of pig urine, milk is 80%-120%, and the interpolation recovery in the feed is 55%-95%.
Five, the storage life of kit test
(1) kit is placed on 2 ℃-8 ℃ and preserved 6 months, during whenever detected once parameters such as the IC50% of mensuration kit, ODmax, the recovery at a distance from 1 month.
(2) kit is placed on 37 ℃ and placed 6 days, detect once every day, parameters such as the IC50% of mensuration kit, ODmax, the recovery.
(3) kit is placed on-20 ℃ freezing 6 days, detect once every day, measures the parameters such as IC50%, ODmax, the recovery of kit.
The result finds out that three kinds of conditions are preserved test, and each item index of this kit all conforms to quality requirements, and therefore, this kit can be 2 ℃ of-8 ℃ of preservations at least 6 months.
Claims (7)
1. a sulfadimidine enzyme-linked immunologic detecting kit has a box body, it is characterized in that comprising in the said box body:
(1) is coated with the ELISA Plate of sulfadimidine antigen;
(2) sulfadimidine specific antibody;
(3) enzyme labeling thing;
(4) sulfadimidine standard solution;
(5) A substrate colour developing liquid and B substrate colour developing liquid;
(6) stop buffer;
(7) concentrated cleaning solution;
(8) concentrate redissolution liquid;
(9) concentrated extracting solution.
2. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1; It is characterized in that said sulfadimidine antigen is obtained through the coupling of diazotising method by sulfadimidine haptens and carrier protein, said carrier protein is bovine serum albumin(BSA), human serum albumins, ovalbumin, rabbit anteserum albumen or mouse haemocyanin.
3. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1; It is characterized in that said sulfadimidine specific antibody is for preparing monoclonal antibody or polyclonal antibody by said sulfadimidine antigen by the immunization method of routine; Its working fluid is the phosphate buffer that contains 0.1-5% (w/w) skimmed milk power of pH7.4, and the sulfadimidine specific antibody is 1:2000 (w/w) with the ratio of above-mentioned working fluid.
4. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1; It is characterized in that said enzyme labeling thing is the anti-mouse of enzyme labeling or the antiantibody of anti-rabbit; Its working fluid is the phosphate buffer that contains the 0.1-5% skimmed milk power of pH7.4; The enzyme labeling thing is 1:4000 (w/w) with the ratio of above-mentioned working fluid, and marker enzyme is horseradish peroxidase or alkaline phosphatase.
5. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1 is characterized in that said sulfadimidine standard solution concentration is respectively 0 μ g/L, 0.5 μ g/L, 1.5 μ g/L, 4.5 μ g/L, 13.5 μ g/L, 40.5 μ g/L.
6. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1 is characterized in that said A substrate is the hydrogen peroxide of 0.06% (w/w), and the B substrate is 0.4% (w/w) tetramethyl benzidine.
7. sulfadimidine enzyme-linked immunologic detecting kit according to claim 1; It is characterized in that said concentrated extracting solution is 0.1M for NaOH concentration; The phosphate buffer of pH10; Said concentrated redissolution liquid is for containing the phosphate buffer of 0.1%-0.6% (w/w) DMSO, and said concentrated cleaning solution is the NaCl solution of 0.9% (w/w), and said stop buffer is the sulfuric acid solution of 2N.
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| CN2011103522531A CN102507945A (en) | 2011-12-05 | 2011-12-05 | Sulfamethazine enzyme-linked immunoassay kit |
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| CN2011103522531A CN102507945A (en) | 2011-12-05 | 2011-12-05 | Sulfamethazine enzyme-linked immunoassay kit |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344758A (en) * | 2013-07-04 | 2013-10-09 | 广西壮族自治区兽医研究所 | Chemiluminescence enzyme immunoassay detection method and kit of sulfamethazine |
| CN103524427A (en) * | 2012-07-03 | 2014-01-22 | 北京勤邦生物技术有限公司 | Preparation method as well as application of trimethoprem hapten |
| CN103808931A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit for detecting sulfadiazine and detection method thereof |
| CN106501200A (en) * | 2016-12-01 | 2017-03-15 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of unification detection method of sulfanilamide seven and test kit |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547013A (en) * | 2003-12-08 | 2004-11-17 | 中国农业大学 | Enzyme linked immuno kit for detecting sulfadimidine |
| CN1766631A (en) * | 2005-11-03 | 2006-05-03 | 北京望尔生物技术有限公司 | ELISA kit for detecting sulfanilamides residue in animal derived food |
| WO2007026000A2 (en) * | 2005-08-31 | 2007-03-08 | Novo Nordisk Health Care Ag | Human fvii monoclonal antibodies binding the gla domain and use thereof |
| CN101162230A (en) * | 2007-11-22 | 2008-04-16 | 天津科技大学 | Kit for quantitative determining enrofloxacin content in food product and testing method thereof |
| CN101226194A (en) * | 2008-01-18 | 2008-07-23 | 华南农业大学 | Malachite green vestigial ELISA detection kit and usage method thereof |
| CN101413947A (en) * | 2008-11-08 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Enzyme-linked immunologic test box for detecting melamine and preparation and detection method thereof |
| CN101650365A (en) * | 2008-08-14 | 2010-02-17 | 上海英伯肯医学生物技术有限公司 | Method and kit for quickly detecting mycobacterium tuberculosis |
| CN101750242A (en) * | 2008-12-09 | 2010-06-23 | 天津博纳艾杰尔科技有限公司 | Sample pretreatment method for detecting harmful substances in meat product and aquatic product |
| CN102079788A (en) * | 2009-11-27 | 2011-06-01 | 北京维德维康生物技术有限公司 | Method for detecting sulfanilamide medicine and special enzyme-linked immunoassay reagent kit thereof |
-
2011
- 2011-12-05 CN CN2011103522531A patent/CN102507945A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547013A (en) * | 2003-12-08 | 2004-11-17 | 中国农业大学 | Enzyme linked immuno kit for detecting sulfadimidine |
| WO2007026000A2 (en) * | 2005-08-31 | 2007-03-08 | Novo Nordisk Health Care Ag | Human fvii monoclonal antibodies binding the gla domain and use thereof |
| CN1766631A (en) * | 2005-11-03 | 2006-05-03 | 北京望尔生物技术有限公司 | ELISA kit for detecting sulfanilamides residue in animal derived food |
| CN101162230A (en) * | 2007-11-22 | 2008-04-16 | 天津科技大学 | Kit for quantitative determining enrofloxacin content in food product and testing method thereof |
| CN101226194A (en) * | 2008-01-18 | 2008-07-23 | 华南农业大学 | Malachite green vestigial ELISA detection kit and usage method thereof |
| CN101650365A (en) * | 2008-08-14 | 2010-02-17 | 上海英伯肯医学生物技术有限公司 | Method and kit for quickly detecting mycobacterium tuberculosis |
| CN101413947A (en) * | 2008-11-08 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Enzyme-linked immunologic test box for detecting melamine and preparation and detection method thereof |
| CN101750242A (en) * | 2008-12-09 | 2010-06-23 | 天津博纳艾杰尔科技有限公司 | Sample pretreatment method for detecting harmful substances in meat product and aquatic product |
| CN102079788A (en) * | 2009-11-27 | 2011-06-01 | 北京维德维康生物技术有限公司 | Method for detecting sulfanilamide medicine and special enzyme-linked immunoassay reagent kit thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524427A (en) * | 2012-07-03 | 2014-01-22 | 北京勤邦生物技术有限公司 | Preparation method as well as application of trimethoprem hapten |
| CN103524427B (en) * | 2012-07-03 | 2017-02-08 | 北京勤邦生物技术有限公司 | Preparation method as well as application of trimethoprem hapten |
| CN103808931A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Enzyme linked immunosorbent assay kit for detecting sulfadiazine and detection method thereof |
| CN103344758A (en) * | 2013-07-04 | 2013-10-09 | 广西壮族自治区兽医研究所 | Chemiluminescence enzyme immunoassay detection method and kit of sulfamethazine |
| CN103344758B (en) * | 2013-07-04 | 2016-03-23 | 广西壮族自治区兽医研究所 | Sulfadimidine chemiluminescence enzyme immunoassay detection method and kit |
| CN106501200A (en) * | 2016-12-01 | 2017-03-15 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of unification detection method of sulfanilamide seven and test kit |
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Application publication date: 20120620 |