CN102507753B - Chromatogram preparation system matched with array detector - Google Patents
Chromatogram preparation system matched with array detector Download PDFInfo
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- CN102507753B CN102507753B CN 201110322133 CN201110322133A CN102507753B CN 102507753 B CN102507753 B CN 102507753B CN 201110322133 CN201110322133 CN 201110322133 CN 201110322133 A CN201110322133 A CN 201110322133A CN 102507753 B CN102507753 B CN 102507753B
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Abstract
The invention discloses a chromatogram preparation system matched with an array detector. The chromatogram preparation system comprises the detector and a computer system connected with the detector and is characterized in that: the detector comprises a probe and an array spectrometer connected with the probe through optical fiber; and the array spectrometer receives monitored information transmitted by the probe and then transmits spectral information of the information to the computer system by adopting a full-waveband collection manner. The chromatogram preparation system matched with the array detector has the following beneficial effects that: by adopting the full-waveband collection manner, the missing of any compound arising from the wrong selection of a wavelength can be avoided; the wavelength range can be flexibly set; the absorption strengths at the same time point are automatically superposed, and weak peak forms are amplified; the data are automatically stored; the type, the purity and the concentration of biological samples can be automatically judged according to the absorption strengths under different wavelengths.
Description
Technical field
The invention belongs to chemical field, be specifically related to the preparing chromatography system in a kind of Chemical Manufacture.
Background technology
During as the detecting device of preparative chromatography instrument, preparative chromatography software all adopts single wavelength or dual wavelength to monitor at UV-vis detector.In the separation and purification process, at first the compound of preparation to be separated is analyzed or judged, selected one or two wavelength, on the basis of this wavelength, compound just judges whether to collect after occurring absorbing.But if certain unknown compound is judged the absorptance of this compound more at need, tend to establish wrong wavelength, sample does not absorb under this wavelength, thereby causes the failure of an experiment; And if multi-component biased sample, the selection meeting of wavelength is more difficult, only choose two wavelength, be easy to because wavelength selects to cause very little some compound not absorb under these two wavelength, thereby in preparation is collected, also cause the failure of an experiment because can not present absorption peak.In preparative chromatography software in the past, prepare complete after owing to can not existing preparation condition be analyzed judgement, thereby optimized purification method better, when secondary prepares because all restrictions cause purifying process to improve not quite.
Summary of the invention
The invention provides a kind of solution of the above problems, a kind of preparing chromatography system of all band scope monitoring of the array detector of arranging in pairs or groups is provided.
Technical scheme of the present invention is a kind of preparing chromatography system of the array detector of arranging in pairs or groups, it comprises detector and the computer system that is connected with described detector, it is characterized in that: described detector comprises probe and the array spectrometer that connects described probe by optical fiber, and described array spectrometer adopts all band collection mode that spectral information is wherein transferred to described computer system after receiving the monitoring information that described probe transmits.
Preferably, described array spectrometer is 1024 or 2048 array spectrometers.
Preferably, described computer system comprises:
Data memory module: receive the spectral information that described array spectrometer transmits, and it is saved to database;
Chromatogram prepares module: the spectral information that described array spectrometer transmits sampled and all band scanning, and different according to the model of described array spectrometer, the three-dimensional master map spectrum of the absorbance that all-wave is long in the making setting wavelength coverage or light intensity.
Preferably, described setting wavelength coverage comprises the wavelength coverage of following common solvent system: methanol/water, acetonitrile/water, petrol ether/ethyl acetate, chloroform/methanol.
Preferably, when described chromatogram prepares the described three-dimensional master map spectrum of module making, absorbance or the light intensity of the same time point of all band superposeed automatically, amplify the Weak Absorption peak shape.
Preferably, described chromatogram prepare module take the time as X-axis, wavelength is as Y-axis, represent that with color the 3D display mode of absorbance or light intensity shows described three-dimensional master map spectrum.
Preferably, described chromatogram prepares module and also comprises sample automatic decision submodule, is used for judging according to the characteristic absorption spectrum of common compound whether described three-dimensional master map spectrum exists common compound and concentration thereof, purity.
The preparing chromatography system of a kind of array detector of arranging in pairs or groups of the present invention compared to existing technology, has following beneficial effect: adopt all band collection mode, compound is absorbed in the full spectrum demonstration of all absorptions in 200～800nm scope, can avoids missing any compound because of the selection mistake of wavelength; Multi-solvents system automatic setting wavelength coverage and self-defined setting wavelength coverage combine, the operation of applying in a flexible way, the convenient setting; In all band scope, put at one time absorption intensity and automatically superpose, amplify faint peak shape, guarantee all to collect, do not miss any one absorption peak; Data are preserved automatically, guarantee not lose under any condition of data, and are accurate; Can also be by judging the absorption intensity under the different wave length, the energy automatic decision goes out classification, purity and the concentration of biological sample.
Description of drawings
Fig. 1 is the structural representation of the preparing chromatography system of a kind of array detector of arranging in pairs or groups of the present invention;
Fig. 2 is the display mode schematic diagram of three-dimensional master map spectrum.
Embodiment
The below is described in further detail the specific embodiment of the present invention.
As shown in Figure 1, the preparing chromatography system of a kind of array detector of arranging in pairs or groups of the present invention comprises light source, immersion cell and/or flow cell probe, array spectrometer and computer system.All connect by optical fiber between light source, probe and the array spectrometer.
The present invention can be applied to on-line monitoring and technology controlling and process in the processes such as sweat, concentration process, chemosynthesis process, separation and purification.During its work, at first by probe the real-time optical information of production run is monitored, adopted all band collection mode that spectral information is wherein transferred to computer system behind the monitoring information that the array spectrometer receiving transducer transmits.Wherein, array spectrometer is 2048 array spectrometers, can realize the long record of all-wave and analysis to multiple spectrum signals such as ultraviolet/visible absorbance, infrared absorption, Raman spectrums.
Computer system comprises:
Data memory module: the spectral information that the receiving array spectrometer transmits, and it is saved to database; To avoid causing because of fortuitous events such as outages the situation of loss of data.
Chromatogram prepares module: the spectral information that the pair array spectrometer transmits is sampled and all band scanning.Model according to described array spectrometer is different, makes the three-dimensional master map spectrum of setting the long absorbance of the interior all-wave of wavelength coverage or light intensity.Set the wavelength coverage that wavelength coverage can comprise following common solvent system: methanol/water, acetonitrile/water, petrol ether/ethyl acetate, chloroform/methanol or self-defined setting wavelength coverage.
When chromatogram prepares the described three-dimensional master map spectrum of module making, absorbance or the light intensity of the same time point of all band superposeed automatically, amplify the Weak Absorption peak shape.
As shown in Figure 2, chromatogram prepare module take the time as X-axis, wavelength is as Y-axis, represent that with color the 3D display mode of absorbance or light intensity shows described three-dimensional master map spectrum.
Chromatogram prepares module and also comprises sample automatic decision submodule, is used for judging according to the characteristic absorption spectrum of common compound whether described three-dimensional master map spectrum exists common compound and concentration thereof, purity.
The present invention adopts full spectroscopic data display analysis and judgment technology, carry out autoscan in all band scope, do not need again the absorption of compound to be selected and judged, can in all band, carry out automatic analysis, automatically collect all compounds, any one compound can be do not missed because of wavelength set, whole absorption signals of all compounds in whole wavelength coverages can be shown simultaneously.In preparation process, can effectively increase the absorption signal of Weak Absorption compound, accurately judge and collect whether the peak is single compound, find out characteristic wavelength by the absorption spectrum of compound, judge attribute and the classification of compound, secondary analysis can be more targeted after the preparation.In preparation process, get absorption intensity by the contrast ultraviolet at 254nm, 260nm, 280nm etc., can effectively obtain classification and the purity of biological sample, and calculate the concentration of its sample.
Above embodiment only is the present invention's a kind of embodiment wherein, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (5)
1. the preparing chromatography system of the array detector of arranging in pairs or groups, it comprises detector and the computer system that is connected with described detector, it is characterized in that: described detector comprises probe and the array spectrometer that connects described probe by optical fiber, described array spectrometer adopts all band collection mode that spectral information is wherein transferred to described computer system after receiving the monitoring information that described probe transmits, and described computer system comprises:
Data memory module: receive the spectral information that described array spectrometer transmits, and it is saved to database;
Chromatogram prepares module: the spectral information that described array spectrometer transmits is sampled and all band scanning, make the absorbance of 200～800nm wavelength in the setting wavelength coverage or the three-dimensional master map spectrum of light intensity, described setting wavelength coverage comprises the wavelength coverage of following common solvent system: methanol/water, acetonitrile/water, petrol ether/ethyl acetate, chloroform/methanol.
2. the preparing chromatography system of a kind of array detector of arranging in pairs or groups according to claim 1, it is characterized in that: described array spectrometer is 1024 or 2048 array spectrometers.
3. the preparing chromatography system of a kind of array detector of arranging in pairs or groups according to claim 1, it is characterized in that: when described chromatogram prepares the described three-dimensional master map spectrum of module making, absorbance or the light intensity of the same time point of all band are superposeed automatically, amplify the Weak Absorption peak shape.
4. the preparing chromatography system of a kind of array detector of arranging in pairs or groups according to claim 1 is characterized in that: described chromatogram prepare module take the time as X-axis, wavelength is as Y-axis, represent that with color the 3D display mode of absorbance or light intensity shows described three-dimensional master map spectrum.
5. the preparing chromatography system of a kind of array detector of arranging in pairs or groups according to claim 1, it is characterized in that: described chromatogram prepares module and also comprises sample automatic decision submodule, is used for judging according to the characteristic absorption spectrum of common compound whether described three-dimensional master map spectrum exists common compound and concentration thereof, purity.
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| CN 201110322133 CN102507753B (en) | 2011-10-21 | 2011-10-21 | Chromatogram preparation system matched with array detector |
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| CN102507753B true CN102507753B (en) | 2013-09-18 |
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| WO2015029508A1 (en) * | 2013-09-02 | 2015-03-05 | 株式会社島津製作所 | Chromatogram data processing device |
| CN108459120A (en) * | 2018-01-29 | 2018-08-28 | 天津博纳艾杰尔科技有限公司 | A kind of purifying preparing chromatography system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1785719A1 (en) * | 2004-08-31 | 2007-05-16 | Gl Sciences Incorporated | Optical detection method and optical detector |
| CN101566564A (en) * | 2009-06-01 | 2009-10-28 | 浙江大学 | Detecting system and method for detecting ethanol concentration by reflecting interference spectrum |
| CN101614659A (en) * | 2009-07-24 | 2009-12-30 | 上海现代中医药技术发展有限公司 | The Chinese medicine leaching process is carried out the apparatus and method of online detection and control |
| CN101614663A (en) * | 2009-07-30 | 2009-12-30 | 浙江大学 | As seen and near infrared spectrum bee pollen variety discriminating |
| CN102507468A (en) * | 2011-10-20 | 2012-06-20 | 利穗科技(苏州)有限公司 | Online monitoring system in cooperation with array detector |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP5181650B2 (en) * | 2007-12-13 | 2013-04-10 | 株式会社島津製作所 | Analysis system |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1785719A1 (en) * | 2004-08-31 | 2007-05-16 | Gl Sciences Incorporated | Optical detection method and optical detector |
| CN101566564A (en) * | 2009-06-01 | 2009-10-28 | 浙江大学 | Detecting system and method for detecting ethanol concentration by reflecting interference spectrum |
| CN101614659A (en) * | 2009-07-24 | 2009-12-30 | 上海现代中医药技术发展有限公司 | The Chinese medicine leaching process is carried out the apparatus and method of online detection and control |
| CN101614663A (en) * | 2009-07-30 | 2009-12-30 | 浙江大学 | As seen and near infrared spectrum bee pollen variety discriminating |
| CN102507468A (en) * | 2011-10-20 | 2012-06-20 | 利穗科技(苏州)有限公司 | Online monitoring system in cooperation with array detector |
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