CN102432369A - Culture base for pleurotus eryngii and preparation method thereof - Google Patents

Culture base for pleurotus eryngii and preparation method thereof Download PDF

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Publication number
CN102432369A
CN102432369A CN2011103039480A CN201110303948A CN102432369A CN 102432369 A CN102432369 A CN 102432369A CN 2011103039480 A CN2011103039480 A CN 2011103039480A CN 201110303948 A CN201110303948 A CN 201110303948A CN 102432369 A CN102432369 A CN 102432369A
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Prior art keywords
pleurotus eryngii
base
almond abalone
planting almond
abalone mushroom
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CN2011103039480A
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Inventor
李学功
李亮
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Shanxi Ak Quantum Agricultural Technology Co Ltd
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Shanxi Ak Quantum Agricultural Technology Co Ltd
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Priority to CN2011103039480A priority Critical patent/CN102432369A/en
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Abstract

The invention discloses a culture base for pleurotus eryngii and a preparation method thereof, wherein the culture base for pleurotus eryngii comprises the following raw materials in parts by mass: 80-90 parts of corncob, 8-12 parts of wheat bran, 3.5-5 parts of corn flour, 0.8-1.2 parts of gypsum and 0.8-1.2 parts of calcium carbonate. The culture base for pleurotus eryngii disclosed in the invention has advantages of low production cost and easy material availability.

Description

Planting almond abalone mushroom base-material and preparation method thereof
Technical field
The present invention relates to edible fungus culturing base-material technical field, specifically a kind of planting almond abalone mushroom base-material and preparation method thereof.
Background technology
Pleurotus eryngii (pleurotus eryngii) claims that again eryngo picks up the ears; Cryogenic thermostat firm type mushroom class in belonging to; Traditional cultivating method can only be chosen in Qiu Mo or cultivates at the beginning of the winter according to the different natural climate condition in various places, can't satisfy the anniversary consumers demand of market to the Pleurotus eryngii product.
At present, the prescription of planting almond abalone mushroom base-material is a cotton seed hull 68%, bagasse 10%, and wheat bran 20%, sucrose 1%, lime carbonate 1%, material-water ratio 1: 1.2 more than is weight percentage.This prescription is acknowledged as Xinbao mushroom culturing in China and one of fills a prescription preferably, and still, the bagasse in this prescription does not have in northern China; Though cotton seed hulls has; But price is too high, and almost the price with wheat, corn is the same, and Pleurotus eryngii production is had a strong impact on.And wherein often joined sand, join the cotton seed hulls of soil, moldy metamorphism etc. by some illegal businessmans, cause greater loss to the producer.
To producing in the above-mentioned Pleurotus eryngii anniversary and the serious problem that restricts its development of planting material shortage; The inventor actively studies and innovates; To develop the planting almond abalone mushroom base-material that a kind of raw materials cost is low, the source is abundant, solve the high problem of planting almond abalone mushroom base-material cost.
Summary of the invention
In order to solve the problems referred to above that exist in the prior art, the invention provides a kind of planting almond abalone mushroom base-material, it is low to have a cost, the advantage that raw material is easy to get.
In order to solve the problems of the technologies described above, the present invention has adopted following technical scheme:
The planting almond abalone mushroom base-material, said planting almond abalone mushroom base-material comprises the material component of following mass parts:
Figure BDA0000097352680000011
Figure BDA0000097352680000021
Further, the material component of said mass parts is:
Figure BDA0000097352680000022
Further, the water cut of said base-material is 60~65%.
Another object of the present invention is the preparation method that a kind of above-mentioned planting almond abalone mushroom base-material is provided, and it is low that the planting almond abalone mushroom base-material that preparation method of the present invention makes has a cost, the characteristics that strain growth is fast.
The technical scheme that realizes above-mentioned purpose is following:
Planting almond abalone mushroom base-material preparation method takes by weighing raw material by above-mentioned any substratum, and lime carbonate is put into water separately, mixes other component thorough mixing thoroughly, and the liming adding that then lime carbonate is formed mixes.
Further, the water cut after mixing is 60~65%, and pH is 6.5~7.
Compared with prior art, beneficial effect of the present invention is:
1, the preparation of culture material: having developed is the planting almond abalone mushroom base-material of major ingredient with the corn cob, and corn cob starting material such as wood chip, cotton seed hulls nutritive ingredient on year-on-year basis is high, and various trace elements and C/N ratio are more suitable for the Pleurotus eryngii growth; Need not prepare more auxiliary material; Rationally utilize local abundant corn cob, maize straw resource, reduced the raw materials for production cost, increased farmers' income; Reduced because of the peasant and burned the environmental pollution that agricultural waste material brings; Application obtains good result on producing, and has accomplished 5 tons of bright mushrooms of daily output, uninterrupted supply the market of anniversary.
2, water-absorbent is strong, transformation efficiency is high: corn cob metal particles shape, abundant spongy mass is arranged, and water-absorbent is strong; General planting almond abalone mushroom material moisture is 58% one 65%, and corn cob to be main cultivation base-material can reach more than 70%, can fully supply with the required moisture of sporophore growth; Differentiation is fast, transformation efficiency is high, and general planting almond abalone mushroom bag weight is calculated according to GB starting material moisture, and every bag of siccative weighs 0.5 kilogram; The 0.2-0.3 kilogram Pleurotus eryngii of growing, biological transformation ratio is 40%-60%; And corn cob to be every bag of siccative of major ingredient only need 0.4 kilogram, the 0.35-0.45 kilogram Pleurotus eryngii of growing, biological transformation ratio are 87%-115%; And the mushroom handle reaches more than 20 centimetre, and the mushroom body is delicate, and mouthfeel is clear and melodious, and quality product is high.
3, good permeability, shorten growth cycle: the corn cob granule shape is even, and the pack elasticity is fit to; Have very strong permeability, mycelial growth speed is fast, helps transforming; Shortened growth cycle; Under the equal conditions environment, conventional planting almond abalone mushroom bag mycelium growing period is 30-40 days, and the bacteria developing period of cultivation base-material of the present invention only needs 20-25 days.
4, energy-efficient: with the corn cob is the major ingredient Xinbao mushroom culturing, and production cost is low, and bacteria developing period is short; Transformation efficiency is high, has increased a year number of turnover, conventional can produce 10 prescription year and takes turns; And this prescription can produce 13 and take turns, and practiced thrift a bacterium cost, improved the turnover rate in equipment and fruiting workshop.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further detail, but not as to qualification of the present invention.
The planting almond abalone mushroom base-material comprises the material component of following mass parts: 84 parts of corn cobs, 10 parts in wheat bran, 4 parts of Semen Maydis powder, 1 part in gypsum, 1 part in lime carbonate.Above-mentioned raw materials is used water-wet, and the water cut of planting almond abalone mushroom base-material is no more than 70%.
The preparation method of planting almond abalone mushroom base-material comprises the steps: to take by weighing raw material by said components, and wherein lime carbonate is put into water separately, mixes other component thorough mixing thoroughly, and the liming adding that then lime carbonate is formed mixes.Water cut after above-mentioned raw materials mixes is no more than 70%, is preferably 60~65%.Transfer to 6.5~7 before the pack of pH value.
With the planting almond abalone mushroom base-material and the existing employing cotton seed hulls of the foregoing description cultivation base-material that is main raw material as a comparison example compare test.Test design: according to formulation ratio, each 100Kg that fills a prescription, triplicate, spring, that test repetition autumn is the same, each prescription revision test 3 times.
Content of the test:
1, mycelial growth situation and determination of yield inoculation back difference observational data different ingredients mycelium germination morning and afternoon every day and growing way, the speed of growth etc.Determination of yield weeds out all pollute bag and wait the useless bag that can not remember product after, adopt the biology efficiency method to make comparisons.
2, form and output evaluation
By supplying the accurate weighing culture material of examination prescription, the 14cm * 28cm that packs into after stirring, thick 0.04cm Vestolen PP 7052 knuckle plastics bag.Every packed siccative 300g, autoclaving, pressure are 0.15 MPa, continue 2.5h.Under aseptic condition, one of culture bag is inserted 2 grades of kinds.Postvaccinal culture bag moves into culturing room immediately, and temperature is controlled at about 25 ℃, and lucifuge is cultivated.After mycelia is covered with in the culture bag, cultivated again 7 days, make mycelia further ripe, again culture bag is moved into the fruiting room; Untie plastic ties, sack is carried on gently, and it is opened slightly; Keep mushroom room temperature at 15~18 ℃, ventilate every day 3 times, each 30min; After treating that a bag interior mycelia begins to form original hase and stretches out sack, increase space humidity, keep space relative humidity 85%~90%.
Can gather behind the mushroom body maturation, measure stem length, bacteria cover diameter, mushroom body number and every bag of Pleurotus eryngii weight when gathering.
3, quality evaluation test
3.1 the Pleurotus eryngii product that supplies test agent to adopt above-mentioned two kinds of raw materials to cultivate, air-dry appearance.
3.2 determined amino acid is measured the Pleurotus eryngii aminoacids content with the 835-50 of Hitachi type high-speed amino acid analyzer, the test foundation is NY/SOP102.
3.3 conventional nutrient component determining protein contnt is measured with the GB/T15673-1995 nitrogen determination; Lipid content is measured with the GB/T15674-1995 soxhlet extraction; Total sugar content is measured with the GB/T15672-1995 redox titration; Crude fiber content is measured with GB/T5009-1985 soda acid alcohol ether washing method.
3.4 the content of determination of trace element iron, calcium, zinc is measured with Varina-220 type atomic absorption analyzer.
Result and analysis:
1, different ingredients is to the influence of Pleurotus eryngii mycelial growth rate
Test shows: the Pleurotus eryngii mycelia all can grow on the cultivation base-material of embodiment and Comparative Examples, and the mycelial growth potential of present embodiment and the speed of growth are superior to Comparative Examples, present embodiment slightly fast 1~2 day than Comparative Examples.
2, different ingredients is to the influence of Pleurotus eryngii output (biology efficient %)
Test shows: the Pleurotus eryngii volume variance is little on the cultivation base-material of embodiment and Comparative Examples.
Different ingredients is different at the cultivation effect of different cultivation seasons, and the autumn plants output and generally is higher than the spring and plants output, analyzes its reason; Mainly be because Pleurotus eryngii belongs to the low temperature modification bacterial strain; Along with temperature reduces gradually, the fruiting phase that helps Pleurotus eryngii prolongs after autumn, so output is just high.On the contrary, Pleurotus eryngii is during the spring is planted fruiting, and along with the continuous rising of temperature, the fruiting phase shortens, and output generally reduces.
3, sporophore shape relatively
Present embodiment is slightly different with the Pleurotus eryngii form size of Comparative Examples, and is many with every bag of number of corncob cultivation, individual smaller; Number with cottonseed shell cultivation is few, and is individual big.The strong degree of bacterial context, mouthfeel, storage endurance etc. are also distinguished not quite.
4, attributional analysis
4.1 aminoacids content and composition
The Pleurotus eryngii aminoacids content of present embodiment and Comparative Examples cultivation is respectively 13.06mg and 12.72mg.In amino acid is formed, all can detect 17 seed amino acids, wherein essential amino acids content is respectively 5.52mg and 5.5mg.
4.2 conventional nutrient composition content
The Pleurotus eryngii protein of present embodiment and Comparative Examples cultivation is respectively 18.3% and 20.5%; Lipid content is respectively 3.78% and 3.12%; It is 6.4% and 5.9% that polysaccharide content is respectively, and crude fiber content is respectively 14.45% and 13.30%, and ash oontent is respectively 5.7% and 6.0%.
4.3 micronutrient levels
The dried mushroom content of the every hectogram of Pleurotus eryngii mineral element of present embodiment and Comparative Examples cultivation is respectively: calcium 135.5 and 148.7 micrograms, magnesium 1305.2 and 1278.9 micrograms, copper 104 and 112 micrograms, zinc 74.4 and 75.8 micrograms, manganese 11.3 and 13.2 micrograms.Iron 111.5 and 107.9 micrograms, zinc are 1.56% and 1.74%, and phosphorus is 1.54 and 1.71%.
Conclusion
Test-results shows, replaces cotton seed hulls to come Xinbao mushroom culturing feasible fully as the main raw material of cultivation base-material with aboundresources, cheap corn cob.
Above embodiment is merely exemplary embodiment of the present invention, is not used in restriction the present invention, and protection scope of the present invention is defined by the claims.Those skilled in the art can make various modifications or be equal to replacement the present invention in essence of the present invention and protection domain, this modification or be equal to replacement and also should be regarded as dropping in protection scope of the present invention.

Claims (5)

1. the planting almond abalone mushroom base-material is characterized in that, said planting almond abalone mushroom base-material comprises the material component of following mass parts:
Figure FDA0000097352670000011
2. planting almond abalone mushroom base-material according to claim 1 is characterized in that, the material component of said mass parts is:
Figure FDA0000097352670000012
3. planting almond abalone mushroom base-material according to claim 1 is characterized in that, the water cut of said base-material is 60~65%.
4. planting almond abalone mushroom base-material preparation method; It is characterized in that take by weighing raw material by claim 1 or 2 arbitrary described cultivation base-materials, wherein lime carbonate is put into water separately; Mix other component thorough mixing thoroughly, the liming adding that then lime carbonate is formed mixes.
5. planting almond abalone mushroom base-material preparation method according to claim 4 is characterized in that the water cut after mixing is 60~65%, and pH is 6.5~7.
CN2011103039480A 2011-10-10 2011-10-10 Culture base for pleurotus eryngii and preparation method thereof Pending CN102432369A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103416216A (en) * 2012-05-23 2013-12-04 大连良农农业产业发展有限公司 Formula for cultivating pleurotus eryngii liquid strain with corn stalks as base materials and preparation method thereof
CN104926467A (en) * 2015-06-10 2015-09-23 柳州市宝杨种植专业合作社 Pleurotus eryngii culture material
CN107434537A (en) * 2016-05-26 2017-12-05 桂林洁宇环保科技有限责任公司 A kind of new planting almond abalone mushroom material

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CN101717298A (en) * 2009-10-30 2010-06-02 山东芳绿农业科技有限公司 Pleurotus eryngii cultivation method and cultivation material thereof
CN101983551A (en) * 2010-11-30 2011-03-09 忻州市汇恒农林牧综合开发有限公司 Cultivation method of king oyster mushroom

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CN101456772A (en) * 2008-12-16 2009-06-17 许忠 Culture medium of pleurotus eryngii and preparation thereof
CN101717298A (en) * 2009-10-30 2010-06-02 山东芳绿农业科技有限公司 Pleurotus eryngii cultivation method and cultivation material thereof
CN101983551A (en) * 2010-11-30 2011-03-09 忻州市汇恒农林牧综合开发有限公司 Cultivation method of king oyster mushroom

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103416216A (en) * 2012-05-23 2013-12-04 大连良农农业产业发展有限公司 Formula for cultivating pleurotus eryngii liquid strain with corn stalks as base materials and preparation method thereof
CN103416216B (en) * 2012-05-23 2016-01-20 大连良农农业产业发展有限公司 Corn stalk makes formula of base-material Xinbao mushroom culturing liquid spawn and preparation method thereof
CN104926467A (en) * 2015-06-10 2015-09-23 柳州市宝杨种植专业合作社 Pleurotus eryngii culture material
CN107434537A (en) * 2016-05-26 2017-12-05 桂林洁宇环保科技有限责任公司 A kind of new planting almond abalone mushroom material

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Application publication date: 20120502