CN102313729A - Portable Raman spectrometer - Google Patents
Portable Raman spectrometer Download PDFInfo
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- CN102313729A CN102313729A CN201110217015A CN201110217015A CN102313729A CN 102313729 A CN102313729 A CN 102313729A CN 201110217015 A CN201110217015 A CN 201110217015A CN 201110217015 A CN201110217015 A CN 201110217015A CN 102313729 A CN102313729 A CN 102313729A
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Abstract
The invention discloses a portable Raman spectrometer. The spectrometer comprises a main system which comprises a laser, a laser probe, an analysis module and a first imaging module, wherein the laser, the laser probe, the analysis module and the first imaging module are sequentially connected; and a micro-area system which comprises an optical system of a microscope, wherein the optical system of the microscope which is connected with the laser is also connected with the analysis module, and the lower part of the optical system of the microscope is provided with a sample testboard which can bear a slide glass. In the invention, the portable Raman spectrometer allows the demand of a sample amount to be substantially reduced by means of the micro-area system and allows the routine analysis of a large sample amount to be carried out when only the main system is used for analysis. The portable Raman spectrometer which is a supplement to existing portable Raman spectrometers allows the development of the portable Raman spectrometers to be promoted.
Description
Technical field
The present invention relates to a kind of analytical instrument, relate in particular to a kind of Portable Raman optical spectrum appearance.
Background technology
Raman scattering is a kind of of light scattering phenomenon; Elastic collision and inelastic collision can take place when the light inlet photon of homogeneous beam and interaction of molecules; In the elastic collision process; Photon and intermolecular does not have energy exchange, and photon only changes direction of motion and do not change frequency, and this scattering process is called Rayleigh scattering.And in the inelastic collision process; Energy exchange takes place between photon and the molecule; Photon not only changes direction of motion, and the part energy of photon passes to molecule simultaneously, and perhaps the vibration of molecule and rotational energy pass to photon; Thereby changed the frequency of photon, this scattering process is called Raman scattering.The intensity of the number of Raman line, the size of shift value and bands of a spectrum etc. are all with the vibration of material molecule with rotate relevantly, and these information have all reflected the conformation and the residing environment thereof of molecule.
Raman spectroscopy is discerned and is distinguished the different structures of matter from the molecular vibration spectrum of material, becomes the effective means of research material molecular structure.The analytical approach of Raman spectrum need not carried out pre-treatment to sample, does not have the preparation process of sample yet, has avoided the generation of some errors, and advantage such as simple to operate in analytic process, minute weak point, sensitivity height.At present, Raman spectrum has been widely used in fields such as material, chemical industry, oil, macromolecule, biology, environmental protection, geology.
Raman spectrometer is used for the rectangular scattered light of collection and detection and incident light, and scattered light spectrum is analyzed and handled, thereby draws the molecular structure of material.But since Raman signal very a little less than, require the optical devices of sophisticated, be unfavorable for in-site measurement; And new working method has been opened up in the fibre-optic design that develops into optical instrument; Make spectrometer can take the mode of popping one's head in and separating, simplified the structure of optical system, exempted loaded down with trivial details adjustment process with apparatus subject; Improved dirigibility and the reliability used, made the Raman spectrum on-line measurement become possibility.This Raman spectrum that is used for on-line measurement is exactly the Portable Raman optical spectrum appearance.
Current two kinds of popular probes, a kind of is insertion type probe, on the principle, the optical fiber that is arranged side by side by two-way constitutes.Probe directly immerses sample during use, and one the tunnel is used for laser is sent to sample, and another road receives by the scattered light of sample generation and with it and passes receiving system back.Another kind is the external probe, and probe places outside the sample during use, and two-way optical fiber is arranged, and exciting light reaches probe by one road optical fiber from light source, converges at sample through the optical system of probe and the optical transmission window of sampling receptacle.The scattered light that comes from sample is popped one's head in to be collected and reaches receiving system through another road optical fiber.But above-mentioned dual mode all has problems, and it is bigger to analyze needed sample size exactly, has so just limited the application of Raman spectrometer again, has also limited the development of Portable Raman optical spectrum appearance.
Summary of the invention
The present invention is directed to above-mentioned technical matters, designed and developed a kind of Portable Raman optical spectrum appearance.Among the present invention, Raman spectroscopy system is gone in the microcell system integration, and formed the Portable Raman optical spectrum appearance on this basis.This Portable Raman optical spectrum appearance can be realized the micron order analysis to sample surfaces by the microcell system, greatly reduces the demand of sample size; When only using main system to analyze, can carry out conventional large sample quantitative analysis again.Therefore, the present invention replenishes a kind of of Portable Raman optical spectrum appearance, can promote to be used for the development of the Portable Raman optical spectrum appearance of on-line analysis.
Technical scheme provided by the invention is:
A kind of Portable Raman optical spectrum appearance comprises:
Main system, it includes laser instrument, laser probe, analysis module and first image-forming module, and wherein, said laser instrument, laser probe, said analysis module and said first image-forming module are connected successively; And
The microcell system; It includes microscope optical system, and wherein, said microscope optical system is connected in said laser instrument; Said microscope optical system also is connected in said analysis module, and the below of said microscope optical system is provided with the sample test platform that can carry microslide.
Preferably; In the described Portable Raman optical spectrum appearance; The laser instrument of said main system, analysis module and first image-forming module and said microcell system all are arranged in the casing, and said laser probe is positioned at the said casing outside, wherein; Said casing is being provided with a long and narrow hole with the corresponding position of said sample test platform; Said sample test platform is through the said long and narrow hole and the exterior of said casing, and a side of said casing also is provided with display, and said display is connected with said first image-forming module.
Preferably, in the described Portable Raman optical spectrum appearance, said casing top also is provided with a pulling apparatus.
Preferably, described Portable Raman optical spectrum appearance also comprises: control chip; Wherein, said main system is connected in said control chip, and said main system is controlled by said control chip; Said laser instrument sends laser to said laser probe, and said analysis module is gathered and analyzed Raman signal, and said first image-forming module shows the Raman spectrogram; And; Said microcell system also is connected in said control chip, and said microcell system is controlled by said control chip, and said laser instrument sends laser to said microscope optical system; Said microscope optical system is the sample of laser focusing on the microslide that is positioned at said sample test platform, and Raman signal is passed to said analysis module.
Preferably; Described Portable Raman optical spectrum appearance, said microcell system also comprises the scanister and second image-forming module, wherein; Said scanister is connected in said control chip; Sample on the microslide that is positioned at said sample test platform scans, and said second image-forming module is connected in said analysis module, and said second image-forming module also is connected in said control chip; Said second image-forming module receives said control chip control to show the Raman spectrogram, and the display of said casing also is connected with said second image-forming module.
Preferably, described Portable Raman optical spectrum appearance also comprises: power supply chip; Said power supply chip is connected in said control chip; Be connected in said main system and microcell system simultaneously, listen and to state power supply chip and controlled by said control chip, to said main system power supply; Or said power supply chip controls by said control chip, simultaneously to said main system and the power supply of microcell system.
Preferably, described Portable Raman optical spectrum appearance, said microcell system also comprises slide unit, it is connected with said microscope optical system, drives said microscope optical system and moves up and down with respect to said sample test platform.
Preferably, described Portable Raman optical spectrum appearance, said microcell system also comprises slide unit, it is connected with said sample test platform, drives said sample test platform and moves up and down with respect to said microscope optical system.
Portable Raman optical spectrum appearance of the present invention is gone into Raman spectroscopy system with the microcell system integration, and has formed the Portable Raman optical spectrum appearance on this basis.This Portable Raman optical spectrum appearance can be realized the micron order analysis to sample surfaces by the microcell system, greatly reduces the demand of sample size; When only using main system to analyze, can carry out conventional large sample quantitative analysis again.Therefore, the present invention replenishes a kind of of Portable Raman optical spectrum appearance, can promote to be used for the development of the Portable Raman optical spectrum appearance of on-line analysis.
Description of drawings
Fig. 1 is the inner structure synoptic diagram of Portable Raman optical spectrum appearance of the present invention.
Fig. 2 is the external structure synoptic diagram of Portable Raman optical spectrum appearance of the present invention.
Embodiment
Below in conjunction with accompanying drawing the present invention is done further detailed description, can implement according to this with reference to the instructions literal to make those skilled in the art.
As depicted in figs. 1 and 2; The present invention provides a kind of Portable Raman optical spectrum appearance, comprising: main system, and it includes laser instrument 3, laser probe, analysis module 2 and first image-forming module; Wherein, said laser instrument 3, laser probe, analysis module 2 are connected with first image-forming module successively; And microcell system; It includes microscope optical system 6, and wherein, said microscope optical system 6 is connected in said laser instrument 3; Said microscope optical system 6 also is connected in said analysis module 2, and the below of said microscope optical system 6 is provided with the sample test platform 11 that can carry microslide.
Above-mentioned Portable Raman optical spectrum appearance is exactly an integrated microcell system on the Raman spectrum main system.Above-mentioned main system has comprised laser instrument 3, laser probe, analysis module 2 and the first image-forming module (not shown), and above-mentioned four parts connect successively.Wherein, first image-forming module is integrated in analysis module 2 inside.When using main system to carry out online detection; The laser that laser instrument 3 generates shines in sample surfaces via laser probe 8; Laser probe is collected the scattered light that comes from sample surfaces simultaneously; And be passed to analysis module 2 this Raman signal is gathered and analyzed, analysis result is shown in display 15 via first image-forming module with the form of Raman spectrogram.
Above-mentioned analytic process mainly is to the bigger situation of sample size, in sample size hour, then starts the microcell system.When using the microcell system; The laser that laser instrument 3 is taken place gets into the light path of microscope optical system 6, and the microslide that carries sample is positioned at sample test platform 7,6 pairs of laser focusings of microscope optical system; And will shine in sample; Sample scattering light is passed to analysis module 2 via microscope optical system 6 again, thereby can gather and analyze Raman signal, and analysis result is shown in display 15 by second image-forming module 1 with the form of Raman spectrogram.6 pairs of laser of microscope optical system have focusing function, dwindle surveyed area, have also just reduced the needs to sample size.
Generally speaking, the Portable Raman optical spectrum appearance does not possess the microcell system, and microcell system that Portable Raman optical spectrum appearance of the present invention is integrated has realized the micron order of sample surfaces is detected, and makes that the application of Portable Raman optical spectrum appearance in detecting at the scene is more extensive.
In one embodiment, the course of work of main system and microcell system all depends on control chip 4.The laser instrument 3 that main system comprised, analysis module 2 and first image-forming module all are connected in control chip 4; The control of controlled chip 4; Laser instrument 3 sends laser to laser probe; 2 pairs of analysis modules come from the scattered light of sample to be gathered and analyzes, and forming similarly is to rely on first image-forming module to realize, through first image-forming module Raman spectrogram is shown in display 15.
4 control microcell systems get into duty through control chip.In one embodiment, microscope optical system 6 adopts confocal microscopy.Therefore, the microcell system also contains scanister, and scanister has been realized the scanning observation to sample; With confocal microscopy; Sample is carried out nondestructively demixing scan, reaches the effect of " optical section ", to sample carry out three-dimensional pointwise, line by line, successively micro-zone analysis.The Raman signal of the confocal pinhole through microscope optical system 6; Through assembling, to the entrance slit of the monochromator of analysis module 2, by monochromator splitting, imaging; Be shown as the Raman spectrogram through second image-forming module 1, form " topomap " that so-called sample material component distributes.This wherein, the switching between the control of the control of the course of work of microscope optical system 6, the course of work of scanister and first image-forming module and second image-forming module all is to be realized by control chip 4.
In the described Portable Raman optical spectrum appearance; The laser instrument 3 of said main system, analysis module 2 and first image-forming module and said microcell system all are arranged in the casing 13; Said laser probe is positioned at said casing 13 outsides; Wherein, said casing 13 is being provided with a long and narrow hole 12 with said sample test platform 11 corresponding positions, and said sample test platform 11 is through the exterior of said long and narrow hole 12 with said casing 13; One side of said casing 13 also is provided with display 15, and said display 15 is connected with said first image-forming module.
In the described Portable Raman optical spectrum appearance, said casing 13 tops also are provided with a pulling apparatus 14.
Described Portable Raman optical spectrum appearance also comprises: control chip 5, wherein; Said main system is connected in said control chip 5, and said main system receives said control chip 5 controls, and said laser instrument 3 sends laser to said laser probe; 2 pairs of Raman signals of said analysis module are gathered and are analyzed; Said first image-forming module shows the Raman spectrogram, and said microcell system also is connected in said control chip 5; Said microcell system receives said control chip 5 controls; Said laser instrument 3 sends laser to said microscope optical system 6, and said microscope optical system 6 is the sample of laser focusing on the microslide that is positioned at said sample test platform 11, and Raman signal is passed to said analysis module 2.
In the described Portable Raman optical spectrum appearance; Said microcell system also comprises the scanister and second image-forming module 1; Wherein, said scanister is connected in said control chip 4, and the sample on the microslide that is positioned at said sample test platform 11 is scanned; Said second image-forming module 1 is connected in said analysis module 2; Said second image-forming module 1 also is connected in said control chip 4, and said second image-forming module 1 receives said control chip 4 controls to show the Raman spectrogram, and the display 15 of said casing 13 also is connected with said second image-forming module 1.
Described Portable Raman optical spectrum appearance; Also comprise: power supply chip 4, said power supply chip 4 is connected in said control chip 5, is connected in said main system and microcell system simultaneously; Said power supply chip 4 receives said control chip 5 controls; To the power supply of said main system, or said power supply chip 4 receives said control chip 5 controls, supplies power with the microcell system to said main system simultaneously.
In the described Portable Raman optical spectrum appearance, said microcell system also comprises slide unit, and it is connected with said microscope optical system 6, drives said microscope optical system 6 and moves up and down with respect to said sample test platform 11.
In the described Portable Raman optical spectrum appearance, said microcell system also comprises slide unit, and it is connected with said sample test platform 11, drives said sample test platform 11 and moves up and down with respect to said microscope optical system 6.
Although embodiment of the present invention are open as above; But it is not restricted to listed utilization in instructions and the embodiment; It can be applied to various suitable the field of the invention fully, for being familiar with those skilled in the art, can easily realize other modification; Therefore under the universal that does not deviate from claim and equivalency range and limited, the legend that the present invention is not limited to specific details and illustrates here and describe.
Claims (8)
1. a Portable Raman optical spectrum appearance is characterized in that, comprising:
Main system, it includes laser instrument, laser probe, analysis module and first image-forming module, and wherein, said laser instrument, laser probe, said analysis module and said first image-forming module are connected successively; And
The microcell system; It includes microscope optical system, and wherein, said microscope optical system is connected in said laser instrument; Said microscope optical system also is connected in said analysis module, and the below of said microscope optical system is provided with the sample test platform that can carry microslide.
2. Portable Raman optical spectrum appearance as claimed in claim 1; It is characterized in that; The laser instrument of said main system, analysis module and first image-forming module and said microcell system all are arranged in the casing, and said laser probe is positioned at the said casing outside, wherein; Said casing is being provided with a long and narrow hole with the corresponding position of said sample test platform; Said sample test platform is through the said long and narrow hole and the exterior of said casing, and a side of said casing also is provided with display, and said display is connected with said first image-forming module.
3. Portable Raman optical spectrum appearance as claimed in claim 2 is characterized in that, said casing top also is provided with a pulling apparatus.
4. Portable Raman optical spectrum appearance as claimed in claim 2 is characterized in that, also comprises: control chip; Wherein, said main system is connected in said control chip, and said main system is controlled by said control chip; Said laser instrument sends laser to said laser probe, and said analysis module is gathered and analyzed Raman signal, and said first image-forming module shows the Raman spectrogram; And; Said microcell system also is connected in said control chip, and said microcell system is controlled by said control chip, and said laser instrument sends laser to said microscope optical system; Said microscope optical system is the sample of laser focusing on the microslide that is positioned at said sample test platform, and Raman signal is passed to said analysis module.
5. Portable Raman optical spectrum appearance as claimed in claim 4; It is characterized in that said microcell system also comprises the scanister and second image-forming module, wherein; Said scanister is connected in said control chip; Sample on the microslide that is positioned at said sample test platform scans, and said second image-forming module is connected in said analysis module, and said second image-forming module also is connected in said control chip; Said second image-forming module receives said control chip control to show the Raman spectrogram, and the display of said casing also is connected with said second image-forming module.
6. like claim 4 or 5 described Portable Raman optical spectrum appearance, it is characterized in that, also comprise: power supply chip; Said power supply chip is connected in said control chip; Be connected in said main system and microcell system simultaneously, said power supply chip is controlled by said control chip, to said main system power supply; Or said power supply chip controls by said control chip, simultaneously to said main system and the power supply of microcell system.
7. Portable Raman optical spectrum appearance as claimed in claim 2 is characterized in that, said microcell system also comprises slide unit, and it is connected with said microscope optical system, drives said microscope optical system and moves up and down with respect to said sample test platform.
8. Portable Raman optical spectrum appearance as claimed in claim 2 is characterized in that, said microcell system also comprises slide unit, and it is connected with said sample test platform, drives said sample test platform and moves up and down with respect to said microscope optical system.
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| CN 201110217015 CN102313729B (en) | 2011-07-29 | 2011-07-29 | Portable Raman spectrometer |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424392A (en) * | 2012-05-24 | 2013-12-04 | 黄书伟 | Wavelength division SRS microscope |
| CN104865279A (en) * | 2014-02-26 | 2015-08-26 | 清华大学 | Customs supervision item rapid-inspection equipment and method |
| CN108323182A (en) * | 2018-01-02 | 2018-07-24 | 深圳达闼科技控股有限公司 | A kind of spectral measurement system |
| CN109745006A (en) * | 2019-01-31 | 2019-05-14 | 北京超维景生物科技有限公司 | Separate type adsorbent equipment, microscope detection device and laser scanning microscope |
| CN110186904A (en) * | 2019-05-10 | 2019-08-30 | 无锡瑞生医疗科技有限公司 | Micro-Raman spectroscopy |
| CN111044500A (en) * | 2019-12-30 | 2020-04-21 | 北京华泰诺安探测技术有限公司 | Raman probe capable of adjusting annular detection area |
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| CN101514964A (en) * | 2009-03-27 | 2009-08-26 | 福州高意光学有限公司 | A material detector based on Raman spectroscopy |
| CN202182863U (en) * | 2011-07-29 | 2012-04-04 | 北京华泰诺安科技有限公司 | Portable Raman spectrometer |
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2011
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| US6545755B1 (en) * | 1999-11-26 | 2003-04-08 | Jeol Ltd. | Micro-Raman spectroscopy system for identifying foreign material on a semiconductor wafer |
| CN101514964A (en) * | 2009-03-27 | 2009-08-26 | 福州高意光学有限公司 | A material detector based on Raman spectroscopy |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424392A (en) * | 2012-05-24 | 2013-12-04 | 黄书伟 | Wavelength division SRS microscope |
| CN104865279A (en) * | 2014-02-26 | 2015-08-26 | 清华大学 | Customs supervision item rapid-inspection equipment and method |
| CN108323182A (en) * | 2018-01-02 | 2018-07-24 | 深圳达闼科技控股有限公司 | A kind of spectral measurement system |
| CN109745006A (en) * | 2019-01-31 | 2019-05-14 | 北京超维景生物科技有限公司 | Separate type adsorbent equipment, microscope detection device and laser scanning microscope |
| CN109745006B (en) * | 2019-01-31 | 2024-03-15 | 北京超维景生物科技有限公司 | Separation type adsorption device, microscope detection device and laser scanning microscope |
| CN110186904A (en) * | 2019-05-10 | 2019-08-30 | 无锡瑞生医疗科技有限公司 | Micro-Raman spectroscopy |
| CN111044500A (en) * | 2019-12-30 | 2020-04-21 | 北京华泰诺安探测技术有限公司 | Raman probe capable of adjusting annular detection area |
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Effective date of registration: 20160511 Address after: 101318 Shunyi District airport economic core area Yuhua Road, building 5, room 4, floor 002, room 28, No. Patentee after: BEIJING HT NOVA TESTING TECHNOLOGY CO., LTD. Address before: 101100 Beijing city Tongzhou District Cuiping Building No. 20, taking 20 Patentee before: Beijing Mande Technology Co. Ltd. |